cdc42bpa cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc42bpa
Entrez Gene ID	108924070
Full Name	CDC42 binding protein kinase alpha
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029251335.1	XP_029107168.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_029251326.1	XP_029107159.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_018735190.1	XP_018590706.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_018735192.1	XP_018590708.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_018735191.1	XP_018590707.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_029251331.1	XP_029107164.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_029251333.1	XP_029107166.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_018735192.2	XP_018590708.2	serine/threonine-protein kinase MRCK alpha isoform X4

Pathways from BioSystems
Gene Ontology

The following cdc42bpa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc42bpa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc139148	XM_029251326.1 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc139148	XM_029251331.1 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc139149	XM_029251333.1 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc139150	XM_029251335.1 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc139151	XM_018735192.2 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc41989	XM_018735190.1 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSc41990	XM_018735191.1 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSc41991	XM_018735192.1 Latest version!	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc42bpa ( XM_029251326.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029107159.1 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSc139148
Clone ID Related Accession (Same CDS sequence)	XM_029251326.1 , XM_029251331.1
Accession Version	XM_029251326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGTGGCAGGC TTCAGAGAAG ACCCCTGATC TCAAAAGGGC ATTAAGGACC 
CTGCTTAGTA AGATGAAGGT AATATTGACA TGGCTGTGTG CGCTCACACA CCTGAGCTCG 
TGTTCCAGCA GCTGTGCCCC CACCCCCATC CCCTGCCATG CCTCCACCCC CATCCCCTGC 
CATGCCTCAC TTTGGACAAC TGTTAGAGTC TCAGTTTCAG TTATAGTCAG TAAGCCCACA 
TGTACTGCAG TGGAACGGAG CCTCTTCAAA GGCGTGTTCT TCTGTCTCCT CCTTGCTGGA 
GGTGTTCAGA GAGCACAGTG GGGAGAGTTG CCCCCCACCA TGCACACAGT GCCTTGTTTA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029107159.1
CDS	538..6063
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDRSPSCVAPSKGRRIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREWQASEKTPDLKRALRTLLSKMKVILTWLCALTHLSSCSSSCAPTPIPCHASTPIPCHASLWTTVRVSVSVIVSKPTCTAVERSLFKGVFFCLLLAGGVQRAQWGELPPTMHTVPCLA

Target ORF information:

RefSeq Version	XM_029251326.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029251326.1

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGTGGCAGGC TTCAGAGAAG ACCCCTGATC TCAAAAGGGC ATTAAGGACC 
CTGCTTAGTA AGATGAAGGT AATATTGACA TGGCTGTGTG CGCTCACACA CCTGAGCTCG 
TGTTCCAGCA GCTGTGCCCC CACCCCCATC CCCTGCCATG CCTCCACCCC CATCCCCTGC 
CATGCCTCAC TTTGGACAAC TGTTAGAGTC TCAGTTTCAG TTATAGTCAG TAAGCCCACA 
TGTACTGCAG TGGAACGGAG CCTCTTCAAA GGCGTGTTCT TCTGTCTCCT CCTTGCTGGA 
GGTGTTCAGA GAGCACAGTG GGGAGAGTTG CCCCCCACCA TGCACACAGT GCCTTGTTTA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42bpa ( XM_029251331.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029107164.1 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X2, mRNA.

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CloneID	OSc139148
Clone ID Related Accession (Same CDS sequence)	XM_029251326.1 , XM_029251331.1
Accession Version	XM_029251331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGTGGCAGGC TTCAGAGAAG ACCCCTGATC TCAAAAGGGC ATTAAGGACC 
CTGCTTAGTA AGATGAAGGT AATATTGACA TGGCTGTGTG CGCTCACACA CCTGAGCTCG 
TGTTCCAGCA GCTGTGCCCC CACCCCCATC CCCTGCCATG CCTCCACCCC CATCCCCTGC 
CATGCCTCAC TTTGGACAAC TGTTAGAGTC TCAGTTTCAG TTATAGTCAG TAAGCCCACA 
TGTACTGCAG TGGAACGGAG CCTCTTCAAA GGCGTGTTCT TCTGTCTCCT CCTTGCTGGA 
GGTGTTCAGA GAGCACAGTG GGGAGAGTTG CCCCCCACCA TGCACACAGT GCCTTGTTTA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029107164.1
CDS	512..6037
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDRSPSCVAPSKGRRIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREWQASEKTPDLKRALRTLLSKMKVILTWLCALTHLSSCSSSCAPTPIPCHASTPIPCHASLWTTVRVSVSVIVSKPTCTAVERSLFKGVFFCLLLAGGVQRAQWGELPPTMHTVPCLA

Target ORF information:

RefSeq Version	XM_029251331.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029251331.1

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGTGGCAGGC TTCAGAGAAG ACCCCTGATC TCAAAAGGGC ATTAAGGACC 
CTGCTTAGTA AGATGAAGGT AATATTGACA TGGCTGTGTG CGCTCACACA CCTGAGCTCG 
TGTTCCAGCA GCTGTGCCCC CACCCCCATC CCCTGCCATG CCTCCACCCC CATCCCCTGC 
CATGCCTCAC TTTGGACAAC TGTTAGAGTC TCAGTTTCAG TTATAGTCAG TAAGCCCACA 
TGTACTGCAG TGGAACGGAG CCTCTTCAAA GGCGTGTTCT TCTGTCTCCT CCTTGCTGGA 
GGTGTTCAGA GAGCACAGTG GGGAGAGTTG CCCCCCACCA TGCACACAGT GCCTTGTTTA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42bpa ( XM_029251333.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029107166.1 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X3, mRNA.

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CloneID	OSc139149
Clone ID Related Accession (Same CDS sequence)	XM_029251333.1
Accession Version	XM_029251333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TATTGATTCT ATGGACAACT TCACTCCTTC CCGGGAAGAT 
GAGGCCAAAT CCCGCCTGAT GTCCCGATCA CGGTCGCCAT CCACAGCCAG TGACATGGAG 
CCACCGGAGA TAATAGACCA CCATCAACGG TCTGTTCTGA CCCCCACCGT GAAACTAGGC 
TTCAGTGGGT CTAGTCTAAC AGCCAAGCCC AAAGCCCACC AGTTTGTCGT GAAGTCCTTC 
AACACTCCCA CCAAGTGCAA CCAGTGCACC TCCCTCATGG TGGGACTCAT TCGCCAGGGC 
TGCACCTGTG AAGTCTGCAA CTTCTCATGT CATGTGACGT GTGCAGACAA GGCGCCAGCT 
GTATGCCCAA TCCCACCAGA CCAGACCAAA GGTCCACTGG GCATCGACCC CCAGAAGGGC 
ATCGGCACAG CCTATGAAGG CCACGTTCGA GTGCCCAAGC CAGCAGGGGT GAAGAAAGGC 
TGGCAGCGGG CTCTTGCCGT TGTGTGTGAC TTCAAGCTGT TTCTGTATGA CCTGGCTGAA 
GGCAAGGCAT CCACCCCCAG CGTTGTGATC AGCCAGGTCA TTGACATGAG GGACGAGGAC 
TTCTCTGTCA GCTCAGTCTT GGCCTCCGAT GTTATTCATG CCAACCGGAA GGACATCCCC 
TGCATTTTCA GGGTGACAGC CTCTCAGCTG TCTGCCTCCA GCAGTCACAA GTGCTCCATC 
CTAATTCTGG CCGACAGCGA GCAGGAACGT AGCAAGTGGG TGGGGCTGCT GAACGAGATG 
CACCGTCTGC TGAGGAAGAG CAAGTTGAAG GACCGCTTTG TCTATGTGCC CAAAGAGGCC 
TATGACAGCA CGCTGCCTCT CATCAAGAGT GCGCAGTCTG CTGCCATCAT AGATCATGAG 
CGTGTTGCTC TTGGCAACGA AGAGGGTCTG TATGTCATCC ATGTCACCAA AGATGAAATT 
GTCCGGGTGG GCGACAATAA GAAGGTGCAC CACATTGAAC TGATGCCTTC AGAGCAGCTG 
CTGGCAGTCA TCTCTGGCCG CAACCGTCAC GTTCGTCTGT TCCCCATGTT GGCACTAGAT 
GGTCGTGAGA CGGACTCTTA CAAACTGGCA GAGACCAAAG GCTGCCAGAC ATTGGTGTCA 
GGCCGACCAC GGCCCGGTGG ACCCACTTGC CTTTGCGTGG CCATGAAACG GCAGGTGATT 
TGCTACGAGA TTAACAAAAG CAAGACACAT CACCGTAAGT TACGGGACTT CCAGGTGCCA 
GGTCCTGTAC AGTGGATGGC TCTCCTAGGC GACCGGCTGT GCGTAGGCTA CCAGGCTGGT 
TTTGCCCGCT ACAGCCCGCA TTCGGATGCA GTGCCTGTCA GCCTGCTCCA CCCCGAGGAC 
CCCACCCTGT CCTTCATTAG CCAGCAGGGC CTGGATGCCA TCTGTGCTGT GGAGATCTCT 
TCCAAGGAGT TCCTGCTGTG CTTCAGCTGC GTGGGTGTGT ACGTAGACTG CCAGGGCAGG 
AGGTCGCGTC AGCAGGAGCT CATGTGGCCA GCCGTGCCCA CCGCCTGCTG CTACAACGCC 
CCCTACCTGT CAGTGTACAG TGAGAATGCT GTGGATGTGT TTGACGTCAA CACCATGGAG 
TGGATCCAGA CCATTCCTTT GAAGAAGGTG CGGCCTCTCA ACACAGACGG CTCCCTCAAC 
CTGCTCGGCC TGGAGACAGT TCGGCTCATT TATTTCAAGA ACAAGATGGC AGAAGGAGAC 
GAGCTTGTAG TTCCTGAGAC ATCGGACAAC AGCCGGAAGC AGATGGTGCG CAGCATCAAC 
AATAAGAGAC GCTTTTCCTT CCGTGTACCT GAGGAAGAGA GGATGCAGCA AAGGAGAGAG 
ATGCTGAAGG ACCCAGAAAT GAGGAACAAG CTCATCTCCA ACCCCACCAA CTTTAACCAT 
GTTGTTCACA TGGGCCCAGG TGACGGCATC CAGATTCTCA AAGACCTTCC CATGAGTGTC 
CGTCCCCAGG AGAGTCGCGC TGTCTTCAGT GGCTCTGTGA GCATCCCCTC CATCGCCAAG 
TCCCGGGGCG AGCCGGGCCG CTCCATGAGT GCCAGCAGTG GTCTGGGCAC ACGGTCCACA 
GCACAGAACG GCAGTGCCCT GAGGAGGGAG TTCTCTGGTG GGAGCTATGG CTCCAAGCGC 
CAGGCCATGA CCTCTCCCTC TGAGGGATCC TTGTCCTCGG GTGGGGGGAC GGATGGTACC 
ACAGATGCTC CCATTTCACA GTTCGACAGA GAGTGGCAGG CTTCAGAGAA GACCCCTGAT 
CTCAAAAGGG CATTAAGGAC CCTGCTTAGT AAGATGAAGG TAATATTGAC ATGGCTGTGT 
GCGCTCACAC ACCTGAGCTC GTGTTCCAGC AGCTGTGCCC CCACCCCCAT CCCCTGCCAT 
GCCTCCACCC CCATCCCCTG CCATGCCTCA CTTTGGACAA CTGTTAGAGT CTCAGTTTCA 
GTTATAGTCA GTAAGCCCAC ATGTACTGCA GTGGAACGGA GCCTCTTCAA AGGCGTGTTC 
TTCTGTCTCC TCCTTGCTGG AGGTGTTCAG AGAGCACAGT GGGGAGAGTT GCCCCCCACC 
ATGCACACAG TGCCTTGTTT AGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029107166.1
CDS	877..6363
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREWQASEKTPDLKRALRTLLSKMKVILTWLCALTHLSSCSSSCAPTPIPCHASTPIPCHASLWTTVRVSVSVIVSKPTCTAVERSLFKGVFFCLLLAGGVQRAQWGELPPTMHTVPCLA

Target ORF information:

RefSeq Version	XM_029251333.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029251333.1

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TATTGATTCT ATGGACAACT TCACTCCTTC CCGGGAAGAT 
GAGGCCAAAT CCCGCCTGAT GTCCCGATCA CGGTCGCCAT CCACAGCCAG TGACATGGAG 
CCACCGGAGA TAATAGACCA CCATCAACGG TCTGTTCTGA CCCCCACCGT GAAACTAGGC 
TTCAGTGGGT CTAGTCTAAC AGCCAAGCCC AAAGCCCACC AGTTTGTCGT GAAGTCCTTC 
AACACTCCCA CCAAGTGCAA CCAGTGCACC TCCCTCATGG TGGGACTCAT TCGCCAGGGC 
TGCACCTGTG AAGTCTGCAA CTTCTCATGT CATGTGACGT GTGCAGACAA GGCGCCAGCT 
GTATGCCCAA TCCCACCAGA CCAGACCAAA GGTCCACTGG GCATCGACCC CCAGAAGGGC 
ATCGGCACAG CCTATGAAGG CCACGTTCGA GTGCCCAAGC CAGCAGGGGT GAAGAAAGGC 
TGGCAGCGGG CTCTTGCCGT TGTGTGTGAC TTCAAGCTGT TTCTGTATGA CCTGGCTGAA 
GGCAAGGCAT CCACCCCCAG CGTTGTGATC AGCCAGGTCA TTGACATGAG GGACGAGGAC 
TTCTCTGTCA GCTCAGTCTT GGCCTCCGAT GTTATTCATG CCAACCGGAA GGACATCCCC 
TGCATTTTCA GGGTGACAGC CTCTCAGCTG TCTGCCTCCA GCAGTCACAA GTGCTCCATC 
CTAATTCTGG CCGACAGCGA GCAGGAACGT AGCAAGTGGG TGGGGCTGCT GAACGAGATG 
CACCGTCTGC TGAGGAAGAG CAAGTTGAAG GACCGCTTTG TCTATGTGCC CAAAGAGGCC 
TATGACAGCA CGCTGCCTCT CATCAAGAGT GCGCAGTCTG CTGCCATCAT AGATCATGAG 
CGTGTTGCTC TTGGCAACGA AGAGGGTCTG TATGTCATCC ATGTCACCAA AGATGAAATT 
GTCCGGGTGG GCGACAATAA GAAGGTGCAC CACATTGAAC TGATGCCTTC AGAGCAGCTG 
CTGGCAGTCA TCTCTGGCCG CAACCGTCAC GTTCGTCTGT TCCCCATGTT GGCACTAGAT 
GGTCGTGAGA CGGACTCTTA CAAACTGGCA GAGACCAAAG GCTGCCAGAC ATTGGTGTCA 
GGCCGACCAC GGCCCGGTGG ACCCACTTGC CTTTGCGTGG CCATGAAACG GCAGGTGATT 
TGCTACGAGA TTAACAAAAG CAAGACACAT CACCGTAAGT TACGGGACTT CCAGGTGCCA 
GGTCCTGTAC AGTGGATGGC TCTCCTAGGC GACCGGCTGT GCGTAGGCTA CCAGGCTGGT 
TTTGCCCGCT ACAGCCCGCA TTCGGATGCA GTGCCTGTCA GCCTGCTCCA CCCCGAGGAC 
CCCACCCTGT CCTTCATTAG CCAGCAGGGC CTGGATGCCA TCTGTGCTGT GGAGATCTCT 
TCCAAGGAGT TCCTGCTGTG CTTCAGCTGC GTGGGTGTGT ACGTAGACTG CCAGGGCAGG 
AGGTCGCGTC AGCAGGAGCT CATGTGGCCA GCCGTGCCCA CCGCCTGCTG CTACAACGCC 
CCCTACCTGT CAGTGTACAG TGAGAATGCT GTGGATGTGT TTGACGTCAA CACCATGGAG 
TGGATCCAGA CCATTCCTTT GAAGAAGGTG CGGCCTCTCA ACACAGACGG CTCCCTCAAC 
CTGCTCGGCC TGGAGACAGT TCGGCTCATT TATTTCAAGA ACAAGATGGC AGAAGGAGAC 
GAGCTTGTAG TTCCTGAGAC ATCGGACAAC AGCCGGAAGC AGATGGTGCG CAGCATCAAC 
AATAAGAGAC GCTTTTCCTT CCGTGTACCT GAGGAAGAGA GGATGCAGCA AAGGAGAGAG 
ATGCTGAAGG ACCCAGAAAT GAGGAACAAG CTCATCTCCA ACCCCACCAA CTTTAACCAT 
GTTGTTCACA TGGGCCCAGG TGACGGCATC CAGATTCTCA AAGACCTTCC CATGAGTGTC 
CGTCCCCAGG AGAGTCGCGC TGTCTTCAGT GGCTCTGTGA GCATCCCCTC CATCGCCAAG 
TCCCGGGGCG AGCCGGGCCG CTCCATGAGT GCCAGCAGTG GTCTGGGCAC ACGGTCCACA 
GCACAGAACG GCAGTGCCCT GAGGAGGGAG TTCTCTGGTG GGAGCTATGG CTCCAAGCGC 
CAGGCCATGA CCTCTCCCTC TGAGGGATCC TTGTCCTCGG GTGGGGGGAC GGATGGTACC 
ACAGATGCTC CCATTTCACA GTTCGACAGA GAGTGGCAGG CTTCAGAGAA GACCCCTGAT 
CTCAAAAGGG CATTAAGGAC CCTGCTTAGT AAGATGAAGG TAATATTGAC ATGGCTGTGT 
GCGCTCACAC ACCTGAGCTC GTGTTCCAGC AGCTGTGCCC CCACCCCCAT CCCCTGCCAT 
GCCTCCACCC CCATCCCCTG CCATGCCTCA CTTTGGACAA CTGTTAGAGT CTCAGTTTCA 
GTTATAGTCA GTAAGCCCAC ATGTACTGCA GTGGAACGGA GCCTCTTCAA AGGCGTGTTC 
TTCTGTCTCC TCCTTGCTGG AGGTGTTCAG AGAGCACAGT GGGGAGAGTT GCCCCCCACC 
ATGCACACAG TGCCTTGTTT AGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42bpa ( XM_029251335.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029107168.1 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X4, mRNA.

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CloneID	OSc139150
Clone ID Related Accession (Same CDS sequence)	XM_029251335.1
Accession Version	XM_029251335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGGACTCAGA CTCCCCCCGC CACTCCACAG CATCCAACAG CTCGAACCTG 
AGCAGCCCCC CAAGCCCAGC TTCCCCACAC AAGACCAAGT CTCTGTCCCT GGAGAGCACA 
GACCGCATGG GCTGGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029107168.1
CDS	876..6179
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDRSPSCVAPSKGRRIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREDSDSPRHSTASNSSNLSSPPSPASPHKTKSLSLESTDRMGWET

Target ORF information:

RefSeq Version	XM_029251335.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029251335.1

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGGACTCAGA CTCCCCCCGC CACTCCACAG CATCCAACAG CTCGAACCTG 
AGCAGCCCCC CAAGCCCAGC TTCCCCACAC AAGACCAAGT CTCTGTCCCT GGAGAGCACA 
GACCGCATGG GCTGGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42bpa ( XM_018735192.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018590708.2 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X5, mRNA.

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CloneID	OSc139151
Clone ID Related Accession (Same CDS sequence)	XM_018735192.2
Accession Version	XM_018735192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018735192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGCCTGGTT CACCCCTGCC CTCGTGCCCC 
TCCCCACACA CACGCCTCAT CTCCACTCCC ACCAGCTTTG AGCATGTCTA TCATATGACT 
GCCCACTCGG CTGAAAACTT TCTTTCCCAA GACTGCGCCC CACTTTCCAC CTACACCCCA 
TCACTGCGCT CTTTCCCCGG GACACCTGTC TCCATCTGCT CTCGGAGTGT CCGTCCCCAG 
GAGAGTCGCG CTGTCTTCAG TGGCTCTGTG AGCATCCCCT CCATCGCCAA GTCCCGGGGC 
GAGCCGGGCC GCTCCATGAG TGCCAGCAGT GGTCTGGGCA CACGGTCCAC AGCACAGAAC 
GGCAGTGCCC TGAGGAGGGA GTTCTCTGGT GGGAGCTATG GCTCCAAGCG CCAGGCCATG 
ACCTCTCCCT CTGAGGGATC CTTGTCCTCG GGTGGGGGGA CGGATGGTAC CACAGATGCT 
CCCATTTCAC AGTTCGACAG AGAGTGGCAG GCTTCAGAGA AGACCCCTGA TCTCAAAAGG 
GCATTAAGGA CCCTGCTTAG TAAGATGAAG GACTCAGACT CCCCCCGCCA CTCCACAGCA 
TCCAACAGCT CGAACCTGAG CAGCCCCCCA AGCCCAGCTT CCCCACACAA GACCAAGTCT 
CTGTCCCTGG AGAGCACAGA CCGCATGGGC TGGGAGACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018590708.2
CDS	878..6439
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDRSPSCVAPSKGRRIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMPGSPLPSCPSPHTRLISTPTSFEHVYHMTAHSAENFLSQDCAPLSTYTPSLRSFPGTPVSICSRSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREWQASEKTPDLKRALRTLLSKMKDSDSPRHSTASNSSNLSSPPSPASPHKTKSLSLESTDRMGWET

Target ORF information:

RefSeq Version	XM_018735192.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018735192.2

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TATGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGCCTGGTT CACCCCTGCC CTCGTGCCCC 
TCCCCACACA CACGCCTCAT CTCCACTCCC ACCAGCTTTG AGCATGTCTA TCATATGACT 
GCCCACTCGG CTGAAAACTT TCTTTCCCAA GACTGCGCCC CACTTTCCAC CTACACCCCA 
TCACTGCGCT CTTTCCCCGG GACACCTGTC TCCATCTGCT CTCGGAGTGT CCGTCCCCAG 
GAGAGTCGCG CTGTCTTCAG TGGCTCTGTG AGCATCCCCT CCATCGCCAA GTCCCGGGGC 
GAGCCGGGCC GCTCCATGAG TGCCAGCAGT GGTCTGGGCA CACGGTCCAC AGCACAGAAC 
GGCAGTGCCC TGAGGAGGGA GTTCTCTGGT GGGAGCTATG GCTCCAAGCG CCAGGCCATG 
ACCTCTCCCT CTGAGGGATC CTTGTCCTCG GGTGGGGGGA CGGATGGTAC CACAGATGCT 
CCCATTTCAC AGTTCGACAG AGAGTGGCAG GCTTCAGAGA AGACCCCTGA TCTCAAAAGG 
GCATTAAGGA CCCTGCTTAG TAAGATGAAG GACTCAGACT CCCCCCGCCA CTCCACAGCA 
TCCAACAGCT CGAACCTGAG CAGCCCCCCA AGCCCAGCTT CCCCACACAA GACCAAGTCT 
CTGTCCCTGG AGAGCACAGA CCGCATGGGC TGGGAGACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42bpa ( XM_018735190.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018590706.1 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X1, mRNA.

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CloneID	OSc41989
Clone ID Related Accession (Same CDS sequence)	XM_018735190.1
Accession Version	XM_018735190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TTTGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGGACTCAGA CTCCCCCCGC CACTCCACAG CATCCAACAG CTCGAACCTG 
AGCAGCCCCC CAAGCCCAGC TTCCCCACAC AAGACCAAGT CTCTGTCCCT GGAGAGCACA 
GACCGCATGG GCTGGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018590706.1
CDS	538..5841
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDRSPSCVAPSKGRRIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREDSDSPRHSTASNSSNLSSPPSPASPHKTKSLSLESTDRMGWET

Target ORF information:

RefSeq Version	XM_018735190.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018735190.1

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TTTGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGAGTGTCC GTCCCCAGGA GAGTCGCGCT 
GTCTTCAGTG GCTCTGTGAG CATCCCCTCC ATCGCCAAGT CCCGGGGCGA GCCGGGCCGC 
TCCATGAGTG CCAGCAGTGG TCTGGGCACA CGGTCCACAG CACAGAACGG CAGTGCCCTG 
AGGAGGGAGT TCTCTGGTGG GAGCTATGGC TCCAAGCGCC AGGCCATGAC CTCTCCCTCT 
GAGGGATCCT TGTCCTCGGG TGGGGGGACG GATGGTACCA CAGATGCTCC CATTTCACAG 
TTCGACAGAG AGGACTCAGA CTCCCCCCGC CACTCCACAG CATCCAACAG CTCGAACCTG 
AGCAGCCCCC CAAGCCCAGC TTCCCCACAC AAGACCAAGT CTCTGTCCCT GGAGAGCACA 
GACCGCATGG GCTGGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42bpa ( XM_018735191.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018590707.1 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X2, mRNA.

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CloneID	OSc41990
Clone ID Related Accession (Same CDS sequence)	XM_018735191.1
Accession Version	XM_018735191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TATTGATTCT ATGGACAACT TCACTCCTTC CCGGGAAGAT 
GAGGCCAAAT CCCGCCTGAT GTCCCGATCA CGGTCGCCAT CCACAGCCAG TGACATGGAG 
CCACCGGAGA TAATAGACCA CCATCAACGG TCTGTTCTGA CCCCCACCGT GAAACTAGGC 
TTCAGTGGGT CTAGTCTAAC AGCCAAGCCC AAAGCCCACC AGTTTGTCGT GAAGTCCTTC 
AACACTCCCA CCAAGTGCAA CCAGTGCACC TCCCTCATGG TGGGACTCAT TCGCCAGGGC 
TGCACCTGTG AAGTCTGCAA CTTCTCATGT CATGTGACGT GTGCAGACAA GGCGCCAGCT 
GTTTGCCCAA TCCCACCAGA CCAGACCAAA GGTCCACTGG GCATCGACCC CCAGAAGGGC 
ATCGGCACAG CCTATGAAGG CCACGTTCGA GTGCCCAAGC CAGCAGGGGT GAAGAAAGGC 
TGGCAGCGGG CTCTTGCCGT TGTGTGTGAC TTCAAGCTGT TTCTGTATGA CCTGGCTGAA 
GGCAAGGCAT CCACCCCCAG CGTTGTGATC AGCCAGGTCA TTGACATGAG GGACGAGGAC 
TTCTCTGTCA GCTCAGTCTT GGCCTCCGAT GTTATTCATG CCAACCGGAA GGACATCCCC 
TGCATTTTCA GGGTGACAGC CTCTCAGCTG TCTGCCTCCA GCAGTCACAA GTGCTCCATC 
CTAATTCTGG CCGACAGCGA GCAGGAACGT AGCAAGTGGG TGGGGCTGCT GAACGAGATG 
CACCGTCTGC TGAGGAAGAG CAAGTTGAAG GACCGCTTTG TCTATGTGCC CAAAGAGGCC 
TATGACAGCA CGCTGCCTCT CATCAAGAGT GCGCAGTCTG CTGCCATCAT AGATCATGAG 
CGTGTTGCTC TTGGCAACGA AGAGGGTCTG TATGTCATCC ATGTCACCAA AGATGAAATT 
GTCCGGGTGG GCGACAATAA GAAGGTGCAC CACATTGAAC TGATGCCTTC AGAGCAGCTG 
CTGGCAGTCA TCTCTGGCCG CAACCGTCAC GTTCGTCTGT TCCCCATGTT GGCACTAGAT 
GGTCGTGAGA CGGACTCTTA CAAACTGGCA GAGACCAAAG GCTGCCAGAC ATTGGTGTCA 
GGCCGACCAC GGCCCGGTGG ACCCACTTGC CTTTGCGTGG CCATGAAACG GCAGGTGATT 
TGCTACGAGA TTAACAAAAG CAAGACACAT CACCGTAAGT TACGGGACTT CCAGGTGCCA 
GGTCCTGTAC AGTGGATGGC TCTCCTAGGC GACCGGCTGT GCGTAGGCTA CCAGGCTGGT 
TTTGCCCGCT ACAGCCCGCA TTCGGATGCA GTGCCTGTCA GCCTGCTCCA CCCCGAGGAC 
CCCACCCTGT CCTTCATTAG CCAGCAGGGC CTGGATGCCA TCTGTGCTGT GGAGATCTCT 
TCCAAGGAGT TCCTGCTGTG CTTCAGCTGC GTGGGTGTGT ACGTAGACTG CCAGGGCAGG 
AGGTCGCGTC AGCAGGAGCT CATGTGGCCA GCCGTGCCCA CCGCCTGCTG CTACAACGCC 
CCCTACCTGT CAGTGTACAG TGAGAATGCT GTGGATGTGT TTGACGTCAA CACCATGGAG 
TGGATCCAGA CCATTCCTTT GAAGAAGGTG CGGCCTCTCA ACACAGACGG CTCCCTCAAC 
CTGCTCGGCC TGGAGACAGT TCGGCTCATT TATTTCAAGA ACAAGATGGC AGAAGGAGAC 
GAGCTTGTAG TTCCTGAGAC ATCGGACAAC AGCCGGAAGC AGATGGTGCG CAGCATCAAC 
AATAAGAGAC GCTTTTCCTT CCGTGTACCT GAGGAAGAGA GGATGCAGCA AAGGAGAGAG 
ATGCTGAAGG ACCCAGAAAT GAGGAACAAG CTCATCTCCA ACCCCACCAA CTTTAACCAT 
GTTGTTCACA TGGGCCCAGG TGACGGCATC CAGATTCTCA AAGACCTTCC CATGAGTGTC 
CGTCCCCAGG AGAGTCGCGC TGTCTTCAGT GGCTCTGTGA GCATCCCCTC CATCGCCAAG 
TCCCGGGGCG AGCCGGGCCG CTCCATGAGT GCCAGCAGTG GTCTGGGCAC ACGGTCCACA 
GCACAGAACG GCAGTGCCCT GAGGAGGGAG TTCTCTGGTG GGAGCTATGG CTCCAAGCGC 
CAGGCCATGA CCTCTCCCTC TGAGGGATCC TTGTCCTCGG GTGGGGGGAC GGATGGTACC 
ACAGATGCTC CCATTTCACA GTTCGACAGA GAGGACTCAG ACTCCCCCCG CCACTCCACA 
GCATCCAACA GCTCGAACCT GAGCAGCCCC CCAAGCCCAG CTTCCCCACA CAAGACCAAG 
TCTCTGTCCC TGGAGAGCAC AGACCGCATG GGCTGGGAGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018590707.1
CDS	832..6096
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREDSDSPRHSTASNSSNLSSPPSPASPHKTKSLSLESTDRMGWET

Target ORF information:

RefSeq Version	XM_018735191.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018735191.1

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TATTGATTCT ATGGACAACT TCACTCCTTC CCGGGAAGAT 
GAGGCCAAAT CCCGCCTGAT GTCCCGATCA CGGTCGCCAT CCACAGCCAG TGACATGGAG 
CCACCGGAGA TAATAGACCA CCATCAACGG TCTGTTCTGA CCCCCACCGT GAAACTAGGC 
TTCAGTGGGT CTAGTCTAAC AGCCAAGCCC AAAGCCCACC AGTTTGTCGT GAAGTCCTTC 
AACACTCCCA CCAAGTGCAA CCAGTGCACC TCCCTCATGG TGGGACTCAT TCGCCAGGGC 
TGCACCTGTG AAGTCTGCAA CTTCTCATGT CATGTGACGT GTGCAGACAA GGCGCCAGCT 
GTTTGCCCAA TCCCACCAGA CCAGACCAAA GGTCCACTGG GCATCGACCC CCAGAAGGGC 
ATCGGCACAG CCTATGAAGG CCACGTTCGA GTGCCCAAGC CAGCAGGGGT GAAGAAAGGC 
TGGCAGCGGG CTCTTGCCGT TGTGTGTGAC TTCAAGCTGT TTCTGTATGA CCTGGCTGAA 
GGCAAGGCAT CCACCCCCAG CGTTGTGATC AGCCAGGTCA TTGACATGAG GGACGAGGAC 
TTCTCTGTCA GCTCAGTCTT GGCCTCCGAT GTTATTCATG CCAACCGGAA GGACATCCCC 
TGCATTTTCA GGGTGACAGC CTCTCAGCTG TCTGCCTCCA GCAGTCACAA GTGCTCCATC 
CTAATTCTGG CCGACAGCGA GCAGGAACGT AGCAAGTGGG TGGGGCTGCT GAACGAGATG 
CACCGTCTGC TGAGGAAGAG CAAGTTGAAG GACCGCTTTG TCTATGTGCC CAAAGAGGCC 
TATGACAGCA CGCTGCCTCT CATCAAGAGT GCGCAGTCTG CTGCCATCAT AGATCATGAG 
CGTGTTGCTC TTGGCAACGA AGAGGGTCTG TATGTCATCC ATGTCACCAA AGATGAAATT 
GTCCGGGTGG GCGACAATAA GAAGGTGCAC CACATTGAAC TGATGCCTTC AGAGCAGCTG 
CTGGCAGTCA TCTCTGGCCG CAACCGTCAC GTTCGTCTGT TCCCCATGTT GGCACTAGAT 
GGTCGTGAGA CGGACTCTTA CAAACTGGCA GAGACCAAAG GCTGCCAGAC ATTGGTGTCA 
GGCCGACCAC GGCCCGGTGG ACCCACTTGC CTTTGCGTGG CCATGAAACG GCAGGTGATT 
TGCTACGAGA TTAACAAAAG CAAGACACAT CACCGTAAGT TACGGGACTT CCAGGTGCCA 
GGTCCTGTAC AGTGGATGGC TCTCCTAGGC GACCGGCTGT GCGTAGGCTA CCAGGCTGGT 
TTTGCCCGCT ACAGCCCGCA TTCGGATGCA GTGCCTGTCA GCCTGCTCCA CCCCGAGGAC 
CCCACCCTGT CCTTCATTAG CCAGCAGGGC CTGGATGCCA TCTGTGCTGT GGAGATCTCT 
TCCAAGGAGT TCCTGCTGTG CTTCAGCTGC GTGGGTGTGT ACGTAGACTG CCAGGGCAGG 
AGGTCGCGTC AGCAGGAGCT CATGTGGCCA GCCGTGCCCA CCGCCTGCTG CTACAACGCC 
CCCTACCTGT CAGTGTACAG TGAGAATGCT GTGGATGTGT TTGACGTCAA CACCATGGAG 
TGGATCCAGA CCATTCCTTT GAAGAAGGTG CGGCCTCTCA ACACAGACGG CTCCCTCAAC 
CTGCTCGGCC TGGAGACAGT TCGGCTCATT TATTTCAAGA ACAAGATGGC AGAAGGAGAC 
GAGCTTGTAG TTCCTGAGAC ATCGGACAAC AGCCGGAAGC AGATGGTGCG CAGCATCAAC 
AATAAGAGAC GCTTTTCCTT CCGTGTACCT GAGGAAGAGA GGATGCAGCA AAGGAGAGAG 
ATGCTGAAGG ACCCAGAAAT GAGGAACAAG CTCATCTCCA ACCCCACCAA CTTTAACCAT 
GTTGTTCACA TGGGCCCAGG TGACGGCATC CAGATTCTCA AAGACCTTCC CATGAGTGTC 
CGTCCCCAGG AGAGTCGCGC TGTCTTCAGT GGCTCTGTGA GCATCCCCTC CATCGCCAAG 
TCCCGGGGCG AGCCGGGCCG CTCCATGAGT GCCAGCAGTG GTCTGGGCAC ACGGTCCACA 
GCACAGAACG GCAGTGCCCT GAGGAGGGAG TTCTCTGGTG GGAGCTATGG CTCCAAGCGC 
CAGGCCATGA CCTCTCCCTC TGAGGGATCC TTGTCCTCGG GTGGGGGGAC GGATGGTACC 
ACAGATGCTC CCATTTCACA GTTCGACAGA GAGGACTCAG ACTCCCCCCG CCACTCCACA 
GCATCCAACA GCTCGAACCT GAGCAGCCCC CCAAGCCCAG CTTCCCCACA CAAGACCAAG 
TCTCTGTCCC TGGAGAGCAC AGACCGCATG GGCTGGGAGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc42bpa ( XM_018735192.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018590708.1 Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X3, mRNA.

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CloneID	OSc41991
Clone ID Related Accession (Same CDS sequence)	XM_018735192.1
Accession Version	XM_018735192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TTTGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGCCTGGTT CACCCCTGCC CTCGTGCCCC 
TCCCCACACA CACGCCTCAT CTCCACTCCC ACCAGCTTTG AGCATGTCTA TCATATGACT 
GCCCACTCGG CTGAAAACTT TCTTTCCCAA GACTGCGCCC CACTTTCCAC CTACACCCCA 
TCACTGCGCT CTTTCCCCGG GACACCTGTC TCCATCTGCT CTCGGAGTGT CCGTCCCCAG 
GAGAGTCGCG CTGTCTTCAG TGGCTCTGTG AGCATCCCCT CCATCGCCAA GTCCCGGGGC 
GAGCCGGGCC GCTCCATGAG TGCCAGCAGT GGTCTGGGCA CACGGTCCAC AGCACAGAAC 
GGCAGTGCCC TGAGGAGGGA GTTCTCTGGT GGGAGCTATG GCTCCAAGCG CCAGGCCATG 
ACCTCTCCCT CTGAGGGATC CTTGTCCTCG GGTGGGGGGA CGGATGGTAC CACAGATGCT 
CCCATTTCAC AGTTCGACAG AGAGTGGCAG GCTTCAGAGA AGACCCCTGA TCTCAAAAGG 
GCATTAAGGA CCCTGCTTAG TAAGATGAAG GTAATATTGA CATGGCTGTG TGCGCTCACA 
CACCTGAGCT CGTGTTCCAG CAGCTGTGCC CCCCCCCCCA TCCCCTGCCA TGCCTCCACC 
CCCATCCCCT GCCATGCCTC ACTTTGGACA ACTGTTAGAG TCTCAGTTTC AGTTATAGTC 
AGTAAGCCCA CATGTACTGC AGTGGAACGG AGCCTCTTCA AAGGCGTGTT CTTCTGTCTC 
CTCCTTGCTG GAGGTGTTCA GAGAGCACAG TGGGGAGAGT TGCCCCCCAC CATGCACACA 
GTGCCTTGTT TAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018590708.1
CDS	832..6549
Translation	MMSGEVRLKKLEKLLLDGPAQSNGQCFSVETLLDVLVCLHDECNSSPLRREKNVLEFLEWAKPFTSKVKQMRLHKEDFEILKVIGRGAFGEVAVVKVKNTDKVFAMKILNKWEMLKRAETACFREERDVLVNGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVLAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLRLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPSHVTDVSENAKDLIRRLICSREHRLGQSGIEDFKQHPFFVGIDWDNIRSCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPSHTAFSGHHLPFVGFTYTSSCTLSDRGCLRESAGPAQMDVRLQRSLEESLATEAYERRIRRLEQEKLELSRKLQESTQTVQALQYPSTDGPVPASKEVEVRSLKEEIEKLKKQIAESGQLEQQLEQASIAQRDLEDSSKLVKSLEKQLKSTKQEKDDMQKEVLEANEKLKAQTKELKEAQSQKKAAMQEFSEMNQRVTELRSHKQRLTRQLRDKEEEMEGVSQKVEALRLEVRKVERAKKELEVQAEERAAEAQKERKLRERSEQYSRQLEDELEGLKQKQAGRSLGMGTSEQHQELSKLRAELEKKAVFYEEELSRRELLHAGELKGLRKELRDAEAQQLALRKEILVLKDKLEKTRRESQSEREEFETEYKQKYEREKILLSEENRKLSCELEKVTSMFEKLSSTNKQLEEEMRDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALATKMTEELEGLRSSSLGARPTDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQDELNKVKASSISTECKLQESESKNQDLLSEIERLKKEVEDLRFGKGVKHQDSQNSFLAFLNAPTSALDQFDRSPSCVAPSKGRRIDSMDNFTPSREDEAKSRLMSRSRSPSTASDMEPPEIIDHHQRSVLTPTVKLGFSGSSLTAKPKAHQFVVKSFNTPTKCNQCTSLMVGLIRQGCTCEVCNFSCHVTCADKAPAVCPIPPDQTKGPLGIDPQKGIGTAYEGHVRVPKPAGVKKGWQRALAVVCDFKLFLYDLAEGKASTPSVVISQVIDMRDEDFSVSSVLASDVIHANRKDIPCIFRVTASQLSASSSHKCSILILADSEQERSKWVGLLNEMHRLLRKSKLKDRFVYVPKEAYDSTLPLIKSAQSAAIIDHERVALGNEEGLYVIHVTKDEIVRVGDNKKVHHIELMPSEQLLAVISGRNRHVRLFPMLALDGRETDSYKLAETKGCQTLVSGRPRPGGPTCLCVAMKRQVICYEINKSKTHHRKLRDFQVPGPVQWMALLGDRLCVGYQAGFARYSPHSDAVPVSLLHPEDPTLSFISQQGLDAICAVEISSKEFLLCFSCVGVYVDCQGRRSRQQELMWPAVPTACCYNAPYLSVYSENAVDVFDVNTMEWIQTIPLKKVRPLNTDGSLNLLGLETVRLIYFKNKMAEGDELVVPETSDNSRKQMVRSINNKRRFSFRVPEEERMQQRREMLKDPEMRNKLISNPTNFNHVVHMGPGDGIQILKDLPMPGSPLPSCPSPHTRLISTPTSFEHVYHMTAHSAENFLSQDCAPLSTYTPSLRSFPGTPVSICSRSVRPQESRAVFSGSVSIPSIAKSRGEPGRSMSASSGLGTRSTAQNGSALRREFSGGSYGSKRQAMTSPSEGSLSSGGGTDGTTDAPISQFDREWQASEKTPDLKRALRTLLSKMKVILTWLCALTHLSSCSSSCAPPPIPCHASTPIPCHASLWTTVRVSVSVIVSKPTCTAVERSLFKGVFFCLLLAGGVQRAQWGELPPTMHTVPCLA

Target ORF information:

RefSeq Version	XM_018735192.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus CDC42 binding protein kinase alpha (cdc42bpa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018735192.1

ORF Insert Sequence:

ATGATGTCGG GCGAGGTGCG TCTGAAGAAG CTCGAGAAGC TGCTGCTGGA CGGACCGGCT 
CAGTCCAACG GGCAGTGCTT CAGCGTGGAG ACGCTGCTCG ACGTGCTCGT GTGTCTCCAC 
GACGAGTGCA ACAGCTCGCC GCTGCGCAGG GAGAAGAATG TGCTCGAGTT CCTCGAATGG 
GCCAAGCCCT TCACGTCCAA AGTGAAGCAG ATGCGCCTTC ATAAGGAAGA TTTTGAGATC 
CTGAAGGTCA TTGGCAGAGG AGCATTTGGA GAGGTGGCTG TGGTGAAGGT GAAGAATACA 
GACAAGGTCT TTGCCATGAA GATCCTCAAC AAATGGGAGA TGCTGAAACG GGCTGAGACG 
GCATGTTTTC GGGAGGAACG GGATGTGCTG GTGAACGGGG ACAGCCAGTG GATCACTACT 
TTGCACTATG CCTTTCAGGA TGAGAACTAC CTGTACCTGG TGATGGATTA CTATGTTGGA 
GGGGACCTGC TTACACTGCT GAGCAAGTTT GAGGACCGCC TTCCGGAGGA CATGGCCCGC 
TTCTATCTGG CTGAGATGGT GCTGGCCATC GACTCAGTGC ATCAGTTGCA CTACGTCCAC 
CGGGACATCA AGCCGGACAA CATCCTCATG GACATGAATG GACACATCAG ACTCGCTGAC 
TTTGGCTCCT GCCTAAGACT CATGGAGGAC GGAACGGTTC AGTCCTCTGT GGCAGTGGGC 
ACACCAGACT ACATTTCCCC TGAGATCCTC CAGGCCATGG AGGACGGGAA AGGAAAGTAT 
GGTCCGGAGT GTGACTGGTG GTCGCTAGGT GTCTGCATGT ACGAGATGTT GTATGGCGAG 
ACTCCTTTCT ACGCAGAGTC CTTGGTGGAG ACCTATGGCA AGATCATGAA CCACAAGGAA 
CGCTTTCAGT TCCCTTCCCA TGTGACCGAC GTCTCAGAGA ACGCGAAAGA CCTGATCCGC 
CGCCTGATCT GCTCTCGGGA GCATCGGCTC GGTCAGAGTG GTATCGAGGA CTTCAAGCAA 
CATCCTTTCT TTGTGGGCAT CGACTGGGAC AACATCCGCA GTTGTGAAGC ACCCTACATC 
CCTGAAGTCA GCAGCCCCAC AGACACGTCC AACTTTGATG TGGATGACGA CTGCCTTAAG 
AACTCTGAAA CCATGCCACC TCCCTCTCAC ACTGCCTTCT CTGGACACCA CTTACCCTTT 
GTGGGATTCA CCTACACAAG CAGTTGCACA TTATCGGATC GAGGCTGTTT GCGCGAGTCA 
GCAGGGCCTG CTCAGATGGA TGTGCGGCTA CAGCGAAGCC TGGAAGAGAG TCTAGCGACG 
GAGGCATACG AGCGCCGAAT ACGGCGGCTG GAACAAGAAA AGTTGGAACT AAGTCGAAAG 
CTGCAAGAGT CCACACAGAC CGTGCAGGCG CTCCAGTACC CCAGTACAGA CGGACCTGTT 
CCAGCCAGTA AAGAGGTGGA AGTTCGAAGC CTTAAGGAGG AAATTGAGAA GCTAAAGAAG 
CAGATAGCAG AGTCTGGTCA GCTGGAGCAG CAGCTGGAGC AAGCCAGCAT CGCACAAAGG 
GACCTGGAGG ACTCCTCCAA GCTCGTCAAG TCTCTGGAGA AGCAATTGAA AAGCACGAAG 
CAGGAGAAAG ATGACATGCA GAAGGAGGTG CTGGAGGCCA ATGAGAAACT CAAGGCCCAG 
ACTAAGGAGC TGAAGGAGGC ACAGAGCCAG AAGAAAGCGG CCATGCAGGA GTTTTCTGAG 
ATGAACCAGC GTGTGACGGA GCTGCGCTCG CACAAGCAAC GCCTCACCCG TCAACTTCGT 
GACAAAGAGG AGGAGATGGA AGGGGTCTCG CAGAAAGTTG AGGCACTACG CTTGGAGGTG 
CGCAAGGTGG AGAGAGCGAA GAAGGAGCTG GAGGTACAGG CAGAAGAACG TGCAGCAGAG 
GCGCAGAAGG AGCGGAAGTT GAGGGAGCGT AGTGAACAAT ATAGCAGGCA GCTCGAGGAT 
GAGTTGGAGG GACTCAAGCA GAAGCAGGCA GGTCGTTCAC TGGGCATGGG GACTTCTGAG 
CAGCACCAGG AGCTAAGCAA GCTGAGGGCA GAGCTGGAGA AGAAGGCGGT GTTCTATGAA 
GAGGAACTGT CACGGAGGGA GCTGCTGCAT GCCGGCGAGC TCAAAGGACT AAGGAAGGAG 
TTGCGTGATG CTGAGGCCCA GCAACTGGCG CTCCGCAAGG AGATCCTGGT GCTCAAGGAC 
AAGCTGGAGA AGACACGCCG TGAGAGTCAG AGCGAGCGGG AGGAGTTTGA AACGGAATAC 
AAGCAGAAAT ATGAAAGGGA GAAGATCCTG CTGTCAGAGG AAAACAGGAA GCTCTCCTGT 
GAGCTGGAGA AGGTGACATC TATGTTTGAA AAGCTGAGCA GCACCAACAA GCAGCTGGAG 
GAGGAGATGA GGGACCTGGC AGACAAAAAG GAGAGTGTAG CTCATTGGGA GGCCCAGATC 
ACAGAGATCA TCCAGTGGGT GAGTGATGAG AAGGACGCCC GTGGCTACCT GCAGGCCCTG 
GCCACCAAGA TGACTGAGGA GCTGGAGGGT CTTAGGAGCT CAAGCCTGGG TGCACGCCCC 
ACGGACATGC CCTGGAAGAT GCGGCGCTTC GCCAAGCTGG ACATGTCTGC CCGGTTGGAG 
CTGCAGTCTG CATTGGATGC TGAGATCCGT GCCAAGCAGG CCATCCAGGA TGAGCTCAAC 
AAGGTCAAGG CTTCCAGCAT CTCCACAGAA TGTAAACTGC AGGAGTCTGA GAGCAAGAAC 
CAGGACCTGC TGTCTGAGAT TGAGAGACTG AAGAAAGAGG TGGAAGATCT GCGCTTTGGG 
AAGGGTGTGA AACATCAGGA CTCCCAGAAC TCATTTCTTG CATTCTTGAA TGCCCCCACA 
TCTGCCTTGG ATCAGTTTGA TCGTTCCCCT TCCTGTGTCG CACCTAGCAA AGGCAGACGT 
ATTGATTCTA TGGACAACTT CACTCCTTCC CGGGAAGATG AGGCCAAATC CCGCCTGATG 
TCCCGATCAC GGTCGCCATC CACAGCCAGT GACATGGAGC CACCGGAGAT AATAGACCAC 
CATCAACGGT CTGTTCTGAC CCCCACCGTG AAACTAGGCT TCAGTGGGTC TAGTCTAACA 
GCCAAGCCCA AAGCCCACCA GTTTGTCGTG AAGTCCTTCA ACACTCCCAC CAAGTGCAAC 
CAGTGCACCT CCCTCATGGT GGGACTCATT CGCCAGGGCT GCACCTGTGA AGTCTGCAAC 
TTCTCATGTC ATGTGACGTG TGCAGACAAG GCGCCAGCTG TTTGCCCAAT CCCACCAGAC 
CAGACCAAAG GTCCACTGGG CATCGACCCC CAGAAGGGCA TCGGCACAGC CTATGAAGGC 
CACGTTCGAG TGCCCAAGCC AGCAGGGGTG AAGAAAGGCT GGCAGCGGGC TCTTGCCGTT 
GTGTGTGACT TCAAGCTGTT TCTGTATGAC CTGGCTGAAG GCAAGGCATC CACCCCCAGC 
GTTGTGATCA GCCAGGTCAT TGACATGAGG GACGAGGACT TCTCTGTCAG CTCAGTCTTG 
GCCTCCGATG TTATTCATGC CAACCGGAAG GACATCCCCT GCATTTTCAG GGTGACAGCC 
TCTCAGCTGT CTGCCTCCAG CAGTCACAAG TGCTCCATCC TAATTCTGGC CGACAGCGAG 
CAGGAACGTA GCAAGTGGGT GGGGCTGCTG AACGAGATGC ACCGTCTGCT GAGGAAGAGC 
AAGTTGAAGG ACCGCTTTGT CTATGTGCCC AAAGAGGCCT ATGACAGCAC GCTGCCTCTC 
ATCAAGAGTG CGCAGTCTGC TGCCATCATA GATCATGAGC GTGTTGCTCT TGGCAACGAA 
GAGGGTCTGT ATGTCATCCA TGTCACCAAA GATGAAATTG TCCGGGTGGG CGACAATAAG 
AAGGTGCACC ACATTGAACT GATGCCTTCA GAGCAGCTGC TGGCAGTCAT CTCTGGCCGC 
AACCGTCACG TTCGTCTGTT CCCCATGTTG GCACTAGATG GTCGTGAGAC GGACTCTTAC 
AAACTGGCAG AGACCAAAGG CTGCCAGACA TTGGTGTCAG GCCGACCACG GCCCGGTGGA 
CCCACTTGCC TTTGCGTGGC CATGAAACGG CAGGTGATTT GCTACGAGAT TAACAAAAGC 
AAGACACATC ACCGTAAGTT ACGGGACTTC CAGGTGCCAG GTCCTGTACA GTGGATGGCT 
CTCCTAGGCG ACCGGCTGTG CGTAGGCTAC CAGGCTGGTT TTGCCCGCTA CAGCCCGCAT 
TCGGATGCAG TGCCTGTCAG CCTGCTCCAC CCCGAGGACC CCACCCTGTC CTTCATTAGC 
CAGCAGGGCC TGGATGCCAT CTGTGCTGTG GAGATCTCTT CCAAGGAGTT CCTGCTGTGC 
TTCAGCTGCG TGGGTGTGTA CGTAGACTGC CAGGGCAGGA GGTCGCGTCA GCAGGAGCTC 
ATGTGGCCAG CCGTGCCCAC CGCCTGCTGC TACAACGCCC CCTACCTGTC AGTGTACAGT 
GAGAATGCTG TGGATGTGTT TGACGTCAAC ACCATGGAGT GGATCCAGAC CATTCCTTTG 
AAGAAGGTGC GGCCTCTCAA CACAGACGGC TCCCTCAACC TGCTCGGCCT GGAGACAGTT 
CGGCTCATTT ATTTCAAGAA CAAGATGGCA GAAGGAGACG AGCTTGTAGT TCCTGAGACA 
TCGGACAACA GCCGGAAGCA GATGGTGCGC AGCATCAACA ATAAGAGACG CTTTTCCTTC 
CGTGTACCTG AGGAAGAGAG GATGCAGCAA AGGAGAGAGA TGCTGAAGGA CCCAGAAATG 
AGGAACAAGC TCATCTCCAA CCCCACCAAC TTTAACCATG TTGTTCACAT GGGCCCAGGT 
GACGGCATCC AGATTCTCAA AGACCTTCCC ATGCCTGGTT CACCCCTGCC CTCGTGCCCC 
TCCCCACACA CACGCCTCAT CTCCACTCCC ACCAGCTTTG AGCATGTCTA TCATATGACT 
GCCCACTCGG CTGAAAACTT TCTTTCCCAA GACTGCGCCC CACTTTCCAC CTACACCCCA 
TCACTGCGCT CTTTCCCCGG GACACCTGTC TCCATCTGCT CTCGGAGTGT CCGTCCCCAG 
GAGAGTCGCG CTGTCTTCAG TGGCTCTGTG AGCATCCCCT CCATCGCCAA GTCCCGGGGC 
GAGCCGGGCC GCTCCATGAG TGCCAGCAGT GGTCTGGGCA CACGGTCCAC AGCACAGAAC 
GGCAGTGCCC TGAGGAGGGA GTTCTCTGGT GGGAGCTATG GCTCCAAGCG CCAGGCCATG 
ACCTCTCCCT CTGAGGGATC CTTGTCCTCG GGTGGGGGGA CGGATGGTAC CACAGATGCT 
CCCATTTCAC AGTTCGACAG AGAGTGGCAG GCTTCAGAGA AGACCCCTGA TCTCAAAAGG 
GCATTAAGGA CCCTGCTTAG TAAGATGAAG GTAATATTGA CATGGCTGTG TGCGCTCACA 
CACCTGAGCT CGTGTTCCAG CAGCTGTGCC CCCCCCCCCA TCCCCTGCCA TGCCTCCACC 
CCCATCCCCT GCCATGCCTC ACTTTGGACA ACTGTTAGAG TCTCAGTTTC AGTTATAGTC 
AGTAAGCCCA CATGTACTGC AGTGGAACGG AGCCTCTTCA AAGGCGTGTT CTTCTGTCTC 
CTCCTTGCTG GAGGTGTTCA GAGAGCACAG TGGGGAGAGT TGCCCCCCAC CATGCACACA 
GTGCCTTGTT TAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

cdc42bpa cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム