EFT201 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene
Clones
gRNAs

Summary

Gene Symbol	EFT201
Entrez Gene ID	3361483
Full Name	translation elongation factor 2 (EF-2) Eft2,A
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe 972h-

mRNA and Protein(s)

mRNA	Protein	Name
NM_001356092.1	NP_001342896.1	translation elongation factor 2 eft201
XM_001713021.2	XP_001713073.1	translation elongation factor 2 (EF-2) Eft2,A

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	eft201	XP_001713073.1
Magnaporthe oryzae (rice blast fungus)	MGG_01742	XP_003714739.1
Neurospora crassa	NCU07700	XP_962286.2
Danio rerio (zebrafish)	eef2l2	XP_697966.5
Saccharomyces cerevisiae (baker's yeast)	EFT2	NP_010673.1
Saccharomyces cerevisiae (baker's yeast)	EFT1	NP_014776.1
Arabidopsis thaliana (thale cress)	AT3G12915	NP_001154611.1
Oryza sativa (rice)	Os04g0118400	NP_001052057.1
Eremothecium gossypii	AGOS_AFR142C	NP_985689.1
Schizosaccharomyces pombe (fission yeast)	eft202	NP_587863.1
Xenopus tropicalis (tropical clawed frog)	eef2.2	NP_001015785.1
Kluyveromyces lactis	KLLA0E02993g	XP_454080.1
Oryza sativa (rice)	Os02g0519900	NP_001046972.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EFT201 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFT201 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj04921	XM_001713021.2 Latest version!	Schizosaccharomyces pombe 972h- translation elongation factor 2 (EF-2) Eft2,A (eft201), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OSc101934	NM_001356092.1 Latest version!	Schizosaccharomyces pombe translation elongation factor 2 eft201 (eft201), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFT201 ( XM_001713021.2 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> XP_001713073.1 Schizosaccharomyces pombe 972h- translation elongation factor 2 (EF-2) Eft2,A (eft201), mRNA.

Price & Availability↑

CloneID	OSj04921
Clone ID Related Accession (Same CDS sequence)	XM_001713021.2
Accession Version	XM_001713021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	translation elongation factor 2 (EF-2) Eft2,A
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001713021.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGGTTGCGT TCACTCCTGA AGAGGTCCGT AATTTGATGG GAAAACCATC CAATGTCCGT 
AACATGTCTG TGATTGCTCA CGTCGATCAC GGCAAGTCTA CGTTGACTGA TTCTTTGGTT 
CAAAAGGCCG GTATTATTTC TGCTGCCAAG GCTGGTGATG CTCGTTTCAT GGATACTCGT 
GCTGACGAAC AAGAGCGTGG TGTTACCATC AAGTCTACTG CCATTTCCTT GTTTGCTGAA 
ATGACTGACG ATGATATGAA GGATATGAAG GAGCCTGCTG ATGGTACTGA CTTCTTGGTT 
AACTTGATTG ACTCTCCCGG TCACGTTGAC TTTTCTTCTG AGGTTACTGC CGCTCTTCGT 
GTCACTGACG GTGCTTTGGT CGTTGTTGAT ACCATTGAGG GTGTCTGTGT CCAAACCGAG 
ACTGTGCTCC GTCAAGCTTT GGGTGAACGT ATTAGACCAG TTGTTGTTGT TAACAAAGTT 
GACCGTGCTT TGCTCGAACT TCAAATTTCT CAAGAAGAGC TTTATCAAAA TTTTGCTCGT 
GTCGTTGAAT CTGTTAACGT CGTTATTTCT ACTTATTACG ACAAGGTCCT TGGCGACTGC 
CAAGTTTTCC CTGACAAGGG TACTGTAGCT TTTGCTTCCG GTCTTCATGG TTGGGCTTTC 
ACTGTCCGTC AATTTGCCAA CCGTTATGCT AAGAAGTTTG GTATTGACCG TAACAAGATG 
ATGCAACGTC TTTGGGGTGA AAACTACTTC AACCCTAAGA CCAAGAAATG GTCCAAGTCT 
GCTACCGATG CTAACGGTAA CTCTAACCAA CGTGCTTTCA ACATGTTCAT CTTGGATCCC 
ATTTATCGTA TTTTCGATGC TGTTATGAAC AGCCGCAAAG ACGAGGTCTT CACTCTTTTG 
AGTAAGCTCG AGGTTACCAT CAAGCCCGAT GAGAAGGAGC TTGAGGGTAA GGCTCTCTTG 
AAGGTCGTTA TGCGCAAGTT CTTGCCTGCT GCTGATGCCC TTATGGAAAT GATTGTCCTT 
CATCTTCCTT CTCCTAAGAC TGCTCAACAA TATCGTGCTG AGACTTTGTA TGAAGGTCCC 
ATGGACGACG AGTGTGCTGT TGGTATCCGT AACTGTGATG CCAATGCTCC TCTTATGATC 
TACGTTTCTA AGATGGTTCC CACATCTGAT CGTGGTCGTT TCTATGCTTT TGGTCGTGTC 
TTCTCCGGTA CTGTCCGTTC TGGTTTGAAG GTCCGCATTC AAGGCCCTAA CTATGTTCCC 
GGTAAGAAGG ACGATCTCTT CATCAAGGCT ATCCAACGTA CCGTCCTTAT GATGGGTTCC 
AGAATTGAGC CTATTGAGGA TTGTCCTGCT GGTAACATTA TTGGTTTGGT CGGTGTTGAT 
CAATTTTTGG TCAAATCTGG TACTTTGACC ACTTCCGAAG TTGCCCACAA CATGAAGGTT 
ATGAAGTTTT CCGTCTCCCC AGTCGTCCAA GTCGCTGTTG AGGTTAAGAA CGGTAATGAT 
TTGCCTAAAC TTGTTGAAGG TCTTAAGCGT CTTTCCAAAT CCGACCCTTG TGTTTTGTGT 
ACCACCAGTG AATCTGGTGA ACACATTGTT GCTGGTGCTG GTGAGCTCCA CTTGGAGATT 
TGCTTGAAGG ATTTGCAAGA AGACCATGCT GGTATCCCTC TTAAGATTTC TCCTCCCGTC 
GTTTCTTACC GTGAGTCCGT TAGCGAGCCC TCTAGCATGA CTGCTCTCTC TAAGTCTCCC 
AACAAGCACA ACCGTATCTT CATGACTGCT GAGCCCATGA GTGAAGAACT CTCTGTCGCC 
ATTGAGACTG GTCACGTCAA TCCTCGTGAT GATTTCAAGG TTCGTGCTCG TATCATGGCT 
GACGAGTTTG GATGGGATGT CACTGATGCT CGTAAGATTT GGTGTTTCGG TCCTGACACC 
ACTGGTGCCA ATGTTGTTGT TGATCAAACC AAGGCTGTTG CTTACTTAAA CGAAATCAAG 
GATTCCGTTG TTGCTGCTTT TGCCTGGGCT TCCAAGGAGG GTCCCATGTT TGAGGAGAAC 
TTGCGTTCTT GCCGTTTCAA CATTCTTGAT GTTGTTTTGC ACGCTGATGC TATTCACCGT 
GGTGGTGGTC AAATTATTCC TACTGCTCGT CGTGTCGTCT ACGCTTCTAC CCTTTTGGCT 
TCTCCCATCA TCCAGGAGCC CGTTTTCTTG GTCGAAATCC AAGTTTCCGA AAACGCTATG 
GGTGGTATTT ACTCTGTCCT TAACAAGAAG CGTGGTCATG TCTTTTCTGA GGAGCAACGT 
GTTGGTACTC CCTTGTACAA CATCAAGGCT TACCTTCCCG TCAACGAGTC TTTCGGTTTC 
ACTGGTGAAC TTCGTCAAGC TACTGCTGGT CAAGCTTTCC CTCAATTGGT ATTTGACCAT 
TGGTCTCCCA TGTCTGGTGA TCCATTGGAT CCTACTTCTA AACCTGGTCA AATTGTTTGC 
GAAGCTCGTA AGCGTAAGGG TCTTAAGGAA AATGTTCCCG ACTACACTGA ATATTATGAC 
CGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713073.1
CDS	46..2574
Translation	MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDKVLGDCQVFPDKGTVAFASGLHGWAFTVRQFANRYAKKFGIDRNKMMQRLWGENYFNPKTKKWSKSATDANGNSNQRAFNMFILDPIYRIFDAVMNSRKDEVFTLLSKLEVTIKPDEKELEGKALLKVVMRKFLPAADALMEMIVLHLPSPKTAQQYRAETLYEGPMDDECAVGIRNCDANAPLMIYVSKMVPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVPDYTEYYDRL

Target ORF information:

RefSeq Version	XM_001713021.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- translation elongation factor 2 (EF-2) Eft2,A (eft201), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713021.2

ORF Insert Sequence:

ATGGTTGCGT TCACTCCTGA AGAGGTCCGT AATTTGATGG GAAAACCATC CAATGTCCGT 
AACATGTCTG TGATTGCTCA CGTCGATCAC GGCAAGTCTA CGTTGACTGA TTCTTTGGTT 
CAAAAGGCCG GTATTATTTC TGCTGCCAAG GCTGGTGATG CTCGTTTCAT GGATACTCGT 
GCTGACGAAC AAGAGCGTGG TGTTACCATC AAGTCTACTG CCATTTCCTT GTTTGCTGAA 
ATGACTGACG ATGATATGAA GGATATGAAG GAGCCTGCTG ATGGTACTGA CTTCTTGGTT 
AACTTGATTG ACTCTCCCGG TCACGTTGAC TTTTCTTCTG AGGTTACTGC CGCTCTTCGT 
GTCACTGACG GTGCTTTGGT CGTTGTTGAT ACCATTGAGG GTGTCTGTGT CCAAACCGAG 
ACTGTGCTCC GTCAAGCTTT GGGTGAACGT ATTAGACCAG TTGTTGTTGT TAACAAAGTT 
GACCGTGCTT TGCTCGAACT TCAAATTTCT CAAGAAGAGC TTTATCAAAA TTTTGCTCGT 
GTCGTTGAAT CTGTTAACGT CGTTATTTCT ACTTATTACG ACAAGGTCCT TGGCGACTGC 
CAAGTTTTCC CTGACAAGGG TACTGTAGCT TTTGCTTCCG GTCTTCATGG TTGGGCTTTC 
ACTGTCCGTC AATTTGCCAA CCGTTATGCT AAGAAGTTTG GTATTGACCG TAACAAGATG 
ATGCAACGTC TTTGGGGTGA AAACTACTTC AACCCTAAGA CCAAGAAATG GTCCAAGTCT 
GCTACCGATG CTAACGGTAA CTCTAACCAA CGTGCTTTCA ACATGTTCAT CTTGGATCCC 
ATTTATCGTA TTTTCGATGC TGTTATGAAC AGCCGCAAAG ACGAGGTCTT CACTCTTTTG 
AGTAAGCTCG AGGTTACCAT CAAGCCCGAT GAGAAGGAGC TTGAGGGTAA GGCTCTCTTG 
AAGGTCGTTA TGCGCAAGTT CTTGCCTGCT GCTGATGCCC TTATGGAAAT GATTGTCCTT 
CATCTTCCTT CTCCTAAGAC TGCTCAACAA TATCGTGCTG AGACTTTGTA TGAAGGTCCC 
ATGGACGACG AGTGTGCTGT TGGTATCCGT AACTGTGATG CCAATGCTCC TCTTATGATC 
TACGTTTCTA AGATGGTTCC CACATCTGAT CGTGGTCGTT TCTATGCTTT TGGTCGTGTC 
TTCTCCGGTA CTGTCCGTTC TGGTTTGAAG GTCCGCATTC AAGGCCCTAA CTATGTTCCC 
GGTAAGAAGG ACGATCTCTT CATCAAGGCT ATCCAACGTA CCGTCCTTAT GATGGGTTCC 
AGAATTGAGC CTATTGAGGA TTGTCCTGCT GGTAACATTA TTGGTTTGGT CGGTGTTGAT 
CAATTTTTGG TCAAATCTGG TACTTTGACC ACTTCCGAAG TTGCCCACAA CATGAAGGTT 
ATGAAGTTTT CCGTCTCCCC AGTCGTCCAA GTCGCTGTTG AGGTTAAGAA CGGTAATGAT 
TTGCCTAAAC TTGTTGAAGG TCTTAAGCGT CTTTCCAAAT CCGACCCTTG TGTTTTGTGT 
ACCACCAGTG AATCTGGTGA ACACATTGTT GCTGGTGCTG GTGAGCTCCA CTTGGAGATT 
TGCTTGAAGG ATTTGCAAGA AGACCATGCT GGTATCCCTC TTAAGATTTC TCCTCCCGTC 
GTTTCTTACC GTGAGTCCGT TAGCGAGCCC TCTAGCATGA CTGCTCTCTC TAAGTCTCCC 
AACAAGCACA ACCGTATCTT CATGACTGCT GAGCCCATGA GTGAAGAACT CTCTGTCGCC 
ATTGAGACTG GTCACGTCAA TCCTCGTGAT GATTTCAAGG TTCGTGCTCG TATCATGGCT 
GACGAGTTTG GATGGGATGT CACTGATGCT CGTAAGATTT GGTGTTTCGG TCCTGACACC 
ACTGGTGCCA ATGTTGTTGT TGATCAAACC AAGGCTGTTG CTTACTTAAA CGAAATCAAG 
GATTCCGTTG TTGCTGCTTT TGCCTGGGCT TCCAAGGAGG GTCCCATGTT TGAGGAGAAC 
TTGCGTTCTT GCCGTTTCAA CATTCTTGAT GTTGTTTTGC ACGCTGATGC TATTCACCGT 
GGTGGTGGTC AAATTATTCC TACTGCTCGT CGTGTCGTCT ACGCTTCTAC CCTTTTGGCT 
TCTCCCATCA TCCAGGAGCC CGTTTTCTTG GTCGAAATCC AAGTTTCCGA AAACGCTATG 
GGTGGTATTT ACTCTGTCCT TAACAAGAAG CGTGGTCATG TCTTTTCTGA GGAGCAACGT 
GTTGGTACTC CCTTGTACAA CATCAAGGCT TACCTTCCCG TCAACGAGTC TTTCGGTTTC 
ACTGGTGAAC TTCGTCAAGC TACTGCTGGT CAAGCTTTCC CTCAATTGGT ATTTGACCAT 
TGGTCTCCCA TGTCTGGTGA TCCATTGGAT CCTACTTCTA AACCTGGTCA AATTGTTTGC 
GAAGCTCGTA AGCGTAAGGG TCTTAAGGAA AATGTTCCCG ACTACACTGA ATATTATGAC 
CGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFT201 ( NM_001356092.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001342896.1 Schizosaccharomyces pombe translation elongation factor 2 eft201 (eft201), mRNA.

Price & Availability↑

CloneID	OSc101934
Clone ID Related Accession (Same CDS sequence)	NM_001356092.1
Accession Version	NM_001356092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	translation elongation factor 2 eft201
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGGTTGCGT TCACTCCTGA AGAGGTCCGT AATTTGATGG GAAAACCATC CAATGTCCGT 
AACATGTCTG TGATTGCTCA CGTCGATCAC GGCAAGTCTA CGTTGACTGA TTCTTTGGTT 
CAAAAGGCCG GTATTATTTC TGCTGCCAAG GCTGGTGATG CTCGTTTCAT GGATACTCGT 
GCTGACGAAC AAGAGCGTGG TGTTACCATC AAGTCTACTG CCATTTCCTT GTTTGCTGAA 
ATGACTGACG ATGATATGAA GGATATGAAG GAGCCTGCTG ATGGTACTGA CTTCTTGGTT 
AACTTGATTG ACTCTCCCGG TCACGTTGAC TTTTCTTCTG AGGTTACTGC CGCTCTTCGT 
GTCACTGACG GTGCTTTGGT CGTTGTTGAT ACCATTGAGG GTGTCTGTGT CCAAACCGAG 
ACTGTGCTCC GTCAAGCTTT GGGTGAACGT ATTAGACCAG TTGTTGTTGT TAACAAAGTT 
GACCGTGCTT TGCTCGAACT TCAAATTTCT CAAGAAGAGC TTTATCAAAA TTTTGCTCGT 
GTCGTTGAAT CTGTTAACGT CGTTATTTCT ACTTATTACG ACAAGGTCCT TGGCGACTGC 
CAAGTTTTCC CTGACAAGGG TACTGTAGCT TTTGCTTCCG GTCTTCATGG TTGGGCTTTC 
ACTGTCCGTC AATTTGCCAA CCGTTATGCT AAGAAGTTTG GTATTGACCG TAACAAGATG 
ATGCAACGTC TTTGGGGTGA AAACTACTTC AACCCTAAGA CCAAGAAATG GTCCAAGTCT 
GCTACCGATG CTAACGGTAA CTCTAACCAA CGTGCTTTCA ACATGTTCAT CTTGGATCCC 
ATTTATCGTA TTTTCGATGC TGTTATGAAC AGCCGCAAAG ACGAGGTCTT CACTCTTTTG 
AGTAAGCTCG AGGTTACCAT CAAGCCCGAT GAGAAGGAGC TTGAGGGTAA GGCTCTCTTG 
AAGGTCGTTA TGCGCAAGTT CTTGCCTGCT GCTGATGCCC TTATGGAAAT GATTGTCCTT 
CATCTTCCTT CTCCTAAGAC TGCTCAACAA TATCGTGCTG AGACTTTGTA TGAAGGTCCC 
ATGGACGACG AGTGTGCTGT TGGTATCCGT AACTGTGATG CCAATGCTCC TCTTATGATC 
TACGTTTCTA AGATGGTTCC CACATCTGAT CGTGGTCGTT TCTATGCTTT TGGTCGTGTC 
TTCTCCGGTA CTGTCCGTTC TGGTTTGAAG GTCCGCATTC AAGGCCCTAA CTATGTTCCC 
GGTAAGAAGG ACGATCTCTT CATCAAGGCT ATCCAACGTA CCGTCCTTAT GATGGGTTCC 
AGAATTGAGC CTATTGAGGA TTGTCCTGCT GGTAACATTA TTGGTTTGGT CGGTGTTGAT 
CAATTTTTGG TCAAATCTGG TACTTTGACC ACTTCCGAAG TTGCCCACAA CATGAAGGTT 
ATGAAGTTTT CCGTCTCCCC AGTCGTCCAA GTCGCTGTTG AGGTTAAGAA CGGTAATGAT 
TTGCCTAAAC TTGTTGAAGG TCTTAAGCGT CTTTCCAAAT CCGACCCTTG TGTTTTGTGT 
ACCACCAGTG AATCTGGTGA ACACATTGTT GCTGGTGCTG GTGAGCTCCA CTTGGAGATT 
TGCTTGAAGG ATTTGCAAGA AGACCATGCT GGTATCCCTC TTAAGATTTC TCCTCCCGTC 
GTTTCTTACC GTGAGTCCGT TAGCGAGCCC TCTAGCATGA CTGCTCTCTC TAAGTCTCCC 
AACAAGCACA ACCGTATCTT CATGACTGCT GAGCCCATGA GTGAAGAACT CTCTGTCGCC 
ATTGAGACTG GTCACGTCAA TCCTCGTGAT GATTTCAAGG TTCGTGCTCG TATCATGGCT 
GACGAGTTTG GATGGGATGT CACTGATGCT CGTAAGATTT GGTGTTTCGG TCCTGACACC 
ACTGGTGCCA ATGTTGTTGT TGATCAAACC AAGGCTGTTG CTTACTTAAA CGAAATCAAG 
GATTCCGTTG TTGCTGCTTT TGCCTGGGCT TCCAAGGAGG GTCCCATGTT TGAGGAGAAC 
TTGCGTTCTT GCCGTTTCAA CATTCTTGAT GTTGTTTTGC ACGCTGATGC TATTCACCGT 
GGTGGTGGTC AAATTATTCC TACTGCTCGT CGTGTCGTCT ACGCTTCTAC CCTTTTGGCT 
TCTCCCATCA TCCAGGAGCC CGTTTTCTTG GTCGAAATCC AAGTTTCCGA AAACGCTATG 
GGTGGTATTT ACTCTGTCCT TAACAAGAAG CGTGGTCATG TCTTTTCTGA GGAGCAACGT 
GTTGGTACTC CCTTGTACAA CATCAAGGCT TACCTTCCCG TCAACGAGTC TTTCGGTTTC 
ACTGGTGAAC TTCGTCAAGC TACTGCTGGT CAAGCTTTCC CTCAATTGGT ATTTGACCAT 
TGGTCTCCCA TGTCTGGTGA TCCATTGGAT CCTACTTCTA AACCTGGTCA AATTGTTTGC 
GAAGCTCGTA AGCGTAAGGG TCTTAAGGAA AATGTTCCCG ACTACACTGA ATATTATGAC 
CGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342896.1
CDS	46..2574
Translation	MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDKVLGDCQVFPDKGTVAFASGLHGWAFTVRQFANRYAKKFGIDRNKMMQRLWGENYFNPKTKKWSKSATDANGNSNQRAFNMFILDPIYRIFDAVMNSRKDEVFTLLSKLEVTIKPDEKELEGKALLKVVMRKFLPAADALMEMIVLHLPSPKTAQQYRAETLYEGPMDDECAVGIRNCDANAPLMIYVSKMVPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVPDYTEYYDRL

Target ORF information:

RefSeq Version	NM_001356092.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe translation elongation factor 2 eft201 (eft201), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356092.1

ORF Insert Sequence:

ATGGTTGCGT TCACTCCTGA AGAGGTCCGT AATTTGATGG GAAAACCATC CAATGTCCGT 
AACATGTCTG TGATTGCTCA CGTCGATCAC GGCAAGTCTA CGTTGACTGA TTCTTTGGTT 
CAAAAGGCCG GTATTATTTC TGCTGCCAAG GCTGGTGATG CTCGTTTCAT GGATACTCGT 
GCTGACGAAC AAGAGCGTGG TGTTACCATC AAGTCTACTG CCATTTCCTT GTTTGCTGAA 
ATGACTGACG ATGATATGAA GGATATGAAG GAGCCTGCTG ATGGTACTGA CTTCTTGGTT 
AACTTGATTG ACTCTCCCGG TCACGTTGAC TTTTCTTCTG AGGTTACTGC CGCTCTTCGT 
GTCACTGACG GTGCTTTGGT CGTTGTTGAT ACCATTGAGG GTGTCTGTGT CCAAACCGAG 
ACTGTGCTCC GTCAAGCTTT GGGTGAACGT ATTAGACCAG TTGTTGTTGT TAACAAAGTT 
GACCGTGCTT TGCTCGAACT TCAAATTTCT CAAGAAGAGC TTTATCAAAA TTTTGCTCGT 
GTCGTTGAAT CTGTTAACGT CGTTATTTCT ACTTATTACG ACAAGGTCCT TGGCGACTGC 
CAAGTTTTCC CTGACAAGGG TACTGTAGCT TTTGCTTCCG GTCTTCATGG TTGGGCTTTC 
ACTGTCCGTC AATTTGCCAA CCGTTATGCT AAGAAGTTTG GTATTGACCG TAACAAGATG 
ATGCAACGTC TTTGGGGTGA AAACTACTTC AACCCTAAGA CCAAGAAATG GTCCAAGTCT 
GCTACCGATG CTAACGGTAA CTCTAACCAA CGTGCTTTCA ACATGTTCAT CTTGGATCCC 
ATTTATCGTA TTTTCGATGC TGTTATGAAC AGCCGCAAAG ACGAGGTCTT CACTCTTTTG 
AGTAAGCTCG AGGTTACCAT CAAGCCCGAT GAGAAGGAGC TTGAGGGTAA GGCTCTCTTG 
AAGGTCGTTA TGCGCAAGTT CTTGCCTGCT GCTGATGCCC TTATGGAAAT GATTGTCCTT 
CATCTTCCTT CTCCTAAGAC TGCTCAACAA TATCGTGCTG AGACTTTGTA TGAAGGTCCC 
ATGGACGACG AGTGTGCTGT TGGTATCCGT AACTGTGATG CCAATGCTCC TCTTATGATC 
TACGTTTCTA AGATGGTTCC CACATCTGAT CGTGGTCGTT TCTATGCTTT TGGTCGTGTC 
TTCTCCGGTA CTGTCCGTTC TGGTTTGAAG GTCCGCATTC AAGGCCCTAA CTATGTTCCC 
GGTAAGAAGG ACGATCTCTT CATCAAGGCT ATCCAACGTA CCGTCCTTAT GATGGGTTCC 
AGAATTGAGC CTATTGAGGA TTGTCCTGCT GGTAACATTA TTGGTTTGGT CGGTGTTGAT 
CAATTTTTGG TCAAATCTGG TACTTTGACC ACTTCCGAAG TTGCCCACAA CATGAAGGTT 
ATGAAGTTTT CCGTCTCCCC AGTCGTCCAA GTCGCTGTTG AGGTTAAGAA CGGTAATGAT 
TTGCCTAAAC TTGTTGAAGG TCTTAAGCGT CTTTCCAAAT CCGACCCTTG TGTTTTGTGT 
ACCACCAGTG AATCTGGTGA ACACATTGTT GCTGGTGCTG GTGAGCTCCA CTTGGAGATT 
TGCTTGAAGG ATTTGCAAGA AGACCATGCT GGTATCCCTC TTAAGATTTC TCCTCCCGTC 
GTTTCTTACC GTGAGTCCGT TAGCGAGCCC TCTAGCATGA CTGCTCTCTC TAAGTCTCCC 
AACAAGCACA ACCGTATCTT CATGACTGCT GAGCCCATGA GTGAAGAACT CTCTGTCGCC 
ATTGAGACTG GTCACGTCAA TCCTCGTGAT GATTTCAAGG TTCGTGCTCG TATCATGGCT 
GACGAGTTTG GATGGGATGT CACTGATGCT CGTAAGATTT GGTGTTTCGG TCCTGACACC 
ACTGGTGCCA ATGTTGTTGT TGATCAAACC AAGGCTGTTG CTTACTTAAA CGAAATCAAG 
GATTCCGTTG TTGCTGCTTT TGCCTGGGCT TCCAAGGAGG GTCCCATGTT TGAGGAGAAC 
TTGCGTTCTT GCCGTTTCAA CATTCTTGAT GTTGTTTTGC ACGCTGATGC TATTCACCGT 
GGTGGTGGTC AAATTATTCC TACTGCTCGT CGTGTCGTCT ACGCTTCTAC CCTTTTGGCT 
TCTCCCATCA TCCAGGAGCC CGTTTTCTTG GTCGAAATCC AAGTTTCCGA AAACGCTATG 
GGTGGTATTT ACTCTGTCCT TAACAAGAAG CGTGGTCATG TCTTTTCTGA GGAGCAACGT 
GTTGGTACTC CCTTGTACAA CATCAAGGCT TACCTTCCCG TCAACGAGTC TTTCGGTTTC 
ACTGGTGAAC TTCGTCAAGC TACTGCTGGT CAAGCTTTCC CTCAATTGGT ATTTGACCAT 
TGGTCTCCCA TGTCTGGTGA TCCATTGGAT CCTACTTCTA AACCTGGTCA AATTGTTTGC 
GAAGCTCGTA AGCGTAAGGG TCTTAAGGAA AATGTTCCCG ACTACACTGA ATATTATGAC 
CGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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EFT201 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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