atf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	atf1
Entrez Gene ID	2540329
Full Name	transcription factor, Atf-CREB family Atf1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021546.2	NP_595652.1	transcription factor, Atf-CREB family Atf1
NM_001356227.1	NP_001342974.1	Atf-CREB family transcription factor Atf1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that S. pombe 972 expressing a nonphosphorylatable Atf1 variant is more sensitive to oxidative stress because of impaired transcription of subset of stress genes whose expression is controlled by Pap1; in S. pombe 972 expressing phospho-mimicking Atf1 mutant, presence of Sty1 is completely dispensable. (Atf1 = transcription factor Atf1; Sty1 = MAP kinase Sty1; Pap1 = transcription factor Pap1)
Title: Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation.

A group of hotspots in fission yeast are associated with sequences similar to the cyclic AMP response element and activated by the ATF/CREB family transcription factor dimer Atf1-Pcr1.
Title: Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots.

Atf1 binds to the Cdc13 promoter, leading to activation of Cdc13 expression.
Title: The basic leucine zipper domain transcription factor Atf1 directly controls Cdc13 expression and regulates mitotic entry independently of Wee1 and Cdc25 in Schizosaccharomyces pombe.

The data indicates that the Atf1 transcription factor binding to promoters acts as an effective barrier to fix the +1 nucleosome and phase downstream nucleosome arrays to prevent cryptic transcription.
Title: Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription.

The data indicate that Atf1 and Pcr1 induce the transcription of agl1, which allows efficient utilization of extracellular maltose.
Title: The transcription factors Atf1 and Pcr1 are essential for transcriptional induction of the extracellular maltase Agl1 in fission yeast.

The following atf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj01625	NM_001021546.2 Latest version!	Schizosaccharomyces pombe 972h- transcription factor, Atf-CREB family Atf1 (atf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OSc100210	NM_001356227.1 Latest version!	Schizosaccharomyces pombe Atf-CREB family transcription factor Atf1 (atf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atf1 ( NM_001021546.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595652.1 Schizosaccharomyces pombe 972h- transcription factor, Atf-CREB family Atf1 (atf1), mRNA.

Price & Availability↑

CloneID	OSj01625
Clone ID Related Accession (Same CDS sequence)	NM_001021546.2
Accession Version	NM_001021546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	transcription factor, Atf-CREB family Atf1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021546.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGTCCCCGT CTCCCGTCAA TACTTCCACA GAACCAGCAT CTGTTGCCGC TGTTTCAAAT 
GGTAATGCTA CTGCTTCCAG TACTCAGGTT CCGGAAAACA ATCAATCTGA TTCATTTGCA 
CCACCAAGCA ACAATTCTCA GCAGAATCAA CAGTCTTCAA CTATCGCTCC TAATGGAGGG 
GCCGGATCTG TCGCTAATGC GAATCCAGCT GACCAATCCG ATGGTGTAAC TCCGTCTTTC 
GTAGGCTCCT TGAAGCTGGA TTACGAACCC AACCCCTTTG AGCATTCATT TGGCTCTACC 
GCTTCGGTTG GACAAGGAAA TCCTTCTCTT AATCGTAATC CTAGCCTTAG TAACATTCCT 
TCAGGTGTGC CTCCTGCGTT TGCGCGAACT CTCTTACCTC CTGTTTCTTC CATAGCATCA 
CCTGATATCT TAAGTGGAGC TCCAGGTATT GCCTCGCCGT TGGGCTATCC TGCTTGGTCT 
GCTTTTACTC GTGGTACAAT GCATAATCCC CTTTCTCCTG CTATTTATGA TGCTACTCTT 
CGTCCTGACT ACCTAAACAA TCCTTCAGAT GCATCTGCAG CAGCTAGATT TTCTAGTGGA 
ACTGGATTTA CTCCTGGTGT TAATGAACCC TTCCGATCTC TTTTAACCCC TACTGGAGCT 
GGATTTCCCG CTCCTTCACC TGGTACTGCC AATTTATTAG GGTTTCATAC GTTTGATTCT 
CAATTTCCTG ATCAATATCG TTTTACCCCA AGGGATGGTA AACCGCCTGT TGTAAATGGG 
ACGAATGGTG ACCAGTCTGA TTATTTCGGT GCCAATGCTG CGGTTCATGG GTTATGCTTA 
CTCTCCCAGG TTCCCGATCA GCAGCAAAAG CTTCAGCAAC CCATCTCCTC TGAAAACGAT 
CAAGCAGCAT CCACCACTGC CAACAACCTC CTGAAGCAAA CTCAGCAACA GACTTTCCCC 
GACTCTATCC GGCCATCTTT TACTCAAAAT ACTAACCCCC AAGCTGTTAC AGGTACAATG 
AACCCTCAAG CATCTCGTAC TCAGCAACAG CCTATGTACT TTATGGGGTC GCAACAATTT 
AACGGCATGC CATCTGTTTA CGGCGATACA GTTAATCCTG CCGACCCTTC ATTAACATTA 
CGCCAAACCA CGGACTTTTC CGGACAAAAT GCCGAGAATG GATCCACAAA TCTACCGCAA 
AAAACTTCTA ATTCTGATAT GCCAACGGCA AATTCGATGC CGGTCAAACT CGAAAATGGT 
ACTGACTATT CCACTTCGCA AGAGCCTTCT AGTAATGCCA ATAATCAATC CTCACCCACT 
TCTTCCATTA ATGGTAAGGC TTCTAGTGAA TCTGCCAACG GCACTTCGTA TAGCAAAGGT 
TCATCTCGCC GGAATTCCAA AAACGAAACT GATGAAGAGA AACGTAAAAG CTTCCTGGAA 
CGCAATCGAC AAGCTGCTCT TAAATGTAGA CAGCGTAAAA AACAATGGCT TTCTAATCTT 
CAAGCGAAGG TTGAATTTTA TGGTAATGAA AATGAAATTT TAAGTGCACA AGTCAGTGCA 
TTACGTGAGG AAATTGTGAG TTTGAAGACC TTATTGATTG CTCATAAAGA CTGCCCAGTG 
GCCAAGAGTA ATTCGGCTGC TGTTGCAACA AGTGTCATCG GAAGCGGTGA TCTTGCTCAA 
AGAATCAATT TAGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595652.1
CDS	1468..3168
Translation	MSPSPVNTSTEPASVAAVSNGNATASSTQVPENNQSDSFAPPSNNSQQNQQSSTIAPNGGAGSVANANPADQSDGVTPSFVGSLKLDYEPNPFEHSFGSTASVGQGNPSLNRNPSLSNIPSGVPPAFARTLLPPVSSIASPDILSGAPGIASPLGYPAWSAFTRGTMHNPLSPAIYDATLRPDYLNNPSDASAAARFSSGTGFTPGVNEPFRSLLTPTGAGFPAPSPGTANLLGFHTFDSQFPDQYRFTPRDGKPPVVNGTNGDQSDYFGANAAVHGLCLLSQVPDQQQKLQQPISSENDQAASTTANNLLKQTQQQTFPDSIRPSFTQNTNPQAVTGTMNPQASRTQQQPMYFMGSQQFNGMPSVYGDTVNPADPSLTLRQTTDFSGQNAENGSTNLPQKTSNSDMPTANSMPVKLENGTDYSTSQEPSSNANNQSSPTSSINGKASSESANGTSYSKGSSRRNSKNETDEEKRKSFLERNRQAALKCRQRKKQWLSNLQAKVEFYGNENEILSAQVSALREEIVSLKTLLIAHKDCPVAKSNSAAVATSVIGSGDLAQRINLGY

Target ORF information:

RefSeq Version	NM_001021546.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- transcription factor, Atf-CREB family Atf1 (atf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021546.2

ORF Insert Sequence:

ATGTCCCCGT CTCCCGTCAA TACTTCCACA GAACCAGCAT CTGTTGCCGC TGTTTCAAAT 
GGTAATGCTA CTGCTTCCAG TACTCAGGTT CCGGAAAACA ATCAATCTGA TTCATTTGCA 
CCACCAAGCA ACAATTCTCA GCAGAATCAA CAGTCTTCAA CTATCGCTCC TAATGGAGGG 
GCCGGATCTG TCGCTAATGC GAATCCAGCT GACCAATCCG ATGGTGTAAC TCCGTCTTTC 
GTAGGCTCCT TGAAGCTGGA TTACGAACCC AACCCCTTTG AGCATTCATT TGGCTCTACC 
GCTTCGGTTG GACAAGGAAA TCCTTCTCTT AATCGTAATC CTAGCCTTAG TAACATTCCT 
TCAGGTGTGC CTCCTGCGTT TGCGCGAACT CTCTTACCTC CTGTTTCTTC CATAGCATCA 
CCTGATATCT TAAGTGGAGC TCCAGGTATT GCCTCGCCGT TGGGCTATCC TGCTTGGTCT 
GCTTTTACTC GTGGTACAAT GCATAATCCC CTTTCTCCTG CTATTTATGA TGCTACTCTT 
CGTCCTGACT ACCTAAACAA TCCTTCAGAT GCATCTGCAG CAGCTAGATT TTCTAGTGGA 
ACTGGATTTA CTCCTGGTGT TAATGAACCC TTCCGATCTC TTTTAACCCC TACTGGAGCT 
GGATTTCCCG CTCCTTCACC TGGTACTGCC AATTTATTAG GGTTTCATAC GTTTGATTCT 
CAATTTCCTG ATCAATATCG TTTTACCCCA AGGGATGGTA AACCGCCTGT TGTAAATGGG 
ACGAATGGTG ACCAGTCTGA TTATTTCGGT GCCAATGCTG CGGTTCATGG GTTATGCTTA 
CTCTCCCAGG TTCCCGATCA GCAGCAAAAG CTTCAGCAAC CCATCTCCTC TGAAAACGAT 
CAAGCAGCAT CCACCACTGC CAACAACCTC CTGAAGCAAA CTCAGCAACA GACTTTCCCC 
GACTCTATCC GGCCATCTTT TACTCAAAAT ACTAACCCCC AAGCTGTTAC AGGTACAATG 
AACCCTCAAG CATCTCGTAC TCAGCAACAG CCTATGTACT TTATGGGGTC GCAACAATTT 
AACGGCATGC CATCTGTTTA CGGCGATACA GTTAATCCTG CCGACCCTTC ATTAACATTA 
CGCCAAACCA CGGACTTTTC CGGACAAAAT GCCGAGAATG GATCCACAAA TCTACCGCAA 
AAAACTTCTA ATTCTGATAT GCCAACGGCA AATTCGATGC CGGTCAAACT CGAAAATGGT 
ACTGACTATT CCACTTCGCA AGAGCCTTCT AGTAATGCCA ATAATCAATC CTCACCCACT 
TCTTCCATTA ATGGTAAGGC TTCTAGTGAA TCTGCCAACG GCACTTCGTA TAGCAAAGGT 
TCATCTCGCC GGAATTCCAA AAACGAAACT GATGAAGAGA AACGTAAAAG CTTCCTGGAA 
CGCAATCGAC AAGCTGCTCT TAAATGTAGA CAGCGTAAAA AACAATGGCT TTCTAATCTT 
CAAGCGAAGG TTGAATTTTA TGGTAATGAA AATGAAATTT TAAGTGCACA AGTCAGTGCA 
TTACGTGAGG AAATTGTGAG TTTGAAGACC TTATTGATTG CTCATAAAGA CTGCCCAGTG 
GCCAAGAGTA ATTCGGCTGC TGTTGCAACA AGTGTCATCG GAAGCGGTGA TCTTGCTCAA 
AGAATCAATT TAGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atf1 ( NM_001356227.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342974.1 Schizosaccharomyces pombe Atf-CREB family transcription factor Atf1 (atf1), mRNA.

Price & Availability↑

CloneID	OSc100210
Clone ID Related Accession (Same CDS sequence)	NM_001356227.1
Accession Version	NM_001356227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Atf-CREB family transcription factor Atf1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

ATGTCCCCGT CTCCCGTCAA TACTTCCACA GAACCAGCAT CTGTTGCCGC TGTTTCAAAT 
GGTAATGCTA CTGCTTCCAG TACTCAGGTT CCGGAAAACA ATCAATCTGA TTCATTTGCA 
CCACCAAGCA ACAATTCTCA GCAGAATCAA CAGTCTTCAA CTATCGCTCC TAATGGAGGG 
GCCGGATCTG TCGCTAATGC GAATCCAGCT GACCAATCCG ATGGTGTAAC TCCGTCTTTC 
GTAGGCTCCT TGAAGCTGGA TTACGAACCC AACCCCTTTG AGCATTCATT TGGCTCTACC 
GCTTCGGTTG GACAAGGAAA TCCTTCTCTT AATCGTAATC CTAGCCTTAG TAACATTCCT 
TCAGGTGTGC CTCCTGCGTT TGCGCGAACT CTCTTACCTC CTGTTTCTTC CATAGCATCA 
CCTGATATCT TAAGTGGAGC TCCAGGTATT GCCTCGCCGT TGGGCTATCC TGCTTGGTCT 
GCTTTTACTC GTGGTACAAT GCATAATCCC CTTTCTCCTG CTATTTATGA TGCTACTCTT 
CGTCCTGACT ACCTAAACAA TCCTTCAGAT GCATCTGCAG CAGCTAGATT TTCTAGTGGA 
ACTGGATTTA CTCCTGGTGT TAATGAACCC TTCCGATCTC TTTTAACCCC TACTGGAGCT 
GGATTTCCCG CTCCTTCACC TGGTACTGCC AATTTATTAG GGTTTCATAC GTTTGATTCT 
CAATTTCCTG ATCAATATCG TTTTACCCCA AGGGATGGTA AACCGCCTGT TGTAAATGGG 
ACGAATGGTG ACCAGTCTGA TTATTTCGGT GCCAATGCTG CGGTTCATGG GTTATGCTTA 
CTCTCCCAGG TTCCCGATCA GCAGCAAAAG CTTCAGCAAC CCATCTCCTC TGAAAACGAT 
CAAGCAGCAT CCACCACTGC CAACAACCTC CTGAAGCAAA CTCAGCAACA GACTTTCCCC 
GACTCTATCC GGCCATCTTT TACTCAAAAT ACTAACCCCC AAGCTGTTAC AGGTACAATG 
AACCCTCAAG CATCTCGTAC TCAGCAACAG CCTATGTACT TTATGGGGTC GCAACAATTT 
AACGGCATGC CATCTGTTTA CGGCGATACA GTTAATCCTG CCGACCCTTC ATTAACATTA 
CGCCAAACCA CGGACTTTTC CGGACAAAAT GCCGAGAATG GATCCACAAA TCTACCGCAA 
AAAACTTCTA ATTCTGATAT GCCAACGGCA AATTCGATGC CGGTCAAACT CGAAAATGGT 
ACTGACTATT CCACTTCGCA AGAGCCTTCT AGTAATGCCA ATAATCAATC CTCACCCACT 
TCTTCCATTA ATGGTAAGGC TTCTAGTGAA TCTGCCAACG GCACTTCGTA TAGCAAAGGT 
TCATCTCGCC GGAATTCCAA AAACGAAACT GATGAAGAGA AACGTAAAAG CTTCCTGGAA 
CGCAATCGAC AAGCTGCTCT TAAATGTAGA CAGCGTAAAA AACAATGGCT TTCTAATCTT 
CAAGCGAAGG TTGAATTTTA TGGTAATGAA AATGAAATTT TAAGTGCACA AGTCAGTGCA 
TTACGTGAGG AAATTGTGAG TTTGAAGACC TTATTGATTG CTCATAAAGA CTGCCCAGTG 
GCCAAGAGTA ATTCGGCTGC TGTTGCAACA AGTGTCATCG GAAGCGGTGA TCTTGCTCAA 
AGAATCAATT TAGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342974.1
CDS	613..2313
Translation	MSPSPVNTSTEPASVAAVSNGNATASSTQVPENNQSDSFAPPSNNSQQNQQSSTIAPNGGAGSVANANPADQSDGVTPSFVGSLKLDYEPNPFEHSFGSTASVGQGNPSLNRNPSLSNIPSGVPPAFARTLLPPVSSIASPDILSGAPGIASPLGYPAWSAFTRGTMHNPLSPAIYDATLRPDYLNNPSDASAAARFSSGTGFTPGVNEPFRSLLTPTGAGFPAPSPGTANLLGFHTFDSQFPDQYRFTPRDGKPPVVNGTNGDQSDYFGANAAVHGLCLLSQVPDQQQKLQQPISSENDQAASTTANNLLKQTQQQTFPDSIRPSFTQNTNPQAVTGTMNPQASRTQQQPMYFMGSQQFNGMPSVYGDTVNPADPSLTLRQTTDFSGQNAENGSTNLPQKTSNSDMPTANSMPVKLENGTDYSTSQEPSSNANNQSSPTSSINGKASSESANGTSYSKGSSRRNSKNETDEEKRKSFLERNRQAALKCRQRKKQWLSNLQAKVEFYGNENEILSAQVSALREEIVSLKTLLIAHKDCPVAKSNSAAVATSVIGSGDLAQRINLGY

Target ORF information:

RefSeq Version	NM_001356227.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Atf-CREB family transcription factor Atf1 (atf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356227.1

ORF Insert Sequence:

ATGTCCCCGT CTCCCGTCAA TACTTCCACA GAACCAGCAT CTGTTGCCGC TGTTTCAAAT 
GGTAATGCTA CTGCTTCCAG TACTCAGGTT CCGGAAAACA ATCAATCTGA TTCATTTGCA 
CCACCAAGCA ACAATTCTCA GCAGAATCAA CAGTCTTCAA CTATCGCTCC TAATGGAGGG 
GCCGGATCTG TCGCTAATGC GAATCCAGCT GACCAATCCG ATGGTGTAAC TCCGTCTTTC 
GTAGGCTCCT TGAAGCTGGA TTACGAACCC AACCCCTTTG AGCATTCATT TGGCTCTACC 
GCTTCGGTTG GACAAGGAAA TCCTTCTCTT AATCGTAATC CTAGCCTTAG TAACATTCCT 
TCAGGTGTGC CTCCTGCGTT TGCGCGAACT CTCTTACCTC CTGTTTCTTC CATAGCATCA 
CCTGATATCT TAAGTGGAGC TCCAGGTATT GCCTCGCCGT TGGGCTATCC TGCTTGGTCT 
GCTTTTACTC GTGGTACAAT GCATAATCCC CTTTCTCCTG CTATTTATGA TGCTACTCTT 
CGTCCTGACT ACCTAAACAA TCCTTCAGAT GCATCTGCAG CAGCTAGATT TTCTAGTGGA 
ACTGGATTTA CTCCTGGTGT TAATGAACCC TTCCGATCTC TTTTAACCCC TACTGGAGCT 
GGATTTCCCG CTCCTTCACC TGGTACTGCC AATTTATTAG GGTTTCATAC GTTTGATTCT 
CAATTTCCTG ATCAATATCG TTTTACCCCA AGGGATGGTA AACCGCCTGT TGTAAATGGG 
ACGAATGGTG ACCAGTCTGA TTATTTCGGT GCCAATGCTG CGGTTCATGG GTTATGCTTA 
CTCTCCCAGG TTCCCGATCA GCAGCAAAAG CTTCAGCAAC CCATCTCCTC TGAAAACGAT 
CAAGCAGCAT CCACCACTGC CAACAACCTC CTGAAGCAAA CTCAGCAACA GACTTTCCCC 
GACTCTATCC GGCCATCTTT TACTCAAAAT ACTAACCCCC AAGCTGTTAC AGGTACAATG 
AACCCTCAAG CATCTCGTAC TCAGCAACAG CCTATGTACT TTATGGGGTC GCAACAATTT 
AACGGCATGC CATCTGTTTA CGGCGATACA GTTAATCCTG CCGACCCTTC ATTAACATTA 
CGCCAAACCA CGGACTTTTC CGGACAAAAT GCCGAGAATG GATCCACAAA TCTACCGCAA 
AAAACTTCTA ATTCTGATAT GCCAACGGCA AATTCGATGC CGGTCAAACT CGAAAATGGT 
ACTGACTATT CCACTTCGCA AGAGCCTTCT AGTAATGCCA ATAATCAATC CTCACCCACT 
TCTTCCATTA ATGGTAAGGC TTCTAGTGAA TCTGCCAACG GCACTTCGTA TAGCAAAGGT 
TCATCTCGCC GGAATTCCAA AAACGAAACT GATGAAGAGA AACGTAAAAG CTTCCTGGAA 
CGCAATCGAC AAGCTGCTCT TAAATGTAGA CAGCGTAAAA AACAATGGCT TTCTAATCTT 
CAAGCGAAGG TTGAATTTTA TGGTAATGAA AATGAAATTT TAAGTGCACA AGTCAGTGCA 
TTACGTGAGG AAATTGTGAG TTTGAAGACC TTATTGATTG CTCATAAAGA CTGCCCAGTG 
GCCAAGAGTA ATTCGGCTGC TGTTGCAACA AGTGTCATCG GAAGCGGTGA TCTTGCTCAA 
AGAATCAATT TAGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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atf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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