POP1 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene
Clones
gRNAs

Summary

Gene Symbol	POP1
Entrez Gene ID	2540511
Full Name	cullin 1 adaptor protein Pop1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe 972h-

mRNA and Protein(s)

mRNA	Protein	Name
NM_001355815.1	NP_001342944.1	cullin 1 adaptor protein Pop1
XM_001713094.2	XP_001713146.1	cullin 1 adaptor protein Pop1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj01805	XM_001713094.2 Latest version!	Schizosaccharomyces pombe 972h- cullin 1 adaptor protein Pop1 (pop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSc100124	NM_001355815.1 Latest version!	Schizosaccharomyces pombe cullin 1 adaptor protein Pop1 (pop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POP1 ( XM_001713094.2 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> XP_001713146.1 Schizosaccharomyces pombe 972h- cullin 1 adaptor protein Pop1 (pop1), mRNA.

Price & Availability↑

CloneID	OSj01805
Clone ID Related Accession (Same CDS sequence)	XM_001713094.2
Accession Version	XM_001713094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	cullin 1 adaptor protein Pop1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced XM_001713094.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAATGAGA AGAAAAAAGA TAGCCTATCT GTTTTAGATT CCAATGAATT TTTCGGTGAG 
ACAACTATGG TATCACCGAG TATTGATGTT TCCTCTTCTC CCCGGCCAAA TGTCGAGAGA 
TTTTCTCCAT GTTCCACTAA AAAAGATCTC CTGGAAGGAA ATAATATAAT GACCAGAATT 
CCCGAGGAGC TTAGTCGTGT TAGTCTACAA TTTGATAGCA AGGGAAGTCA GCAATCGATG 
ATATTTACTA ACAATCGATG TTTATCTGAT AAAGAAAATT TGGAAAACTT GCAAAATCTT 
TTATATCTAC ATTGTGATCT TAACCGGCCC CATTTGAGTT GTGAGCTGCC GTCAGAGCAT 
CGCGAAAAGT GCCTTAAACG CCGTAACTCT AGTCTCAGCT CTAATTTGCA TGCTAATAAG 
CGGTTTCTCT TCAATTCTCA AAGTGATGGG AATAAAAAGA ATGAAACTTT TCCTTCTACT 
AACTACTCGA ACGTTTTCTA CCCAAACAAC TGTGACAGTA AGGAAGTCGC TAGTGAAACA 
ACATTCTCAC TTGATGCACC AAATAATTCT GTTAATTACT CTTATTTTTC TCCTAATTTA 
CTCGGCAACG ATTCCAAAAC CCGTCAATCT TTTCCCCCTC ATTCATCTTC ATCTTCACAT 
AATTCTTTGC ATGAACCTGT CATATATGAT TTTTCTTCAG AAAATCCTAG CATTCATCCT 
TCTAATCATT TGAGCTCTCA AAAAAATGCT GTTTTGAAAT TAGCACAATT AATTTCTTCC 
TTCGAAAAAT TACCCGAAAG TGTTCGTCAA TACTTACTTT TTCATCTTCT TTCTCGATGT 
GGAAAACATG CTGTTCAAAA TATTCACAAA ATTCTGCTTC CTATCTTTCA AAAGAACTTT 
TTAACTGGAT TTCCGGCTGA GATTACAAAT TTAGTGCTTA CGCATTTAGA TGCACCTTCA 
TTGTGTGCAG TTTCGCAAGT TTCGCATCAC TGGTATAAGT TGGTATCGTC CAACGAAGAA 
CTTTGGAAAA GTCTCTTTTT AAAAGATGGT TTCTTTTGGG ATTCAATTGA TAGTAAAATC 
CGTACGATGT GTTTAGAACA ATCTTTGTCA GCCTGTGCGA TAATGAAGCG GGTCTATTTT 
AGACATTTTA ATCTTCGTGA AAGATGGCTC CATGCCCCGG AGAAAATTAA GCGATGTTCA 
TTCCCAATTC ATGGAGTTCG TCTTATTACC AAACTTCAGT TTGATGATGA CAAAATAATT 
GTTAGCACCT GCAGTCCTAG AATTAATATA TATGACACGA AAACAGGTGT CCTGATACGC 
AGTTTAGAAG AGCATGAAGG TGATGTTTGG ACTTTTGAAT ACGTTGGGGA TACTTTGGTT 
ACTGGCTCCA CAGACAGGAC AGTAAGAGTC TGGGACCTTA GAACCGGTGA ATGTAAACAG 
GTGTTTTATG GACACACGTC AACAATCCGT TGTATCAAAA TAGTTCAAGG CAATCAAAGT 
ACTACTGATA CTGATGATGT GGAAAAAGAA AACAGACCAG CTTCAAATGA TGCTAATTCA 
ATGCCTCCGT ATATAATTTC AAGTTCAAGG GATTGTACGA TCCGTTTATG GAGTCTTCCA 
TGCCTTGACG ATCCACCATT TGTTAATGTA AATGAAAATC CTGATCAGAA TAATGACTTT 
ACTTCAGCTA CGACCAATCC GTTTTACATC CGAACATTAC GTGGTCATAC GGACTCGGTA 
CGTGAAGTTG CTTGTCTTGG AGATTTGATT GTCAGTGCCA GTTATGATGG TACTCTTAGA 
GTATGGAAGG CATCTACTGG CGTTTGTTTA CATGTTCTTC GGGGACACGT CGGGAGAGTA 
TACAGTGTCA CAATTAATCC TTCTCGTCAA CAATGTATCA GCGCTGGTAC GGATGCTAAA 
ATTCGTATTT GGAACCTTGA GTCTGGAGAA CTTCTACAGA CCCTTCATGG TCATTCGAAT 
TTGGTAAGTC AAGTTACATT TAACCAAAAT ATCTTGGTAT CCGCTTCTGC TCCTCCTGAT 
ACATCGTTGA GAGTTTGGGA TCTAAACACC GGAAGTTGTC GAGATATTTT AAAATGTCCT 
TTGGGACACA TATTTTTTCA ACACGACGAG AGCAAAGTTG TCAGTGGAAG CCATAGTACG 
CTACAGTTAT GGGACATTCG TTCCGGAAAG TTAGTAAGAG ACCTATTGAC TGATTTGGAT 
ATAATCTGGC AAGTTGCTTA TAATGAAAAT GTATGTGTGG CTGCTGTTTT AAGAAATAAC 
CGTTTTTGGA TCGAGGTCCT AGAGTTTGGA TCTACTAAAT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713146.1
CDS	248..2575
Translation	MNEKKKDSLSVLDSNEFFGETTMVSPSIDVSSSPRPNVERFSPCSTKKDLLEGNNIMTRIPEELSRVSLQFDSKGSQQSMIFTNNRCLSDKENLENLQNLLYLHCDLNRPHLSCELPSEHREKCLKRRNSSLSSNLHANKRFLFNSQSDGNKKNETFPSTNYSNVFYPNNCDSKEVASETTFSLDAPNNSVNYSYFSPNLLGNDSKTRQSFPPHSSSSSHNSLHEPVIYDFSSENPSIHPSNHLSSQKNAVLKLAQLISSFEKLPESVRQYLLFHLLSRCGKHAVQNIHKILLPIFQKNFLTGFPAEITNLVLTHLDAPSLCAVSQVSHHWYKLVSSNEELWKSLFLKDGFFWDSIDSKIRTMCLEQSLSACAIMKRVYFRHFNLRERWLHAPEKIKRCSFPIHGVRLITKLQFDDDKIIVSTCSPRINIYDTKTGVLIRSLEEHEGDVWTFEYVGDTLVTGSTDRTVRVWDLRTGECKQVFYGHTSTIRCIKIVQGNQSTTDTDDVEKENRPASNDANSMPPYIISSSRDCTIRLWSLPCLDDPPFVNVNENPDQNNDFTSATTNPFYIRTLRGHTDSVREVACLGDLIVSASYDGTLRVWKASTGVCLHVLRGHVGRVYSVTINPSRQQCISAGTDAKIRIWNLESGELLQTLHGHSNLVSQVTFNQNILVSASAPPDTSLRVWDLNTGSCRDILKCPLGHIFFQHDESKVVSGSHSTLQLWDIRSGKLVRDLLTDLDIIWQVAYNENVCVAAVLRNNRFWIEVLEFGSTKSS

Target ORF information:

RefSeq Version	XM_001713094.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- cullin 1 adaptor protein Pop1 (pop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713094.2

ORF Insert Sequence:

ATGAATGAGA AGAAAAAAGA TAGCCTATCT GTTTTAGATT CCAATGAATT TTTCGGTGAG 
ACAACTATGG TATCACCGAG TATTGATGTT TCCTCTTCTC CCCGGCCAAA TGTCGAGAGA 
TTTTCTCCAT GTTCCACTAA AAAAGATCTC CTGGAAGGAA ATAATATAAT GACCAGAATT 
CCCGAGGAGC TTAGTCGTGT TAGTCTACAA TTTGATAGCA AGGGAAGTCA GCAATCGATG 
ATATTTACTA ACAATCGATG TTTATCTGAT AAAGAAAATT TGGAAAACTT GCAAAATCTT 
TTATATCTAC ATTGTGATCT TAACCGGCCC CATTTGAGTT GTGAGCTGCC GTCAGAGCAT 
CGCGAAAAGT GCCTTAAACG CCGTAACTCT AGTCTCAGCT CTAATTTGCA TGCTAATAAG 
CGGTTTCTCT TCAATTCTCA AAGTGATGGG AATAAAAAGA ATGAAACTTT TCCTTCTACT 
AACTACTCGA ACGTTTTCTA CCCAAACAAC TGTGACAGTA AGGAAGTCGC TAGTGAAACA 
ACATTCTCAC TTGATGCACC AAATAATTCT GTTAATTACT CTTATTTTTC TCCTAATTTA 
CTCGGCAACG ATTCCAAAAC CCGTCAATCT TTTCCCCCTC ATTCATCTTC ATCTTCACAT 
AATTCTTTGC ATGAACCTGT CATATATGAT TTTTCTTCAG AAAATCCTAG CATTCATCCT 
TCTAATCATT TGAGCTCTCA AAAAAATGCT GTTTTGAAAT TAGCACAATT AATTTCTTCC 
TTCGAAAAAT TACCCGAAAG TGTTCGTCAA TACTTACTTT TTCATCTTCT TTCTCGATGT 
GGAAAACATG CTGTTCAAAA TATTCACAAA ATTCTGCTTC CTATCTTTCA AAAGAACTTT 
TTAACTGGAT TTCCGGCTGA GATTACAAAT TTAGTGCTTA CGCATTTAGA TGCACCTTCA 
TTGTGTGCAG TTTCGCAAGT TTCGCATCAC TGGTATAAGT TGGTATCGTC CAACGAAGAA 
CTTTGGAAAA GTCTCTTTTT AAAAGATGGT TTCTTTTGGG ATTCAATTGA TAGTAAAATC 
CGTACGATGT GTTTAGAACA ATCTTTGTCA GCCTGTGCGA TAATGAAGCG GGTCTATTTT 
AGACATTTTA ATCTTCGTGA AAGATGGCTC CATGCCCCGG AGAAAATTAA GCGATGTTCA 
TTCCCAATTC ATGGAGTTCG TCTTATTACC AAACTTCAGT TTGATGATGA CAAAATAATT 
GTTAGCACCT GCAGTCCTAG AATTAATATA TATGACACGA AAACAGGTGT CCTGATACGC 
AGTTTAGAAG AGCATGAAGG TGATGTTTGG ACTTTTGAAT ACGTTGGGGA TACTTTGGTT 
ACTGGCTCCA CAGACAGGAC AGTAAGAGTC TGGGACCTTA GAACCGGTGA ATGTAAACAG 
GTGTTTTATG GACACACGTC AACAATCCGT TGTATCAAAA TAGTTCAAGG CAATCAAAGT 
ACTACTGATA CTGATGATGT GGAAAAAGAA AACAGACCAG CTTCAAATGA TGCTAATTCA 
ATGCCTCCGT ATATAATTTC AAGTTCAAGG GATTGTACGA TCCGTTTATG GAGTCTTCCA 
TGCCTTGACG ATCCACCATT TGTTAATGTA AATGAAAATC CTGATCAGAA TAATGACTTT 
ACTTCAGCTA CGACCAATCC GTTTTACATC CGAACATTAC GTGGTCATAC GGACTCGGTA 
CGTGAAGTTG CTTGTCTTGG AGATTTGATT GTCAGTGCCA GTTATGATGG TACTCTTAGA 
GTATGGAAGG CATCTACTGG CGTTTGTTTA CATGTTCTTC GGGGACACGT CGGGAGAGTA 
TACAGTGTCA CAATTAATCC TTCTCGTCAA CAATGTATCA GCGCTGGTAC GGATGCTAAA 
ATTCGTATTT GGAACCTTGA GTCTGGAGAA CTTCTACAGA CCCTTCATGG TCATTCGAAT 
TTGGTAAGTC AAGTTACATT TAACCAAAAT ATCTTGGTAT CCGCTTCTGC TCCTCCTGAT 
ACATCGTTGA GAGTTTGGGA TCTAAACACC GGAAGTTGTC GAGATATTTT AAAATGTCCT 
TTGGGACACA TATTTTTTCA ACACGACGAG AGCAAAGTTG TCAGTGGAAG CCATAGTACG 
CTACAGTTAT GGGACATTCG TTCCGGAAAG TTAGTAAGAG ACCTATTGAC TGATTTGGAT 
ATAATCTGGC AAGTTGCTTA TAATGAAAAT GTATGTGTGG CTGCTGTTTT AAGAAATAAC 
CGTTTTTGGA TCGAGGTCCT AGAGTTTGGA TCTACTAAAT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POP1 ( NM_001355815.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001342944.1 Schizosaccharomyces pombe cullin 1 adaptor protein Pop1 (pop1), mRNA.

Price & Availability↑

CloneID	OSc100124
Clone ID Related Accession (Same CDS sequence)	NM_001355815.1
Accession Version	NM_001355815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	cullin 1 adaptor protein Pop1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

ATGAATGAGA AGAAAAAAGA TAGCCTATCT GTTTTAGATT CCAATGAATT TTTCGGTGAG 
ACAACTATGG TATCACCGAG TATTGATGTT TCCTCTTCTC CCCGGCCAAA TGTCGAGAGA 
TTTTCTCCAT GTTCCACTAA AAAAGATCTC CTGGAAGGAA ATAATATAAT GACCAGAATT 
CCCGAGGAGC TTAGTCGTGT TAGTCTACAA TTTGATAGCA AGGGAAGTCA GCAATCGATG 
ATATTTACTA ACAATCGATG TTTATCTGAT AAAGAAAATT TGGAAAACTT GCAAAATCTT 
TTATATCTAC ATTGTGATCT TAACCGGCCC CATTTGAGTT GTGAGCTGCC GTCAGAGCAT 
CGCGAAAAGT GCCTTAAACG CCGTAACTCT AGTCTCAGCT CTAATTTGCA TGCTAATAAG 
CGGTTTCTCT TCAATTCTCA AAGTGATGGG AATAAAAAGA ATGAAACTTT TCCTTCTACT 
AACTACTCGA ACGTTTTCTA CCCAAACAAC TGTGACAGTA AGGAAGTCGC TAGTGAAACA 
ACATTCTCAC TTGATGCACC AAATAATTCT GTTAATTACT CTTATTTTTC TCCTAATTTA 
CTCGGCAACG ATTCCAAAAC CCGTCAATCT TTTCCCCCTC ATTCATCTTC ATCTTCACAT 
AATTCTTTGC ATGAACCTGT CATATATGAT TTTTCTTCAG AAAATCCTAG CATTCATCCT 
TCTAATCATT TGAGCTCTCA AAAAAATGCT GTTTTGAAAT TAGCACAATT AATTTCTTCC 
TTCGAAAAAT TACCCGAAAG TGTTCGTCAA TACTTACTTT TTCATCTTCT TTCTCGATGT 
GGAAAACATG CTGTTCAAAA TATTCACAAA ATTCTGCTTC CTATCTTTCA AAAGAACTTT 
TTAACTGGAT TTCCGGCTGA GATTACAAAT TTAGTGCTTA CGCATTTAGA TGCACCTTCA 
TTGTGTGCAG TTTCGCAAGT TTCGCATCAC TGGTATAAGT TGGTATCGTC CAACGAAGAA 
CTTTGGAAAA GTCTCTTTTT AAAAGATGGT TTCTTTTGGG ATTCAATTGA TAGTAAAATC 
CGTACGATGT GTTTAGAACA ATCTTTGTCA GCCTGTGCGA TAATGAAGCG GGTCTATTTT 
AGACATTTTA ATCTTCGTGA AAGATGGCTC CATGCCCCGG AGAAAATTAA GCGATGTTCA 
TTCCCAATTC ATGGAGTTCG TCTTATTACC AAACTTCAGT TTGATGATGA CAAAATAATT 
GTTAGCACCT GCAGTCCTAG AATTAATATA TATGACACGA AAACAGGTGT CCTGATACGC 
AGTTTAGAAG AGCATGAAGG TGATGTTTGG ACTTTTGAAT ACGTTGGGGA TACTTTGGTT 
ACTGGCTCCA CAGACAGGAC AGTAAGAGTC TGGGACCTTA GAACCGGTGA ATGTAAACAG 
GTGTTTTATG GACACACGTC AACAATCCGT TGTATCAAAA TAGTTCAAGG CAATCAAAGT 
ACTACTGATA CTGATGATGT GGAAAAAGAA AACAGACCAG CTTCAAATGA TGCTAATTCA 
ATGCCTCCGT ATATAATTTC AAGTTCAAGG GATTGTACGA TCCGTTTATG GAGTCTTCCA 
TGCCTTGACG ATCCACCATT TGTTAATGTA AATGAAAATC CTGATCAGAA TAATGACTTT 
ACTTCAGCTA CGACCAATCC GTTTTACATC CGAACATTAC GTGGTCATAC GGACTCGGTA 
CGTGAAGTTG CTTGTCTTGG AGATTTGATT GTCAGTGCCA GTTATGATGG TACTCTTAGA 
GTATGGAAGG CATCTACTGG CGTTTGTTTA CATGTTCTTC GGGGACACGT CGGGAGAGTA 
TACAGTGTCA CAATTAATCC TTCTCGTCAA CAATGTATCA GCGCTGGTAC GGATGCTAAA 
ATTCGTATTT GGAACCTTGA GTCTGGAGAA CTTCTACAGA CCCTTCATGG TCATTCGAAT 
TTGGTAAGTC AAGTTACATT TAACCAAAAT ATCTTGGTAT CCGCTTCTGC TCCTCCTGAT 
ACATCGTTGA GAGTTTGGGA TCTAAACACC GGAAGTTGTC GAGATATTTT AAAATGTCCT 
TTGGGACACA TATTTTTTCA ACACGACGAG AGCAAAGTTG TCAGTGGAAG CCATAGTACG 
CTACAGTTAT GGGACATTCG TTCCGGAAAG TTAGTAAGAG ACCTATTGAC TGATTTGGAT 
ATAATCTGGC AAGTTGCTTA TAATGAAAAT GTATGTGTGG CTGCTGTTTT AAGAAATAAC 
CGTTTTTGGA TCGAGGTCCT AGAGTTTGGA TCTACTAAAT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342944.1
CDS	248..2575
Translation	MNEKKKDSLSVLDSNEFFGETTMVSPSIDVSSSPRPNVERFSPCSTKKDLLEGNNIMTRIPEELSRVSLQFDSKGSQQSMIFTNNRCLSDKENLENLQNLLYLHCDLNRPHLSCELPSEHREKCLKRRNSSLSSNLHANKRFLFNSQSDGNKKNETFPSTNYSNVFYPNNCDSKEVASETTFSLDAPNNSVNYSYFSPNLLGNDSKTRQSFPPHSSSSSHNSLHEPVIYDFSSENPSIHPSNHLSSQKNAVLKLAQLISSFEKLPESVRQYLLFHLLSRCGKHAVQNIHKILLPIFQKNFLTGFPAEITNLVLTHLDAPSLCAVSQVSHHWYKLVSSNEELWKSLFLKDGFFWDSIDSKIRTMCLEQSLSACAIMKRVYFRHFNLRERWLHAPEKIKRCSFPIHGVRLITKLQFDDDKIIVSTCSPRINIYDTKTGVLIRSLEEHEGDVWTFEYVGDTLVTGSTDRTVRVWDLRTGECKQVFYGHTSTIRCIKIVQGNQSTTDTDDVEKENRPASNDANSMPPYIISSSRDCTIRLWSLPCLDDPPFVNVNENPDQNNDFTSATTNPFYIRTLRGHTDSVREVACLGDLIVSASYDGTLRVWKASTGVCLHVLRGHVGRVYSVTINPSRQQCISAGTDAKIRIWNLESGELLQTLHGHSNLVSQVTFNQNILVSASAPPDTSLRVWDLNTGSCRDILKCPLGHIFFQHDESKVVSGSHSTLQLWDIRSGKLVRDLLTDLDIIWQVAYNENVCVAAVLRNNRFWIEVLEFGSTKSS

Target ORF information:

RefSeq Version	NM_001355815.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe cullin 1 adaptor protein Pop1 (pop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355815.1

ORF Insert Sequence:

ATGAATGAGA AGAAAAAAGA TAGCCTATCT GTTTTAGATT CCAATGAATT TTTCGGTGAG 
ACAACTATGG TATCACCGAG TATTGATGTT TCCTCTTCTC CCCGGCCAAA TGTCGAGAGA 
TTTTCTCCAT GTTCCACTAA AAAAGATCTC CTGGAAGGAA ATAATATAAT GACCAGAATT 
CCCGAGGAGC TTAGTCGTGT TAGTCTACAA TTTGATAGCA AGGGAAGTCA GCAATCGATG 
ATATTTACTA ACAATCGATG TTTATCTGAT AAAGAAAATT TGGAAAACTT GCAAAATCTT 
TTATATCTAC ATTGTGATCT TAACCGGCCC CATTTGAGTT GTGAGCTGCC GTCAGAGCAT 
CGCGAAAAGT GCCTTAAACG CCGTAACTCT AGTCTCAGCT CTAATTTGCA TGCTAATAAG 
CGGTTTCTCT TCAATTCTCA AAGTGATGGG AATAAAAAGA ATGAAACTTT TCCTTCTACT 
AACTACTCGA ACGTTTTCTA CCCAAACAAC TGTGACAGTA AGGAAGTCGC TAGTGAAACA 
ACATTCTCAC TTGATGCACC AAATAATTCT GTTAATTACT CTTATTTTTC TCCTAATTTA 
CTCGGCAACG ATTCCAAAAC CCGTCAATCT TTTCCCCCTC ATTCATCTTC ATCTTCACAT 
AATTCTTTGC ATGAACCTGT CATATATGAT TTTTCTTCAG AAAATCCTAG CATTCATCCT 
TCTAATCATT TGAGCTCTCA AAAAAATGCT GTTTTGAAAT TAGCACAATT AATTTCTTCC 
TTCGAAAAAT TACCCGAAAG TGTTCGTCAA TACTTACTTT TTCATCTTCT TTCTCGATGT 
GGAAAACATG CTGTTCAAAA TATTCACAAA ATTCTGCTTC CTATCTTTCA AAAGAACTTT 
TTAACTGGAT TTCCGGCTGA GATTACAAAT TTAGTGCTTA CGCATTTAGA TGCACCTTCA 
TTGTGTGCAG TTTCGCAAGT TTCGCATCAC TGGTATAAGT TGGTATCGTC CAACGAAGAA 
CTTTGGAAAA GTCTCTTTTT AAAAGATGGT TTCTTTTGGG ATTCAATTGA TAGTAAAATC 
CGTACGATGT GTTTAGAACA ATCTTTGTCA GCCTGTGCGA TAATGAAGCG GGTCTATTTT 
AGACATTTTA ATCTTCGTGA AAGATGGCTC CATGCCCCGG AGAAAATTAA GCGATGTTCA 
TTCCCAATTC ATGGAGTTCG TCTTATTACC AAACTTCAGT TTGATGATGA CAAAATAATT 
GTTAGCACCT GCAGTCCTAG AATTAATATA TATGACACGA AAACAGGTGT CCTGATACGC 
AGTTTAGAAG AGCATGAAGG TGATGTTTGG ACTTTTGAAT ACGTTGGGGA TACTTTGGTT 
ACTGGCTCCA CAGACAGGAC AGTAAGAGTC TGGGACCTTA GAACCGGTGA ATGTAAACAG 
GTGTTTTATG GACACACGTC AACAATCCGT TGTATCAAAA TAGTTCAAGG CAATCAAAGT 
ACTACTGATA CTGATGATGT GGAAAAAGAA AACAGACCAG CTTCAAATGA TGCTAATTCA 
ATGCCTCCGT ATATAATTTC AAGTTCAAGG GATTGTACGA TCCGTTTATG GAGTCTTCCA 
TGCCTTGACG ATCCACCATT TGTTAATGTA AATGAAAATC CTGATCAGAA TAATGACTTT 
ACTTCAGCTA CGACCAATCC GTTTTACATC CGAACATTAC GTGGTCATAC GGACTCGGTA 
CGTGAAGTTG CTTGTCTTGG AGATTTGATT GTCAGTGCCA GTTATGATGG TACTCTTAGA 
GTATGGAAGG CATCTACTGG CGTTTGTTTA CATGTTCTTC GGGGACACGT CGGGAGAGTA 
TACAGTGTCA CAATTAATCC TTCTCGTCAA CAATGTATCA GCGCTGGTAC GGATGCTAAA 
ATTCGTATTT GGAACCTTGA GTCTGGAGAA CTTCTACAGA CCCTTCATGG TCATTCGAAT 
TTGGTAAGTC AAGTTACATT TAACCAAAAT ATCTTGGTAT CCGCTTCTGC TCCTCCTGAT 
ACATCGTTGA GAGTTTGGGA TCTAAACACC GGAAGTTGTC GAGATATTTT AAAATGTCCT 
TTGGGACACA TATTTTTTCA ACACGACGAG AGCAAAGTTG TCAGTGGAAG CCATAGTACG 
CTACAGTTAT GGGACATTCG TTCCGGAAAG TTAGTAAGAG ACCTATTGAC TGATTTGGAT 
ATAATCTGGC AAGTTGCTTA TAATGAAAAT GTATGTGTGG CTGCTGTTTT AAGAAATAAC 
CGTTTTTGGA TCGAGGTCCT AGAGTTTGGA TCTACTAAAT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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POP1 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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