WEE1 cDNA ORF clone, Struthio camelus australis

Gene
Clones
gRNAs

Summary

Gene Symbol	WEE1
Entrez Gene ID	104147533
Full Name	WEE1 G2 checkpoint kinase
Gene Type	protein-coding
Organism	Struthio camelus australis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009680254.1	XP_009678549.1	wee1-like protein kinase

Pathways from BioSystems
Gene Ontology

The following WEE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WEE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt77132	XM_009680254.1 Latest version!	Struthio camelus australis WEE1 G2 checkpoint kinase (WEE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WEE1 ( XM_009680254.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009678549.1 Struthio camelus australis WEE1 G2 checkpoint kinase (WEE1), mRNA.

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CloneID	OSt77132
Clone ID Related Accession (Same CDS sequence)	XM_009680254.1
Accession Version	XM_009680254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	wee1-like protein kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009271530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 14% of CDS bases ##RefSeq-Attributes-END##

ATGGGCTCCA GCTCAGTTAA ACTTCGAGGT GGCTCTCTAT TTATGAATGT TGGGAAATCT 
GAGAAGCAAG ATCCTGATAT TAGACAAACA CCTCATGTGA ACATTAATCC CTTCACTCCC 
GATTCCATGT TCCTTCACAA CTCTGAAGGA CAAGTTCGTC GGAGAAAGAG AACTCACTGG 
AATGACTCTT GTGGAGAGGA CATGGAAGCA AGTGATGGAG AACTTGAAGA TGAAACTGTC 
AGACCTGCTA AGAGGATCAC AATAACAGAA AGTAATATGA AGTCACGCTA TGCAACTGAA 
TTCCACGAGT TGGAGAAGAT CGGATCTGGT GAATTCGGTT CTGTGTTTAA ATGTGTGAAA 
AGGCTTGATG GCTGTATTTA TGCCATAAAG CGCTCCAAAA AACCCTTAGC TGGCTCAGTG 
GATGAGCAAA ATGCTTTAAG AGAGGTCTAT GCACATGCAG TTCTGGGACA GCATTCTCAT 
GTAGTAAGAT ACTACTCTGC ATGGGCAGAA GACGATCATA TGCTTATACA GAACGAGTAT 
TGTAATGGTG GCAGTTTAGC AGATGCCATA AGTGAAAATT ACAGGAATAT GCGTTATTTT 
ACTGAACCAG AGCTGAAAGA CCTACTACTT CAGGTGGCTC GAGGCTTAAA GTACATTCAT 
TCGATGTCAC TTGTACACAT GGATATCAAA CCTAGTAATA TTTTCATATC TAGGACGTCA 
GTCCCAAGTA CGACTTCAGA AGAAGGTGAT GATGATGACT GGTCGTCCGA TAGAGTTATA 
TTTAAAATAG GTGATCTTGG TCATGTAACT CGAGTGTCTA GTCCACAAGT GGAGGAGGGC 
GATAGCCGTT TCCTTGCAAA TGAAGTCCTG CAAGAGAACT ACACTCACTT GCCGAAAGCG 
GATATTTTTG CTCTTGCTCT GACTGTTGTC TGTGCTGCTG GTGCTGAACC ACTTCCAACT 
AATGGGGACC AGTGGCATGA AATTCGACAA GGAAAACTTC CTAGAATACC ACAAGTGCTT 
TCTCAAGAAT TACTAGACTT ACTAAAAATT ATGATTAACC CTGATCCGGA GAAAAGGCCT 
TCAGCTGTGG CACTAGTCAA GCATTCAGTG CTACTGTCTG CCGCAAAAAA GAGCGCAGAG 
CAGCTGCGGA TAGAACTGAA TGCAGAAAAA TTCAAAAACT CTCTCTTGCA GAAAGAGCTG 
AAGAAGGCAC AAATGGCAAA GGCCGCAGCT GAGGAGCGAG CACTGTTCAC TGACAGAATG 
ACAACTAGAT CTTTCCGAGT CCTATCAGCT CACTGTGATT ATCCCTTTAA ATCTCTTGGG 
TACAAGTTGT TGGTTACCGC AGACCTACCT GAAAACTTCA ACGTTGCTGT TTTGACAACT 
AAGAACCTTG CAGAGGGAAA GGGAGAAACA GTGATTGGCC AGGCCAGCAC ACAGGAACTT 
AAGGCCCTGT TCAGGTTTGA ACAGTTGGAA CTGGATGAAG ATGCTACCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009678549.1
CDS	25..1518
Translation	MGSSSVKLRGGSLFMNVGKSEKQDPDIRQTPHVNINPFTPDSMFLHNSEGQVRRRKRTHWNDSCGEDMEASDGELEDETVRPAKRITITESNMKSRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYYSAWAEDDHMLIQNEYCNGGSLADAISENYRNMRYFTEPELKDLLLQVARGLKYIHSMSLVHMDIKPSNIFISRTSVPSTTSEEGDDDDWSSDRVIFKIGDLGHVTRVSSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPTNGDQWHEIRQGKLPRIPQVLSQELLDLLKIMINPDPEKRPSAVALVKHSVLLSAAKKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMTTRSFRVLSAHCDYPFKSLGYKLLVTADLPENFNVAVLTTKNLAEGKGETVIGQASTQELKALFRFEQLELDEDATP

Target ORF information:

RefSeq Version	XM_009680254.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis WEE1 G2 checkpoint kinase (WEE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009680254.1

ORF Insert Sequence:

ATGGGCTCCA GCTCAGTTAA ACTTCGAGGT GGCTCTCTAT TTATGAATGT TGGGAAATCT 
GAGAAGCAAG ATCCTGATAT TAGACAAACA CCTCATGTGA ACATTAATCC CTTCACTCCC 
GATTCCATGT TCCTTCACAA CTCTGAAGGA CAAGTTCGTC GGAGAAAGAG AACTCACTGG 
AATGACTCTT GTGGAGAGGA CATGGAAGCA AGTGATGGAG AACTTGAAGA TGAAACTGTC 
AGACCTGCTA AGAGGATCAC AATAACAGAA AGTAATATGA AGTCACGCTA TGCAACTGAA 
TTCCACGAGT TGGAGAAGAT CGGATCTGGT GAATTCGGTT CTGTGTTTAA ATGTGTGAAA 
AGGCTTGATG GCTGTATTTA TGCCATAAAG CGCTCCAAAA AACCCTTAGC TGGCTCAGTG 
GATGAGCAAA ATGCTTTAAG AGAGGTCTAT GCACATGCAG TTCTGGGACA GCATTCTCAT 
GTAGTAAGAT ACTACTCTGC ATGGGCAGAA GACGATCATA TGCTTATACA GAACGAGTAT 
TGTAATGGTG GCAGTTTAGC AGATGCCATA AGTGAAAATT ACAGGAATAT GCGTTATTTT 
ACTGAACCAG AGCTGAAAGA CCTACTACTT CAGGTGGCTC GAGGCTTAAA GTACATTCAT 
TCGATGTCAC TTGTACACAT GGATATCAAA CCTAGTAATA TTTTCATATC TAGGACGTCA 
GTCCCAAGTA CGACTTCAGA AGAAGGTGAT GATGATGACT GGTCGTCCGA TAGAGTTATA 
TTTAAAATAG GTGATCTTGG TCATGTAACT CGAGTGTCTA GTCCACAAGT GGAGGAGGGC 
GATAGCCGTT TCCTTGCAAA TGAAGTCCTG CAAGAGAACT ACACTCACTT GCCGAAAGCG 
GATATTTTTG CTCTTGCTCT GACTGTTGTC TGTGCTGCTG GTGCTGAACC ACTTCCAACT 
AATGGGGACC AGTGGCATGA AATTCGACAA GGAAAACTTC CTAGAATACC ACAAGTGCTT 
TCTCAAGAAT TACTAGACTT ACTAAAAATT ATGATTAACC CTGATCCGGA GAAAAGGCCT 
TCAGCTGTGG CACTAGTCAA GCATTCAGTG CTACTGTCTG CCGCAAAAAA GAGCGCAGAG 
CAGCTGCGGA TAGAACTGAA TGCAGAAAAA TTCAAAAACT CTCTCTTGCA GAAAGAGCTG 
AAGAAGGCAC AAATGGCAAA GGCCGCAGCT GAGGAGCGAG CACTGTTCAC TGACAGAATG 
ACAACTAGAT CTTTCCGAGT CCTATCAGCT CACTGTGATT ATCCCTTTAA ATCTCTTGGG 
TACAAGTTGT TGGTTACCGC AGACCTACCT GAAAACTTCA ACGTTGCTGT TTTGACAACT 
AAGAACCTTG CAGAGGGAAA GGGAGAAACA GTGATTGGCC AGGCCAGCAC ACAGGAACTT 
AAGGCCCTGT TCAGGTTTGA ACAGTTGGAA CTGGATGAAG ATGCTACCCC CTGA