mak3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mak3
Entrez Gene ID	2539117
Full Name	histidine kinase Mak3
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023370.2	NP_588379.1	histidine kinase Mak3

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mak3	NP_588379.1
Schizosaccharomyces pombe (fission yeast)	mak2	NP_594410.1
Magnaporthe oryzae (rice blast fungus)	MGG_06696	XP_003709405.1
Neurospora crassa	NCU01823	XP_955920.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate distinct functions for the three histidine kinases; Mak1 alone or Mak2 and Mak3 together were sufficient for the repression of the meiotic cycle when nutrients were available.
Title: Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.

The following mak3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mak3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99403	NM_001023370.2 Latest version!	Schizosaccharomyces pombe histidine kinase Mak3 (mak3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mak3 ( NM_001023370.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588379.1 Schizosaccharomyces pombe histidine kinase Mak3 (mak3), mRNA.

Price & Availability↑

CloneID	OSc99403
Clone ID Related Accession (Same CDS sequence)	NM_001023370.2
Accession Version	NM_001023370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	histidine kinase Mak3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023370.1.

ATGTATTCTC AGCATGAACT TCGTAATAAA GTCAGCCTAG CACTCTCGAG TCTACTTAGG 
TACACGTTTG AATTGACGCC TTTTTTTGAA CTGTACGAAG CTGATTTCGC ATATGCTTTG 
TATGCTGGCT TTGAACTGGC CACAAATCGA AAGGTGGTTG GAAAGTTCTC ATTTCAAAAT 
GTTCATCTTG AAAACGAGTA TAACATACTT ACGGAAATTG CAAAAGATGA AAGGGCATCG 
AAATTTAGCC CTACTCCTAT TGAGTTTACC TCTTTCCCCC ATATTGATTT ATCTGCTTGT 
ATTGCTTATG ACTTTGGCCA CGGAGCTGAA TTATCGACAA GTTATGCCTA TTTTAGAGAG 
AACCCAGCAG AATTTGTTCG CTTTTGTATT GCAATTTGTA AATGCATTGA ATACTTGCAC 
TCAAAAGGAA TGGTACATGG AGAAATACGT CTGGATAGTT TCATCCCCAT AAGCTCTTAC 
GACAATGTTT ACATGCTCAC TGTGGGATCA GGCGCTAGCT ATTTTCATAA TTGTTTACAA 
GCGCATAATT GGCGTAAATA TTCCGAAGAC TCAGAATCGA TGTCCAGAAT TTTGTTTATC 
AGTCCCGAGC AAACGGGCAG AACTTCATAC AGTGTCGGAT ATCGTACAGA TATATATAGT 
TTAGGGGTTT TATTTTTCCA TTACCTTTCA GATTGTTCTC CTTATACGGG ATCTTTTGTA 
CAACGAATTC GATCTATTTT GACAGAACCT TTACCCGACA TCAGTAAATC ATGTCCCAAA 
CTTCCGCATT TGATTTTTAA AATTATTGAA AAAATGACAC GAAAAAACCC AGATGAAAGA 
TACACTTCCT GTTCTGGTAT CGTTAACGAC TTGGAAGCTT GCTTGGATGA TATTGACAAA 
GGGTTAATAC TCAATGATCA TGTTTTGGAA AAAACAGGAC GTACATCTTT ATTCTATTTG 
CCTTGTTCTA TATATGGTCG TGAACATGAA ATCAAATTAA TCAGAAAAAT CTTAAGAAAT 
TCCCCGCGCG CAATAAATCA CCAAGACAAA AAGGATTTGG AGACATTTAA TCCATATTAT 
TTAAATGCGA TAGAATCTGA GAGCTCTTCT CAATCCCTCT CTTTATCCCA AAGGGCTTCT 
GAAGTTATGC CACTGGTAAT ACTTATCACA GGATGTGAGG GTATTGGAGA ATCAAGCTTG 
ATTCAAACTA TTTGTGATCG ACGTGAAGGG TATATGGCTA TCACAAAATT TGAAGTATCA 
CAATCAATTG TATACTCTGC GATTGTTTCA GCTGTTGCCG AGTTTATTCG GCAAATCCTT 
GCTGAAGATC AGCTTTTACT TAATAATTTT TTTGAAGAGC TTAAGAATAA ATTAGAATCG 
GATTTGTATT TGCTCGATTC GGTTTTCGAT TTGGTACCAG AAATTAGAAG TTTATTACAA 
CAGTTTTCGA CTTCTTCTGG TAATACTAGA AAAACGTCTT TGTTGGGCTC GAATCATTCT 
AGCTATTCCG ATAAACTTGG GTCTCCTACA ATTCTCTCAA CTTCGTTTTC ACTTGCAAGG 
CCATATCCTG AGCCGGCTCT TGTAAGTCCT TCGACTGAAA GGCCCCCTAG GTCAAGTTTT 
TCTGCCGCCT TGATGACCCT GCTAAATATC ATTGCTAGTT TTAAAAAAGT AACGATGGTT 
ATAGAGAATA TTCATCTTGC TGATGAGTCT TCTTTAATTA TTCTCCAGAA AATCGTTTAC 
TCTGATCTTC CACTTACCTT GATGATTACT TGCGATAAAG AAAACGATCA TGTAATTAAC 
AGGTTTCGTT TAGCGAATGA CAGGATACAC GAAATCGAGT TAAAACCACT GTCTTTTAAT 
GCTGTGAATT CCTATGTTCA GGCTACTTTG CATCGAACCG ATGATGGATT AGCAAGATTT 
TCTTCTTACG TTTATCACAT TAGCAAAGGT GTGCCTTTAC TTGTGAGAAA TGTACTACTG 
AGTATTTATG AAAACAAGAT AATTTACTTT GATTGGAAAA AAAATCGATG GGAAGTAAAT 
TACGACGAAA TGTACACTCT TGACAATGAT TATTCTGAGC CCGATGCATT TATGACGGCC 
AAGAAAAAAA TCAGTAAACT GAACGACTCT TCTCGTGCTA TCCTTGGTTG GGCTAGTCTT 
TTGGGCCCAT CTTTTTCTTT TGCAACTGTA AAGAAGCTTT GTAAGGATAC CGATAATATT 
GAATTAAATG TGGAGGCTCT TCAGTCCGCA TTAAGAGAAG GTATAATTTA CGCCACTTCT 
TCTGATGACA CGTATACGTT TTCAAGGTCT ATTTATGTTA AAGCGATGCG TGATTTGCTT 
AATGAAGCAA AAATACAGAT TATGCATGCA TGTCTTATTG ACGTTTGTCT TAAAAATCGA 
GATCGTTATA ACATCTTCGA TATCGCTTTT CATATCAATG CCGCTTTTGA TTTTGTTAAG 
GGTGATAAAC GATCTGTTGA ATATTGCCAT TATTTGCACT TGGCTGCCGA AGAGGCTTTA 
AAGATTGGAG CTAATCAAGA GGCGCTTGAC TTATATAACA GATGTATAAA AATGATACCA 
CACGAAATTC CTGAGGAAAG TGATGATAGT TATATTCGCT GCCAGCTTAT TGGTATGTAT 
GTTGGATGCG CTGAAGCTTA TTGGGTAAAT GATAATTTCG ATACAGCTTC AGAAATGTTA 
AAACTAGCAG AGGAGAAAGC TTGTAATAAC TCGGAGGTTT TTCCTGCAAG GTTTTTGTAC 
TCTCGGATTT TATTCGAAGG GGTGCATATA GAAGAGTGCA CTCAATATGT ATTATCTTGT 
TTGAAGCCAC TTGGGTATGA GTTAAAGCGA CATTCTCTCG AAGATTCAAA GTCAATTATT 
TCAGCACTGA TCCCACGTAT TATTGACAAA ATTACTAAAA GCTCAGAGGA ATCTCAGTCA 
TCAACAGATG ACGATGACCG GAGAATTTTC GAAATACTTT CTTTTTTATA CGTCGGTTCA 
GTCGCTACTT CTTACTTTTC AGAGACCGCA GAAATGGCCA TTGATTTTGG AATAGCACAA 
GTCGAATTTT TTTTAAGTAC TGTTGTCAAT TCGTTCTCCG CTTTTGCCTT AGTTTATTTT 
GCTATTCTTG CAAATTCTTT ACTTGAGCCT TCAGAAGATA TTCTCTTCAT CGGTAATTAT 
GGAGAGAAAC TGAATCGTGA AGCTGAGAAT CCTATAATAT TTTCACGTAC TGAATATTTG 
TATGTTCAAT CTCTTGGTTT TATAGACAGT ACCACGAAAG AGAGAAGACT TACTATTGAT 
TATTTGGACA GAAATTGTGT CACTTGCAGT GATAAACACG TTATTATTAG TCTGCTTTTA 
GTGTCATCAT GGGAGAAATT TCTAACTTCC AACAACTATT CAAATTACTT GGCAGATTTT 
GAAACTACTC ATGCGCAAAT TATGGAAATG AAGCCTTGGG TTGGTGATAC CTCATTAATA 
ACACAATTAA AGCGATTTTT GATGTGCTTA CAGGATAACA TCAAATTGGA TTTAATCAAG 
TCAAAGAGTT TTTTGTCGGA TCATAATATC CAATTATCAT CCCCAGCAGC ACAAGAATCT 
GCGAAACTTG CATTCAGCCT TCACGGATGG ATTAACTCAT GGTATCTTCT GGCTCTTGTG 
ATGCATGGTG AATGGGATAT GGCTATCAGT TATGGAGAGA ATTTTAAACG TGAATTTAAA 
AATGCGCTTT TAACTTCTTC TAGGGTATTT GGAATTTTTA TGTTTACTTG GTCTTTGGTC 
AACAAGATGC TCATTTGTCC CGAATTCACT AAGCAAAAAA AATATTATGA GCAGTATAAA 
GAAAATCTTG GATTTTTTGA TAGCCTATGC ATTGGTGATA ACGAATGTAT CACTCGTGTA 
TATTTTCTTT TATTAAAAGC ATGTGGTTTA ATAATGAATG GGCTGAATTT TGAAGCATCA 
GTTATGCTGG AGGAAGTCAT CTCTTTAACA GAAAAACTTG AACTTTTTTT GTTACAGGCA 
TTTGCATTTG AAACTGTTGG AAGCATTTTT GTGTCTATGG AACTATATAC TTCTGCTACT 
CAATACTTGG AAGAGGCTAT TCGAAATTAT GCTGCTCTGG GTGTTAAACA AAAAGCTAGG 
CATTTGAGGG ATAAGTTCGG TGATTTGTTG GTTTCGAACA ACTTACAGGT TTCGATTGAT 
GAAGCTACAC AAACAGATTT CCCTTTGGTG TTTAGTCCTG AGCGCTCAAG TATTGACATA 
AATGCTAGTA GTATGCGTTC TGAAAAAGCG TCCTTTGAGA TTCCTTTTCC TGAAGAGCAG 
ATTGATGATG ATGTTTCTCC AGTAGCCCAA GATTCTTCTC TGGAAGAGTT ACTTATATCT 
TTGGACATCA TCGATCTAAC CTCAGTAATG AGATCCTGCC AAACGATTGC CAGTGAAATT 
GAGTTGACTG GTTTGCTCTC GACTATGACA CAGAGAATGT TGGAAGATTC TTCAGCTAAC 
GCTGCTGTTA TAGCAATTCG TGATGACGTT GGCTTTAAAA TTGCAGCTTA TCGTACGGGA 
GAGCTTAACG AAGTTTTTGC TCCCCCGATG CCTATTACAG AAGATCAAAC GTACGTTCCT 
TCTAGAGTGA TAAATTATGT TGTCCATACG CAAAAAGCTT TGTTTTCGAA TAATATAAAC 
CATGAATTTG ATTTGCAGCA GGAGCGTTGG AATATCGAAA ATCATATGGG GAGAAGCGTA 
ATTGCTATTC CTTTATACCA AAAGAAGGAG GTTTTTGCGA TACTCTACTT GCAAGGCCCT 
CCATCAGCAT TTCATTCTCG ACATATGTCG GTACTATCAA TCCTTGGGGC TCAGGCAAGT 
TTCGCAATTG TGAATATATC TTTGTTTCAT AAGGTGAAAG AGGCAACTAA TGTTAATACG 
ATTATCATTA AAGCCCAGAG AGAAGCATTA AATTTGGTGC AAAAATCGGA GGCTAAATAT 
CGCAGCTTTG TCGATACAAT GCCTTGCTTG TTATCAAAAT TAGAATTTGA TGAAGAGTTA 
AGGATTGAGC TTTTTGGAAG TTTTTGGAAA GAATATTGTG GTGAATTAAA TATAAACGAC 
CCAAATACAT GGAAGGAATA TGTTCATCTT GACGATCACC TTAAATTACA GGATTTCCTG 
CTCTCTCACT TGCACAATCC TCTTCCTTTT GAACTAGAAA TAAGAATTAA AAGGAAGGAT 
GGAGTTTATC GATGGAATCT TACACGCTGT ACCCCTACGA CGAACGAAAA AAATAGAACT 
AGTTTTTTGT GTGCAACAAT TGATATTGAC GATCAAAAGA AGGCACGAGC TACCGCATTA 
GAACTGGCAC GTTTGCGTTC GAATTTCTTG GCGAACATTT CACACGAATT AAGAACACCT 
TTTTCTGGCT TCTACGGCAT GCTTTCTCTC TTAGATGATA CAAATTTAGA TTCTGAGCAA 
AGGGATATTG TTAGTGCTGC TCGTATAAGC TGTGAAATGC TTCTTCGGGT AATCAACGAT 
TTGTTGAATT TTAGCAAACT TGAAGCGGGC AAAGTCACTT TAGAATCTGA CCTTGAATTT 
TCTTTAGAAT CTGTCGTTTG TGATTGTATG CAATCTGTAT ATTCAGCTTG TGCCGAGAAA 
GGTATCAATT TATCTTATAA TGTTTCTCCA GATATTCCTT TTTTCACAGC GGGAGACGGC 
ATGAAAATTG GACAAATGTT AAAGAGTATC CTTGATAATT CGGTAAAAAC AGTTAACAAT 
GGATTTATCC GTGTTAGGGC CTTTTTGGCT GGTTCATCGA AAAAGAATGA TAGGGACCAG 
TTACAAATTG CGTTTATTGT AGAGGATACT CGCGAAGAAA GCAATGCTAT TTTTTTGGCT 
AATATGATCA ATTCCTTGAA TCGTGGCTGT AACGACTATT TACCCATGGA TTTAAGTGGT 
ACCGCACTTG GAATGTCCAC GTGTTTACAA CTTTGCAAAA TAATGGGTGG ATCAGTAAGT 
GTAGAGGTAT CACAAAATAA CCCTACATTT AAAATTTGTT ATGATCTGAA AATTCATGAA 
CTTGGAAAGG AAAGATACGA CATTATAGCT ACTCCTCTAT TTCAAAACCT AACAGAGTTC 
AATGATCTCA TAAAATCAAA AGTTGCTATC CGAGTTTCTA AAACTTCTAC TGAGTATGAC 
AACATTACTA CATATCTTCA AGCTGCGAGA AAGGTTTTGC ATGTTTTTAA GGGATTACAA 
GACCTAGCAT CAATTTTTGA CTTAAGCCCT GACTCTGCAC TTCTCCGCTG TTCCGTTGTG 
GTAGTGGATG TTTATTCGAT GGATGATGTT AAGGCAGTCG AAAAAATATT GAAAAGCTAT 
CCGGATGTAC ATGTCATATA TTTGTGCTGT GATCCCTCTA GATTGAACAT CGAGCAGGAA 
CTACAGAAAC CTTCAGGAAG ATCGTTTGCA TGTAAAAAAA GATGGGGATT TCTTCAAATG 
CCTTGTACTA GAGAAAACTT CCTCAAGGTT ACATTACAAG TGTTTAAGTC TAATGAAGAT 
ACTTGTAACT TTTACTCTTA TGTTAATGAG TACGGTGAAT CCCCAAAACC AGATGACGAT 
ATGGACCGGT TAAACAAATG TGTTGGATCA AAGATTTTAA TTGCTGAAGA CAACCCCATA 
GTGCGTATGA CTTTAAAAAA GCAACTAGAG CATTTAGGAA TGGATGTTGA TGCCGCAGAA 
GATGGAAAGG AAACTCTTCA AATTTTTGAG AGTCACCCCG ACAACTATTA CCAAGTTTGT 
TTTGTTGATT ATCATATGCC TGTATATGAT GGCTTAGAGG TAACCAGAAG GATGAGAAAG 
ATAGAGCGTA AGCATGGTTG TGCACCTCTT CCCATCTTTG CTTTGACCGC CGATATGCAG 
CCTACCATGG AAACTCAGTT TCAAGAAGTT GGAATAACGC ATTATCTCAG TAAACCTTTC 
AAGAAAGAAA CACTAATTAA AATGCTTCTG CAATATTTAG TTAACGGAAC TGATGGAAAT 
GCTAATACTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588379.1
CDS	114..7148
Translation	MYSQHELRNKVSLALSSLLRYTFELTPFFELYEADFAYALYAGFELATNRKVVGKFSFQNVHLENEYNILTEIAKDERASKFSPTPIEFTSFPHIDLSACIAYDFGHGAELSTSYAYFRENPAEFVRFCIAICKCIEYLHSKGMVHGEIRLDSFIPISSYDNVYMLTVGSGASYFHNCLQAHNWRKYSEDSESMSRILFISPEQTGRTSYSVGYRTDIYSLGVLFFHYLSDCSPYTGSFVQRIRSILTEPLPDISKSCPKLPHLIFKIIEKMTRKNPDERYTSCSGIVNDLEACLDDIDKGLILNDHVLEKTGRTSLFYLPCSIYGREHEIKLIRKILRNSPRAINHQDKKDLETFNPYYLNAIESESSSQSLSLSQRASEVMPLVILITGCEGIGESSLIQTICDRREGYMAITKFEVSQSIVYSAIVSAVAEFIRQILAEDQLLLNNFFEELKNKLESDLYLLDSVFDLVPEIRSLLQQFSTSSGNTRKTSLLGSNHSSYSDKLGSPTILSTSFSLARPYPEPALVSPSTERPPRSSFSAALMTLLNIIASFKKVTMVIENIHLADESSLIILQKIVYSDLPLTLMITCDKENDHVINRFRLANDRIHEIELKPLSFNAVNSYVQATLHRTDDGLARFSSYVYHISKGVPLLVRNVLLSIYENKIIYFDWKKNRWEVNYDEMYTLDNDYSEPDAFMTAKKKISKLNDSSRAILGWASLLGPSFSFATVKKLCKDTDNIELNVEALQSALREGIIYATSSDDTYTFSRSIYVKAMRDLLNEAKIQIMHACLIDVCLKNRDRYNIFDIAFHINAAFDFVKGDKRSVEYCHYLHLAAEEALKIGANQEALDLYNRCIKMIPHEIPEESDDSYIRCQLIGMYVGCAEAYWVNDNFDTASEMLKLAEEKACNNSEVFPARFLYSRILFEGVHIEECTQYVLSCLKPLGYELKRHSLEDSKSIISALIPRIIDKITKSSEESQSSTDDDDRRIFEILSFLYVGSVATSYFSETAEMAIDFGIAQVEFFLSTVVNSFSAFALVYFAILANSLLEPSEDILFIGNYGEKLNREAENPIIFSRTEYLYVQSLGFIDSTTKERRLTIDYLDRNCVTCSDKHVIISLLLVSSWEKFLTSNNYSNYLADFETTHAQIMEMKPWVGDTSLITQLKRFLMCLQDNIKLDLIKSKSFLSDHNIQLSSPAAQESAKLAFSLHGWINSWYLLALVMHGEWDMAISYGENFKREFKNALLTSSRVFGIFMFTWSLVNKMLICPEFTKQKKYYEQYKENLGFFDSLCIGDNECITRVYFLLLKACGLIMNGLNFEASVMLEEVISLTEKLELFLLQAFAFETVGSIFVSMELYTSATQYLEEAIRNYAALGVKQKARHLRDKFGDLLVSNNLQVSIDEATQTDFPLVFSPERSSIDINASSMRSEKASFEIPFPEEQIDDDVSPVAQDSSLEELLISLDIIDLTSVMRSCQTIASEIELTGLLSTMTQRMLEDSSANAAVIAIRDDVGFKIAAYRTGELNEVFAPPMPITEDQTYVPSRVINYVVHTQKALFSNNINHEFDLQQERWNIENHMGRSVIAIPLYQKKEVFAILYLQGPPSAFHSRHMSVLSILGAQASFAIVNISLFHKVKEATNVNTIIIKAQREALNLVQKSEAKYRSFVDTMPCLLSKLEFDEELRIELFGSFWKEYCGELNINDPNTWKEYVHLDDHLKLQDFLLSHLHNPLPFELEIRIKRKDGVYRWNLTRCTPTTNEKNRTSFLCATIDIDDQKKARATALELARLRSNFLANISHELRTPFSGFYGMLSLLDDTNLDSEQRDIVSAARISCEMLLRVINDLLNFSKLEAGKVTLESDLEFSLESVVCDCMQSVYSACAEKGINLSYNVSPDIPFFTAGDGMKIGQMLKSILDNSVKTVNNGFIRVRAFLAGSSKKNDRDQLQIAFIVEDTREESNAIFLANMINSLNRGCNDYLPMDLSGTALGMSTCLQLCKIMGGSVSVEVSQNNPTFKICYDLKIHELGKERYDIIATPLFQNLTEFNDLIKSKVAIRVSKTSTEYDNITTYLQAARKVLHVFKGLQDLASIFDLSPDSALLRCSVVVVDVYSMDDVKAVEKILKSYPDVHVIYLCCDPSRLNIEQELQKPSGRSFACKKRWGFLQMPCTRENFLKVTLQVFKSNEDTCNFYSYVNEYGESPKPDDDMDRLNKCVGSKILIAEDNPIVRMTLKKQLEHLGMDVDAAEDGKETLQIFESHPDNYYQVCFVDYHMPVYDGLEVTRRMRKIERKHGCAPLPIFALTADMQPTMETQFQEVGITHYLSKPFKKETLIKMLLQYLVNGTDGNANTS

Target ORF information:

RefSeq Version	NM_001023370.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe histidine kinase Mak3 (mak3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023370.2

ORF Insert Sequence:

ATGTATTCTC AGCATGAACT TCGTAATAAA GTCAGCCTAG CACTCTCGAG TCTACTTAGG 
TACACGTTTG AATTGACGCC TTTTTTTGAA CTGTACGAAG CTGATTTCGC ATATGCTTTG 
TATGCTGGCT TTGAACTGGC CACAAATCGA AAGGTGGTTG GAAAGTTCTC ATTTCAAAAT 
GTTCATCTTG AAAACGAGTA TAACATACTT ACGGAAATTG CAAAAGATGA AAGGGCATCG 
AAATTTAGCC CTACTCCTAT TGAGTTTACC TCTTTCCCCC ATATTGATTT ATCTGCTTGT 
ATTGCTTATG ACTTTGGCCA CGGAGCTGAA TTATCGACAA GTTATGCCTA TTTTAGAGAG 
AACCCAGCAG AATTTGTTCG CTTTTGTATT GCAATTTGTA AATGCATTGA ATACTTGCAC 
TCAAAAGGAA TGGTACATGG AGAAATACGT CTGGATAGTT TCATCCCCAT AAGCTCTTAC 
GACAATGTTT ACATGCTCAC TGTGGGATCA GGCGCTAGCT ATTTTCATAA TTGTTTACAA 
GCGCATAATT GGCGTAAATA TTCCGAAGAC TCAGAATCGA TGTCCAGAAT TTTGTTTATC 
AGTCCCGAGC AAACGGGCAG AACTTCATAC AGTGTCGGAT ATCGTACAGA TATATATAGT 
TTAGGGGTTT TATTTTTCCA TTACCTTTCA GATTGTTCTC CTTATACGGG ATCTTTTGTA 
CAACGAATTC GATCTATTTT GACAGAACCT TTACCCGACA TCAGTAAATC ATGTCCCAAA 
CTTCCGCATT TGATTTTTAA AATTATTGAA AAAATGACAC GAAAAAACCC AGATGAAAGA 
TACACTTCCT GTTCTGGTAT CGTTAACGAC TTGGAAGCTT GCTTGGATGA TATTGACAAA 
GGGTTAATAC TCAATGATCA TGTTTTGGAA AAAACAGGAC GTACATCTTT ATTCTATTTG 
CCTTGTTCTA TATATGGTCG TGAACATGAA ATCAAATTAA TCAGAAAAAT CTTAAGAAAT 
TCCCCGCGCG CAATAAATCA CCAAGACAAA AAGGATTTGG AGACATTTAA TCCATATTAT 
TTAAATGCGA TAGAATCTGA GAGCTCTTCT CAATCCCTCT CTTTATCCCA AAGGGCTTCT 
GAAGTTATGC CACTGGTAAT ACTTATCACA GGATGTGAGG GTATTGGAGA ATCAAGCTTG 
ATTCAAACTA TTTGTGATCG ACGTGAAGGG TATATGGCTA TCACAAAATT TGAAGTATCA 
CAATCAATTG TATACTCTGC GATTGTTTCA GCTGTTGCCG AGTTTATTCG GCAAATCCTT 
GCTGAAGATC AGCTTTTACT TAATAATTTT TTTGAAGAGC TTAAGAATAA ATTAGAATCG 
GATTTGTATT TGCTCGATTC GGTTTTCGAT TTGGTACCAG AAATTAGAAG TTTATTACAA 
CAGTTTTCGA CTTCTTCTGG TAATACTAGA AAAACGTCTT TGTTGGGCTC GAATCATTCT 
AGCTATTCCG ATAAACTTGG GTCTCCTACA ATTCTCTCAA CTTCGTTTTC ACTTGCAAGG 
CCATATCCTG AGCCGGCTCT TGTAAGTCCT TCGACTGAAA GGCCCCCTAG GTCAAGTTTT 
TCTGCCGCCT TGATGACCCT GCTAAATATC ATTGCTAGTT TTAAAAAAGT AACGATGGTT 
ATAGAGAATA TTCATCTTGC TGATGAGTCT TCTTTAATTA TTCTCCAGAA AATCGTTTAC 
TCTGATCTTC CACTTACCTT GATGATTACT TGCGATAAAG AAAACGATCA TGTAATTAAC 
AGGTTTCGTT TAGCGAATGA CAGGATACAC GAAATCGAGT TAAAACCACT GTCTTTTAAT 
GCTGTGAATT CCTATGTTCA GGCTACTTTG CATCGAACCG ATGATGGATT AGCAAGATTT 
TCTTCTTACG TTTATCACAT TAGCAAAGGT GTGCCTTTAC TTGTGAGAAA TGTACTACTG 
AGTATTTATG AAAACAAGAT AATTTACTTT GATTGGAAAA AAAATCGATG GGAAGTAAAT 
TACGACGAAA TGTACACTCT TGACAATGAT TATTCTGAGC CCGATGCATT TATGACGGCC 
AAGAAAAAAA TCAGTAAACT GAACGACTCT TCTCGTGCTA TCCTTGGTTG GGCTAGTCTT 
TTGGGCCCAT CTTTTTCTTT TGCAACTGTA AAGAAGCTTT GTAAGGATAC CGATAATATT 
GAATTAAATG TGGAGGCTCT TCAGTCCGCA TTAAGAGAAG GTATAATTTA CGCCACTTCT 
TCTGATGACA CGTATACGTT TTCAAGGTCT ATTTATGTTA AAGCGATGCG TGATTTGCTT 
AATGAAGCAA AAATACAGAT TATGCATGCA TGTCTTATTG ACGTTTGTCT TAAAAATCGA 
GATCGTTATA ACATCTTCGA TATCGCTTTT CATATCAATG CCGCTTTTGA TTTTGTTAAG 
GGTGATAAAC GATCTGTTGA ATATTGCCAT TATTTGCACT TGGCTGCCGA AGAGGCTTTA 
AAGATTGGAG CTAATCAAGA GGCGCTTGAC TTATATAACA GATGTATAAA AATGATACCA 
CACGAAATTC CTGAGGAAAG TGATGATAGT TATATTCGCT GCCAGCTTAT TGGTATGTAT 
GTTGGATGCG CTGAAGCTTA TTGGGTAAAT GATAATTTCG ATACAGCTTC AGAAATGTTA 
AAACTAGCAG AGGAGAAAGC TTGTAATAAC TCGGAGGTTT TTCCTGCAAG GTTTTTGTAC 
TCTCGGATTT TATTCGAAGG GGTGCATATA GAAGAGTGCA CTCAATATGT ATTATCTTGT 
TTGAAGCCAC TTGGGTATGA GTTAAAGCGA CATTCTCTCG AAGATTCAAA GTCAATTATT 
TCAGCACTGA TCCCACGTAT TATTGACAAA ATTACTAAAA GCTCAGAGGA ATCTCAGTCA 
TCAACAGATG ACGATGACCG GAGAATTTTC GAAATACTTT CTTTTTTATA CGTCGGTTCA 
GTCGCTACTT CTTACTTTTC AGAGACCGCA GAAATGGCCA TTGATTTTGG AATAGCACAA 
GTCGAATTTT TTTTAAGTAC TGTTGTCAAT TCGTTCTCCG CTTTTGCCTT AGTTTATTTT 
GCTATTCTTG CAAATTCTTT ACTTGAGCCT TCAGAAGATA TTCTCTTCAT CGGTAATTAT 
GGAGAGAAAC TGAATCGTGA AGCTGAGAAT CCTATAATAT TTTCACGTAC TGAATATTTG 
TATGTTCAAT CTCTTGGTTT TATAGACAGT ACCACGAAAG AGAGAAGACT TACTATTGAT 
TATTTGGACA GAAATTGTGT CACTTGCAGT GATAAACACG TTATTATTAG TCTGCTTTTA 
GTGTCATCAT GGGAGAAATT TCTAACTTCC AACAACTATT CAAATTACTT GGCAGATTTT 
GAAACTACTC ATGCGCAAAT TATGGAAATG AAGCCTTGGG TTGGTGATAC CTCATTAATA 
ACACAATTAA AGCGATTTTT GATGTGCTTA CAGGATAACA TCAAATTGGA TTTAATCAAG 
TCAAAGAGTT TTTTGTCGGA TCATAATATC CAATTATCAT CCCCAGCAGC ACAAGAATCT 
GCGAAACTTG CATTCAGCCT TCACGGATGG ATTAACTCAT GGTATCTTCT GGCTCTTGTG 
ATGCATGGTG AATGGGATAT GGCTATCAGT TATGGAGAGA ATTTTAAACG TGAATTTAAA 
AATGCGCTTT TAACTTCTTC TAGGGTATTT GGAATTTTTA TGTTTACTTG GTCTTTGGTC 
AACAAGATGC TCATTTGTCC CGAATTCACT AAGCAAAAAA AATATTATGA GCAGTATAAA 
GAAAATCTTG GATTTTTTGA TAGCCTATGC ATTGGTGATA ACGAATGTAT CACTCGTGTA 
TATTTTCTTT TATTAAAAGC ATGTGGTTTA ATAATGAATG GGCTGAATTT TGAAGCATCA 
GTTATGCTGG AGGAAGTCAT CTCTTTAACA GAAAAACTTG AACTTTTTTT GTTACAGGCA 
TTTGCATTTG AAACTGTTGG AAGCATTTTT GTGTCTATGG AACTATATAC TTCTGCTACT 
CAATACTTGG AAGAGGCTAT TCGAAATTAT GCTGCTCTGG GTGTTAAACA AAAAGCTAGG 
CATTTGAGGG ATAAGTTCGG TGATTTGTTG GTTTCGAACA ACTTACAGGT TTCGATTGAT 
GAAGCTACAC AAACAGATTT CCCTTTGGTG TTTAGTCCTG AGCGCTCAAG TATTGACATA 
AATGCTAGTA GTATGCGTTC TGAAAAAGCG TCCTTTGAGA TTCCTTTTCC TGAAGAGCAG 
ATTGATGATG ATGTTTCTCC AGTAGCCCAA GATTCTTCTC TGGAAGAGTT ACTTATATCT 
TTGGACATCA TCGATCTAAC CTCAGTAATG AGATCCTGCC AAACGATTGC CAGTGAAATT 
GAGTTGACTG GTTTGCTCTC GACTATGACA CAGAGAATGT TGGAAGATTC TTCAGCTAAC 
GCTGCTGTTA TAGCAATTCG TGATGACGTT GGCTTTAAAA TTGCAGCTTA TCGTACGGGA 
GAGCTTAACG AAGTTTTTGC TCCCCCGATG CCTATTACAG AAGATCAAAC GTACGTTCCT 
TCTAGAGTGA TAAATTATGT TGTCCATACG CAAAAAGCTT TGTTTTCGAA TAATATAAAC 
CATGAATTTG ATTTGCAGCA GGAGCGTTGG AATATCGAAA ATCATATGGG GAGAAGCGTA 
ATTGCTATTC CTTTATACCA AAAGAAGGAG GTTTTTGCGA TACTCTACTT GCAAGGCCCT 
CCATCAGCAT TTCATTCTCG ACATATGTCG GTACTATCAA TCCTTGGGGC TCAGGCAAGT 
TTCGCAATTG TGAATATATC TTTGTTTCAT AAGGTGAAAG AGGCAACTAA TGTTAATACG 
ATTATCATTA AAGCCCAGAG AGAAGCATTA AATTTGGTGC AAAAATCGGA GGCTAAATAT 
CGCAGCTTTG TCGATACAAT GCCTTGCTTG TTATCAAAAT TAGAATTTGA TGAAGAGTTA 
AGGATTGAGC TTTTTGGAAG TTTTTGGAAA GAATATTGTG GTGAATTAAA TATAAACGAC 
CCAAATACAT GGAAGGAATA TGTTCATCTT GACGATCACC TTAAATTACA GGATTTCCTG 
CTCTCTCACT TGCACAATCC TCTTCCTTTT GAACTAGAAA TAAGAATTAA AAGGAAGGAT 
GGAGTTTATC GATGGAATCT TACACGCTGT ACCCCTACGA CGAACGAAAA AAATAGAACT 
AGTTTTTTGT GTGCAACAAT TGATATTGAC GATCAAAAGA AGGCACGAGC TACCGCATTA 
GAACTGGCAC GTTTGCGTTC GAATTTCTTG GCGAACATTT CACACGAATT AAGAACACCT 
TTTTCTGGCT TCTACGGCAT GCTTTCTCTC TTAGATGATA CAAATTTAGA TTCTGAGCAA 
AGGGATATTG TTAGTGCTGC TCGTATAAGC TGTGAAATGC TTCTTCGGGT AATCAACGAT 
TTGTTGAATT TTAGCAAACT TGAAGCGGGC AAAGTCACTT TAGAATCTGA CCTTGAATTT 
TCTTTAGAAT CTGTCGTTTG TGATTGTATG CAATCTGTAT ATTCAGCTTG TGCCGAGAAA 
GGTATCAATT TATCTTATAA TGTTTCTCCA GATATTCCTT TTTTCACAGC GGGAGACGGC 
ATGAAAATTG GACAAATGTT AAAGAGTATC CTTGATAATT CGGTAAAAAC AGTTAACAAT 
GGATTTATCC GTGTTAGGGC CTTTTTGGCT GGTTCATCGA AAAAGAATGA TAGGGACCAG 
TTACAAATTG CGTTTATTGT AGAGGATACT CGCGAAGAAA GCAATGCTAT TTTTTTGGCT 
AATATGATCA ATTCCTTGAA TCGTGGCTGT AACGACTATT TACCCATGGA TTTAAGTGGT 
ACCGCACTTG GAATGTCCAC GTGTTTACAA CTTTGCAAAA TAATGGGTGG ATCAGTAAGT 
GTAGAGGTAT CACAAAATAA CCCTACATTT AAAATTTGTT ATGATCTGAA AATTCATGAA 
CTTGGAAAGG AAAGATACGA CATTATAGCT ACTCCTCTAT TTCAAAACCT AACAGAGTTC 
AATGATCTCA TAAAATCAAA AGTTGCTATC CGAGTTTCTA AAACTTCTAC TGAGTATGAC 
AACATTACTA CATATCTTCA AGCTGCGAGA AAGGTTTTGC ATGTTTTTAA GGGATTACAA 
GACCTAGCAT CAATTTTTGA CTTAAGCCCT GACTCTGCAC TTCTCCGCTG TTCCGTTGTG 
GTAGTGGATG TTTATTCGAT GGATGATGTT AAGGCAGTCG AAAAAATATT GAAAAGCTAT 
CCGGATGTAC ATGTCATATA TTTGTGCTGT GATCCCTCTA GATTGAACAT CGAGCAGGAA 
CTACAGAAAC CTTCAGGAAG ATCGTTTGCA TGTAAAAAAA GATGGGGATT TCTTCAAATG 
CCTTGTACTA GAGAAAACTT CCTCAAGGTT ACATTACAAG TGTTTAAGTC TAATGAAGAT 
ACTTGTAACT TTTACTCTTA TGTTAATGAG TACGGTGAAT CCCCAAAACC AGATGACGAT 
ATGGACCGGT TAAACAAATG TGTTGGATCA AAGATTTTAA TTGCTGAAGA CAACCCCATA 
GTGCGTATGA CTTTAAAAAA GCAACTAGAG CATTTAGGAA TGGATGTTGA TGCCGCAGAA 
GATGGAAAGG AAACTCTTCA AATTTTTGAG AGTCACCCCG ACAACTATTA CCAAGTTTGT 
TTTGTTGATT ATCATATGCC TGTATATGAT GGCTTAGAGG TAACCAGAAG GATGAGAAAG 
ATAGAGCGTA AGCATGGTTG TGCACCTCTT CCCATCTTTG CTTTGACCGC CGATATGCAG 
CCTACCATGG AAACTCAGTT TCAAGAAGTT GGAATAACGC ATTATCTCAG TAAACCTTTC 
AAGAAAGAAA CACTAATTAA AATGCTTCTG CAATATTTAG TTAACGGAAC TGATGGAAAT 
GCTAATACTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mak3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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