btb1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following btb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the btb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99441 NM_001022706.1
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Schizosaccharomyces pombe BTB/POZ domain protein Btb1 (btb1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99441
    Clone ID Related Accession (Same CDS sequence) NM_001022706.1
    Accession Version NM_001022706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product BTB/POZ domain protein Btb1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTCATC TGCTTTTCGC GTATTACCTT TGCAACGACA TTCGCTCATT CCAAAATCTA 
    CTGAAGCAAG ATGATTCAAA GGTGAAAGAG CCTAGAAAGG GTTTTTCGGA AAAGAGCGGA
    CAAAAACTCC GTATCAACCA AAAAGATCGA TACGGACGCA CCGTGCTGCA CATCGCCGTT
    TCTGAGAATA AAAATTCCTT TGTTAGATCT CTTTTGCAGC ATAAAGGCAT TGATGTTTTT
    GTGCAAGATG AAGAATCTGG ATATACGGCG TTGCATCGGG CAATCTACGT TGGCAACTTG
    GAAGCAGCGT CTTTGCTTTT ATCAAAAGAT CCCTCCTTCA GATCATTGCG TATTAAAGAC
    AAAGAAGGTC TTTCTCCATT TCAATTTTTA TCACGAGTCC TTTCAAGCAC TATTCATCCG
    GTTCTTGACT TACCGATAAT AGGAAATGAA CTTTATGGCT TTGGTACTAA CGTAAACAAT
    ACGCTCGGAA TAGCAAATGG GAAAGAACCT TCATCGCCAG AAAGAGTGTT TTTACTGAAA
    AACCAAACAG AGTCTCCCAC ATCAGGTCAA CTCTTTTCCC GAGACAAAAT CCTTGATGTG
    CAGGCTTCCA AATTTCACAG CGTCGTACTA ACGGATGAGC CGTCGCAAAA TGTTTACGTA
    TGTGGTATTG GTGCTGGAGG GAGAATAGGT TTTAACACGG ACGTTCAATA TAACTTTATT
    CCTATTCCTG GTATCATTCA TAAAGTTATC CAGATTTCTG TTAGTCATAC CCATTCACTT
    GCTCTTACAA AGTTCGGAAG TATTTATTCG TGGGGAAAGA ATGGTTCTGG TGAACTGGGA
    CTTTCTAATG ACGAACTGAA AAAGGATGAT CCAATTCAAA TTACCCCTAG GCGTATCTCT
    GCATTCAAGG ATTATCAGAT AATTGGAATG GCGGCTGGAA AAAGTTATAG CGTTGCTTGG
    ACTGACACTG ATATTTACAG CTGGGGGTTA AATAATGGCC AATTAGGTAT ATCTGATCAT
    ATTTCAGTTG TTTCTACTCC AAGGAGAGTT GCTGGCTTAT TATCGCCTGT CATACATGCC
    GTTTGTACTA CTCGTGCTAC GATATGCCTT TTACAAAACA ACAGTATAAT TGCCTTTTGC
    AATTATAATC AAGTGAAATT GCCATTCAAC GTTGACTTTG GTAGTTCGTT AAAGGTAACT
    AAGCATCCTC TTTCCCTCAC CAGATTTAAC GTTAGAAAAT TATTAGCTTC AGAAAACAAA
    CTAGCCGTGC TTACAGAACT AGGCGAAGTC TATGAATTTG ATATGAAGCT TTTACTTGAT
    CGTGATTCTA CCTCTTCCAA GAATTCCACA CGAACTTCAT TCAAATTTAC TCCATTATGG
    ATTTTTGAAT CTTCAGACCT TGCAGCTTTA GATATTGCGT GGACCGCTGA TAATTCTTTA
    ATACTATGTA CTAGAAATGG CACTTGTTGG AAACGGGAGT TAAGGTCAAA AAAGCGAGAA
    AAGTCATCTA GTAGCCCGTA TTCTAGAGGA CCATACAAAT ATAATAGAAT TGAAAATTTA
    CAAATGGTTG TTGGAGTCCG TGCGAGCGCT TCTGGATCAT TTTTTGCAAT TCGAAATGAT
    TATCTTCCAC CGCCAATCTA CAAGCCTTCG AATATGTTAA TTGATCTTTT GCGCTCACTA
    CTTCCCTATG ACCACCTTTT ACATGTAAGA CAACCACGTT TGATTCCTCC CGAAGATGAA
    GACGGAGTCC CTATTTTTGA CGAAGACCGC GCTGCATCCA GCAATGAGAT GCAGCTACTT
    TTTGAAGGAT CTATACCGAT TCTTACTAGT TATGAAAATT ATAAGCAATC ATTTTCTGAT
    GTTACTATTT ATTGTGGTAC TTCTATGTTC CACTCACATA AGTTCATATT GTGTGCTCGA
    TCTTCATTTT TGAGAAAATT ATTGCTACAA AAAAAGAAAT CGAGTGTCTC CAACATCATT
    TACATTGAAG AAATCACTCA ATCTCATTCA ACAATTAGAG TTGAAGATAT TCCTCCTTTA
    GCGGTTGCTA TTCTTTTGCA TTATTTGTAC ACGGATACAT TGCTTTCTCC ATGGCATCTT
    GATTCACGCT TTTCACCTTT AAAAGAAAAC CTTTCAAAAC TTGCAAACTT ACTGGAGTTA
    CCTCATTTAG CGGAGGTTTT ACCGTTTAGT GTTTCCCGCC AACCTTTGCT TTCTTTAACG
    AATGACATTC TGCAACTATA TAATAATTTT TATGTCTTAT GCGAAGAAAC AATGGATACA
    GTAATAAAGC TTAAAGATGG AGAGCTCAAG GCACATGGTC TTTTTTTAAG TCTCCGCAGT
    GAATACTTTA GTTCTTATTT CCAATTTGTT TCTATGGAAA GCAATAGTTT TGACATCCCA
    ATAACCGTCA ATTTGTCTCA TTTGACTGTT GAGCATATGT CAATTGTTCT TCGTCATGTA
    TACAGTGACT TAAAAGTTGA GCTTTTTGAC GATTTGAAAG AATCCGATTT TCATAATTGG
    TTGGAAACTA TGTTCGAAAT TTTGTCTATT GCAGACGAGC TTCTTTTTCT TGAACTAAAA
    AGCATAGCCC AGCAATCCTT ACTTCGCTTT TTAAATTTGA AAACTCTACC TACTCTAATG
    GATCTTTCAC TTAGTTATCA CGCTGAGGAG CTTTATTCAC GATGTATTGA TTATGCTTGT
    CACAATATTG AATTCTTTTT GGAAGCAAAT CGTATAAGTG AATGGGATGG TTTCCATTTA
    AAAAAAGTCG CTCAACGTTT AACTGAATTG CTTTCTGATC AACGAGTACA TCTTCCTTCT
    AGCAAGATTG CTAATAGATT ATTGATAAGA GATCCAGTGT TGATGGAAAA AAGAAATTAT
    GAGCTTAAAG TTCTCAGAGA ATACTTGTTT TCCCAGGAAA GTTCTCAATT ATGGGACGAT
    TCCCCTTATC GGTCTATTTT TGAAGATCGT CGATGCAGCA CTTCAGCAGT AATTTTAGAA
    TCTGGAATTG TTCCTTCAAG TAATCAAAGT GATTCATTAA ATAAGGAAGA TGCTGAGGAG
    AAGTCTCCTA AACCAAATGT TGTTAACGTT ACGAGCATTA CAAAGACAGC AGGAGCTTCT
    GTTGAAATTC AAAACAATAT AGAGTCTGCT TCGTCAGGAG GGGATAAAAC ACAATTAAAT
    GGTCCTGGGG CTGATCAACC TGTTACTGCA ACAATTACTT TTGACAAAAC GAGTCCATGG
    AGGAATAGGG AAAATCTTTC TCATAATAAC AACACTACTA GAGCTAGTCT TAGAGAATTG
    CTGGAGCAAG AGAAAGCTGA TGCTTCAACT ACTACGGTTT TGTCTGATTC TAGATTTATG
    AAAGCTCCAA CTAAAAAATC CCAACGTGAG AAAAAAAAGG AGCTTTCAAA GCAAGTGCCT
    ATTTCCAAAA CAAATGTTGG CCACATCGAT ATCGAGTTGG GTAAATCGAA TCACTCAAAC
    CCTTGGTCTG TAGCAACACA TCAGAGGGGC AGTTTTTCGT CATCAACTGG AGTGAAAAAG
    TCATTTAATG GTATTCTTAG AGAGGCAGCT CGAGAGGAAG GTTCATCTCA AGTAATTTAT
    CAAGAATCGA AGAAGCGCAT TTCCAATGGT TCTCCAACTA GTTGGAACCT ACTTACTAAA
    CCATCTCCCC GATCTGCTTC TTTACCAAAA AATAGCCAAC CTTTATCGAT TTCGGAGATT
    ATGACTGAGC AGAAAGAAGA AATTGAGAGT CAAAAGAGGA GATCATCTTT TAGAAAAACA
    ATTGAGGAGA TACAACAGGA AGAGGAGTTT CAAAAGTGGT GGGAGGAGGA GAGTTTGAGA
    GTACAGAAAG AACTTGGAAT CCTTAAAACT GAAAGAGATA CCTCTACGAA TAGAAAACAG
    GGACAAGCTT CTAAACAACC TCAACGAAGG CACAGAAAGG AAAAAGATTC CAAAGTGTCA
    GAGTCCACTG CGGAGTTTAA ATCTCTTCCT ATTGATATAC CAAGGACTAC TCACAAGAAA
    GGAAAAGCAC GTGCAGTCAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587711.1
    CDS1..4044
    Translation

    Target ORF information:

    RefSeq Version NM_001022706.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe BTB/POZ domain protein Btb1 (btb1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTCATC TGCTTTTCGC GTATTACCTT TGCAACGACA TTCGCTCATT CCAAAATCTA 
    CTGAAGCAAG ATGATTCAAA GGTGAAAGAG CCTAGAAAGG GTTTTTCGGA AAAGAGCGGA
    CAAAAACTCC GTATCAACCA AAAAGATCGA TACGGACGCA CCGTGCTGCA CATCGCCGTT
    TCTGAGAATA AAAATTCCTT TGTTAGATCT CTTTTGCAGC ATAAAGGCAT TGATGTTTTT
    GTGCAAGATG AAGAATCTGG ATATACGGCG TTGCATCGGG CAATCTACGT TGGCAACTTG
    GAAGCAGCGT CTTTGCTTTT ATCAAAAGAT CCCTCCTTCA GATCATTGCG TATTAAAGAC
    AAAGAAGGTC TTTCTCCATT TCAATTTTTA TCACGAGTCC TTTCAAGCAC TATTCATCCG
    GTTCTTGACT TACCGATAAT AGGAAATGAA CTTTATGGCT TTGGTACTAA CGTAAACAAT
    ACGCTCGGAA TAGCAAATGG GAAAGAACCT TCATCGCCAG AAAGAGTGTT TTTACTGAAA
    AACCAAACAG AGTCTCCCAC ATCAGGTCAA CTCTTTTCCC GAGACAAAAT CCTTGATGTG
    CAGGCTTCCA AATTTCACAG CGTCGTACTA ACGGATGAGC CGTCGCAAAA TGTTTACGTA
    TGTGGTATTG GTGCTGGAGG GAGAATAGGT TTTAACACGG ACGTTCAATA TAACTTTATT
    CCTATTCCTG GTATCATTCA TAAAGTTATC CAGATTTCTG TTAGTCATAC CCATTCACTT
    GCTCTTACAA AGTTCGGAAG TATTTATTCG TGGGGAAAGA ATGGTTCTGG TGAACTGGGA
    CTTTCTAATG ACGAACTGAA AAAGGATGAT CCAATTCAAA TTACCCCTAG GCGTATCTCT
    GCATTCAAGG ATTATCAGAT AATTGGAATG GCGGCTGGAA AAAGTTATAG CGTTGCTTGG
    ACTGACACTG ATATTTACAG CTGGGGGTTA AATAATGGCC AATTAGGTAT ATCTGATCAT
    ATTTCAGTTG TTTCTACTCC AAGGAGAGTT GCTGGCTTAT TATCGCCTGT CATACATGCC
    GTTTGTACTA CTCGTGCTAC GATATGCCTT TTACAAAACA ACAGTATAAT TGCCTTTTGC
    AATTATAATC AAGTGAAATT GCCATTCAAC GTTGACTTTG GTAGTTCGTT AAAGGTAACT
    AAGCATCCTC TTTCCCTCAC CAGATTTAAC GTTAGAAAAT TATTAGCTTC AGAAAACAAA
    CTAGCCGTGC TTACAGAACT AGGCGAAGTC TATGAATTTG ATATGAAGCT TTTACTTGAT
    CGTGATTCTA CCTCTTCCAA GAATTCCACA CGAACTTCAT TCAAATTTAC TCCATTATGG
    ATTTTTGAAT CTTCAGACCT TGCAGCTTTA GATATTGCGT GGACCGCTGA TAATTCTTTA
    ATACTATGTA CTAGAAATGG CACTTGTTGG AAACGGGAGT TAAGGTCAAA AAAGCGAGAA
    AAGTCATCTA GTAGCCCGTA TTCTAGAGGA CCATACAAAT ATAATAGAAT TGAAAATTTA
    CAAATGGTTG TTGGAGTCCG TGCGAGCGCT TCTGGATCAT TTTTTGCAAT TCGAAATGAT
    TATCTTCCAC CGCCAATCTA CAAGCCTTCG AATATGTTAA TTGATCTTTT GCGCTCACTA
    CTTCCCTATG ACCACCTTTT ACATGTAAGA CAACCACGTT TGATTCCTCC CGAAGATGAA
    GACGGAGTCC CTATTTTTGA CGAAGACCGC GCTGCATCCA GCAATGAGAT GCAGCTACTT
    TTTGAAGGAT CTATACCGAT TCTTACTAGT TATGAAAATT ATAAGCAATC ATTTTCTGAT
    GTTACTATTT ATTGTGGTAC TTCTATGTTC CACTCACATA AGTTCATATT GTGTGCTCGA
    TCTTCATTTT TGAGAAAATT ATTGCTACAA AAAAAGAAAT CGAGTGTCTC CAACATCATT
    TACATTGAAG AAATCACTCA ATCTCATTCA ACAATTAGAG TTGAAGATAT TCCTCCTTTA
    GCGGTTGCTA TTCTTTTGCA TTATTTGTAC ACGGATACAT TGCTTTCTCC ATGGCATCTT
    GATTCACGCT TTTCACCTTT AAAAGAAAAC CTTTCAAAAC TTGCAAACTT ACTGGAGTTA
    CCTCATTTAG CGGAGGTTTT ACCGTTTAGT GTTTCCCGCC AACCTTTGCT TTCTTTAACG
    AATGACATTC TGCAACTATA TAATAATTTT TATGTCTTAT GCGAAGAAAC AATGGATACA
    GTAATAAAGC TTAAAGATGG AGAGCTCAAG GCACATGGTC TTTTTTTAAG TCTCCGCAGT
    GAATACTTTA GTTCTTATTT CCAATTTGTT TCTATGGAAA GCAATAGTTT TGACATCCCA
    ATAACCGTCA ATTTGTCTCA TTTGACTGTT GAGCATATGT CAATTGTTCT TCGTCATGTA
    TACAGTGACT TAAAAGTTGA GCTTTTTGAC GATTTGAAAG AATCCGATTT TCATAATTGG
    TTGGAAACTA TGTTCGAAAT TTTGTCTATT GCAGACGAGC TTCTTTTTCT TGAACTAAAA
    AGCATAGCCC AGCAATCCTT ACTTCGCTTT TTAAATTTGA AAACTCTACC TACTCTAATG
    GATCTTTCAC TTAGTTATCA CGCTGAGGAG CTTTATTCAC GATGTATTGA TTATGCTTGT
    CACAATATTG AATTCTTTTT GGAAGCAAAT CGTATAAGTG AATGGGATGG TTTCCATTTA
    AAAAAAGTCG CTCAACGTTT AACTGAATTG CTTTCTGATC AACGAGTACA TCTTCCTTCT
    AGCAAGATTG CTAATAGATT ATTGATAAGA GATCCAGTGT TGATGGAAAA AAGAAATTAT
    GAGCTTAAAG TTCTCAGAGA ATACTTGTTT TCCCAGGAAA GTTCTCAATT ATGGGACGAT
    TCCCCTTATC GGTCTATTTT TGAAGATCGT CGATGCAGCA CTTCAGCAGT AATTTTAGAA
    TCTGGAATTG TTCCTTCAAG TAATCAAAGT GATTCATTAA ATAAGGAAGA TGCTGAGGAG
    AAGTCTCCTA AACCAAATGT TGTTAACGTT ACGAGCATTA CAAAGACAGC AGGAGCTTCT
    GTTGAAATTC AAAACAATAT AGAGTCTGCT TCGTCAGGAG GGGATAAAAC ACAATTAAAT
    GGTCCTGGGG CTGATCAACC TGTTACTGCA ACAATTACTT TTGACAAAAC GAGTCCATGG
    AGGAATAGGG AAAATCTTTC TCATAATAAC AACACTACTA GAGCTAGTCT TAGAGAATTG
    CTGGAGCAAG AGAAAGCTGA TGCTTCAACT ACTACGGTTT TGTCTGATTC TAGATTTATG
    AAAGCTCCAA CTAAAAAATC CCAACGTGAG AAAAAAAAGG AGCTTTCAAA GCAAGTGCCT
    ATTTCCAAAA CAAATGTTGG CCACATCGAT ATCGAGTTGG GTAAATCGAA TCACTCAAAC
    CCTTGGTCTG TAGCAACACA TCAGAGGGGC AGTTTTTCGT CATCAACTGG AGTGAAAAAG
    TCATTTAATG GTATTCTTAG AGAGGCAGCT CGAGAGGAAG GTTCATCTCA AGTAATTTAT
    CAAGAATCGA AGAAGCGCAT TTCCAATGGT TCTCCAACTA GTTGGAACCT ACTTACTAAA
    CCATCTCCCC GATCTGCTTC TTTACCAAAA AATAGCCAAC CTTTATCGAT TTCGGAGATT
    ATGACTGAGC AGAAAGAAGA AATTGAGAGT CAAAAGAGGA GATCATCTTT TAGAAAAACA
    ATTGAGGAGA TACAACAGGA AGAGGAGTTT CAAAAGTGGT GGGAGGAGGA GAGTTTGAGA
    GTACAGAAAG AACTTGGAAT CCTTAAAACT GAAAGAGATA CCTCTACGAA TAGAAAACAG
    GGACAAGCTT CTAAACAACC TCAACGAAGG CACAGAAAGG AAAAAGATTC CAAAGTGTCA
    GAGTCCACTG CGGAGTTTAA ATCTCTTCCT ATTGATATAC CAAGGACTAC TCACAAGAAA
    GGAAAAGCAC GTGCAGTCAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L