LOC107687250 cDNA ORF clone, Sinocyclocheilus anshuiensis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107687250
Entrez Gene ID	107687250
Full Name	tyrosine-protein kinase receptor Tie-2-like
Gene Type	protein-coding
Organism	Sinocyclocheilus anshuiensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_016484503.1	XP_016339989.1	tyrosine-protein kinase receptor Tie-2-like
XM_016484502.1	XP_016339988.1	tyrosine-protein kinase receptor Tie-2-like

Pathways from BioSystems
Gene Ontology

The following LOC107687250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107687250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi105725	XM_016484503.1 Latest version!	Sinocyclocheilus anshuiensis tyrosine-protein kinase receptor Tie-2-like (LOC107687250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSi105725	XM_016484502.1 Latest version!	Sinocyclocheilus anshuiensis tyrosine-protein kinase receptor Tie-2-like (LOC107687250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107687250 ( XM_016484503.1 ) cDNA ORF clone, Sinocyclocheilus anshuiensis -> XP_016339989.1 Sinocyclocheilus anshuiensis tyrosine-protein kinase receptor Tie-2-like (LOC107687250), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSi105725
Clone ID Related Accession (Same CDS sequence)	XM_016484503.1 , XM_016484502.1
Accession Version	XM_016484503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus anshuiensis
Product	tyrosine-protein kinase receptor Tie-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015540128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus anshuiensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCACC TGTCCTCCAT CACGCTGTGT CTGATTGGCT GCTTGATCAC AGGCAGAGCT 
CTTGACATCT CAGACGTGAC TCTGATTAAT CCGGACCCGG TCGTGTCCTC GGTGAACTTG 
CCATCTCTCC GGTGTGTGAG CAGTGACTGG ACCGTTTCTG GCACCGGATC GCTGAGTTTG 
GGCCACGAGT ATCCTGATCA GGAAGCTCAT GTCCTGAGCA CTGAACCGGA CAGAAGCCAC 
CACTCTGCCG CTAAAATCAC CTGGATGTCC CGCAATCACA CCTTTGGAGC TTTTTACTGC 
CAGCTCAAAA ACTCTAGCAG GAGTAAAATC TACACCTACA AGATGCTCCA TGAAGCTGCT 
TTCCTCCCTG AATCTCTGAC AATCACTGTT AATGAAGGCG AAGACGTTAA CATCTCTTAC 
TCGAGAAAGA CATATTTACC AGAGGATACA GTCATCTATA AAAACGGAAA TTTTGAACAC 
TCGTCACCCA AAGAGGACAT CATGGATGTA ATTAATTACC CAATAATCAA TGCAAAAGCC 
CAAAGTCATG CTGGTATATA TGCGATCCGT TACATTTCTG AAGCTCCATT CAGCTCAGCA 
ATAACCCGAC TGATCGTCAG GAGTTGTAAG GCTGGTTTAT GGGGAGCAGA TTGCATGAAA 
TCGTGTCTGT CCTGTGCTAA CGGAGGCGTG TGTGATGATG TGACGGGTGA ATGCATCTGC 
CCTCCGGGGT TTAAAGGGCC CACCTGTGAA ACAGTGTGCG GTGAGGGCCG CTTCGGCCGG 
AGCTGTAAGG AGCGCTGTGT GGATGGAGTC TGTCGTTCTC TGCTCTTCTG TCTCAGAGAC 
CCGTACGGCT GCTCCTGCGC GTCTGGATGG AGGGGCTTTA ACTGCAGCGA GGCCTGTCCG 
GCCGGTTATT ATGGCGCCGA CTGTAAACAG AAGTGTGAGT GTGAACAGAG CAAATGCAGT 
CGCTTCAGAG GATGTGTGTG TGAAGGCAGG CACGGAGCTC GCTGTCAGAA ACCAGACACG 
AGACCTGTTA TCGTGAGCAG TCTGAGAGAC ATCGAGCTCA ACTCAGGCAT CACATACACA 
GTCAACTGCT CTGCGTCAGG CCAGCCCGCT CCTTTACACG GGGAAATAAC CCTGGTCAAA 
CCGGACAAAA CCACTGTCTA TGCAGTGGAC ACACAGACGG AAAATGATCA AACGACGTCA 
GCATTCAGGG TGGAGAAGAT CACTGTGTCG GCGGCTGGAC GCTGGTCGTG TCAAGTGAAA 
ACCAAACACT ATCAGGAGGA GAAAGAATTC ATTGTGAATG TCAAAGTTCC TCCGCAGCCC 
CAGAATCCTC CGCTCTTGAA TGACAGCGGC CCGCATCACC TGCTGCTGCT GCTCAATAAA 
GAGCCCTACG CCGGAGACGG GCCTGTCACC TCAGTCAAGC TCATCTACAA ACAGAACAGC 
TCATCTACAT GGAGAAGCAT TGAGGCGAGC GGCTCTCTGG TGCGGTTGGA CAACCTTTCT 
CCCATGACCC AATACACCGC ACAGGTGCAG CTGAGTCGCC ACGGACTGGG AGGAACCGGC 
CAGCCTGGAC CCGAAGCCAT TTTCTCCACA CAGATGCTCG AGGTCCCTGT GGGTGTGAAG 
CTGAGCCCCA TCAGTCAGAC CGTGCTGCTC CTGTCATGGG ACGCCGCTCC TGCTGAGGAG 
GACTGGAGTT ACGAGGTCAC GTGTCAGCAG GTCGGAGCTT CGGGAACCGA GAGGAGATTC 
CAGCTCGCGT CGAACTCCTC CGACCTCCAT CTGAACGAGC TGAAACCCAG ACACAAATAC 
CAGTGCAACG TGATGACGAG CAGCAGTGCC GCGGGACAGG CCTGCCCCAC CGTCAGCGCC 
TGGACACTGA GCGACCAGCT TCCTCCTCCT CCTGCCAACA TCTCCACCTG TAACATCAGC 
GACTCCTCAG CCGTCATCAC CTGGTTGGTG GCCGAGGGTC ACTCCATCTC CAGAGCCATG 
ATCCGCTACC AGCAGACGGA CTACAGTCAG CAGGTGGAGC TCGGCGTGCA GGACCAGACC 
AACATGCACT TCCAGCTCAG GGGTCTGAGG GAGGACACCA CCTACCAGCT GGAGCTCTGG 
AGCGTCAACA ACATGGGAGA GAGTGTGGAG AGACCGCAGA TCACCCTCAA GACACTGATG 
CCACAGGAGA GCTCACAGCT GCACAGGTTC CAGGCTCACG GTAACATGCT GTTCTACGCG 
ATCCTGGGCT CCGCCGGCAT GACGTGCATC ACTATCCTGC TGGCCTTCTG CATCGTCCTG 
CAGCTCAAGA GAGCCACTTT CCAGAGACAC ATGGTGCAGG CCTTCCACAA CATAGTGAGA 
GAGGAGCCTG TGGTCCAGTT TAGCTCCATG CCGCTGAACG TGCCCAAAAA AGTTCCCAGT 
TCCAGCCAAT CTGTGGCCTA TCCCACCCTG GAGTGGAGCG ACATTAAGTT CCAGGATGTG 
ATCGGAGAGG GAAACTTTGG GCAGGTGCTG AAAGCCCGCA TTAAGAAAGA TGGCCTGAGG 
ATGGACGCGG CCATCAAGAG AATGAAAGAA TATGCCTCTA AGGACGACCA CAGAGACTTT 
GCCGGGGAAC TCGAGGTCTT GTGTAAACTG GGTCATCATC CGAACATCAT TAACCTTCTG 
GGAGCGTGTG AACACAGAGG ATACCTGTAT TTGGCCATTG AATATGCTCC TCACGGAAAC 
CTGCTGGACT TTCTGCGTAA GAGCCGCGTT TTAGAGACGG ACCCTGCCTT TGCCATCGCT 
AACAGCACCG CATCCACGCT CTCCTCGCAG CAGTTACTGC ACTTTGCTGC AGATGTGGCG 
CATGGCATGG ACTACCTGAG CCAGAAACAG TTTATCCACA GAGATCTAGC AGCCAGAAAC 
ATCCTGGTGG GAGAAAACTT TGTGGCAAAG ATTGCTGATT TCGGCCTGTC CCGAGGTCAG 
GAGGTTTACG TGAAGAAGAC TATGGGGCAT CTGCCAGTGA GATGGATGGC CATCGAATCT 
CTCAACTACA GCGTTTACAC CACCAACAGT GATGTGTGGA ACACCGTATT GCGGTATGAC 
GTGCGCGCAG CTGTACGAGA AACTCCCGCA GGGCTATCGG CTGGAGAAGC CGCTCAACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016339989.1
CDS	104..3226
Translation	MRHLSSITLCLIGCLITGRALDISDVTLINPDPVVSSVNLPSLRCVSSDWTVSGTGSLSLGHEYPDQEAHVLSTEPDRSHHSAAKITWMSRNHTFGAFYCQLKNSSRSKIYTYKMLHEAAFLPESLTITVNEGEDVNISYSRKTYLPEDTVIYKNGNFEHSSPKEDIMDVINYPIINAKAQSHAGIYAIRYISEAPFSSAITRLIVRSCKAGLWGADCMKSCLSCANGGVCDDVTGECICPPGFKGPTCETVCGEGRFGRSCKERCVDGVCRSLLFCLRDPYGCSCASGWRGFNCSEACPAGYYGADCKQKCECEQSKCSRFRGCVCEGRHGARCQKPDTRPVIVSSLRDIELNSGITYTVNCSASGQPAPLHGEITLVKPDKTTVYAVDTQTENDQTTSAFRVEKITVSAAGRWSCQVKTKHYQEEKEFIVNVKVPPQPQNPPLLNDSGPHHLLLLLNKEPYAGDGPVTSVKLIYKQNSSSTWRSIEASGSLVRLDNLSPMTQYTAQVQLSRHGLGGTGQPGPEAIFSTQMLEVPVGVKLSPISQTVLLLSWDAAPAEEDWSYEVTCQQVGASGTERRFQLASNSSDLHLNELKPRHKYQCNVMTSSSAAGQACPTVSAWTLSDQLPPPPANISTCNISDSSAVITWLVAEGHSISRAMIRYQQTDYSQQVELGVQDQTNMHFQLRGLREDTTYQLELWSVNNMGESVERPQITLKTLMPQESSQLHRFQAHGNMLFYAILGSAGMTCITILLAFCIVLQLKRATFQRHMVQAFHNIVREEPVVQFSSMPLNVPKKVPSSSQSVAYPTLEWSDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSQQLLHFAADVAHGMDYLSQKQFIHRDLAARNILVGENFVAKIADFGLSRGQEVYVKKTMGHLPVRWMAIESLNYSVYTTNSDVWNTVLRYDVRAAVRETPAGLSAGEAAQL

Target ORF information:

RefSeq Version	XM_016484503.1
Organism	Sinocyclocheilus anshuiensis
Definition	Sinocyclocheilus anshuiensis tyrosine-protein kinase receptor Tie-2-like (LOC107687250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016484503.1

ORF Insert Sequence:

ATGAGGCACC TGTCCTCCAT CACGCTGTGT CTGATTGGCT GCTTGATCAC AGGCAGAGCT 
CTTGACATCT CAGACGTGAC TCTGATTAAT CCGGACCCGG TCGTGTCCTC GGTGAACTTG 
CCATCTCTCC GGTGTGTGAG CAGTGACTGG ACCGTTTCTG GCACCGGATC GCTGAGTTTG 
GGCCACGAGT ATCCTGATCA GGAAGCTCAT GTCCTGAGCA CTGAACCGGA CAGAAGCCAC 
CACTCTGCCG CTAAAATCAC CTGGATGTCC CGCAATCACA CCTTTGGAGC TTTTTACTGC 
CAGCTCAAAA ACTCTAGCAG GAGTAAAATC TACACCTACA AGATGCTCCA TGAAGCTGCT 
TTCCTCCCTG AATCTCTGAC AATCACTGTT AATGAAGGCG AAGACGTTAA CATCTCTTAC 
TCGAGAAAGA CATATTTACC AGAGGATACA GTCATCTATA AAAACGGAAA TTTTGAACAC 
TCGTCACCCA AAGAGGACAT CATGGATGTA ATTAATTACC CAATAATCAA TGCAAAAGCC 
CAAAGTCATG CTGGTATATA TGCGATCCGT TACATTTCTG AAGCTCCATT CAGCTCAGCA 
ATAACCCGAC TGATCGTCAG GAGTTGTAAG GCTGGTTTAT GGGGAGCAGA TTGCATGAAA 
TCGTGTCTGT CCTGTGCTAA CGGAGGCGTG TGTGATGATG TGACGGGTGA ATGCATCTGC 
CCTCCGGGGT TTAAAGGGCC CACCTGTGAA ACAGTGTGCG GTGAGGGCCG CTTCGGCCGG 
AGCTGTAAGG AGCGCTGTGT GGATGGAGTC TGTCGTTCTC TGCTCTTCTG TCTCAGAGAC 
CCGTACGGCT GCTCCTGCGC GTCTGGATGG AGGGGCTTTA ACTGCAGCGA GGCCTGTCCG 
GCCGGTTATT ATGGCGCCGA CTGTAAACAG AAGTGTGAGT GTGAACAGAG CAAATGCAGT 
CGCTTCAGAG GATGTGTGTG TGAAGGCAGG CACGGAGCTC GCTGTCAGAA ACCAGACACG 
AGACCTGTTA TCGTGAGCAG TCTGAGAGAC ATCGAGCTCA ACTCAGGCAT CACATACACA 
GTCAACTGCT CTGCGTCAGG CCAGCCCGCT CCTTTACACG GGGAAATAAC CCTGGTCAAA 
CCGGACAAAA CCACTGTCTA TGCAGTGGAC ACACAGACGG AAAATGATCA AACGACGTCA 
GCATTCAGGG TGGAGAAGAT CACTGTGTCG GCGGCTGGAC GCTGGTCGTG TCAAGTGAAA 
ACCAAACACT ATCAGGAGGA GAAAGAATTC ATTGTGAATG TCAAAGTTCC TCCGCAGCCC 
CAGAATCCTC CGCTCTTGAA TGACAGCGGC CCGCATCACC TGCTGCTGCT GCTCAATAAA 
GAGCCCTACG CCGGAGACGG GCCTGTCACC TCAGTCAAGC TCATCTACAA ACAGAACAGC 
TCATCTACAT GGAGAAGCAT TGAGGCGAGC GGCTCTCTGG TGCGGTTGGA CAACCTTTCT 
CCCATGACCC AATACACCGC ACAGGTGCAG CTGAGTCGCC ACGGACTGGG AGGAACCGGC 
CAGCCTGGAC CCGAAGCCAT TTTCTCCACA CAGATGCTCG AGGTCCCTGT GGGTGTGAAG 
CTGAGCCCCA TCAGTCAGAC CGTGCTGCTC CTGTCATGGG ACGCCGCTCC TGCTGAGGAG 
GACTGGAGTT ACGAGGTCAC GTGTCAGCAG GTCGGAGCTT CGGGAACCGA GAGGAGATTC 
CAGCTCGCGT CGAACTCCTC CGACCTCCAT CTGAACGAGC TGAAACCCAG ACACAAATAC 
CAGTGCAACG TGATGACGAG CAGCAGTGCC GCGGGACAGG CCTGCCCCAC CGTCAGCGCC 
TGGACACTGA GCGACCAGCT TCCTCCTCCT CCTGCCAACA TCTCCACCTG TAACATCAGC 
GACTCCTCAG CCGTCATCAC CTGGTTGGTG GCCGAGGGTC ACTCCATCTC CAGAGCCATG 
ATCCGCTACC AGCAGACGGA CTACAGTCAG CAGGTGGAGC TCGGCGTGCA GGACCAGACC 
AACATGCACT TCCAGCTCAG GGGTCTGAGG GAGGACACCA CCTACCAGCT GGAGCTCTGG 
AGCGTCAACA ACATGGGAGA GAGTGTGGAG AGACCGCAGA TCACCCTCAA GACACTGATG 
CCACAGGAGA GCTCACAGCT GCACAGGTTC CAGGCTCACG GTAACATGCT GTTCTACGCG 
ATCCTGGGCT CCGCCGGCAT GACGTGCATC ACTATCCTGC TGGCCTTCTG CATCGTCCTG 
CAGCTCAAGA GAGCCACTTT CCAGAGACAC ATGGTGCAGG CCTTCCACAA CATAGTGAGA 
GAGGAGCCTG TGGTCCAGTT TAGCTCCATG CCGCTGAACG TGCCCAAAAA AGTTCCCAGT 
TCCAGCCAAT CTGTGGCCTA TCCCACCCTG GAGTGGAGCG ACATTAAGTT CCAGGATGTG 
ATCGGAGAGG GAAACTTTGG GCAGGTGCTG AAAGCCCGCA TTAAGAAAGA TGGCCTGAGG 
ATGGACGCGG CCATCAAGAG AATGAAAGAA TATGCCTCTA AGGACGACCA CAGAGACTTT 
GCCGGGGAAC TCGAGGTCTT GTGTAAACTG GGTCATCATC CGAACATCAT TAACCTTCTG 
GGAGCGTGTG AACACAGAGG ATACCTGTAT TTGGCCATTG AATATGCTCC TCACGGAAAC 
CTGCTGGACT TTCTGCGTAA GAGCCGCGTT TTAGAGACGG ACCCTGCCTT TGCCATCGCT 
AACAGCACCG CATCCACGCT CTCCTCGCAG CAGTTACTGC ACTTTGCTGC AGATGTGGCG 
CATGGCATGG ACTACCTGAG CCAGAAACAG TTTATCCACA GAGATCTAGC AGCCAGAAAC 
ATCCTGGTGG GAGAAAACTT TGTGGCAAAG ATTGCTGATT TCGGCCTGTC CCGAGGTCAG 
GAGGTTTACG TGAAGAAGAC TATGGGGCAT CTGCCAGTGA GATGGATGGC CATCGAATCT 
CTCAACTACA GCGTTTACAC CACCAACAGT GATGTGTGGA ACACCGTATT GCGGTATGAC 
GTGCGCGCAG CTGTACGAGA AACTCCCGCA GGGCTATCGG CTGGAGAAGC CGCTCAACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107687250 ( XM_016484502.1 ) cDNA ORF clone, Sinocyclocheilus anshuiensis -> XP_016339988.1 Sinocyclocheilus anshuiensis tyrosine-protein kinase receptor Tie-2-like (LOC107687250), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSi105725
Clone ID Related Accession (Same CDS sequence)	XM_016484503.1 , XM_016484502.1
Accession Version	XM_016484502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus anshuiensis
Product	tyrosine-protein kinase receptor Tie-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015540128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus anshuiensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCACC TGTCCTCCAT CACGCTGTGT CTGATTGGCT GCTTGATCAC AGGCAGAGCT 
CTTGACATCT CAGACGTGAC TCTGATTAAT CCGGACCCGG TCGTGTCCTC GGTGAACTTG 
CCATCTCTCC GGTGTGTGAG CAGTGACTGG ACCGTTTCTG GCACCGGATC GCTGAGTTTG 
GGCCACGAGT ATCCTGATCA GGAAGCTCAT GTCCTGAGCA CTGAACCGGA CAGAAGCCAC 
CACTCTGCCG CTAAAATCAC CTGGATGTCC CGCAATCACA CCTTTGGAGC TTTTTACTGC 
CAGCTCAAAA ACTCTAGCAG GAGTAAAATC TACACCTACA AGATGCTCCA TGAAGCTGCT 
TTCCTCCCTG AATCTCTGAC AATCACTGTT AATGAAGGCG AAGACGTTAA CATCTCTTAC 
TCGAGAAAGA CATATTTACC AGAGGATACA GTCATCTATA AAAACGGAAA TTTTGAACAC 
TCGTCACCCA AAGAGGACAT CATGGATGTA ATTAATTACC CAATAATCAA TGCAAAAGCC 
CAAAGTCATG CTGGTATATA TGCGATCCGT TACATTTCTG AAGCTCCATT CAGCTCAGCA 
ATAACCCGAC TGATCGTCAG GAGTTGTAAG GCTGGTTTAT GGGGAGCAGA TTGCATGAAA 
TCGTGTCTGT CCTGTGCTAA CGGAGGCGTG TGTGATGATG TGACGGGTGA ATGCATCTGC 
CCTCCGGGGT TTAAAGGGCC CACCTGTGAA ACAGTGTGCG GTGAGGGCCG CTTCGGCCGG 
AGCTGTAAGG AGCGCTGTGT GGATGGAGTC TGTCGTTCTC TGCTCTTCTG TCTCAGAGAC 
CCGTACGGCT GCTCCTGCGC GTCTGGATGG AGGGGCTTTA ACTGCAGCGA GGCCTGTCCG 
GCCGGTTATT ATGGCGCCGA CTGTAAACAG AAGTGTGAGT GTGAACAGAG CAAATGCAGT 
CGCTTCAGAG GATGTGTGTG TGAAGGCAGG CACGGAGCTC GCTGTCAGAA ACCAGACACG 
AGACCTGTTA TCGTGAGCAG TCTGAGAGAC ATCGAGCTCA ACTCAGGCAT CACATACACA 
GTCAACTGCT CTGCGTCAGG CCAGCCCGCT CCTTTACACG GGGAAATAAC CCTGGTCAAA 
CCGGACAAAA CCACTGTCTA TGCAGTGGAC ACACAGACGG AAAATGATCA AACGACGTCA 
GCATTCAGGG TGGAGAAGAT CACTGTGTCG GCGGCTGGAC GCTGGTCGTG TCAAGTGAAA 
ACCAAACACT ATCAGGAGGA GAAAGAATTC ATTGTGAATG TCAAAGTTCC TCCGCAGCCC 
CAGAATCCTC CGCTCTTGAA TGACAGCGGC CCGCATCACC TGCTGCTGCT GCTCAATAAA 
GAGCCCTACG CCGGAGACGG GCCTGTCACC TCAGTCAAGC TCATCTACAA ACAGAACAGC 
TCATCTACAT GGAGAAGCAT TGAGGCGAGC GGCTCTCTGG TGCGGTTGGA CAACCTTTCT 
CCCATGACCC AATACACCGC ACAGGTGCAG CTGAGTCGCC ACGGACTGGG AGGAACCGGC 
CAGCCTGGAC CCGAAGCCAT TTTCTCCACA CAGATGCTCG AGGTCCCTGT GGGTGTGAAG 
CTGAGCCCCA TCAGTCAGAC CGTGCTGCTC CTGTCATGGG ACGCCGCTCC TGCTGAGGAG 
GACTGGAGTT ACGAGGTCAC GTGTCAGCAG GTCGGAGCTT CGGGAACCGA GAGGAGATTC 
CAGCTCGCGT CGAACTCCTC CGACCTCCAT CTGAACGAGC TGAAACCCAG ACACAAATAC 
CAGTGCAACG TGATGACGAG CAGCAGTGCC GCGGGACAGG CCTGCCCCAC CGTCAGCGCC 
TGGACACTGA GCGACCAGCT TCCTCCTCCT CCTGCCAACA TCTCCACCTG TAACATCAGC 
GACTCCTCAG CCGTCATCAC CTGGTTGGTG GCCGAGGGTC ACTCCATCTC CAGAGCCATG 
ATCCGCTACC AGCAGACGGA CTACAGTCAG CAGGTGGAGC TCGGCGTGCA GGACCAGACC 
AACATGCACT TCCAGCTCAG GGGTCTGAGG GAGGACACCA CCTACCAGCT GGAGCTCTGG 
AGCGTCAACA ACATGGGAGA GAGTGTGGAG AGACCGCAGA TCACCCTCAA GACACTGATG 
CCACAGGAGA GCTCACAGCT GCACAGGTTC CAGGCTCACG GTAACATGCT GTTCTACGCG 
ATCCTGGGCT CCGCCGGCAT GACGTGCATC ACTATCCTGC TGGCCTTCTG CATCGTCCTG 
CAGCTCAAGA GAGCCACTTT CCAGAGACAC ATGGTGCAGG CCTTCCACAA CATAGTGAGA 
GAGGAGCCTG TGGTCCAGTT TAGCTCCATG CCGCTGAACG TGCCCAAAAA AGTTCCCAGT 
TCCAGCCAAT CTGTGGCCTA TCCCACCCTG GAGTGGAGCG ACATTAAGTT CCAGGATGTG 
ATCGGAGAGG GAAACTTTGG GCAGGTGCTG AAAGCCCGCA TTAAGAAAGA TGGCCTGAGG 
ATGGACGCGG CCATCAAGAG AATGAAAGAA TATGCCTCTA AGGACGACCA CAGAGACTTT 
GCCGGGGAAC TCGAGGTCTT GTGTAAACTG GGTCATCATC CGAACATCAT TAACCTTCTG 
GGAGCGTGTG AACACAGAGG ATACCTGTAT TTGGCCATTG AATATGCTCC TCACGGAAAC 
CTGCTGGACT TTCTGCGTAA GAGCCGCGTT TTAGAGACGG ACCCTGCCTT TGCCATCGCT 
AACAGCACCG CATCCACGCT CTCCTCGCAG CAGTTACTGC ACTTTGCTGC AGATGTGGCG 
CATGGCATGG ACTACCTGAG CCAGAAACAG TTTATCCACA GAGATCTAGC AGCCAGAAAC 
ATCCTGGTGG GAGAAAACTT TGTGGCAAAG ATTGCTGATT TCGGCCTGTC CCGAGGTCAG 
GAGGTTTACG TGAAGAAGAC TATGGGGCAT CTGCCAGTGA GATGGATGGC CATCGAATCT 
CTCAACTACA GCGTTTACAC CACCAACAGT GATGTGTGGA ACACCGTATT GCGGTATGAC 
GTGCGCGCAG CTGTACGAGA AACTCCCGCA GGGCTATCGG CTGGAGAAGC CGCTCAACTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016339988.1
CDS	104..3226
Translation	MRHLSSITLCLIGCLITGRALDISDVTLINPDPVVSSVNLPSLRCVSSDWTVSGTGSLSLGHEYPDQEAHVLSTEPDRSHHSAAKITWMSRNHTFGAFYCQLKNSSRSKIYTYKMLHEAAFLPESLTITVNEGEDVNISYSRKTYLPEDTVIYKNGNFEHSSPKEDIMDVINYPIINAKAQSHAGIYAIRYISEAPFSSAITRLIVRSCKAGLWGADCMKSCLSCANGGVCDDVTGECICPPGFKGPTCETVCGEGRFGRSCKERCVDGVCRSLLFCLRDPYGCSCASGWRGFNCSEACPAGYYGADCKQKCECEQSKCSRFRGCVCEGRHGARCQKPDTRPVIVSSLRDIELNSGITYTVNCSASGQPAPLHGEITLVKPDKTTVYAVDTQTENDQTTSAFRVEKITVSAAGRWSCQVKTKHYQEEKEFIVNVKVPPQPQNPPLLNDSGPHHLLLLLNKEPYAGDGPVTSVKLIYKQNSSSTWRSIEASGSLVRLDNLSPMTQYTAQVQLSRHGLGGTGQPGPEAIFSTQMLEVPVGVKLSPISQTVLLLSWDAAPAEEDWSYEVTCQQVGASGTERRFQLASNSSDLHLNELKPRHKYQCNVMTSSSAAGQACPTVSAWTLSDQLPPPPANISTCNISDSSAVITWLVAEGHSISRAMIRYQQTDYSQQVELGVQDQTNMHFQLRGLREDTTYQLELWSVNNMGESVERPQITLKTLMPQESSQLHRFQAHGNMLFYAILGSAGMTCITILLAFCIVLQLKRATFQRHMVQAFHNIVREEPVVQFSSMPLNVPKKVPSSSQSVAYPTLEWSDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSQQLLHFAADVAHGMDYLSQKQFIHRDLAARNILVGENFVAKIADFGLSRGQEVYVKKTMGHLPVRWMAIESLNYSVYTTNSDVWNTVLRYDVRAAVRETPAGLSAGEAAQL

Target ORF information:

RefSeq Version	XM_016484502.1
Organism	Sinocyclocheilus anshuiensis
Definition	Sinocyclocheilus anshuiensis tyrosine-protein kinase receptor Tie-2-like (LOC107687250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016484502.1

ORF Insert Sequence:

ATGAGGCACC TGTCCTCCAT CACGCTGTGT CTGATTGGCT GCTTGATCAC AGGCAGAGCT 
CTTGACATCT CAGACGTGAC TCTGATTAAT CCGGACCCGG TCGTGTCCTC GGTGAACTTG 
CCATCTCTCC GGTGTGTGAG CAGTGACTGG ACCGTTTCTG GCACCGGATC GCTGAGTTTG 
GGCCACGAGT ATCCTGATCA GGAAGCTCAT GTCCTGAGCA CTGAACCGGA CAGAAGCCAC 
CACTCTGCCG CTAAAATCAC CTGGATGTCC CGCAATCACA CCTTTGGAGC TTTTTACTGC 
CAGCTCAAAA ACTCTAGCAG GAGTAAAATC TACACCTACA AGATGCTCCA TGAAGCTGCT 
TTCCTCCCTG AATCTCTGAC AATCACTGTT AATGAAGGCG AAGACGTTAA CATCTCTTAC 
TCGAGAAAGA CATATTTACC AGAGGATACA GTCATCTATA AAAACGGAAA TTTTGAACAC 
TCGTCACCCA AAGAGGACAT CATGGATGTA ATTAATTACC CAATAATCAA TGCAAAAGCC 
CAAAGTCATG CTGGTATATA TGCGATCCGT TACATTTCTG AAGCTCCATT CAGCTCAGCA 
ATAACCCGAC TGATCGTCAG GAGTTGTAAG GCTGGTTTAT GGGGAGCAGA TTGCATGAAA 
TCGTGTCTGT CCTGTGCTAA CGGAGGCGTG TGTGATGATG TGACGGGTGA ATGCATCTGC 
CCTCCGGGGT TTAAAGGGCC CACCTGTGAA ACAGTGTGCG GTGAGGGCCG CTTCGGCCGG 
AGCTGTAAGG AGCGCTGTGT GGATGGAGTC TGTCGTTCTC TGCTCTTCTG TCTCAGAGAC 
CCGTACGGCT GCTCCTGCGC GTCTGGATGG AGGGGCTTTA ACTGCAGCGA GGCCTGTCCG 
GCCGGTTATT ATGGCGCCGA CTGTAAACAG AAGTGTGAGT GTGAACAGAG CAAATGCAGT 
CGCTTCAGAG GATGTGTGTG TGAAGGCAGG CACGGAGCTC GCTGTCAGAA ACCAGACACG 
AGACCTGTTA TCGTGAGCAG TCTGAGAGAC ATCGAGCTCA ACTCAGGCAT CACATACACA 
GTCAACTGCT CTGCGTCAGG CCAGCCCGCT CCTTTACACG GGGAAATAAC CCTGGTCAAA 
CCGGACAAAA CCACTGTCTA TGCAGTGGAC ACACAGACGG AAAATGATCA AACGACGTCA 
GCATTCAGGG TGGAGAAGAT CACTGTGTCG GCGGCTGGAC GCTGGTCGTG TCAAGTGAAA 
ACCAAACACT ATCAGGAGGA GAAAGAATTC ATTGTGAATG TCAAAGTTCC TCCGCAGCCC 
CAGAATCCTC CGCTCTTGAA TGACAGCGGC CCGCATCACC TGCTGCTGCT GCTCAATAAA 
GAGCCCTACG CCGGAGACGG GCCTGTCACC TCAGTCAAGC TCATCTACAA ACAGAACAGC 
TCATCTACAT GGAGAAGCAT TGAGGCGAGC GGCTCTCTGG TGCGGTTGGA CAACCTTTCT 
CCCATGACCC AATACACCGC ACAGGTGCAG CTGAGTCGCC ACGGACTGGG AGGAACCGGC 
CAGCCTGGAC CCGAAGCCAT TTTCTCCACA CAGATGCTCG AGGTCCCTGT GGGTGTGAAG 
CTGAGCCCCA TCAGTCAGAC CGTGCTGCTC CTGTCATGGG ACGCCGCTCC TGCTGAGGAG 
GACTGGAGTT ACGAGGTCAC GTGTCAGCAG GTCGGAGCTT CGGGAACCGA GAGGAGATTC 
CAGCTCGCGT CGAACTCCTC CGACCTCCAT CTGAACGAGC TGAAACCCAG ACACAAATAC 
CAGTGCAACG TGATGACGAG CAGCAGTGCC GCGGGACAGG CCTGCCCCAC CGTCAGCGCC 
TGGACACTGA GCGACCAGCT TCCTCCTCCT CCTGCCAACA TCTCCACCTG TAACATCAGC 
GACTCCTCAG CCGTCATCAC CTGGTTGGTG GCCGAGGGTC ACTCCATCTC CAGAGCCATG 
ATCCGCTACC AGCAGACGGA CTACAGTCAG CAGGTGGAGC TCGGCGTGCA GGACCAGACC 
AACATGCACT TCCAGCTCAG GGGTCTGAGG GAGGACACCA CCTACCAGCT GGAGCTCTGG 
AGCGTCAACA ACATGGGAGA GAGTGTGGAG AGACCGCAGA TCACCCTCAA GACACTGATG 
CCACAGGAGA GCTCACAGCT GCACAGGTTC CAGGCTCACG GTAACATGCT GTTCTACGCG 
ATCCTGGGCT CCGCCGGCAT GACGTGCATC ACTATCCTGC TGGCCTTCTG CATCGTCCTG 
CAGCTCAAGA GAGCCACTTT CCAGAGACAC ATGGTGCAGG CCTTCCACAA CATAGTGAGA 
GAGGAGCCTG TGGTCCAGTT TAGCTCCATG CCGCTGAACG TGCCCAAAAA AGTTCCCAGT 
TCCAGCCAAT CTGTGGCCTA TCCCACCCTG GAGTGGAGCG ACATTAAGTT CCAGGATGTG 
ATCGGAGAGG GAAACTTTGG GCAGGTGCTG AAAGCCCGCA TTAAGAAAGA TGGCCTGAGG 
ATGGACGCGG CCATCAAGAG AATGAAAGAA TATGCCTCTA AGGACGACCA CAGAGACTTT 
GCCGGGGAAC TCGAGGTCTT GTGTAAACTG GGTCATCATC CGAACATCAT TAACCTTCTG 
GGAGCGTGTG AACACAGAGG ATACCTGTAT TTGGCCATTG AATATGCTCC TCACGGAAAC 
CTGCTGGACT TTCTGCGTAA GAGCCGCGTT TTAGAGACGG ACCCTGCCTT TGCCATCGCT 
AACAGCACCG CATCCACGCT CTCCTCGCAG CAGTTACTGC ACTTTGCTGC AGATGTGGCG 
CATGGCATGG ACTACCTGAG CCAGAAACAG TTTATCCACA GAGATCTAGC AGCCAGAAAC 
ATCCTGGTGG GAGAAAACTT TGTGGCAAAG ATTGCTGATT TCGGCCTGTC CCGAGGTCAG 
GAGGTTTACG TGAAGAAGAC TATGGGGCAT CTGCCAGTGA GATGGATGGC CATCGAATCT 
CTCAACTACA GCGTTTACAC CACCAACAGT GATGTGTGGA ACACCGTATT GCGGTATGAC 
GTGCGCGCAG CTGTACGAGA AACTCCCGCA GGGCTATCGG CTGGAGAAGC CGCTCAACTG 
TGA