gfm1 cDNA ORF clone, Sinocyclocheilus anshuiensis

Gene
Clones
gRNAs

Summary

Gene Symbol	gfm1
Entrez Gene ID	107701305
Full Name	G elongation factor mitochondrial 1
Gene Type	protein-coding
Organism	Sinocyclocheilus anshuiensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_016503295.1	XP_016358781.1	elongation factor G, mitochondrial

Pathways from BioSystems
Gene Ontology

The following gfm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gfm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi129148	XM_016503295.1 Latest version!	Sinocyclocheilus anshuiensis G elongation factor, mitochondrial 1 (gfm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gfm1 ( XM_016503295.1 ) cDNA ORF clone, Sinocyclocheilus anshuiensis -> XP_016358781.1 Sinocyclocheilus anshuiensis G elongation factor, mitochondrial 1 (gfm1), mRNA.

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CloneID	OSi129148
Clone ID Related Accession (Same CDS sequence)	XM_016503295.1
Accession Version	XM_016503295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus anshuiensis
Product	elongation factor G, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015546153.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus anshuiensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTTC TGAGAGCAGC ATCAGCTTTA ACTCAACGGC ATCTGCACAA GCCGGGACGC 
GTCACACTGA AACAGGTGCT GATCAGCTGT CGCTCCTGCT CGAATGGCTT CACACCGAAC 
GAGCGGATCC GGAACATCGG CATCTCGGCG CACATCGACT CCGGGAAGAC CACACTGACC 
GAGAGAGTGC TCTACTACAC CGGCAGGATA GCGGAGATGC ACGAGGTGAG AGGGAAGGAT 
GGAGTGGGCG CGATCATGGA CTCGATGGAG TTAGAGAGAC AGAGAGGAAT CACTATCCAG 
TCCGCTGCCA CATACACCAT GTGGAAAGAT CACAACATCA ACATCATCGA CACACCAGGT 
CACGTGGATT TCACCATTGA GGTGGAGCGG GCTCTGCGTG TTCTGGATGG AGCGGTGCTG 
GTGCTGTGTG CCGTCGGAGG AGTGCAGTGT CAGACCATGA CCGTCAACCG GCAGATGAAG 
CGCTACGGCG TGCCCTTCCT CTCCTTCATC AACAAACTCG ACCGACAGGG CGCCAATCCC 
AGCCGAGCGC TGCAGCAGCT GAGGACTAAA CTGAATCATA ATGCAGCGTT TATCAACATT 
CCCATCGGAC TGGAAGGAAA TCTGCGAGGG ATCGTTGATC TGATCGAGGA GCGCAGCGTC 
TTCTTCGAAG GCCCTTTCGG TCAGAGCATT CGTTTCGATG AGATCCCGGC GGAGATGCGC 
GCCGAGGCGG CAGACCGCAG ACAGGAGCTG GTGGAGTGTG TGGCGAACGC CGATGAGACG 
CTGGGAGAGA TGTTTCTGGA GGAGAACGTT CCCACGGTGG ACGACATCAA GGCTGCTGTG 
CGCAGGGCGA CTATCCAGCG CGTGTTCACT CCTGTTCTGG TGGGCAGCGC CCTGAAGAAT 
AAAGGAGTTC AGCCGCTGCT GGACGCTGCA CTGGAGTATC TGCCGAACCC CACCGAGGTC 
AAGAACTACG CCATCCTGAA CGAGGGGGCA TCTAGTGACG GCTCCAGGAT TCTGATGGAC 
CCCACGAGAG ACGACACGCA TCCCTTCGTC GGCCTGGCCT TCAAGCTGGA GGCGGGGCGT 
TTCGGGCAGC TGACGTACGT GCGTGTGTAT CAGGGCTGTT TGAGGAAGAC GGAGTACATC 
CACAACACCC GCTCGGGGAA GAGGGTGAGG GTCCAGAGAC TGGTGCGACT CCACGCCGAT 
CAGATGGAGG ACGTGGAGGT GGTGTTTGCC GGCGATATCT GCGCTCTGTT CGGCATCGAC 
TGTGCCAGCG GAGACACCTT CACTGCCCAC ACTAATGCCA ACCTCTCCAT GGAATCCATT 
CACATTCCTG ATCCTGTGAT CTCTATGGCC ATAAGAGCCT CCAACAAGAA CGACACAGAC 
AAGTTCTCCA AGGGAATCAA TCGCTTCACC AGAGAAGATC CCACGTTTAG AGTCCACTTT 
GACACGGAGA GTAAAGAGAC CATCATCTCG GGCATGGGAG AGCTGCATCT GGAGATCTAC 
TCGCAGAGGA TGGAGAGGGA ATATAACTGT CCGTGTGTGA TGGGCAAACC CAAAGTGGCC 
TTCAGAGAGA CGGTGACCAG CGCTGTGCCG TTTGACTTCA CTCATAAGAA GCAGTCTGGA 
GGCTCGGGTC AGTACGGTAA AGTCATTGGT GTCCTGGAGC CGCTGGACCC CGAGAATTAC 
ACAAAGCTGG AGTTCAGCGA CGAGACCGTC GGGACCAACA TCCCCAAACA GTTCGTGCCG 
GCTGTGGAGA AGGGTTTCAG GGAAGCGTGT GAGAAGGGTC CTCTGACGGG ACACAAGATA 
TCTGGAGTGC GTTTCGTCCT GGAGGACGGA GCTCATCACA TGGTGGACTC TAACGAGATC 
TCCTTCATCC GGGCTGGAGA GGGCGCTGTA AAACAGGCCA TTGAGAAGTC CAGCATGGTG 
ATTCTGGAGC CGGTGATGTC TGTGGAGATC GTGGCCCCTA ATGAGTTCCA GGGGGCCGTT 
ATTGCAGGGG TCAACCGTCG TCACGGGGTC ATCACGGGAC AGGACGGGGC AGAGGGCTAT 
TTCACTCTCT ACGCTGATAT CCCGTTGAAC GACATGTTCG GATACTCGAC CGAGCTGCGC 
TCCTGCACGG AGGGTAAAGG CGAGTACACC ATGGACTACA GCAGATATCA GCCCTGTCTG 
CCCTCTGTAC AAGAAGAGCT CGTCAACAAA CACTTGGAGG CCACGGGACA GCTGCCCGCC 
AAGAAGAGCA AGTGGAAAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016358781.1
CDS	55..2298
Translation	MRLLRAASALTQRHLHKPGRVTLKQVLISCRSCSNGFTPNERIRNIGISAHIDSGKTTLTERVLYYTGRIAEMHEVRGKDGVGAIMDSMELERQRGITIQSAATYTMWKDHNINIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVNRQMKRYGVPFLSFINKLDRQGANPSRALQQLRTKLNHNAAFINIPIGLEGNLRGIVDLIEERSVFFEGPFGQSIRFDEIPAEMRAEAADRRQELVECVANADETLGEMFLEENVPTVDDIKAAVRRATIQRVFTPVLVGSALKNKGVQPLLDAALEYLPNPTEVKNYAILNEGASSDGSRILMDPTRDDTHPFVGLAFKLEAGRFGQLTYVRVYQGCLRKTEYIHNTRSGKRVRVQRLVRLHADQMEDVEVVFAGDICALFGIDCASGDTFTAHTNANLSMESIHIPDPVISMAIRASNKNDTDKFSKGINRFTREDPTFRVHFDTESKETIISGMGELHLEIYSQRMEREYNCPCVMGKPKVAFRETVTSAVPFDFTHKKQSGGSGQYGKVIGVLEPLDPENYTKLEFSDETVGTNIPKQFVPAVEKGFREACEKGPLTGHKISGVRFVLEDGAHHMVDSNEISFIRAGEGAVKQAIEKSSMVILEPVMSVEIVAPNEFQGAVIAGVNRRHGVITGQDGAEGYFTLYADIPLNDMFGYSTELRSCTEGKGEYTMDYSRYQPCLPSVQEELVNKHLEATGQLPAKKSKWKN

Target ORF information:

RefSeq Version	XM_016503295.1
Organism	Sinocyclocheilus anshuiensis
Definition	Sinocyclocheilus anshuiensis G elongation factor, mitochondrial 1 (gfm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016503295.1

ORF Insert Sequence:

ATGCGGCTTC TGAGAGCAGC ATCAGCTTTA ACTCAACGGC ATCTGCACAA GCCGGGACGC 
GTCACACTGA AACAGGTGCT GATCAGCTGT CGCTCCTGCT CGAATGGCTT CACACCGAAC 
GAGCGGATCC GGAACATCGG CATCTCGGCG CACATCGACT CCGGGAAGAC CACACTGACC 
GAGAGAGTGC TCTACTACAC CGGCAGGATA GCGGAGATGC ACGAGGTGAG AGGGAAGGAT 
GGAGTGGGCG CGATCATGGA CTCGATGGAG TTAGAGAGAC AGAGAGGAAT CACTATCCAG 
TCCGCTGCCA CATACACCAT GTGGAAAGAT CACAACATCA ACATCATCGA CACACCAGGT 
CACGTGGATT TCACCATTGA GGTGGAGCGG GCTCTGCGTG TTCTGGATGG AGCGGTGCTG 
GTGCTGTGTG CCGTCGGAGG AGTGCAGTGT CAGACCATGA CCGTCAACCG GCAGATGAAG 
CGCTACGGCG TGCCCTTCCT CTCCTTCATC AACAAACTCG ACCGACAGGG CGCCAATCCC 
AGCCGAGCGC TGCAGCAGCT GAGGACTAAA CTGAATCATA ATGCAGCGTT TATCAACATT 
CCCATCGGAC TGGAAGGAAA TCTGCGAGGG ATCGTTGATC TGATCGAGGA GCGCAGCGTC 
TTCTTCGAAG GCCCTTTCGG TCAGAGCATT CGTTTCGATG AGATCCCGGC GGAGATGCGC 
GCCGAGGCGG CAGACCGCAG ACAGGAGCTG GTGGAGTGTG TGGCGAACGC CGATGAGACG 
CTGGGAGAGA TGTTTCTGGA GGAGAACGTT CCCACGGTGG ACGACATCAA GGCTGCTGTG 
CGCAGGGCGA CTATCCAGCG CGTGTTCACT CCTGTTCTGG TGGGCAGCGC CCTGAAGAAT 
AAAGGAGTTC AGCCGCTGCT GGACGCTGCA CTGGAGTATC TGCCGAACCC CACCGAGGTC 
AAGAACTACG CCATCCTGAA CGAGGGGGCA TCTAGTGACG GCTCCAGGAT TCTGATGGAC 
CCCACGAGAG ACGACACGCA TCCCTTCGTC GGCCTGGCCT TCAAGCTGGA GGCGGGGCGT 
TTCGGGCAGC TGACGTACGT GCGTGTGTAT CAGGGCTGTT TGAGGAAGAC GGAGTACATC 
CACAACACCC GCTCGGGGAA GAGGGTGAGG GTCCAGAGAC TGGTGCGACT CCACGCCGAT 
CAGATGGAGG ACGTGGAGGT GGTGTTTGCC GGCGATATCT GCGCTCTGTT CGGCATCGAC 
TGTGCCAGCG GAGACACCTT CACTGCCCAC ACTAATGCCA ACCTCTCCAT GGAATCCATT 
CACATTCCTG ATCCTGTGAT CTCTATGGCC ATAAGAGCCT CCAACAAGAA CGACACAGAC 
AAGTTCTCCA AGGGAATCAA TCGCTTCACC AGAGAAGATC CCACGTTTAG AGTCCACTTT 
GACACGGAGA GTAAAGAGAC CATCATCTCG GGCATGGGAG AGCTGCATCT GGAGATCTAC 
TCGCAGAGGA TGGAGAGGGA ATATAACTGT CCGTGTGTGA TGGGCAAACC CAAAGTGGCC 
TTCAGAGAGA CGGTGACCAG CGCTGTGCCG TTTGACTTCA CTCATAAGAA GCAGTCTGGA 
GGCTCGGGTC AGTACGGTAA AGTCATTGGT GTCCTGGAGC CGCTGGACCC CGAGAATTAC 
ACAAAGCTGG AGTTCAGCGA CGAGACCGTC GGGACCAACA TCCCCAAACA GTTCGTGCCG 
GCTGTGGAGA AGGGTTTCAG GGAAGCGTGT GAGAAGGGTC CTCTGACGGG ACACAAGATA 
TCTGGAGTGC GTTTCGTCCT GGAGGACGGA GCTCATCACA TGGTGGACTC TAACGAGATC 
TCCTTCATCC GGGCTGGAGA GGGCGCTGTA AAACAGGCCA TTGAGAAGTC CAGCATGGTG 
ATTCTGGAGC CGGTGATGTC TGTGGAGATC GTGGCCCCTA ATGAGTTCCA GGGGGCCGTT 
ATTGCAGGGG TCAACCGTCG TCACGGGGTC ATCACGGGAC AGGACGGGGC AGAGGGCTAT 
TTCACTCTCT ACGCTGATAT CCCGTTGAAC GACATGTTCG GATACTCGAC CGAGCTGCGC 
TCCTGCACGG AGGGTAAAGG CGAGTACACC ATGGACTACA GCAGATATCA GCCCTGTCTG 
CCCTCTGTAC AAGAAGAGCT CGTCAACAAA CACTTGGAGG CCACGGGACA GCTGCCCGCC 
AAGAAGAGCA AGTGGAAAAA CTGA