nup211 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup211
Entrez Gene ID	2538843
Full Name	nucleoporin nup211
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023226.2	NP_588236.1	nucleoporin nup211

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_00594	XP_003718445.1
Schizosaccharomyces pombe (fission yeast)	nup211	NP_588236.1
Neurospora crassa	NCU04059	XP_957674.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors showed that Nup211, fission yeast homolog of Mlpl/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery.
Title: Nup211, the fission yeast homolog of Mlp1/Tpr, is involved in mRNA export.

The following nup211 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup211 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99288	NM_001023226.2 Latest version!	Schizosaccharomyces pombe nucleoporin nup211 (nup211), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup211 ( NM_001023226.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588236.1 Schizosaccharomyces pombe nucleoporin nup211 (nup211), mRNA.

Price & Availability↑

CloneID	OSc99288
Clone ID Related Accession (Same CDS sequence)	NM_001023226.2
Accession Version	NM_001023226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	nucleoporin nup211
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023226.1.

ATGCACGATT CTTCCTGGAC AGAAGCTGAT ATACTCGGGG TATGCAGTTT TCTAGACATA 
CCAAAGACAA AAATTGATCC TTTACTTCAG GTGGACGCGT TTACATCCAT ATTAATACCT 
TTAATTAGTA AAAGCAAAGA TTATGAAAGT ATAAAAAATG ATCGTATAGT TACAGAAGTC 
AATTACGAGC AACAATTGCG AAATTCGGAG AAGAAACTAC TTCAATCAAA CGAACGTTAC 
GATTTATTAG AGGATGAGCG CAAGCTTTTA GAGAATGAAC TCTCTCAAAT CAAAGAATAT 
CTTAGGGAGA AATCCTCTTC ATATGACACT GTTTTGCACG ATTGCAGTTC TCTCAAAAGT 
GTAAATGAGG CATTAAAACA AGCTCAGGAT CAAAATTTGA AGCAAACTGC TCAACTTCAA 
AATCTTTTAT CAGATAAAGA AAAGGAAGTT GAAAAGAAAA TCACTATCAT TAAAGATCTA 
AAGGATGCCC TTGCCAGTTC TACCCATCAA GTTTTGGAGC TTCAACATAC ACAACAAGAA 
AAAGCATCTT TGCAGACTAA CTATGAGTTT GAGTTGCAAA AATTAACGCA GAAAAATTCG 
ATTTTGGAAA ATAATAACAC CTGGTTAAGC CGAGAGCTTC AGGGTGTGAA CGACAAATTG 
TTATCTCTTC ATCAGGAGGC CTCTTTGGAA AAGTCTCAAC TAAGTTCTCA ATTATCTGAC 
GCTGTTTTGG AAAAAGATGC GCTACAACGG AAAGTGAGTT CTTTGTCTCA ACAGTTTACA 
GAAAGTAATT TACGATATCA GAATATTGTA GCTGAACTCT CCGAAATGCG AAAGCAATAT 
GAGTTTTCGC AAGTTTCTTT TGAAAAAGAG ATTTCTTCAC AAAAGCAAAT TAGTGAGTTG 
TGGATGGAGA AATGTGAGGA TTGCTCTTTA CGGCTCAAAG AGTTGCAGAA CTCAAACGGC 
GAGTTGGAAA AGCTATTGGA AGCTGCACAA TCATCTTTTG AAGAACAGCT TGAATCTCAT 
AAGGAAGCAG AAGCTTCCTT GAAATCTCAA ATCAATTTTC TAGAGAAAGA GGTATCAAGT 
TTAGAGTCTC AGCTAAAGTT GGCTAATGAG CGTCTTCGGC ATTATGATGA AATAGAAATC 
TCAGATATGA GTGAATTGAA ATACTCAAAT TTACTAAATA ATAGTATGAA AGGCTTTAAA 
GGTCAGTCCT CCGTCTCTGA CTTGTATTCC GAGCGTTTAT ACTATAAACA GAAGTATGAG 
CAAACATGTC AAGAGGTTGA AAGGTTACAA CGTAGCTATA ACCATGTTAT GGAAGAAGCG 
AATCTTCAGC ATCCTTTGGT TAAAGAACAG TTTAAGCGAT TTGCTCATAT GCAACGGGAA 
ATTGTTGCAA TGAGTGAGCA ATATCAGAAG TCATTGGAAG ACTGTCAGAA AGCCAAGTCC 
CGTTACGAGC AATTAGAGAC ACTATTTAAA GATAAATGCA CAGAGAATAA GCATTATGAG 
CAAGAGACCA AAGATTTAGC GCGACAGGTG CAAGTTTTAC TTCATGAACT CGATTTGTGT 
GAGAACGGCA TAGTGCTAGG CGTGGATAGT AGAAAGAAGA TTAACTCCTA TGTTGAAAAA 
TCGCTCACTG AAGATGAAAC TGATACGGAT CAAATAATTT CAAGCAGGCT AGTTGTATTT 
CGTAATATTA GAGAATTGCA ACAACAAAAT CAAAATCTAC TGTCTGCAGT TCATGAATTA 
GCTGATCGTA TGGAGAAAGA CGAAAAACCT GATCTTGATG GAGCCGAGAT TCAAGAAGAA 
ACACTGATAA AAGCTAACGA AACTATTGAT CAACTTACTA AAATGTTGGA GGAGGTGAGC 
GATCAGTTAC GCTACTCTTT AAAGGAACGA GACTTTTTTA GATCACTAGT TCAAGAGAAT 
GAAAAACTTT TAGATATGGC GCCTGCAACT CCTAATTCCA AATTGAATAC TAATTTAATT 
GAGCAGACTT CTTACCAGCG TTCGCTTATT CGACTTGAAC AGCTAACTAA TGAACTGGAG 
TCCCTTAAGT CAATTTCTCG TAACAAAGAA AAAAAGTTTG AGGAAGCTAT CTCATCTCTC 
CAGCTCGAAA AATCTAATAT TCAATTGCAA CTTACCTCTT TAACATCTGA ACGAAGCTTA 
GCCCTTGAAA AGCTAAATGA TCTGGAAAAA TCACTTGTTT TATCTGAAAG AAGTAAAGAT 
GAACTTGATG AAAGCTATAA GTCTTTACAA GAGCAACTTG CTTCTAAGAA AATTGAAGTT 
CAAAATGTTT CTTCTCAATT ATCAATCTGT AATAGCCAGC TTGAACAATC CAATCATATT 
GTTGATAATT TAAAAAGCGA AAACCTGCTT TTAACTTCCG TCAAGGATAA GTTGAAAGCC 
GATTTATCTA ATTTGGAGTC GAAACTTTCG TCCCTGCAGC AGGATAATTT CCATATGAAA 
GCTCAAATTG AATCTTCAAA TCAAGAGTAT ACGGCAACAG TGGACTCAAT GAATAGTCGA 
ATACTTGAAC TTTCTAATGA TCTTCGAGTT GCTAATTCAA AGCTATCTGA GTGTTCCGAT 
GATGTTCGTA GACTTACTCT ACAAAATTCA TTTGATTTGA GGGAGCATCA AACATTGGTG 
CTTCAACTTC AGTCTAACAT CACTGAGTTA AAACAAGACA TAACATTGCA ACGCACCGTG 
CGAAATCAGT TAGAAATACA AACCACTGAG TTGAAAGAGC GCTTAAAGTT TATGGAAGAA 
CGGCAGGAGA ACTTGCAATC TAAATTAATC GCGGCTAATA AAGACACGAC ACAAAATCCT 
GATAATGTTG AAGTCGAGGC TATATCTATT GAACTGGAAA GGACGAAGGA AAAACTTCGC 
ATGGCCGAGC TTGAAAAGAG TAATATTCAA CAAAAATACC TCGCTTCAGA AAAAACCTTG 
GAAATGATGA ATGAAACCCA TGAGCAGTTT AAGCATCTAG TAGAATCTGA AATTAGCACA 
AGGGAAGAGA AAATTACGTC CCTTCGCTCT GAGTTGTTGG ATCTTAATAA GAGAGTTGAA 
GTACTAAAAG AAGAGAAAGA GTCTTCGTCC AAGGAACTTG CCAAACAACT TGAGGATGCT 
GTTCGTGAAA AGGATAGTGC ACTTTCTTTC AAAAAAGATT ATGAAAAAAT TCGTTCAGAT 
GCGGATCGTG TTATTACCTC TTTGAAAGAA GATATTGAAA AAGAAAGATC TCTAATGAAG 
GAGTGTCATT CTAATTACGA ATCAGAGATC GTTAGTCATG GGCGTACCAC TCAAAAATTG 
CGGGATTTAC GTACTGAATT TGACGAGGTG AATACGAAAT ATTTAAAGCT CAAAGCAAAT 
TTTGAACAGC AGCACTCAGG ACTTTCAGGT GCTGAAAAGG ATTGGAATAT TCAGCGGAAA 
GCCATGGAAG ATGAAATTAG CTCCTTGAAA GATTACATTT TGGGACTTGA AAATCAAAAT 
AAACTACTAC ATTCGCAATT TGATTCTCTT AGTCAACAAA TAACTGTTCT TCAACAGAAT 
TCTTCTGAAA ATTTAAATAT ATCTGCCAAT TTAGAAGCCG TTCAAGATAA CGATTTACGT 
GAACTTGTTA GCTACTTGAG GCACGAAAAG GAAATAATGG ATAATAAATA TGAGTTAACA 
ATTCTGGATA ATAGAGGTTT GAATCAACAA GTCAAGTCTT TGCAATCCAC CGTTGATTCA 
TTGCAACTCG AGCTCAACCG TCTGCAGTCT CTGCCTGTGT CAAATGACCA AACAGATACC 
CCCATTATCA GCGGAAGTCA AGAAGTTCAG TTACTTTATG AAAGTAATAG TGTCCTTAGA 
AAGGACAATG ATGCTAAACT TGGAAAAATA CAAGAGCTGG AGAAAGAGGT TGAAAAACTG 
AATGCTTCAT TGAATCCCTT ACAAACTGAA ATAAATGAAC TTAAGGCAGA AATAGGCGCA 
AAGACAGCAA GTCTGAACTT AATGAAGGAA TACAATTCTC GATGGAAATT AAGATTTCAG 
TCCGTTTTGA ACAAGTATGA GCGTGTTGAT CCCACACAGT TAGAGGAGTT AAAGAAAAAT 
TGTGAAGCAT TAGAAAAAGA AAAACAAGAG TTGGAAACGA AACTTCAGGA GACAGCAAAA 
GAAACCGATA CATTTAAGCA ACAGGTTAAT TCTCTAAACG AAGAGGTGGA AAATTTGAAG 
AAGGAAGTTG AGCAGGCAAA CACCAAAAAT ACTAGATTGG CAGCTGCTTG GAATGAAAAA 
TGTGAAAATT TGAAAAAATC CAGCTTAACA AGATTTGCCC ATCTAAAGCA GGAATTGACC 
AACAAAAACA AGGAGTTAAC CTCTAAGAAT GCTGAAAATG AAGCAATGCA AAAAGAAATA 
GAGTCTCTAA AGGATTCCAA TCATCAATTA CAAGAATCGG CTTCCAGTGA TGCTGAACAG 
ATCACGAAGG AACAATTTGA ACAACTCAAG TCGGAGAAGG AAAGAACTGA AAAAGAGCTA 
GCAGATTCTA AGAACGAGCT AGAACACTTG CAATCTGAGG CTGTCGATGC TGACGGTAAA 
ACTGAAATTT CAAATCTTGA AAAAGAAATT CATGAATTAC GGTCTGACAA AGAAGGACTT 
GTGCAACAAG TACAAAACTT GAGTGCGGAA TTGGCAGCTT TACGAGAGCA TTCACCGACC 
CAAGGAAGTT TAGAGAATGC TGATGAGATT GCAAGGCTAC GTTCACAACT TGAATCTACG 
AAACAATATT ATGAAAAAGA AAAGGAAACC GAAATTTTGG CTGCTCGCTC TGAACTCGTC 
GCAGAGAAAG AAAAGACAAA GGAAGAGTTA GAAAATCAAC TCAACGAAAA GTCACAGAGA 
ATAAAGGAAC TTGAGGAACA AGCTCAAAAA AATTCTTCGG AAAATACTCA TGACAATATT 
GATGACATGA TTAAGCAGCA GGTTGAAGAA AAGCTTAAAG AAAATTCTGC CAACTTTGAT 
GTTAAGTTGA AGAAGGTGGT AGCTGAGACA GAATTTCGGA GCAAAGCTAA AATATCTGTG 
TATGAGAAGA AAACTAGGGA TCTTCAGAAC AAAATTACAC AACTTGAAGA AACTATTGAG 
AATTTGAATA AACAGCTATC CAATCCTGAA AAGACTGATG AATCTACGTC TTCTGTAACA 
GAAACAAAGC CTGTTACTTC CAAACCAACA GCTAGCAAAG CTGATGTTGG TCAGAATGCT 
ACAGAAGCTT CGTCAGCCAA GAGAGAACCT TCCGGTAAAT CTCTGAGTGC AAGACTTCAA 
GGAACAGGCA AACAAAAAGG TGTTCAACGA CCCGCTGTTT CGCGTCCTGT TCCTATGAAA 
CCAGACAGTG GAAAACTCTC AATAACTGGA GCTTCCAAAC GAATTGCTAC TTCAAAGAAT 
GCTGCCCAAA ACGCTAAAGA ACTGTCAAGC ACTGCTAAAT CCGGCTCCCT TAAAAGACAA 
CGTGACGATG CGAACAAAGG AGGATCCAGT TCGAACCAAA AGAAAGCAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588236.1
CDS	123..5636
Translation	MHDSSWTEADILGVCSFLDIPKTKIDPLLQVDAFTSILIPLISKSKDYESIKNDRIVTEVNYEQQLRNSEKKLLQSNERYDLLEDERKLLENELSQIKEYLREKSSSYDTVLHDCSSLKSVNEALKQAQDQNLKQTAQLQNLLSDKEKEVEKKITIIKDLKDALASSTHQVLELQHTQQEKASLQTNYEFELQKLTQKNSILENNNTWLSRELQGVNDKLLSLHQEASLEKSQLSSQLSDAVLEKDALQRKVSSLSQQFTESNLRYQNIVAELSEMRKQYEFSQVSFEKEISSQKQISELWMEKCEDCSLRLKELQNSNGELEKLLEAAQSSFEEQLESHKEAEASLKSQINFLEKEVSSLESQLKLANERLRHYDEIEISDMSELKYSNLLNNSMKGFKGQSSVSDLYSERLYYKQKYEQTCQEVERLQRSYNHVMEEANLQHPLVKEQFKRFAHMQREIVAMSEQYQKSLEDCQKAKSRYEQLETLFKDKCTENKHYEQETKDLARQVQVLLHELDLCENGIVLGVDSRKKINSYVEKSLTEDETDTDQIISSRLVVFRNIRELQQQNQNLLSAVHELADRMEKDEKPDLDGAEIQEETLIKANETIDQLTKMLEEVSDQLRYSLKERDFFRSLVQENEKLLDMAPATPNSKLNTNLIEQTSYQRSLIRLEQLTNELESLKSISRNKEKKFEEAISSLQLEKSNIQLQLTSLTSERSLALEKLNDLEKSLVLSERSKDELDESYKSLQEQLASKKIEVQNVSSQLSICNSQLEQSNHIVDNLKSENLLLTSVKDKLKADLSNLESKLSSLQQDNFHMKAQIESSNQEYTATVDSMNSRILELSNDLRVANSKLSECSDDVRRLTLQNSFDLREHQTLVLQLQSNITELKQDITLQRTVRNQLEIQTTELKERLKFMEERQENLQSKLIAANKDTTQNPDNVEVEAISIELERTKEKLRMAELEKSNIQQKYLASEKTLEMMNETHEQFKHLVESEISTREEKITSLRSELLDLNKRVEVLKEEKESSSKELAKQLEDAVREKDSALSFKKDYEKIRSDADRVITSLKEDIEKERSLMKECHSNYESEIVSHGRTTQKLRDLRTEFDEVNTKYLKLKANFEQQHSGLSGAEKDWNIQRKAMEDEISSLKDYILGLENQNKLLHSQFDSLSQQITVLQQNSSENLNISANLEAVQDNDLRELVSYLRHEKEIMDNKYELTILDNRGLNQQVKSLQSTVDSLQLELNRLQSLPVSNDQTDTPIISGSQEVQLLYESNSVLRKDNDAKLGKIQELEKEVEKLNASLNPLQTEINELKAEIGAKTASLNLMKEYNSRWKLRFQSVLNKYERVDPTQLEELKKNCEALEKEKQELETKLQETAKETDTFKQQVNSLNEEVENLKKEVEQANTKNTRLAAAWNEKCENLKKSSLTRFAHLKQELTNKNKELTSKNAENEAMQKEIESLKDSNHQLQESASSDAEQITKEQFEQLKSEKERTEKELADSKNELEHLQSEAVDADGKTEISNLEKEIHELRSDKEGLVQQVQNLSAELAALREHSPTQGSLENADEIARLRSQLESTKQYYEKEKETEILAARSELVAEKEKTKEELENQLNEKSQRIKELEEQAQKNSSENTHDNIDDMIKQQVEEKLKENSANFDVKLKKVVAETEFRSKAKISVYEKKTRDLQNKITQLEETIENLNKQLSNPEKTDESTSSVTETKPVTSKPTASKADVGQNATEASSAKREPSGKSLSARLQGTGKQKGVQRPAVSRPVPMKPDSGKLSITGASKRIATSKNAAQNAKELSSTAKSGSLKRQRDDANKGGSSSNQKKAK

Target ORF information:

RefSeq Version	NM_001023226.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe nucleoporin nup211 (nup211), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023226.2

ORF Insert Sequence:

ATGCACGATT CTTCCTGGAC AGAAGCTGAT ATACTCGGGG TATGCAGTTT TCTAGACATA 
CCAAAGACAA AAATTGATCC TTTACTTCAG GTGGACGCGT TTACATCCAT ATTAATACCT 
TTAATTAGTA AAAGCAAAGA TTATGAAAGT ATAAAAAATG ATCGTATAGT TACAGAAGTC 
AATTACGAGC AACAATTGCG AAATTCGGAG AAGAAACTAC TTCAATCAAA CGAACGTTAC 
GATTTATTAG AGGATGAGCG CAAGCTTTTA GAGAATGAAC TCTCTCAAAT CAAAGAATAT 
CTTAGGGAGA AATCCTCTTC ATATGACACT GTTTTGCACG ATTGCAGTTC TCTCAAAAGT 
GTAAATGAGG CATTAAAACA AGCTCAGGAT CAAAATTTGA AGCAAACTGC TCAACTTCAA 
AATCTTTTAT CAGATAAAGA AAAGGAAGTT GAAAAGAAAA TCACTATCAT TAAAGATCTA 
AAGGATGCCC TTGCCAGTTC TACCCATCAA GTTTTGGAGC TTCAACATAC ACAACAAGAA 
AAAGCATCTT TGCAGACTAA CTATGAGTTT GAGTTGCAAA AATTAACGCA GAAAAATTCG 
ATTTTGGAAA ATAATAACAC CTGGTTAAGC CGAGAGCTTC AGGGTGTGAA CGACAAATTG 
TTATCTCTTC ATCAGGAGGC CTCTTTGGAA AAGTCTCAAC TAAGTTCTCA ATTATCTGAC 
GCTGTTTTGG AAAAAGATGC GCTACAACGG AAAGTGAGTT CTTTGTCTCA ACAGTTTACA 
GAAAGTAATT TACGATATCA GAATATTGTA GCTGAACTCT CCGAAATGCG AAAGCAATAT 
GAGTTTTCGC AAGTTTCTTT TGAAAAAGAG ATTTCTTCAC AAAAGCAAAT TAGTGAGTTG 
TGGATGGAGA AATGTGAGGA TTGCTCTTTA CGGCTCAAAG AGTTGCAGAA CTCAAACGGC 
GAGTTGGAAA AGCTATTGGA AGCTGCACAA TCATCTTTTG AAGAACAGCT TGAATCTCAT 
AAGGAAGCAG AAGCTTCCTT GAAATCTCAA ATCAATTTTC TAGAGAAAGA GGTATCAAGT 
TTAGAGTCTC AGCTAAAGTT GGCTAATGAG CGTCTTCGGC ATTATGATGA AATAGAAATC 
TCAGATATGA GTGAATTGAA ATACTCAAAT TTACTAAATA ATAGTATGAA AGGCTTTAAA 
GGTCAGTCCT CCGTCTCTGA CTTGTATTCC GAGCGTTTAT ACTATAAACA GAAGTATGAG 
CAAACATGTC AAGAGGTTGA AAGGTTACAA CGTAGCTATA ACCATGTTAT GGAAGAAGCG 
AATCTTCAGC ATCCTTTGGT TAAAGAACAG TTTAAGCGAT TTGCTCATAT GCAACGGGAA 
ATTGTTGCAA TGAGTGAGCA ATATCAGAAG TCATTGGAAG ACTGTCAGAA AGCCAAGTCC 
CGTTACGAGC AATTAGAGAC ACTATTTAAA GATAAATGCA CAGAGAATAA GCATTATGAG 
CAAGAGACCA AAGATTTAGC GCGACAGGTG CAAGTTTTAC TTCATGAACT CGATTTGTGT 
GAGAACGGCA TAGTGCTAGG CGTGGATAGT AGAAAGAAGA TTAACTCCTA TGTTGAAAAA 
TCGCTCACTG AAGATGAAAC TGATACGGAT CAAATAATTT CAAGCAGGCT AGTTGTATTT 
CGTAATATTA GAGAATTGCA ACAACAAAAT CAAAATCTAC TGTCTGCAGT TCATGAATTA 
GCTGATCGTA TGGAGAAAGA CGAAAAACCT GATCTTGATG GAGCCGAGAT TCAAGAAGAA 
ACACTGATAA AAGCTAACGA AACTATTGAT CAACTTACTA AAATGTTGGA GGAGGTGAGC 
GATCAGTTAC GCTACTCTTT AAAGGAACGA GACTTTTTTA GATCACTAGT TCAAGAGAAT 
GAAAAACTTT TAGATATGGC GCCTGCAACT CCTAATTCCA AATTGAATAC TAATTTAATT 
GAGCAGACTT CTTACCAGCG TTCGCTTATT CGACTTGAAC AGCTAACTAA TGAACTGGAG 
TCCCTTAAGT CAATTTCTCG TAACAAAGAA AAAAAGTTTG AGGAAGCTAT CTCATCTCTC 
CAGCTCGAAA AATCTAATAT TCAATTGCAA CTTACCTCTT TAACATCTGA ACGAAGCTTA 
GCCCTTGAAA AGCTAAATGA TCTGGAAAAA TCACTTGTTT TATCTGAAAG AAGTAAAGAT 
GAACTTGATG AAAGCTATAA GTCTTTACAA GAGCAACTTG CTTCTAAGAA AATTGAAGTT 
CAAAATGTTT CTTCTCAATT ATCAATCTGT AATAGCCAGC TTGAACAATC CAATCATATT 
GTTGATAATT TAAAAAGCGA AAACCTGCTT TTAACTTCCG TCAAGGATAA GTTGAAAGCC 
GATTTATCTA ATTTGGAGTC GAAACTTTCG TCCCTGCAGC AGGATAATTT CCATATGAAA 
GCTCAAATTG AATCTTCAAA TCAAGAGTAT ACGGCAACAG TGGACTCAAT GAATAGTCGA 
ATACTTGAAC TTTCTAATGA TCTTCGAGTT GCTAATTCAA AGCTATCTGA GTGTTCCGAT 
GATGTTCGTA GACTTACTCT ACAAAATTCA TTTGATTTGA GGGAGCATCA AACATTGGTG 
CTTCAACTTC AGTCTAACAT CACTGAGTTA AAACAAGACA TAACATTGCA ACGCACCGTG 
CGAAATCAGT TAGAAATACA AACCACTGAG TTGAAAGAGC GCTTAAAGTT TATGGAAGAA 
CGGCAGGAGA ACTTGCAATC TAAATTAATC GCGGCTAATA AAGACACGAC ACAAAATCCT 
GATAATGTTG AAGTCGAGGC TATATCTATT GAACTGGAAA GGACGAAGGA AAAACTTCGC 
ATGGCCGAGC TTGAAAAGAG TAATATTCAA CAAAAATACC TCGCTTCAGA AAAAACCTTG 
GAAATGATGA ATGAAACCCA TGAGCAGTTT AAGCATCTAG TAGAATCTGA AATTAGCACA 
AGGGAAGAGA AAATTACGTC CCTTCGCTCT GAGTTGTTGG ATCTTAATAA GAGAGTTGAA 
GTACTAAAAG AAGAGAAAGA GTCTTCGTCC AAGGAACTTG CCAAACAACT TGAGGATGCT 
GTTCGTGAAA AGGATAGTGC ACTTTCTTTC AAAAAAGATT ATGAAAAAAT TCGTTCAGAT 
GCGGATCGTG TTATTACCTC TTTGAAAGAA GATATTGAAA AAGAAAGATC TCTAATGAAG 
GAGTGTCATT CTAATTACGA ATCAGAGATC GTTAGTCATG GGCGTACCAC TCAAAAATTG 
CGGGATTTAC GTACTGAATT TGACGAGGTG AATACGAAAT ATTTAAAGCT CAAAGCAAAT 
TTTGAACAGC AGCACTCAGG ACTTTCAGGT GCTGAAAAGG ATTGGAATAT TCAGCGGAAA 
GCCATGGAAG ATGAAATTAG CTCCTTGAAA GATTACATTT TGGGACTTGA AAATCAAAAT 
AAACTACTAC ATTCGCAATT TGATTCTCTT AGTCAACAAA TAACTGTTCT TCAACAGAAT 
TCTTCTGAAA ATTTAAATAT ATCTGCCAAT TTAGAAGCCG TTCAAGATAA CGATTTACGT 
GAACTTGTTA GCTACTTGAG GCACGAAAAG GAAATAATGG ATAATAAATA TGAGTTAACA 
ATTCTGGATA ATAGAGGTTT GAATCAACAA GTCAAGTCTT TGCAATCCAC CGTTGATTCA 
TTGCAACTCG AGCTCAACCG TCTGCAGTCT CTGCCTGTGT CAAATGACCA AACAGATACC 
CCCATTATCA GCGGAAGTCA AGAAGTTCAG TTACTTTATG AAAGTAATAG TGTCCTTAGA 
AAGGACAATG ATGCTAAACT TGGAAAAATA CAAGAGCTGG AGAAAGAGGT TGAAAAACTG 
AATGCTTCAT TGAATCCCTT ACAAACTGAA ATAAATGAAC TTAAGGCAGA AATAGGCGCA 
AAGACAGCAA GTCTGAACTT AATGAAGGAA TACAATTCTC GATGGAAATT AAGATTTCAG 
TCCGTTTTGA ACAAGTATGA GCGTGTTGAT CCCACACAGT TAGAGGAGTT AAAGAAAAAT 
TGTGAAGCAT TAGAAAAAGA AAAACAAGAG TTGGAAACGA AACTTCAGGA GACAGCAAAA 
GAAACCGATA CATTTAAGCA ACAGGTTAAT TCTCTAAACG AAGAGGTGGA AAATTTGAAG 
AAGGAAGTTG AGCAGGCAAA CACCAAAAAT ACTAGATTGG CAGCTGCTTG GAATGAAAAA 
TGTGAAAATT TGAAAAAATC CAGCTTAACA AGATTTGCCC ATCTAAAGCA GGAATTGACC 
AACAAAAACA AGGAGTTAAC CTCTAAGAAT GCTGAAAATG AAGCAATGCA AAAAGAAATA 
GAGTCTCTAA AGGATTCCAA TCATCAATTA CAAGAATCGG CTTCCAGTGA TGCTGAACAG 
ATCACGAAGG AACAATTTGA ACAACTCAAG TCGGAGAAGG AAAGAACTGA AAAAGAGCTA 
GCAGATTCTA AGAACGAGCT AGAACACTTG CAATCTGAGG CTGTCGATGC TGACGGTAAA 
ACTGAAATTT CAAATCTTGA AAAAGAAATT CATGAATTAC GGTCTGACAA AGAAGGACTT 
GTGCAACAAG TACAAAACTT GAGTGCGGAA TTGGCAGCTT TACGAGAGCA TTCACCGACC 
CAAGGAAGTT TAGAGAATGC TGATGAGATT GCAAGGCTAC GTTCACAACT TGAATCTACG 
AAACAATATT ATGAAAAAGA AAAGGAAACC GAAATTTTGG CTGCTCGCTC TGAACTCGTC 
GCAGAGAAAG AAAAGACAAA GGAAGAGTTA GAAAATCAAC TCAACGAAAA GTCACAGAGA 
ATAAAGGAAC TTGAGGAACA AGCTCAAAAA AATTCTTCGG AAAATACTCA TGACAATATT 
GATGACATGA TTAAGCAGCA GGTTGAAGAA AAGCTTAAAG AAAATTCTGC CAACTTTGAT 
GTTAAGTTGA AGAAGGTGGT AGCTGAGACA GAATTTCGGA GCAAAGCTAA AATATCTGTG 
TATGAGAAGA AAACTAGGGA TCTTCAGAAC AAAATTACAC AACTTGAAGA AACTATTGAG 
AATTTGAATA AACAGCTATC CAATCCTGAA AAGACTGATG AATCTACGTC TTCTGTAACA 
GAAACAAAGC CTGTTACTTC CAAACCAACA GCTAGCAAAG CTGATGTTGG TCAGAATGCT 
ACAGAAGCTT CGTCAGCCAA GAGAGAACCT TCCGGTAAAT CTCTGAGTGC AAGACTTCAA 
GGAACAGGCA AACAAAAAGG TGTTCAACGA CCCGCTGTTT CGCGTCCTGT TCCTATGAAA 
CCAGACAGTG GAAAACTCTC AATAACTGGA GCTTCCAAAC GAATTGCTAC TTCAAAGAAT 
GCTGCCCAAA ACGCTAAAGA ACTGTCAAGC ACTGCTAAAT CCGGCTCCCT TAAAAGACAA 
CGTGACGATG CGAACAAAGG AGGATCCAGT TCGAACCAAA AGAAAGCAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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nup211 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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