ORF » Species Summary » Schizosaccharomyces pombe » brl1 cDNA ORF clone

brl1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following brl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99607 NM_001023487.2
Latest version!		 Schizosaccharomyces pombe ubiquitin-protein ligase E3 Brl1 (brl1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    brl1 ( NM_001023487.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588497.1 Schizosaccharomyces pombe ubiquitin-protein ligase E3 Brl1 (brl1), mRNA.
    Price & Availability↑
    CloneID OSc99607
    Clone ID Related Accession (Same CDS sequence) NM_001023487.2
    Accession Version NM_001023487.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product ubiquitin-protein ligase E3 Brl1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023487.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGCGTTG TGAATGAATC GAGGAAAAGA AGACGTCTTA TTCACAGCGA AAAAGAAACG 
    CGTCGTTTAG ACCTCGGAGA AGAAGATGAC TTAGAGTTAT TTCAAAAGGA AGCAGCTTGG 
    CGAAGATCTA GAGAGTATGA GCATCTCACT GTTTTGGAAA AGAAATTATA CGAGTATTGG 
    GAATCTCTAG TTTTGAAACT TGATCATTAC GTTGCTGCTG CTCTGGAAAA TCATGAACAA 
    TCATTTGAAG AACTAGAGAA AATCACTGCT TCTTTATATC AACCTGATGA TAATTTACAG 
    ACACTAGATT TGTCACTTGC TGAGTTCAGT TTAATCAAAG ATGCGCAAAA CTATTTGAAT 
    AAGTATGCTT CCTATTTTCA AGCCCATGAA CCTACTCTAC AAAAGTTAGC TAAATTTGGT 
    TCTTTTAAAT TAAAAGAAAC TCCAAACGTC CATGAATGGA TAGACCAACG CCAGGTTCAT 
    TTTACAAGTA TGATTAATTA CCGTACTAGC CAACTTAAGT TGGCACTCCT AAAAAGGAAA 
    CTTTCCTTCT TTCGTGAAGC TTTGAATTCA GCTGAATTAG AATGGAAACG GGTGCAGCAA 
    GATCAGCTTA CTAGTGCTTC CGAAAGAAGT ATTGAAAATA TCGCTGATGA TATCCCTGCT 
    TCGGAACCAA AAGCAATTCT CGAAAATGGG GAAGGATGTT TAAATGATAA TGATAATATT 
    TCAAAACTAA AAAACAATTT TCAATCTCAA GCTGATTTGA TGCAAGCAAA TATAAATTCC 
    AAATTGGATG AACTTTCTCA AAAATCAACT CGTGCAGCAC AATTGTACGT CGACATTATG 
    GGTATAACGG ATGAAAGAGT GAGTAGAGAG CCCCATTATT TAGAGCTGAA GGGGGTTCTT 
    TCTTCAAATG AAGAGAAAAT CGAATCCATT AATCGGGACC TCTCTTCATT GTTTGAGGAC 
    ATTCAGTTTT TTATTTCTCA ACGGACGAAA CGACAGAAGG ATATTATTGA TTTAAAATTG 
    GCATGTGTAA AAGAGAAACA ACAACTTATT AAAACCCTTG AATCATCTTT AACCCAAATT 
    CGTAACGAAC GAGACTCTCT AGTGGCGAAA CAACAAATGC AATATACTAA CAATCTATTT 
    TTTGACGATA TGATGCTTTT ATTAAGCAAC TTGTCAAACG CTCGGGTTGC AATTCTAGAA 
    GGTTATTCAA ATCGATTGTG CATATGGGAT CGTATTGAAA GGTCTAAGAC TGGTGAGATG 
    AATAATGTCC TAGATGAAAA AGAAGAAATT TCTGCGTCTT CAGCTTTGGA AAAACTAATA 
    AAAAATAATA GTTGTTTGGA GGCTGAACTC CCAAGTATGT ATGCAGCATT TGATCAATCA 
    CAATCTCGTT TGTTGAAGAA ATATGAAGAA CTTGAAACGA AGGAGAAAAA AGCTTTGGAA 
    ATGCATTATG AAAAGGCTCG TGCCACCCAA AAATATTTTG CTGCCATGAA AGCTCGGGAC 
    ATTCTTATGA CAGAGAAGAA AACTCTTAAA CTGGCCGAAA ATAAAGAACA TGATTATATA 
    GGGAAGTTGC AAGAGCGAGA ACATGCGCTA ACAAAATATG AATCCTCTTT AAAAGCTGAA 
    TTGGAAGTTT ATAAACAAAT AAAGGAAATC TACGGGAAAC ACTCGGTCGA AGTTTTAACA 
    GAAGATAAGC ACTTACAAGT TAAGCAAACA AAACTTACTC AAAAACTTGA AGATCTTATA 
    GAGTCTGTTC AAAAATCTGG GGAAAAGCTT ATGATCATGC ATCAGAAGTT GTTTCATTTG 
    CAAGAAGAGC ATACTATTTT ATCCATAAAA GCTTCGTATA ATAAGAAAGA GTCTCATCTT 
    ATAAACCAGG CATATGAAAC ACAAGAGGCT CAAGTATACA AAGGAATGTT AAAATGCTCC 
    GTGTGCAACT TTTCTAATTG GAAATCTAAA CTCATTCCAA ACTGTGGTCA TGCCTTTTGT 
    TCTAATTGTA TGGAACCTTT TTATGAGCAT AAAACAAGTA CATGTCCGCA GTGTGAAACA 
    CCTTTTTCGG TTTCCGACAT TTTGACCATT CATCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588497.1
    CDS157..2235
    Translation

    Target ORF information:

    RefSeq Version NM_001023487.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe ubiquitin-protein ligase E3 Brl1 (brl1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001023487.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGCGTTG TGAATGAATC GAGGAAAAGA AGACGTCTTA TTCACAGCGA AAAAGAAACG 
    CGTCGTTTAG ACCTCGGAGA AGAAGATGAC TTAGAGTTAT TTCAAAAGGA AGCAGCTTGG 
    CGAAGATCTA GAGAGTATGA GCATCTCACT GTTTTGGAAA AGAAATTATA CGAGTATTGG 
    GAATCTCTAG TTTTGAAACT TGATCATTAC GTTGCTGCTG CTCTGGAAAA TCATGAACAA 
    TCATTTGAAG AACTAGAGAA AATCACTGCT TCTTTATATC AACCTGATGA TAATTTACAG 
    ACACTAGATT TGTCACTTGC TGAGTTCAGT TTAATCAAAG ATGCGCAAAA CTATTTGAAT 
    AAGTATGCTT CCTATTTTCA AGCCCATGAA CCTACTCTAC AAAAGTTAGC TAAATTTGGT 
    TCTTTTAAAT TAAAAGAAAC TCCAAACGTC CATGAATGGA TAGACCAACG CCAGGTTCAT 
    TTTACAAGTA TGATTAATTA CCGTACTAGC CAACTTAAGT TGGCACTCCT AAAAAGGAAA 
    CTTTCCTTCT TTCGTGAAGC TTTGAATTCA GCTGAATTAG AATGGAAACG GGTGCAGCAA 
    GATCAGCTTA CTAGTGCTTC CGAAAGAAGT ATTGAAAATA TCGCTGATGA TATCCCTGCT 
    TCGGAACCAA AAGCAATTCT CGAAAATGGG GAAGGATGTT TAAATGATAA TGATAATATT 
    TCAAAACTAA AAAACAATTT TCAATCTCAA GCTGATTTGA TGCAAGCAAA TATAAATTCC 
    AAATTGGATG AACTTTCTCA AAAATCAACT CGTGCAGCAC AATTGTACGT CGACATTATG 
    GGTATAACGG ATGAAAGAGT GAGTAGAGAG CCCCATTATT TAGAGCTGAA GGGGGTTCTT 
    TCTTCAAATG AAGAGAAAAT CGAATCCATT AATCGGGACC TCTCTTCATT GTTTGAGGAC 
    ATTCAGTTTT TTATTTCTCA ACGGACGAAA CGACAGAAGG ATATTATTGA TTTAAAATTG 
    GCATGTGTAA AAGAGAAACA ACAACTTATT AAAACCCTTG AATCATCTTT AACCCAAATT 
    CGTAACGAAC GAGACTCTCT AGTGGCGAAA CAACAAATGC AATATACTAA CAATCTATTT 
    TTTGACGATA TGATGCTTTT ATTAAGCAAC TTGTCAAACG CTCGGGTTGC AATTCTAGAA 
    GGTTATTCAA ATCGATTGTG CATATGGGAT CGTATTGAAA GGTCTAAGAC TGGTGAGATG 
    AATAATGTCC TAGATGAAAA AGAAGAAATT TCTGCGTCTT CAGCTTTGGA AAAACTAATA 
    AAAAATAATA GTTGTTTGGA GGCTGAACTC CCAAGTATGT ATGCAGCATT TGATCAATCA 
    CAATCTCGTT TGTTGAAGAA ATATGAAGAA CTTGAAACGA AGGAGAAAAA AGCTTTGGAA 
    ATGCATTATG AAAAGGCTCG TGCCACCCAA AAATATTTTG CTGCCATGAA AGCTCGGGAC 
    ATTCTTATGA CAGAGAAGAA AACTCTTAAA CTGGCCGAAA ATAAAGAACA TGATTATATA 
    GGGAAGTTGC AAGAGCGAGA ACATGCGCTA ACAAAATATG AATCCTCTTT AAAAGCTGAA 
    TTGGAAGTTT ATAAACAAAT AAAGGAAATC TACGGGAAAC ACTCGGTCGA AGTTTTAACA 
    GAAGATAAGC ACTTACAAGT TAAGCAAACA AAACTTACTC AAAAACTTGA AGATCTTATA 
    GAGTCTGTTC AAAAATCTGG GGAAAAGCTT ATGATCATGC ATCAGAAGTT GTTTCATTTG 
    CAAGAAGAGC ATACTATTTT ATCCATAAAA GCTTCGTATA ATAAGAAAGA GTCTCATCTT 
    ATAAACCAGG CATATGAAAC ACAAGAGGCT CAAGTATACA AAGGAATGTT AAAATGCTCC 
    GTGTGCAACT TTTCTAATTG GAAATCTAAA CTCATTCCAA ACTGTGGTCA TGCCTTTTGT 
    TCTAATTGTA TGGAACCTTT TTATGAGCAT AAAACAAGTA CATGTCCGCA GTGTGAAACA 
    CCTTTTTCGG TTTCCGACAT TTTGACCATT CATCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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