MARS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MARS2
Entrez Gene ID	92935
Full Name	methionyl-tRNA synthetase 2, mitochondrial
Synonyms	COXPD25,MetRS,mtMetRS
General protein information	Preferred Names methionyl-tRNA synthetase 2, mitochondrial Names methionine--tRNA ligase, mitochondrial methionine tRNA ligase 2, mitochondrial methionine--tRNA ligase 2 methionine-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.1
Summary	This gene produces a mitochondrial methionyl-tRNA synthetase protein that is encoded by the nuclear genome and imported to the mitochondrion. This protein likely functions as a monomer and is predicted to localize to the mitochondrial matrix. Mutations in this gene are associated with the autosomal recessive neurodegenerative disease spastic ataxia-3 (SPAX3). [provided by RefSeq, Apr 2014].
Disorder MIM:	609728
Disorder Html:	Spastic ataxia 3, autosomal recessive, 611390 (3); ?Combined oxidative phosphorylation deficiency 25, 616430 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_138395.4	NP_612404.1	methionine--tRNA ligase, mitochondrial precursor
NM_138395.3	NP_612404.1	methionine--tRNA ligase, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MARS2	XP_516010.3
	MARS2	XP_001086799.2
Bos taurus (cattle)	MARS2	NP_001092441.1
Rattus norvegicus (Norway rat)	LOC100911305	XP_003750747.1
Rattus norvegicus (Norway rat)	Mars2	XP_003750749.1
Danio rerio (zebrafish)	LOC566565	XP_694935.2
Caenorhabditis elegans (roundworm)	CELE_Y105E8A.20	NP_001129797.1
Kluyveromyces lactis	KLLA0F14971g	XP_455753.1
Eremothecium gossypii	AGOS_AAL133W	NP_982409.1
Oryza sativa (rice)	Os03g0209600	NP_001049336.1
Schizosaccharomyces pombe (fission yeast)	SPAC27E2.06c	NP_594407.1
Canis lupus familiaris (dog)	MARS2	XP_005640557.1
Gallus gallus (chicken)	MARS2	XP_426247.3
Neurospora crassa	NCU03759	XP_961106.1
Arabidopsis thaliana (thale cress)	OVA1	NP_191100.1
Xenopus tropicalis (tropical clawed frog)	LOC100488527	XP_002940449.2
Homo sapiens (human)	MARS2	NP_612404.1
Mus musculus (house mouse)	Mars2	NP_780648.1
Drosophila melanogaster (fruit fly)	Aats-met	NP_650348.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002383	XP_312573.4
Saccharomyces cerevisiae (baker's yeast)	MSM1	NP_011687.1
Magnaporthe oryzae (rice blast fungus)	MGG_16221	XP_003709691.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel, compound heterozygous, single-nucleotide variants in MARS2 associated with developmental delay, poor growth, and sensorineural hearing loss.
Webb BD, Wheeler PG, Hagen JJ, Cohen N, Linderman MD, Diaz GA, Naidich TP, Rodenburg RJ, Houten SM, Schadt EE
Human mutation36(6)587-92(2015 Jun)

Biological insights from 108 schizophrenia-associated genetic loci.

Nature511(7510)421-7(2014 Jul)

Mutations in the mitochondrial methionyl-tRNA synthetase cause a neurodegenerative phenotype in flies and a recessive ataxia (ARSAL) in humans.
Bayat V, Thiffault I, Jaiswal M, T?treault M, Donti T, Sasarman F, Bernard G, Demers-Lamarche J, Dicaire MJ, Mathieu J, Vanasse M, Bouchard JP, Rioux MF, Lourenco CM, Li Z, Haueter C, Shoubridge EA, Graham BH, Brais B, Bellen HJ
PLoS biology10(3)e1001288(2012)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Bonnefond L, Fender A, Rudinger-Thirion J, Gieg? R, Florentz C, Sissler M
Biochemistry44(12)4805-16(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Novel, compound heterozygous, single-nucleotide variants in MARS2 associated with developmental delay, poor growth, and sensorineural hearing loss
Title: Novel, compound heterozygous, single-nucleotide variants in MARS2 associated with developmental delay, poor growth, and sensorineural hearing loss.

MARS2 is mutated in Autosomal Recessive Spastic Ataxia with Leukoencephalopathy patients.
Title: Mutations in the mitochondrial methionyl-tRNA synthetase cause a neurodegenerative phenotype in flies and a recessive ataxia (ARSAL) in humans.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Sequence analysis of mitochondrial MetRS indicates that this protein contains consensus motifs characteristic of class I aminoacyl-tRNA synthetase but lacks Zn2+ binding motif and C-terminal dimerization region found in MetRSs from various organisms.
Title: Characterization of the human mitochondrial methionyl-tRNA synthetase.

The following MARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19513	NM_138395.4 Latest version!	Homo sapiens methionyl-tRNA synthetase 2, mitochondrial (MARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu19513	NM_138395.3	Homo sapiens methionyl-tRNA synthetase 2, mitochondrial (MARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MARS2 ( NM_138395.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_612404.1 Homo sapiens methionyl-tRNA synthetase 2, mitochondrial (MARS2), mRNA.

Price & Availability↑

CloneID	OHu19513
Clone ID Related Accession (Same CDS sequence)	NM_138395.4 , NM_138395.3
Accession Version	NM_138395.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	methionine--tRNA ligase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342319.1, BC009115.1, BC040934.2 and AC073058.4. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_138395.3. Sequence Note: This RefSeq contains an in-frame start site 20 codons upstream from the currently annotated site but is not being annotated as a start site since it is in a weak Kozak sequence context. ##Evidence-Data-START## Transcript is intronless :: AK098121.1, SRR1803613.126961.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000282276.8/ ENSP00000282276.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGAA CGTCCGTCCT CCGCCTGCTA GGACGCACGG GGGCTAGTAG GCTGTCTCTC 
CTGGAGGACT TCGGCCCACG CTACTACAGT TCGGGCTCCC TCAGTGCCGG CGATGATGCT 
TGTGATGTGC GCGCCTACTT CACTACACCC ATTTTCTACG TGAACGCGGC GCCGCACATC 
GGGCACCTGT ACTCGGCACT ACTGGCGGAC GCCCTATGCC GCCACCGTCG CCTCCGAGGT 
CCCAGCACGG CCGCCACGCG ATTCTCCACT GGTACCGACG AGCACGGGCT GAAGATTCAG 
CAGGCAGCAG CTACCGCGGG CCTGGCCCCG ACCGAGCTGT GCGACCGAGT CTCTGAGCAG 
TTCCAGCAGC TTTTCCAGGA GGCCGGTATC TCCTGCACAG ATTTCATCCG CACCACGGAG 
GCCCGGCACC GGGTGGCTGT GCAGCACTTC TGGGGGGTGC TTAAGTCCCG CGGTCTGCTC 
TACAAGGGCG TCTATGAAGG TTGGTATTGC GCTTCCGACG AGTGCTTCCT GCCTGAGGCC 
AAGGTCACCC AGCAGCCGGG CCCATCGGGG GATTCGTTTC CTGTATCTCT CGAGAGCGGG 
CATCCAGTCT CCTGGACCAA GGAAGAAAAC TACATTTTCA GGCTTTCCCA GTTCCGGAAG 
CCACTCCAGC GGTGGCTGCG GGGCAACCCT CAGGCGATCA CCCCCGAACC ATTTCATCAC 
GTAGTTCTTC AGTGGCTGGA CGAGGAGCTG CCCGACCTGT CCGTGTCTCG CAGAAGTAGC 
CACTTGCACT GGGGCATTCC GGTGCCCGGG GATGATTCGC AGACCATCTA TGTATGGCTG 
GATGCCCTGG TCAACTACCT CACTGTAATT GGCTACCCAA ATGCTGAGTT CAAATCTTGG 
TGGCCGGCCA CCTCTCATAT CATAGGTAAG GACATTCTCA AATTCCATGC CATCTATTGG 
CCTGCCTTCC TGTTAGGGGC CGGCATGAGC CCGCCACAGC GCATCTGTGT CCATTCCCAC 
TGGACAGTCT GTGGCCAAAA GATGTCCAAG AGCTTGGGCA ACGTGGTGGA TCCTAGGACT 
TGCCTTAACC GCTATACCGT GGATGGCTTC CGCTACTTTC TCCTTCGGCA GGGCGTCCCC 
AACTGGGACT GTGACTACTA TGATGAAAAG GTGGTTAAGT TGCTGAACTC CGAGCTGGCA 
GATGCCTTGG GAGGTCTCTT GAACCGATGC ACTGCCAAAA GAATAAATCC TTCTGAGACC 
TACCCAGCCT TCTGCACTAC CTGCTTCCCT AGTGAGCCAG GGTTGGTGGG GCCGTCAGTT 
CGTGCTCAGG CAGAGGATTA TGCTCTGGTG AGCGCAGTGG CCACTTTGCC AAAGCAGGTA 
GCAGACCACT ATGATAACTT TCGGATATAT AAGGCTCTGG AGGCCGTGTC CAGCTGTGTC 
CGGCAAACTA ATGGTTTTGT CCAAAGGCAT GCACCATGGA AGCTGAACTG GGAGAGCCCA 
GTGGATGCTC CCTGGCTGGG TACTGTGCTT CATGTGGCCT TGGAATGTTT GCGAGTCTTT 
GGGACTTTGC TGCAGCCTGT CACCCCAAGC CTAGCTGACA AGCTGCTGTC TAGGCTGGGG 
GTCTCTGCCT CAGAGAGGAG TCTTGGAGAG CTCTATTTCT TGCCTCGATT CTATGGACAT 
CCATGCCCTT TTGAAGGGAG GAGGCTGGGA CCTGAAACTG GGCTTTTGTT TCCAAGACTA 
GACCAGTCCA GGACTTGGCT GGTGAAAGCC CACCGGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612404.1
CDS	38..1819
Translation	MLRTSVLRLLGRTGASRLSLLEDFGPRYYSSGSLSAGDDACDVRAYFTTPIFYVNAAPHIGHLYSALLADALCRHRRLRGPSTAATRFSTGTDEHGLKIQQAAATAGLAPTELCDRVSEQFQQLFQEAGISCTDFIRTTEARHRVAVQHFWGVLKSRGLLYKGVYEGWYCASDECFLPEAKVTQQPGPSGDSFPVSLESGHPVSWTKEENYIFRLSQFRKPLQRWLRGNPQAITPEPFHHVVLQWLDEELPDLSVSRRSSHLHWGIPVPGDDSQTIYVWLDALVNYLTVIGYPNAEFKSWWPATSHIIGKDILKFHAIYWPAFLLGAGMSPPQRICVHSHWTVCGQKMSKSLGNVVDPRTCLNRYTVDGFRYFLLRQGVPNWDCDYYDEKVVKLLNSELADALGGLLNRCTAKRINPSETYPAFCTTCFPSEPGLVGPSVRAQAEDYALVSAVATLPKQVADHYDNFRIYKALEAVSSCVRQTNGFVQRHAPWKLNWESPVDAPWLGTVLHVALECLRVFGTLLQPVTPSLADKLLSRLGVSASERSLGELYFLPRFYGHPCPFEGRRLGPETGLLFPRLDQSRTWLVKAHRT

Target ORF information:

RefSeq Version	NM_138395.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl-tRNA synthetase 2, mitochondrial (MARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138395.4

ORF Insert Sequence:

ATGCTGCGAA CGTCCGTCCT CCGCCTGCTA GGACGCACGG GGGCTAGTAG GCTGTCTCTC 
CTGGAGGACT TCGGCCCACG CTACTACAGT TCGGGCTCCC TCAGTGCCGG CGATGATGCT 
TGTGATGTGC GCGCCTACTT CACTACACCC ATTTTCTACG TGAACGCGGC GCCGCACATC 
GGGCACCTGT ACTCGGCACT ACTGGCGGAC GCCCTATGCC GCCACCGTCG CCTCCGAGGT 
CCCAGCACGG CCGCCACGCG ATTCTCCACT GGTACCGACG AGCACGGGCT GAAGATTCAG 
CAGGCAGCAG CTACCGCGGG CCTGGCCCCG ACCGAGCTGT GCGACCGAGT CTCTGAGCAG 
TTCCAGCAGC TTTTCCAGGA GGCCGGTATC TCCTGCACAG ATTTCATCCG CACCACGGAG 
GCCCGGCACC GGGTGGCTGT GCAGCACTTC TGGGGGGTGC TTAAGTCCCG CGGTCTGCTC 
TACAAGGGCG TCTATGAAGG TTGGTATTGC GCTTCCGACG AGTGCTTCCT GCCTGAGGCC 
AAGGTCACCC AGCAGCCGGG CCCATCGGGG GATTCGTTTC CTGTATCTCT CGAGAGCGGG 
CATCCAGTCT CCTGGACCAA GGAAGAAAAC TACATTTTCA GGCTTTCCCA GTTCCGGAAG 
CCACTCCAGC GGTGGCTGCG GGGCAACCCT CAGGCGATCA CCCCCGAACC ATTTCATCAC 
GTAGTTCTTC AGTGGCTGGA CGAGGAGCTG CCCGACCTGT CCGTGTCTCG CAGAAGTAGC 
CACTTGCACT GGGGCATTCC GGTGCCCGGG GATGATTCGC AGACCATCTA TGTATGGCTG 
GATGCCCTGG TCAACTACCT CACTGTAATT GGCTACCCAA ATGCTGAGTT CAAATCTTGG 
TGGCCGGCCA CCTCTCATAT CATAGGTAAG GACATTCTCA AATTCCATGC CATCTATTGG 
CCTGCCTTCC TGTTAGGGGC CGGCATGAGC CCGCCACAGC GCATCTGTGT CCATTCCCAC 
TGGACAGTCT GTGGCCAAAA GATGTCCAAG AGCTTGGGCA ACGTGGTGGA TCCTAGGACT 
TGCCTTAACC GCTATACCGT GGATGGCTTC CGCTACTTTC TCCTTCGGCA GGGCGTCCCC 
AACTGGGACT GTGACTACTA TGATGAAAAG GTGGTTAAGT TGCTGAACTC CGAGCTGGCA 
GATGCCTTGG GAGGTCTCTT GAACCGATGC ACTGCCAAAA GAATAAATCC TTCTGAGACC 
TACCCAGCCT TCTGCACTAC CTGCTTCCCT AGTGAGCCAG GGTTGGTGGG GCCGTCAGTT 
CGTGCTCAGG CAGAGGATTA TGCTCTGGTG AGCGCAGTGG CCACTTTGCC AAAGCAGGTA 
GCAGACCACT ATGATAACTT TCGGATATAT AAGGCTCTGG AGGCCGTGTC CAGCTGTGTC 
CGGCAAACTA ATGGTTTTGT CCAAAGGCAT GCACCATGGA AGCTGAACTG GGAGAGCCCA 
GTGGATGCTC CCTGGCTGGG TACTGTGCTT CATGTGGCCT TGGAATGTTT GCGAGTCTTT 
GGGACTTTGC TGCAGCCTGT CACCCCAAGC CTAGCTGACA AGCTGCTGTC TAGGCTGGGG 
GTCTCTGCCT CAGAGAGGAG TCTTGGAGAG CTCTATTTCT TGCCTCGATT CTATGGACAT 
CCATGCCCTT TTGAAGGGAG GAGGCTGGGA CCTGAAACTG GGCTTTTGTT TCCAAGACTA 
GACCAGTCCA GGACTTGGCT GGTGAAAGCC CACCGGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARS2 ( NM_138395.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_612404.1 Homo sapiens methionyl-tRNA synthetase 2, mitochondrial (MARS2), mRNA.

Price & Availability↑

CloneID	OHu19513
Clone ID Related Accession (Same CDS sequence)	NM_138395.4 , NM_138395.3
Accession Version	NM_138395.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	methionine--tRNA ligase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342319.1, BC009115.1 and BC040934.2. This sequence is a reference standard in the RefSeqGene project. On Dec 22, 2010 this sequence version replaced NM_138395.2. Sequence Note: This RefSeq contains an in-frame start site 20 codons upstream from the currently annotated site but is not being annotated as a start site since it is in a weak Kozak sequence context. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGAA CGTCCGTCCT CCGCCTGCTA GGACGCACGG GGGCTAGTAG GCTGTCTCTC 
CTGGAGGACT TCGGCCCACG CTACTACAGT TCGGGCTCCC TCAGTGCCGG CGATGATGCT 
TGTGATGTGC GCGCCTACTT CACTACACCC ATTTTCTACG TGAACGCGGC GCCGCACATC 
GGGCACCTGT ACTCGGCACT ACTGGCGGAC GCCCTATGCC GCCACCGTCG CCTCCGAGGT 
CCCAGCACGG CCGCCACGCG ATTCTCCACT GGTACCGACG AGCACGGGCT GAAGATTCAG 
CAGGCAGCAG CTACCGCGGG CCTGGCCCCG ACCGAGCTGT GCGACCGAGT CTCTGAGCAG 
TTCCAGCAGC TTTTCCAGGA GGCCGGTATC TCCTGCACAG ATTTCATCCG CACCACGGAG 
GCCCGGCACC GGGTGGCTGT GCAGCACTTC TGGGGGGTGC TTAAGTCCCG CGGTCTGCTC 
TACAAGGGCG TCTATGAAGG TTGGTATTGC GCTTCCGACG AGTGCTTCCT GCCTGAGGCC 
AAGGTCACCC AGCAGCCGGG CCCATCGGGG GATTCGTTTC CTGTATCTCT CGAGAGCGGG 
CATCCAGTCT CCTGGACCAA GGAAGAAAAC TACATTTTCA GGCTTTCCCA GTTCCGGAAG 
CCACTCCAGC GGTGGCTGCG GGGCAACCCT CAGGCGATCA CCCCCGAACC ATTTCATCAC 
GTAGTTCTTC AGTGGCTGGA CGAGGAGCTG CCCGACCTGT CCGTGTCTCG CAGAAGTAGC 
CACTTGCACT GGGGCATTCC GGTGCCCGGG GATGATTCGC AGACCATCTA TGTATGGCTG 
GATGCCCTGG TCAACTACCT CACTGTAATT GGCTACCCAA ATGCTGAGTT CAAATCTTGG 
TGGCCGGCCA CCTCTCATAT CATAGGTAAG GACATTCTCA AATTCCATGC CATCTATTGG 
CCTGCCTTCC TGTTAGGGGC CGGCATGAGC CCGCCACAGC GCATCTGTGT CCATTCCCAC 
TGGACAGTCT GTGGCCAAAA GATGTCCAAG AGCTTGGGCA ACGTGGTGGA TCCTAGGACT 
TGCCTTAACC GCTATACCGT GGATGGCTTC CGCTACTTTC TCCTTCGGCA GGGCGTCCCC 
AACTGGGACT GTGACTACTA TGATGAAAAG GTGGTTAAGT TGCTGAACTC CGAGCTGGCA 
GATGCCTTGG GAGGTCTCTT GAACCGATGC ACTGCCAAAA GAATAAATCC TTCTGAGACC 
TACCCAGCCT TCTGCACTAC CTGCTTCCCT AGTGAGCCAG GGTTGGTGGG GCCGTCAGTT 
CGTGCTCAGG CAGAGGATTA TGCTCTGGTG AGCGCAGTGG CCACTTTGCC AAAGCAGGTA 
GCAGACCACT ATGATAACTT TCGGATATAT AAGGCTCTGG AGGCCGTGTC CAGCTGTGTC 
CGGCAAACTA ATGGTTTTGT CCAAAGGCAT GCACCATGGA AGCTGAACTG GGAGAGCCCA 
GTGGATGCTC CCTGGCTGGG TACTGTGCTT CATGTGGCCT TGGAATGTTT GCGAGTCTTT 
GGGACTTTGC TGCAGCCTGT CACCCCAAGC CTAGCTGACA AGCTGCTGTC TAGGCTGGGG 
GTCTCTGCCT CAGAGAGGAG TCTTGGAGAG CTCTATTTCT TGCCTCGATT CTATGGACAT 
CCATGCCCTT TTGAAGGGAG GAGGCTGGGA CCTGAAACTG GGCTTTTGTT TCCAAGACTA 
GACCAGTCCA GGACTTGGCT GGTGAAAGCC CACCGGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612404.1
CDS	103..1884
Translation	MLRTSVLRLLGRTGASRLSLLEDFGPRYYSSGSLSAGDDACDVRAYFTTPIFYVNAAPHIGHLYSALLADALCRHRRLRGPSTAATRFSTGTDEHGLKIQQAAATAGLAPTELCDRVSEQFQQLFQEAGISCTDFIRTTEARHRVAVQHFWGVLKSRGLLYKGVYEGWYCASDECFLPEAKVTQQPGPSGDSFPVSLESGHPVSWTKEENYIFRLSQFRKPLQRWLRGNPQAITPEPFHHVVLQWLDEELPDLSVSRRSSHLHWGIPVPGDDSQTIYVWLDALVNYLTVIGYPNAEFKSWWPATSHIIGKDILKFHAIYWPAFLLGAGMSPPQRICVHSHWTVCGQKMSKSLGNVVDPRTCLNRYTVDGFRYFLLRQGVPNWDCDYYDEKVVKLLNSELADALGGLLNRCTAKRINPSETYPAFCTTCFPSEPGLVGPSVRAQAEDYALVSAVATLPKQVADHYDNFRIYKALEAVSSCVRQTNGFVQRHAPWKLNWESPVDAPWLGTVLHVALECLRVFGTLLQPVTPSLADKLLSRLGVSASERSLGELYFLPRFYGHPCPFEGRRLGPETGLLFPRLDQSRTWLVKAHRT

Target ORF information:

RefSeq Version	NM_138395.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl-tRNA synthetase 2, mitochondrial (MARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138395.3

ORF Insert Sequence:

ATGCTGCGAA CGTCCGTCCT CCGCCTGCTA GGACGCACGG GGGCTAGTAG GCTGTCTCTC 
CTGGAGGACT TCGGCCCACG CTACTACAGT TCGGGCTCCC TCAGTGCCGG CGATGATGCT 
TGTGATGTGC GCGCCTACTT CACTACACCC ATTTTCTACG TGAACGCGGC GCCGCACATC 
GGGCACCTGT ACTCGGCACT ACTGGCGGAC GCCCTATGCC GCCACCGTCG CCTCCGAGGT 
CCCAGCACGG CCGCCACGCG ATTCTCCACT GGTACCGACG AGCACGGGCT GAAGATTCAG 
CAGGCAGCAG CTACCGCGGG CCTGGCCCCG ACCGAGCTGT GCGACCGAGT CTCTGAGCAG 
TTCCAGCAGC TTTTCCAGGA GGCCGGTATC TCCTGCACAG ATTTCATCCG CACCACGGAG 
GCCCGGCACC GGGTGGCTGT GCAGCACTTC TGGGGGGTGC TTAAGTCCCG CGGTCTGCTC 
TACAAGGGCG TCTATGAAGG TTGGTATTGC GCTTCCGACG AGTGCTTCCT GCCTGAGGCC 
AAGGTCACCC AGCAGCCGGG CCCATCGGGG GATTCGTTTC CTGTATCTCT CGAGAGCGGG 
CATCCAGTCT CCTGGACCAA GGAAGAAAAC TACATTTTCA GGCTTTCCCA GTTCCGGAAG 
CCACTCCAGC GGTGGCTGCG GGGCAACCCT CAGGCGATCA CCCCCGAACC ATTTCATCAC 
GTAGTTCTTC AGTGGCTGGA CGAGGAGCTG CCCGACCTGT CCGTGTCTCG CAGAAGTAGC 
CACTTGCACT GGGGCATTCC GGTGCCCGGG GATGATTCGC AGACCATCTA TGTATGGCTG 
GATGCCCTGG TCAACTACCT CACTGTAATT GGCTACCCAA ATGCTGAGTT CAAATCTTGG 
TGGCCGGCCA CCTCTCATAT CATAGGTAAG GACATTCTCA AATTCCATGC CATCTATTGG 
CCTGCCTTCC TGTTAGGGGC CGGCATGAGC CCGCCACAGC GCATCTGTGT CCATTCCCAC 
TGGACAGTCT GTGGCCAAAA GATGTCCAAG AGCTTGGGCA ACGTGGTGGA TCCTAGGACT 
TGCCTTAACC GCTATACCGT GGATGGCTTC CGCTACTTTC TCCTTCGGCA GGGCGTCCCC 
AACTGGGACT GTGACTACTA TGATGAAAAG GTGGTTAAGT TGCTGAACTC CGAGCTGGCA 
GATGCCTTGG GAGGTCTCTT GAACCGATGC ACTGCCAAAA GAATAAATCC TTCTGAGACC 
TACCCAGCCT TCTGCACTAC CTGCTTCCCT AGTGAGCCAG GGTTGGTGGG GCCGTCAGTT 
CGTGCTCAGG CAGAGGATTA TGCTCTGGTG AGCGCAGTGG CCACTTTGCC AAAGCAGGTA 
GCAGACCACT ATGATAACTT TCGGATATAT AAGGCTCTGG AGGCCGTGTC CAGCTGTGTC 
CGGCAAACTA ATGGTTTTGT CCAAAGGCAT GCACCATGGA AGCTGAACTG GGAGAGCCCA 
GTGGATGCTC CCTGGCTGGG TACTGTGCTT CATGTGGCCT TGGAATGTTT GCGAGTCTTT 
GGGACTTTGC TGCAGCCTGT CACCCCAAGC CTAGCTGACA AGCTGCTGTC TAGGCTGGGG 
GTCTCTGCCT CAGAGAGGAG TCTTGGAGAG CTCTATTTCT TGCCTCGATT CTATGGACAT 
CCATGCCCTT TTGAAGGGAG GAGGCTGGGA CCTGAAACTG GGCTTTTGTT TCCAAGACTA 
GACCAGTCCA GGACTTGGCT GGTGAAAGCC CACCGGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel, compound heterozygous, single-nucleotide variants in MARS2 associated with developmental delay, poor growth, and sensorineural hearing loss.
Human mutation36(6)587-92(2015 Jun)
Webb BD,Wheeler PG,Hagen JJ,Cohen N,Linderman MD,Diaz GA,Naidich TP,Rodenburg RJ,Houten SM,Schadt EE

Biological insights from 108 schizophrenia-associated genetic loci.
Nature511(7510)421-7(2014 Jul)

Mutations in the mitochondrial methionyl-tRNA synthetase cause a neurodegenerative phenotype in flies and a recessive ataxia (ARSAL) in humans.
PLoS biology10(3)e1001288(2012)
Bayat V,Thiffault I,Jaiswal M,T?treault M,Donti T,Sasarman F,Bernard G,Demers-Lamarche J,Dicaire MJ,Mathieu J,Vanasse M,Bouchard JP,Rioux MF,Lourenco CM,Li Z,Haueter C,Shoubridge EA,Graham BH,Brais B,Bellen HJ

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Biochemistry44(12)4805-16(2005 Mar)
Bonnefond L,Fender A,Rudinger-Thirion J,Gieg? R,Florentz C,Sissler M

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MARS2 cDNA ORF clone, Homo sapiens(human)

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