GRB2 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRB2
Entrez Gene ID	100192436
Full Name	growth factor receptor bound protein 2
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 12 Map Location: 12\|12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001137628.1	NP_001131100.1	growth factor receptor-bound protein 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Insulin-like growth factor-I stimulates Shc-dependent phosphatidylinositol 3-kinase activation via Grb2-associated p85 in vascular smooth muscle cells.
Radhakrishnan Y, Maile LA, Ling Y, Graves LM, Clemmons DR
The Journal of biological chemistry283(24)16320-31(2008 Jun)

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
J?rgensen FG, Hobolth A, Hornsh?j H, Bendixen C, Fredholm M, Schierup MH
BMC biology32(2005 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in VSMCs exposed to hyperglycemia, IGF-I stimulation of Shc facilitates the transfer of Grb2 to p85 resulting in enhanced PI3K activation and AKT phosphorylation leading to enhanced cell proliferation and migration
Title: Insulin-like growth factor-I stimulates Shc-dependent phosphatidylinositol 3-kinase activation via Grb2-associated p85 in vascular smooth muscle cells.

The following GRB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe29765	NM_001137628.1 Latest version!	Sus scrofa growth factor receptor bound protein 2 (GRB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRB2 ( NM_001137628.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001131100.1 Sus scrofa growth factor receptor bound protein 2 (GRB2), mRNA.

Price & Availability↑

CloneID	OSe29765
Clone ID Related Accession (Same CDS sequence)	NM_001137628.1
Accession Version	NM_001137628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-08
Organism	Sus scrofa(pig)
Product	growth factor receptor-bound protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ196595.1. ##Evidence-Data-START## Transcript exon combination :: FJ196595.1, BP142256.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3180021, SAMN01048242 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCCA TCGCCAAGTA TGACTTCAAA GCCACCGCAG ACGACGAGCT GAGCTTCAAA 
AGGGGGGACA TCCTCAAGGT TTTGAATGAA GAATGTGATC AGAACTGGTA CAAAGCAGAG 
CTCAATGGGA AAGATGGCTT CATCCCCAAG AACTACATAG AAATGAAACC ACACCCGTGG 
TTTTTTGGCA AAATCCCCAG AGCCAAGGCA GAAGAAATGC TTAGCAAACA GCGGCACGAT 
GGGGCCTTTC TCATCCGTGA GAGCGAGAGC GCTCCTGGGG ATTTCTCCCT CTCTGTCAAG 
TTTGGCAATG ATGTGCAGCA CTTCAAGGTG CTCCGGGACG GCGCTGGCAA GTACTTCCTC 
TGGGTGGTGA AGTTCAATTC TTTGAATGAG CTGGTAGATT ATCACAGATC TACATCCGTC 
TCCAGAAACC AGCAGATATT CCTCCGGGAC ATAGAACAGG TGCCACAGCA ACCGACATAC 
GTCCAGGCCC TCTTTGACTT TGACCCCCAG GAGGATGGTG AGCTGGGCTT CCGTCGGGGA 
GACTTTATCC ACGTCATGGA TAACTCGGAC CCCAACTGGT GGAAGGGGGC TTGCCACGGG 
CAGACCGGCA TGTTTCCGCG CAATTACGTC ACCCCTGTGA ACCGGAACGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131100.1
CDS	272..925
Translation	MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Target ORF information:

RefSeq Version	NM_001137628.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa growth factor receptor bound protein 2 (GRB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137628.1

ORF Insert Sequence:

ATGGAAGCCA TCGCCAAGTA TGACTTCAAA GCCACCGCAG ACGACGAGCT GAGCTTCAAA 
AGGGGGGACA TCCTCAAGGT TTTGAATGAA GAATGTGATC AGAACTGGTA CAAAGCAGAG 
CTCAATGGGA AAGATGGCTT CATCCCCAAG AACTACATAG AAATGAAACC ACACCCGTGG 
TTTTTTGGCA AAATCCCCAG AGCCAAGGCA GAAGAAATGC TTAGCAAACA GCGGCACGAT 
GGGGCCTTTC TCATCCGTGA GAGCGAGAGC GCTCCTGGGG ATTTCTCCCT CTCTGTCAAG 
TTTGGCAATG ATGTGCAGCA CTTCAAGGTG CTCCGGGACG GCGCTGGCAA GTACTTCCTC 
TGGGTGGTGA AGTTCAATTC TTTGAATGAG CTGGTAGATT ATCACAGATC TACATCCGTC 
TCCAGAAACC AGCAGATATT CCTCCGGGAC ATAGAACAGG TGCCACAGCA ACCGACATAC 
GTCCAGGCCC TCTTTGACTT TGACCCCCAG GAGGATGGTG AGCTGGGCTT CCGTCGGGGA 
GACTTTATCC ACGTCATGGA TAACTCGGAC CCCAACTGGT GGAAGGGGGC TTGCCACGGG 
CAGACCGGCA TGTTTCCGCG CAATTACGTC ACCCCTGTGA ACCGGAACGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insulin-like growth factor-I stimulates Shc-dependent phosphatidylinositol 3-kinase activation via Grb2-associated p85 in vascular smooth muscle cells.
The Journal of biological chemistry283(24)16320-31(2008 Jun)
Radhakrishnan Y,Maile LA,Ling Y,Graves LM,Clemmons DR

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
BMC biology32(2005 Jan)
J?rgensen FG,Hobolth A,Hornsh?j H,Bendixen C,Fredholm M,Schierup MH

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T