SPAC227.05 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPAC227.05 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC227.05 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103232 NM_001018359.2
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Schizosaccharomyces pombe putative prefoldin subunit 4 (SPAC227.05), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc103232
    Clone ID Related Accession (Same CDS sequence) NM_001018359.2
    Accession Version NM_001018359.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative prefoldin subunit 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018359.1. COMPLETENESS: incomplete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGGAACAAG TTCCTGTTCT AGCAGAAGAT CAAAGGAATT TAAATGAATT TTCAGTGTTG 
    CATTCTCGAA AGGCCATTCA AGAACTTGAT ATTAAGAATT TAAAAACGCA AATAGAGGAT
    ATTGTTGACG CTAAAAATGA ATGTGAATTA CTAGACGAGG ATGATGGAGA CGATATTCCA
    GCTTTAAAGG TTGGCGATGC CTTTTTCCAA GTTTCCCTTC CTGTACTCCT TGACCAGCTT
    GAGCAGTCAG AAGAAAGTTT GGAAAAGCAA GTCGACGTAT TAAGATCTTC CATGGAAAAA
    GACGAAACCC GCATTCAAGA GTTGAAATCA ATGCTGTACT CTAAATTTCA CGATCAAATT
    AACCTAGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_592959.1
    CDS83..454
    Translation

    Target ORF information:

    RefSeq Version NM_001018359.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative prefoldin subunit 4 (SPAC227.05), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001018359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGAACAAG TTCCTGTTCT AGCAGAAGAT CAAAGGAATT TAAATGAATT TTCAGTGTTG 
    CATTCTCGAA AGGCCATTCA AGAACTTGAT ATTAAGAATT TAAAAACGCA AATAGAGGAT
    ATTGTTGACG CTAAAAATGA ATGTGAATTA CTAGACGAGG ATGATGGAGA CGATATTCCA
    GCTTTAAAGG TTGGCGATGC CTTTTTCCAA GTTTCCCTTC CTGTACTCCT TGACCAGCTT
    GAGCAGTCAG AAGAAAGTTT GGAAAAGCAA GTCGACGTAT TAAGATCTTC CATGGAAAAA
    GACGAAACCC GCATTCAAGA GTTGAAATCA ATGCTGTACT CTAAATTTCA CGATCAAATT
    AACCTAGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L