PHKG1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHKG1
Entrez Gene ID	397548
Full Name	phosphorylase kinase catalytic subunit gamma 1
Synonyms	PHGK
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 3 Map Location: 3\|3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001293144.1	NP_001280073.1	phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A splice mutation in the PHKG1 gene causes high glycogen content and low meat quality in pig skeletal muscle.
Ma J, Yang J, Zhou L, Ren J, Liu X, Zhang H, Yang B, Zhang Z, Ma H, Xie X, Xing Y, Guo Y, Huang L
PLoS genetics10(10)e1004710(2014 Oct)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Detection and mapping of mutations in the porcine phosphoglucomutase 1 (PGM1) and phosphorylase kinase gamma unit (PHKG) genes using F-SSCP-analysis.
Looft C, Paul S, Kalm E
Animal genetics30(3)234(1999 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deep sequencing of PHKG1 revealed a point mutation (C>A) in a splice acceptor site of intron 9, resulting in a 32-bp deletion in the open reading frame and generating a premature stop codon.
Title: A splice mutation in the PHKG1 gene causes high glycogen content and low meat quality in pig skeletal muscle.

The following PHKG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00823	NM_001293144.1 Latest version!	Sus scrofa phosphorylase kinase catalytic subunit gamma 1 (PHKG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHKG1 ( NM_001293144.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001280073.1 Sus scrofa phosphorylase kinase catalytic subunit gamma 1 (PHKG1), mRNA.

Price & Availability↑

CloneID	OSe00823
Clone ID Related Accession (Same CDS sequence)	NM_001293144.1
Accession Version	NM_001293144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Sus scrofa(pig)
Product	phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KJ481911.1. On May 30, 2014 this sequence version replaced XM_003124459.3. ##Evidence-Data-START## Transcript exon combination :: AK234617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END##

ATGACCAAGG ACGCGGCACT GCCGGAATCC CACTCAGCAC AGGGCTTCTA TGAGAACTAT 
GAGCCCAAGG AGATCCTGGG CAGGGGAGTG AGCAGTGTGG TCCGGCGCTG CATCCACAAG 
CCCACGTGCC AGGAGTACGC GGTGAAGATC ATTGACATCA CGGGCGGGGG CAGCTTCAGC 
TCCGAGGAGG TGCAGGAGCT GCGAGAGGCC ACGCTGAAGG AGGTGGACAT CCTGCGCAAG 
GTCTCAGGCC ACCCCAACAT CATACAGCTC AAGGACACTT ATGAGACCAA CACTTTCTTC 
TTCCTGGTGT TTGATCTGAT GAAGAGAGGG GAGCTCTTTG ATTACCTCAC TGAGAAGGTC 
ACCCTGAGTG AGAAGGAAAC CAGGAAGATC ATGAGAGCCC TGCTGGAGGT GATCTGCGCC 
TTGCACAAGC TCGACATCGT GCATCGGGAT CTGAAGCCTG AGAACATCCT CTTGGACGAC 
AACATGAACA TCAAGCTCAC GGACTTTGGC TTTTCCTGCC AGCTGAAGCC AGGAGAGAAG 
CTGCGAGAGG TCTGTGGGAC CCCCAGTTAC CTGGCCCCCG AGATCATCGA GTGCTCCATG 
AACGATGACC ACCCAGGCTA CGGGAAGGAG GTGGACATGT GGAGCACAGG GGTCATCATG 
TACACCCTGC TGGCCGGCTC CCCGCCCTTC TGGCACCGCA AGCAGATGCT GATGCTGAGG 
ATGATCATGA GCGGCCACTA CCAGTTCGGC TCACCGGAAT GGGATGATTA CTCGGACACC 
GTGAAGGACC TGGTCTCCCG CTTCTTGGTG GTGAACCCCC AGAGCCGCTG TTCGGCAGAA 
GAGGCCTTAG CGCACCCCTT TTTCCAGCAG TACGTGGTGG AGGAAGTGCG TCACTTCAGC 
CCCCGGGGCA AGTTCAAGGT GATCGCTCTG ACGGTGCTGG CTTCTGTGAG GATCTATTAC 
CAGTACCGCC GGGTGAAGCC TGTGACCCGG GAGATCGTCA TCCGAGACCC CTACGCCCTC 
CGGCCTCTGC GGCGACTCAT CGATGCCTAC GCATTCCGAA TCTATGGCCA CTGGGTGAAG 
AAGGGGCAGC AGCAGAACCG GGCAGCCCTC TTCGAGAACA CACCCAAGGC CGTGCTCCTC 
TCGCTGGCTG AGGACGAGGA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280073.1
CDS	157..1323
Translation	MTKDAALPESHSAQGFYENYEPKEILGRGVSSVVRRCIHKPTCQEYAVKIIDITGGGSFSSEEVQELREATLKEVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLSEKETRKIMRALLEVICALHKLDIVHRDLKPENILLDDNMNIKLTDFGFSCQLKPGEKLREVCGTPSYLAPEIIECSMNDDHPGYGKEVDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMSGHYQFGSPEWDDYSDTVKDLVSRFLVVNPQSRCSAEEALAHPFFQQYVVEEVRHFSPRGKFKVIALTVLASVRIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAYAFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEDEDY

Target ORF information:

RefSeq Version	NM_001293144.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa phosphorylase kinase catalytic subunit gamma 1 (PHKG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293144.1

ORF Insert Sequence:

ATGACCAAGG ACGCGGCACT GCCGGAATCC CACTCAGCAC AGGGCTTCTA TGAGAACTAT 
GAGCCCAAGG AGATCCTGGG CAGGGGAGTG AGCAGTGTGG TCCGGCGCTG CATCCACAAG 
CCCACGTGCC AGGAGTACGC GGTGAAGATC ATTGACATCA CGGGCGGGGG CAGCTTCAGC 
TCCGAGGAGG TGCAGGAGCT GCGAGAGGCC ACGCTGAAGG AGGTGGACAT CCTGCGCAAG 
GTCTCAGGCC ACCCCAACAT CATACAGCTC AAGGACACTT ATGAGACCAA CACTTTCTTC 
TTCCTGGTGT TTGATCTGAT GAAGAGAGGG GAGCTCTTTG ATTACCTCAC TGAGAAGGTC 
ACCCTGAGTG AGAAGGAAAC CAGGAAGATC ATGAGAGCCC TGCTGGAGGT GATCTGCGCC 
TTGCACAAGC TCGACATCGT GCATCGGGAT CTGAAGCCTG AGAACATCCT CTTGGACGAC 
AACATGAACA TCAAGCTCAC GGACTTTGGC TTTTCCTGCC AGCTGAAGCC AGGAGAGAAG 
CTGCGAGAGG TCTGTGGGAC CCCCAGTTAC CTGGCCCCCG AGATCATCGA GTGCTCCATG 
AACGATGACC ACCCAGGCTA CGGGAAGGAG GTGGACATGT GGAGCACAGG GGTCATCATG 
TACACCCTGC TGGCCGGCTC CCCGCCCTTC TGGCACCGCA AGCAGATGCT GATGCTGAGG 
ATGATCATGA GCGGCCACTA CCAGTTCGGC TCACCGGAAT GGGATGATTA CTCGGACACC 
GTGAAGGACC TGGTCTCCCG CTTCTTGGTG GTGAACCCCC AGAGCCGCTG TTCGGCAGAA 
GAGGCCTTAG CGCACCCCTT TTTCCAGCAG TACGTGGTGG AGGAAGTGCG TCACTTCAGC 
CCCCGGGGCA AGTTCAAGGT GATCGCTCTG ACGGTGCTGG CTTCTGTGAG GATCTATTAC 
CAGTACCGCC GGGTGAAGCC TGTGACCCGG GAGATCGTCA TCCGAGACCC CTACGCCCTC 
CGGCCTCTGC GGCGACTCAT CGATGCCTAC GCATTCCGAA TCTATGGCCA CTGGGTGAAG 
AAGGGGCAGC AGCAGAACCG GGCAGCCCTC TTCGAGAACA CACCCAAGGC CGTGCTCCTC 
TCGCTGGCTG AGGACGAGGA CTACTGA