apaf1 cDNA ORF clone, Stegastes partitus(bicolor damselfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	apaf1
Entrez Gene ID	103355187
Full Name	apoptotic peptidase activating factor 1
Gene Type	protein-coding
Organism	Stegastes partitus(bicolor damselfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008278886.1	XP_008277108.1	apoptotic protease-activating factor 1

Pathways from BioSystems
Gene Ontology

The following apaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the apaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt41476	XM_008278886.1 Latest version!	Stegastes partitus apoptotic peptidase activating factor 1 (apaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

apaf1 ( XM_008278886.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008277108.1 Stegastes partitus apoptotic peptidase activating factor 1 (apaf1), mRNA.

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CloneID	OSt41476
Clone ID Related Accession (Same CDS sequence)	XM_008278886.1
Accession Version	XM_008278886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	apoptotic protease-activating factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007577867.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGG AGGAAGGGGC GCGGAGCTGT CTGCTGCGCT TTCGCCACAG ACTGGAGCAG 
GACATCAAAG CCGGCTACCT GATGGACCAC ATGATCAGTG ACGGTGTGAT GACTGTGGAT 
GAGGAGGAGA AGATCAGGAT TAAGCCCACC AGAAAGGATC AGGCTGCAGC CCTGCTGGAA 
CTCTTACTGA TGAAAGACAA TCGTGCCTAC ATCTCTTTCT ACAACGCCCT GGTCAAGGAG 
GCATACGATG ATTTGGCACA TATGCTCCAC GGCGACCTAC CGTGCGTTGT ACCTGATGCA 
CATAAGAGCT CCTCTGATAG CCTTACATCT TATGTCCAGA CGGTGCTTAG TGAAGGCGGA 
GTGCCACAGA GGCCCGTGGT GTTTGTCAGC AGACCAGAGC TGGTGAACCG GGTCAGGGAG 
AAACTGTACC GGCTCCAGAA GGATACTGGC TGGGTCACGG TCTTCGGCAT GGCCGGCTCG 
GGCAAGTCTG TCCTCGCTGC CGAGGCTGTC AGACACCATG GACTCATCGA AGACTGTTTT 
CCTGGAGGTA TCCACTGGCT GGCCATTGGT CAGCTGGACA AGCCGGACCT GCTGGTGAAG 
ATGCAGTCAT TGTGTTTTCG TCTGGAGCAG GGCCTGGATT CCCAGTCCCT CTCCAGGCCC 
CCCACCTCTC TGGATGAAGC CAAGGAGCGT CTGCGCTTCC TCATGCTGCG GAGATATCCC 
AGATCTCTGC TGATTCTAGA TGACATCTGG GATAGTACAG TGCTGAAGGC GTTTGATATC 
CACTGTCGGA TACTTCTAAC CACCAGAAAT AGAAGCCTTA CTGACACTGT TAGCGGTGCT 
AAACATGAGG TGGGAGTGCA GAGTGGTCTG GATGAAAACA AGGGGCTGGA GATCCTTGCT 
CTGTACACTA AAATTAAGCC TCACGGGCTT CCTGAGGAGG CTCGCAGCAT CGTAAGAGAG 
TGTAAAGGCT CCCCTCTGGT GGTGTCTCTG ATTGGGGCTT TGCTCAAAGA CAAACCCAAC 
CGTTGGAGCT ACTACCTCCG CCAGCTGCAG CAGAAACAGT TTAAACGTAT TCGGAAGTCG 
TCATCTTACG ACTACGATGC CCTGGATCAG GCCATGGCCG CGAGCATTGA GGTCCTACCT 
GACGAGCATC GAGAGCTCTA CAAGGACTTA ACTGTGCTGG AGAAGGACAT CAAAGTCCCT 
GCTAAGGTGC TGTCTGTTCT GTGGGATCTG GAGCCGGAGG AGGTGGAGGA CATTCTCCAG 
GAGTTTGTTG ACAAGTCTCT GCTTTTTGTG GACAGTCACA ACAAACCCTA CCTGTACTAC 
CTTCACGACC TCCAGCTGGA CTTCCTCGTG GAGCAGAATC GAACCCAGCT CGAGATCCTT 
CACAGTAAGG TGGTGAATCA GTACCAACAG CACTACAGAG ATGGTCCTCC CACCTCAGGA 
GATGAGGAAT GTCTCTACTG GATCAGATTC TTGACCTACC ATATGGCCAA AGCCAACCTC 
TCACAGGAGC TGTACTCCCT CATGTTCTCT CTGGATTGGA TCAGCACCAA GGCCCAGATA 
ATGGGTCCAG CTCACCTCAT CAATGACTAC ATGGAGTATG GATCCATTCT GGACAAAGAG 
AACAGCGAAG TGCGAAGTCA GTTCCAGGAG TTTCTGTCTC AGAACGGCCA CCAGCTGGAG 
GAGCGTCCTT TCCCTGACGT TGTGCAGCTC GCTCTGTCTC AGCCACACAA CTCTGAGGTG 
CACAGACAGG CCCGGCTACA GGCTCAGGAC CGGACCAGCG CTGGGAAACT CTACTTTGAT 
CTAGTGAATA AGAGCAGCGT AGAGAATCTG TCCCGTTTGG TGATCCACCC CCACCAGGGC 
TCCATCTACT CTGCCTGCTT CTCTCATGAT GGAACCAAAA TTGCCTCTTG TGGAGCAAGC 
AAGACACTCA AGGTGTTTAA AAGTACCTCG GGCGAGAAGC TCACAGAGAT TCAGGCCCAC 
GAAGACGAAG TGCTTTGTTG TGCCTTCTCC CCTGATGACC ATCTCCTAGC AACCTGCTCT 
ACTGACAGGA AAATCAAGGT GTGGAACGCA GAGCGCGCCA TGCTACTGAG GGTTTTTGAG 
GAGGAGCACG AGGAGCAGAT CAACCACTGT CAGTTCACCA ACACAACTCA GCGCCTCCTG 
CTGGCCACAT GCTCCAACGA CGAATTCATG AACGTTAAGC TGTGGAACCT CAACAAACCG 
TCCTCCCAGA ACACCATGTT CGGGCACTTT GCGCCCGTCA GCCACTGCTG TTTCTCCCCT 
GATGACAGTT ATTTGTCCAC TTCCTCCAAC GATGGCACCG TTAAGCTATT TCAAGTGTCG 
TCTGCCAACG AGTGGAAGAC AATCGGTGTG AGGGACTTGG TTACAGAGAG CGATGAAGAG 
GTCCTCGTCA AGTGCAGCAC CTGGACCGGA GACGGGAAAC GCATCATATG TGCAGTCAGA 
AATGCTGTTC TGGTGTTTGA CGTGGAGACA TCAGACATGT TGTTGGAGAT CAGGACCAGT 
CGCCTGAGCA CGGTGCAGTA CTGTGACGCC TGTCCCACCA CGAACCTGCT GGCCATCGCG 
TTTTCAAATT ATACCGTGGA GCTGTGGGAT CTTGAGGCCA ATAAGAAGAT GGCCGACTGC 
AGCGGTCACC TCAGTTGGGT CCAGCGTGTC CAGTTTTCTC CCGACGGGTC GCAGCTGCTC 
TCCTGCTCTG ACGACCAGAC TGTGAGGCTG TGGGAGACTA ATAAGGTTCA CACATCCTCA 
GCAATCTGCC TGAAGAGAGA TTCTGACGTT CTCTTCAATG GTGAGGAAAT CATCGTGTCA 
GCTGCAGACA ACTGCAACAG ACTGCAGGTG CGTGATGGCA GAACGGGATC GGTGCTGTTC 
CAGTCAGAGG AGAAATCATC CAGGATTAGG TGTACCTGTA TGTGCAGGCA GCCCTCTGCT 
GTGGTCCTGG GCCAGGAGGA CGGCACTGTG CAGGTGTTGG AGGTGCCCTC TGGGAAGCTT 
CTGGCTACTC TGCTGGGACA CACCAAGACT GTGCTGCACT GCCAGTTCAG CCAGAACGGC 
CAGACGCTCC TCACATCCTC TGAGGACGCC ACCATAAGGG TGTGGAGGTG GCAATCAGGT 
GAGTGTAAAG TACTTCGGGG TCACAAGGAG CAAGTCAGAT GCTTCTCTGT CCTCTCCGAC 
GCACCAAACA ACTCCAGACT GCTGTCCTGG TCCTTCGACG GTACTGTGAA GGTGTGGGAC 
ACGGAGAGCG GGGAGATACT GCAGGACATC GAGGCTCATC CAGGAACTAT TCTGGCCTGT 
CATGTCTCGC CAGACGGATG CCTCTTTGCC ACCACCTCTG CTGACAAGAC TGCTAAGTTG 
TGGCACTGTG AGTCCTGGCA GTGTGTCCAC ACATTGAACG GTCACCAAGA CTGCGTCCGA 
AGCTGCCGAT TCTCATGGGA CAGCCAACGC CTCGCAACAG GAGATGACAA CGGAGAGATC 
CGGCTCTGGA GCGTCAAGGA TGGCTCTTTA CTGAAGATTT GTTCCCGGGA CACTAAGGAC 
GGCATGGACT CGCTCCACGG AGGCTGGGTG ACGGATCTGC ACTTCTCCCC TGATAACTCT 
CTTCTGGTCT CCACTGGAGG CTACATCAAG TGGTGGAACG TGGAGAAAGG AGAAGCCCTG 
CAGACCTTCT ATCCGACGGG AAGCGCTTTG AAGAAGATCC ACGTGTCATC AGACTTCTCC 
ACCTTTGTTA CCATCGACAA CATCGGCATC CTGTACATCC TCCAGAGGGT GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008277108.1
CDS	162..3938
Translation	MALEEGARSCLLRFRHRLEQDIKAGYLMDHMISDGVMTVDEEEKIRIKPTRKDQAAALLELLLMKDNRAYISFYNALVKEAYDDLAHMLHGDLPCVVPDAHKSSSDSLTSYVQTVLSEGGVPQRPVVFVSRPELVNRVREKLYRLQKDTGWVTVFGMAGSGKSVLAAEAVRHHGLIEDCFPGGIHWLAIGQLDKPDLLVKMQSLCFRLEQGLDSQSLSRPPTSLDEAKERLRFLMLRRYPRSLLILDDIWDSTVLKAFDIHCRILLTTRNRSLTDTVSGAKHEVGVQSGLDENKGLEILALYTKIKPHGLPEEARSIVRECKGSPLVVSLIGALLKDKPNRWSYYLRQLQQKQFKRIRKSSSYDYDALDQAMAASIEVLPDEHRELYKDLTVLEKDIKVPAKVLSVLWDLEPEEVEDILQEFVDKSLLFVDSHNKPYLYYLHDLQLDFLVEQNRTQLEILHSKVVNQYQQHYRDGPPTSGDEECLYWIRFLTYHMAKANLSQELYSLMFSLDWISTKAQIMGPAHLINDYMEYGSILDKENSEVRSQFQEFLSQNGHQLEERPFPDVVQLALSQPHNSEVHRQARLQAQDRTSAGKLYFDLVNKSSVENLSRLVIHPHQGSIYSACFSHDGTKIASCGASKTLKVFKSTSGEKLTEIQAHEDEVLCCAFSPDDHLLATCSTDRKIKVWNAERAMLLRVFEEEHEEQINHCQFTNTTQRLLLATCSNDEFMNVKLWNLNKPSSQNTMFGHFAPVSHCCFSPDDSYLSTSSNDGTVKLFQVSSANEWKTIGVRDLVTESDEEVLVKCSTWTGDGKRIICAVRNAVLVFDVETSDMLLEIRTSRLSTVQYCDACPTTNLLAIAFSNYTVELWDLEANKKMADCSGHLSWVQRVQFSPDGSQLLSCSDDQTVRLWETNKVHTSSAICLKRDSDVLFNGEEIIVSAADNCNRLQVRDGRTGSVLFQSEEKSSRIRCTCMCRQPSAVVLGQEDGTVQVLEVPSGKLLATLLGHTKTVLHCQFSQNGQTLLTSSEDATIRVWRWQSGECKVLRGHKEQVRCFSVLSDAPNNSRLLSWSFDGTVKVWDTESGEILQDIEAHPGTILACHVSPDGCLFATTSADKTAKLWHCESWQCVHTLNGHQDCVRSCRFSWDSQRLATGDDNGEIRLWSVKDGSLLKICSRDTKDGMDSLHGGWVTDLHFSPDNSLLVSTGGYIKWWNVEKGEALQTFYPTGSALKKIHVSSDFSTFVTIDNIGILYILQRVA

Target ORF information:

RefSeq Version	XM_008278886.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus apoptotic peptidase activating factor 1 (apaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008278886.1

ORF Insert Sequence:

ATGGCGCTGG AGGAAGGGGC GCGGAGCTGT CTGCTGCGCT TTCGCCACAG ACTGGAGCAG 
GACATCAAAG CCGGCTACCT GATGGACCAC ATGATCAGTG ACGGTGTGAT GACTGTGGAT 
GAGGAGGAGA AGATCAGGAT TAAGCCCACC AGAAAGGATC AGGCTGCAGC CCTGCTGGAA 
CTCTTACTGA TGAAAGACAA TCGTGCCTAC ATCTCTTTCT ACAACGCCCT GGTCAAGGAG 
GCATACGATG ATTTGGCACA TATGCTCCAC GGCGACCTAC CGTGCGTTGT ACCTGATGCA 
CATAAGAGCT CCTCTGATAG CCTTACATCT TATGTCCAGA CGGTGCTTAG TGAAGGCGGA 
GTGCCACAGA GGCCCGTGGT GTTTGTCAGC AGACCAGAGC TGGTGAACCG GGTCAGGGAG 
AAACTGTACC GGCTCCAGAA GGATACTGGC TGGGTCACGG TCTTCGGCAT GGCCGGCTCG 
GGCAAGTCTG TCCTCGCTGC CGAGGCTGTC AGACACCATG GACTCATCGA AGACTGTTTT 
CCTGGAGGTA TCCACTGGCT GGCCATTGGT CAGCTGGACA AGCCGGACCT GCTGGTGAAG 
ATGCAGTCAT TGTGTTTTCG TCTGGAGCAG GGCCTGGATT CCCAGTCCCT CTCCAGGCCC 
CCCACCTCTC TGGATGAAGC CAAGGAGCGT CTGCGCTTCC TCATGCTGCG GAGATATCCC 
AGATCTCTGC TGATTCTAGA TGACATCTGG GATAGTACAG TGCTGAAGGC GTTTGATATC 
CACTGTCGGA TACTTCTAAC CACCAGAAAT AGAAGCCTTA CTGACACTGT TAGCGGTGCT 
AAACATGAGG TGGGAGTGCA GAGTGGTCTG GATGAAAACA AGGGGCTGGA GATCCTTGCT 
CTGTACACTA AAATTAAGCC TCACGGGCTT CCTGAGGAGG CTCGCAGCAT CGTAAGAGAG 
TGTAAAGGCT CCCCTCTGGT GGTGTCTCTG ATTGGGGCTT TGCTCAAAGA CAAACCCAAC 
CGTTGGAGCT ACTACCTCCG CCAGCTGCAG CAGAAACAGT TTAAACGTAT TCGGAAGTCG 
TCATCTTACG ACTACGATGC CCTGGATCAG GCCATGGCCG CGAGCATTGA GGTCCTACCT 
GACGAGCATC GAGAGCTCTA CAAGGACTTA ACTGTGCTGG AGAAGGACAT CAAAGTCCCT 
GCTAAGGTGC TGTCTGTTCT GTGGGATCTG GAGCCGGAGG AGGTGGAGGA CATTCTCCAG 
GAGTTTGTTG ACAAGTCTCT GCTTTTTGTG GACAGTCACA ACAAACCCTA CCTGTACTAC 
CTTCACGACC TCCAGCTGGA CTTCCTCGTG GAGCAGAATC GAACCCAGCT CGAGATCCTT 
CACAGTAAGG TGGTGAATCA GTACCAACAG CACTACAGAG ATGGTCCTCC CACCTCAGGA 
GATGAGGAAT GTCTCTACTG GATCAGATTC TTGACCTACC ATATGGCCAA AGCCAACCTC 
TCACAGGAGC TGTACTCCCT CATGTTCTCT CTGGATTGGA TCAGCACCAA GGCCCAGATA 
ATGGGTCCAG CTCACCTCAT CAATGACTAC ATGGAGTATG GATCCATTCT GGACAAAGAG 
AACAGCGAAG TGCGAAGTCA GTTCCAGGAG TTTCTGTCTC AGAACGGCCA CCAGCTGGAG 
GAGCGTCCTT TCCCTGACGT TGTGCAGCTC GCTCTGTCTC AGCCACACAA CTCTGAGGTG 
CACAGACAGG CCCGGCTACA GGCTCAGGAC CGGACCAGCG CTGGGAAACT CTACTTTGAT 
CTAGTGAATA AGAGCAGCGT AGAGAATCTG TCCCGTTTGG TGATCCACCC CCACCAGGGC 
TCCATCTACT CTGCCTGCTT CTCTCATGAT GGAACCAAAA TTGCCTCTTG TGGAGCAAGC 
AAGACACTCA AGGTGTTTAA AAGTACCTCG GGCGAGAAGC TCACAGAGAT TCAGGCCCAC 
GAAGACGAAG TGCTTTGTTG TGCCTTCTCC CCTGATGACC ATCTCCTAGC AACCTGCTCT 
ACTGACAGGA AAATCAAGGT GTGGAACGCA GAGCGCGCCA TGCTACTGAG GGTTTTTGAG 
GAGGAGCACG AGGAGCAGAT CAACCACTGT CAGTTCACCA ACACAACTCA GCGCCTCCTG 
CTGGCCACAT GCTCCAACGA CGAATTCATG AACGTTAAGC TGTGGAACCT CAACAAACCG 
TCCTCCCAGA ACACCATGTT CGGGCACTTT GCGCCCGTCA GCCACTGCTG TTTCTCCCCT 
GATGACAGTT ATTTGTCCAC TTCCTCCAAC GATGGCACCG TTAAGCTATT TCAAGTGTCG 
TCTGCCAACG AGTGGAAGAC AATCGGTGTG AGGGACTTGG TTACAGAGAG CGATGAAGAG 
GTCCTCGTCA AGTGCAGCAC CTGGACCGGA GACGGGAAAC GCATCATATG TGCAGTCAGA 
AATGCTGTTC TGGTGTTTGA CGTGGAGACA TCAGACATGT TGTTGGAGAT CAGGACCAGT 
CGCCTGAGCA CGGTGCAGTA CTGTGACGCC TGTCCCACCA CGAACCTGCT GGCCATCGCG 
TTTTCAAATT ATACCGTGGA GCTGTGGGAT CTTGAGGCCA ATAAGAAGAT GGCCGACTGC 
AGCGGTCACC TCAGTTGGGT CCAGCGTGTC CAGTTTTCTC CCGACGGGTC GCAGCTGCTC 
TCCTGCTCTG ACGACCAGAC TGTGAGGCTG TGGGAGACTA ATAAGGTTCA CACATCCTCA 
GCAATCTGCC TGAAGAGAGA TTCTGACGTT CTCTTCAATG GTGAGGAAAT CATCGTGTCA 
GCTGCAGACA ACTGCAACAG ACTGCAGGTG CGTGATGGCA GAACGGGATC GGTGCTGTTC 
CAGTCAGAGG AGAAATCATC CAGGATTAGG TGTACCTGTA TGTGCAGGCA GCCCTCTGCT 
GTGGTCCTGG GCCAGGAGGA CGGCACTGTG CAGGTGTTGG AGGTGCCCTC TGGGAAGCTT 
CTGGCTACTC TGCTGGGACA CACCAAGACT GTGCTGCACT GCCAGTTCAG CCAGAACGGC 
CAGACGCTCC TCACATCCTC TGAGGACGCC ACCATAAGGG TGTGGAGGTG GCAATCAGGT 
GAGTGTAAAG TACTTCGGGG TCACAAGGAG CAAGTCAGAT GCTTCTCTGT CCTCTCCGAC 
GCACCAAACA ACTCCAGACT GCTGTCCTGG TCCTTCGACG GTACTGTGAA GGTGTGGGAC 
ACGGAGAGCG GGGAGATACT GCAGGACATC GAGGCTCATC CAGGAACTAT TCTGGCCTGT 
CATGTCTCGC CAGACGGATG CCTCTTTGCC ACCACCTCTG CTGACAAGAC TGCTAAGTTG 
TGGCACTGTG AGTCCTGGCA GTGTGTCCAC ACATTGAACG GTCACCAAGA CTGCGTCCGA 
AGCTGCCGAT TCTCATGGGA CAGCCAACGC CTCGCAACAG GAGATGACAA CGGAGAGATC 
CGGCTCTGGA GCGTCAAGGA TGGCTCTTTA CTGAAGATTT GTTCCCGGGA CACTAAGGAC 
GGCATGGACT CGCTCCACGG AGGCTGGGTG ACGGATCTGC ACTTCTCCCC TGATAACTCT 
CTTCTGGTCT CCACTGGAGG CTACATCAAG TGGTGGAACG TGGAGAAAGG AGAAGCCCTG 
CAGACCTTCT ATCCGACGGG AAGCGCTTTG AAGAAGATCC ACGTGTCATC AGACTTCTCC 
ACCTTTGTTA CCATCGACAA CATCGGCATC CTGTACATCC TCCAGAGGGT GGCGTGA