gef3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	gef3
Entrez Gene ID	2540334
Full Name	RhoGEF Gef3
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021749.2	NP_595845.1	RhoGEF Gef3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Rho guanine nucleotide exchange factors Gef3 interacts with the septin complex and activates Rho4 GTPase for septation during cytokinesis.
Title: The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.

Gef3p contains a putative DH homology domain and a BAR/IMD-like domain. The protein localized to the division site late in mitosis, where it formed a ring that did not constrict with actomyosin ring.
Title: The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.

The following gef3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gef3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100512	NM_001021749.2 Latest version!	Schizosaccharomyces pombe RhoGEF Gef3 (gef3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gef3 ( NM_001021749.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595845.1 Schizosaccharomyces pombe RhoGEF Gef3 (gef3), mRNA.

Price & Availability↑

CloneID	OSc100512
Clone ID Related Accession (Same CDS sequence)	NM_001021749.2
Accession Version	NM_001021749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	RhoGEF Gef3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021749.1.

ATGAGTAACC CAAGGATGTT CCAAAGCCGT GGAAAAAGAG TTGTTTCAGA TAGCTTGCCT 
ATAGCCAATT CGACACACAC TTTAGAAGAT ACTTTGAAAT TACGGCCTGA CTGGACCATA 
GCGTTAAGTC AACATGAAAC TGAAACAAGG TCGACAAGAA CTAGTTGTAG CACTACCACA 
GAATCGACGA TTGCTATGAG GAGTAAACAA AAAGCCATTA TTTCTGTTCT GGAGGAATTT 
CGTGATTCAG AAGCCGCTTA CGTTCATGAT CTGCATGTAG CTCAACGGTA CTATGCTGAC 
CGATTATCGG ACCGAGTAAA AAAAAGCGAA TGGAAAGATG TGTTTGAGAT TTTTCTGGTA 
TTGTGCAAGC AAGCATCACT TTTCGAAATC GAAATGCACA AATCCTTGAA CGACGAAATC 
AATTACATTT TGGATGATCA AGATGCCTGT TTGAAGCCTA AGCCTTCCGT TGCACAACTA 
TTTCTTTCAT GGTTGCCCAA GCTATCTGCT GTCTATGGTC GATACTGCGT GATTCAAGAA 
AACATTGGCA AAAAGGTGGA AAAATGGATG AAGAACTCAT CAATCTCAGA ATATTTGCAG 
GAATGTGATA GCATGGCAAA AATTGAAAGT AACTCTTGGA ATTTAGATAG TTTTTTAGTA 
AAGCCTGTGC AGCGTTTTCT GAAGTATCCG CTTTTACTAA ATCAGCTTTA CCGATCTGCT 
TCACTGGGTA TCATTTCCGA CTATGTGTTG CTCGGGGAAG CTTGTCACAA ATCTGAGATC 
GCTAGCCAAA GAATGAATGA GTTAAAAAGG AGACGAGATA TTATAATTAC AGCCCTGGAT 
AGCGTCTCGA ATTCCCAAGA GGTCTTACTT CTCTCCACAG ATTCAATTGA CAAGAAAATT 
GCAAAGCTTC AAAATTCTAC AAACATTTTC TACGTACCTG AACATGAGCC CATTCTAGCG 
TTTGTCCATC AACTGTCTTC ATCCTATACC AATCTTTTAA ATTTACGAAG TGCTATCTGT 
GATTGGTTAA AATTCTCCAG ATATCATTAC CTAAAATTCT TTACCTTCGT CGAAGCCTAT 
TCTGTGTTTT GTAAAGACAC TAAATCTGCA GACAAATGGG CTCTCATTAG TGTAGCATTA 
GACAACATTG CCAAGGGTGC AGTGCTTCGT TTGACAGAGC AATGTCAAAC ATCCGTTTTG 
AGACCTATTT CCAATGGTAT TCTTTTCTTC CGAAACCCCC TTTGTGTTAC CGATGTATGG 
ATCAAAAAAG CTACAGCATT TTCCAAGCGA CGCCAATCTC AGGTTTTTGA AGAGGACCTT 
GAAAGCTTCC CTTTACTTAG TAATTGCCTT TTAGAAGAAC TTCCTCTTTT TTTGGAGATG 
GCGCGTAATG TTACCGACGA ATGTATATTA GCATTTGCTC AAATTCAAGC TACCTTTTAC 
GATACCATTC AGAAAGTCCT TGAACCTGTC GTTGCAAAAT TCAACTTAAC TGACCACCAG 
GATATTCCAT CGATTGAATC CATAATGGAT TTCACCAGTT TGCGGTCTAG TATGGAGTCC 
TCCCCAAAAT CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595845.1
CDS	240..1817
Translation	MSNPRMFQSRGKRVVSDSLPIANSTHTLEDTLKLRPDWTIALSQHETETRSTRTSCSTTTESTIAMRSKQKAIISVLEEFRDSEAAYVHDLHVAQRYYADRLSDRVKKSEWKDVFEIFLVLCKQASLFEIEMHKSLNDEINYILDDQDACLKPKPSVAQLFLSWLPKLSAVYGRYCVIQENIGKKVEKWMKNSSISEYLQECDSMAKIESNSWNLDSFLVKPVQRFLKYPLLLNQLYRSASLGIISDYVLLGEACHKSEIASQRMNELKRRRDIIITALDSVSNSQEVLLLSTDSIDKKIAKLQNSTNIFYVPEHEPILAFVHQLSSSYTNLLNLRSAICDWLKFSRYHYLKFFTFVEAYSVFCKDTKSADKWALISVALDNIAKGAVLRLTEQCQTSVLRPISNGILFFRNPLCVTDVWIKKATAFSKRRQSQVFEEDLESFPLLSNCLLEELPLFLEMARNVTDECILAFAQIQATFYDTIQKVLEPVVAKFNLTDHQDIPSIESIMDFTSLRSSMESSPKSK

Target ORF information:

RefSeq Version	NM_001021749.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe RhoGEF Gef3 (gef3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021749.2

ORF Insert Sequence:

ATGAGTAACC CAAGGATGTT CCAAAGCCGT GGAAAAAGAG TTGTTTCAGA TAGCTTGCCT 
ATAGCCAATT CGACACACAC TTTAGAAGAT ACTTTGAAAT TACGGCCTGA CTGGACCATA 
GCGTTAAGTC AACATGAAAC TGAAACAAGG TCGACAAGAA CTAGTTGTAG CACTACCACA 
GAATCGACGA TTGCTATGAG GAGTAAACAA AAAGCCATTA TTTCTGTTCT GGAGGAATTT 
CGTGATTCAG AAGCCGCTTA CGTTCATGAT CTGCATGTAG CTCAACGGTA CTATGCTGAC 
CGATTATCGG ACCGAGTAAA AAAAAGCGAA TGGAAAGATG TGTTTGAGAT TTTTCTGGTA 
TTGTGCAAGC AAGCATCACT TTTCGAAATC GAAATGCACA AATCCTTGAA CGACGAAATC 
AATTACATTT TGGATGATCA AGATGCCTGT TTGAAGCCTA AGCCTTCCGT TGCACAACTA 
TTTCTTTCAT GGTTGCCCAA GCTATCTGCT GTCTATGGTC GATACTGCGT GATTCAAGAA 
AACATTGGCA AAAAGGTGGA AAAATGGATG AAGAACTCAT CAATCTCAGA ATATTTGCAG 
GAATGTGATA GCATGGCAAA AATTGAAAGT AACTCTTGGA ATTTAGATAG TTTTTTAGTA 
AAGCCTGTGC AGCGTTTTCT GAAGTATCCG CTTTTACTAA ATCAGCTTTA CCGATCTGCT 
TCACTGGGTA TCATTTCCGA CTATGTGTTG CTCGGGGAAG CTTGTCACAA ATCTGAGATC 
GCTAGCCAAA GAATGAATGA GTTAAAAAGG AGACGAGATA TTATAATTAC AGCCCTGGAT 
AGCGTCTCGA ATTCCCAAGA GGTCTTACTT CTCTCCACAG ATTCAATTGA CAAGAAAATT 
GCAAAGCTTC AAAATTCTAC AAACATTTTC TACGTACCTG AACATGAGCC CATTCTAGCG 
TTTGTCCATC AACTGTCTTC ATCCTATACC AATCTTTTAA ATTTACGAAG TGCTATCTGT 
GATTGGTTAA AATTCTCCAG ATATCATTAC CTAAAATTCT TTACCTTCGT CGAAGCCTAT 
TCTGTGTTTT GTAAAGACAC TAAATCTGCA GACAAATGGG CTCTCATTAG TGTAGCATTA 
GACAACATTG CCAAGGGTGC AGTGCTTCGT TTGACAGAGC AATGTCAAAC ATCCGTTTTG 
AGACCTATTT CCAATGGTAT TCTTTTCTTC CGAAACCCCC TTTGTGTTAC CGATGTATGG 
ATCAAAAAAG CTACAGCATT TTCCAAGCGA CGCCAATCTC AGGTTTTTGA AGAGGACCTT 
GAAAGCTTCC CTTTACTTAG TAATTGCCTT TTAGAAGAAC TTCCTCTTTT TTTGGAGATG 
GCGCGTAATG TTACCGACGA ATGTATATTA GCATTTGCTC AAATTCAAGC TACCTTTTAC 
GATACCATTC AGAAAGTCCT TGAACCTGTC GTTGCAAAAT TCAACTTAAC TGACCACCAG 
GATATTCCAT CGATTGAATC CATAATGGAT TTCACCAGTT TGCGGTCTAG TATGGAGTCC 
TCCCCAAAAT CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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gef3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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