SPCC594.01 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPCC594.01
Entrez Gene ID	2538708
Full Name	DUF1769 family protein
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022780.2	NP_587787.2	DUF1769 family protein
NM_001355873.1	NP_001342730.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPCC594.01 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPCC594.01 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj00173	NM_001022780.2 Latest version!	Schizosaccharomyces pombe 972h- DUF1769 family protein (SPCC594.01), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSc99018	NM_001355873.1 Latest version!	Schizosaccharomyces pombe uncharacterized protein (SPCC594.01), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPCC594.01 ( NM_001022780.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587787.2 Schizosaccharomyces pombe 972h- DUF1769 family protein (SPCC594.01), mRNA.

Price & Availability↑

CloneID	OSj00173
Clone ID Related Accession (Same CDS sequence)	NM_001022780.2
Accession Version	NM_001022780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	DUF1769 family protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022780.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAAATTAA AGGTCAGTAT TGGCTGCGAT GCACAGCACA CACAGATTGC TTGGGTGAAT 
TATGAAGGGC GGCCTACTGA AGTCGATGGC CCGTTATGGG TTGGTAGAAT CCTCGTTCGC 
GTACGCGATT TCGATGGTTT TACTCCTGAC GGTAGCCCTC CAAAGAGAGA TTCCGAGTAT 
TTTCGAGGTC GCTCACGAAA GTTTCAAATA CAAGCTGAAG GCCGATTCAA AAAGGAATAC 
AATGGAGATC AAGTCATTTA TGGCACACAG TTCGACCATA TGATCTCTAC CTTCCCAGAA 
AGTGCTTTCC GAGCTGGTAT GCGGATTGCC AAATATATTG ACCCTGCTGT GTATTATGAT 
AAATATGCCA GATCCCCGTA TATCATGTCA CCATTCGTCG CTTGTGTGAA TACACTCTCT 
GCATGGCCGG CTCCATCGCG TCTTGAAGAT GCTGTTATTT CATTAGTGGA AGCTGATTCT 
CAGGAATCAG ACACTGAATC GTTACCTGAA ATTAACGACT CCTCGGATGT CAGTTTATCA 
GACCTTCCCT CTACCAACGT TACTCCCAAG AAAACCACGA CACAAATAGA TGTTCAAACA 
AATATTGTTA CTCCGCCAAT TACTGTTACA GCAGTACCCG ACTCTCCAAA TCCCCCTGCT 
GCTACCCCCG CTACCGTTGC AGATAATGAT GATCTTAGTA TAGTATCCTC TGATTCTGGA 
AATACCACTG CACCTCGAAA AAATAGACAC CGGTATTGGA GTTTTGCTGG ATTTAGCGAT 
AGCACACGGT TTTCACATTT AAACGTTCCG GCTGCAATAC CGGATGCTCC ATCAGTTAAG 
ACTGAAACTT CCCAAAACGT GTCTCAATTA TCTGTAAAAT CTGCAGCTAC TTCTCTAGCT 
CCTGTGGAGG ATGATGAAGA CGATGAGCTT TTATTACATC GTCACATTAC CAATAAACAT 
AAGGATTTTA ATTTACATGT CAAGCAGCTA GGATTGCTTC ACAAACCTAG TTTGGACCTG 
GAAGAGGGTT ATATAATGGA TGAGAATTAT GGAAAGGACG TGTACAAGCA TGGTATTTCC 
AATGTTCCTG ATGACGAAAA TGAAGATGAC TTGCAACGTT ACTTTACTGC TTTAGAAATG 
CAACAGGATC AAAAGGAACA GCATAAGAAA TCTAAAAACA AAGACCCGTT TCGTAAAATT 
ACACATCCTT CTTTACATCT TGGAAAATTT TCCACGCCCA AACTAATAAA ACGTATGTCC 
CTTCGCTCAA AAAAAAGTTT AAGAAACGAT TCGAAATCGG ATGACGTCGG TAATTCTACT 
CATCGATTTT CTACCGCTAG TGCAGCATCC ACGTCTGCTG TAAAAACTGA AAAAGAAAAG 
AAAATGTCGG CGCCACGTCG CTCTCTTGAT AAATTAATTC GCATAGGTTC TTTACACCGT 
CACCATCATC ATCATCACAA AACCGACCTA ATTGAATCTG ACTCTGGAAT TGAAGCCAGT 
GAAAGTAACC GTCGTAAATC TGACATTTTT TCTTTTTCTG GAAGAAACTC ATTTTCCGTT 
TCTCGTCCTT CCTCTTCTCA TTCCACGTTG TCATATGCCA ATGATTCAGC ATCATCTGCT 
GTTAACGTGG CTGGAGAGAC TGGGTCGTTA CCTCCGCTAC GTGAACAAAC ATCTATAACC 
AGCGGTGTCC CACCTTCTAA TAGGCTTAAA AAACACGTTT CAACACCTGA AAAGATTGTT 
GAGGAGCGAT CGATTGATGA AGTATCACAA TCTAATACTC CTTCTTCAAA ACAATTGCCT 
CAATCTGTTG ACGGAAAGAC TGTTACCGCG AATGCTAATA GTACCACCGT CGCAAAGCAA 
CCCACGTCTA ATGTGGCGTT GACGAGGCCA AAGCCCGTGC GGACGGCTAC TAGTCAATCA 
AAAATACCGA AACCTGTTAA ACACATCCCT TCTGATTCAA ATAATCTCGA TCCACAACTT 
GGACCTTGGA GATTCGCCAA TCCCAAAGTG GATCCTATAG AAGACAATTC GTTTATTTTT 
GGTGAACACA AGTCAGTAAA GGAAAGACGA AAGTACTTTT CAAGCTCCAA ATTTTGTCGA 
GAAAATTTCT TTTACGATAA AGATGTAGTT TATTGCATGA GTTTCTTTTC CCCTCATATG 
GATTTTAATA CCTTTAATTT GAATATCGGA CCCATTCGGC TCAATGTTTA TAAGCATTTG 
AACTCTGATG GTCATCAGCC AATCCGATAT ATGATGCGTG AAACCGATGA TGAAGATGCT 
GTTATGTTTG TTGTCGAGTT TGACCTCCTC GAAGATGATG ATGAGACAGT TCTTGCCGAG 
CAGGCTAAGG AGCGTGAAAG AAGAAAACAA ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_587787.2
CDS	2206..4581
Translation	MKLKVSIGCDAQHTQIAWVNYEGRPTEVDGPLWVGRILVRVRDFDGFTPDGSPPKRDSEYFRGRSRKFQIQAEGRFKKEYNGDQVIYGTQFDHMISTFPESAFRAGMRIAKYIDPAVYYDKYARSPYIMSPFVACVNTLSAWPAPSRLEDAVISLVEADSQESDTESLPEINDSSDVSLSDLPSTNVTPKKTTTQIDVQTNIVTPPITVTAVPDSPNPPAATPATVADNDDLSIVSSDSGNTTAPRKNRHRYWSFAGFSDSTRFSHLNVPAAIPDAPSVKTETSQNVSQLSVKSAATSLAPVEDDEDDELLLHRHITNKHKDFNLHVKQLGLLHKPSLDLEEGYIMDENYGKDVYKHGISNVPDDENEDDLQRYFTALEMQQDQKEQHKKSKNKDPFRKITHPSLHLGKFSTPKLIKRMSLRSKKSLRNDSKSDDVGNSTHRFSTASAASTSAVKTEKEKKMSAPRRSLDKLIRIGSLHRHHHHHHKTDLIESDSGIEASESNRRKSDIFSFSGRNSFSVSRPSSSHSTLSYANDSASSAVNVAGETGSLPPLREQTSITSGVPPSNRLKKHVSTPEKIVEERSIDEVSQSNTPSSKQLPQSVDGKTVTANANSTTVAKQPTSNVALTRPKPVRTATSQSKIPKPVKHIPSDSNNLDPQLGPWRFANPKVDPIEDNSFIFGEHKSVKERRKYFSSSKFCRENFFYDKDVVYCMSFFSPHMDFNTFNLNIGPIRLNVYKHLNSDGHQPIRYMMRETDDEDAVMFVVEFDLLEDDDETVLAEQAKERERRKQT

Target ORF information:

RefSeq Version	NM_001022780.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- DUF1769 family protein (SPCC594.01), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022780.2

ORF Insert Sequence:

ATGAAATTAA AGGTCAGTAT TGGCTGCGAT GCACAGCACA CACAGATTGC TTGGGTGAAT 
TATGAAGGGC GGCCTACTGA AGTCGATGGC CCGTTATGGG TTGGTAGAAT CCTCGTTCGC 
GTACGCGATT TCGATGGTTT TACTCCTGAC GGTAGCCCTC CAAAGAGAGA TTCCGAGTAT 
TTTCGAGGTC GCTCACGAAA GTTTCAAATA CAAGCTGAAG GCCGATTCAA AAAGGAATAC 
AATGGAGATC AAGTCATTTA TGGCACACAG TTCGACCATA TGATCTCTAC CTTCCCAGAA 
AGTGCTTTCC GAGCTGGTAT GCGGATTGCC AAATATATTG ACCCTGCTGT GTATTATGAT 
AAATATGCCA GATCCCCGTA TATCATGTCA CCATTCGTCG CTTGTGTGAA TACACTCTCT 
GCATGGCCGG CTCCATCGCG TCTTGAAGAT GCTGTTATTT CATTAGTGGA AGCTGATTCT 
CAGGAATCAG ACACTGAATC GTTACCTGAA ATTAACGACT CCTCGGATGT CAGTTTATCA 
GACCTTCCCT CTACCAACGT TACTCCCAAG AAAACCACGA CACAAATAGA TGTTCAAACA 
AATATTGTTA CTCCGCCAAT TACTGTTACA GCAGTACCCG ACTCTCCAAA TCCCCCTGCT 
GCTACCCCCG CTACCGTTGC AGATAATGAT GATCTTAGTA TAGTATCCTC TGATTCTGGA 
AATACCACTG CACCTCGAAA AAATAGACAC CGGTATTGGA GTTTTGCTGG ATTTAGCGAT 
AGCACACGGT TTTCACATTT AAACGTTCCG GCTGCAATAC CGGATGCTCC ATCAGTTAAG 
ACTGAAACTT CCCAAAACGT GTCTCAATTA TCTGTAAAAT CTGCAGCTAC TTCTCTAGCT 
CCTGTGGAGG ATGATGAAGA CGATGAGCTT TTATTACATC GTCACATTAC CAATAAACAT 
AAGGATTTTA ATTTACATGT CAAGCAGCTA GGATTGCTTC ACAAACCTAG TTTGGACCTG 
GAAGAGGGTT ATATAATGGA TGAGAATTAT GGAAAGGACG TGTACAAGCA TGGTATTTCC 
AATGTTCCTG ATGACGAAAA TGAAGATGAC TTGCAACGTT ACTTTACTGC TTTAGAAATG 
CAACAGGATC AAAAGGAACA GCATAAGAAA TCTAAAAACA AAGACCCGTT TCGTAAAATT 
ACACATCCTT CTTTACATCT TGGAAAATTT TCCACGCCCA AACTAATAAA ACGTATGTCC 
CTTCGCTCAA AAAAAAGTTT AAGAAACGAT TCGAAATCGG ATGACGTCGG TAATTCTACT 
CATCGATTTT CTACCGCTAG TGCAGCATCC ACGTCTGCTG TAAAAACTGA AAAAGAAAAG 
AAAATGTCGG CGCCACGTCG CTCTCTTGAT AAATTAATTC GCATAGGTTC TTTACACCGT 
CACCATCATC ATCATCACAA AACCGACCTA ATTGAATCTG ACTCTGGAAT TGAAGCCAGT 
GAAAGTAACC GTCGTAAATC TGACATTTTT TCTTTTTCTG GAAGAAACTC ATTTTCCGTT 
TCTCGTCCTT CCTCTTCTCA TTCCACGTTG TCATATGCCA ATGATTCAGC ATCATCTGCT 
GTTAACGTGG CTGGAGAGAC TGGGTCGTTA CCTCCGCTAC GTGAACAAAC ATCTATAACC 
AGCGGTGTCC CACCTTCTAA TAGGCTTAAA AAACACGTTT CAACACCTGA AAAGATTGTT 
GAGGAGCGAT CGATTGATGA AGTATCACAA TCTAATACTC CTTCTTCAAA ACAATTGCCT 
CAATCTGTTG ACGGAAAGAC TGTTACCGCG AATGCTAATA GTACCACCGT CGCAAAGCAA 
CCCACGTCTA ATGTGGCGTT GACGAGGCCA AAGCCCGTGC GGACGGCTAC TAGTCAATCA 
AAAATACCGA AACCTGTTAA ACACATCCCT TCTGATTCAA ATAATCTCGA TCCACAACTT 
GGACCTTGGA GATTCGCCAA TCCCAAAGTG GATCCTATAG AAGACAATTC GTTTATTTTT 
GGTGAACACA AGTCAGTAAA GGAAAGACGA AAGTACTTTT CAAGCTCCAA ATTTTGTCGA 
GAAAATTTCT TTTACGATAA AGATGTAGTT TATTGCATGA GTTTCTTTTC CCCTCATATG 
GATTTTAATA CCTTTAATTT GAATATCGGA CCCATTCGGC TCAATGTTTA TAAGCATTTG 
AACTCTGATG GTCATCAGCC AATCCGATAT ATGATGCGTG AAACCGATGA TGAAGATGCT 
GTTATGTTTG TTGTCGAGTT TGACCTCCTC GAAGATGATG ATGAGACAGT TCTTGCCGAG 
CAGGCTAAGG AGCGTGAAAG AAGAAAACAA ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPCC594.01 ( NM_001355873.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342730.1 Schizosaccharomyces pombe uncharacterized protein (SPCC594.01), mRNA.

Price & Availability↑

CloneID	OSc99018
Clone ID Related Accession (Same CDS sequence)	NM_001355873.1
Accession Version	NM_001355873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-04
Organism	Schizosaccharomyces pombe(fission yeast)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421).

ATGAAATTAA AGGTCAGTAT TGGCTGCGAT GCACAGCACA CACAGATTGC TTGGGTGAAT 
TATGAAGGGC GGCCTACTGA AGTCGATGGC CCGTTATGGG TTGGTAGAAT CCTCGTTCGC 
GTACGCGATT TCGATGGTTT TACTCCTGAC GGTAGCCCTC CAAAGAGAGA TTCCGAGTAT 
TTTCGAGGTC GCTCACGAAA GTTTCAAATA CAAGCTGAAG GCCGATTCAA AAAGGAATAC 
AATGGAGATC AAGTCATTTA TGGCACACAG TTCGACCATA TGATCTCTAC CTTCCCAGAA 
AGTGCTTTCC GAGCTGGTAT GCGGATTGCC AAATATATTG ACCCTGCTGT GTATTATGAT 
AAATATGCCA GATCCCCGTA TATCATGTCA CCATTCGTCG CTTGTGTGAA TACACTCTCT 
GCATGGCCGG CTCCATCGCG TCTTGAAGAT GCTGTTATTT CATTAGTGGA AGCTGATTCT 
CAGGAATCAG ACACTGAATC GTTACCTGAA ATTAACGACT CCTCGGATGT CAGTTTATCA 
GACCTTCCCT CTACCAACGT TACTCCCAAG AAAACCACGA CACAAATAGA TGTTCAAACA 
AATATTGTTA CTCCGCCAAT TACTGTTACA GCAGTACCCG ACTCTCCAAA TCCCCCTGCT 
GCTACCCCCG CTACCGTTGC AGATAATGAT GATCTTAGTA TAGTATCCTC TGATTCTGGA 
AATACCACTG CACCTCGAAA AAATAGACAC CGGTATTGGA GTTTTGCTGG ATTTAGCGAT 
AGCACACGGT TTTCACATTT AAACGTTCCG GCTGCAATAC CGGATGCTCC ATCAGTTAAG 
ACTGAAACTT CCCAAAACGT GTCTCAATTA TCTGTAAAAT CTGCAGCTAC TTCTCTAGCT 
CCTGTGGAGG ATGATGAAGA CGATGAGCTT TTATTACATC GTCACATTAC CAATAAACAT 
AAGGATTTTA ATTTACATGT CAAGCAGCTA GGATTGCTTC ACAAACCTAG TTTGGACCTG 
GAAGAGGGTT ATATAATGGA TGAGAATTAT GGAAAGGACG TGTACAAGCA TGGTATTTCC 
AATGTTCCTG ATGACGAAAA TGAAGATGAC TTGCAACGTT ACTTTACTGC TTTAGAAATG 
CAACAGGATC AAAAGGAACA GCATAAGAAA TCTAAAAACA AAGACCCGTT TCGTAAAATT 
ACACATCCTT CTTTACATCT TGGAAAATTT TCCACGCCCA AACTAATAAA ACGTATGTCC 
CTTCGCTCAA AAAAAAGTTT AAGAAACGAT TCGAAATCGG ATGACGTCGG TAATTCTACT 
CATCGATTTT CTACCGCTAG TGCAGCATCC ACGTCTGCTG TAAAAACTGA AAAAGAAAAG 
AAAATGTCGG CGCCACGTCG CTCTCTTGAT AAATTAATTC GCATAGGTTC TTTACACCGT 
CACCATCATC ATCATCACAA AACCGACCTA ATTGAATCTG ACTCTGGAAT TGAAGCCAGT 
GAAAGTAACC GTCGTAAATC TGACATTTTT TCTTTTTCTG GAAGAAACTC ATTTTCCGTT 
TCTCGTCCTT CCTCTTCTCA TTCCACGTTG TCATATGCCA ATGATTCAGC ATCATCTGCT 
GTTAACGTGG CTGGAGAGAC TGGGTCGTTA CCTCCGCTAC GTGAACAAAC ATCTATAACC 
AGCGGTGTCC CACCTTCTAA TAGGCTTAAA AAACACGTTT CAACACCTGA AAAGATTGTT 
GAGGAGCGAT CGATTGATGA AGTATCACAA TCTAATACTC CTTCTTCAAA ACAATTGCCT 
CAATCTGTTG ACGGAAAGAC TGTTACCGCG AATGCTAATA GTACCACCGT CGCAAAGCAA 
CCCACGTCTA ATGTGGCGTT GACGAGGCCA AAGCCCGTGC GGACGGCTAC TAGTCAATCA 
AAAATACCGA AACCTGTTAA ACACATCCCT TCTGATTCAA ATAATCTCGA TCCACAACTT 
GGACCTTGGA GATTCGCCAA TCCCAAAGTG GATCCTATAG AAGACAATTC GTTTATTTTT 
GGTGAACACA AGTCAGTAAA GGAAAGACGA AAGTACTTTT CAAGCTCCAA ATTTTGTCGA 
GAAAATTTCT TTTACGATAA AGATGTAGTT TATTGCATGA GTTTCTTTTC CCCTCATATG 
GATTTTAATA CCTTTAATTT GAATATCGGA CCCATTCGGC TCAATGTTTA TAAGCATTTG 
AACTCTGATG GTCATCAGCC AATCCGATAT ATGATGCGTG AAACCGATGA TGAAGATGCT 
GTTATGTTTG TTGTCGAGTT TGACCTCCTC GAAGATGATG ATGAGACAGT TCTTGCCGAG 
CAGGCTAAGG AGCGTGAAAG AAGAAAACAA ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342730.1
CDS	2153..4528
Translation	MKLKVSIGCDAQHTQIAWVNYEGRPTEVDGPLWVGRILVRVRDFDGFTPDGSPPKRDSEYFRGRSRKFQIQAEGRFKKEYNGDQVIYGTQFDHMISTFPESAFRAGMRIAKYIDPAVYYDKYARSPYIMSPFVACVNTLSAWPAPSRLEDAVISLVEADSQESDTESLPEINDSSDVSLSDLPSTNVTPKKTTTQIDVQTNIVTPPITVTAVPDSPNPPAATPATVADNDDLSIVSSDSGNTTAPRKNRHRYWSFAGFSDSTRFSHLNVPAAIPDAPSVKTETSQNVSQLSVKSAATSLAPVEDDEDDELLLHRHITNKHKDFNLHVKQLGLLHKPSLDLEEGYIMDENYGKDVYKHGISNVPDDENEDDLQRYFTALEMQQDQKEQHKKSKNKDPFRKITHPSLHLGKFSTPKLIKRMSLRSKKSLRNDSKSDDVGNSTHRFSTASAASTSAVKTEKEKKMSAPRRSLDKLIRIGSLHRHHHHHHKTDLIESDSGIEASESNRRKSDIFSFSGRNSFSVSRPSSSHSTLSYANDSASSAVNVAGETGSLPPLREQTSITSGVPPSNRLKKHVSTPEKIVEERSIDEVSQSNTPSSKQLPQSVDGKTVTANANSTTVAKQPTSNVALTRPKPVRTATSQSKIPKPVKHIPSDSNNLDPQLGPWRFANPKVDPIEDNSFIFGEHKSVKERRKYFSSSKFCRENFFYDKDVVYCMSFFSPHMDFNTFNLNIGPIRLNVYKHLNSDGHQPIRYMMRETDDEDAVMFVVEFDLLEDDDETVLAEQAKERERRKQT

Target ORF information:

RefSeq Version	NM_001355873.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe uncharacterized protein (SPCC594.01), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355873.1

ORF Insert Sequence:

ATGAAATTAA AGGTCAGTAT TGGCTGCGAT GCACAGCACA CACAGATTGC TTGGGTGAAT 
TATGAAGGGC GGCCTACTGA AGTCGATGGC CCGTTATGGG TTGGTAGAAT CCTCGTTCGC 
GTACGCGATT TCGATGGTTT TACTCCTGAC GGTAGCCCTC CAAAGAGAGA TTCCGAGTAT 
TTTCGAGGTC GCTCACGAAA GTTTCAAATA CAAGCTGAAG GCCGATTCAA AAAGGAATAC 
AATGGAGATC AAGTCATTTA TGGCACACAG TTCGACCATA TGATCTCTAC CTTCCCAGAA 
AGTGCTTTCC GAGCTGGTAT GCGGATTGCC AAATATATTG ACCCTGCTGT GTATTATGAT 
AAATATGCCA GATCCCCGTA TATCATGTCA CCATTCGTCG CTTGTGTGAA TACACTCTCT 
GCATGGCCGG CTCCATCGCG TCTTGAAGAT GCTGTTATTT CATTAGTGGA AGCTGATTCT 
CAGGAATCAG ACACTGAATC GTTACCTGAA ATTAACGACT CCTCGGATGT CAGTTTATCA 
GACCTTCCCT CTACCAACGT TACTCCCAAG AAAACCACGA CACAAATAGA TGTTCAAACA 
AATATTGTTA CTCCGCCAAT TACTGTTACA GCAGTACCCG ACTCTCCAAA TCCCCCTGCT 
GCTACCCCCG CTACCGTTGC AGATAATGAT GATCTTAGTA TAGTATCCTC TGATTCTGGA 
AATACCACTG CACCTCGAAA AAATAGACAC CGGTATTGGA GTTTTGCTGG ATTTAGCGAT 
AGCACACGGT TTTCACATTT AAACGTTCCG GCTGCAATAC CGGATGCTCC ATCAGTTAAG 
ACTGAAACTT CCCAAAACGT GTCTCAATTA TCTGTAAAAT CTGCAGCTAC TTCTCTAGCT 
CCTGTGGAGG ATGATGAAGA CGATGAGCTT TTATTACATC GTCACATTAC CAATAAACAT 
AAGGATTTTA ATTTACATGT CAAGCAGCTA GGATTGCTTC ACAAACCTAG TTTGGACCTG 
GAAGAGGGTT ATATAATGGA TGAGAATTAT GGAAAGGACG TGTACAAGCA TGGTATTTCC 
AATGTTCCTG ATGACGAAAA TGAAGATGAC TTGCAACGTT ACTTTACTGC TTTAGAAATG 
CAACAGGATC AAAAGGAACA GCATAAGAAA TCTAAAAACA AAGACCCGTT TCGTAAAATT 
ACACATCCTT CTTTACATCT TGGAAAATTT TCCACGCCCA AACTAATAAA ACGTATGTCC 
CTTCGCTCAA AAAAAAGTTT AAGAAACGAT TCGAAATCGG ATGACGTCGG TAATTCTACT 
CATCGATTTT CTACCGCTAG TGCAGCATCC ACGTCTGCTG TAAAAACTGA AAAAGAAAAG 
AAAATGTCGG CGCCACGTCG CTCTCTTGAT AAATTAATTC GCATAGGTTC TTTACACCGT 
CACCATCATC ATCATCACAA AACCGACCTA ATTGAATCTG ACTCTGGAAT TGAAGCCAGT 
GAAAGTAACC GTCGTAAATC TGACATTTTT TCTTTTTCTG GAAGAAACTC ATTTTCCGTT 
TCTCGTCCTT CCTCTTCTCA TTCCACGTTG TCATATGCCA ATGATTCAGC ATCATCTGCT 
GTTAACGTGG CTGGAGAGAC TGGGTCGTTA CCTCCGCTAC GTGAACAAAC ATCTATAACC 
AGCGGTGTCC CACCTTCTAA TAGGCTTAAA AAACACGTTT CAACACCTGA AAAGATTGTT 
GAGGAGCGAT CGATTGATGA AGTATCACAA TCTAATACTC CTTCTTCAAA ACAATTGCCT 
CAATCTGTTG ACGGAAAGAC TGTTACCGCG AATGCTAATA GTACCACCGT CGCAAAGCAA 
CCCACGTCTA ATGTGGCGTT GACGAGGCCA AAGCCCGTGC GGACGGCTAC TAGTCAATCA 
AAAATACCGA AACCTGTTAA ACACATCCCT TCTGATTCAA ATAATCTCGA TCCACAACTT 
GGACCTTGGA GATTCGCCAA TCCCAAAGTG GATCCTATAG AAGACAATTC GTTTATTTTT 
GGTGAACACA AGTCAGTAAA GGAAAGACGA AAGTACTTTT CAAGCTCCAA ATTTTGTCGA 
GAAAATTTCT TTTACGATAA AGATGTAGTT TATTGCATGA GTTTCTTTTC CCCTCATATG 
GATTTTAATA CCTTTAATTT GAATATCGGA CCCATTCGGC TCAATGTTTA TAAGCATTTG 
AACTCTGATG GTCATCAGCC AATCCGATAT ATGATGCGTG AAACCGATGA TGAAGATGCT 
GTTATGTTTG TTGTCGAGTT TGACCTCCTC GAAGATGATG ATGAGACAGT TCTTGCCGAG 
CAGGCTAAGG AGCGTGAAAG AAGAAAACAA ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPCC594.01 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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