cut1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	cut1
Entrez Gene ID	2539442
Full Name	separase/separin
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022895.2	NP_587903.1	separase/separin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cut1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cut1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99555	NM_001022895.2 Latest version!	Schizosaccharomyces pombe separase/separin (cut1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cut1 ( NM_001022895.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587903.1 Schizosaccharomyces pombe separase/separin (cut1), mRNA.

Price & Availability↑

CloneID	OSc99555
Clone ID Related Accession (Same CDS sequence)	NM_001022895.2
Accession Version	NM_001022895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	separase/separin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022895.1.

ATGTCTACAA GGTCAATCGT TACTAGTAAA GTTTCTTGGA CTCCAGAGAA GTTCATTTCA 
GCTCTCAGTT ATCCTGAGCA TTGCTCTATT ACTCTCGTTA AGCGACTCAA GGCTTCTGTA 
AAATTAAAGG ACCTCAAGCA AAATATATCT CGTGATGCAC CTTCGTGGAC GTTCGAGCAT 
TTGTTTGTGG CTTTCAAATG TGCAGTCAGT AACTTAGCTA AGCAATGGGC GGAGCTATCC 
ACTACAGATA AGGAGAAAAC TAGAAGGATG TTTTGTACAC CTTCTAGATT AAATACGGCT 
CACCGTCCCG AGGTTTTCTA CCTGTTAGAA TGCTGTACAT ATATTCTCGA ACAAATGCAG 
GTGGTAACCA AAAATACAAG TCATTTGTAT GATTGCATCC GTAGCGGGGT TTCAATTTGT 
AATCGGCTCC TGGACATGGA GATTTTTGAA CCAGCTATCA GTCTTTTGAT GAAAACTCAT 
AAAAACCTTA TAATTTTACT GACATATCGG GACCACGATG CCATCCCTAC TGCTACCCTT 
CTTAATCCCA CTTTAGATGT TTCAGAAATC CAATTAGAAA GCTGCCTTTT TGTTCCTATG 
GTTCCTGCCT CTTATTTCTT AAATATAGGC ACTATCGTTG TCACATTTCA GCTCAATGTA 
TTACGATGTT TATCTTTAAG TCAAATTAAC GGTTTGTCTT TGAATACAAT CAATAACTTA 
CAAAGCGAAG ATGGCCCTTT TCAATGGATT GAAAGAAGCT TTCCTTCCCA GGTTCAATTG 
GCCAATTCGA GAAGAGAAAT TTTAGCTCGT TTGTTGACAA GATTTTCCAT GATACAAAAT 
AACGCCTTAC AATCGTTCAA ACTTTTAATC CTCTCTATAG CTTTATGGTT GAATATTCTT 
TCTTCCCAGA GAGCTGACGA TAAAGAGTTT GATGTAAATC AGCTTGAAAC GAGAATCCTC 
CAACTTTTCT CAAAAGTTGT CCAATTATGT AAAAGCGAAG ATATTGAAGG ATCTATTTTA 
AATAAAGATA TGACACAACT TCATCATCTT CTGGAAAATT TATCAAAAGA ATCGAGGTTG 
CATATTTTGC TTCAACTTTC TCAACTTTAT TATAAATATA ATGATTTCCA ATTAAGCGCT 
GCATATGTCA TTAGAGGCTA TTCTTTGTCA TTTGAAGACA TCAGTTTTAA ATTAAAATTT 
TTATTATTTT CTTTTCGATT ATCAATTCAC GATAATAGCA TTTGTTTTCC GTTCAATTTA 
ATCCAAGAGT TGTCGTCACT TCAGCAGCTC TTTGTCGAAA ATGCTTTACC ATATTCAGAA 
GCTCTACATT TATTGGATTC AATAGAGCGA TCATTTCGAC TTTTCAATGA CTCGACGGTT 
TTTGATGATA CTGTATTTGC TTTGAACATA TCAGAAATTT TATCGTGGAT ACTCTCATCG 
GTTGTGAGGG ATATCCTTGT CGAGGATGAG CTTTTAAATC TCCAACTTAA AATAAGAAAG 
TTTTTGATGT TTACATTTCA TATAATTAGG TCATTTAGTG AGTTGACTAA ATTTCAAAGT 
TCTCTGGAAG GCTGTTTAAA TCTCGCAGCC TACTATGAAG ATGCTGAATT TCCTCAAAAG 
CTTTCCAATC ATCTTTACAA CTTGTGTGTA AAATCTTCTA ATGTTAATTA TGCACGTGAA 
TGCATAAGTT TGAGTATCAA AATTGCTGTA TCACATAAAT TGACTAATGA TGAAACATAT 
CTCCTGAAAA TTTTAAAAAA TTTTCAACTT CGGTACCACG ATTCTTTACA GTTGCAAGAA 
AAATGTGATG TACTCCATAC TACATTCAAT CAACTGGATC TCTATGTTGG AACTACATCT 
GTTGGAAAAT CTAGTGTATT GGATAACATT TTAAAACGTA TATTCAACTC TTTAACTTCT 
ATAAATGATT CAAATATAGA AAAATTGCTT GAATCAATAA GCTATTCCTT GCTCAAACTT 
TTTTTTAAAT GTGCAAATGA AGGTTCTCGA TATAATGCAT CTGCTGCTTT ATCTTTTAAA 
TTATCTCTTA TGTTACATGA AAAAGAGGAG GTCCTTTTGC TTAAAACAAA TGTTTCTTGC 
GTTTTGGCAA ACCATGGGTA TAATGACATT AAATTTGAGG AAATGGTTCT TTGCGTTATT 
AAAGGTGACC AAAATCTCCT AGAGCATAAT TCCAACAATA ATGCGAAATT AGCTTTAAAC 
GAGTCGCTAT TATGCTCATG GGAAAATTTG CTATGTTACA GACGTGCGGA AGACGATAGT 
AGGATACTTA CAATAATTGA GTCGTGGACT ATTTTTATAT CTCGATTTTC TTCGGTCATT 
TCTCGTTGCT CCTTCACAGA TTTTGAAATT AACTCAATTT TGAATTTTTT TTTTTGTTTT 
TTACATACGG TGGAGCCGAG TGGAAAACTA ACATTCGAGT TGGCTTTCCT TGAGATATTT 
TACGAACTTT TCAATTGTCT TCTTCATCTT CAATTTTCCA AGTATCTGGT CATCATTGGT 
ACTTTGCTTA GTGATAAATA TATGACGCTT GGCTTCTCAG GGAAAGCTCA CTTGTTTTAT 
ACCAAGTGTT ATAGCTATCT TCGTCAATGT AAAAGCAGTC CTTTTATAAA CTTTTGGAAT 
GTTTCTTATG GTAAATATTT GATTTTAACC GGAAATACGG ATAAAGGAAT TTTACAGTTA 
AAAAAATATT CTCTTTCTTC TGAAGAGGAT TTCAATTCAA ATGGTTTAAG TCGGACTGTT 
AGCCTCAATC TTTTGCTTTA TGAAAGGATA CAGCTAAGTG ATGCCCTATT TCAACTAGGC 
TACACCACTG TTTCTCTTGG CTTTATAATG CAAAATTTAA AGGTTATTAA GGGATTGTTT 
TCTAAAAGCT CAAAGGAGCA TTTCAACGGC GGAAAATATA TTACTTGGCG ACTTTTCGCT 
GTCAGTGCTC ACTCTAACGT TTGCGCTGCA AGGATTTATG AGCACATGGG ACAGGCGCGA 
GAAGCCGAAT TTTTTTATCG ACAAGCTTGC AGTATTTCTG AAAAGATGCC GTTTTCTTGT 
TTTTCAGCTA CCTTCCAATT GCGCTTATGC TCACTACTAA CAAGGGCAGG AAAGTTAGAG 
AAGGGCGAGA AGATTTTATT TGATCTTACT GAGGCGATGA AAAGCACAGA TACATACCAT 
AAACTTTTAT GGAATTACGG AGCAGCTGAA GTATGCGCAA CAAAATCCGA GTTGGATGGT 
GCCATATGTC ACTATTCAGA ATGTGTAAAA CTTTTAGAAA TAATAAAGTC AGAATATTAC 
CTTTTTTTTA ATAGGAACCG CGAGAAGTCT CTTACAAAAG GAATTAAAAG ACTCAGTCTT 
TCAAGTCAAC CAACTTTTGT TACAGAATCT AATACTACTG AATTTGATGA CTGGTCAATT 
CTTCAAAATA CGGCAGCCAA TTTACTTCGT CTGATTTCTA TGTTTGAGTT GAAAAGAGGA 
AACTTGGAGA TTGCAAAGGC TTTAATGACT GACTCGACAA AATGCTCAAT TGCAAGTTTT 
TTCAACATAG TCTCAGCTAA TATTTTAAAA TCAAAGCTTA TTGTTTGTGA AGCTGATTCC 
ACACTATTTG GCGATCCCGT CCTACGTACT CTTCCCGATT CTGTAATTTC ATTACCTGGA 
ATTTCTCATA AATTTCAGAA AAATCAAAGT AAAACAAAAG CGCTTGGAGA AAATACAGGA 
TTTCGCAAGG GATCCAAGCG CTTAGATTAT TTACGTGAGA GGCTTAAAAT CAATTTGCAG 
AATGTTAGAT TAAGCTGTGA AATTATATTT TCTAATGCAT ATGAGCGGAG CTCTGTATGC 
GTTTGCAGGG AGGTCAATGA ATTGATATCG TACTCAACAA TTATGCAAAG TGCATTAACC 
ACAATTGGCG AAACGACCGA CGTCGATTCT AGTTCAGCCT CATTTTTTTT AGAGATCCCT 
AAAGCTCTAG GATTCCATAG ACGTCGTGAA GCACAGAAAT TCAGAAACCA GCACAAAGAA 
CTTCATTTTA GTAGTTTGGA ACAAATTTTA AATAGTCGGT TATCTATACC TGATGTCCGT 
ACATTTCAAG ACAATTTTAT TGATTCATTA CCGTCGATCT GGAATGTCGT TTCTATTACT 
ATTAACAATT CTGGAGAGGA TTTGTTCATC TCAAAAATAC GCAAAGGTCA TTCTCCGCTG 
ATTTTTCGTT TACCATTGCA AAGACATAAT TCACGTGACG CAGACGAAGA AATCCTTGTG 
TTTACAAAAG CTCAAACCGA GCTATTTCGA ATTATCTCTA AAAGTAATCA GATGGCTCAG 
AACGGTAAAC ATTATACTAG AAGAGAAGAC AAAGAAACTT GGTGGAAAGA AAGAAGACAC 
CTTGATCAAT GCTTGCAACA ACTTTTAGAA AATATTGAAA TATCATGGCT CGGTGGATTT 
AAAGGTATTT TCAATCCACA TAAAATTGAT ACTTCTTTGT TTGCCAAGTT TTCCAGTCAA 
TTTCAAAACA TTATAGCTAA AAATTTTAAC ATGGACAAGA AAACACCTGT CCCTACACTG 
TCGCCTGAGA TCCTAGAACT ATTCATCACC TTAGGAAAAC CTGGGTATGA AGGTTACGAA 
CAGCTTTTAG AGGATTTGAT TTATTTTATT TTGGACATTT TTCAATTCCG AGGTCTTCAT 
TTTGCATACG ACGAGATTGA TACTGATCAA CTATCTATGG ATCTTCAGGA TGCTCTAAAT 
GCCTACTTCA ACAATTATGT CTCTGAAGAG AACAGGAGTC ATACGGTCCT TGTACTGGAT 
AAATCAGTTC ACCAATTTCC GTGGGAATCT TTACCATGCC TAAACCGACA ATCGGTATCA 
CGTGTGCCTT CATTATCAAT TCTGCGGGAC ATCTTGTCCC AATCATTTGT CGTTAATGGA 
GAGTATGTTG AAGTGCGTAA AGAAGCAGGT TCATATATAC TAAATCCTAG TTTGGACCTT 
AAGCATACTC AAGAAATGTT TGAACATAAA CTAGTTGAAG GTGGGTGGAA GGGATTAATT 
GCTAGTCAAC CTTCTAATAG AGATTTTATT AAAATGCTTT CAGGTAATGA TTTTTTTCTA 
TATTTCGGAC ATGGAGGAGG AGAGCAATAT ACAACATCTT ATGACCTGGC CACTCTAAAA 
AGGTGTGCAG TCACTATACT GATGGGTTGT AGTTCTGGTG CTTTGTATGA ATGTGGCTCA 
TTTGAGCCAT GGGGGACTCC TTTGGATTAT TTGAGTGCTG GGTGTCCAAC GTTAGTTGCA 
AACTTATGGG ATGTCACCGA TAAAGACATC GATAGGTTCA GTTTGAAGAT GCTTGAAAGC 
TGGGGACTCT TTGAAAATAA GGCACCTTTT GTGAATTCAA CGAGTATATG CACAGCGGTC 
AGCGAAAGTC GTTCATGCTG TCATCTACGC TATTTGAACG GAGCAGCTCC TGTGATATAC 
GGAATACCTG CTTATATTAT TCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_587903.1
CDS	466..5952
Translation	MSTRSIVTSKVSWTPEKFISALSYPEHCSITLVKRLKASVKLKDLKQNISRDAPSWTFEHLFVAFKCAVSNLAKQWAELSTTDKEKTRRMFCTPSRLNTAHRPEVFYLLECCTYILEQMQVVTKNTSHLYDCIRSGVSICNRLLDMEIFEPAISLLMKTHKNLIILLTYRDHDAIPTATLLNPTLDVSEIQLESCLFVPMVPASYFLNIGTIVVTFQLNVLRCLSLSQINGLSLNTINNLQSEDGPFQWIERSFPSQVQLANSRREILARLLTRFSMIQNNALQSFKLLILSIALWLNILSSQRADDKEFDVNQLETRILQLFSKVVQLCKSEDIEGSILNKDMTQLHHLLENLSKESRLHILLQLSQLYYKYNDFQLSAAYVIRGYSLSFEDISFKLKFLLFSFRLSIHDNSICFPFNLIQELSSLQQLFVENALPYSEALHLLDSIERSFRLFNDSTVFDDTVFALNISEILSWILSSVVRDILVEDELLNLQLKIRKFLMFTFHIIRSFSELTKFQSSLEGCLNLAAYYEDAEFPQKLSNHLYNLCVKSSNVNYARECISLSIKIAVSHKLTNDETYLLKILKNFQLRYHDSLQLQEKCDVLHTTFNQLDLYVGTTSVGKSSVLDNILKRIFNSLTSINDSNIEKLLESISYSLLKLFFKCANEGSRYNASAALSFKLSLMLHEKEEVLLLKTNVSCVLANHGYNDIKFEEMVLCVIKGDQNLLEHNSNNNAKLALNESLLCSWENLLCYRRAEDDSRILTIIESWTIFISRFSSVISRCSFTDFEINSILNFFFCFLHTVEPSGKLTFELAFLEIFYELFNCLLHLQFSKYLVIIGTLLSDKYMTLGFSGKAHLFYTKCYSYLRQCKSSPFINFWNVSYGKYLILTGNTDKGILQLKKYSLSSEEDFNSNGLSRTVSLNLLLYERIQLSDALFQLGYTTVSLGFIMQNLKVIKGLFSKSSKEHFNGGKYITWRLFAVSAHSNVCAARIYEHMGQAREAEFFYRQACSISEKMPFSCFSATFQLRLCSLLTRAGKLEKGEKILFDLTEAMKSTDTYHKLLWNYGAAEVCATKSELDGAICHYSECVKLLEIIKSEYYLFFNRNREKSLTKGIKRLSLSSQPTFVTESNTTEFDDWSILQNTAANLLRLISMFELKRGNLEIAKALMTDSTKCSIASFFNIVSANILKSKLIVCEADSTLFGDPVLRTLPDSVISLPGISHKFQKNQSKTKALGENTGFRKGSKRLDYLRERLKINLQNVRLSCEIIFSNAYERSSVCVCREVNELISYSTIMQSALTTIGETTDVDSSSASFFLEIPKALGFHRRREAQKFRNQHKELHFSSLEQILNSRLSIPDVRTFQDNFIDSLPSIWNVVSITINNSGEDLFISKIRKGHSPLIFRLPLQRHNSRDADEEILVFTKAQTELFRIISKSNQMAQNGKHYTRREDKETWWKERRHLDQCLQQLLENIEISWLGGFKGIFNPHKIDTSLFAKFSSQFQNIIAKNFNMDKKTPVPTLSPEILELFITLGKPGYEGYEQLLEDLIYFILDIFQFRGLHFAYDEIDTDQLSMDLQDALNAYFNNYVSEENRSHTVLVLDKSVHQFPWESLPCLNRQSVSRVPSLSILRDILSQSFVVNGEYVEVRKEAGSYILNPSLDLKHTQEMFEHKLVEGGWKGLIASQPSNRDFIKMLSGNDFFLYFGHGGGEQYTTSYDLATLKRCAVTILMGCSSGALYECGSFEPWGTPLDYLSAGCPTLVANLWDVTDKDIDRFSLKMLESWGLFENKAPFVNSTSICTAVSESRSCCHLRYLNGAAPVIYGIPAYIIP

Target ORF information:

RefSeq Version	NM_001022895.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe separase/separin (cut1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022895.2

ORF Insert Sequence:

ATGTCTACAA GGTCAATCGT TACTAGTAAA GTTTCTTGGA CTCCAGAGAA GTTCATTTCA 
GCTCTCAGTT ATCCTGAGCA TTGCTCTATT ACTCTCGTTA AGCGACTCAA GGCTTCTGTA 
AAATTAAAGG ACCTCAAGCA AAATATATCT CGTGATGCAC CTTCGTGGAC GTTCGAGCAT 
TTGTTTGTGG CTTTCAAATG TGCAGTCAGT AACTTAGCTA AGCAATGGGC GGAGCTATCC 
ACTACAGATA AGGAGAAAAC TAGAAGGATG TTTTGTACAC CTTCTAGATT AAATACGGCT 
CACCGTCCCG AGGTTTTCTA CCTGTTAGAA TGCTGTACAT ATATTCTCGA ACAAATGCAG 
GTGGTAACCA AAAATACAAG TCATTTGTAT GATTGCATCC GTAGCGGGGT TTCAATTTGT 
AATCGGCTCC TGGACATGGA GATTTTTGAA CCAGCTATCA GTCTTTTGAT GAAAACTCAT 
AAAAACCTTA TAATTTTACT GACATATCGG GACCACGATG CCATCCCTAC TGCTACCCTT 
CTTAATCCCA CTTTAGATGT TTCAGAAATC CAATTAGAAA GCTGCCTTTT TGTTCCTATG 
GTTCCTGCCT CTTATTTCTT AAATATAGGC ACTATCGTTG TCACATTTCA GCTCAATGTA 
TTACGATGTT TATCTTTAAG TCAAATTAAC GGTTTGTCTT TGAATACAAT CAATAACTTA 
CAAAGCGAAG ATGGCCCTTT TCAATGGATT GAAAGAAGCT TTCCTTCCCA GGTTCAATTG 
GCCAATTCGA GAAGAGAAAT TTTAGCTCGT TTGTTGACAA GATTTTCCAT GATACAAAAT 
AACGCCTTAC AATCGTTCAA ACTTTTAATC CTCTCTATAG CTTTATGGTT GAATATTCTT 
TCTTCCCAGA GAGCTGACGA TAAAGAGTTT GATGTAAATC AGCTTGAAAC GAGAATCCTC 
CAACTTTTCT CAAAAGTTGT CCAATTATGT AAAAGCGAAG ATATTGAAGG ATCTATTTTA 
AATAAAGATA TGACACAACT TCATCATCTT CTGGAAAATT TATCAAAAGA ATCGAGGTTG 
CATATTTTGC TTCAACTTTC TCAACTTTAT TATAAATATA ATGATTTCCA ATTAAGCGCT 
GCATATGTCA TTAGAGGCTA TTCTTTGTCA TTTGAAGACA TCAGTTTTAA ATTAAAATTT 
TTATTATTTT CTTTTCGATT ATCAATTCAC GATAATAGCA TTTGTTTTCC GTTCAATTTA 
ATCCAAGAGT TGTCGTCACT TCAGCAGCTC TTTGTCGAAA ATGCTTTACC ATATTCAGAA 
GCTCTACATT TATTGGATTC AATAGAGCGA TCATTTCGAC TTTTCAATGA CTCGACGGTT 
TTTGATGATA CTGTATTTGC TTTGAACATA TCAGAAATTT TATCGTGGAT ACTCTCATCG 
GTTGTGAGGG ATATCCTTGT CGAGGATGAG CTTTTAAATC TCCAACTTAA AATAAGAAAG 
TTTTTGATGT TTACATTTCA TATAATTAGG TCATTTAGTG AGTTGACTAA ATTTCAAAGT 
TCTCTGGAAG GCTGTTTAAA TCTCGCAGCC TACTATGAAG ATGCTGAATT TCCTCAAAAG 
CTTTCCAATC ATCTTTACAA CTTGTGTGTA AAATCTTCTA ATGTTAATTA TGCACGTGAA 
TGCATAAGTT TGAGTATCAA AATTGCTGTA TCACATAAAT TGACTAATGA TGAAACATAT 
CTCCTGAAAA TTTTAAAAAA TTTTCAACTT CGGTACCACG ATTCTTTACA GTTGCAAGAA 
AAATGTGATG TACTCCATAC TACATTCAAT CAACTGGATC TCTATGTTGG AACTACATCT 
GTTGGAAAAT CTAGTGTATT GGATAACATT TTAAAACGTA TATTCAACTC TTTAACTTCT 
ATAAATGATT CAAATATAGA AAAATTGCTT GAATCAATAA GCTATTCCTT GCTCAAACTT 
TTTTTTAAAT GTGCAAATGA AGGTTCTCGA TATAATGCAT CTGCTGCTTT ATCTTTTAAA 
TTATCTCTTA TGTTACATGA AAAAGAGGAG GTCCTTTTGC TTAAAACAAA TGTTTCTTGC 
GTTTTGGCAA ACCATGGGTA TAATGACATT AAATTTGAGG AAATGGTTCT TTGCGTTATT 
AAAGGTGACC AAAATCTCCT AGAGCATAAT TCCAACAATA ATGCGAAATT AGCTTTAAAC 
GAGTCGCTAT TATGCTCATG GGAAAATTTG CTATGTTACA GACGTGCGGA AGACGATAGT 
AGGATACTTA CAATAATTGA GTCGTGGACT ATTTTTATAT CTCGATTTTC TTCGGTCATT 
TCTCGTTGCT CCTTCACAGA TTTTGAAATT AACTCAATTT TGAATTTTTT TTTTTGTTTT 
TTACATACGG TGGAGCCGAG TGGAAAACTA ACATTCGAGT TGGCTTTCCT TGAGATATTT 
TACGAACTTT TCAATTGTCT TCTTCATCTT CAATTTTCCA AGTATCTGGT CATCATTGGT 
ACTTTGCTTA GTGATAAATA TATGACGCTT GGCTTCTCAG GGAAAGCTCA CTTGTTTTAT 
ACCAAGTGTT ATAGCTATCT TCGTCAATGT AAAAGCAGTC CTTTTATAAA CTTTTGGAAT 
GTTTCTTATG GTAAATATTT GATTTTAACC GGAAATACGG ATAAAGGAAT TTTACAGTTA 
AAAAAATATT CTCTTTCTTC TGAAGAGGAT TTCAATTCAA ATGGTTTAAG TCGGACTGTT 
AGCCTCAATC TTTTGCTTTA TGAAAGGATA CAGCTAAGTG ATGCCCTATT TCAACTAGGC 
TACACCACTG TTTCTCTTGG CTTTATAATG CAAAATTTAA AGGTTATTAA GGGATTGTTT 
TCTAAAAGCT CAAAGGAGCA TTTCAACGGC GGAAAATATA TTACTTGGCG ACTTTTCGCT 
GTCAGTGCTC ACTCTAACGT TTGCGCTGCA AGGATTTATG AGCACATGGG ACAGGCGCGA 
GAAGCCGAAT TTTTTTATCG ACAAGCTTGC AGTATTTCTG AAAAGATGCC GTTTTCTTGT 
TTTTCAGCTA CCTTCCAATT GCGCTTATGC TCACTACTAA CAAGGGCAGG AAAGTTAGAG 
AAGGGCGAGA AGATTTTATT TGATCTTACT GAGGCGATGA AAAGCACAGA TACATACCAT 
AAACTTTTAT GGAATTACGG AGCAGCTGAA GTATGCGCAA CAAAATCCGA GTTGGATGGT 
GCCATATGTC ACTATTCAGA ATGTGTAAAA CTTTTAGAAA TAATAAAGTC AGAATATTAC 
CTTTTTTTTA ATAGGAACCG CGAGAAGTCT CTTACAAAAG GAATTAAAAG ACTCAGTCTT 
TCAAGTCAAC CAACTTTTGT TACAGAATCT AATACTACTG AATTTGATGA CTGGTCAATT 
CTTCAAAATA CGGCAGCCAA TTTACTTCGT CTGATTTCTA TGTTTGAGTT GAAAAGAGGA 
AACTTGGAGA TTGCAAAGGC TTTAATGACT GACTCGACAA AATGCTCAAT TGCAAGTTTT 
TTCAACATAG TCTCAGCTAA TATTTTAAAA TCAAAGCTTA TTGTTTGTGA AGCTGATTCC 
ACACTATTTG GCGATCCCGT CCTACGTACT CTTCCCGATT CTGTAATTTC ATTACCTGGA 
ATTTCTCATA AATTTCAGAA AAATCAAAGT AAAACAAAAG CGCTTGGAGA AAATACAGGA 
TTTCGCAAGG GATCCAAGCG CTTAGATTAT TTACGTGAGA GGCTTAAAAT CAATTTGCAG 
AATGTTAGAT TAAGCTGTGA AATTATATTT TCTAATGCAT ATGAGCGGAG CTCTGTATGC 
GTTTGCAGGG AGGTCAATGA ATTGATATCG TACTCAACAA TTATGCAAAG TGCATTAACC 
ACAATTGGCG AAACGACCGA CGTCGATTCT AGTTCAGCCT CATTTTTTTT AGAGATCCCT 
AAAGCTCTAG GATTCCATAG ACGTCGTGAA GCACAGAAAT TCAGAAACCA GCACAAAGAA 
CTTCATTTTA GTAGTTTGGA ACAAATTTTA AATAGTCGGT TATCTATACC TGATGTCCGT 
ACATTTCAAG ACAATTTTAT TGATTCATTA CCGTCGATCT GGAATGTCGT TTCTATTACT 
ATTAACAATT CTGGAGAGGA TTTGTTCATC TCAAAAATAC GCAAAGGTCA TTCTCCGCTG 
ATTTTTCGTT TACCATTGCA AAGACATAAT TCACGTGACG CAGACGAAGA AATCCTTGTG 
TTTACAAAAG CTCAAACCGA GCTATTTCGA ATTATCTCTA AAAGTAATCA GATGGCTCAG 
AACGGTAAAC ATTATACTAG AAGAGAAGAC AAAGAAACTT GGTGGAAAGA AAGAAGACAC 
CTTGATCAAT GCTTGCAACA ACTTTTAGAA AATATTGAAA TATCATGGCT CGGTGGATTT 
AAAGGTATTT TCAATCCACA TAAAATTGAT ACTTCTTTGT TTGCCAAGTT TTCCAGTCAA 
TTTCAAAACA TTATAGCTAA AAATTTTAAC ATGGACAAGA AAACACCTGT CCCTACACTG 
TCGCCTGAGA TCCTAGAACT ATTCATCACC TTAGGAAAAC CTGGGTATGA AGGTTACGAA 
CAGCTTTTAG AGGATTTGAT TTATTTTATT TTGGACATTT TTCAATTCCG AGGTCTTCAT 
TTTGCATACG ACGAGATTGA TACTGATCAA CTATCTATGG ATCTTCAGGA TGCTCTAAAT 
GCCTACTTCA ACAATTATGT CTCTGAAGAG AACAGGAGTC ATACGGTCCT TGTACTGGAT 
AAATCAGTTC ACCAATTTCC GTGGGAATCT TTACCATGCC TAAACCGACA ATCGGTATCA 
CGTGTGCCTT CATTATCAAT TCTGCGGGAC ATCTTGTCCC AATCATTTGT CGTTAATGGA 
GAGTATGTTG AAGTGCGTAA AGAAGCAGGT TCATATATAC TAAATCCTAG TTTGGACCTT 
AAGCATACTC AAGAAATGTT TGAACATAAA CTAGTTGAAG GTGGGTGGAA GGGATTAATT 
GCTAGTCAAC CTTCTAATAG AGATTTTATT AAAATGCTTT CAGGTAATGA TTTTTTTCTA 
TATTTCGGAC ATGGAGGAGG AGAGCAATAT ACAACATCTT ATGACCTGGC CACTCTAAAA 
AGGTGTGCAG TCACTATACT GATGGGTTGT AGTTCTGGTG CTTTGTATGA ATGTGGCTCA 
TTTGAGCCAT GGGGGACTCC TTTGGATTAT TTGAGTGCTG GGTGTCCAAC GTTAGTTGCA 
AACTTATGGG ATGTCACCGA TAAAGACATC GATAGGTTCA GTTTGAAGAT GCTTGAAAGC 
TGGGGACTCT TTGAAAATAA GGCACCTTTT GTGAATTCAA CGAGTATATG CACAGCGGTC 
AGCGAAAGTC GTTCATGCTG TCATCTACGC TATTTGAACG GAGCAGCTCC TGTGATATAC 
GGAATACCTG CTTATATTAT TCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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cut1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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