ecm29 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ecm29
Entrez Gene ID	3361455
Full Name	proteasome component Ecm29
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001713058.2	XP_001713110.1	proteasome component Ecm29
NM_001356178.1	NP_001342940.1	proteasome component Ecm29

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ecm29 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ecm29 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102082	NM_001356178.1 Latest version!	Schizosaccharomyces pombe proteasome component Ecm29 (ecm29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSj04908	XM_001713058.2 Latest version!	Schizosaccharomyces pombe 972h- proteasome component Ecm29 (ecm29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ecm29 ( NM_001356178.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342940.1 Schizosaccharomyces pombe proteasome component Ecm29 (ecm29), mRNA.

Price & Availability↑

CloneID	OSc102082
Clone ID Related Accession (Same CDS sequence)	NM_001356178.1
Accession Version	NM_001356178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	proteasome component Ecm29
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGGCTGAAA ATGAATTGAG ATTATTAAAC AATGCAGAGC TGAAGCTCGC TCTAGCGGAA 
AGCGAGGACT CCTTTCAATC GCTTGTGTCA GTTTTTTTAT GTCCGATTTT ACTAAAACTC 
GATTCGCCTC ATGAAAGTGT GAGAAACAAA ACAATTTCAA TTGCAAACCA CATTATGACT 
AGGCTCAACA ATAACGCGCA AGCCATACTA CCTCTTGAAG CCTTAGTAAG CCAGTACGTT 
GAAGCAAATC AACCTTTGCG AAAAAGATTT CTCCTTGCTT TCATATCTAT AGGCGAAAAA 
AGGATACCTT GTTCTGAAAA TTTGGCCCTT CTCCAGATTT GTCTAAATCA TGTCAATGAA 
TACCCATTAG TATTATTGAC ATTGACAATT CGTTTACTTC GTTTTTCAAA GCCAACTTCG 
GCTATTACAT GCTCTAACGA TGTCATTTTA TCTCTTTCTT CATTCTATTT GATTCAGAAA 
GATCAAAGGA TTTTTAGTGA CCTGCAATTT TCCCAAAAAA CAGTAGATTA CCGCTTGAGT 
CTGCTTCGGT GGATTCATTT ATCTAGCTGG CCATCAAATT GGAAGTGGTT GGCTTACTTT 
TTTGCCTCTG CTGATTCTCA TTCTGAAGTT GCCCGACTGG CAGATGAATT TACGCGTGAT 
AGTGGATTAC CTGATCTAGA GAATTTGTCC CACGTGAACG TTCTATTAGA CATAGCGCTT 
GATAAGTTTC GAATCGAAGC TTTGCACGCA AACTTTTCTG TTTCCATTAG CTTAAGGAAC 
AAGGCAATTC AGCATTTACT TAAAAGTAAA ATTGCTGCGA ATACTGACAA AGCTATAAAT 
TGTATTGAAT TCATTCTTGA AGCACCTCCT AGTATGCAAC CCCGGTTAAT ACAGTTTACT 
CGTTGGGTAG TTGACAAAGC TGATCCAAAT TTTCTTAAAC CTAAAGCTGC GATGATATTG 
GAAAAAATTC TTAGCATCTT ATCGAGTAAT ATAATTCAGA GTGATTTACT TAGAGGATTT 
TTATATACAA CGATAGGTTT ACTGACCAAG GTTGATAATC ATCTGATTAC TAATTCTTTG 
CTGACAAATT TATTGACCTC CTTACAATCT GAATTACCAG ACGTACGAGT TTCAATTGAC 
GAAGCTCTTT CTATTATTAT TCCGTACTAT AGTAATTTTC GATTTTCTAA CGAATTGTTG 
CCAGTATTAG AACCCTTTAT TTTTGATTCA CCCGAGTCTC CTGCTGCGTA TTGCGCACTT 
CGATTTGTAT TGGTTGCTTT TCCTTTTGAT TATTTGCCAG CTCGTTTTAT TTGTCTAAAG 
GTACAAAATC CTTTCGTATT TCACCACTCT TTTATTGAAG AAGCTAAAAA AGGACTAAAC 
TTATCTCAAT GGGTTCAGTA TAATAGTGTG TATTCCACGA ATGAAGCTCA AGAGGAAGAT 
AAAGTACGTG CCGCTAGTTA TCCTTCTGCC TCTGAAGTAA TCTCTTTTAT TTTGTCGGAC 
CATGATTTAA AAAAATTTTG GGAATCTAAT GCTGCAGAGT ATTGTTTGGC CATTCTTGAG 
TTTATTGAGA GATGCATTTA TTATTCAGCG GATAGAAGCT TAGAGCTTTA CGACAATGAC 
AAGCTATCTT CTATAGACGC TTTACTGATT CAAGATTCCA AGTTAAGAGA AATGGTGTCT 
GAAAAGTGCA TTTCTTTGTC AAATTTTAAT GTATTTTTAG AATATGTTTT TTATGGTACT 
CTACTCATGC ACTTTGAACC CACTTACGCC CTCAGCAGAT TGGTATCCTT TGCACCACCT 
GAAGTTACAT TTTCTTTACC GGAGTTAGAT TTTCTAACGT CTGTATTTAA CTTTCCACTT 
GCTTTGCGTA ATACTGCAAC ACGAATACTG GGAATAATAC TTAGTACAAA AGATTCAACT 
CGTATATCAG AAGTACTTTC TTCTTGCTTC ACTATTATCT CAACGTCTAA CAATAAAAAT 
GACAATTTTT TCAAGGCTGA GACTGCTCTT CTAATCATCG GCTATACTAT CTCATACTTG 
GCTGCTCAAA CTAATTCAGC AGCCGTCGAT TCTTTCATTC TCAATTCTGG CAGTATAAAA 
GAATTTTTTT CTGTTTTATT GGAATATCTT GGTTCCAATG TTTTGCACAA AAAGACCACT 
TCACTTGCTA TTTATAAAGA ATTGTTTGTT TATTTTACTA GAGACTGGAT TACTTCGTAT 
GGCGTTGACT TTGACGAAAT TTTAAATGTT TTGCTACGCT TTCTAAAAGA GGTTGAAGAT 
ACCAACGTAA AGGTCGAATG TTTACACGTT ATATCACGTA TGTCGTTATC ATTTTCAGAT 
GATGAAATGG CAGAAAAGAT TCTTAAAGCC ATCTATGTTA CATATCACAT GGACTCTCCT 
GATATATTGT TTGCGAGTGC TGAAGCCATG TCGATACTTG CTGGTGGACA TAGAAACGTT 
TTCGTGAAAT CCACGTGCCC CATTTTTTTT CAAAAACAAT TGGACAATTA TAAAGCAGAT 
CACTATTGCT TTACCCTTGA TTTCATTTTA ACTGATTGTG TCAATTCTCC AAAACCCTTA 
CTTCGTAGGG CCTCTTCTTT ATGGCTTTTT TACATAGTTC GCTATTGCGA ACCTCAAACT 
TATACAATGA CGCGGCTTAA TGATATTTAT CATTCATTTC TCTCATTTTT GGTTACTCAA 
GATGATTTTG TTCAGGATAC TGCGTCAAGA GGCCTCAAAG CTATGTATGA TGTTTTAGAA 
GGGGACGAAA GAAAATCATT TACTGATAAT CTGATAAGCA CAATTGCTGC TGACCGCGTT 
GATGAAAAAA CCAAAGCTCC TTTGGACGCA GATACTGCCC TTTTCACTAC AAATAAAGGT 
ACTGTAGCCA CCTATAAGGA TATCTGTTCT TTAGCATCCG AGAGTGGTAA TCCTGATTTG 
ATTTATTCAT TCTTGAGCAT AGCAGGTAAC AGTTCTCTCT GGCAAGCCCG TAAGGGATTG 
GCATCTGGTA TAAGTTATTT AGGTATTCCC GAGGATCAAA AAAGAAAGAC GTTTTCATTT 
GATACTTCTA AAAGCTCTTC ATTATTAAAA AAGCTTTATA GATTTAAACA TGACCCTAAT 
CCTGATGTTG CCAAAACAAT GGGTGAAATT TGGGACACAT TAGTACCTTC AGATCTCAAT 
TTGGCATCTC ATCGTAAATA CTTAGTGGAG GATTGCCTCG AGTTTATGGG GAGCAGATCT 
TGGAGAGATA GGGAATCCAG TGTAAACACT TTGGTTAGTC TTCTTTCTAA TGTTCCTGTG 
ACCGAGTATC TAAATCAATT AGAGGATATA TGGAACATGT CTTTTCGAAC TCTTGATGAT 
ATTAAAGAGA GCGTTAGGGA AGCGTCCTTT CCACTTTGTA AGCTTTTAGC AAGATCAGTT 
ATTCAATCTT TGGAGAAGAC TTCACACAAT ACTAGCCCAA GCGGAATTTG CAAAGGCAAA 
CGGATTGTTT CAGTAGCTTT ACCATTTCTA TTAAAACACG CTTATGATCA GGCTAAAGAA 
GTTCGTTCAC TTACATACTC TACCATCACT GAACTTGTGC GTACTGGCAA TTCTACTTTG 
ACTTCGTTTG TGCCGGCCAT TATGCAGGTT ATGCTTGAAT ATTTGACAGA GTATGAATCA 
AAAGCTGCTA CCTTTTTGGA TTTTCACGCC AAAAATTACA GCATTAAACA AGAAAATATT 
GACAACGCCA GAACGTCAGC CGTTCAGAGC TCTTCAATGA TGGATACGCT AGAGAAGTGT 
ATCGGCCTTT TGGATGAAAG CTCAATGCAA ACATTATATC CAATATTAAA TAGAATGATT 
GCTAAACCTG GGGGTGTACC TACCAAAATA GGCAGCGCCC AAGTGGTGAT GCTTTTAGTA 
ATTCGTCGTG GACCACTCGT CAAGCAGTTT GCTTCAAAGC TGCTTCAATC CTTGAAATCT 
TCTTGCTTTG ATAGAAATGC TGCAGTAAGT GATGCATTTG CATCTGCTAT TGGCTATTTG 
TTACGAGTTT GCCCGTTGGA AATAGCTTCA CAAACTTGTC AAGAAATCAT CGATAAATTC 
TACGATGGAA ATACCAACGA ACAAATAATC AGTTCCAAAT TGACCGTGTA TGCGTCGAGA 
TACGCACCCG ACGTCTTCCT TAATCTTGGT TCTCTTTTTT TTCCTTTCAT TTTTTTTGGG 
AAACATTCTT CTTCCATTTC GATAAATGGG GTTTTAAGTA AAGCATGGGA TGAACTTTCA 
TCTGCCGGAA GCAGTGTGAA CCTGTATTCG GAAGAAATTA TCCTTCTTAT CCAAAAAAAC 
TTAATTGTTA CAAAATGGGA TGTCAAACGT CCTGCTGCTG CTGCATTATT GGAATTTGTC 
AATACTTCGC GACTCACTTA TCGACAAAAT GATATCTACG TGCTTTTAAA TGAAACAATG 
AAAGATAAAA GCTGGCCTGG GAAGGAATTA CTGCTTGAGG CTTATGTTAA ATTTTTAATT 
AAATATCCTG AGTTCATTAA ATCGCAAAAA ATGGAAGAAG TTCACCAAGT GATTGTTAGA 
GAATTTAAGA GACGCAATAT TGTTTACAAA AGCCATGCAA TGGAAAGTGT TGGTGAACTG 
TTATCTGATG AAAATTATAG AGAGCTTGAT TTGTATGAAC TATCATTAAA TGAATGTGGT 
ACATTTTTGC AGAAGGAATG GTTTGATAAA GATGATGAAT TGAATCTTGA GGAAAAAATT 
GCGCTTCAGC GCAATAGCGT CTATGCCATG TTTAATTCCA GCAGACCTGG AAATAAGAAT 
TGTAATGAGA TGTTGCTAAC TTACTTGTCA AATGCCCTTG ATGAAAATTA TTTACATTGG 
AATGTGAAAT TAGCAATTCT GAAAAATGCT CCTCACTTAA AAAAGATAAT GTCTAACGAG 
GAATTCTTAC TGTATAAAGA TATTCTTTAT CGTTGTTACG AAGACAATCC GTCACCAAAA 
GCAAAAGATT ATGCTGAAGT CATCTTCGGC GAAAATTATT TAAGTGTTTT AAGAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342940.1
CDS	146..5185
Translation	MAENELRLLNNAELKLALAESEDSFQSLVSVFLCPILLKLDSPHESVRNKTISIANHIMTRLNNNAQAILPLEALVSQYVEANQPLRKRFLLAFISIGEKRIPCSENLALLQICLNHVNEYPLVLLTLTIRLLRFSKPTSAITCSNDVILSLSSFYLIQKDQRIFSDLQFSQKTVDYRLSLLRWIHLSSWPSNWKWLAYFFASADSHSEVARLADEFTRDSGLPDLENLSHVNVLLDIALDKFRIEALHANFSVSISLRNKAIQHLLKSKIAANTDKAINCIEFILEAPPSMQPRLIQFTRWVVDKADPNFLKPKAAMILEKILSILSSNIIQSDLLRGFLYTTIGLLTKVDNHLITNSLLTNLLTSLQSELPDVRVSIDEALSIIIPYYSNFRFSNELLPVLEPFIFDSPESPAAYCALRFVLVAFPFDYLPARFICLKVQNPFVFHHSFIEEAKKGLNLSQWVQYNSVYSTNEAQEEDKVRAASYPSASEVISFILSDHDLKKFWESNAAEYCLAILEFIERCIYYSADRSLELYDNDKLSSIDALLIQDSKLREMVSEKCISLSNFNVFLEYVFYGTLLMHFEPTYALSRLVSFAPPEVTFSLPELDFLTSVFNFPLALRNTATRILGIILSTKDSTRISEVLSSCFTIISTSNNKNDNFFKAETALLIIGYTISYLAAQTNSAAVDSFILNSGSIKEFFSVLLEYLGSNVLHKKTTSLAIYKELFVYFTRDWITSYGVDFDEILNVLLRFLKEVEDTNVKVECLHVISRMSLSFSDDEMAEKILKAIYVTYHMDSPDILFASAEAMSILAGGHRNVFVKSTCPIFFQKQLDNYKADHYCFTLDFILTDCVNSPKPLLRRASSLWLFYIVRYCEPQTYTMTRLNDIYHSFLSFLVTQDDFVQDTASRGLKAMYDVLEGDERKSFTDNLISTIAADRVDEKTKAPLDADTALFTTNKGTVATYKDICSLASESGNPDLIYSFLSIAGNSSLWQARKGLASGISYLGIPEDQKRKTFSFDTSKSSSLLKKLYRFKHDPNPDVAKTMGEIWDTLVPSDLNLASHRKYLVEDCLEFMGSRSWRDRESSVNTLVSLLSNVPVTEYLNQLEDIWNMSFRTLDDIKESVREASFPLCKLLARSVIQSLEKTSHNTSPSGICKGKRIVSVALPFLLKHAYDQAKEVRSLTYSTITELVRTGNSTLTSFVPAIMQVMLEYLTEYESKAATFLDFHAKNYSIKQENIDNARTSAVQSSSMMDTLEKCIGLLDESSMQTLYPILNRMIAKPGGVPTKIGSAQVVMLLVIRRGPLVKQFASKLLQSLKSSCFDRNAAVSDAFASAIGYLLRVCPLEIASQTCQEIIDKFYDGNTNEQIISSKLTVYASRYAPDVFLNLGSLFFPFIFFGKHSSSISINGVLSKAWDELSSAGSSVNLYSEEIILLIQKNLIVTKWDVKRPAAAALLEFVNTSRLTYRQNDIYVLLNETMKDKSWPGKELLLEAYVKFLIKYPEFIKSQKMEEVHQVIVREFKRRNIVYKSHAMESVGELLSDENYRELDLYELSLNECGTFLQKEWFDKDDELNLEEKIALQRNSVYAMFNSSRPGNKNCNEMLLTYLSNALDENYLHWNVKLAILKNAPHLKKIMSNEEFLLYKDILYRCYEDNPSPKAKDYAEVIFGENYLSVLRN

Target ORF information:

RefSeq Version	NM_001356178.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe proteasome component Ecm29 (ecm29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356178.1

ORF Insert Sequence:

ATGGCTGAAA ATGAATTGAG ATTATTAAAC AATGCAGAGC TGAAGCTCGC TCTAGCGGAA 
AGCGAGGACT CCTTTCAATC GCTTGTGTCA GTTTTTTTAT GTCCGATTTT ACTAAAACTC 
GATTCGCCTC ATGAAAGTGT GAGAAACAAA ACAATTTCAA TTGCAAACCA CATTATGACT 
AGGCTCAACA ATAACGCGCA AGCCATACTA CCTCTTGAAG CCTTAGTAAG CCAGTACGTT 
GAAGCAAATC AACCTTTGCG AAAAAGATTT CTCCTTGCTT TCATATCTAT AGGCGAAAAA 
AGGATACCTT GTTCTGAAAA TTTGGCCCTT CTCCAGATTT GTCTAAATCA TGTCAATGAA 
TACCCATTAG TATTATTGAC ATTGACAATT CGTTTACTTC GTTTTTCAAA GCCAACTTCG 
GCTATTACAT GCTCTAACGA TGTCATTTTA TCTCTTTCTT CATTCTATTT GATTCAGAAA 
GATCAAAGGA TTTTTAGTGA CCTGCAATTT TCCCAAAAAA CAGTAGATTA CCGCTTGAGT 
CTGCTTCGGT GGATTCATTT ATCTAGCTGG CCATCAAATT GGAAGTGGTT GGCTTACTTT 
TTTGCCTCTG CTGATTCTCA TTCTGAAGTT GCCCGACTGG CAGATGAATT TACGCGTGAT 
AGTGGATTAC CTGATCTAGA GAATTTGTCC CACGTGAACG TTCTATTAGA CATAGCGCTT 
GATAAGTTTC GAATCGAAGC TTTGCACGCA AACTTTTCTG TTTCCATTAG CTTAAGGAAC 
AAGGCAATTC AGCATTTACT TAAAAGTAAA ATTGCTGCGA ATACTGACAA AGCTATAAAT 
TGTATTGAAT TCATTCTTGA AGCACCTCCT AGTATGCAAC CCCGGTTAAT ACAGTTTACT 
CGTTGGGTAG TTGACAAAGC TGATCCAAAT TTTCTTAAAC CTAAAGCTGC GATGATATTG 
GAAAAAATTC TTAGCATCTT ATCGAGTAAT ATAATTCAGA GTGATTTACT TAGAGGATTT 
TTATATACAA CGATAGGTTT ACTGACCAAG GTTGATAATC ATCTGATTAC TAATTCTTTG 
CTGACAAATT TATTGACCTC CTTACAATCT GAATTACCAG ACGTACGAGT TTCAATTGAC 
GAAGCTCTTT CTATTATTAT TCCGTACTAT AGTAATTTTC GATTTTCTAA CGAATTGTTG 
CCAGTATTAG AACCCTTTAT TTTTGATTCA CCCGAGTCTC CTGCTGCGTA TTGCGCACTT 
CGATTTGTAT TGGTTGCTTT TCCTTTTGAT TATTTGCCAG CTCGTTTTAT TTGTCTAAAG 
GTACAAAATC CTTTCGTATT TCACCACTCT TTTATTGAAG AAGCTAAAAA AGGACTAAAC 
TTATCTCAAT GGGTTCAGTA TAATAGTGTG TATTCCACGA ATGAAGCTCA AGAGGAAGAT 
AAAGTACGTG CCGCTAGTTA TCCTTCTGCC TCTGAAGTAA TCTCTTTTAT TTTGTCGGAC 
CATGATTTAA AAAAATTTTG GGAATCTAAT GCTGCAGAGT ATTGTTTGGC CATTCTTGAG 
TTTATTGAGA GATGCATTTA TTATTCAGCG GATAGAAGCT TAGAGCTTTA CGACAATGAC 
AAGCTATCTT CTATAGACGC TTTACTGATT CAAGATTCCA AGTTAAGAGA AATGGTGTCT 
GAAAAGTGCA TTTCTTTGTC AAATTTTAAT GTATTTTTAG AATATGTTTT TTATGGTACT 
CTACTCATGC ACTTTGAACC CACTTACGCC CTCAGCAGAT TGGTATCCTT TGCACCACCT 
GAAGTTACAT TTTCTTTACC GGAGTTAGAT TTTCTAACGT CTGTATTTAA CTTTCCACTT 
GCTTTGCGTA ATACTGCAAC ACGAATACTG GGAATAATAC TTAGTACAAA AGATTCAACT 
CGTATATCAG AAGTACTTTC TTCTTGCTTC ACTATTATCT CAACGTCTAA CAATAAAAAT 
GACAATTTTT TCAAGGCTGA GACTGCTCTT CTAATCATCG GCTATACTAT CTCATACTTG 
GCTGCTCAAA CTAATTCAGC AGCCGTCGAT TCTTTCATTC TCAATTCTGG CAGTATAAAA 
GAATTTTTTT CTGTTTTATT GGAATATCTT GGTTCCAATG TTTTGCACAA AAAGACCACT 
TCACTTGCTA TTTATAAAGA ATTGTTTGTT TATTTTACTA GAGACTGGAT TACTTCGTAT 
GGCGTTGACT TTGACGAAAT TTTAAATGTT TTGCTACGCT TTCTAAAAGA GGTTGAAGAT 
ACCAACGTAA AGGTCGAATG TTTACACGTT ATATCACGTA TGTCGTTATC ATTTTCAGAT 
GATGAAATGG CAGAAAAGAT TCTTAAAGCC ATCTATGTTA CATATCACAT GGACTCTCCT 
GATATATTGT TTGCGAGTGC TGAAGCCATG TCGATACTTG CTGGTGGACA TAGAAACGTT 
TTCGTGAAAT CCACGTGCCC CATTTTTTTT CAAAAACAAT TGGACAATTA TAAAGCAGAT 
CACTATTGCT TTACCCTTGA TTTCATTTTA ACTGATTGTG TCAATTCTCC AAAACCCTTA 
CTTCGTAGGG CCTCTTCTTT ATGGCTTTTT TACATAGTTC GCTATTGCGA ACCTCAAACT 
TATACAATGA CGCGGCTTAA TGATATTTAT CATTCATTTC TCTCATTTTT GGTTACTCAA 
GATGATTTTG TTCAGGATAC TGCGTCAAGA GGCCTCAAAG CTATGTATGA TGTTTTAGAA 
GGGGACGAAA GAAAATCATT TACTGATAAT CTGATAAGCA CAATTGCTGC TGACCGCGTT 
GATGAAAAAA CCAAAGCTCC TTTGGACGCA GATACTGCCC TTTTCACTAC AAATAAAGGT 
ACTGTAGCCA CCTATAAGGA TATCTGTTCT TTAGCATCCG AGAGTGGTAA TCCTGATTTG 
ATTTATTCAT TCTTGAGCAT AGCAGGTAAC AGTTCTCTCT GGCAAGCCCG TAAGGGATTG 
GCATCTGGTA TAAGTTATTT AGGTATTCCC GAGGATCAAA AAAGAAAGAC GTTTTCATTT 
GATACTTCTA AAAGCTCTTC ATTATTAAAA AAGCTTTATA GATTTAAACA TGACCCTAAT 
CCTGATGTTG CCAAAACAAT GGGTGAAATT TGGGACACAT TAGTACCTTC AGATCTCAAT 
TTGGCATCTC ATCGTAAATA CTTAGTGGAG GATTGCCTCG AGTTTATGGG GAGCAGATCT 
TGGAGAGATA GGGAATCCAG TGTAAACACT TTGGTTAGTC TTCTTTCTAA TGTTCCTGTG 
ACCGAGTATC TAAATCAATT AGAGGATATA TGGAACATGT CTTTTCGAAC TCTTGATGAT 
ATTAAAGAGA GCGTTAGGGA AGCGTCCTTT CCACTTTGTA AGCTTTTAGC AAGATCAGTT 
ATTCAATCTT TGGAGAAGAC TTCACACAAT ACTAGCCCAA GCGGAATTTG CAAAGGCAAA 
CGGATTGTTT CAGTAGCTTT ACCATTTCTA TTAAAACACG CTTATGATCA GGCTAAAGAA 
GTTCGTTCAC TTACATACTC TACCATCACT GAACTTGTGC GTACTGGCAA TTCTACTTTG 
ACTTCGTTTG TGCCGGCCAT TATGCAGGTT ATGCTTGAAT ATTTGACAGA GTATGAATCA 
AAAGCTGCTA CCTTTTTGGA TTTTCACGCC AAAAATTACA GCATTAAACA AGAAAATATT 
GACAACGCCA GAACGTCAGC CGTTCAGAGC TCTTCAATGA TGGATACGCT AGAGAAGTGT 
ATCGGCCTTT TGGATGAAAG CTCAATGCAA ACATTATATC CAATATTAAA TAGAATGATT 
GCTAAACCTG GGGGTGTACC TACCAAAATA GGCAGCGCCC AAGTGGTGAT GCTTTTAGTA 
ATTCGTCGTG GACCACTCGT CAAGCAGTTT GCTTCAAAGC TGCTTCAATC CTTGAAATCT 
TCTTGCTTTG ATAGAAATGC TGCAGTAAGT GATGCATTTG CATCTGCTAT TGGCTATTTG 
TTACGAGTTT GCCCGTTGGA AATAGCTTCA CAAACTTGTC AAGAAATCAT CGATAAATTC 
TACGATGGAA ATACCAACGA ACAAATAATC AGTTCCAAAT TGACCGTGTA TGCGTCGAGA 
TACGCACCCG ACGTCTTCCT TAATCTTGGT TCTCTTTTTT TTCCTTTCAT TTTTTTTGGG 
AAACATTCTT CTTCCATTTC GATAAATGGG GTTTTAAGTA AAGCATGGGA TGAACTTTCA 
TCTGCCGGAA GCAGTGTGAA CCTGTATTCG GAAGAAATTA TCCTTCTTAT CCAAAAAAAC 
TTAATTGTTA CAAAATGGGA TGTCAAACGT CCTGCTGCTG CTGCATTATT GGAATTTGTC 
AATACTTCGC GACTCACTTA TCGACAAAAT GATATCTACG TGCTTTTAAA TGAAACAATG 
AAAGATAAAA GCTGGCCTGG GAAGGAATTA CTGCTTGAGG CTTATGTTAA ATTTTTAATT 
AAATATCCTG AGTTCATTAA ATCGCAAAAA ATGGAAGAAG TTCACCAAGT GATTGTTAGA 
GAATTTAAGA GACGCAATAT TGTTTACAAA AGCCATGCAA TGGAAAGTGT TGGTGAACTG 
TTATCTGATG AAAATTATAG AGAGCTTGAT TTGTATGAAC TATCATTAAA TGAATGTGGT 
ACATTTTTGC AGAAGGAATG GTTTGATAAA GATGATGAAT TGAATCTTGA GGAAAAAATT 
GCGCTTCAGC GCAATAGCGT CTATGCCATG TTTAATTCCA GCAGACCTGG AAATAAGAAT 
TGTAATGAGA TGTTGCTAAC TTACTTGTCA AATGCCCTTG ATGAAAATTA TTTACATTGG 
AATGTGAAAT TAGCAATTCT GAAAAATGCT CCTCACTTAA AAAAGATAAT GTCTAACGAG 
GAATTCTTAC TGTATAAAGA TATTCTTTAT CGTTGTTACG AAGACAATCC GTCACCAAAA 
GCAAAAGATT ATGCTGAAGT CATCTTCGGC GAAAATTATT TAAGTGTTTT AAGAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ecm29 ( XM_001713058.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713110.1 Schizosaccharomyces pombe 972h- proteasome component Ecm29 (ecm29), mRNA.

Price & Availability↑

CloneID	OSj04908
Clone ID Related Accession (Same CDS sequence)	XM_001713058.2
Accession Version	XM_001713058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	proteasome component Ecm29
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001713058.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGGCTGAAA ATGAATTGAG ATTATTAAAC AATGCAGAGC TGAAGCTCGC TCTAGCGGAA 
AGCGAGGACT CCTTTCAATC GCTTGTGTCA GTTTTTTTAT GTCCGATTTT ACTAAAACTC 
GATTCGCCTC ATGAAAGTGT GAGAAACAAA ACAATTTCAA TTGCAAACCA CATTATGACT 
AGGCTCAACA ATAACGCGCA AGCCATACTA CCTCTTGAAG CCTTAGTAAG CCAGTACGTT 
GAAGCAAATC AACCTTTGCG AAAAAGATTT CTCCTTGCTT TCATATCTAT AGGCGAAAAA 
AGGATACCTT GTTCTGAAAA TTTGGCCCTT CTCCAGATTT GTCTAAATCA TGTCAATGAA 
TACCCATTAG TATTATTGAC ATTGACAATT CGTTTACTTC GTTTTTCAAA GCCAACTTCG 
GCTATTACAT GCTCTAACGA TGTCATTTTA TCTCTTTCTT CATTCTATTT GATTCAGAAA 
GATCAAAGGA TTTTTAGTGA CCTGCAATTT TCCCAAAAAA CAGTAGATTA CCGCTTGAGT 
CTGCTTCGGT GGATTCATTT ATCTAGCTGG CCATCAAATT GGAAGTGGTT GGCTTACTTT 
TTTGCCTCTG CTGATTCTCA TTCTGAAGTT GCCCGACTGG CAGATGAATT TACGCGTGAT 
AGTGGATTAC CTGATCTAGA GAATTTGTCC CACGTGAACG TTCTATTAGA CATAGCGCTT 
GATAAGTTTC GAATCGAAGC TTTGCACGCA AACTTTTCTG TTTCCATTAG CTTAAGGAAC 
AAGGCAATTC AGCATTTACT TAAAAGTAAA ATTGCTGCGA ATACTGACAA AGCTATAAAT 
TGTATTGAAT TCATTCTTGA AGCACCTCCT AGTATGCAAC CCCGGTTAAT ACAGTTTACT 
CGTTGGGTAG TTGACAAAGC TGATCCAAAT TTTCTTAAAC CTAAAGCTGC GATGATATTG 
GAAAAAATTC TTAGCATCTT ATCGAGTAAT ATAATTCAGA GTGATTTACT TAGAGGATTT 
TTATATACAA CGATAGGTTT ACTGACCAAG GTTGATAATC ATCTGATTAC TAATTCTTTG 
CTGACAAATT TATTGACCTC CTTACAATCT GAATTACCAG ACGTACGAGT TTCAATTGAC 
GAAGCTCTTT CTATTATTAT TCCGTACTAT AGTAATTTTC GATTTTCTAA CGAATTGTTG 
CCAGTATTAG AACCCTTTAT TTTTGATTCA CCCGAGTCTC CTGCTGCGTA TTGCGCACTT 
CGATTTGTAT TGGTTGCTTT TCCTTTTGAT TATTTGCCAG CTCGTTTTAT TTGTCTAAAG 
GTACAAAATC CTTTCGTATT TCACCACTCT TTTATTGAAG AAGCTAAAAA AGGACTAAAC 
TTATCTCAAT GGGTTCAGTA TAATAGTGTG TATTCCACGA ATGAAGCTCA AGAGGAAGAT 
AAAGTACGTG CCGCTAGTTA TCCTTCTGCC TCTGAAGTAA TCTCTTTTAT TTTGTCGGAC 
CATGATTTAA AAAAATTTTG GGAATCTAAT GCTGCAGAGT ATTGTTTGGC CATTCTTGAG 
TTTATTGAGA GATGCATTTA TTATTCAGCG GATAGAAGCT TAGAGCTTTA CGACAATGAC 
AAGCTATCTT CTATAGACGC TTTACTGATT CAAGATTCCA AGTTAAGAGA AATGGTGTCT 
GAAAAGTGCA TTTCTTTGTC AAATTTTAAT GTATTTTTAG AATATGTTTT TTATGGTACT 
CTACTCATGC ACTTTGAACC CACTTACGCC CTCAGCAGAT TGGTATCCTT TGCACCACCT 
GAAGTTACAT TTTCTTTACC GGAGTTAGAT TTTCTAACGT CTGTATTTAA CTTTCCACTT 
GCTTTGCGTA ATACTGCAAC ACGAATACTG GGAATAATAC TTAGTACAAA AGATTCAACT 
CGTATATCAG AAGTACTTTC TTCTTGCTTC ACTATTATCT CAACGTCTAA CAATAAAAAT 
GACAATTTTT TCAAGGCTGA GACTGCTCTT CTAATCATCG GCTATACTAT CTCATACTTG 
GCTGCTCAAA CTAATTCAGC AGCCGTCGAT TCTTTCATTC TCAATTCTGG CAGTATAAAA 
GAATTTTTTT CTGTTTTATT GGAATATCTT GGTTCCAATG TTTTGCACAA AAAGACCACT 
TCACTTGCTA TTTATAAAGA ATTGTTTGTT TATTTTACTA GAGACTGGAT TACTTCGTAT 
GGCGTTGACT TTGACGAAAT TTTAAATGTT TTGCTACGCT TTCTAAAAGA GGTTGAAGAT 
ACCAACGTAA AGGTCGAATG TTTACACGTT ATATCACGTA TGTCGTTATC ATTTTCAGAT 
GATGAAATGG CAGAAAAGAT TCTTAAAGCC ATCTATGTTA CATATCACAT GGACTCTCCT 
GATATATTGT TTGCGAGTGC TGAAGCCATG TCGATACTTG CTGGTGGACA TAGAAACGTT 
TTCGTGAAAT CCACGTGCCC CATTTTTTTT CAAAAACAAT TGGACAATTA TAAAGCAGAT 
CACTATTGCT TTACCCTTGA TTTCATTTTA ACTGATTGTG TCAATTCTCC AAAACCCTTA 
CTTCGTAGGG CCTCTTCTTT ATGGCTTTTT TACATAGTTC GCTATTGCGA ACCTCAAACT 
TATACAATGA CGCGGCTTAA TGATATTTAT CATTCATTTC TCTCATTTTT GGTTACTCAA 
GATGATTTTG TTCAGGATAC TGCGTCAAGA GGCCTCAAAG CTATGTATGA TGTTTTAGAA 
GGGGACGAAA GAAAATCATT TACTGATAAT CTGATAAGCA CAATTGCTGC TGACCGCGTT 
GATGAAAAAA CCAAAGCTCC TTTGGACGCA GATACTGCCC TTTTCACTAC AAATAAAGGT 
ACTGTAGCCA CCTATAAGGA TATCTGTTCT TTAGCATCCG AGAGTGGTAA TCCTGATTTG 
ATTTATTCAT TCTTGAGCAT AGCAGGTAAC AGTTCTCTCT GGCAAGCCCG TAAGGGATTG 
GCATCTGGTA TAAGTTATTT AGGTATTCCC GAGGATCAAA AAAGAAAGAC GTTTTCATTT 
GATACTTCTA AAAGCTCTTC ATTATTAAAA AAGCTTTATA GATTTAAACA TGACCCTAAT 
CCTGATGTTG CCAAAACAAT GGGTGAAATT TGGGACACAT TAGTACCTTC AGATCTCAAT 
TTGGCATCTC ATCGTAAATA CTTAGTGGAG GATTGCCTCG AGTTTATGGG GAGCAGATCT 
TGGAGAGATA GGGAATCCAG TGTAAACACT TTGGTTAGTC TTCTTTCTAA TGTTCCTGTG 
ACCGAGTATC TAAATCAATT AGAGGATATA TGGAACATGT CTTTTCGAAC TCTTGATGAT 
ATTAAAGAGA GCGTTAGGGA AGCGTCCTTT CCACTTTGTA AGCTTTTAGC AAGATCAGTT 
ATTCAATCTT TGGAGAAGAC TTCACACAAT ACTAGCCCAA GCGGAATTTG CAAAGGCAAA 
CGGATTGTTT CAGTAGCTTT ACCATTTCTA TTAAAACACG CTTATGATCA GGCTAAAGAA 
GTTCGTTCAC TTACATACTC TACCATCACT GAACTTGTGC GTACTGGCAA TTCTACTTTG 
ACTTCGTTTG TGCCGGCCAT TATGCAGGTT ATGCTTGAAT ATTTGACAGA GTATGAATCA 
AAAGCTGCTA CCTTTTTGGA TTTTCACGCC AAAAATTACA GCATTAAACA AGAAAATATT 
GACAACGCCA GAACGTCAGC CGTTCAGAGC TCTTCAATGA TGGATACGCT AGAGAAGTGT 
ATCGGCCTTT TGGATGAAAG CTCAATGCAA ACATTATATC CAATATTAAA TAGAATGATT 
GCTAAACCTG GGGGTGTACC TACCAAAATA GGCAGCGCCC AAGTGGTGAT GCTTTTAGTA 
ATTCGTCGTG GACCACTCGT CAAGCAGTTT GCTTCAAAGC TGCTTCAATC CTTGAAATCT 
TCTTGCTTTG ATAGAAATGC TGCAGTAAGT GATGCATTTG CATCTGCTAT TGGCTATTTG 
TTACGAGTTT GCCCGTTGGA AATAGCTTCA CAAACTTGTC AAGAAATCAT CGATAAATTC 
TACGATGGAA ATACCAACGA ACAAATAATC AGTTCCAAAT TGACCGTGTA TGCGTCGAGA 
TACGCACCCG ACGTCTTCCT TAATCTTGGT TCTCTTTTTT TTCCTTTCAT TTTTTTTGGG 
AAACATTCTT CTTCCATTTC GATAAATGGG GTTTTAAGTA AAGCATGGGA TGAACTTTCA 
TCTGCCGGAA GCAGTGTGAA CCTGTATTCG GAAGAAATTA TCCTTCTTAT CCAAAAAAAC 
TTAATTGTTA CAAAATGGGA TGTCAAACGT CCTGCTGCTG CTGCATTATT GGAATTTGTC 
AATACTTCGC GACTCACTTA TCGACAAAAT GATATCTACG TGCTTTTAAA TGAAACAATG 
AAAGATAAAA GCTGGCCTGG GAAGGAATTA CTGCTTGAGG CTTATGTTAA ATTTTTAATT 
AAATATCCTG AGTTCATTAA ATCGCAAAAA ATGGAAGAAG TTCACCAAGT GATTGTTAGA 
GAATTTAAGA GACGCAATAT TGTTTACAAA AGCCATGCAA TGGAAAGTGT TGGTGAACTG 
TTATCTGATG AAAATTATAG AGAGCTTGAT TTGTATGAAC TATCATTAAA TGAATGTGGT 
ACATTTTTGC AGAAGGAATG GTTTGATAAA GATGATGAAT TGAATCTTGA GGAAAAAATT 
GCGCTTCAGC GCAATAGCGT CTATGCCATG TTTAATTCCA GCAGACCTGG AAATAAGAAT 
TGTAATGAGA TGTTGCTAAC TTACTTGTCA AATGCCCTTG ATGAAAATTA TTTACATTGG 
AATGTGAAAT TAGCAATTCT GAAAAATGCT CCTCACTTAA AAAAGATAAT GTCTAACGAG 
GAATTCTTAC TGTATAAAGA TATTCTTTAT CGTTGTTACG AAGACAATCC GTCACCAAAA 
GCAAAAGATT ATGCTGAAGT CATCTTCGGC GAAAATTATT TAAGTGTTTT AAGAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713110.1
CDS	146..5185
Translation	MAENELRLLNNAELKLALAESEDSFQSLVSVFLCPILLKLDSPHESVRNKTISIANHIMTRLNNNAQAILPLEALVSQYVEANQPLRKRFLLAFISIGEKRIPCSENLALLQICLNHVNEYPLVLLTLTIRLLRFSKPTSAITCSNDVILSLSSFYLIQKDQRIFSDLQFSQKTVDYRLSLLRWIHLSSWPSNWKWLAYFFASADSHSEVARLADEFTRDSGLPDLENLSHVNVLLDIALDKFRIEALHANFSVSISLRNKAIQHLLKSKIAANTDKAINCIEFILEAPPSMQPRLIQFTRWVVDKADPNFLKPKAAMILEKILSILSSNIIQSDLLRGFLYTTIGLLTKVDNHLITNSLLTNLLTSLQSELPDVRVSIDEALSIIIPYYSNFRFSNELLPVLEPFIFDSPESPAAYCALRFVLVAFPFDYLPARFICLKVQNPFVFHHSFIEEAKKGLNLSQWVQYNSVYSTNEAQEEDKVRAASYPSASEVISFILSDHDLKKFWESNAAEYCLAILEFIERCIYYSADRSLELYDNDKLSSIDALLIQDSKLREMVSEKCISLSNFNVFLEYVFYGTLLMHFEPTYALSRLVSFAPPEVTFSLPELDFLTSVFNFPLALRNTATRILGIILSTKDSTRISEVLSSCFTIISTSNNKNDNFFKAETALLIIGYTISYLAAQTNSAAVDSFILNSGSIKEFFSVLLEYLGSNVLHKKTTSLAIYKELFVYFTRDWITSYGVDFDEILNVLLRFLKEVEDTNVKVECLHVISRMSLSFSDDEMAEKILKAIYVTYHMDSPDILFASAEAMSILAGGHRNVFVKSTCPIFFQKQLDNYKADHYCFTLDFILTDCVNSPKPLLRRASSLWLFYIVRYCEPQTYTMTRLNDIYHSFLSFLVTQDDFVQDTASRGLKAMYDVLEGDERKSFTDNLISTIAADRVDEKTKAPLDADTALFTTNKGTVATYKDICSLASESGNPDLIYSFLSIAGNSSLWQARKGLASGISYLGIPEDQKRKTFSFDTSKSSSLLKKLYRFKHDPNPDVAKTMGEIWDTLVPSDLNLASHRKYLVEDCLEFMGSRSWRDRESSVNTLVSLLSNVPVTEYLNQLEDIWNMSFRTLDDIKESVREASFPLCKLLARSVIQSLEKTSHNTSPSGICKGKRIVSVALPFLLKHAYDQAKEVRSLTYSTITELVRTGNSTLTSFVPAIMQVMLEYLTEYESKAATFLDFHAKNYSIKQENIDNARTSAVQSSSMMDTLEKCIGLLDESSMQTLYPILNRMIAKPGGVPTKIGSAQVVMLLVIRRGPLVKQFASKLLQSLKSSCFDRNAAVSDAFASAIGYLLRVCPLEIASQTCQEIIDKFYDGNTNEQIISSKLTVYASRYAPDVFLNLGSLFFPFIFFGKHSSSISINGVLSKAWDELSSAGSSVNLYSEEIILLIQKNLIVTKWDVKRPAAAALLEFVNTSRLTYRQNDIYVLLNETMKDKSWPGKELLLEAYVKFLIKYPEFIKSQKMEEVHQVIVREFKRRNIVYKSHAMESVGELLSDENYRELDLYELSLNECGTFLQKEWFDKDDELNLEEKIALQRNSVYAMFNSSRPGNKNCNEMLLTYLSNALDENYLHWNVKLAILKNAPHLKKIMSNEEFLLYKDILYRCYEDNPSPKAKDYAEVIFGENYLSVLRN

Target ORF information:

RefSeq Version	XM_001713058.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- proteasome component Ecm29 (ecm29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713058.2

ORF Insert Sequence:

ATGGCTGAAA ATGAATTGAG ATTATTAAAC AATGCAGAGC TGAAGCTCGC TCTAGCGGAA 
AGCGAGGACT CCTTTCAATC GCTTGTGTCA GTTTTTTTAT GTCCGATTTT ACTAAAACTC 
GATTCGCCTC ATGAAAGTGT GAGAAACAAA ACAATTTCAA TTGCAAACCA CATTATGACT 
AGGCTCAACA ATAACGCGCA AGCCATACTA CCTCTTGAAG CCTTAGTAAG CCAGTACGTT 
GAAGCAAATC AACCTTTGCG AAAAAGATTT CTCCTTGCTT TCATATCTAT AGGCGAAAAA 
AGGATACCTT GTTCTGAAAA TTTGGCCCTT CTCCAGATTT GTCTAAATCA TGTCAATGAA 
TACCCATTAG TATTATTGAC ATTGACAATT CGTTTACTTC GTTTTTCAAA GCCAACTTCG 
GCTATTACAT GCTCTAACGA TGTCATTTTA TCTCTTTCTT CATTCTATTT GATTCAGAAA 
GATCAAAGGA TTTTTAGTGA CCTGCAATTT TCCCAAAAAA CAGTAGATTA CCGCTTGAGT 
CTGCTTCGGT GGATTCATTT ATCTAGCTGG CCATCAAATT GGAAGTGGTT GGCTTACTTT 
TTTGCCTCTG CTGATTCTCA TTCTGAAGTT GCCCGACTGG CAGATGAATT TACGCGTGAT 
AGTGGATTAC CTGATCTAGA GAATTTGTCC CACGTGAACG TTCTATTAGA CATAGCGCTT 
GATAAGTTTC GAATCGAAGC TTTGCACGCA AACTTTTCTG TTTCCATTAG CTTAAGGAAC 
AAGGCAATTC AGCATTTACT TAAAAGTAAA ATTGCTGCGA ATACTGACAA AGCTATAAAT 
TGTATTGAAT TCATTCTTGA AGCACCTCCT AGTATGCAAC CCCGGTTAAT ACAGTTTACT 
CGTTGGGTAG TTGACAAAGC TGATCCAAAT TTTCTTAAAC CTAAAGCTGC GATGATATTG 
GAAAAAATTC TTAGCATCTT ATCGAGTAAT ATAATTCAGA GTGATTTACT TAGAGGATTT 
TTATATACAA CGATAGGTTT ACTGACCAAG GTTGATAATC ATCTGATTAC TAATTCTTTG 
CTGACAAATT TATTGACCTC CTTACAATCT GAATTACCAG ACGTACGAGT TTCAATTGAC 
GAAGCTCTTT CTATTATTAT TCCGTACTAT AGTAATTTTC GATTTTCTAA CGAATTGTTG 
CCAGTATTAG AACCCTTTAT TTTTGATTCA CCCGAGTCTC CTGCTGCGTA TTGCGCACTT 
CGATTTGTAT TGGTTGCTTT TCCTTTTGAT TATTTGCCAG CTCGTTTTAT TTGTCTAAAG 
GTACAAAATC CTTTCGTATT TCACCACTCT TTTATTGAAG AAGCTAAAAA AGGACTAAAC 
TTATCTCAAT GGGTTCAGTA TAATAGTGTG TATTCCACGA ATGAAGCTCA AGAGGAAGAT 
AAAGTACGTG CCGCTAGTTA TCCTTCTGCC TCTGAAGTAA TCTCTTTTAT TTTGTCGGAC 
CATGATTTAA AAAAATTTTG GGAATCTAAT GCTGCAGAGT ATTGTTTGGC CATTCTTGAG 
TTTATTGAGA GATGCATTTA TTATTCAGCG GATAGAAGCT TAGAGCTTTA CGACAATGAC 
AAGCTATCTT CTATAGACGC TTTACTGATT CAAGATTCCA AGTTAAGAGA AATGGTGTCT 
GAAAAGTGCA TTTCTTTGTC AAATTTTAAT GTATTTTTAG AATATGTTTT TTATGGTACT 
CTACTCATGC ACTTTGAACC CACTTACGCC CTCAGCAGAT TGGTATCCTT TGCACCACCT 
GAAGTTACAT TTTCTTTACC GGAGTTAGAT TTTCTAACGT CTGTATTTAA CTTTCCACTT 
GCTTTGCGTA ATACTGCAAC ACGAATACTG GGAATAATAC TTAGTACAAA AGATTCAACT 
CGTATATCAG AAGTACTTTC TTCTTGCTTC ACTATTATCT CAACGTCTAA CAATAAAAAT 
GACAATTTTT TCAAGGCTGA GACTGCTCTT CTAATCATCG GCTATACTAT CTCATACTTG 
GCTGCTCAAA CTAATTCAGC AGCCGTCGAT TCTTTCATTC TCAATTCTGG CAGTATAAAA 
GAATTTTTTT CTGTTTTATT GGAATATCTT GGTTCCAATG TTTTGCACAA AAAGACCACT 
TCACTTGCTA TTTATAAAGA ATTGTTTGTT TATTTTACTA GAGACTGGAT TACTTCGTAT 
GGCGTTGACT TTGACGAAAT TTTAAATGTT TTGCTACGCT TTCTAAAAGA GGTTGAAGAT 
ACCAACGTAA AGGTCGAATG TTTACACGTT ATATCACGTA TGTCGTTATC ATTTTCAGAT 
GATGAAATGG CAGAAAAGAT TCTTAAAGCC ATCTATGTTA CATATCACAT GGACTCTCCT 
GATATATTGT TTGCGAGTGC TGAAGCCATG TCGATACTTG CTGGTGGACA TAGAAACGTT 
TTCGTGAAAT CCACGTGCCC CATTTTTTTT CAAAAACAAT TGGACAATTA TAAAGCAGAT 
CACTATTGCT TTACCCTTGA TTTCATTTTA ACTGATTGTG TCAATTCTCC AAAACCCTTA 
CTTCGTAGGG CCTCTTCTTT ATGGCTTTTT TACATAGTTC GCTATTGCGA ACCTCAAACT 
TATACAATGA CGCGGCTTAA TGATATTTAT CATTCATTTC TCTCATTTTT GGTTACTCAA 
GATGATTTTG TTCAGGATAC TGCGTCAAGA GGCCTCAAAG CTATGTATGA TGTTTTAGAA 
GGGGACGAAA GAAAATCATT TACTGATAAT CTGATAAGCA CAATTGCTGC TGACCGCGTT 
GATGAAAAAA CCAAAGCTCC TTTGGACGCA GATACTGCCC TTTTCACTAC AAATAAAGGT 
ACTGTAGCCA CCTATAAGGA TATCTGTTCT TTAGCATCCG AGAGTGGTAA TCCTGATTTG 
ATTTATTCAT TCTTGAGCAT AGCAGGTAAC AGTTCTCTCT GGCAAGCCCG TAAGGGATTG 
GCATCTGGTA TAAGTTATTT AGGTATTCCC GAGGATCAAA AAAGAAAGAC GTTTTCATTT 
GATACTTCTA AAAGCTCTTC ATTATTAAAA AAGCTTTATA GATTTAAACA TGACCCTAAT 
CCTGATGTTG CCAAAACAAT GGGTGAAATT TGGGACACAT TAGTACCTTC AGATCTCAAT 
TTGGCATCTC ATCGTAAATA CTTAGTGGAG GATTGCCTCG AGTTTATGGG GAGCAGATCT 
TGGAGAGATA GGGAATCCAG TGTAAACACT TTGGTTAGTC TTCTTTCTAA TGTTCCTGTG 
ACCGAGTATC TAAATCAATT AGAGGATATA TGGAACATGT CTTTTCGAAC TCTTGATGAT 
ATTAAAGAGA GCGTTAGGGA AGCGTCCTTT CCACTTTGTA AGCTTTTAGC AAGATCAGTT 
ATTCAATCTT TGGAGAAGAC TTCACACAAT ACTAGCCCAA GCGGAATTTG CAAAGGCAAA 
CGGATTGTTT CAGTAGCTTT ACCATTTCTA TTAAAACACG CTTATGATCA GGCTAAAGAA 
GTTCGTTCAC TTACATACTC TACCATCACT GAACTTGTGC GTACTGGCAA TTCTACTTTG 
ACTTCGTTTG TGCCGGCCAT TATGCAGGTT ATGCTTGAAT ATTTGACAGA GTATGAATCA 
AAAGCTGCTA CCTTTTTGGA TTTTCACGCC AAAAATTACA GCATTAAACA AGAAAATATT 
GACAACGCCA GAACGTCAGC CGTTCAGAGC TCTTCAATGA TGGATACGCT AGAGAAGTGT 
ATCGGCCTTT TGGATGAAAG CTCAATGCAA ACATTATATC CAATATTAAA TAGAATGATT 
GCTAAACCTG GGGGTGTACC TACCAAAATA GGCAGCGCCC AAGTGGTGAT GCTTTTAGTA 
ATTCGTCGTG GACCACTCGT CAAGCAGTTT GCTTCAAAGC TGCTTCAATC CTTGAAATCT 
TCTTGCTTTG ATAGAAATGC TGCAGTAAGT GATGCATTTG CATCTGCTAT TGGCTATTTG 
TTACGAGTTT GCCCGTTGGA AATAGCTTCA CAAACTTGTC AAGAAATCAT CGATAAATTC 
TACGATGGAA ATACCAACGA ACAAATAATC AGTTCCAAAT TGACCGTGTA TGCGTCGAGA 
TACGCACCCG ACGTCTTCCT TAATCTTGGT TCTCTTTTTT TTCCTTTCAT TTTTTTTGGG 
AAACATTCTT CTTCCATTTC GATAAATGGG GTTTTAAGTA AAGCATGGGA TGAACTTTCA 
TCTGCCGGAA GCAGTGTGAA CCTGTATTCG GAAGAAATTA TCCTTCTTAT CCAAAAAAAC 
TTAATTGTTA CAAAATGGGA TGTCAAACGT CCTGCTGCTG CTGCATTATT GGAATTTGTC 
AATACTTCGC GACTCACTTA TCGACAAAAT GATATCTACG TGCTTTTAAA TGAAACAATG 
AAAGATAAAA GCTGGCCTGG GAAGGAATTA CTGCTTGAGG CTTATGTTAA ATTTTTAATT 
AAATATCCTG AGTTCATTAA ATCGCAAAAA ATGGAAGAAG TTCACCAAGT GATTGTTAGA 
GAATTTAAGA GACGCAATAT TGTTTACAAA AGCCATGCAA TGGAAAGTGT TGGTGAACTG 
TTATCTGATG AAAATTATAG AGAGCTTGAT TTGTATGAAC TATCATTAAA TGAATGTGGT 
ACATTTTTGC AGAAGGAATG GTTTGATAAA GATGATGAAT TGAATCTTGA GGAAAAAATT 
GCGCTTCAGC GCAATAGCGT CTATGCCATG TTTAATTCCA GCAGACCTGG AAATAAGAAT 
TGTAATGAGA TGTTGCTAAC TTACTTGTCA AATGCCCTTG ATGAAAATTA TTTACATTGG 
AATGTGAAAT TAGCAATTCT GAAAAATGCT CCTCACTTAA AAAAGATAAT GTCTAACGAG 
GAATTCTTAC TGTATAAAGA TATTCTTTAT CGTTGTTACG AAGACAATCC GTCACCAAAA 
GCAAAAGATT ATGCTGAAGT CATCTTCGGC GAAAATTATT TAAGTGTTTT AAGAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ecm29 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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