cdc12 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc12
Entrez Gene ID	2541603
Full Name	formin Cdc12
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018269.2	NP_592869.1	formin Cdc12

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this paper defines a short motif within the N terminus of Cdc12 that binds directly to the F-BAR domain of the scaffolding protein Cdc15.
Title: The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12.

Data indicate that the essential role of profilin is to specifically facilitate formin Cdc12-mediated actin assembly for cytokinesis.
Title: Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12.

Cdc12 and for3 cooperate in cytokinesis and de novo actin assembly at the division site is predominant for contractile ring formation.
Title: The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis.

Cdc12 is an efficient nucleator (one filament per approximately 3 Cdc12 dimers) that processively elongates profilin-actin at a moderate rate of 10 subunits s-1 muM-1, but lacks filament-bundling activity.
Title: The functionally distinct fission yeast formins have specific actin-assembly properties.

cdc12p contributes at least two separable activities: actin assembly and one or more additional functions in cytokinesis initiation
Title: Regulation of cytokinesis by the formin cdc12p.

The following cdc12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103702	NM_001018269.2 Latest version!	Schizosaccharomyces pombe formin Cdc12 (cdc12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc12 ( NM_001018269.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_592869.1 Schizosaccharomyces pombe formin Cdc12 (cdc12), mRNA.

Price & Availability↑

CloneID	OSc103702
Clone ID Related Accession (Same CDS sequence)	NM_001018269.2
Accession Version	NM_001018269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	formin Cdc12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018269.1.

ATGCGAAATT CGTCAAAGGG ACAGGACCCT AATTTTTCTT ATGACTCTAT TCTGTCTACT 
CCAACTCCAT CAGCCAGAAG AACTATTGGG CCAAGGGCTC CTAAGTCCAA AACAACGTAC 
CATAAACCTC CATCATCCAT AGAATCAGTA TCGACGTTAA TCCAGCCTAA TAAGTCCCAA 
TCAGTTACTT CTCCGTATGT AAAGCAATTT ACCTTTTCAT CGAAAGAGTA CAATTCTCAT 
AATAAACATG CTTTGCAAAA TTCCCAGCTT CCTTTACCTA AAACACCCGA GAAATCTACT 
GTCCATCGAC CCAAAGCGAA TAAAGTTGAA GTCACTGATC TACCATCTTC GTCTTCCGTT 
GAACATTTGC ACACTTCTAA ACATCTCAAG GGTCCTAGGT TGCCCAAAAA CATTATTAAA 
AGTTCTGAGG ATGTCCAAAT TGCTCCAGTG ACACCCCCTG TACATTCTCG CTCTTTTGAT 
CCTTTGCCGA AACCTCCTGT TCCCTCAGTT CCAGTATCTA AGACAAAAAG GCGAACTAAG 
CATAAACTTG CTCCTGTTGT TGAAGTCCCT GAGATTACCA ACGAGGTTTC CCCAAAATTC 
ACTTCTACTA ATGATGAACA AGTTTATCGA CTACGTTCAA TTCGAGCTGG GAGCCCTAAT 
TCGGTATGCT CGTTTCAGTT TGAAATTCCG AGTACTCGAC CACCTTCATT GGATCAGCTA 
ATACATCTAT TCAACGATTT TTTAAGACAC CCTGTATTTG ATTTTGACGA AAACGCCATT 
GAAATGCTTC AAAGTTGTAC TCCAGATGAA AAGTGGTGTT TTATTCGTTC TAACTTCGCC 
GGGTTTGATG ATCCTTCTTT CCAAATTCCA GAATTAGCTG CCGTCCATAG ACCTGTTTCC 
TGGTTCGTAA TCCAACTATG GAATAAAACA ATTAGTAATT TACAACTTAT CACACTTTCT 
TCTTTATTAT CAACTCAATC CGATCGATGG ATATCTCTTT TTCTTGAACT GCAGGGACTT 
CGAGCTTTGC ATAATCTACT TACATATTTT AATTCGTCTG CCGTAGTGCA GCCTCAGCAA 
GCTGAAGTTC CTCGTTGCAT GCTTACTCTT TTGAAGAAAA AACCTACTCT GGTTACTTCC 
AATTCTTATA TATTTCAAGC AATAACAGTG ACCCTTATAT CCCCAAATTT GCTTCCGAGG 
AAAGTAGCCG CAGATTTATT GACATGGGTT CTGTCTTTAA AGGAACCTCT TGTGGTCTCC 
ATATTGGAAA CTGGATTTAA AGAAATAAAT GCCGAATATG AAAAGGAGGT TCCGCTATTC 
TTTGGATGGA TAAAATCTTT CAAAGACATA ATCTTGGAAA AAGAATTAGC ACGAACCCCT 
CCATCTTCTC CTGCTCGAAA TTCTGCTTCC AGCTCACCTT CAAACATAGC GTTTCTTGAG 
TATTGTACTT CCACTATGGA ATTTATCAAT CAGCTAATTG TTGCTTGCGA AGAATTGGAG 
CAAGGATTTG ACCTTGATAT ATTGGATTCC CTTCGTGAAT CAGGTATACA CGAAGTTATA 
CAACTTCTAC GTAACTTTCC TGACCAACAA CTTGAGAAAC AACTAAACAT ATATGAATCA 
GAAGAAGAGA GGAGGACTAT AAGTCAGACC ACGCATGAAG ATGTCGATTC CTTTATGTCA 
AATGAATCCT CAATTTTAAG TAGCTTCAAT GAATTTGCAT CAAATGAAGT TGGCCGTTTA 
CTTGAATCAA CTATTCAAAA CATACTACTT GCCAAAGGTA CTGAAAAGCA AAAGGTGAAA 
CTTATCAAGG TTTTTAACTC TTTACTTCAA AGAATACTGC TCAATTCAAA AGTATCCAAT 
GAAAGTTTTG AAGATAGTTT ACAGGCATCT TTAAATATGC TAACCGAAAG ATTCTATTCG 
GATGACACAG CCAGAAATGC TTTGAAGGAA GCAAAGGCGT CACGTGCAAT GGCTGAGAAA 
ATGGTTATTG AAAGAGATGC AATGGCTGCG CAAGTAAATC TGGGTGCCGA AGACTTAATT 
GCGAAGTTAA ACAAAGAAGT AGAGGATCAG AAAGACGTTA TTCTTTCTCA GAAAAGAACG 
AATGAGACTC TAAAAACAGA AATTGATGCT CTCCAAAAGT CTCATGTTAC TCAAATCCAA 
AGGAGCGAAG TGGAATTACG AGAATTGTAT CTTCTTATCA ATTCGGATTC TTTTCAGGGC 
TCAACTAATT CCAAGGAAAG GATTATAGAA TATTTGTTGG ATAAGCTTGA CTTGAGGAAA 
AAGGAAATTG CTGCAGAGTC AACACTTTGG TCAAACGATG GAATTGATGA CAAACTTCGG 
GATCTAAGGG AGCAAATGTC TCGACAGTCC AGCCAGCCCT CAACTGTGTC AACTATTTTA 
CAAATTCCTG ATAAAAAATT TCATCGTCCA TTCCCTCGTC ATTTACATCG ATATGTTGGT 
CGTTCAGCTT CTGAATCGTT AACCAGTGAA AAGGATGAGT CCATCAAAAG TATGAAAGGA 
ATTGATGACT TTGCGAACTT GGAGATTCCG GGAAAGGGTA TTGAGTCAAA CGTCGTAATA 
AAGGATATTT CAAATCAAAC GCATGAGATT AATAGCGTTG AGAACAAAGC AGAAACAGTT 
AGCAACAATA GTAAAATTAC GAACTTTGAT ATTCCTAATG ATGCAACTTC TTTACCAACG 
ATAATTACAC ATCCAACTCC ACCACCACCT CCTCCTCTTC CTGTTAAAAC TAGTTTAAAT 
ACCTTTTCCC ATCCTGATTC TGTGAACATC GTTGCTAATG ATACATCGGT TGCTGGGGTT 
ATGCCTGCTT TTCCACCACC ACCACCACCT CCTCCTCCCT TAGTAAGTGC AGCTGGAGGT 
AAATTCGTAT CCCCAGCTGT TTCAAATAAT ATATCAAAAG ATGATTTGCA TAAGACTACT 
GGTTTGACTC GTCGACCAAC TCGTCGTTTG AAGCAAATGC ATTGGGAGAA GTTGAATTCT 
GGGCTGGAAT TTACATTTTG GACGGGACCT TCTGACGAGG CCAACAAAAT TTTGGAAACC 
TTGCATACAT CAGGGGTCTT AGATGAATTA GATGAAAGTT TTGCGATGAA GGAAGCAAAA 
ACATTGGTCA AAAAAACATG TGCTAGAACT GATTACATGA GTAGTGAACT TCAGAAGCTT 
TTTGGTATTC ACTTTCACAA GCTGTCTCAC AAAAATCCGA ATGAGATCAT TCGCATGATT 
TTACATTGTG ATGATAGCAT GAACGAGTGT GTTGAATTTT TATCAAGTGA CAAAGTACTA 
AATCAACCTA AATTAAAAGC AGATTTGGAG CCTTATCGTA TTGACTGGGC AAATGGTGGT 
GATCTAGTTA ATAGCGAAAA GGATGCTTCA GAGCTTAGTC GTTGGGACTA TCTTTATGTG 
AGACTTATAG TTGATCTTGG GGGTTATTGG AACCAAAGAA TGAATGCACT AAAAGTTAAA 
AACATAATCG AAACCAATTA TGAGAACCTT GTTCGTCAAA CAAAACTTAT AGGGCGGGCT 
GCTCTTGAGC TTCGGGACAG CAAAGTTTTT AAAGGTTTGC TATATTTAAT TCTATATTTA 
GGAAACTATA TGAACGATTA TGTGCGCCAA GCTAAAGGAT TTGCCATTGG TTCACTTCAA 
AGACTTCCAT TGATAAAAAA TGCCAATAAC ACTAAAAGTT TACTTCATAT ACTTGATATA 
ACTATTAGGA AGCATTTTCC TCAGTTTGAC AACTTTTCCC CTGAACTATC AACCGTCACT 
GAAGCAGCCA AACTGAATAT TGAAGCGATT GAACAAGAGT GCTCCGAGTT AATTCGTGGT 
TGTCAGAATT TACAAATAGA CTGTGATTCT GGGGCTCTTT CCGACCCTAC GGTATTTCAT 
CCTGATGATA AAATCCTTTC AGTTATATTA CCCTGGTTAA TGGAAGGCAC CAAAAAAATG 
GATTTTTTGA AAGAGCATTT ACGTACAATG AATACTACCC TGAACAATGC TATGAGATAT 
TTTGGTGAAC AGCCTAATGA CCCTAATTCC AAGAACCTGT TCTTTAAACG TGTTGATTCT 
TTCATAATTG ACTACTCTAA GGCTAGATCT GACAACTTGA AATCGGAAGA AGAAGAAGCT 
TCACAGCATA GACGTTTGAA TCTTGTCAAC AATCATAAAG AACACGTGCT TGAACGTGCA 
ATGTCTGAAA ATAACAAAAT GGATAATGAA GCAATGGATG GTTTCCTTGA TAAATTGAGA 
AACGTCAAAT TAGAAAGTCA CCATAAACCA CGAAATAGAT CCGCCATTAC TATGGGTAAA 
GAACATTTAA TAGAAGCTCC TAACACTTCT ACTAAATCTA GTCCTGCAAA AAACGAATTA 
TTTGTTCCTA AGAGATCTTC GGTAAAAAGT GACTTGGCAA AAGTTAGGCC AAGGTATCCT 
AAGGGTAGTG AATCAACCGA TGGATTATCT GACGCATTAA ATATTACTCC AACGAAAAAA 
GGAGAAGTAT CTAGCAAAGC TAAAAAGGGA TACAACTACG AAAAGCGTCG TTCGGGCCGT 
CAGGTATCTG ATAGCTATGT CCTTAACAAA AATTCGAAGA ATAAAAGTAA TAAGGGTCGC 
TCCGCTTCTT ACACTTTTTC TGATCCAAGC AGTTTGGAAG ATTCTAATAG GCAAAAACCC 
TTTAATGGTG AGAAATTTCG AAGGTTTAGT TCAAAATCAA GAAGAGGTTC ACAAAACCGG 
GATTCTAAGA AGACGGGTAA AGCTCGAAAG GATAAAGGTA TAAACAATAA CCAGACAAGC 
CCTCAGAATA AACCGTCTAA AGAATCTCTA AAATCTGATA CTATTTCAAA CGAGAAGAAA 
GTATTTCCTC AAAAGGCTTC TAAAGTTAAT TTGCTTACGC CGACAATTTC AAATGGTACT 
AGGGCAAGTA AACATGCTAA CGAAAAGGAG AATACTTTTC CTCGTGGTGT CGAAAATAAT 
CTGGTTGCTC CCATGATTCC TAATAACACC GAACTTAATG AAGATACCAG CGCTGTCTCT 
CGGAACTTAG AAAATGCCAC CAATGACTTG AAGGAAACTT TTCCGACTAC AACTACGATT 
TCAACAGCCA GAGCTAAACC TGGTAACAAT GATATTAACA CTATTTTACG AAGAAACAAT 
AGCCGTGGCC GTCGACGCAT GTTGCAGCAA ATGAGTCCTT TGAAAAGCAA TAAATTTTCG 
GGAACTAATG ATTTGAATTT TCAACAAGCT ACTAAACCAG ACGGCAGCAA TAAGTCCTCA 
TATATGGAAC GTCTGGAAAA ATTGAAACAA AACTCTGAAC GACATCTTCA ATCAGTAGGA 
GGTAAGAAGG TTTATTCTTC TGAAGAAACG CCCGTCAACA AAATATTAGT ATCGCCTTCC 
GTTTCTATTT TGGATCATAA CCGAATCTTA AGTCAAAGTA CTCCTATCAA ATCACCACAA 
CGAGCACAAG AAATGCTTGC AGGTTTATTA TCTGGAAAAT TGGCGCCTAA GGAGAATGAG 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_592869.1
CDS	190..5715
Translation	MRNSSKGQDPNFSYDSILSTPTPSARRTIGPRAPKSKTTYHKPPSSIESVSTLIQPNKSQSVTSPYVKQFTFSSKEYNSHNKHALQNSQLPLPKTPEKSTVHRPKANKVEVTDLPSSSSVEHLHTSKHLKGPRLPKNIIKSSEDVQIAPVTPPVHSRSFDPLPKPPVPSVPVSKTKRRTKHKLAPVVEVPEITNEVSPKFTSTNDEQVYRLRSIRAGSPNSVCSFQFEIPSTRPPSLDQLIHLFNDFLRHPVFDFDENAIEMLQSCTPDEKWCFIRSNFAGFDDPSFQIPELAAVHRPVSWFVIQLWNKTISNLQLITLSSLLSTQSDRWISLFLELQGLRALHNLLTYFNSSAVVQPQQAEVPRCMLTLLKKKPTLVTSNSYIFQAITVTLISPNLLPRKVAADLLTWVLSLKEPLVVSILETGFKEINAEYEKEVPLFFGWIKSFKDIILEKELARTPPSSPARNSASSSPSNIAFLEYCTSTMEFINQLIVACEELEQGFDLDILDSLRESGIHEVIQLLRNFPDQQLEKQLNIYESEEERRTISQTTHEDVDSFMSNESSILSSFNEFASNEVGRLLESTIQNILLAKGTEKQKVKLIKVFNSLLQRILLNSKVSNESFEDSLQASLNMLTERFYSDDTARNALKEAKASRAMAEKMVIERDAMAAQVNLGAEDLIAKLNKEVEDQKDVILSQKRTNETLKTEIDALQKSHVTQIQRSEVELRELYLLINSDSFQGSTNSKERIIEYLLDKLDLRKKEIAAESTLWSNDGIDDKLRDLREQMSRQSSQPSTVSTILQIPDKKFHRPFPRHLHRYVGRSASESLTSEKDESIKSMKGIDDFANLEIPGKGIESNVVIKDISNQTHEINSVENKAETVSNNSKITNFDIPNDATSLPTIITHPTPPPPPPLPVKTSLNTFSHPDSVNIVANDTSVAGVMPAFPPPPPPPPPLVSAAGGKFVSPAVSNNISKDDLHKTTGLTRRPTRRLKQMHWEKLNSGLEFTFWTGPSDEANKILETLHTSGVLDELDESFAMKEAKTLVKKTCARTDYMSSELQKLFGIHFHKLSHKNPNEIIRMILHCDDSMNECVEFLSSDKVLNQPKLKADLEPYRIDWANGGDLVNSEKDASELSRWDYLYVRLIVDLGGYWNQRMNALKVKNIIETNYENLVRQTKLIGRAALELRDSKVFKGLLYLILYLGNYMNDYVRQAKGFAIGSLQRLPLIKNANNTKSLLHILDITIRKHFPQFDNFSPELSTVTEAAKLNIEAIEQECSELIRGCQNLQIDCDSGALSDPTVFHPDDKILSVILPWLMEGTKKMDFLKEHLRTMNTTLNNAMRYFGEQPNDPNSKNLFFKRVDSFIIDYSKARSDNLKSEEEEASQHRRLNLVNNHKEHVLERAMSENNKMDNEAMDGFLDKLRNVKLESHHKPRNRSAITMGKEHLIEAPNTSTKSSPAKNELFVPKRSSVKSDLAKVRPRYPKGSESTDGLSDALNITPTKKGEVSSKAKKGYNYEKRRSGRQVSDSYVLNKNSKNKSNKGRSASYTFSDPSSLEDSNRQKPFNGEKFRRFSSKSRRGSQNRDSKKTGKARKDKGINNNQTSPQNKPSKESLKSDTISNEKKVFPQKASKVNLLTPTISNGTRASKHANEKENTFPRGVENNLVAPMIPNNTELNEDTSAVSRNLENATNDLKETFPTTTTISTARAKPGNNDINTILRRNNSRGRRRMLQQMSPLKSNKFSGTNDLNFQQATKPDGSNKSSYMERLEKLKQNSERHLQSVGGKKVYSSEETPVNKILVSPSVSILDHNRILSQSTPIKSPQRAQEMLAGLLSGKLAPKENEK

Target ORF information:

RefSeq Version	NM_001018269.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe formin Cdc12 (cdc12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018269.2

ORF Insert Sequence:

ATGCGAAATT CGTCAAAGGG ACAGGACCCT AATTTTTCTT ATGACTCTAT TCTGTCTACT 
CCAACTCCAT CAGCCAGAAG AACTATTGGG CCAAGGGCTC CTAAGTCCAA AACAACGTAC 
CATAAACCTC CATCATCCAT AGAATCAGTA TCGACGTTAA TCCAGCCTAA TAAGTCCCAA 
TCAGTTACTT CTCCGTATGT AAAGCAATTT ACCTTTTCAT CGAAAGAGTA CAATTCTCAT 
AATAAACATG CTTTGCAAAA TTCCCAGCTT CCTTTACCTA AAACACCCGA GAAATCTACT 
GTCCATCGAC CCAAAGCGAA TAAAGTTGAA GTCACTGATC TACCATCTTC GTCTTCCGTT 
GAACATTTGC ACACTTCTAA ACATCTCAAG GGTCCTAGGT TGCCCAAAAA CATTATTAAA 
AGTTCTGAGG ATGTCCAAAT TGCTCCAGTG ACACCCCCTG TACATTCTCG CTCTTTTGAT 
CCTTTGCCGA AACCTCCTGT TCCCTCAGTT CCAGTATCTA AGACAAAAAG GCGAACTAAG 
CATAAACTTG CTCCTGTTGT TGAAGTCCCT GAGATTACCA ACGAGGTTTC CCCAAAATTC 
ACTTCTACTA ATGATGAACA AGTTTATCGA CTACGTTCAA TTCGAGCTGG GAGCCCTAAT 
TCGGTATGCT CGTTTCAGTT TGAAATTCCG AGTACTCGAC CACCTTCATT GGATCAGCTA 
ATACATCTAT TCAACGATTT TTTAAGACAC CCTGTATTTG ATTTTGACGA AAACGCCATT 
GAAATGCTTC AAAGTTGTAC TCCAGATGAA AAGTGGTGTT TTATTCGTTC TAACTTCGCC 
GGGTTTGATG ATCCTTCTTT CCAAATTCCA GAATTAGCTG CCGTCCATAG ACCTGTTTCC 
TGGTTCGTAA TCCAACTATG GAATAAAACA ATTAGTAATT TACAACTTAT CACACTTTCT 
TCTTTATTAT CAACTCAATC CGATCGATGG ATATCTCTTT TTCTTGAACT GCAGGGACTT 
CGAGCTTTGC ATAATCTACT TACATATTTT AATTCGTCTG CCGTAGTGCA GCCTCAGCAA 
GCTGAAGTTC CTCGTTGCAT GCTTACTCTT TTGAAGAAAA AACCTACTCT GGTTACTTCC 
AATTCTTATA TATTTCAAGC AATAACAGTG ACCCTTATAT CCCCAAATTT GCTTCCGAGG 
AAAGTAGCCG CAGATTTATT GACATGGGTT CTGTCTTTAA AGGAACCTCT TGTGGTCTCC 
ATATTGGAAA CTGGATTTAA AGAAATAAAT GCCGAATATG AAAAGGAGGT TCCGCTATTC 
TTTGGATGGA TAAAATCTTT CAAAGACATA ATCTTGGAAA AAGAATTAGC ACGAACCCCT 
CCATCTTCTC CTGCTCGAAA TTCTGCTTCC AGCTCACCTT CAAACATAGC GTTTCTTGAG 
TATTGTACTT CCACTATGGA ATTTATCAAT CAGCTAATTG TTGCTTGCGA AGAATTGGAG 
CAAGGATTTG ACCTTGATAT ATTGGATTCC CTTCGTGAAT CAGGTATACA CGAAGTTATA 
CAACTTCTAC GTAACTTTCC TGACCAACAA CTTGAGAAAC AACTAAACAT ATATGAATCA 
GAAGAAGAGA GGAGGACTAT AAGTCAGACC ACGCATGAAG ATGTCGATTC CTTTATGTCA 
AATGAATCCT CAATTTTAAG TAGCTTCAAT GAATTTGCAT CAAATGAAGT TGGCCGTTTA 
CTTGAATCAA CTATTCAAAA CATACTACTT GCCAAAGGTA CTGAAAAGCA AAAGGTGAAA 
CTTATCAAGG TTTTTAACTC TTTACTTCAA AGAATACTGC TCAATTCAAA AGTATCCAAT 
GAAAGTTTTG AAGATAGTTT ACAGGCATCT TTAAATATGC TAACCGAAAG ATTCTATTCG 
GATGACACAG CCAGAAATGC TTTGAAGGAA GCAAAGGCGT CACGTGCAAT GGCTGAGAAA 
ATGGTTATTG AAAGAGATGC AATGGCTGCG CAAGTAAATC TGGGTGCCGA AGACTTAATT 
GCGAAGTTAA ACAAAGAAGT AGAGGATCAG AAAGACGTTA TTCTTTCTCA GAAAAGAACG 
AATGAGACTC TAAAAACAGA AATTGATGCT CTCCAAAAGT CTCATGTTAC TCAAATCCAA 
AGGAGCGAAG TGGAATTACG AGAATTGTAT CTTCTTATCA ATTCGGATTC TTTTCAGGGC 
TCAACTAATT CCAAGGAAAG GATTATAGAA TATTTGTTGG ATAAGCTTGA CTTGAGGAAA 
AAGGAAATTG CTGCAGAGTC AACACTTTGG TCAAACGATG GAATTGATGA CAAACTTCGG 
GATCTAAGGG AGCAAATGTC TCGACAGTCC AGCCAGCCCT CAACTGTGTC AACTATTTTA 
CAAATTCCTG ATAAAAAATT TCATCGTCCA TTCCCTCGTC ATTTACATCG ATATGTTGGT 
CGTTCAGCTT CTGAATCGTT AACCAGTGAA AAGGATGAGT CCATCAAAAG TATGAAAGGA 
ATTGATGACT TTGCGAACTT GGAGATTCCG GGAAAGGGTA TTGAGTCAAA CGTCGTAATA 
AAGGATATTT CAAATCAAAC GCATGAGATT AATAGCGTTG AGAACAAAGC AGAAACAGTT 
AGCAACAATA GTAAAATTAC GAACTTTGAT ATTCCTAATG ATGCAACTTC TTTACCAACG 
ATAATTACAC ATCCAACTCC ACCACCACCT CCTCCTCTTC CTGTTAAAAC TAGTTTAAAT 
ACCTTTTCCC ATCCTGATTC TGTGAACATC GTTGCTAATG ATACATCGGT TGCTGGGGTT 
ATGCCTGCTT TTCCACCACC ACCACCACCT CCTCCTCCCT TAGTAAGTGC AGCTGGAGGT 
AAATTCGTAT CCCCAGCTGT TTCAAATAAT ATATCAAAAG ATGATTTGCA TAAGACTACT 
GGTTTGACTC GTCGACCAAC TCGTCGTTTG AAGCAAATGC ATTGGGAGAA GTTGAATTCT 
GGGCTGGAAT TTACATTTTG GACGGGACCT TCTGACGAGG CCAACAAAAT TTTGGAAACC 
TTGCATACAT CAGGGGTCTT AGATGAATTA GATGAAAGTT TTGCGATGAA GGAAGCAAAA 
ACATTGGTCA AAAAAACATG TGCTAGAACT GATTACATGA GTAGTGAACT TCAGAAGCTT 
TTTGGTATTC ACTTTCACAA GCTGTCTCAC AAAAATCCGA ATGAGATCAT TCGCATGATT 
TTACATTGTG ATGATAGCAT GAACGAGTGT GTTGAATTTT TATCAAGTGA CAAAGTACTA 
AATCAACCTA AATTAAAAGC AGATTTGGAG CCTTATCGTA TTGACTGGGC AAATGGTGGT 
GATCTAGTTA ATAGCGAAAA GGATGCTTCA GAGCTTAGTC GTTGGGACTA TCTTTATGTG 
AGACTTATAG TTGATCTTGG GGGTTATTGG AACCAAAGAA TGAATGCACT AAAAGTTAAA 
AACATAATCG AAACCAATTA TGAGAACCTT GTTCGTCAAA CAAAACTTAT AGGGCGGGCT 
GCTCTTGAGC TTCGGGACAG CAAAGTTTTT AAAGGTTTGC TATATTTAAT TCTATATTTA 
GGAAACTATA TGAACGATTA TGTGCGCCAA GCTAAAGGAT TTGCCATTGG TTCACTTCAA 
AGACTTCCAT TGATAAAAAA TGCCAATAAC ACTAAAAGTT TACTTCATAT ACTTGATATA 
ACTATTAGGA AGCATTTTCC TCAGTTTGAC AACTTTTCCC CTGAACTATC AACCGTCACT 
GAAGCAGCCA AACTGAATAT TGAAGCGATT GAACAAGAGT GCTCCGAGTT AATTCGTGGT 
TGTCAGAATT TACAAATAGA CTGTGATTCT GGGGCTCTTT CCGACCCTAC GGTATTTCAT 
CCTGATGATA AAATCCTTTC AGTTATATTA CCCTGGTTAA TGGAAGGCAC CAAAAAAATG 
GATTTTTTGA AAGAGCATTT ACGTACAATG AATACTACCC TGAACAATGC TATGAGATAT 
TTTGGTGAAC AGCCTAATGA CCCTAATTCC AAGAACCTGT TCTTTAAACG TGTTGATTCT 
TTCATAATTG ACTACTCTAA GGCTAGATCT GACAACTTGA AATCGGAAGA AGAAGAAGCT 
TCACAGCATA GACGTTTGAA TCTTGTCAAC AATCATAAAG AACACGTGCT TGAACGTGCA 
ATGTCTGAAA ATAACAAAAT GGATAATGAA GCAATGGATG GTTTCCTTGA TAAATTGAGA 
AACGTCAAAT TAGAAAGTCA CCATAAACCA CGAAATAGAT CCGCCATTAC TATGGGTAAA 
GAACATTTAA TAGAAGCTCC TAACACTTCT ACTAAATCTA GTCCTGCAAA AAACGAATTA 
TTTGTTCCTA AGAGATCTTC GGTAAAAAGT GACTTGGCAA AAGTTAGGCC AAGGTATCCT 
AAGGGTAGTG AATCAACCGA TGGATTATCT GACGCATTAA ATATTACTCC AACGAAAAAA 
GGAGAAGTAT CTAGCAAAGC TAAAAAGGGA TACAACTACG AAAAGCGTCG TTCGGGCCGT 
CAGGTATCTG ATAGCTATGT CCTTAACAAA AATTCGAAGA ATAAAAGTAA TAAGGGTCGC 
TCCGCTTCTT ACACTTTTTC TGATCCAAGC AGTTTGGAAG ATTCTAATAG GCAAAAACCC 
TTTAATGGTG AGAAATTTCG AAGGTTTAGT TCAAAATCAA GAAGAGGTTC ACAAAACCGG 
GATTCTAAGA AGACGGGTAA AGCTCGAAAG GATAAAGGTA TAAACAATAA CCAGACAAGC 
CCTCAGAATA AACCGTCTAA AGAATCTCTA AAATCTGATA CTATTTCAAA CGAGAAGAAA 
GTATTTCCTC AAAAGGCTTC TAAAGTTAAT TTGCTTACGC CGACAATTTC AAATGGTACT 
AGGGCAAGTA AACATGCTAA CGAAAAGGAG AATACTTTTC CTCGTGGTGT CGAAAATAAT 
CTGGTTGCTC CCATGATTCC TAATAACACC GAACTTAATG AAGATACCAG CGCTGTCTCT 
CGGAACTTAG AAAATGCCAC CAATGACTTG AAGGAAACTT TTCCGACTAC AACTACGATT 
TCAACAGCCA GAGCTAAACC TGGTAACAAT GATATTAACA CTATTTTACG AAGAAACAAT 
AGCCGTGGCC GTCGACGCAT GTTGCAGCAA ATGAGTCCTT TGAAAAGCAA TAAATTTTCG 
GGAACTAATG ATTTGAATTT TCAACAAGCT ACTAAACCAG ACGGCAGCAA TAAGTCCTCA 
TATATGGAAC GTCTGGAAAA ATTGAAACAA AACTCTGAAC GACATCTTCA ATCAGTAGGA 
GGTAAGAAGG TTTATTCTTC TGAAGAAACG CCCGTCAACA AAATATTAGT ATCGCCTTCC 
GTTTCTATTT TGGATCATAA CCGAATCTTA AGTCAAAGTA CTCCTATCAA ATCACCACAA 
CGAGCACAAG AAATGCTTGC AGGTTTATTA TCTGGAAAAT TGGCGCCTAA GGAGAATGAG 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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cdc12 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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