mak1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mak1
Entrez Gene ID	2542119
Full Name	histidine kinase Mak1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020116.2	NP_594687.1	histidine kinase Mak1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mak1	NP_594687.1
Magnaporthe oryzae (rice blast fungus)	MGG_11882	XP_003709348.1
Neurospora crassa	NCU01833	XP_956617.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate distinct functions for the three histidine kinases; Mak1 alone or Mak2 and Mak3 together were sufficient for the repression of the meiotic cycle when nutrients were available.
Title: Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.

The following mak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103392	NM_001020116.2 Latest version!	Schizosaccharomyces pombe histidine kinase Mak1 (mak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mak1 ( NM_001020116.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594687.1 Schizosaccharomyces pombe histidine kinase Mak1 (mak1), mRNA.

Price & Availability↑

CloneID	OSc103392
Clone ID Related Accession (Same CDS sequence)	NM_001020116.2
Accession Version	NM_001020116.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	histidine kinase Mak1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020116.1.

ATGAGGCCAC CTGACGATCA AATCAACAAT AACGTTGGTT CTAATTCTCA CTTGGAAAAG 
TTGAAAGAGG CCATGGACCA CCAGCTGCAA AAATCATCAA AAATTGTAGG ATCGTTTACT 
AATTCTCAAA ACTCTTCTGT TGGCTCTGTT CATTCTCCGA TACTTGAATC TCCCACCAGT 
TTAAATAGGC AGCATCGAAA TTCATTTTCT TTCAATAATG TATCTTCTCC TTCACTAGAG 
GATGAGCGAC TTATTAATTT TCCCCGAGTA AATCCAAATC GATTGATGAC ATCCAAACGT 
CCCAATGAGT TATTTAAAAC CTCCTCAATG AGTTCAGATT GCTATTCTCC TCAAAAATCT 
AGGGAATCAC TAAATTCATT ATGCCATTCA CCTGCTCCAT CCGTTTCTTC TTGTGGAAAT 
GCTTTGAACA ACGATAATAC TTCTGCTTCG CACTCACTAA CTGATGAGCA ACCATTTGAA 
ACAGATTCAT CCGCTAACTT ATTTAAGCAG TTACAGGAGA AGCGTAACCG TACCATTGGA 
AATGTGTATG AAATGGCTTG CTTATTGGTC TTTAAAACCG GTTTGATGAA TTTCTGGAAA 
AACATTATTG ACTTTTTCGC TCAGCAGTTT TTTTCCACTC AAATATCTGT TGTAGAACCG 
CGTGACCTTT CTGACATATA CAATACTCCT TGGCAACTCA GATGTTATTA CGATGGCGGT 
TCCCATTATG ATCCGTATAG TAACCCTATA AGTGTTAATG ACAACCTTGC TAGCAGTTCT 
TATGTTACCG TAGTTGCTTC GGATGGTTCA AAGGGTATTA TATACAAAGA TCCAGCTTCT 
CTTAAACATG AAGGGGATTT GCTTATTGAT AGCAAAGTTG TACAAACAGT CTTGGAGCGT 
GCGACATTGC TTGTATATAC ACGTAAACAA CAGCACATTG TTAAAAACAC CAAGGTTCAT 
GATAATGATT ACTTTAGTTC TATACCTAAT GTTGATGATA TTCGCAGCAT TAAGAATTCA 
TGGAAAGTTT TTCATGATGA AAAACTTAAT GAATTGAAAA AGCAGGTTGA AATTAGTGCT 
TCTGCAGCTC AGTTAAATGG ACTTTATCCA CAGAAAAAGA GAGCATTTGT TTCACATTTC 
AGTCAGAATC GTAAACCGTA TTCCCAAAGT GACATTTCAA AAGCACAAAG TTCGTCTTTT 
TCGGAAGAAC CTTCAAACAT TTATGATGAG TATGAACAAA ATTTACTTTC TCCTTGGTCA 
AGATCTCCAG TTGCTAGTCC CTCCATTCAA ACAGATCCTA ATAGGAATCC ATTCTTCCAA 
AATTGCTTGC AAGAATCTTC TTTCGCTACT GAATCGTCAA CAGAGAAGTC TGCTTCAGAG 
TCCGTATCAG AAACAGCTGT TAATGATGAT TGTAAAGGTA TGAATTTTTC TGGTAACAGG 
CGTCAAGAAG ATCATTTGAA CGACTTCACC AGTTTTCCTA CTGAAACTGC TGTCAGTATT 
GTACATGTTC CTCTGATGTT TCCTTGTTCG GATCAAACTT CAAGCCGTGG GAGAGCTCCA 
ATTGCAATTC TTTCTTTCAA GTCCAATTTA GTTCCTTATC CGGAAAATTT AATAGCCTCC 
ATAGAACGTT TGATACCCTT TATTTTTTCT TCATACTCAA ATTCTCAATC TGTTCCGTTA 
CTTCCTTGTC CTACACAAAG GCATCTATTA TTTAACACGT CTAGCACTGA CAATACCAAG 
GAGTTGAGTA TGAGCGCGAG CTCCGAAAAC TCTGATTGTC CTCATAAAGA AGGAGAGTGC 
GTAGGCAGCT TTTGCAATAT CAATGCTAAA GGATCTTCTC TTAATAACAT ACCTAAATTG 
CCTAGGTTTG TACCAGTTCC TTCTGAATTT TTTAAAAAAA ACCAGCGATC ATGGGTTACT 
TTAAAGAAGC ATCGTTTGCT AGCTAGATTG AAGTCTCGAA TTAGCAAAAA GAATTCTAAA 
GTGAACGAGA ATTTGAGATT CTCGCTAAAT GATGGTGAAA ATTATTCAAA TGAAACTATT 
ACTCTAAAGA AGGATGAAAT TGTTTTAGAT AAATCAAAAT CATATGCCTG TTGCACTTCT 
GAATCTCACA AGTATGTGCA AGGGCATTGT GGTGGTCAAG CGCCTCCTTT TCCCTTACTA 
AAGGTTATAA TTGATTCTAT ACCGGTTCAT GTGTTTACTG CGGATCCGGG AAGTGGAAAA 
CTCACATGGG TTAATAGAAA AACTCTTCTT TACTGTGGTT TAAATATGAA TGAGCAAATA 
GAGCTACAAT TTAGTCGAAT TCATCCTGAT GATCTGCCAA ACTTTTTAAA TGACTGGAAA 
TCTTCATTAT TCTCTGGTAG TGGTTTTTAT CATGAAATTC GTTTGCAAAG GTTTGATAAT 
GTTTATCGAT ACTTTATCTG TCGCGCGGTT CCTTTACGGG ATTGCACTGG ATCTGTGCTA 
CATTTTTTTG GAACAATGAC GGATGTCCAT GATCAAAAGT TGGCAGAACG AGAACTACAA 
AAACAATCAG CTATAGCCGC AAATGAAAAC AGCTACAGGT CTTTAGCTGA AGCTTCTCCT 
CAAATTGTTT TTGCCGCAAA TGGTAAAAAT GGAATTATTT ATGCAAATGC GCAGTGGTTA 
AGTTATTCAG GTCTTTCACT GGAATCTTCA TTGGGACTTG GGTTTTTATC TGCTGTATAT 
CACGCTGATC GCAAGAAATG TTTATTGCCT GAATCTTTGG AGGGAACGTT TAATAACCAA 
GACGAAAGTA ATGGTACCAA AACGTTTGCG GCGGAGATAC GTTTTAGATC TACCGATGGT 
CATTATAGAT GGCATTTGGT GAAATCTGTT TGCGTAAATA ATTCTGCTGA TACGTCTACT 
AATCTCTGGT TAGGAACTTG TACTGATATT CACGATCATA AAATGTTGGA AGAAAAGCTC 
CAAGAATCTA ACATTGAAGC TCAAAGAATT GTTCGGAGCA AAATGCAGTA TCTTTCCAAT 
ATGTCTCATG AAATTCGAAC CCCTCTTATC GGTATTACAG GCATGGTAAG CTTCTTGTTG 
GAAACTCAAA TGTCTGCCGA ACAGCTGAGT TATGCACGTA TTATTCAGCA ATCAGCTAAG 
TCTCTTTTGA CTGTTATCAA TGATATTTTG GACCTTAGTA AAGTCAGAGC TGGAATGATG 
AAGCTAACTA GCCAACGCTT TTCTGTACGA GCTATGATGG AAGATGCAAA CGAAACTCTA 
GGTACCCTCG CTTTTTCAAA GGGAATAGAA TTGAACTACA CGGTTGACAT TGATGTTCCT 
GATATAGTAT TTGGGGATAA TATGAGAATG AGGCAAGTTG CTTTGAATGT GATCGGAAAT 
GCTATTAAAT TTACGAATGT TGGTGAAGTT TTTACTCGTT GTTCCGTTGA AAAGATTGAT 
TACTCAACCA ATACTGTTGT TTTAAAGTGG GAATGCATTG ATACCGGTCA AGGGTTTAAC 
AGAGATGATC AATTACAAAT GTTTAAACCG TTTTCTCAAG TAGAGAGTTC TACATTACCA 
AGACATGGTG GCTCAGGTCT TGGATTAGTT ATTTCAAAAG AGCTTGTTGA GCTACATAAT 
GGTAGTATGT CCTGTCAAAG TAGAAGAGGC GTGGGAACGC GCTTTATGTG GACTGCAACG 
TTTACAATGG ATAAAACTCC TCTAAAATTT GAACCCCCAG ATGGTTGTTG TCCAGTCTGT 
TTTTGTCCAT ACGAAAAAAG CAAACAAAGC ACAGAAGACT ATTATTGCGC AGACGATGGA 
AACGATAAAA GCGCGACGAA TTTTGTAAAA TTGGCCGTAA ATAAAGCAGA TCCCGGAAGA 
GAAAGCAACC GACGTAAACT TGAATCGGAC AAAAATGTTC AATCCAACAA ATATGTGAAT 
CCTTTCGCTT CTGAATCGGA ATTTTGTCGA TGCGGCGCAT CTGCTGATCC ATACACAGTT 
CTATTTTGGA GACTCTATAG AAACAAACCT TCTGGGATCA AGTTGGATAA AAGTGCTTTA 
GCCGTTGTTG TATCACACAC TAAATACAGT AGTGAAGCGA TTGGCAACAT GCTTCAATCA 
ATTATCGATA TAAGCTCATT TAAAGATATC GTAAGGTATG GAAATACCTA TGAAGCCTTT 
GAAGAATTGC TAGAGAATCC TATGCAATCC AAGGTCACCC ATATTATATT AAATCTTCCG 
GACATAGAAG CATATGTTTT ATTTGTCAAA TCACTGCAAC TTTGTAGTCT ATACAAGGAT 
ACAAAATTTA TTTTGGTGAC TTCCACTCGA CAAAAAGAAT CATTATCTAA GATTTTTTCA 
GATAGCGAGG ATTGTAATTC GGAAAGCATC CATTACGTTT TAAAACTTGT GAAACCATCC 
AAGTTTTTTC CATTATTTTA TTCTGATTCT GAGGAAAAGG GGAAAATCGG TGCACTTAAT 
GATATGACTC GAAAGGCTGC AATGGAGCAG AAGGCTGATG CTGAAACACT CCGATATAAT 
CTGGCAAAGT CTGGCTTTAG CGTGTTGTTG GCGGAAGACA ACATTATCAA TATTAAGGTT 
ATAAGCCGTT ACCTTGAAAG AATTGGTGTC AAATTCAAGG TCACCATGGA CGGTTTGCAA 
TGTGTTGAAG AATGGAAACG TGAAAAGCCT AATTTTTACT CTCTTATTCT AATGGATTTA 
CAAATGCCTG TTATGGATGG TTACCAGGCA TGTAATGAAA TTCGTAAATA CGAATTGGAA 
AACGATTACC CTAAAGTTCC TATAGTTGCA CTTAGTGCGA ATGCTTTACC TCATGTTGTT 
TTAAGCTGCA AAGATAGTGG TTTTGATTCT TACCTTGCCA AACCTATTAC TTTGCAACAC 
TTGTCCTTAA TCATATCTGG CATACTTAAT TATACGAACC AATCAAAGTT ACACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594687.1
CDS	244..5163
Translation	MRPPDDQINNNVGSNSHLEKLKEAMDHQLQKSSKIVGSFTNSQNSSVGSVHSPILESPTSLNRQHRNSFSFNNVSSPSLEDERLINFPRVNPNRLMTSKRPNELFKTSSMSSDCYSPQKSRESLNSLCHSPAPSVSSCGNALNNDNTSASHSLTDEQPFETDSSANLFKQLQEKRNRTIGNVYEMACLLVFKTGLMNFWKNIIDFFAQQFFSTQISVVEPRDLSDIYNTPWQLRCYYDGGSHYDPYSNPISVNDNLASSSYVTVVASDGSKGIIYKDPASLKHEGDLLIDSKVVQTVLERATLLVYTRKQQHIVKNTKVHDNDYFSSIPNVDDIRSIKNSWKVFHDEKLNELKKQVEISASAAQLNGLYPQKKRAFVSHFSQNRKPYSQSDISKAQSSSFSEEPSNIYDEYEQNLLSPWSRSPVASPSIQTDPNRNPFFQNCLQESSFATESSTEKSASESVSETAVNDDCKGMNFSGNRRQEDHLNDFTSFPTETAVSIVHVPLMFPCSDQTSSRGRAPIAILSFKSNLVPYPENLIASIERLIPFIFSSYSNSQSVPLLPCPTQRHLLFNTSSTDNTKELSMSASSENSDCPHKEGECVGSFCNINAKGSSLNNIPKLPRFVPVPSEFFKKNQRSWVTLKKHRLLARLKSRISKKNSKVNENLRFSLNDGENYSNETITLKKDEIVLDKSKSYACCTSESHKYVQGHCGGQAPPFPLLKVIIDSIPVHVFTADPGSGKLTWVNRKTLLYCGLNMNEQIELQFSRIHPDDLPNFLNDWKSSLFSGSGFYHEIRLQRFDNVYRYFICRAVPLRDCTGSVLHFFGTMTDVHDQKLAERELQKQSAIAANENSYRSLAEASPQIVFAANGKNGIIYANAQWLSYSGLSLESSLGLGFLSAVYHADRKKCLLPESLEGTFNNQDESNGTKTFAAEIRFRSTDGHYRWHLVKSVCVNNSADTSTNLWLGTCTDIHDHKMLEEKLQESNIEAQRIVRSKMQYLSNMSHEIRTPLIGITGMVSFLLETQMSAEQLSYARIIQQSAKSLLTVINDILDLSKVRAGMMKLTSQRFSVRAMMEDANETLGTLAFSKGIELNYTVDIDVPDIVFGDNMRMRQVALNVIGNAIKFTNVGEVFTRCSVEKIDYSTNTVVLKWECIDTGQGFNRDDQLQMFKPFSQVESSTLPRHGGSGLGLVISKELVELHNGSMSCQSRRGVGTRFMWTATFTMDKTPLKFEPPDGCCPVCFCPYEKSKQSTEDYYCADDGNDKSATNFVKLAVNKADPGRESNRRKLESDKNVQSNKYVNPFASESEFCRCGASADPYTVLFWRLYRNKPSGIKLDKSALAVVVSHTKYSSEAIGNMLQSIIDISSFKDIVRYGNTYEAFEELLENPMQSKVTHIILNLPDIEAYVLFVKSLQLCSLYKDTKFILVTSTRQKESLSKIFSDSEDCNSESIHYVLKLVKPSKFFPLFYSDSEEKGKIGALNDMTRKAAMEQKADAETLRYNLAKSGFSVLLAEDNIINIKVISRYLERIGVKFKVTMDGLQCVEEWKREKPNFYSLILMDLQMPVMDGYQACNEIRKYELENDYPKVPIVALSANALPHVVLSCKDSGFDSYLAKPITLQHLSLIISGILNYTNQSKLHK

Target ORF information:

RefSeq Version	NM_001020116.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe histidine kinase Mak1 (mak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020116.2

ORF Insert Sequence:

ATGAGGCCAC CTGACGATCA AATCAACAAT AACGTTGGTT CTAATTCTCA CTTGGAAAAG 
TTGAAAGAGG CCATGGACCA CCAGCTGCAA AAATCATCAA AAATTGTAGG ATCGTTTACT 
AATTCTCAAA ACTCTTCTGT TGGCTCTGTT CATTCTCCGA TACTTGAATC TCCCACCAGT 
TTAAATAGGC AGCATCGAAA TTCATTTTCT TTCAATAATG TATCTTCTCC TTCACTAGAG 
GATGAGCGAC TTATTAATTT TCCCCGAGTA AATCCAAATC GATTGATGAC ATCCAAACGT 
CCCAATGAGT TATTTAAAAC CTCCTCAATG AGTTCAGATT GCTATTCTCC TCAAAAATCT 
AGGGAATCAC TAAATTCATT ATGCCATTCA CCTGCTCCAT CCGTTTCTTC TTGTGGAAAT 
GCTTTGAACA ACGATAATAC TTCTGCTTCG CACTCACTAA CTGATGAGCA ACCATTTGAA 
ACAGATTCAT CCGCTAACTT ATTTAAGCAG TTACAGGAGA AGCGTAACCG TACCATTGGA 
AATGTGTATG AAATGGCTTG CTTATTGGTC TTTAAAACCG GTTTGATGAA TTTCTGGAAA 
AACATTATTG ACTTTTTCGC TCAGCAGTTT TTTTCCACTC AAATATCTGT TGTAGAACCG 
CGTGACCTTT CTGACATATA CAATACTCCT TGGCAACTCA GATGTTATTA CGATGGCGGT 
TCCCATTATG ATCCGTATAG TAACCCTATA AGTGTTAATG ACAACCTTGC TAGCAGTTCT 
TATGTTACCG TAGTTGCTTC GGATGGTTCA AAGGGTATTA TATACAAAGA TCCAGCTTCT 
CTTAAACATG AAGGGGATTT GCTTATTGAT AGCAAAGTTG TACAAACAGT CTTGGAGCGT 
GCGACATTGC TTGTATATAC ACGTAAACAA CAGCACATTG TTAAAAACAC CAAGGTTCAT 
GATAATGATT ACTTTAGTTC TATACCTAAT GTTGATGATA TTCGCAGCAT TAAGAATTCA 
TGGAAAGTTT TTCATGATGA AAAACTTAAT GAATTGAAAA AGCAGGTTGA AATTAGTGCT 
TCTGCAGCTC AGTTAAATGG ACTTTATCCA CAGAAAAAGA GAGCATTTGT TTCACATTTC 
AGTCAGAATC GTAAACCGTA TTCCCAAAGT GACATTTCAA AAGCACAAAG TTCGTCTTTT 
TCGGAAGAAC CTTCAAACAT TTATGATGAG TATGAACAAA ATTTACTTTC TCCTTGGTCA 
AGATCTCCAG TTGCTAGTCC CTCCATTCAA ACAGATCCTA ATAGGAATCC ATTCTTCCAA 
AATTGCTTGC AAGAATCTTC TTTCGCTACT GAATCGTCAA CAGAGAAGTC TGCTTCAGAG 
TCCGTATCAG AAACAGCTGT TAATGATGAT TGTAAAGGTA TGAATTTTTC TGGTAACAGG 
CGTCAAGAAG ATCATTTGAA CGACTTCACC AGTTTTCCTA CTGAAACTGC TGTCAGTATT 
GTACATGTTC CTCTGATGTT TCCTTGTTCG GATCAAACTT CAAGCCGTGG GAGAGCTCCA 
ATTGCAATTC TTTCTTTCAA GTCCAATTTA GTTCCTTATC CGGAAAATTT AATAGCCTCC 
ATAGAACGTT TGATACCCTT TATTTTTTCT TCATACTCAA ATTCTCAATC TGTTCCGTTA 
CTTCCTTGTC CTACACAAAG GCATCTATTA TTTAACACGT CTAGCACTGA CAATACCAAG 
GAGTTGAGTA TGAGCGCGAG CTCCGAAAAC TCTGATTGTC CTCATAAAGA AGGAGAGTGC 
GTAGGCAGCT TTTGCAATAT CAATGCTAAA GGATCTTCTC TTAATAACAT ACCTAAATTG 
CCTAGGTTTG TACCAGTTCC TTCTGAATTT TTTAAAAAAA ACCAGCGATC ATGGGTTACT 
TTAAAGAAGC ATCGTTTGCT AGCTAGATTG AAGTCTCGAA TTAGCAAAAA GAATTCTAAA 
GTGAACGAGA ATTTGAGATT CTCGCTAAAT GATGGTGAAA ATTATTCAAA TGAAACTATT 
ACTCTAAAGA AGGATGAAAT TGTTTTAGAT AAATCAAAAT CATATGCCTG TTGCACTTCT 
GAATCTCACA AGTATGTGCA AGGGCATTGT GGTGGTCAAG CGCCTCCTTT TCCCTTACTA 
AAGGTTATAA TTGATTCTAT ACCGGTTCAT GTGTTTACTG CGGATCCGGG AAGTGGAAAA 
CTCACATGGG TTAATAGAAA AACTCTTCTT TACTGTGGTT TAAATATGAA TGAGCAAATA 
GAGCTACAAT TTAGTCGAAT TCATCCTGAT GATCTGCCAA ACTTTTTAAA TGACTGGAAA 
TCTTCATTAT TCTCTGGTAG TGGTTTTTAT CATGAAATTC GTTTGCAAAG GTTTGATAAT 
GTTTATCGAT ACTTTATCTG TCGCGCGGTT CCTTTACGGG ATTGCACTGG ATCTGTGCTA 
CATTTTTTTG GAACAATGAC GGATGTCCAT GATCAAAAGT TGGCAGAACG AGAACTACAA 
AAACAATCAG CTATAGCCGC AAATGAAAAC AGCTACAGGT CTTTAGCTGA AGCTTCTCCT 
CAAATTGTTT TTGCCGCAAA TGGTAAAAAT GGAATTATTT ATGCAAATGC GCAGTGGTTA 
AGTTATTCAG GTCTTTCACT GGAATCTTCA TTGGGACTTG GGTTTTTATC TGCTGTATAT 
CACGCTGATC GCAAGAAATG TTTATTGCCT GAATCTTTGG AGGGAACGTT TAATAACCAA 
GACGAAAGTA ATGGTACCAA AACGTTTGCG GCGGAGATAC GTTTTAGATC TACCGATGGT 
CATTATAGAT GGCATTTGGT GAAATCTGTT TGCGTAAATA ATTCTGCTGA TACGTCTACT 
AATCTCTGGT TAGGAACTTG TACTGATATT CACGATCATA AAATGTTGGA AGAAAAGCTC 
CAAGAATCTA ACATTGAAGC TCAAAGAATT GTTCGGAGCA AAATGCAGTA TCTTTCCAAT 
ATGTCTCATG AAATTCGAAC CCCTCTTATC GGTATTACAG GCATGGTAAG CTTCTTGTTG 
GAAACTCAAA TGTCTGCCGA ACAGCTGAGT TATGCACGTA TTATTCAGCA ATCAGCTAAG 
TCTCTTTTGA CTGTTATCAA TGATATTTTG GACCTTAGTA AAGTCAGAGC TGGAATGATG 
AAGCTAACTA GCCAACGCTT TTCTGTACGA GCTATGATGG AAGATGCAAA CGAAACTCTA 
GGTACCCTCG CTTTTTCAAA GGGAATAGAA TTGAACTACA CGGTTGACAT TGATGTTCCT 
GATATAGTAT TTGGGGATAA TATGAGAATG AGGCAAGTTG CTTTGAATGT GATCGGAAAT 
GCTATTAAAT TTACGAATGT TGGTGAAGTT TTTACTCGTT GTTCCGTTGA AAAGATTGAT 
TACTCAACCA ATACTGTTGT TTTAAAGTGG GAATGCATTG ATACCGGTCA AGGGTTTAAC 
AGAGATGATC AATTACAAAT GTTTAAACCG TTTTCTCAAG TAGAGAGTTC TACATTACCA 
AGACATGGTG GCTCAGGTCT TGGATTAGTT ATTTCAAAAG AGCTTGTTGA GCTACATAAT 
GGTAGTATGT CCTGTCAAAG TAGAAGAGGC GTGGGAACGC GCTTTATGTG GACTGCAACG 
TTTACAATGG ATAAAACTCC TCTAAAATTT GAACCCCCAG ATGGTTGTTG TCCAGTCTGT 
TTTTGTCCAT ACGAAAAAAG CAAACAAAGC ACAGAAGACT ATTATTGCGC AGACGATGGA 
AACGATAAAA GCGCGACGAA TTTTGTAAAA TTGGCCGTAA ATAAAGCAGA TCCCGGAAGA 
GAAAGCAACC GACGTAAACT TGAATCGGAC AAAAATGTTC AATCCAACAA ATATGTGAAT 
CCTTTCGCTT CTGAATCGGA ATTTTGTCGA TGCGGCGCAT CTGCTGATCC ATACACAGTT 
CTATTTTGGA GACTCTATAG AAACAAACCT TCTGGGATCA AGTTGGATAA AAGTGCTTTA 
GCCGTTGTTG TATCACACAC TAAATACAGT AGTGAAGCGA TTGGCAACAT GCTTCAATCA 
ATTATCGATA TAAGCTCATT TAAAGATATC GTAAGGTATG GAAATACCTA TGAAGCCTTT 
GAAGAATTGC TAGAGAATCC TATGCAATCC AAGGTCACCC ATATTATATT AAATCTTCCG 
GACATAGAAG CATATGTTTT ATTTGTCAAA TCACTGCAAC TTTGTAGTCT ATACAAGGAT 
ACAAAATTTA TTTTGGTGAC TTCCACTCGA CAAAAAGAAT CATTATCTAA GATTTTTTCA 
GATAGCGAGG ATTGTAATTC GGAAAGCATC CATTACGTTT TAAAACTTGT GAAACCATCC 
AAGTTTTTTC CATTATTTTA TTCTGATTCT GAGGAAAAGG GGAAAATCGG TGCACTTAAT 
GATATGACTC GAAAGGCTGC AATGGAGCAG AAGGCTGATG CTGAAACACT CCGATATAAT 
CTGGCAAAGT CTGGCTTTAG CGTGTTGTTG GCGGAAGACA ACATTATCAA TATTAAGGTT 
ATAAGCCGTT ACCTTGAAAG AATTGGTGTC AAATTCAAGG TCACCATGGA CGGTTTGCAA 
TGTGTTGAAG AATGGAAACG TGAAAAGCCT AATTTTTACT CTCTTATTCT AATGGATTTA 
CAAATGCCTG TTATGGATGG TTACCAGGCA TGTAATGAAA TTCGTAAATA CGAATTGGAA 
AACGATTACC CTAAAGTTCC TATAGTTGCA CTTAGTGCGA ATGCTTTACC TCATGTTGTT 
TTAAGCTGCA AAGATAGTGG TTTTGATTCT TACCTTGCCA AACCTATTAC TTTGCAACAC 
TTGTCCTTAA TCATATCTGG CATACTTAAT TATACGAACC AATCAAAGTT ACACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mak1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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