ORF » Species Summary » Schizosaccharomyces pombe » SPAC27E2.06c cDNA ORF clone

SPAC27E2.06c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following SPAC27E2.06c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC27E2.06c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102501 NM_001019838.2
Latest version!		 Schizosaccharomyces pombe putative methionine--tRNA (Met) ligase (SPAC27E2.06c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPAC27E2.06c ( NM_001019838.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594407.1 Schizosaccharomyces pombe putative methionine--tRNA (Met) ligase (SPAC27E2.06c), mRNA.
    Price & Availability↑
    CloneID OSc102501
    Clone ID Related Accession (Same CDS sequence) NM_001019838.2
    Accession Version NM_001019838.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative methionine--tRNA (Met) ligase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019838.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTGCGCA AAGGGATTTG TAGGCTAATT CATCAAGTTT CAGAATCTTC TAAAAAACCT 
    TATTTCCTTA CAACACCTAT TTTCTATGTC AATGCTGCAC CTCATCTCGG TCACTTGTAC 
    AGTCTTGTGT TGACGGATGC CATTGCCAGG TTTCAAAATC TTAAACCCGA TGTTTCCGTC 
    ATTTCCAGTA CTGGCACTGA TGAGCATGGT CTAAAAGTAC AGACTGTTGC CCAAACCGAA 
    GGTGTATCTC CTTTACAGCT ATGTGATCGA AACTCAAAAC GCTTTGCTGA TCTCGCTGTC 
    GCAGCAAATA CAAAATTTAC GCATTTTATT CGTACTACCA ACCCTAAACA TCAAGCATCT 
    GTACAGGAGT TTTGGAAAAC AATCCAAAAA GCTGGAATGA TTTCGTTTGA ACGCCATGAA 
    GGATGGTATT GTGTGAGTGA CGAGACGTTT TATCCAGAAT CAGCCATTCA AAAAGTTGTG 
    GATCCTGCTA CAAAGCAGGA GAAAAGAGTC TCAATGGAAA CGGGAAAAGA GGTTCAATGG 
    TCTAGTGAGA TGAATTACCA TTTTCTTCTC TCTAAATTTC AATCGCGGCT AATTGAGCAT 
    TACAATAAAA ACCCTAACTT TGTTCAACCG TCCATTTTCC ATACTCAGGT TCTAGAAGAG 
    CTTAAAACAG GAATCAGCGA TTTGAGCATT TCGCGACCTA AGCAAAGACT CTCTTGGGGA 
    ATTCCGGTTC CTGGTAATTC ACAACAAACT ATTTATGTAT GGTTAGATGC TTTAATAAAT 
    TATATCAGTG TCATTGGGTA TCCATGGTTG AACGAGAAAT CAAGTCTTAG CGCTGGTTGG 
    CCTGCAAATA TGCATGTAAT TGGAAAGGAT ATAATCAGGT TTCATTGCAT CTACTGGCCC 
    GCTTTTCTAA TGGCTGCTGG GCTTCCATTA CCAGAAAAAA TCCTTGTACA TTCGCATTGG 
    ACAATGAACA AAGTTAAAAT GTCCAAATCT TTAGGCAATG TTGTTGATCC TTTTTGGTTA 
    ATTGAAAAAT ACGGAGTTGA CACTATTCGA TATTATTTAT TAAAACGTGG GAGACTAACG 
    TCCGACTCTA ATTTTGATAT TGAAGAGCTA GAAAAAGACG AAGAACACGA CTTAAGAAGA 
    TCTTTAGGTG TGCTACTTTC TCGATTGCAA TCGAAGAAAC TGTTCATTTC CAATGAAATA 
    CAGAAGCAAT GGCATAAAAA GGATGATTTT ACAGAATACG AAGATATTGT ACACGAACTT 
    ATAGAACTTC CTGTTGTTTG CGCGCAAAGC ATAGATGGTG GGTGTGTTTA CGAGGTTATT 
    AATCTTGTTC AGTCTGTTTT AAGAAGAGTC ACTAAGCTTT TTCAATTGAA AGAACCTTGG 
    AAATTATCAG ATGATTCTCA AGAAAAGATT GACACTTTAA TGTTAGTAGC TCACAGCCTA 
    CGCATTAGCG GAATTCTCCT ACAACCTATA ATGCCAACCA AGTCGACTGA GTTATTAGAC 
    CAATTAGGAA TTCCTAAAAA CCAGCGCTCC CTACAGAATG CAACTAACGT TTTTGAACCT 
    ACGGAGTTCA CGTTCCACAG TGGTAATAAT TCTCATTTGT TTGACAAAAG AACTCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594407.1
    CDS654..2273
    Translation

    Target ORF information:

    RefSeq Version NM_001019838.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative methionine--tRNA (Met) ligase (SPAC27E2.06c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001019838.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTGCGCA AAGGGATTTG TAGGCTAATT CATCAAGTTT CAGAATCTTC TAAAAAACCT 
    TATTTCCTTA CAACACCTAT TTTCTATGTC AATGCTGCAC CTCATCTCGG TCACTTGTAC 
    AGTCTTGTGT TGACGGATGC CATTGCCAGG TTTCAAAATC TTAAACCCGA TGTTTCCGTC 
    ATTTCCAGTA CTGGCACTGA TGAGCATGGT CTAAAAGTAC AGACTGTTGC CCAAACCGAA 
    GGTGTATCTC CTTTACAGCT ATGTGATCGA AACTCAAAAC GCTTTGCTGA TCTCGCTGTC 
    GCAGCAAATA CAAAATTTAC GCATTTTATT CGTACTACCA ACCCTAAACA TCAAGCATCT 
    GTACAGGAGT TTTGGAAAAC AATCCAAAAA GCTGGAATGA TTTCGTTTGA ACGCCATGAA 
    GGATGGTATT GTGTGAGTGA CGAGACGTTT TATCCAGAAT CAGCCATTCA AAAAGTTGTG 
    GATCCTGCTA CAAAGCAGGA GAAAAGAGTC TCAATGGAAA CGGGAAAAGA GGTTCAATGG 
    TCTAGTGAGA TGAATTACCA TTTTCTTCTC TCTAAATTTC AATCGCGGCT AATTGAGCAT 
    TACAATAAAA ACCCTAACTT TGTTCAACCG TCCATTTTCC ATACTCAGGT TCTAGAAGAG 
    CTTAAAACAG GAATCAGCGA TTTGAGCATT TCGCGACCTA AGCAAAGACT CTCTTGGGGA 
    ATTCCGGTTC CTGGTAATTC ACAACAAACT ATTTATGTAT GGTTAGATGC TTTAATAAAT 
    TATATCAGTG TCATTGGGTA TCCATGGTTG AACGAGAAAT CAAGTCTTAG CGCTGGTTGG 
    CCTGCAAATA TGCATGTAAT TGGAAAGGAT ATAATCAGGT TTCATTGCAT CTACTGGCCC 
    GCTTTTCTAA TGGCTGCTGG GCTTCCATTA CCAGAAAAAA TCCTTGTACA TTCGCATTGG 
    ACAATGAACA AAGTTAAAAT GTCCAAATCT TTAGGCAATG TTGTTGATCC TTTTTGGTTA 
    ATTGAAAAAT ACGGAGTTGA CACTATTCGA TATTATTTAT TAAAACGTGG GAGACTAACG 
    TCCGACTCTA ATTTTGATAT TGAAGAGCTA GAAAAAGACG AAGAACACGA CTTAAGAAGA 
    TCTTTAGGTG TGCTACTTTC TCGATTGCAA TCGAAGAAAC TGTTCATTTC CAATGAAATA 
    CAGAAGCAAT GGCATAAAAA GGATGATTTT ACAGAATACG AAGATATTGT ACACGAACTT 
    ATAGAACTTC CTGTTGTTTG CGCGCAAAGC ATAGATGGTG GGTGTGTTTA CGAGGTTATT 
    AATCTTGTTC AGTCTGTTTT AAGAAGAGTC ACTAAGCTTT TTCAATTGAA AGAACCTTGG 
    AAATTATCAG ATGATTCTCA AGAAAAGATT GACACTTTAA TGTTAGTAGC TCACAGCCTA 
    CGCATTAGCG GAATTCTCCT ACAACCTATA ATGCCAACCA AGTCGACTGA GTTATTAGAC 
    CAATTAGGAA TTCCTAAAAA CCAGCGCTCC CTACAGAATG CAACTAACGT TTTTGAACCT 
    ACGGAGTTCA CGTTCCACAG TGGTAATAAT TCTCATTTGT TTGACAAAAG AACTCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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