ncstn cDNA ORF clone, Stegastes partitus(bicolor damselfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncstn
Entrez Gene ID	103367098
Full Name	nicastrin
Gene Type	protein-coding
Organism	Stegastes partitus(bicolor damselfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008295019.1	XP_008293241.1	nicastrin

Pathways from BioSystems
Gene Ontology

The following ncstn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncstn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt54574	XM_008295019.1 Latest version!	Stegastes partitus nicastrin (ncstn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncstn ( XM_008295019.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008293241.1 Stegastes partitus nicastrin (ncstn), mRNA.

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CloneID	OSt54574
Clone ID Related Accession (Same CDS sequence)	XM_008295019.1
Accession Version	XM_008295019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	nicastrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007578746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC TGTCGAACAA ATGGATTATT AATTTGATAG TTTGTTTGTT TTCCGTCGGT 
GTCAGCTGCA ACTCAGTGGA AAAGAAGATT TATGTGCATC TCAATTACAC AGTCCCTTGT 
GTGCGGCTGC TAAATGCAAC ACATCAAATA GGCTGCCAGT CCTCTTTGTC AGGTGATGTG 
GGTGTGCTTC ATGTGCTCCA GTCAGAGGAA AACCTGGACT GGGTCCTGAG TTCTGGTCCC 
AATCCTCCTT ATATGGTTAT TCTGGAGTCC TCGCTGTTTA CCAGATCCGT CGCGATAAAG 
CTGAAGAACG GCTCCAACAG GGTGGCCGGC GTCGTGGTGG TGGCGCCGAG CACAAATCCA 
GTTGAGGGCT TCTCTCCTCA CACCTCCTGC CCCAACGAGA ACTCAGGCGT TTATTCAGAG 
AGCTACGACC CGGCGTTGGC CCACTGTAAA GAGACAGTTT GGAACCCTCT GGGAAACGGT 
TTGTCCTACG AGGAGTATGA CTTCCCCATC TTCTCCCTGA AAGACGACAA TGACACCGCT 
GTCATCCGAC AGTGCTACCT TGATCATAAC CACGCTGTGA ACGGCAGCAG GCCTCAGTAT 
CCACTGTGCG CAATGCAGCT CTACTCCCAC ATGTCTGCCG TCACCGACAC GGCCACCTGC 
ATGAGGAGAA ATGACATCAA CTTCAGCATC AGCCCAGAGA TGGTGTGTGA CCCGCTGGGT 
GACTTTAACG TTTGGGCCTC CACCAAGCCT CTGAACAACA CCGAGAAGGG CCACAAGATG 
GGGGAGAGTG TGGTCATCGC AGCAGCCAGA CTCGACAGCA GGTCCTTCTT CTTCGACGTC 
GCTCCGGGAG CAGAGAGTGC ATCTTCAGGC TTCATCACCC TGCTGGCGGC TGCACACGCT 
CTGCGTAACG CCACCCAGGA GGCCCCACCC AACCGCACCA TCCTCTACAC CTTCTTCCAA 
GGGGAAACTT TCGACTACAT CGGCAGTTCG AGGATGGTGT ACGACATGGA GAATAATCAG 
TTCACTGTGG ACCTGGACAA TGTTCACTCA GTGCTGGAGA TTGGACAGGT GGGACTCCGT 
GCCAACTCCA GACTCTGGCT TCACTCTGAT CCTGTTTCTA TGAGAAACAG CAGCGTCAAT 
GAAGAGGTGA GGAAGCTCAT CAACAACCTG CAGTCGGCTG CTACAAGTGT AGGCATCCCA 
GTCGCAGAAC CCGGTGTCTC TCAGGCGCTG CCTCCTGCGT CCTTCCAGCG CTTCCTGCGA 
GCTCGGCCGA TCCCCGGTGT TGTTATCGAG AATCACCAGT TTAACTTCAC CAACAAATAC 
TATGAGAGTA TGTATGACAA CGCAGAGTAT CTGAGCATTT CTTACCCACC AAACCTGACG 
CCGGAGGAGC AGCTGGAGTT CGTCACTGAA ACTGCAAAGG CTCTGACTGA AGTGGCGACG 
ATGGTGGCTC GAGCGCTCTA CGTGCAGGCA GGAGGAGCAG AGAGTCAGAT GAACAGCATA 
AACGCAGACC CTCAGATAGT GAGCCAAATG CTGTACGGTT TCCTTGTTCG GTCAAACAAC 
ACCTGGTTCC AGCAGCTGAT ACCCTCCGAC CTCGCGAGTC ATCTGAATGA CAGACCCACA 
AACTTCTACG TCGGCGTGAG TCAGCAGTCG AGTGAGCCGA CTCTTCTGGT CCAGTACCTG 
CTGGCCAACC TGACCGGCAG CACCGTCAAC GTCACCCAGG ACAACTGCCA GAACCAGAGA 
GTGGACGAGA ACGACAAGGA GAGCAAACAT ACGTTCACCT ACATGTGGGT TCAAGGCGCA 
GCGCCTCCCA ACAGCACAGA ACGGGCGGGT TTCTGCGTTC GCTCCACGGT CCGTCTCTCC 
AGAGCAGTGT CCCCGGCCTT CGAGCTGCGA GAGTACACCT CCAAAGATTA CTCAACGTGG 
ACAGAGTCCA GGTGGAAGAG CATCAAAGGA CGCATATTCC TGGTGGCCAG TCACGAACTA 
GAGATGTTGA CGCTCGGCGT GGGTTTCGGC GTGCTGCTAA CGTCCCTCCT CCTAACATAC 
ATCATCAGCT CGAAGGCCGA CATCCTGTTC AGCTCGGGGA GGGAGCCCAC CAGCGCCACC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008293241.1
CDS	34..2139
Translation	MDLLSNKWIINLIVCLFSVGVSCNSVEKKIYVHLNYTVPCVRLLNATHQIGCQSSLSGDVGVLHVLQSEENLDWVLSSGPNPPYMVILESSLFTRSVAIKLKNGSNRVAGVVVVAPSTNPVEGFSPHTSCPNENSGVYSESYDPALAHCKETVWNPLGNGLSYEEYDFPIFSLKDDNDTAVIRQCYLDHNHAVNGSRPQYPLCAMQLYSHMSAVTDTATCMRRNDINFSISPEMVCDPLGDFNVWASTKPLNNTEKGHKMGESVVIAAARLDSRSFFFDVAPGAESASSGFITLLAAAHALRNATQEAPPNRTILYTFFQGETFDYIGSSRMVYDMENNQFTVDLDNVHSVLEIGQVGLRANSRLWLHSDPVSMRNSSVNEEVRKLINNLQSAATSVGIPVAEPGVSQALPPASFQRFLRARPIPGVVIENHQFNFTNKYYESMYDNAEYLSISYPPNLTPEEQLEFVTETAKALTEVATMVARALYVQAGGAESQMNSINADPQIVSQMLYGFLVRSNNTWFQQLIPSDLASHLNDRPTNFYVGVSQQSSEPTLLVQYLLANLTGSTVNVTQDNCQNQRVDENDKESKHTFTYMWVQGAAPPNSTERAGFCVRSTVRLSRAVSPAFELREYTSKDYSTWTESRWKSIKGRIFLVASHELEMLTLGVGFGVLLTSLLLTYIISSKADILFSSGREPTSATY

Target ORF information:

RefSeq Version	XM_008295019.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus nicastrin (ncstn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008295019.1

ORF Insert Sequence:

ATGGATTTGC TGTCGAACAA ATGGATTATT AATTTGATAG TTTGTTTGTT TTCCGTCGGT 
GTCAGCTGCA ACTCAGTGGA AAAGAAGATT TATGTGCATC TCAATTACAC AGTCCCTTGT 
GTGCGGCTGC TAAATGCAAC ACATCAAATA GGCTGCCAGT CCTCTTTGTC AGGTGATGTG 
GGTGTGCTTC ATGTGCTCCA GTCAGAGGAA AACCTGGACT GGGTCCTGAG TTCTGGTCCC 
AATCCTCCTT ATATGGTTAT TCTGGAGTCC TCGCTGTTTA CCAGATCCGT CGCGATAAAG 
CTGAAGAACG GCTCCAACAG GGTGGCCGGC GTCGTGGTGG TGGCGCCGAG CACAAATCCA 
GTTGAGGGCT TCTCTCCTCA CACCTCCTGC CCCAACGAGA ACTCAGGCGT TTATTCAGAG 
AGCTACGACC CGGCGTTGGC CCACTGTAAA GAGACAGTTT GGAACCCTCT GGGAAACGGT 
TTGTCCTACG AGGAGTATGA CTTCCCCATC TTCTCCCTGA AAGACGACAA TGACACCGCT 
GTCATCCGAC AGTGCTACCT TGATCATAAC CACGCTGTGA ACGGCAGCAG GCCTCAGTAT 
CCACTGTGCG CAATGCAGCT CTACTCCCAC ATGTCTGCCG TCACCGACAC GGCCACCTGC 
ATGAGGAGAA ATGACATCAA CTTCAGCATC AGCCCAGAGA TGGTGTGTGA CCCGCTGGGT 
GACTTTAACG TTTGGGCCTC CACCAAGCCT CTGAACAACA CCGAGAAGGG CCACAAGATG 
GGGGAGAGTG TGGTCATCGC AGCAGCCAGA CTCGACAGCA GGTCCTTCTT CTTCGACGTC 
GCTCCGGGAG CAGAGAGTGC ATCTTCAGGC TTCATCACCC TGCTGGCGGC TGCACACGCT 
CTGCGTAACG CCACCCAGGA GGCCCCACCC AACCGCACCA TCCTCTACAC CTTCTTCCAA 
GGGGAAACTT TCGACTACAT CGGCAGTTCG AGGATGGTGT ACGACATGGA GAATAATCAG 
TTCACTGTGG ACCTGGACAA TGTTCACTCA GTGCTGGAGA TTGGACAGGT GGGACTCCGT 
GCCAACTCCA GACTCTGGCT TCACTCTGAT CCTGTTTCTA TGAGAAACAG CAGCGTCAAT 
GAAGAGGTGA GGAAGCTCAT CAACAACCTG CAGTCGGCTG CTACAAGTGT AGGCATCCCA 
GTCGCAGAAC CCGGTGTCTC TCAGGCGCTG CCTCCTGCGT CCTTCCAGCG CTTCCTGCGA 
GCTCGGCCGA TCCCCGGTGT TGTTATCGAG AATCACCAGT TTAACTTCAC CAACAAATAC 
TATGAGAGTA TGTATGACAA CGCAGAGTAT CTGAGCATTT CTTACCCACC AAACCTGACG 
CCGGAGGAGC AGCTGGAGTT CGTCACTGAA ACTGCAAAGG CTCTGACTGA AGTGGCGACG 
ATGGTGGCTC GAGCGCTCTA CGTGCAGGCA GGAGGAGCAG AGAGTCAGAT GAACAGCATA 
AACGCAGACC CTCAGATAGT GAGCCAAATG CTGTACGGTT TCCTTGTTCG GTCAAACAAC 
ACCTGGTTCC AGCAGCTGAT ACCCTCCGAC CTCGCGAGTC ATCTGAATGA CAGACCCACA 
AACTTCTACG TCGGCGTGAG TCAGCAGTCG AGTGAGCCGA CTCTTCTGGT CCAGTACCTG 
CTGGCCAACC TGACCGGCAG CACCGTCAAC GTCACCCAGG ACAACTGCCA GAACCAGAGA 
GTGGACGAGA ACGACAAGGA GAGCAAACAT ACGTTCACCT ACATGTGGGT TCAAGGCGCA 
GCGCCTCCCA ACAGCACAGA ACGGGCGGGT TTCTGCGTTC GCTCCACGGT CCGTCTCTCC 
AGAGCAGTGT CCCCGGCCTT CGAGCTGCGA GAGTACACCT CCAAAGATTA CTCAACGTGG 
ACAGAGTCCA GGTGGAAGAG CATCAAAGGA CGCATATTCC TGGTGGCCAG TCACGAACTA 
GAGATGTTGA CGCTCGGCGT GGGTTTCGGC GTGCTGCTAA CGTCCCTCCT CCTAACATAC 
ATCATCAGCT CGAAGGCCGA CATCCTGTTC AGCTCGGGGA GGGAGCCCAC CAGCGCCACC 
TACTGA