SPBC14F5.10c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC14F5.10c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC14F5.10c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100667 NM_001022662.2
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Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPBC14F5.10c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100667
    Clone ID Related Accession (Same CDS sequence) NM_001022662.2
    Accession Version NM_001022662.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative ubiquitin-protein ligase E3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022662.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGAAAAG AAAGGCCAGG AGTATTATTT AATAAAATAA GGTCTTACTT TATTTGTCCG 
    GGCTGTAATT GTTTGCCTGA CTGGCCTGTC ACACTTCCTT GTGGAGGAAC AGTCTGTCGG
    AAATGCTTTC GAAACGCATA TTCTTCGGAA AGCTCTGGAA AAGTTAGTCC CTCAAGATGT
    TGCTTTTACA ACCATAAGAA ACCGCATTAT TCTGTAGAGA CCGAAGTGAA AGATGTGATC
    ATTTCCAAAG TGGTTGAATT GATTAAAACT ACAGAGTTTC AAATTTCTCA GCAGAGTTTG
    GTACCGCTGG AATTAAAGGA GGAAATTTGT CATGACGATT GTCTTTCTTC GTCACCACCT
    TGTACTTCTG CATTGACTGA AATTACTCTG CTGCCTCCCA CTTTTCATAA CTTAATACCT
    TCCTCATCTT CTTATGAGAC TGCAGTAGCT GAATTTTTAC ATATGGAGGA CCTATTACAA
    GAGAATGTTT CTCGAGAATT GGAATGTCAA ATTTGTTTTG GCATGCTTTA TGACCCAGTT
    GTTTCTCCAT GTGGACATAC ATTTTGTGGC CCTTGCCTTA TGCAAGCACT AACCCAGTCT
    CCTCAATGCC CGACTTGTCG TTTTGGGCTC CCATCTCCTG TTGTGTTGGA ACATGCGAAA
    AGCCACTCTA TTACCACATT TTTGCGAGAT TTTTACCCAG ATAATTGGTT AGAACGTCAA
    AAATCATGGG AAGAAGAAAA GGAACAGGAA AGTTGGCTTC CGTTATTTAT TAGCATGCTT
    GCATATCCCA GAATGCCGAC ATTCCTTCAT ATTTTTGAAC TGCGATACCA CATCATGATC
    AAAAAATGTC TGGAGACTTC TAAGCGATTT TGTATTGCAA TGCCTTTAAG AGCACGAAGT
    GATGGTCATA ATGAGCATCG AGAGCTTCGA AATGCTCGTG GTCAACGTCT TTTTTGTTCT
    GAGTATGGTA CAATTTTAGA AATTATCCAA GTCGAGCCTT TGATCGATGG GAGAAGTCTT
    GTAGAAGCCC GTGGTTCATA TTGTGTTCGT ATAATCGATT TTCGAGCTGA TGGTTTGTTT
    CCGAGAGTGA AAATTGAAAA GCACTATGAT ACTCCTTTGA GAGCCACTCC ACTCCAATTT
    CCTGAGCCTG AATATCTATT AATGTATGGT AATCTAAGTA ATGAAGAATT AGTTGAAAGA
    ATAGATGCTT TTTACATGAA TGCCCGCCGA ACATATGTTC ATTGGGTTGT ACCTTTAATC
    GATATAAAAA TGGAGGCTAG ACAGTCAATT GCTGACTTGT CTTACAAAAT CACCAATCTT
    CTTCCTATCT CAGAGCTTGA GAAAACTCGC ATTTTGCAAG TTGACAACCC GACCGATCGA
    CTGGTTTTGG TTCTTATTTG GTTAACTCAA CTTCAAGAAA GTTGGTGGTA TCGTGTTGGA
    AGTGCTTGCA CCATTGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596736.1
    CDS346..1806
    Translation

    Target ORF information:

    RefSeq Version NM_001022662.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPBC14F5.10c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022662.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGAAAAG AAAGGCCAGG AGTATTATTT AATAAAATAA GGTCTTACTT TATTTGTCCG 
    GGCTGTAATT GTTTGCCTGA CTGGCCTGTC ACACTTCCTT GTGGAGGAAC AGTCTGTCGG
    AAATGCTTTC GAAACGCATA TTCTTCGGAA AGCTCTGGAA AAGTTAGTCC CTCAAGATGT
    TGCTTTTACA ACCATAAGAA ACCGCATTAT TCTGTAGAGA CCGAAGTGAA AGATGTGATC
    ATTTCCAAAG TGGTTGAATT GATTAAAACT ACAGAGTTTC AAATTTCTCA GCAGAGTTTG
    GTACCGCTGG AATTAAAGGA GGAAATTTGT CATGACGATT GTCTTTCTTC GTCACCACCT
    TGTACTTCTG CATTGACTGA AATTACTCTG CTGCCTCCCA CTTTTCATAA CTTAATACCT
    TCCTCATCTT CTTATGAGAC TGCAGTAGCT GAATTTTTAC ATATGGAGGA CCTATTACAA
    GAGAATGTTT CTCGAGAATT GGAATGTCAA ATTTGTTTTG GCATGCTTTA TGACCCAGTT
    GTTTCTCCAT GTGGACATAC ATTTTGTGGC CCTTGCCTTA TGCAAGCACT AACCCAGTCT
    CCTCAATGCC CGACTTGTCG TTTTGGGCTC CCATCTCCTG TTGTGTTGGA ACATGCGAAA
    AGCCACTCTA TTACCACATT TTTGCGAGAT TTTTACCCAG ATAATTGGTT AGAACGTCAA
    AAATCATGGG AAGAAGAAAA GGAACAGGAA AGTTGGCTTC CGTTATTTAT TAGCATGCTT
    GCATATCCCA GAATGCCGAC ATTCCTTCAT ATTTTTGAAC TGCGATACCA CATCATGATC
    AAAAAATGTC TGGAGACTTC TAAGCGATTT TGTATTGCAA TGCCTTTAAG AGCACGAAGT
    GATGGTCATA ATGAGCATCG AGAGCTTCGA AATGCTCGTG GTCAACGTCT TTTTTGTTCT
    GAGTATGGTA CAATTTTAGA AATTATCCAA GTCGAGCCTT TGATCGATGG GAGAAGTCTT
    GTAGAAGCCC GTGGTTCATA TTGTGTTCGT ATAATCGATT TTCGAGCTGA TGGTTTGTTT
    CCGAGAGTGA AAATTGAAAA GCACTATGAT ACTCCTTTGA GAGCCACTCC ACTCCAATTT
    CCTGAGCCTG AATATCTATT AATGTATGGT AATCTAAGTA ATGAAGAATT AGTTGAAAGA
    ATAGATGCTT TTTACATGAA TGCCCGCCGA ACATATGTTC ATTGGGTTGT ACCTTTAATC
    GATATAAAAA TGGAGGCTAG ACAGTCAATT GCTGACTTGT CTTACAAAAT CACCAATCTT
    CTTCCTATCT CAGAGCTTGA GAAAACTCGC ATTTTGCAAG TTGACAACCC GACCGATCGA
    CTGGTTTTGG TTCTTATTTG GTTAACTCAA CTTCAAGAAA GTTGGTGGTA TCGTGTTGGA
    AGTGCTTGCA CCATTGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L