afap1l2 cDNA ORF clone, Stegastes partitus(bicolor damselfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	afap1l2
Entrez Gene ID	103354262
Full Name	actin filament associated protein 1 like 2
Gene Type	protein-coding
Organism	Stegastes partitus(bicolor damselfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008277577.1	XP_008275799.1	actin filament-associated protein 1-like 2 isoform X1
XM_008277578.1	XP_008275800.1	actin filament-associated protein 1-like 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following afap1l2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the afap1l2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt40400	XM_008277577.1 Latest version!	Stegastes partitus actin filament associated protein 1-like 2 (afap1l2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OSt40401	XM_008277578.1 Latest version!	Stegastes partitus actin filament associated protein 1-like 2 (afap1l2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

afap1l2 ( XM_008277577.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008275799.1 Stegastes partitus actin filament associated protein 1-like 2 (afap1l2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSt40400
Clone ID Related Accession (Same CDS sequence)	XM_008277577.1
Accession Version	XM_008277577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	actin filament-associated protein 1-like 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007577832.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGC ACAAAGTGCT GGACCAGCTC CTCATGGAGC TTCACCGCTT CCTGCTCATC 
CTGGACAAGG AGACGCTGAG CGGGAACGCC ACCATCCAGA AGGGCCTGCT GTCCGATCTC 
CTCCACTCCT ACAGAGCTTC AAACGGTGCC GATGAGGAGT ATATCTACAT GAACAAAGTC 
CTCGTGACTG GAAAAGAAAG AGATGACAGA GGCGACAGAT CGGACGCCCT GGTGAACGGC 
AAAGGAGCTA AATACGTCCC CGTCCCGCAG AAGAGCCTTC CTGACCTGCC GCCCCCGAGA 
ACAGGTGTGG TGGTCCGGGA GCCGTTCCCG TTGCCTGTAG TTCCACCTCT GGCCTGTTTA 
GACGCTTCAG AGAGCTACTA TGAGGAGGCT CAGCCCTATG AGGAAACAGT CAACGATCTG 
GACTGTTTTG ATCTCCTCTC ACGGCCCTGG ATGCGAGAGC TCAGCTCTGA CAGTGTGGTC 
TATGCCGTTA TCACCAGGGA TGACGGAGAG GCGGTCAGCA GTTCGTACGA GTCCTACGAC 
GAGGAGGAGG TGACCAGAGG GAAGTCTCCG TCAGCTCAGC ACCAGTGGCC GTCGGCGGAG 
GCGTCCATCG AGCTGATGAA GGACGCTCGC ATCTGCGCCT TCCTGTGGAG GAAGAAGTGG 
CTCGGCCAGT GGGCCAAACA GCTGTGTGTC ATCAAAGAGC ATCGCCTGCT GTGCTATAAG 
AGCTCCAAGG AGCAGACGCC TCTGCTGGAT GTGAGTCTGC TGGGCTGCAG TGTCGTCTAC 
AAAGAAAAGC AGCTCAAGAG GAAAGAGCAC AAGCTGAAGA TTATTCCAGT GGGAGGGGAG 
GCCATCGTGC TGGGCCTGCA GAGCAAAGAA CAGACTGAGC AGTGGTTGAA GGTCATTCAA 
GAGATCAGTC CCAGGCCAGC TGAAAACTGT GACCCCCAAC ACTCCGCGTC CGACTCCCCG 
AGGCTTATTT GTACCAAGGG GGAGCTGAGC GAGAGGTACT CGGTGGCTTC AGAGAGCGGC 
AGCAGCACAG ACAGCCATGC TGAAACTCCA GAAAATAAAG ATGTGAAGAA AAAATACGGA 
GCAGGTTTGA AGTTCAGCAA CCTCATGAAC ATTGGCAAGA AGAAACCCTC CTCACTAGAG 
AGCCCGGAAA AATGTGTCGA CTTGTCAGGC TACCTCAACG TGCTGGTGAA CAGTCAGTGG 
CGGACCTGCT GGTGTCTTAT AAAGAACGGT CAGCTGTGGT TTTACCAAGA CAAGGGCAAG 
AACAAAGTGA GCCAGCCAGC TGTGACCCTG GAGGGCTGCA GCGTTTCGCC CGACCCCAGC 
CCCGAGCACC TCTACTCCTT CAGGATCGCC ATGGATGGCA CACAGCTGGC CACCTTGGAG 
GCGAAGACAT CAGCAGATAT GGGTCACTGG CTGGGCCTCT TGCTGTCACA AACTGGGACC 
AAGACTGACC CAGAGGAGCT GACGTACGAC TACGTCAACT CTGAGAGGAT CTCCAGCATC 
GTGAACGCTG CAAAGACTTC CCTATACTTG ATGCAGAGGA GGTACTCGGA GCCCAACACC 
TATATAGACA CCATACCTTC CATTTCTCAC AACTCTGATG AACTGTACGA CGATGTGGCA 
TCTCTAGCTG ATCCAGAGGA TGCTGAAGAG AGCAGCCTGC CAAACTGTGA GGACAACAAT 
GTCCAGGAAC ACAATGAAAC ATGCTCGCAA GATGCCAAGG ACACAGCGGG AACAGAAGAG 
GACAATGACA ATAGGATTTA CCTGGACCTT GTGCCTGTGC GCTCCTTCCT CCATACGTCC 
TCTGGGGGTA AAGGCTCGCC AGGCAAAGAA ACTTCCAGAC TTTCTCCGGT CCCTGCAGAG 
GACCAGAAGG ACTCCTCCTG TCCAAATAAA GAGGTCATTC CAACGAACCG TTCTGAGCCA 
ACGCCTCCGT TAAATGAGAT AAATTCAGCA TCCAAACCAG CACAAGAGCC AGCCCAGACT 
CCAGCACAGC CACAAACTCA ACCGGTCAAG ACCTCAGCCC AAAGCCAGGA GACCCCTAAG 
AGGACCAGCC TGGGAGTCCC ACAGGCCACC AACTCCCCCG GGGGGCACAT CCACAAGGGC 
TTAACGGTGC AATCTTCTGC TGCTGCTGCT GCCCGGCCTC ACAGCCCACA GCCCCTGCGG 
CCCAAAGCAT TTACTATAGG GTCTCCAGTT GAGGTCAAAC TGGGTAAGAA CCGCACAGAG 
GCAGACCTGC GGCGCTACAT CGATGAGCGC GACCGCCTGG AGAAAGAGAG GGAGGAGGTG 
AGGAGCAGCC TGGCGAACCT GAAGAAGGAG AGGAGGGAAG CAAAGGAGGA GCTCGGTTCC 
TGCCAAGATC CCAAGCAGCA GGCCTCACTA GAGGCCCGTC TGAAGCAGAA GGAGGAGGCT 
TGTCGAGAGG CGGAGCGCCG CAGGGTGGAG GTGGAGCTGC GATTGGTAGA AGTGAAAGAA 
AGCCTGAAGA AAGTGGAGTC GGGGCCTTTC ACGCTGGGAA CCACACTGGA CAGCAGCCTC 
CAGGACACGT CCACGCCTAA ACCAGCACCT GTGCCCACTC CCCAGCCATC ATTACCTTCT 
TCATCATCAT TATCATCATC ATCATCATCC TCCTCTTCCC AACCCGCAAA CAGCAGCGCC 
GGTGGTGCAG ATCTGGCTTC TCCGGTCAAC TCTGCCTCCG CTCTTAAAAA CAGACCCGCC 
TCCATCATGG CCACGAAAGG CAAAGTTTTG CAGAAAGCCA AGGAATGGGA GAAGAAGTCC 
ACCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008275799.1
CDS	163..2931
Translation	MEKHKVLDQLLMELHRFLLILDKETLSGNATIQKGLLSDLLHSYRASNGADEEYIYMNKVLVTGKERDDRGDRSDALVNGKGAKYVPVPQKSLPDLPPPRTGVVVREPFPLPVVPPLACLDASESYYEEAQPYEETVNDLDCFDLLSRPWMRELSSDSVVYAVITRDDGEAVSSSYESYDEEEVTRGKSPSAQHQWPSAEASIELMKDARICAFLWRKKWLGQWAKQLCVIKEHRLLCYKSSKEQTPLLDVSLLGCSVVYKEKQLKRKEHKLKIIPVGGEAIVLGLQSKEQTEQWLKVIQEISPRPAENCDPQHSASDSPRLICTKGELSERYSVASESGSSTDSHAETPENKDVKKKYGAGLKFSNLMNIGKKKPSSLESPEKCVDLSGYLNVLVNSQWRTCWCLIKNGQLWFYQDKGKNKVSQPAVTLEGCSVSPDPSPEHLYSFRIAMDGTQLATLEAKTSADMGHWLGLLLSQTGTKTDPEELTYDYVNSERISSIVNAAKTSLYLMQRRYSEPNTYIDTIPSISHNSDELYDDVASLADPEDAEESSLPNCEDNNVQEHNETCSQDAKDTAGTEEDNDNRIYLDLVPVRSFLHTSSGGKGSPGKETSRLSPVPAEDQKDSSCPNKEVIPTNRSEPTPPLNEINSASKPAQEPAQTPAQPQTQPVKTSAQSQETPKRTSLGVPQATNSPGGHIHKGLTVQSSAAAAARPHSPQPLRPKAFTIGSPVEVKLGKNRTEADLRRYIDERDRLEKEREEVRSSLANLKKERREAKEELGSCQDPKQQASLEARLKQKEEACREAERRRVEVELRLVEVKESLKKVESGPFTLGTTLDSSLQDTSTPKPAPVPTPQPSLPSSSSLSSSSSSSSSQPANSSAGGADLASPVNSASALKNRPASIMATKGKVLQKAKEWEKKSTT

Target ORF information:

RefSeq Version	XM_008277577.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus actin filament associated protein 1-like 2 (afap1l2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008277577.1

ORF Insert Sequence:

ATGGAGAAGC ACAAAGTGCT GGACCAGCTC CTCATGGAGC TTCACCGCTT CCTGCTCATC 
CTGGACAAGG AGACGCTGAG CGGGAACGCC ACCATCCAGA AGGGCCTGCT GTCCGATCTC 
CTCCACTCCT ACAGAGCTTC AAACGGTGCC GATGAGGAGT ATATCTACAT GAACAAAGTC 
CTCGTGACTG GAAAAGAAAG AGATGACAGA GGCGACAGAT CGGACGCCCT GGTGAACGGC 
AAAGGAGCTA AATACGTCCC CGTCCCGCAG AAGAGCCTTC CTGACCTGCC GCCCCCGAGA 
ACAGGTGTGG TGGTCCGGGA GCCGTTCCCG TTGCCTGTAG TTCCACCTCT GGCCTGTTTA 
GACGCTTCAG AGAGCTACTA TGAGGAGGCT CAGCCCTATG AGGAAACAGT CAACGATCTG 
GACTGTTTTG ATCTCCTCTC ACGGCCCTGG ATGCGAGAGC TCAGCTCTGA CAGTGTGGTC 
TATGCCGTTA TCACCAGGGA TGACGGAGAG GCGGTCAGCA GTTCGTACGA GTCCTACGAC 
GAGGAGGAGG TGACCAGAGG GAAGTCTCCG TCAGCTCAGC ACCAGTGGCC GTCGGCGGAG 
GCGTCCATCG AGCTGATGAA GGACGCTCGC ATCTGCGCCT TCCTGTGGAG GAAGAAGTGG 
CTCGGCCAGT GGGCCAAACA GCTGTGTGTC ATCAAAGAGC ATCGCCTGCT GTGCTATAAG 
AGCTCCAAGG AGCAGACGCC TCTGCTGGAT GTGAGTCTGC TGGGCTGCAG TGTCGTCTAC 
AAAGAAAAGC AGCTCAAGAG GAAAGAGCAC AAGCTGAAGA TTATTCCAGT GGGAGGGGAG 
GCCATCGTGC TGGGCCTGCA GAGCAAAGAA CAGACTGAGC AGTGGTTGAA GGTCATTCAA 
GAGATCAGTC CCAGGCCAGC TGAAAACTGT GACCCCCAAC ACTCCGCGTC CGACTCCCCG 
AGGCTTATTT GTACCAAGGG GGAGCTGAGC GAGAGGTACT CGGTGGCTTC AGAGAGCGGC 
AGCAGCACAG ACAGCCATGC TGAAACTCCA GAAAATAAAG ATGTGAAGAA AAAATACGGA 
GCAGGTTTGA AGTTCAGCAA CCTCATGAAC ATTGGCAAGA AGAAACCCTC CTCACTAGAG 
AGCCCGGAAA AATGTGTCGA CTTGTCAGGC TACCTCAACG TGCTGGTGAA CAGTCAGTGG 
CGGACCTGCT GGTGTCTTAT AAAGAACGGT CAGCTGTGGT TTTACCAAGA CAAGGGCAAG 
AACAAAGTGA GCCAGCCAGC TGTGACCCTG GAGGGCTGCA GCGTTTCGCC CGACCCCAGC 
CCCGAGCACC TCTACTCCTT CAGGATCGCC ATGGATGGCA CACAGCTGGC CACCTTGGAG 
GCGAAGACAT CAGCAGATAT GGGTCACTGG CTGGGCCTCT TGCTGTCACA AACTGGGACC 
AAGACTGACC CAGAGGAGCT GACGTACGAC TACGTCAACT CTGAGAGGAT CTCCAGCATC 
GTGAACGCTG CAAAGACTTC CCTATACTTG ATGCAGAGGA GGTACTCGGA GCCCAACACC 
TATATAGACA CCATACCTTC CATTTCTCAC AACTCTGATG AACTGTACGA CGATGTGGCA 
TCTCTAGCTG ATCCAGAGGA TGCTGAAGAG AGCAGCCTGC CAAACTGTGA GGACAACAAT 
GTCCAGGAAC ACAATGAAAC ATGCTCGCAA GATGCCAAGG ACACAGCGGG AACAGAAGAG 
GACAATGACA ATAGGATTTA CCTGGACCTT GTGCCTGTGC GCTCCTTCCT CCATACGTCC 
TCTGGGGGTA AAGGCTCGCC AGGCAAAGAA ACTTCCAGAC TTTCTCCGGT CCCTGCAGAG 
GACCAGAAGG ACTCCTCCTG TCCAAATAAA GAGGTCATTC CAACGAACCG TTCTGAGCCA 
ACGCCTCCGT TAAATGAGAT AAATTCAGCA TCCAAACCAG CACAAGAGCC AGCCCAGACT 
CCAGCACAGC CACAAACTCA ACCGGTCAAG ACCTCAGCCC AAAGCCAGGA GACCCCTAAG 
AGGACCAGCC TGGGAGTCCC ACAGGCCACC AACTCCCCCG GGGGGCACAT CCACAAGGGC 
TTAACGGTGC AATCTTCTGC TGCTGCTGCT GCCCGGCCTC ACAGCCCACA GCCCCTGCGG 
CCCAAAGCAT TTACTATAGG GTCTCCAGTT GAGGTCAAAC TGGGTAAGAA CCGCACAGAG 
GCAGACCTGC GGCGCTACAT CGATGAGCGC GACCGCCTGG AGAAAGAGAG GGAGGAGGTG 
AGGAGCAGCC TGGCGAACCT GAAGAAGGAG AGGAGGGAAG CAAAGGAGGA GCTCGGTTCC 
TGCCAAGATC CCAAGCAGCA GGCCTCACTA GAGGCCCGTC TGAAGCAGAA GGAGGAGGCT 
TGTCGAGAGG CGGAGCGCCG CAGGGTGGAG GTGGAGCTGC GATTGGTAGA AGTGAAAGAA 
AGCCTGAAGA AAGTGGAGTC GGGGCCTTTC ACGCTGGGAA CCACACTGGA CAGCAGCCTC 
CAGGACACGT CCACGCCTAA ACCAGCACCT GTGCCCACTC CCCAGCCATC ATTACCTTCT 
TCATCATCAT TATCATCATC ATCATCATCC TCCTCTTCCC AACCCGCAAA CAGCAGCGCC 
GGTGGTGCAG ATCTGGCTTC TCCGGTCAAC TCTGCCTCCG CTCTTAAAAA CAGACCCGCC 
TCCATCATGG CCACGAAAGG CAAAGTTTTG CAGAAAGCCA AGGAATGGGA GAAGAAGTCC 
ACCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1l2 ( XM_008277578.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008275800.1 Stegastes partitus actin filament associated protein 1-like 2 (afap1l2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSt40401
Clone ID Related Accession (Same CDS sequence)	XM_008277578.1
Accession Version	XM_008277578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	actin filament-associated protein 1-like 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007577832.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGC ACAAAGTGCT GGACCAGCTC CTCATGGAGC TTCACCGCTT CCTGCTCATC 
CTGGACAAGG AGACGCTGAG CGGGAACGCC ACCATCCAGA AGGGCCTGCT GTCCGATCTC 
CTCCACTCCT ACAGAGCTTC AAACGGTGCC GATGAGGAGT ATATCTACAT GAACAAAGTC 
CTCGTGACTG GAAAAGAAAG AGATGACAGA GGCGACAGAT CGGACGCCCT GGTGAACGGC 
AAAGGAGCTA AATACGTCCC CGTCCCGCAG AAGAGCCTTC CTGACCTGCC GCCCCCGAGA 
ACAGGTGTGG TGGTCCGGGA GCCGTTCCCG TTGCCTGTAG TTCCACCTCT GGCCTGTTTA 
GACGCTTCAG AGAGCTACTA TGAGGAGGCT CAGCCCTATG AGGAAACAGT CAACGATGAC 
GGAGAGGCGG TCAGCAGTTC GTACGAGTCC TACGACGAGG AGGAGGTGAC CAGAGGGAAG 
TCTCCGTCAG CTCAGCACCA GTGGCCGTCG GCGGAGGCGT CCATCGAGCT GATGAAGGAC 
GCTCGCATCT GCGCCTTCCT GTGGAGGAAG AAGTGGCTCG GCCAGTGGGC CAAACAGCTG 
TGTGTCATCA AAGAGCATCG CCTGCTGTGC TATAAGAGCT CCAAGGAGCA GACGCCTCTG 
CTGGATGTGA GTCTGCTGGG CTGCAGTGTC GTCTACAAAG AAAAGCAGCT CAAGAGGAAA 
GAGCACAAGC TGAAGATTAT TCCAGTGGGA GGGGAGGCCA TCGTGCTGGG CCTGCAGAGC 
AAAGAACAGA CTGAGCAGTG GTTGAAGGTC ATTCAAGAGA TCAGTCCCAG GCCAGCTGAA 
AACTGTGACC CCCAACACTC CGCGTCCGAC TCCCCGAGGC TTATTTGTAC CAAGGGGGAG 
CTGAGCGAGA GGTACTCGGT GGCTTCAGAG AGCGGCAGCA GCACAGACAG CCATGCTGAA 
ACTCCAGAAA ATAAAGATGT GAAGAAAAAA TACGGAGCAG GTTTGAAGTT CAGCAACCTC 
ATGAACATTG GCAAGAAGAA ACCCTCCTCA CTAGAGAGCC CGGAAAAATG TGTCGACTTG 
TCAGGCTACC TCAACGTGCT GGTGAACAGT CAGTGGCGGA CCTGCTGGTG TCTTATAAAG 
AACGGTCAGC TGTGGTTTTA CCAAGACAAG GGCAAGAACA AAGTGAGCCA GCCAGCTGTG 
ACCCTGGAGG GCTGCAGCGT TTCGCCCGAC CCCAGCCCCG AGCACCTCTA CTCCTTCAGG 
ATCGCCATGG ATGGCACACA GCTGGCCACC TTGGAGGCGA AGACATCAGC AGATATGGGT 
CACTGGCTGG GCCTCTTGCT GTCACAAACT GGGACCAAGA CTGACCCAGA GGAGCTGACG 
TACGACTACG TCAACTCTGA GAGGATCTCC AGCATCGTGA ACGCTGCAAA GACTTCCCTA 
TACTTGATGC AGAGGAGGTA CTCGGAGCCC AACACCTATA TAGACACCAT ACCTTCCATT 
TCTCACAACT CTGATGAACT GTACGACGAT GTGGCATCTC TAGCTGATCC AGAGGATGCT 
GAAGAGAGCA GCCTGCCAAA CTGTGAGGAC AACAATGTCC AGGAACACAA TGAAACATGC 
TCGCAAGATG CCAAGGACAC AGCGGGAACA GAAGAGGACA ATGACAATAG GATTTACCTG 
GACCTTGTGC CTGTGCGCTC CTTCCTCCAT ACGTCCTCTG GGGGTAAAGG CTCGCCAGGC 
AAAGAAACTT CCAGACTTTC TCCGGTCCCT GCAGAGGACC AGAAGGACTC CTCCTGTCCA 
AATAAAGAGG TCATTCCAAC GAACCGTTCT GAGCCAACGC CTCCGTTAAA TGAGATAAAT 
TCAGCATCCA AACCAGCACA AGAGCCAGCC CAGACTCCAG CACAGCCACA AACTCAACCG 
GTCAAGACCT CAGCCCAAAG CCAGGAGACC CCTAAGAGGA CCAGCCTGGG AGTCCCACAG 
GCCACCAACT CCCCCGGGGG GCACATCCAC AAGGGCTTAA CGGTGCAATC TTCTGCTGCT 
GCTGCTGCCC GGCCTCACAG CCCACAGCCC CTGCGGCCCA AAGCATTTAC TATAGGGTCT 
CCAGTTGAGG TCAAACTGGG TAAGAACCGC ACAGAGGCAG ACCTGCGGCG CTACATCGAT 
GAGCGCGACC GCCTGGAGAA AGAGAGGGAG GAGGTGAGGA GCAGCCTGGC GAACCTGAAG 
AAGGAGAGGA GGGAAGCAAA GGAGGAGCTC GGTTCCTGCC AAGATCCCAA GCAGCAGGCC 
TCACTAGAGG CCCGTCTGAA GCAGAAGGAG GAGGCTTGTC GAGAGGCGGA GCGCCGCAGG 
GTGGAGGTGG AGCTGCGATT GGTAGAAGTG AAAGAAAGCC TGAAGAAAGT GGAGTCGGGG 
CCTTTCACGC TGGGAACCAC ACTGGACAGC AGCCTCCAGG ACACGTCCAC GCCTAAACCA 
GCACCTGTGC CCACTCCCCA GCCATCATTA CCTTCTTCAT CATCATTATC ATCATCATCA 
TCATCCTCCT CTTCCCAACC CGCAAACAGC AGCGCCGGTG GTGCAGATCT GGCTTCTCCG 
GTCAACTCTG CCTCCGCTCT TAAAAACAGA CCCGCCTCCA TCATGGCCAC GAAAGGCAAA 
GTTTTGCAGA AAGCCAAGGA ATGGGAGAAG AAGTCCACCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008275800.1
CDS	162..2846
Translation	MEKHKVLDQLLMELHRFLLILDKETLSGNATIQKGLLSDLLHSYRASNGADEEYIYMNKVLVTGKERDDRGDRSDALVNGKGAKYVPVPQKSLPDLPPPRTGVVVREPFPLPVVPPLACLDASESYYEEAQPYEETVNDDGEAVSSSYESYDEEEVTRGKSPSAQHQWPSAEASIELMKDARICAFLWRKKWLGQWAKQLCVIKEHRLLCYKSSKEQTPLLDVSLLGCSVVYKEKQLKRKEHKLKIIPVGGEAIVLGLQSKEQTEQWLKVIQEISPRPAENCDPQHSASDSPRLICTKGELSERYSVASESGSSTDSHAETPENKDVKKKYGAGLKFSNLMNIGKKKPSSLESPEKCVDLSGYLNVLVNSQWRTCWCLIKNGQLWFYQDKGKNKVSQPAVTLEGCSVSPDPSPEHLYSFRIAMDGTQLATLEAKTSADMGHWLGLLLSQTGTKTDPEELTYDYVNSERISSIVNAAKTSLYLMQRRYSEPNTYIDTIPSISHNSDELYDDVASLADPEDAEESSLPNCEDNNVQEHNETCSQDAKDTAGTEEDNDNRIYLDLVPVRSFLHTSSGGKGSPGKETSRLSPVPAEDQKDSSCPNKEVIPTNRSEPTPPLNEINSASKPAQEPAQTPAQPQTQPVKTSAQSQETPKRTSLGVPQATNSPGGHIHKGLTVQSSAAAAARPHSPQPLRPKAFTIGSPVEVKLGKNRTEADLRRYIDERDRLEKEREEVRSSLANLKKERREAKEELGSCQDPKQQASLEARLKQKEEACREAERRRVEVELRLVEVKESLKKVESGPFTLGTTLDSSLQDTSTPKPAPVPTPQPSLPSSSSLSSSSSSSSSQPANSSAGGADLASPVNSASALKNRPASIMATKGKVLQKAKEWEKKSTT

Target ORF information:

RefSeq Version	XM_008277578.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus actin filament associated protein 1-like 2 (afap1l2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008277578.1

ORF Insert Sequence:

ATGGAGAAGC ACAAAGTGCT GGACCAGCTC CTCATGGAGC TTCACCGCTT CCTGCTCATC 
CTGGACAAGG AGACGCTGAG CGGGAACGCC ACCATCCAGA AGGGCCTGCT GTCCGATCTC 
CTCCACTCCT ACAGAGCTTC AAACGGTGCC GATGAGGAGT ATATCTACAT GAACAAAGTC 
CTCGTGACTG GAAAAGAAAG AGATGACAGA GGCGACAGAT CGGACGCCCT GGTGAACGGC 
AAAGGAGCTA AATACGTCCC CGTCCCGCAG AAGAGCCTTC CTGACCTGCC GCCCCCGAGA 
ACAGGTGTGG TGGTCCGGGA GCCGTTCCCG TTGCCTGTAG TTCCACCTCT GGCCTGTTTA 
GACGCTTCAG AGAGCTACTA TGAGGAGGCT CAGCCCTATG AGGAAACAGT CAACGATGAC 
GGAGAGGCGG TCAGCAGTTC GTACGAGTCC TACGACGAGG AGGAGGTGAC CAGAGGGAAG 
TCTCCGTCAG CTCAGCACCA GTGGCCGTCG GCGGAGGCGT CCATCGAGCT GATGAAGGAC 
GCTCGCATCT GCGCCTTCCT GTGGAGGAAG AAGTGGCTCG GCCAGTGGGC CAAACAGCTG 
TGTGTCATCA AAGAGCATCG CCTGCTGTGC TATAAGAGCT CCAAGGAGCA GACGCCTCTG 
CTGGATGTGA GTCTGCTGGG CTGCAGTGTC GTCTACAAAG AAAAGCAGCT CAAGAGGAAA 
GAGCACAAGC TGAAGATTAT TCCAGTGGGA GGGGAGGCCA TCGTGCTGGG CCTGCAGAGC 
AAAGAACAGA CTGAGCAGTG GTTGAAGGTC ATTCAAGAGA TCAGTCCCAG GCCAGCTGAA 
AACTGTGACC CCCAACACTC CGCGTCCGAC TCCCCGAGGC TTATTTGTAC CAAGGGGGAG 
CTGAGCGAGA GGTACTCGGT GGCTTCAGAG AGCGGCAGCA GCACAGACAG CCATGCTGAA 
ACTCCAGAAA ATAAAGATGT GAAGAAAAAA TACGGAGCAG GTTTGAAGTT CAGCAACCTC 
ATGAACATTG GCAAGAAGAA ACCCTCCTCA CTAGAGAGCC CGGAAAAATG TGTCGACTTG 
TCAGGCTACC TCAACGTGCT GGTGAACAGT CAGTGGCGGA CCTGCTGGTG TCTTATAAAG 
AACGGTCAGC TGTGGTTTTA CCAAGACAAG GGCAAGAACA AAGTGAGCCA GCCAGCTGTG 
ACCCTGGAGG GCTGCAGCGT TTCGCCCGAC CCCAGCCCCG AGCACCTCTA CTCCTTCAGG 
ATCGCCATGG ATGGCACACA GCTGGCCACC TTGGAGGCGA AGACATCAGC AGATATGGGT 
CACTGGCTGG GCCTCTTGCT GTCACAAACT GGGACCAAGA CTGACCCAGA GGAGCTGACG 
TACGACTACG TCAACTCTGA GAGGATCTCC AGCATCGTGA ACGCTGCAAA GACTTCCCTA 
TACTTGATGC AGAGGAGGTA CTCGGAGCCC AACACCTATA TAGACACCAT ACCTTCCATT 
TCTCACAACT CTGATGAACT GTACGACGAT GTGGCATCTC TAGCTGATCC AGAGGATGCT 
GAAGAGAGCA GCCTGCCAAA CTGTGAGGAC AACAATGTCC AGGAACACAA TGAAACATGC 
TCGCAAGATG CCAAGGACAC AGCGGGAACA GAAGAGGACA ATGACAATAG GATTTACCTG 
GACCTTGTGC CTGTGCGCTC CTTCCTCCAT ACGTCCTCTG GGGGTAAAGG CTCGCCAGGC 
AAAGAAACTT CCAGACTTTC TCCGGTCCCT GCAGAGGACC AGAAGGACTC CTCCTGTCCA 
AATAAAGAGG TCATTCCAAC GAACCGTTCT GAGCCAACGC CTCCGTTAAA TGAGATAAAT 
TCAGCATCCA AACCAGCACA AGAGCCAGCC CAGACTCCAG CACAGCCACA AACTCAACCG 
GTCAAGACCT CAGCCCAAAG CCAGGAGACC CCTAAGAGGA CCAGCCTGGG AGTCCCACAG 
GCCACCAACT CCCCCGGGGG GCACATCCAC AAGGGCTTAA CGGTGCAATC TTCTGCTGCT 
GCTGCTGCCC GGCCTCACAG CCCACAGCCC CTGCGGCCCA AAGCATTTAC TATAGGGTCT 
CCAGTTGAGG TCAAACTGGG TAAGAACCGC ACAGAGGCAG ACCTGCGGCG CTACATCGAT 
GAGCGCGACC GCCTGGAGAA AGAGAGGGAG GAGGTGAGGA GCAGCCTGGC GAACCTGAAG 
AAGGAGAGGA GGGAAGCAAA GGAGGAGCTC GGTTCCTGCC AAGATCCCAA GCAGCAGGCC 
TCACTAGAGG CCCGTCTGAA GCAGAAGGAG GAGGCTTGTC GAGAGGCGGA GCGCCGCAGG 
GTGGAGGTGG AGCTGCGATT GGTAGAAGTG AAAGAAAGCC TGAAGAAAGT GGAGTCGGGG 
CCTTTCACGC TGGGAACCAC ACTGGACAGC AGCCTCCAGG ACACGTCCAC GCCTAAACCA 
GCACCTGTGC CCACTCCCCA GCCATCATTA CCTTCTTCAT CATCATTATC ATCATCATCA 
TCATCCTCCT CTTCCCAACC CGCAAACAGC AGCGCCGGTG GTGCAGATCT GGCTTCTCCG 
GTCAACTCTG CCTCCGCTCT TAAAAACAGA CCCGCCTCCA TCATGGCCAC GAAAGGCAAA 
GTTTTGCAGA AAGCCAAGGA ATGGGAGAAG AAGTCCACCA CCTAG