RFWD3 cDNA ORF clone, Struthio camelus australis

Gene
Clones
gRNAs

Summary

Gene Symbol	RFWD3
Entrez Gene ID	104147114
Full Name	ring finger and WD repeat domain 3
Gene Type	protein-coding
Organism	Struthio camelus australis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009679527.1	XP_009677822.1	E3 ubiquitin-protein ligase RFWD3

Pathways from BioSystems
Gene Ontology

The following RFWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt64795	XM_009679527.1 Latest version!	Struthio camelus australis ring finger and WD repeat domain 3 (RFWD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFWD3 ( XM_009679527.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009677822.1 Struthio camelus australis ring finger and WD repeat domain 3 (RFWD3), mRNA.

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CloneID	OSt64795
Clone ID Related Accession (Same CDS sequence)	XM_009679527.1
Accession Version	XM_009679527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	E3 ubiquitin-protein ligase RFWD3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009270252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAAG AAGAGATGGA AGTTGATCTG CAACCAGTGG CTGGTTACCA CGTAGAGAAC 
CACCGGGCGC TTCTCTCAGA GCCCTCTCAT CACAGAGTAC CACATACTGC AGCGCACAGT 
GAAGAAGGAG ATGTTGTTAC AGTTCTTCCT GCTGATGGAG CTGTTGTGAT ATCAAACGCT 
GATACTACAA GGACTGCTGC TGACTCTGAC CAGCGGTTGG GTTCTGCCTC GGCTCTCCGT 
GGACTGCAAA GGCTACGGGG AGCATTTGAT CAGCATCAGG TATTGCCTGG GCTGCAGATC 
ACGCAGCGTC CGCAAGTAAG AAGAGCTCGC AGGCAACAAA GAATTGGTGG TTCCCAGAGG 
ACAGTCAGTA GCAGGGCAGC ATTGGACAGT TACTTTCAAA TCAGCAGGAC TCAAGAGCCA 
GCTGCAGTAT CAGTTCCACA TCTGGAGCCT TCGCACATTC CAGGAGACAA CCAGGAACAC 
AGCTCAGATG AAACGATAGA AGTGAGTGAC TCTGACAGCA GCACTTCTGA GGAGGAGGAA 
GAGGTGGATG CTGGAGCTCC ACTGGTACCA TCAGCCCAGG AGACACCTGC GCCAGCTAGC 
TTAGCAGTTT CCAATCAGGG CCAAGCAGAC CCACTTCCAG CTCTGCCTTC CTCTGCAGAA 
CATGGAAGTC ATGAAGGTGA AGAGGCTGAC ATCCAGCGAA AGCAGAGAAC TTCACCAAAG 
AAACTGGAAC CATTGGTTCC TGTTGCACCC TTGGATGAGG AAGAAGGTGA TACCTGCGCC 
ATCTGCTTTG AGCAGTGGAC CAATGCTGGG GGCCACCGTC TCTCCGCATT GCGGTGTGGA 
CACCTATTTG GATACACCTG CATTGAAAGG TGGCTCAAGG GACAGGCAGG AAAGTGCCCT 
CAGTGCAATA AGAAAGCAAA ACGTTCGGAC ATTGTGATCC TGTATGCTCG AACTTTGAAA 
GCGCTGGATA CCAGTGAACA GGAACGCATG AAAAGTTCTT TAGAAAAGGA GCAAATGTTG 
CGGAGACAGG CTGAGCTGGA ATCTGCACAG AGCCGTCTCC AGTTGCAGGT TTTGACAGAT 
GAATGCAGCA AACTCCGCAA GCAAGTTCAG GAGTTGAAGG CTTTGGTGGC CCAACACAGC 
GTGACTGCTT CTCAGCAGCC TGGCAGCTCC CGCACCTGCT TCCCAGGTGG TCTCCCCTCC 
AGTCAAAGCC AGCACAAGTA CCACTTTGAG AAGGCCTTTG TGGTGTCTCA GGCTGGAAGC 
TGCAGGGTTA TGGCATACTG TGACTCACTG AGCTGTCTCG TGGTATCACA GCCTTCTCCA 
CAGTCAACTT TTATTCCTGG TTGTGGTGTT AAGATGATGA GCGCGGCCAA CCTGAAGAGC 
AGTCAGTACA TCCCTATCCA TAGTAAACAG ATTCGGGGAC TGGCTTTTGG CAGTCGAGCA 
GATGGCTTGC TGCTGTCAGC TGCTCTGGAC AACACTCTCA AATTAACCAG CTTGGCAACA 
AACACTGTGG TACAGACATA CAATACTGGT CGTCCTGTCT GGAGTTGCTG CTGGTGTCTT 
GATGATACAA ACTACATCTA TGCTGGGCTG GTTAATGGCT CTATCATGGT ATATGATTTG 
AGAGAGACAA GCTCTCACGT CCAGGAGCTG GCCCCTCAGA AGTCCAGGTG CCCCATGGTG 
TCACTGTCCT ACCTGCCACG CATGGCCTCA GCATCATTGC CTTATGGTGG AATAGTAGCT 
GGGACCTTGG AAGGAGCCTG CTTTTGGGAA CAGAAAGCTG GCACCTCTTA CCGGCCTCAT 
CACTTGCCAC TGGAACCTGG AGGATGCATT GATATTCAAA CGGAGATCAA CACACGGCAC 
TGCCTTGCAA CATACAGGCC TAGTAAAAAT AATCCTTGCC TGCGTTGCGT GATGATGGAA 
CTGACCTGCA GCCCACTGAC AGAAGCCTCT GAAGATGTGG TGTGCTCCTC TAACCCAGTT 
CAGACTTTCA GTGCTGGGCC CACTTGCAAG TTGTTGACCA AAAATGCCAT TTTTCAGAGC 
CCAGAGGAGG ATGGCAGTAT TCTTGTGTGT GCTGGCGATG AGGCCTCTAA TTCTGCCATG 
TTATGGGATG CTGGAAGTGG CACCTTACTG CAGAAGCTGC AGGCTGACCT ACCTGTGTTG 
GATATCTGCC CCTTTGAAGT GAACCAAACC CACCTCCTGG CTACGCTCAC TGAGAAAATG 
GTCAAGATCT ACAAGTGGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009677822.1
CDS	142..2385
Translation	MAQEEMEVDLQPVAGYHVENHRALLSEPSHHRVPHTAAHSEEGDVVTVLPADGAVVISNADTTRTAADSDQRLGSASALRGLQRLRGAFDQHQVLPGLQITQRPQVRRARRQQRIGGSQRTVSSRAALDSYFQISRTQEPAAVSVPHLEPSHIPGDNQEHSSDETIEVSDSDSSTSEEEEEVDAGAPLVPSAQETPAPASLAVSNQGQADPLPALPSSAEHGSHEGEEADIQRKQRTSPKKLEPLVPVAPLDEEEGDTCAICFEQWTNAGGHRLSALRCGHLFGYTCIERWLKGQAGKCPQCNKKAKRSDIVILYARTLKALDTSEQERMKSSLEKEQMLRRQAELESAQSRLQLQVLTDECSKLRKQVQELKALVAQHSVTASQQPGSSRTCFPGGLPSSQSQHKYHFEKAFVVSQAGSCRVMAYCDSLSCLVVSQPSPQSTFIPGCGVKMMSAANLKSSQYIPIHSKQIRGLAFGSRADGLLLSAALDNTLKLTSLATNTVVQTYNTGRPVWSCCWCLDDTNYIYAGLVNGSIMVYDLRETSSHVQELAPQKSRCPMVSLSYLPRMASASLPYGGIVAGTLEGACFWEQKAGTSYRPHHLPLEPGGCIDIQTEINTRHCLATYRPSKNNPCLRCVMMELTCSPLTEASEDVVCSSNPVQTFSAGPTCKLLTKNAIFQSPEEDGSILVCAGDEASNSAMLWDAGSGTLLQKLQADLPVLDICPFEVNQTHLLATLTEKMVKIYKWQ

Target ORF information:

RefSeq Version	XM_009679527.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis ring finger and WD repeat domain 3 (RFWD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009679527.1

ORF Insert Sequence:

ATGGCTCAAG AAGAGATGGA AGTTGATCTG CAACCAGTGG CTGGTTACCA CGTAGAGAAC 
CACCGGGCGC TTCTCTCAGA GCCCTCTCAT CACAGAGTAC CACATACTGC AGCGCACAGT 
GAAGAAGGAG ATGTTGTTAC AGTTCTTCCT GCTGATGGAG CTGTTGTGAT ATCAAACGCT 
GATACTACAA GGACTGCTGC TGACTCTGAC CAGCGGTTGG GTTCTGCCTC GGCTCTCCGT 
GGACTGCAAA GGCTACGGGG AGCATTTGAT CAGCATCAGG TATTGCCTGG GCTGCAGATC 
ACGCAGCGTC CGCAAGTAAG AAGAGCTCGC AGGCAACAAA GAATTGGTGG TTCCCAGAGG 
ACAGTCAGTA GCAGGGCAGC ATTGGACAGT TACTTTCAAA TCAGCAGGAC TCAAGAGCCA 
GCTGCAGTAT CAGTTCCACA TCTGGAGCCT TCGCACATTC CAGGAGACAA CCAGGAACAC 
AGCTCAGATG AAACGATAGA AGTGAGTGAC TCTGACAGCA GCACTTCTGA GGAGGAGGAA 
GAGGTGGATG CTGGAGCTCC ACTGGTACCA TCAGCCCAGG AGACACCTGC GCCAGCTAGC 
TTAGCAGTTT CCAATCAGGG CCAAGCAGAC CCACTTCCAG CTCTGCCTTC CTCTGCAGAA 
CATGGAAGTC ATGAAGGTGA AGAGGCTGAC ATCCAGCGAA AGCAGAGAAC TTCACCAAAG 
AAACTGGAAC CATTGGTTCC TGTTGCACCC TTGGATGAGG AAGAAGGTGA TACCTGCGCC 
ATCTGCTTTG AGCAGTGGAC CAATGCTGGG GGCCACCGTC TCTCCGCATT GCGGTGTGGA 
CACCTATTTG GATACACCTG CATTGAAAGG TGGCTCAAGG GACAGGCAGG AAAGTGCCCT 
CAGTGCAATA AGAAAGCAAA ACGTTCGGAC ATTGTGATCC TGTATGCTCG AACTTTGAAA 
GCGCTGGATA CCAGTGAACA GGAACGCATG AAAAGTTCTT TAGAAAAGGA GCAAATGTTG 
CGGAGACAGG CTGAGCTGGA ATCTGCACAG AGCCGTCTCC AGTTGCAGGT TTTGACAGAT 
GAATGCAGCA AACTCCGCAA GCAAGTTCAG GAGTTGAAGG CTTTGGTGGC CCAACACAGC 
GTGACTGCTT CTCAGCAGCC TGGCAGCTCC CGCACCTGCT TCCCAGGTGG TCTCCCCTCC 
AGTCAAAGCC AGCACAAGTA CCACTTTGAG AAGGCCTTTG TGGTGTCTCA GGCTGGAAGC 
TGCAGGGTTA TGGCATACTG TGACTCACTG AGCTGTCTCG TGGTATCACA GCCTTCTCCA 
CAGTCAACTT TTATTCCTGG TTGTGGTGTT AAGATGATGA GCGCGGCCAA CCTGAAGAGC 
AGTCAGTACA TCCCTATCCA TAGTAAACAG ATTCGGGGAC TGGCTTTTGG CAGTCGAGCA 
GATGGCTTGC TGCTGTCAGC TGCTCTGGAC AACACTCTCA AATTAACCAG CTTGGCAACA 
AACACTGTGG TACAGACATA CAATACTGGT CGTCCTGTCT GGAGTTGCTG CTGGTGTCTT 
GATGATACAA ACTACATCTA TGCTGGGCTG GTTAATGGCT CTATCATGGT ATATGATTTG 
AGAGAGACAA GCTCTCACGT CCAGGAGCTG GCCCCTCAGA AGTCCAGGTG CCCCATGGTG 
TCACTGTCCT ACCTGCCACG CATGGCCTCA GCATCATTGC CTTATGGTGG AATAGTAGCT 
GGGACCTTGG AAGGAGCCTG CTTTTGGGAA CAGAAAGCTG GCACCTCTTA CCGGCCTCAT 
CACTTGCCAC TGGAACCTGG AGGATGCATT GATATTCAAA CGGAGATCAA CACACGGCAC 
TGCCTTGCAA CATACAGGCC TAGTAAAAAT AATCCTTGCC TGCGTTGCGT GATGATGGAA 
CTGACCTGCA GCCCACTGAC AGAAGCCTCT GAAGATGTGG TGTGCTCCTC TAACCCAGTT 
CAGACTTTCA GTGCTGGGCC CACTTGCAAG TTGTTGACCA AAAATGCCAT TTTTCAGAGC 
CCAGAGGAGG ATGGCAGTAT TCTTGTGTGT GCTGGCGATG AGGCCTCTAA TTCTGCCATG 
TTATGGGATG CTGGAAGTGG CACCTTACTG CAGAAGCTGC AGGCTGACCT ACCTGTGTTG 
GATATCTGCC CCTTTGAAGT GAACCAAACC CACCTCCTGG CTACGCTCAC TGAGAAAATG 
GTCAAGATCT ACAAGTGGCA GTGA