LOC101342847 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101342847
Entrez Gene ID	101342847
Full Name	toll-like receptor 2
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004378810.2	XP_004378867.1	toll-like receptor 2
XM_023733515.1	XP_023589283.1	toll-like receptor 2

Pathways from BioSystems
Gene Ontology

The following LOC101342847 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101342847 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr175898	XM_004378810.2 Latest version!	Trichechus manatus latirostris toll-like receptor 2 (LOC101342847), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTr175898	XM_023733515.1 Latest version!	Trichechus manatus latirostris toll like receptor 2 (LOC101342847), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101342847 ( XM_004378810.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004378867.1 Trichechus manatus latirostris toll-like receptor 2 (LOC101342847), mRNA.

Price & Availability↑

CloneID	OTr175898
Clone ID Related Accession (Same CDS sequence)	XM_004378810.2 , XM_023733515.1
Accession Version	XM_004378810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	toll-like receptor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443995.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004378810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCATG CTTTGTGGAT ACTGTGGGCC TTGGGGGCCA TAATCACCCT CTCCAAAGGA 
GGGTCCCCTG ATCAGGCTTT TCTGTCTTGT GACCCCACGG GTGTCTGCGA TGGCTCCTCC 
AGGTCTTTGA ACTCCATTCC CTCAGGGCTC ACAGAAGCTG TGAAAAGCCT TGACCTGTCC 
AACAACAAGA TTGCATATGT TGGCAACACT GACCTTCAGT TGTGTGTGAA CCTCAAAGCT 
CTGATTCTGA AGTCCAGTGA AATTAATAGA ATAGATGAAG ATGCTTTTCT TCCCCTTGGA 
AGTCTTGAAC ATTTGGACTT ATCCTATAAT TACTTATCTA ATTTATCATC CTCCTGGTTC 
AGGCCCCTTT CTTCCTTGCA GTTCTTAAGC TTACTGGGAA ATCTTTACAA AGAACTTGGG 
GAAACATCTC TTTTTTCTCA TCTCACAAAT TTGCGAGTCC TGAAAGTAGG AAACATTTAC 
ACCTCTAAGA TTCAGAAAAA GGATTTTACT GGAATAACTT TTCTTAAGGA ACTTGAGATT 
GATGCTTCAA ATCTCCAGAG TTATGAGCCA GAAAGCTTGA AGTCAATTCA GAATATAAGT 
CATCTGATCC TTCATATGAG GCAGCCTATT TTACTGCCGG CGACTTTTGT AGATCTTTTA 
AGTTCCTTGG AATATTTTGA ACTGAGAAAT ACTGATTTGC ACACTTTGCA TTTTCCAGAA 
CTACCCATCA GTGAAACCAA CCCACTGATT AAAAAGTTCA CCTTTAGGAA TGTGGAAATC 
ACTGATGAAA GTTTTAATGA AGTTATGAAA CTGTTCAGTT CTGCCTCTGA ACTTTCAGAA 
TTAGAGTTTG ATGACTGTAC CCTTAATGGA ATCGGTAATT TTGATTCATC TGTTATAGAG 
AAAATTAAAG ATCCAGGTAA AGTAGAAACA TTAATAATAC GGAAGTTGCA TATTCCACAG 
TTTTACTTAT TTAATGATTT GAGTAGTATA TATTCACTCA CAGGAAACCT TAAAAGAATC 
ACAATAGAAA ACAGCAAGGT TTTTCTGGTT CCTTGTTTAC TTTCACAGCA TTTAAAATCA 
TTAGAATACT TGGATCTGAG TGAAAATCTG ATGGTTGAAG AATACTTGAA AAATTCAGCT 
TGTAAGGATG CCTGGCCCTC CCTACAAACC TTAATTTTAA GGCAAAATCA CCTGACATCA 
TTAGAGAAAA CTGGAGAAAC TTTGCTTACT CTCAAAAACC TGACTAGCCT TGATATCAGT 
AAGAATAGTT TTAAGTCTAT GCCTGAAAAT TGCTGGTGGC CAGAAAAGAT GAAATATTTG 
AACTTATCCA GCACACAAAT ACATAGTTTA ACCTATTGTA TTCCCCAGAC ACTGGAAATT 
TTAGATGTTA GCAATAACAA TCTCAATTCA TTTTCTTTGA TGTTACCACA ACTCAGAGAA 
CTTTATATTT CCAGAAATAA GTTGAAGACT CTACCAGATT CCACCTTCTT ACCCATGTTA 
CTAGTCATGA GAATCAGCAG GAACACAATA AATACTTTCT CTAAGGAGCA ATTTGAATCC 
CTTCAAAAAC TGAAAACATT GGAAGCGGGT GGAAACAACT TCATTTGCTC CTGTGAATTC 
CTGTCTTTCA TGCAGGAGCA GCAAGCACTG ACTAAGGTCT TGGTTGACTG GCCAGAAAAC 
TACCTGTGTG ACTCTCCATC CAATGTGCGG GGCCAGCGAG TTCAGGACAC GCGCCTCTCG 
GTTGCTGAGT GCCACAGGGC AGCCCTGGTG TCTGCTGTGT GCTGTGCCCT TCTTCTGTTC 
ATCTTGCTCA TGGGTGTTCT GTGCCACCGT CTCCATGGGC TGTGGTACAT GAAAATGATG 
TGGGCCTGGC TCCAGGCCAA AAGGAAGCCC AAGAAGGCAC ACAACAGGGA CATCTGTTAC 
GACGCTTTTG TTTCTTATAG TGAAAGGGAT TCCTACTGGG TGGAGAGCCT TATGGTCCAG 
GAGCTGGAGA ATTTCGACCC CCCCTTTAGG TTATGTCTTC ATAAGCGGGA CTTTGTTCCT 
GGCAAGTGGA TTATTGACAA TATCATTGAC TGCATTGAAA AGAGTCACAA AACCGTCTTT 
GTGCTTTCTG AAAACTTTGT GAAGAGTGAG TGGTGCAAGT ATGAACTGGA CTTCTCCCAT 
TTTCGTCTCT TTGATGAGAA CAATGATGCT GCCATTCTCA TTCTTCTGGA GCCCATTGAG 
AAGAAAGCCA TTCCCCAGCG GTTCTGTAAA CTGCGGAAGA TCATGAATAC CAAGACCTAC 
CTGGAGTGGC CCAATGATGA AGCTCAGCAG CAAGGGTTTT GGCTAAATTT GAGAACTGCA 
ATAAAGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004378867.1
CDS	28..2379
Translation	MSHALWILWALGAIITLSKGGSPDQAFLSCDPTGVCDGSSRSLNSIPSGLTEAVKSLDLSNNKIAYVGNTDLQLCVNLKALILKSSEINRIDEDAFLPLGSLEHLDLSYNYLSNLSSSWFRPLSSLQFLSLLGNLYKELGETSLFSHLTNLRVLKVGNIYTSKIQKKDFTGITFLKELEIDASNLQSYEPESLKSIQNISHLILHMRQPILLPATFVDLLSSLEYFELRNTDLHTLHFPELPISETNPLIKKFTFRNVEITDESFNEVMKLFSSASELSELEFDDCTLNGIGNFDSSVIEKIKDPGKVETLIIRKLHIPQFYLFNDLSSIYSLTGNLKRITIENSKVFLVPCLLSQHLKSLEYLDLSENLMVEEYLKNSACKDAWPSLQTLILRQNHLTSLEKTGETLLTLKNLTSLDISKNSFKSMPENCWWPEKMKYLNLSSTQIHSLTYCIPQTLEILDVSNNNLNSFSLMLPQLRELYISRNKLKTLPDSTFLPMLLVMRISRNTINTFSKEQFESLQKLKTLEAGGNNFICSCEFLSFMQEQQALTKVLVDWPENYLCDSPSNVRGQRVQDTRLSVAECHRAALVSAVCCALLLFILLMGVLCHRLHGLWYMKMMWAWLQAKRKPKKAHNRDICYDAFVSYSERDSYWVESLMVQELENFDPPFRLCLHKRDFVPGKWIIDNIIDCIEKSHKTVFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPNDEAQQQGFWLNLRTAIKS

Target ORF information:

RefSeq Version	XM_004378810.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris toll-like receptor 2 (LOC101342847), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004378810.2

ORF Insert Sequence:

ATGTCGCATG CTTTGTGGAT ACTGTGGGCC TTGGGGGCCA TAATCACCCT CTCCAAAGGA 
GGGTCCCCTG ATCAGGCTTT TCTGTCTTGT GACCCCACGG GTGTCTGCGA TGGCTCCTCC 
AGGTCTTTGA ACTCCATTCC CTCAGGGCTC ACAGAAGCTG TGAAAAGCCT TGACCTGTCC 
AACAACAAGA TTGCATATGT TGGCAACACT GACCTTCAGT TGTGTGTGAA CCTCAAAGCT 
CTGATTCTGA AGTCCAGTGA AATTAATAGA ATAGATGAAG ATGCTTTTCT TCCCCTTGGA 
AGTCTTGAAC ATTTGGACTT ATCCTATAAT TACTTATCTA ATTTATCATC CTCCTGGTTC 
AGGCCCCTTT CTTCCTTGCA GTTCTTAAGC TTACTGGGAA ATCTTTACAA AGAACTTGGG 
GAAACATCTC TTTTTTCTCA TCTCACAAAT TTGCGAGTCC TGAAAGTAGG AAACATTTAC 
ACCTCTAAGA TTCAGAAAAA GGATTTTACT GGAATAACTT TTCTTAAGGA ACTTGAGATT 
GATGCTTCAA ATCTCCAGAG TTATGAGCCA GAAAGCTTGA AGTCAATTCA GAATATAAGT 
CATCTGATCC TTCATATGAG GCAGCCTATT TTACTGCCGG CGACTTTTGT AGATCTTTTA 
AGTTCCTTGG AATATTTTGA ACTGAGAAAT ACTGATTTGC ACACTTTGCA TTTTCCAGAA 
CTACCCATCA GTGAAACCAA CCCACTGATT AAAAAGTTCA CCTTTAGGAA TGTGGAAATC 
ACTGATGAAA GTTTTAATGA AGTTATGAAA CTGTTCAGTT CTGCCTCTGA ACTTTCAGAA 
TTAGAGTTTG ATGACTGTAC CCTTAATGGA ATCGGTAATT TTGATTCATC TGTTATAGAG 
AAAATTAAAG ATCCAGGTAA AGTAGAAACA TTAATAATAC GGAAGTTGCA TATTCCACAG 
TTTTACTTAT TTAATGATTT GAGTAGTATA TATTCACTCA CAGGAAACCT TAAAAGAATC 
ACAATAGAAA ACAGCAAGGT TTTTCTGGTT CCTTGTTTAC TTTCACAGCA TTTAAAATCA 
TTAGAATACT TGGATCTGAG TGAAAATCTG ATGGTTGAAG AATACTTGAA AAATTCAGCT 
TGTAAGGATG CCTGGCCCTC CCTACAAACC TTAATTTTAA GGCAAAATCA CCTGACATCA 
TTAGAGAAAA CTGGAGAAAC TTTGCTTACT CTCAAAAACC TGACTAGCCT TGATATCAGT 
AAGAATAGTT TTAAGTCTAT GCCTGAAAAT TGCTGGTGGC CAGAAAAGAT GAAATATTTG 
AACTTATCCA GCACACAAAT ACATAGTTTA ACCTATTGTA TTCCCCAGAC ACTGGAAATT 
TTAGATGTTA GCAATAACAA TCTCAATTCA TTTTCTTTGA TGTTACCACA ACTCAGAGAA 
CTTTATATTT CCAGAAATAA GTTGAAGACT CTACCAGATT CCACCTTCTT ACCCATGTTA 
CTAGTCATGA GAATCAGCAG GAACACAATA AATACTTTCT CTAAGGAGCA ATTTGAATCC 
CTTCAAAAAC TGAAAACATT GGAAGCGGGT GGAAACAACT TCATTTGCTC CTGTGAATTC 
CTGTCTTTCA TGCAGGAGCA GCAAGCACTG ACTAAGGTCT TGGTTGACTG GCCAGAAAAC 
TACCTGTGTG ACTCTCCATC CAATGTGCGG GGCCAGCGAG TTCAGGACAC GCGCCTCTCG 
GTTGCTGAGT GCCACAGGGC AGCCCTGGTG TCTGCTGTGT GCTGTGCCCT TCTTCTGTTC 
ATCTTGCTCA TGGGTGTTCT GTGCCACCGT CTCCATGGGC TGTGGTACAT GAAAATGATG 
TGGGCCTGGC TCCAGGCCAA AAGGAAGCCC AAGAAGGCAC ACAACAGGGA CATCTGTTAC 
GACGCTTTTG TTTCTTATAG TGAAAGGGAT TCCTACTGGG TGGAGAGCCT TATGGTCCAG 
GAGCTGGAGA ATTTCGACCC CCCCTTTAGG TTATGTCTTC ATAAGCGGGA CTTTGTTCCT 
GGCAAGTGGA TTATTGACAA TATCATTGAC TGCATTGAAA AGAGTCACAA AACCGTCTTT 
GTGCTTTCTG AAAACTTTGT GAAGAGTGAG TGGTGCAAGT ATGAACTGGA CTTCTCCCAT 
TTTCGTCTCT TTGATGAGAA CAATGATGCT GCCATTCTCA TTCTTCTGGA GCCCATTGAG 
AAGAAAGCCA TTCCCCAGCG GTTCTGTAAA CTGCGGAAGA TCATGAATAC CAAGACCTAC 
CTGGAGTGGC CCAATGATGA AGCTCAGCAG CAAGGGTTTT GGCTAAATTT GAGAACTGCA 
ATAAAGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101342847 ( XM_023733515.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023589283.1 Trichechus manatus latirostris toll like receptor 2 (LOC101342847), mRNA.

Price & Availability↑

CloneID	OTr175898
Clone ID Related Accession (Same CDS sequence)	XM_004378810.2 , XM_023733515.1
Accession Version	XM_023733515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	toll-like receptor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443995.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCATG CTTTGTGGAT ACTGTGGGCC TTGGGGGCCA TAATCACCCT CTCCAAAGGA 
GGGTCCCCTG ATCAGGCTTT TCTGTCTTGT GACCCCACGG GTGTCTGCGA TGGCTCCTCC 
AGGTCTTTGA ACTCCATTCC CTCAGGGCTC ACAGAAGCTG TGAAAAGCCT TGACCTGTCC 
AACAACAAGA TTGCATATGT TGGCAACACT GACCTTCAGT TGTGTGTGAA CCTCAAAGCT 
CTGATTCTGA AGTCCAGTGA AATTAATAGA ATAGATGAAG ATGCTTTTCT TCCCCTTGGA 
AGTCTTGAAC ATTTGGACTT ATCCTATAAT TACTTATCTA ATTTATCATC CTCCTGGTTC 
AGGCCCCTTT CTTCCTTGCA GTTCTTAAGC TTACTGGGAA ATCTTTACAA AGAACTTGGG 
GAAACATCTC TTTTTTCTCA TCTCACAAAT TTGCGAGTCC TGAAAGTAGG AAACATTTAC 
ACCTCTAAGA TTCAGAAAAA GGATTTTACT GGAATAACTT TTCTTAAGGA ACTTGAGATT 
GATGCTTCAA ATCTCCAGAG TTATGAGCCA GAAAGCTTGA AGTCAATTCA GAATATAAGT 
CATCTGATCC TTCATATGAG GCAGCCTATT TTACTGCCGG CGACTTTTGT AGATCTTTTA 
AGTTCCTTGG AATATTTTGA ACTGAGAAAT ACTGATTTGC ACACTTTGCA TTTTCCAGAA 
CTACCCATCA GTGAAACCAA CCCACTGATT AAAAAGTTCA CCTTTAGGAA TGTGGAAATC 
ACTGATGAAA GTTTTAATGA AGTTATGAAA CTGTTCAGTT CTGCCTCTGA ACTTTCAGAA 
TTAGAGTTTG ATGACTGTAC CCTTAATGGA ATCGGTAATT TTGATTCATC TGTTATAGAG 
AAAATTAAAG ATCCAGGTAA AGTAGAAACA TTAATAATAC GGAAGTTGCA TATTCCACAG 
TTTTACTTAT TTAATGATTT GAGTAGTATA TATTCACTCA CAGGAAACCT TAAAAGAATC 
ACAATAGAAA ACAGCAAGGT TTTTCTGGTT CCTTGTTTAC TTTCACAGCA TTTAAAATCA 
TTAGAATACT TGGATCTGAG TGAAAATCTG ATGGTTGAAG AATACTTGAA AAATTCAGCT 
TGTAAGGATG CCTGGCCCTC CCTACAAACC TTAATTTTAA GGCAAAATCA CCTGACATCA 
TTAGAGAAAA CTGGAGAAAC TTTGCTTACT CTCAAAAACC TGACTAGCCT TGATATCAGT 
AAGAATAGTT TTAAGTCTAT GCCTGAAAAT TGCTGGTGGC CAGAAAAGAT GAAATATTTG 
AACTTATCCA GCACACAAAT ACATAGTTTA ACCTATTGTA TTCCCCAGAC ACTGGAAATT 
TTAGATGTTA GCAATAACAA TCTCAATTCA TTTTCTTTGA TGTTACCACA ACTCAGAGAA 
CTTTATATTT CCAGAAATAA GTTGAAGACT CTACCAGATT CCACCTTCTT ACCCATGTTA 
CTAGTCATGA GAATCAGCAG GAACACAATA AATACTTTCT CTAAGGAGCA ATTTGAATCC 
CTTCAAAAAC TGAAAACATT GGAAGCGGGT GGAAACAACT TCATTTGCTC CTGTGAATTC 
CTGTCTTTCA TGCAGGAGCA GCAAGCACTG ACTAAGGTCT TGGTTGACTG GCCAGAAAAC 
TACCTGTGTG ACTCTCCATC CAATGTGCGG GGCCAGCGAG TTCAGGACAC GCGCCTCTCG 
GTTGCTGAGT GCCACAGGGC AGCCCTGGTG TCTGCTGTGT GCTGTGCCCT TCTTCTGTTC 
ATCTTGCTCA TGGGTGTTCT GTGCCACCGT CTCCATGGGC TGTGGTACAT GAAAATGATG 
TGGGCCTGGC TCCAGGCCAA AAGGAAGCCC AAGAAGGCAC ACAACAGGGA CATCTGTTAC 
GACGCTTTTG TTTCTTATAG TGAAAGGGAT TCCTACTGGG TGGAGAGCCT TATGGTCCAG 
GAGCTGGAGA ATTTCGACCC CCCCTTTAGG TTATGTCTTC ATAAGCGGGA CTTTGTTCCT 
GGCAAGTGGA TTATTGACAA TATCATTGAC TGCATTGAAA AGAGTCACAA AACCGTCTTT 
GTGCTTTCTG AAAACTTTGT GAAGAGTGAG TGGTGCAAGT ATGAACTGGA CTTCTCCCAT 
TTTCGTCTCT TTGATGAGAA CAATGATGCT GCCATTCTCA TTCTTCTGGA GCCCATTGAG 
AAGAAAGCCA TTCCCCAGCG GTTCTGTAAA CTGCGGAAGA TCATGAATAC CAAGACCTAC 
CTGGAGTGGC CCAATGATGA AGCTCAGCAG CAAGGGTTTT GGCTAAATTT GAGAACTGCA 
ATAAAGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023589283.1
CDS	103..2454
Translation	MSHALWILWALGAIITLSKGGSPDQAFLSCDPTGVCDGSSRSLNSIPSGLTEAVKSLDLSNNKIAYVGNTDLQLCVNLKALILKSSEINRIDEDAFLPLGSLEHLDLSYNYLSNLSSSWFRPLSSLQFLSLLGNLYKELGETSLFSHLTNLRVLKVGNIYTSKIQKKDFTGITFLKELEIDASNLQSYEPESLKSIQNISHLILHMRQPILLPATFVDLLSSLEYFELRNTDLHTLHFPELPISETNPLIKKFTFRNVEITDESFNEVMKLFSSASELSELEFDDCTLNGIGNFDSSVIEKIKDPGKVETLIIRKLHIPQFYLFNDLSSIYSLTGNLKRITIENSKVFLVPCLLSQHLKSLEYLDLSENLMVEEYLKNSACKDAWPSLQTLILRQNHLTSLEKTGETLLTLKNLTSLDISKNSFKSMPENCWWPEKMKYLNLSSTQIHSLTYCIPQTLEILDVSNNNLNSFSLMLPQLRELYISRNKLKTLPDSTFLPMLLVMRISRNTINTFSKEQFESLQKLKTLEAGGNNFICSCEFLSFMQEQQALTKVLVDWPENYLCDSPSNVRGQRVQDTRLSVAECHRAALVSAVCCALLLFILLMGVLCHRLHGLWYMKMMWAWLQAKRKPKKAHNRDICYDAFVSYSERDSYWVESLMVQELENFDPPFRLCLHKRDFVPGKWIIDNIIDCIEKSHKTVFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPNDEAQQQGFWLNLRTAIKS

Target ORF information:

RefSeq Version	XM_023733515.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris toll like receptor 2 (LOC101342847), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023733515.1

ORF Insert Sequence:

ATGTCGCATG CTTTGTGGAT ACTGTGGGCC TTGGGGGCCA TAATCACCCT CTCCAAAGGA 
GGGTCCCCTG ATCAGGCTTT TCTGTCTTGT GACCCCACGG GTGTCTGCGA TGGCTCCTCC 
AGGTCTTTGA ACTCCATTCC CTCAGGGCTC ACAGAAGCTG TGAAAAGCCT TGACCTGTCC 
AACAACAAGA TTGCATATGT TGGCAACACT GACCTTCAGT TGTGTGTGAA CCTCAAAGCT 
CTGATTCTGA AGTCCAGTGA AATTAATAGA ATAGATGAAG ATGCTTTTCT TCCCCTTGGA 
AGTCTTGAAC ATTTGGACTT ATCCTATAAT TACTTATCTA ATTTATCATC CTCCTGGTTC 
AGGCCCCTTT CTTCCTTGCA GTTCTTAAGC TTACTGGGAA ATCTTTACAA AGAACTTGGG 
GAAACATCTC TTTTTTCTCA TCTCACAAAT TTGCGAGTCC TGAAAGTAGG AAACATTTAC 
ACCTCTAAGA TTCAGAAAAA GGATTTTACT GGAATAACTT TTCTTAAGGA ACTTGAGATT 
GATGCTTCAA ATCTCCAGAG TTATGAGCCA GAAAGCTTGA AGTCAATTCA GAATATAAGT 
CATCTGATCC TTCATATGAG GCAGCCTATT TTACTGCCGG CGACTTTTGT AGATCTTTTA 
AGTTCCTTGG AATATTTTGA ACTGAGAAAT ACTGATTTGC ACACTTTGCA TTTTCCAGAA 
CTACCCATCA GTGAAACCAA CCCACTGATT AAAAAGTTCA CCTTTAGGAA TGTGGAAATC 
ACTGATGAAA GTTTTAATGA AGTTATGAAA CTGTTCAGTT CTGCCTCTGA ACTTTCAGAA 
TTAGAGTTTG ATGACTGTAC CCTTAATGGA ATCGGTAATT TTGATTCATC TGTTATAGAG 
AAAATTAAAG ATCCAGGTAA AGTAGAAACA TTAATAATAC GGAAGTTGCA TATTCCACAG 
TTTTACTTAT TTAATGATTT GAGTAGTATA TATTCACTCA CAGGAAACCT TAAAAGAATC 
ACAATAGAAA ACAGCAAGGT TTTTCTGGTT CCTTGTTTAC TTTCACAGCA TTTAAAATCA 
TTAGAATACT TGGATCTGAG TGAAAATCTG ATGGTTGAAG AATACTTGAA AAATTCAGCT 
TGTAAGGATG CCTGGCCCTC CCTACAAACC TTAATTTTAA GGCAAAATCA CCTGACATCA 
TTAGAGAAAA CTGGAGAAAC TTTGCTTACT CTCAAAAACC TGACTAGCCT TGATATCAGT 
AAGAATAGTT TTAAGTCTAT GCCTGAAAAT TGCTGGTGGC CAGAAAAGAT GAAATATTTG 
AACTTATCCA GCACACAAAT ACATAGTTTA ACCTATTGTA TTCCCCAGAC ACTGGAAATT 
TTAGATGTTA GCAATAACAA TCTCAATTCA TTTTCTTTGA TGTTACCACA ACTCAGAGAA 
CTTTATATTT CCAGAAATAA GTTGAAGACT CTACCAGATT CCACCTTCTT ACCCATGTTA 
CTAGTCATGA GAATCAGCAG GAACACAATA AATACTTTCT CTAAGGAGCA ATTTGAATCC 
CTTCAAAAAC TGAAAACATT GGAAGCGGGT GGAAACAACT TCATTTGCTC CTGTGAATTC 
CTGTCTTTCA TGCAGGAGCA GCAAGCACTG ACTAAGGTCT TGGTTGACTG GCCAGAAAAC 
TACCTGTGTG ACTCTCCATC CAATGTGCGG GGCCAGCGAG TTCAGGACAC GCGCCTCTCG 
GTTGCTGAGT GCCACAGGGC AGCCCTGGTG TCTGCTGTGT GCTGTGCCCT TCTTCTGTTC 
ATCTTGCTCA TGGGTGTTCT GTGCCACCGT CTCCATGGGC TGTGGTACAT GAAAATGATG 
TGGGCCTGGC TCCAGGCCAA AAGGAAGCCC AAGAAGGCAC ACAACAGGGA CATCTGTTAC 
GACGCTTTTG TTTCTTATAG TGAAAGGGAT TCCTACTGGG TGGAGAGCCT TATGGTCCAG 
GAGCTGGAGA ATTTCGACCC CCCCTTTAGG TTATGTCTTC ATAAGCGGGA CTTTGTTCCT 
GGCAAGTGGA TTATTGACAA TATCATTGAC TGCATTGAAA AGAGTCACAA AACCGTCTTT 
GTGCTTTCTG AAAACTTTGT GAAGAGTGAG TGGTGCAAGT ATGAACTGGA CTTCTCCCAT 
TTTCGTCTCT TTGATGAGAA CAATGATGCT GCCATTCTCA TTCTTCTGGA GCCCATTGAG 
AAGAAAGCCA TTCCCCAGCG GTTCTGTAAA CTGCGGAAGA TCATGAATAC CAAGACCTAC 
CTGGAGTGGC CCAATGATGA AGCTCAGCAG CAAGGGTTTT GGCTAAATTT GAGAACTGCA 
ATAAAGTCCT AG