MetRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MetRS-m
Entrez Gene ID	41733
Full Name	Methionyl-tRNA synthetase, mitochondrial
Synonyms	Aats-met,Aats-met-m,CG31322,CG8684,CG9612,Dmel\CG31322,MRS,MetRS
General protein information	Preferred Names Methionyl-tRNA synthetase, mitochondrial Names CG31322 gene product from transcript CG31322-RC CG31322-PA CG31322-PB CG31322-PC MetRS-m-PA MetRS-m-PB MetRS-m-PC Methionyl-tRNA synthetase mitochondrial methionyl-tRNA synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88B1-88B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_142091.5	NP_650348.1	Methionyl-tRNA synthetase, mitochondrial, isoform A
NM_001316578.1	NP_001303507.1	Methionyl-tRNA synthetase, mitochondrial, isoform C
NM_001275635.2	NP_001262564.1	Methionyl-tRNA synthetase, mitochondrial, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MARS2	XP_516010.3
	MARS2	XP_001086799.2
Bos taurus (cattle)	MARS2	NP_001092441.1
Rattus norvegicus (Norway rat)	LOC100911305	XP_003750747.1
Rattus norvegicus (Norway rat)	Mars2	XP_003750749.1
Danio rerio (zebrafish)	LOC566565	XP_694935.2
Caenorhabditis elegans (roundworm)	CELE_Y105E8A.20	NP_001129797.1
Kluyveromyces lactis	KLLA0F14971g	XP_455753.1
Eremothecium gossypii	AGOS_AAL133W	NP_982409.1
Oryza sativa (rice)	Os03g0209600	NP_001049336.1
Schizosaccharomyces pombe (fission yeast)	SPAC27E2.06c	NP_594407.1
Canis lupus familiaris (dog)	MARS2	XP_005640557.1
Gallus gallus (chicken)	MARS2	XP_426247.3
Neurospora crassa	NCU03759	XP_961106.1
Arabidopsis thaliana (thale cress)	OVA1	NP_191100.1
Xenopus tropicalis (tropical clawed frog)	LOC100488527	XP_002940449.2
Homo sapiens (human)	MARS2	NP_612404.1
Mus musculus (house mouse)	Mars2	NP_780648.1
Drosophila melanogaster (fruit fly)	Aats-met	NP_650348.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002383	XP_312573.4
Saccharomyces cerevisiae (baker's yeast)	MSM1	NP_011687.1
Magnaporthe oryzae (rice blast fungus)	MGG_16221	XP_003709691.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of Aats-met in the eye results in retinal degeneration.
Title: Mutations in the mitochondrial methionyl-tRNA synthetase cause a neurodegenerative phenotype in flies and a recessive ataxia (ARSAL) in humans.

The following MetRS-m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MetRS-m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04848	NM_001316578.1 Latest version!	Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa04848	NM_001275635.2 Latest version!	Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa04848	NM_142091.5 Latest version!	Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MetRS-m ( NM_142091.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650348.1 Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04848
Clone ID Related Accession (Same CDS sequence)	NM_142091.5 , NM_001316578.1 , NM_001275635.2
Accession Version	NM_142091.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methionyl-tRNA synthetase, mitochondrial, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142091.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTATTA GAAGAATTAA GTGCCTCAGA TATTTGGGAA CTCGCTACAA CAGTTCGCAC 
TATGTGACAA CTCCAATTTT CTATGTAAAT GCAGCTCCTC ACATTGGACA CCTGTACTCA 
GCGGTCATAG CCGATGCCCA CTGTCGCTAC CAGAGGCTAA GGTATCCAGA GCAGGATGTA 
CGACTCTGCA CGGGAACGGA TGAGCACGGC ACAAAGATCC AGCAGGCGGC ATCTCTTCAC 
GGGGTGCCCG TTGCCAAATA CTGTGATGAT ATATCGCAGC GATATCGAGA GGTCTTCCGC 
AGTGCCAGCA TCCAGCAGGA TGACTTTATT CGCACCACGG AGGATCGCCA CAAGCGGGCA 
GTGGCCAATT TCTGGCGCAC ACTTCACACA CGCGGCCACA TATATTCGGC CGCCTACAGT 
GGTTGGTATT GCGTGTCTGA CGAAACCTTC CTCACCGACT CCCAGCTGAG ATTGGACGAG 
GCCACTGGTA CCCGATACTC CCTGGAATCT GGACATCCCG TCGAGTGGAC CGAGGAAACG 
AACTATATGT TTCGACTGTC ACAATTCCAG GACGATGTGC GCCATTGGGT GAAAACGGAG 
GCGCGCGTAC GTCCGGCCAA GTTCGAAAAG ATTCTGCTGG ATACGCTCAG CGAACCACTG 
CCGGATGTAT CGGTATCACG TCCTTCCAAC CGCGTACACT GGGCCATTCC CGTTCCCGAT 
GATGACTCGC AGACTGTCTA CGTCTGGTTA GATGCTTTAG TCAACTATCT CAGCTCTGTG 
GGATATCCAG ATGAAAAGTT TTCTGCCCAC TGGCCACCGG CGCAGCAAGT GATAGGGAAG 
GACATCCTCA AGTTCCATGG CATTTACTGG ACAGCTTTCC TCCTGGCTGC CGGCTTGGAA 
CCTCCTGGAC AGCTGTACGT TCACTCGCAT TGGACCGTCG ATGGGCAGAA GATGTCAAAG 
AGCAAGCACA ACGTGGTGGA TCCCCTCCAG GCAGCGCAGC AGTACACCAT GGAGGGCCTG 
CGATACTTCC TTCTGCGGGA GGGCGTGGCC CACAGCGATG GCAACTACAG CCACGTAAAG 
GCGCAGCGCA TCCTCAACTC GGAGCTGGCG GACACCCTGG GCAATCTGCT ATCGCGTGCC 
AGTGCCAAGT CGCTGAATCC CGGCCAAATC TATCCATCAC CCAGCGCGGA GCACTTGGCG 
GATCTCCTCC GGAGCCTGGA TGTGGCAAAA CGCCTGCAGG ATTCGCTTCT GCAGCTGTCA 
GAGCGATGCG AGTCGCATTA TGAGTGCAAT CACTTCCACT TGGTGGCCGA CACAACGATG 
GCGGCTCTGC ATGCGGCCAA CAACTTCTTC GAGAGCTCCA AGCCCTGGAC GCTAAAGGCG 
GGAGCACCGG ATGGCAACCA GGCCCGTTTG GAGACCATAA TCGCCATGAC AATGGACGCG 
TTGCGGCTCA GCGGCATTGT GTTGCAGCCC ATAATTCCCC AGCTGGCCAA CCGGCTGCTC 
GATAAGCTGA GCGTCCCCAC CGCGCAACGT GGCTGGAATT ACTTGGCCGA GAGTTTTGCC 
ACCAGCCCCA ACAGCTCCAA CAGTCCTGCG GGACTCGGCG AGAGCCGTCA ATTGGACGGG 
CAAACCAGTG CGCTGCTCTT CCAGCGGATT CTCGAAGAGA CGTCTGCGAA GGAAGTGAAG 
GAGCAGAAGC CGCAGCCAGC CAAGCGGAGC AAATCGAAGA AGAAGGAGCG CCGCGAAACA 
ATGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650348.1
CDS	85..1833
Translation	MLIRRIKCLRYLGTRYNSSHYVTTPIFYVNAAPHIGHLYSAVIADAHCRYQRLRYPEQDVRLCTGTDEHGTKIQQAASLHGVPVAKYCDDISQRYREVFRSASIQQDDFIRTTEDRHKRAVANFWRTLHTRGHIYSAAYSGWYCVSDETFLTDSQLRLDEATGTRYSLESGHPVEWTEETNYMFRLSQFQDDVRHWVKTEARVRPAKFEKILLDTLSEPLPDVSVSRPSNRVHWAIPVPDDDSQTVYVWLDALVNYLSSVGYPDEKFSAHWPPAQQVIGKDILKFHGIYWTAFLLAAGLEPPGQLYVHSHWTVDGQKMSKSKHNVVDPLQAAQQYTMEGLRYFLLREGVAHSDGNYSHVKAQRILNSELADTLGNLLSRASAKSLNPGQIYPSPSAEHLADLLRSLDVAKRLQDSLLQLSERCESHYECNHFHLVADTTMAALHAANNFFESSKPWTLKAGAPDGNQARLETIIAMTMDALRLSGIVLQPIIPQLANRLLDKLSVPTAQRGWNYLAESFATSPNSSNSPAGLGESRQLDGQTSALLFQRILEETSAKEVKEQKPQPAKRSKSKKKERRETMS

Target ORF information:

RefSeq Version	NM_142091.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142091.5

ORF Insert Sequence:

ATGCTTATTA GAAGAATTAA GTGCCTCAGA TATTTGGGAA CTCGCTACAA CAGTTCGCAC 
TATGTGACAA CTCCAATTTT CTATGTAAAT GCAGCTCCTC ACATTGGACA CCTGTACTCA 
GCGGTCATAG CCGATGCCCA CTGTCGCTAC CAGAGGCTAA GGTATCCAGA GCAGGATGTA 
CGACTCTGCA CGGGAACGGA TGAGCACGGC ACAAAGATCC AGCAGGCGGC ATCTCTTCAC 
GGGGTGCCCG TTGCCAAATA CTGTGATGAT ATATCGCAGC GATATCGAGA GGTCTTCCGC 
AGTGCCAGCA TCCAGCAGGA TGACTTTATT CGCACCACGG AGGATCGCCA CAAGCGGGCA 
GTGGCCAATT TCTGGCGCAC ACTTCACACA CGCGGCCACA TATATTCGGC CGCCTACAGT 
GGTTGGTATT GCGTGTCTGA CGAAACCTTC CTCACCGACT CCCAGCTGAG ATTGGACGAG 
GCCACTGGTA CCCGATACTC CCTGGAATCT GGACATCCCG TCGAGTGGAC CGAGGAAACG 
AACTATATGT TTCGACTGTC ACAATTCCAG GACGATGTGC GCCATTGGGT GAAAACGGAG 
GCGCGCGTAC GTCCGGCCAA GTTCGAAAAG ATTCTGCTGG ATACGCTCAG CGAACCACTG 
CCGGATGTAT CGGTATCACG TCCTTCCAAC CGCGTACACT GGGCCATTCC CGTTCCCGAT 
GATGACTCGC AGACTGTCTA CGTCTGGTTA GATGCTTTAG TCAACTATCT CAGCTCTGTG 
GGATATCCAG ATGAAAAGTT TTCTGCCCAC TGGCCACCGG CGCAGCAAGT GATAGGGAAG 
GACATCCTCA AGTTCCATGG CATTTACTGG ACAGCTTTCC TCCTGGCTGC CGGCTTGGAA 
CCTCCTGGAC AGCTGTACGT TCACTCGCAT TGGACCGTCG ATGGGCAGAA GATGTCAAAG 
AGCAAGCACA ACGTGGTGGA TCCCCTCCAG GCAGCGCAGC AGTACACCAT GGAGGGCCTG 
CGATACTTCC TTCTGCGGGA GGGCGTGGCC CACAGCGATG GCAACTACAG CCACGTAAAG 
GCGCAGCGCA TCCTCAACTC GGAGCTGGCG GACACCCTGG GCAATCTGCT ATCGCGTGCC 
AGTGCCAAGT CGCTGAATCC CGGCCAAATC TATCCATCAC CCAGCGCGGA GCACTTGGCG 
GATCTCCTCC GGAGCCTGGA TGTGGCAAAA CGCCTGCAGG ATTCGCTTCT GCAGCTGTCA 
GAGCGATGCG AGTCGCATTA TGAGTGCAAT CACTTCCACT TGGTGGCCGA CACAACGATG 
GCGGCTCTGC ATGCGGCCAA CAACTTCTTC GAGAGCTCCA AGCCCTGGAC GCTAAAGGCG 
GGAGCACCGG ATGGCAACCA GGCCCGTTTG GAGACCATAA TCGCCATGAC AATGGACGCG 
TTGCGGCTCA GCGGCATTGT GTTGCAGCCC ATAATTCCCC AGCTGGCCAA CCGGCTGCTC 
GATAAGCTGA GCGTCCCCAC CGCGCAACGT GGCTGGAATT ACTTGGCCGA GAGTTTTGCC 
ACCAGCCCCA ACAGCTCCAA CAGTCCTGCG GGACTCGGCG AGAGCCGTCA ATTGGACGGG 
CAAACCAGTG CGCTGCTCTT CCAGCGGATT CTCGAAGAGA CGTCTGCGAA GGAAGTGAAG 
GAGCAGAAGC CGCAGCCAGC CAAGCGGAGC AAATCGAAGA AGAAGGAGCG CCGCGAAACA 
ATGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MetRS-m ( NM_001316578.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303507.1 Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04848
Clone ID Related Accession (Same CDS sequence)	NM_142091.5 , NM_001316578.1 , NM_001275635.2
Accession Version	NM_001316578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methionyl-tRNA synthetase, mitochondrial, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTATTA GAAGAATTAA GTGCCTCAGA TATTTGGGAA CTCGCTACAA CAGTTCGCAC 
TATGTGACAA CTCCAATTTT CTATGTAAAT GCAGCTCCTC ACATTGGACA CCTGTACTCA 
GCGGTCATAG CCGATGCCCA CTGTCGCTAC CAGAGGCTAA GGTATCCAGA GCAGGATGTA 
CGACTCTGCA CGGGAACGGA TGAGCACGGC ACAAAGATCC AGCAGGCGGC ATCTCTTCAC 
GGGGTGCCCG TTGCCAAATA CTGTGATGAT ATATCGCAGC GATATCGAGA GGTCTTCCGC 
AGTGCCAGCA TCCAGCAGGA TGACTTTATT CGCACCACGG AGGATCGCCA CAAGCGGGCA 
GTGGCCAATT TCTGGCGCAC ACTTCACACA CGCGGCCACA TATATTCGGC CGCCTACAGT 
GGTTGGTATT GCGTGTCTGA CGAAACCTTC CTCACCGACT CCCAGCTGAG ATTGGACGAG 
GCCACTGGTA CCCGATACTC CCTGGAATCT GGACATCCCG TCGAGTGGAC CGAGGAAACG 
AACTATATGT TTCGACTGTC ACAATTCCAG GACGATGTGC GCCATTGGGT GAAAACGGAG 
GCGCGCGTAC GTCCGGCCAA GTTCGAAAAG ATTCTGCTGG ATACGCTCAG CGAACCACTG 
CCGGATGTAT CGGTATCACG TCCTTCCAAC CGCGTACACT GGGCCATTCC CGTTCCCGAT 
GATGACTCGC AGACTGTCTA CGTCTGGTTA GATGCTTTAG TCAACTATCT CAGCTCTGTG 
GGATATCCAG ATGAAAAGTT TTCTGCCCAC TGGCCACCGG CGCAGCAAGT GATAGGGAAG 
GACATCCTCA AGTTCCATGG CATTTACTGG ACAGCTTTCC TCCTGGCTGC CGGCTTGGAA 
CCTCCTGGAC AGCTGTACGT TCACTCGCAT TGGACCGTCG ATGGGCAGAA GATGTCAAAG 
AGCAAGCACA ACGTGGTGGA TCCCCTCCAG GCAGCGCAGC AGTACACCAT GGAGGGCCTG 
CGATACTTCC TTCTGCGGGA GGGCGTGGCC CACAGCGATG GCAACTACAG CCACGTAAAG 
GCGCAGCGCA TCCTCAACTC GGAGCTGGCG GACACCCTGG GCAATCTGCT ATCGCGTGCC 
AGTGCCAAGT CGCTGAATCC CGGCCAAATC TATCCATCAC CCAGCGCGGA GCACTTGGCG 
GATCTCCTCC GGAGCCTGGA TGTGGCAAAA CGCCTGCAGG ATTCGCTTCT GCAGCTGTCA 
GAGCGATGCG AGTCGCATTA TGAGTGCAAT CACTTCCACT TGGTGGCCGA CACAACGATG 
GCGGCTCTGC ATGCGGCCAA CAACTTCTTC GAGAGCTCCA AGCCCTGGAC GCTAAAGGCG 
GGAGCACCGG ATGGCAACCA GGCCCGTTTG GAGACCATAA TCGCCATGAC AATGGACGCG 
TTGCGGCTCA GCGGCATTGT GTTGCAGCCC ATAATTCCCC AGCTGGCCAA CCGGCTGCTC 
GATAAGCTGA GCGTCCCCAC CGCGCAACGT GGCTGGAATT ACTTGGCCGA GAGTTTTGCC 
ACCAGCCCCA ACAGCTCCAA CAGTCCTGCG GGACTCGGCG AGAGCCGTCA ATTGGACGGG 
CAAACCAGTG CGCTGCTCTT CCAGCGGATT CTCGAAGAGA CGTCTGCGAA GGAAGTGAAG 
GAGCAGAAGC CGCAGCCAGC CAAGCGGAGC AAATCGAAGA AGAAGGAGCG CCGCGAAACA 
ATGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303507.1
CDS	85..1833
Translation	MLIRRIKCLRYLGTRYNSSHYVTTPIFYVNAAPHIGHLYSAVIADAHCRYQRLRYPEQDVRLCTGTDEHGTKIQQAASLHGVPVAKYCDDISQRYREVFRSASIQQDDFIRTTEDRHKRAVANFWRTLHTRGHIYSAAYSGWYCVSDETFLTDSQLRLDEATGTRYSLESGHPVEWTEETNYMFRLSQFQDDVRHWVKTEARVRPAKFEKILLDTLSEPLPDVSVSRPSNRVHWAIPVPDDDSQTVYVWLDALVNYLSSVGYPDEKFSAHWPPAQQVIGKDILKFHGIYWTAFLLAAGLEPPGQLYVHSHWTVDGQKMSKSKHNVVDPLQAAQQYTMEGLRYFLLREGVAHSDGNYSHVKAQRILNSELADTLGNLLSRASAKSLNPGQIYPSPSAEHLADLLRSLDVAKRLQDSLLQLSERCESHYECNHFHLVADTTMAALHAANNFFESSKPWTLKAGAPDGNQARLETIIAMTMDALRLSGIVLQPIIPQLANRLLDKLSVPTAQRGWNYLAESFATSPNSSNSPAGLGESRQLDGQTSALLFQRILEETSAKEVKEQKPQPAKRSKSKKKERRETMS

Target ORF information:

RefSeq Version	NM_001316578.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316578.1

ORF Insert Sequence:

ATGCTTATTA GAAGAATTAA GTGCCTCAGA TATTTGGGAA CTCGCTACAA CAGTTCGCAC 
TATGTGACAA CTCCAATTTT CTATGTAAAT GCAGCTCCTC ACATTGGACA CCTGTACTCA 
GCGGTCATAG CCGATGCCCA CTGTCGCTAC CAGAGGCTAA GGTATCCAGA GCAGGATGTA 
CGACTCTGCA CGGGAACGGA TGAGCACGGC ACAAAGATCC AGCAGGCGGC ATCTCTTCAC 
GGGGTGCCCG TTGCCAAATA CTGTGATGAT ATATCGCAGC GATATCGAGA GGTCTTCCGC 
AGTGCCAGCA TCCAGCAGGA TGACTTTATT CGCACCACGG AGGATCGCCA CAAGCGGGCA 
GTGGCCAATT TCTGGCGCAC ACTTCACACA CGCGGCCACA TATATTCGGC CGCCTACAGT 
GGTTGGTATT GCGTGTCTGA CGAAACCTTC CTCACCGACT CCCAGCTGAG ATTGGACGAG 
GCCACTGGTA CCCGATACTC CCTGGAATCT GGACATCCCG TCGAGTGGAC CGAGGAAACG 
AACTATATGT TTCGACTGTC ACAATTCCAG GACGATGTGC GCCATTGGGT GAAAACGGAG 
GCGCGCGTAC GTCCGGCCAA GTTCGAAAAG ATTCTGCTGG ATACGCTCAG CGAACCACTG 
CCGGATGTAT CGGTATCACG TCCTTCCAAC CGCGTACACT GGGCCATTCC CGTTCCCGAT 
GATGACTCGC AGACTGTCTA CGTCTGGTTA GATGCTTTAG TCAACTATCT CAGCTCTGTG 
GGATATCCAG ATGAAAAGTT TTCTGCCCAC TGGCCACCGG CGCAGCAAGT GATAGGGAAG 
GACATCCTCA AGTTCCATGG CATTTACTGG ACAGCTTTCC TCCTGGCTGC CGGCTTGGAA 
CCTCCTGGAC AGCTGTACGT TCACTCGCAT TGGACCGTCG ATGGGCAGAA GATGTCAAAG 
AGCAAGCACA ACGTGGTGGA TCCCCTCCAG GCAGCGCAGC AGTACACCAT GGAGGGCCTG 
CGATACTTCC TTCTGCGGGA GGGCGTGGCC CACAGCGATG GCAACTACAG CCACGTAAAG 
GCGCAGCGCA TCCTCAACTC GGAGCTGGCG GACACCCTGG GCAATCTGCT ATCGCGTGCC 
AGTGCCAAGT CGCTGAATCC CGGCCAAATC TATCCATCAC CCAGCGCGGA GCACTTGGCG 
GATCTCCTCC GGAGCCTGGA TGTGGCAAAA CGCCTGCAGG ATTCGCTTCT GCAGCTGTCA 
GAGCGATGCG AGTCGCATTA TGAGTGCAAT CACTTCCACT TGGTGGCCGA CACAACGATG 
GCGGCTCTGC ATGCGGCCAA CAACTTCTTC GAGAGCTCCA AGCCCTGGAC GCTAAAGGCG 
GGAGCACCGG ATGGCAACCA GGCCCGTTTG GAGACCATAA TCGCCATGAC AATGGACGCG 
TTGCGGCTCA GCGGCATTGT GTTGCAGCCC ATAATTCCCC AGCTGGCCAA CCGGCTGCTC 
GATAAGCTGA GCGTCCCCAC CGCGCAACGT GGCTGGAATT ACTTGGCCGA GAGTTTTGCC 
ACCAGCCCCA ACAGCTCCAA CAGTCCTGCG GGACTCGGCG AGAGCCGTCA ATTGGACGGG 
CAAACCAGTG CGCTGCTCTT CCAGCGGATT CTCGAAGAGA CGTCTGCGAA GGAAGTGAAG 
GAGCAGAAGC CGCAGCCAGC CAAGCGGAGC AAATCGAAGA AGAAGGAGCG CCGCGAAACA 
ATGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MetRS-m ( NM_001275635.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262564.1 Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04848
Clone ID Related Accession (Same CDS sequence)	NM_142091.5 , NM_001316578.1 , NM_001275635.2
Accession Version	NM_001275635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methionyl-tRNA synthetase, mitochondrial, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_001275635.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTATTA GAAGAATTAA GTGCCTCAGA TATTTGGGAA CTCGCTACAA CAGTTCGCAC 
TATGTGACAA CTCCAATTTT CTATGTAAAT GCAGCTCCTC ACATTGGACA CCTGTACTCA 
GCGGTCATAG CCGATGCCCA CTGTCGCTAC CAGAGGCTAA GGTATCCAGA GCAGGATGTA 
CGACTCTGCA CGGGAACGGA TGAGCACGGC ACAAAGATCC AGCAGGCGGC ATCTCTTCAC 
GGGGTGCCCG TTGCCAAATA CTGTGATGAT ATATCGCAGC GATATCGAGA GGTCTTCCGC 
AGTGCCAGCA TCCAGCAGGA TGACTTTATT CGCACCACGG AGGATCGCCA CAAGCGGGCA 
GTGGCCAATT TCTGGCGCAC ACTTCACACA CGCGGCCACA TATATTCGGC CGCCTACAGT 
GGTTGGTATT GCGTGTCTGA CGAAACCTTC CTCACCGACT CCCAGCTGAG ATTGGACGAG 
GCCACTGGTA CCCGATACTC CCTGGAATCT GGACATCCCG TCGAGTGGAC CGAGGAAACG 
AACTATATGT TTCGACTGTC ACAATTCCAG GACGATGTGC GCCATTGGGT GAAAACGGAG 
GCGCGCGTAC GTCCGGCCAA GTTCGAAAAG ATTCTGCTGG ATACGCTCAG CGAACCACTG 
CCGGATGTAT CGGTATCACG TCCTTCCAAC CGCGTACACT GGGCCATTCC CGTTCCCGAT 
GATGACTCGC AGACTGTCTA CGTCTGGTTA GATGCTTTAG TCAACTATCT CAGCTCTGTG 
GGATATCCAG ATGAAAAGTT TTCTGCCCAC TGGCCACCGG CGCAGCAAGT GATAGGGAAG 
GACATCCTCA AGTTCCATGG CATTTACTGG ACAGCTTTCC TCCTGGCTGC CGGCTTGGAA 
CCTCCTGGAC AGCTGTACGT TCACTCGCAT TGGACCGTCG ATGGGCAGAA GATGTCAAAG 
AGCAAGCACA ACGTGGTGGA TCCCCTCCAG GCAGCGCAGC AGTACACCAT GGAGGGCCTG 
CGATACTTCC TTCTGCGGGA GGGCGTGGCC CACAGCGATG GCAACTACAG CCACGTAAAG 
GCGCAGCGCA TCCTCAACTC GGAGCTGGCG GACACCCTGG GCAATCTGCT ATCGCGTGCC 
AGTGCCAAGT CGCTGAATCC CGGCCAAATC TATCCATCAC CCAGCGCGGA GCACTTGGCG 
GATCTCCTCC GGAGCCTGGA TGTGGCAAAA CGCCTGCAGG ATTCGCTTCT GCAGCTGTCA 
GAGCGATGCG AGTCGCATTA TGAGTGCAAT CACTTCCACT TGGTGGCCGA CACAACGATG 
GCGGCTCTGC ATGCGGCCAA CAACTTCTTC GAGAGCTCCA AGCCCTGGAC GCTAAAGGCG 
GGAGCACCGG ATGGCAACCA GGCCCGTTTG GAGACCATAA TCGCCATGAC AATGGACGCG 
TTGCGGCTCA GCGGCATTGT GTTGCAGCCC ATAATTCCCC AGCTGGCCAA CCGGCTGCTC 
GATAAGCTGA GCGTCCCCAC CGCGCAACGT GGCTGGAATT ACTTGGCCGA GAGTTTTGCC 
ACCAGCCCCA ACAGCTCCAA CAGTCCTGCG GGACTCGGCG AGAGCCGTCA ATTGGACGGG 
CAAACCAGTG CGCTGCTCTT CCAGCGGATT CTCGAAGAGA CGTCTGCGAA GGAAGTGAAG 
GAGCAGAAGC CGCAGCCAGC CAAGCGGAGC AAATCGAAGA AGAAGGAGCG CCGCGAAACA 
ATGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262564.1
CDS	85..1833
Translation	MLIRRIKCLRYLGTRYNSSHYVTTPIFYVNAAPHIGHLYSAVIADAHCRYQRLRYPEQDVRLCTGTDEHGTKIQQAASLHGVPVAKYCDDISQRYREVFRSASIQQDDFIRTTEDRHKRAVANFWRTLHTRGHIYSAAYSGWYCVSDETFLTDSQLRLDEATGTRYSLESGHPVEWTEETNYMFRLSQFQDDVRHWVKTEARVRPAKFEKILLDTLSEPLPDVSVSRPSNRVHWAIPVPDDDSQTVYVWLDALVNYLSSVGYPDEKFSAHWPPAQQVIGKDILKFHGIYWTAFLLAAGLEPPGQLYVHSHWTVDGQKMSKSKHNVVDPLQAAQQYTMEGLRYFLLREGVAHSDGNYSHVKAQRILNSELADTLGNLLSRASAKSLNPGQIYPSPSAEHLADLLRSLDVAKRLQDSLLQLSERCESHYECNHFHLVADTTMAALHAANNFFESSKPWTLKAGAPDGNQARLETIIAMTMDALRLSGIVLQPIIPQLANRLLDKLSVPTAQRGWNYLAESFATSPNSSNSPAGLGESRQLDGQTSALLFQRILEETSAKEVKEQKPQPAKRSKSKKKERRETMS

Target ORF information:

RefSeq Version	NM_001275635.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methionyl-tRNA synthetase, mitochondrial (MetRS-m), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275635.2

ORF Insert Sequence:

ATGCTTATTA GAAGAATTAA GTGCCTCAGA TATTTGGGAA CTCGCTACAA CAGTTCGCAC 
TATGTGACAA CTCCAATTTT CTATGTAAAT GCAGCTCCTC ACATTGGACA CCTGTACTCA 
GCGGTCATAG CCGATGCCCA CTGTCGCTAC CAGAGGCTAA GGTATCCAGA GCAGGATGTA 
CGACTCTGCA CGGGAACGGA TGAGCACGGC ACAAAGATCC AGCAGGCGGC ATCTCTTCAC 
GGGGTGCCCG TTGCCAAATA CTGTGATGAT ATATCGCAGC GATATCGAGA GGTCTTCCGC 
AGTGCCAGCA TCCAGCAGGA TGACTTTATT CGCACCACGG AGGATCGCCA CAAGCGGGCA 
GTGGCCAATT TCTGGCGCAC ACTTCACACA CGCGGCCACA TATATTCGGC CGCCTACAGT 
GGTTGGTATT GCGTGTCTGA CGAAACCTTC CTCACCGACT CCCAGCTGAG ATTGGACGAG 
GCCACTGGTA CCCGATACTC CCTGGAATCT GGACATCCCG TCGAGTGGAC CGAGGAAACG 
AACTATATGT TTCGACTGTC ACAATTCCAG GACGATGTGC GCCATTGGGT GAAAACGGAG 
GCGCGCGTAC GTCCGGCCAA GTTCGAAAAG ATTCTGCTGG ATACGCTCAG CGAACCACTG 
CCGGATGTAT CGGTATCACG TCCTTCCAAC CGCGTACACT GGGCCATTCC CGTTCCCGAT 
GATGACTCGC AGACTGTCTA CGTCTGGTTA GATGCTTTAG TCAACTATCT CAGCTCTGTG 
GGATATCCAG ATGAAAAGTT TTCTGCCCAC TGGCCACCGG CGCAGCAAGT GATAGGGAAG 
GACATCCTCA AGTTCCATGG CATTTACTGG ACAGCTTTCC TCCTGGCTGC CGGCTTGGAA 
CCTCCTGGAC AGCTGTACGT TCACTCGCAT TGGACCGTCG ATGGGCAGAA GATGTCAAAG 
AGCAAGCACA ACGTGGTGGA TCCCCTCCAG GCAGCGCAGC AGTACACCAT GGAGGGCCTG 
CGATACTTCC TTCTGCGGGA GGGCGTGGCC CACAGCGATG GCAACTACAG CCACGTAAAG 
GCGCAGCGCA TCCTCAACTC GGAGCTGGCG GACACCCTGG GCAATCTGCT ATCGCGTGCC 
AGTGCCAAGT CGCTGAATCC CGGCCAAATC TATCCATCAC CCAGCGCGGA GCACTTGGCG 
GATCTCCTCC GGAGCCTGGA TGTGGCAAAA CGCCTGCAGG ATTCGCTTCT GCAGCTGTCA 
GAGCGATGCG AGTCGCATTA TGAGTGCAAT CACTTCCACT TGGTGGCCGA CACAACGATG 
GCGGCTCTGC ATGCGGCCAA CAACTTCTTC GAGAGCTCCA AGCCCTGGAC GCTAAAGGCG 
GGAGCACCGG ATGGCAACCA GGCCCGTTTG GAGACCATAA TCGCCATGAC AATGGACGCG 
TTGCGGCTCA GCGGCATTGT GTTGCAGCCC ATAATTCCCC AGCTGGCCAA CCGGCTGCTC 
GATAAGCTGA GCGTCCCCAC CGCGCAACGT GGCTGGAATT ACTTGGCCGA GAGTTTTGCC 
ACCAGCCCCA ACAGCTCCAA CAGTCCTGCG GGACTCGGCG AGAGCCGTCA ATTGGACGGG 
CAAACCAGTG CGCTGCTCTT CCAGCGGATT CTCGAAGAGA CGTCTGCGAA GGAAGTGAAG 
GAGCAGAAGC CGCAGCCAGC CAAGCGGAGC AAATCGAAGA AGAAGGAGCG CCGCGAAACA 
ATGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MetRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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