PCNA cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCNA
Entrez Gene ID	692192
Full Name	proliferating cell nuclear antigen
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001291925.1	NP_001278854.1	proliferating cell nuclear antigen

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Intestinal cellular localization of PCNA protein and CYP1A mRNA in Atlantic salmon Salmo salar L. exposed to a model toxicant.
Sanden M, Olsvik PA
BMC physiology93(2009 Mar)

Enterocyte proliferation and apoptosis in the caudal small intestine is influenced by the composition of colonizing commensal bacteria in the neonatal gnotobiotic pig.
Willing BP, Van Kessel AG
Journal of animal science85(12)3256-66(2007 Dec)

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
J?rgensen FG, Hobolth A, Hornsh?j H, Bendixen C, Fredholm M, Schierup MH
BMC biology32(2005 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There is a link between the intestinal detoxification system (CYP1A) & cell renewal system (PCNA). These two processes are inversely correlated in beta-naphthoflavone- exposed fish
Title: Intestinal cellular localization of PCNA protein and CYP1A mRNA in Atlantic salmon Salmo salar L. exposed to a model toxicant.

The following PCNA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCNA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00722	NM_001291925.1 Latest version!	Sus scrofa proliferating cell nuclear antigen (PCNA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCNA ( NM_001291925.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001278854.1 Sus scrofa proliferating cell nuclear antigen (PCNA), mRNA.

Price & Availability↑

CloneID	OSe00722
Clone ID Related Accession (Same CDS sequence)	NM_001291925.1
Accession Version	NM_001291925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-24
Organism	Sus scrofa(pig)
Product	proliferating cell nuclear antigen
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ913657.1. On May 9, 2014 this sequence version replaced XM_003359883.2. ##Evidence-Data-START## Transcript exon combination :: AK239960.1, AY610109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02584787 [ECO:0000348] ##Evidence-Data-END##

ATGTTCGAGG CGCGCCTGGT CCAGGGCTCT ATCCTGAAGA AGGTGCTGGA AGCGCTTAAA 
GACCTCATCA ACGAGGCCTG CTGGGACATC AGCTCCAGCG GCGTGAACCT GCAGAGCATG 
GACTCGTCTC ATGTCTCTTT GGTGCAGCTC ACCCTGCGCT CCGAGGGCTT CGACACGTAC 
CGCTGCGACC GCAATTTGGC CATGGGCGTG AACCTCACCA GCATGTCCAA AATACTAAAA 
TGTGCTGGCA ATGAAGACAT CATTACGCTA AGGGCAGAAG ATAATGCAGA CACCTTGGCA 
CTAGTGTTTG AAGCTCCAAA TCAGGAAAAG GTTTCAGACT ATGAAATGAA GTTAATGGAT 
TTAGATGTTG AGCAACTTGG AATTCCAGAA CAAGAGTACA GCTGTGTAGT AAAGATGCCT 
TCTGGTGAAT TTGCACGTAT ATGCCGAGAT CTCAGTCACA TTGGAGATGC TGTTGTAATT 
TCCTGTGCAA AAGATGGAGT GAAATTTTCT GCAAGTGGAG AACTCGGAAA TGGAAATATT 
AAGTTGTCAC AAACGAGTAA TGTTGATAAA GAGGAGGAAG CAGTTACCAT AGAGATGAAT 
GAACCAGTTC AGCTAACTTT TGCACTGAGG TACCTGAACT TCTTTACAAA AGCCACTCCA 
CTCTCTCCTA CAGTCACACT CAGTATGTCT GCAGATGTAC CCCTTGTTGT AGAGTATAAA 
ATTGCTGATA TGGGACATTT AAAGTACTAT TTGGCTCCCA AGATCGAAGA TGAAGAAGGG 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278854.1
CDS	42..827
Translation	MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSPTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS

Target ORF information:

RefSeq Version	NM_001291925.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa proliferating cell nuclear antigen (PCNA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291925.1

ORF Insert Sequence:

ATGTTCGAGG CGCGCCTGGT CCAGGGCTCT ATCCTGAAGA AGGTGCTGGA AGCGCTTAAA 
GACCTCATCA ACGAGGCCTG CTGGGACATC AGCTCCAGCG GCGTGAACCT GCAGAGCATG 
GACTCGTCTC ATGTCTCTTT GGTGCAGCTC ACCCTGCGCT CCGAGGGCTT CGACACGTAC 
CGCTGCGACC GCAATTTGGC CATGGGCGTG AACCTCACCA GCATGTCCAA AATACTAAAA 
TGTGCTGGCA ATGAAGACAT CATTACGCTA AGGGCAGAAG ATAATGCAGA CACCTTGGCA 
CTAGTGTTTG AAGCTCCAAA TCAGGAAAAG GTTTCAGACT ATGAAATGAA GTTAATGGAT 
TTAGATGTTG AGCAACTTGG AATTCCAGAA CAAGAGTACA GCTGTGTAGT AAAGATGCCT 
TCTGGTGAAT TTGCACGTAT ATGCCGAGAT CTCAGTCACA TTGGAGATGC TGTTGTAATT 
TCCTGTGCAA AAGATGGAGT GAAATTTTCT GCAAGTGGAG AACTCGGAAA TGGAAATATT 
AAGTTGTCAC AAACGAGTAA TGTTGATAAA GAGGAGGAAG CAGTTACCAT AGAGATGAAT 
GAACCAGTTC AGCTAACTTT TGCACTGAGG TACCTGAACT TCTTTACAAA AGCCACTCCA 
CTCTCTCCTA CAGTCACACT CAGTATGTCT GCAGATGTAC CCCTTGTTGT AGAGTATAAA 
ATTGCTGATA TGGGACATTT AAAGTACTAT TTGGCTCCCA AGATCGAAGA TGAAGAAGGG 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intestinal cellular localization of PCNA protein and CYP1A mRNA in Atlantic salmon Salmo salar L. exposed to a model toxicant.
BMC physiology93(2009 Mar)
Sanden M,Olsvik PA

Enterocyte proliferation and apoptosis in the caudal small intestine is influenced by the composition of colonizing commensal bacteria in the neonatal gnotobiotic pig.
Journal of animal science85(12)3256-66(2007 Dec)
Willing BP,Van Kessel AG

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
BMC biology32(2005 Jan)
J?rgensen FG,Hobolth A,Hornsh?j H,Bendixen C,Fredholm M,Schierup MH

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T