SIP1 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene
Clones
gRNAs

Summary

Gene Symbol	SIP1
Entrez Gene ID	2540316
Full Name	Pof6 interacting protein Sip1, predicted AP-1 accessory protein
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe 972h-

mRNA and Protein(s)

mRNA	Protein	Name
NM_001356216.1	NP_001342706.1	Pof6-interacting protein Sip1 putative AP-1 accessory protein
NM_001021451.2	NP_595540.1	Pof6 interacting protein Sip1, predicted AP-1 accessory protein

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04982	XP_003712481.1
Schizosaccharomyces pombe (fission yeast)	sip1	NP_595540.1
Neurospora crassa	NCU08993	XP_959021.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sip1 recruits Rho3 to the Golgi/endosomes through physical interaction and enhances the formation of the Golgi/endosome AP-1/Rho3 complex, thereby promoting crosstalk between AP-1 and Rho3 in the regulation of Golgi/endosomal trafficking in fission yeast.
Title: Sip1, an AP-1 accessory protein in fission yeast, is required for localization of Rho3 GTPase.

Findings reveal a role for Sip1 in the regulation of Golgi/endosome trafficking in coordination with the AP-1 complex, and identified Bgs1, required for cell wall synthesis, as the new cargo of AP-1-dependent trafficking.
Title: Sip1, a conserved AP-1 accessory protein, is important for Golgi/endosome trafficking in fission yeast.

Study identified a novel conserved protein, Sip1, as an interacting partner of an essential F-box protein Pof6.
Title: Identification of a conserved F-box protein 6 interactor essential for endocytosis and cytokinesis in fission yeast.

The following SIP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100350	NM_001356216.1 Latest version!	Schizosaccharomyces pombe Pof6-interacting protein Sip1 putative AP-1 accessory protein (sip1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSj01612	NM_001021451.2 Latest version!	Schizosaccharomyces pombe 972h- Pof6 interacting protein Sip1, predicted AP-1 accessory protein (sip1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIP1 ( NM_001356216.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001342706.1 Schizosaccharomyces pombe Pof6-interacting protein Sip1 putative AP-1 accessory protein (sip1), mRNA.

Price & Availability↑

CloneID	OSc100350
Clone ID Related Accession (Same CDS sequence)	NM_001356216.1
Accession Version	NM_001356216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Pof6-interacting protein Sip1 putative AP-1 accessory protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

ATGTCGTTAG CATCATTGCC GCTCGAAAAG TTGACGAAAG TAGCTGATGT AGATAAGAGG 
GAGACCATAT ATATTGATTT ACTCAATTAC GAAAAACAAT TGAGGAATCT GAACAAAAAT 
ACTCGCGAGG ATTCATTACA AACTAATTTA AATCCCCTAC TTTCGTTAAT CAAAAAATAT 
GGAAATTTTA TTGCTTATCC AACTAGAAAA CTTCTTTCTA ACTGTATAAT TGCACTCTTA 
AAAGGACTCG AGGGTTATCA TCAGCATCGA GTTTTACTTT ACTTCGTGGA TAGCATTCAT 
GAGAAAAAAG ACTATATAGA GATTATTAAC GTTTTATCCG TTCTGACTTC TTGCTTGGAA 
GAATATGGAA ATCAGATCAA TCAGCAAGAG ACTTTTATAA GATTTTTCTT CAAATATTCG 
AAAACCAATT TGTCATATGG AGTTCGCTCA ATCGCATTAG ATGGACTGTC GAAATGCATT 
GAAACATCTA TTCAGTCTAC GAATGAAGAT TTGATAAAAG AAATATGGAA ATGTATTAAA 
TCAAATTTAA ACACTTCATC AACGAATGTT CTTTTATCTG CACTTCGTTG CGCTTACTAC 
ATTCTGGGCT CTTCACGTCT TCGTAATTCA GAATTGGAAT GGTTCAAATC TTTTCTATTT 
AAAAAATCAT CAATTGATAA TTCTTTGCTC CATTCTTCTT TAGGAAGATG CTTTTCTGCA 
CTTGCAAGTG CTCAAGCTGA CAAATCGATT GAAGCCGCTG CAAACGCAGT ACCCTCAACT 
CCAGCGGATG ATGAGAACAA TGAGGAAGTA GAAAATCGGT CTACTACTCG CCGTTTTGAT 
GAAACTGAGA GTTTTCGTCA CGCCCTACGG ATTCTTGTCG AAAATTATCA CTCCAAGCAT 
GCAAAGTATG AAAGCTCAAA AGCGGTTCTT GTTTTTGCCG TTAAGCATCT TTTTCTAATG 
CAACGTCCAT CCTTTGTTTC TCTCAACTAC ACTACTATAG TGGATATTAT TCTATTACAC 
CAATCACACT CTACTTCAAA AGAATGGTTG TCTTCGTTGG AGCAATATCA TTGTACATAC 
TTGTTTCGCG ATGTGATTGG AAGGCGATTT CTCGATCAAA GCTCTGAATT GCAGGGAATT 
GAAATACTTT TAAAGAGAGT GTTAGAGCCT TATTTAAAAG ATCAGTCGAA GATTTCGGAA 
ATTGCTGTTA AGACTGCGAT GGCTTCCACC GCTGATATAT TAAGCCGATC AAAGGGTTCG 
GCTACTGCTT TATATAGTCA GTTACTAGAT ACTACACTAC AGATTCTATA CTTGGGTAAA 
GATTCATTGA AGAATGAAGT AGCCTGGTTG CTGCGCTGTT TACTATCCGT TATGCCTTCT 
CCTTTAATGC CCACTTTGCT TGCTCTTTTT AATAGATTAG AGTTGGAATT TAAGCACCTT 
CGTTCTAAAC CTAAGGAAGT AAGTCCCAAA TTGAATGTTG GTATTTTCAA TAATCTTACT 
TTGTCGTTGA TGTCGATTTA TGTTGCCGCC GAAGAAAATC CTCTATATGT TGCTTCTTCT 
ATATTTTCAA AGTTATTTAA CTTCTCTTTG GATTTGATTA AGTCATCATC TAAATTGGAT 
TTATTAGCGG CACAAGCACA AATCAAGACT GGTTGGCGAC TATTAAGCGC TTACGTTTCT 
TTTGGTCCTT CTTTTACTAG AATGCAATTG TCCCAATTGC TAATTTTATG GAAGAATGCC 
CTTTCCAGAG TACCTTCAAA AATACATTCA CAAAATTTCA TGGAGACAAA TGCAGACATG 
TTTAATCGGT TTGAAGCGTT ACAGTGCTTG CTTTCGTTTT TGGAGTATAA CAAGATTTTA 
TTCACACCTG ATGTTAGGAA GCGAGTCATT ATTTTCCTAA ATAACTGCCT TAAATTCTAC 
AAAGCTTTTT CGTTAACCAA AAGGCTTGAA ATTACCATGC CAATTCCCGC TTCAGATTAT 
TCTCACTTGG AACTTCGCTC TCTCTTGCTT GCTCGGATTT TCCAATGTTT CTTCGAGTTG 
TCTCAGTTAA GTGGAATGAG TTTAGATGAT CAAGAATTAC TATCCACGAC TGTGTCTACT 
ATAGCCGAAC TTTCTTCTTT ACAAGGATAT GATACTGCGG ACGAAATAGT TCAAGCGAAA 
GCCTTTACTT CTCCAAGAGT TATACCTGGA ATTGGTTCAC GATTTTACGC TCAGCCAGAG 
GAAGAATTTC AAACAACCAT GGATGATATA CTTCCTTGTT ATATTTGCGA GTCATCTTCT 
GATTCCCTGT CAGTGATTTC GAGATCAAAC TCTGCAAAAC TTCCTTACGT ACCCCCGTCT 
TTAACTTCTT TAATTGATAA CGCGATCCAG TTATTTGCGT CTATGTTTTG TCATCAACCT 
CCGAAGGTTA TGGAGAGCAC TCTCGAAATT ATGGTAGCGT CTGTGACAAA CCTCAAGAGT 
GGGAGAGATA TTTTACGTTA CAAGGCAGTA ACAGCAAATA CTTTATTTTC CATTTATGGA 
TTTCTCTCTG CTTTCGATAA CAAAAAAGTT ACCTTGCCTG ACAAGGTTGT ATCCCTTATG 
ATAGAATTGC TCGAAAACTT GACTGCTGTT AACGATCCGA AGTTAATTCC TTACATTTCG 
AAATCTTATG CGTTTTTGGC ATATTTATAT CAAAGCAAAC CGACGTCCGT TTTGGATACA 
ATAATTAAAC GTGCTGCAGA GTCGGGAGAT CCAAATTGCA GGGCTGTCTT GATTCTAGCC 
GTTGGCCATA TATTAAAAAA GGTTGGTTCC GGAGTTCCCC AAACTACCCT CATCACCGCT 
TATCAGCTGC TCAAAGTTTT TAATACTGAT TCGAATGACC TTGTTAGAGA GTGTTCTATT 
CGTGCTTCTT CCGTTTCTCT TGGGTCTATC GCAAAGACCA TTCCGAGTGT TTTAACTGCC 
CAGACACGCA TTTTATTGAG CTATATGCTA TTGCCAGAAT GTGATATTGA TGTTCAGCTC 
TTAAAGAAGA GAAACAGTTT ATTTTTCCCG TCCACTCTTT TTGCCAATTA CTTGGATTGT 
TTGATAAATG AGCTTGGACC AAACTTGCGA TCCGATTCTT CTACTTCGCA TTTCGCATTC 
GATTTAGCTC ACCAATTGCT TTTACTTAGT AGAGATACGG GAGATAACAT TATACACGTT 
TACAAATGTT ATCAGCACCT ATATCTATTT ACACCATTAG AAACCAATTC TGATTTCTTC 
ATTAGGGAGC TTTGTGGTAA CATAATTGAC CTAAAGCAAC CTAAACGTAG GGAAATTTCT 
ATTGAAGTTT TATACCAAAT ATTGCTTCAA AACCATGAGT TCTCGGAATC ATGTTATAAG 
CGACATTTTG ATAGGCTTAT TTGGCAATCA CTCGACACCA CTCCTGACAA CAAGCTTTTG 
AAGAACATAC TGTATATCTG GCTAAACGAT TACAAAGATT CGGACATACA CTCATGGGTA 
AATATTTTAC TTTTTCTTGT CGGCATAAAA AATACTTCGA GTTCTGAAAG ATCCAACGAA 
CAAGATATAC AAAACAACCT AGATGAAGAT ACCGTATCTT TGGCTTATGG AACTTCCGAA 
AACGCAAGTA ATCCATTATT CCAGAAGAAT TACCGTTGGC AGACGCAGTT TTATGCAGCT 
ACATGCCTTA AAACATGTAT CTCTGAAGTG ATGGCCGAAA ATAAAGAGCC CGCAAAATAC 
TTGATTTCTA GGATATCTGA CCTAGTCCGT GCTGCTTTTG TTTTGTCTAC TTCAGACAAC 
TTTTACTTGA AGCAGGGGGG TTTTGAACTT TTGGATACTA TCATAACGGA CTTTTCGAAT 
GTTATGGATC CTGACTTTGA CGATGTTTCT CTTTTGGACC AATTTCAAGC TCAAATCAGC 
TCTGCTTTTA GTTCCGCGTT TGTAGATGAT TCATCTCCTG AAATAGTAGC CATGTCTCTT 
AATATAGCTT CCAATTTTAT TGGAACTGGA CTTCTAAAAA CCGAATCGCA AGCAGCTAAA 
ATCCTCAATT TATTGAAAGA AGCGTTGGAA TCTAGTTTAC CAGAGAGGGC TGGATTTGAG 
GAAAATCGTC ACTTTAAGTA TGACTCGAAA TATTTTCTTC GTATTGCTAC TTTATCAGCT 
TGGGCTTCTT TGCTGGTACA TTCAGCGAAT GTGTCATATT TGAAAGAATT TCTTTCCTCT 
AACATCCGAA GGCTATCTTC GCATTGGATT ATGGCTTTAA GAGGATATTC CAGATTGCAA 
TTTGGTCCTA GTTTAGTAAA AGATTTTGTA GCTGATACCT CTTTCACTCG GGTCCAAAGT 
ATAAACCCTA AAATACTTCT CAATATTTAT GAGAAATCTT GGCTGAACAT CGCAGAGTCA 
TTAACAATAT TACTTTCTAC TGATGCAAAT CTGATTTATG GAATATTAAG TGGAGATGAG 
CTGTCGCTTT CTGAGGAGGA CGTATTCTTC CAAGACAACA TAAATTATGC TTCCAACAGG 
TACTCTTTTA ACTTTTTTTT GTTTAGTGTC TGCTTCCAAT CTCTCTTAAT TCCAAGCACT 
TTGCAAGGCT TTAGAAATCC AGTTTTCAGA ATTATGCGAA TTATGACTGA AGTTTTAACT 
GAAGAATTAT GTACCAAGGT TCTTTATGAC CATAATGTGT TCCCCGAAGT TGTTGATCTT 
TTAGTTCGTT TGATTTTGAC GGAGGATTGG GAAACGAAGG CTAGTATTGT CATTTTTGTG 
AAAAAGTTAG CTCTTGCTAA CCCGGCACAC AATGACTTGA AGTTTTGTGA AACAGATTCT 
GTAACATCTA TGGAGCCTAC TGTATCAGAA AGCGTCGAAA TGCTTTTTCA ATTGGTCAAA 
ATATTGACAT TAGCTTTAAC GGTTAGAGTG CCGGGCCTAC GTTCTGATCC AGCTGAATCA 
GCTACCAATA AAAAAGCTAG TGAATTTGTT ATTTTGTGTT TTAATGCATT TCTTGATGTT 
TCCAACATTT TCCCCGCTAT TGTGCGTGTT GATTTATTTG CGACAGCAAT GCATGTTTAT 
GAAGTCATTA TGGACGATCA ACAACTATTA AAAAATTCTA AGCAAGAGTT GTTAGCTGCT 
CTTAGAAAGT TGTTGAGTAC AATGGTTGAG CAAAATGATC ACACATGTAC AACTTTGATA 
TACACTCTTT TTGAACACCT CCTTAACATC TACAAAGAAA CGATTAATGA TAGTGATAAA 
AAGGAGAAAA ACGAAACTGC TTTTATGAGC ATGGTCCTTG TACTTACTCT TGCAGCTCCT 
ATATTAGACA GTGAGCAGCT TGTGGTACAC GATTTTTTGG AAGAACTATT CTCTTCCTTG 
AAGAGCTCAG AAGAGGACTT TGCTTTAAGA ACTCGTTGTG TAACATCGCT GATGTTAGTT 
AACAGTAAAC ACCCAAGCAT GAGCGCTCTT TGCCGTTTCA TTATTTACAA ATCGGTTACG 
ATGCTACAGA ACGGTGAAGT TCTGAAAAGT GGTGATTATG TCACGGAGTT TATACCTGCA 
TTGCTTGATA TGCATGTCCA TATTCCGGAG GAGAAGAAAA AATCATTTTT AAGCATGTCT 
ATTCCGATAG CTGCACATTT TAGTATCCTC GTCGAGCACG CTGACGCACG AAAAAGAATC 
GGTGAAAAAC TGCTGAAGAT TATGGCTATT CAGCCAGATG AGGCCAAAGC AATCATTCAA 
CAACTTTCTC CTAGGCAAAA AAAATGCGTT GAAGAGATCT TAATACAAAA TGTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342706.1
CDS	36..5795
Translation	MSLASLPLEKLTKVADVDKRETIYIDLLNYEKQLRNLNKNTREDSLQTNLNPLLSLIKKYGNFIAYPTRKLLSNCIIALLKGLEGYHQHRVLLYFVDSIHEKKDYIEIINVLSVLTSCLEEYGNQINQQETFIRFFFKYSKTNLSYGVRSIALDGLSKCIETSIQSTNEDLIKEIWKCIKSNLNTSSTNVLLSALRCAYYILGSSRLRNSELEWFKSFLFKKSSIDNSLLHSSLGRCFSALASAQADKSIEAAANAVPSTPADDENNEEVENRSTTRRFDETESFRHALRILVENYHSKHAKYESSKAVLVFAVKHLFLMQRPSFVSLNYTTIVDIILLHQSHSTSKEWLSSLEQYHCTYLFRDVIGRRFLDQSSELQGIEILLKRVLEPYLKDQSKISEIAVKTAMASTADILSRSKGSATALYSQLLDTTLQILYLGKDSLKNEVAWLLRCLLSVMPSPLMPTLLALFNRLELEFKHLRSKPKEVSPKLNVGIFNNLTLSLMSIYVAAEENPLYVASSIFSKLFNFSLDLIKSSSKLDLLAAQAQIKTGWRLLSAYVSFGPSFTRMQLSQLLILWKNALSRVPSKIHSQNFMETNADMFNRFEALQCLLSFLEYNKILFTPDVRKRVIIFLNNCLKFYKAFSLTKRLEITMPIPASDYSHLELRSLLLARIFQCFFELSQLSGMSLDDQELLSTTVSTIAELSSLQGYDTADEIVQAKAFTSPRVIPGIGSRFYAQPEEEFQTTMDDILPCYICESSSDSLSVISRSNSAKLPYVPPSLTSLIDNAIQLFASMFCHQPPKVMESTLEIMVASVTNLKSGRDILRYKAVTANTLFSIYGFLSAFDNKKVTLPDKVVSLMIELLENLTAVNDPKLIPYISKSYAFLAYLYQSKPTSVLDTIIKRAAESGDPNCRAVLILAVGHILKKVGSGVPQTTLITAYQLLKVFNTDSNDLVRECSIRASSVSLGSIAKTIPSVLTAQTRILLSYMLLPECDIDVQLLKKRNSLFFPSTLFANYLDCLINELGPNLRSDSSTSHFAFDLAHQLLLLSRDTGDNIIHVYKCYQHLYLFTPLETNSDFFIRELCGNIIDLKQPKRREISIEVLYQILLQNHEFSESCYKRHFDRLIWQSLDTTPDNKLLKNILYIWLNDYKDSDIHSWVNILLFLVGIKNTSSSERSNEQDIQNNLDEDTVSLAYGTSENASNPLFQKNYRWQTQFYAATCLKTCISEVMAENKEPAKYLISRISDLVRAAFVLSTSDNFYLKQGGFELLDTIITDFSNVMDPDFDDVSLLDQFQAQISSAFSSAFVDDSSPEIVAMSLNIASNFIGTGLLKTESQAAKILNLLKEALESSLPERAGFEENRHFKYDSKYFLRIATLSAWASLLVHSANVSYLKEFLSSNIRRLSSHWIMALRGYSRLQFGPSLVKDFVADTSFTRVQSINPKILLNIYEKSWLNIAESLTILLSTDANLIYGILSGDELSLSEEDVFFQDNINYASNRYSFNFFLFSVCFQSLLIPSTLQGFRNPVFRIMRIMTEVLTEELCTKVLYDHNVFPEVVDLLVRLILTEDWETKASIVIFVKKLALANPAHNDLKFCETDSVTSMEPTVSESVEMLFQLVKILTLALTVRVPGLRSDPAESATNKKASEFVILCFNAFLDVSNIFPAIVRVDLFATAMHVYEVIMDDQQLLKNSKQELLAALRKLLSTMVEQNDHTCTTLIYTLFEHLLNIYKETINDSDKKEKNETAFMSMVLVLTLAAPILDSEQLVVHDFLEELFSSLKSSEEDFALRTRCVTSLMLVNSKHPSMSALCRFIIYKSVTMLQNGEVLKSGDYVTEFIPALLDMHVHIPEEKKKSFLSMSIPIAAHFSILVEHADARKRIGEKLLKIMAIQPDEAKAIIQQLSPRQKKCVEEILIQNVE

Target ORF information:

RefSeq Version	NM_001356216.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Pof6-interacting protein Sip1 putative AP-1 accessory protein (sip1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356216.1

ORF Insert Sequence:

ATGTCGTTAG CATCATTGCC GCTCGAAAAG TTGACGAAAG TAGCTGATGT AGATAAGAGG 
GAGACCATAT ATATTGATTT ACTCAATTAC GAAAAACAAT TGAGGAATCT GAACAAAAAT 
ACTCGCGAGG ATTCATTACA AACTAATTTA AATCCCCTAC TTTCGTTAAT CAAAAAATAT 
GGAAATTTTA TTGCTTATCC AACTAGAAAA CTTCTTTCTA ACTGTATAAT TGCACTCTTA 
AAAGGACTCG AGGGTTATCA TCAGCATCGA GTTTTACTTT ACTTCGTGGA TAGCATTCAT 
GAGAAAAAAG ACTATATAGA GATTATTAAC GTTTTATCCG TTCTGACTTC TTGCTTGGAA 
GAATATGGAA ATCAGATCAA TCAGCAAGAG ACTTTTATAA GATTTTTCTT CAAATATTCG 
AAAACCAATT TGTCATATGG AGTTCGCTCA ATCGCATTAG ATGGACTGTC GAAATGCATT 
GAAACATCTA TTCAGTCTAC GAATGAAGAT TTGATAAAAG AAATATGGAA ATGTATTAAA 
TCAAATTTAA ACACTTCATC AACGAATGTT CTTTTATCTG CACTTCGTTG CGCTTACTAC 
ATTCTGGGCT CTTCACGTCT TCGTAATTCA GAATTGGAAT GGTTCAAATC TTTTCTATTT 
AAAAAATCAT CAATTGATAA TTCTTTGCTC CATTCTTCTT TAGGAAGATG CTTTTCTGCA 
CTTGCAAGTG CTCAAGCTGA CAAATCGATT GAAGCCGCTG CAAACGCAGT ACCCTCAACT 
CCAGCGGATG ATGAGAACAA TGAGGAAGTA GAAAATCGGT CTACTACTCG CCGTTTTGAT 
GAAACTGAGA GTTTTCGTCA CGCCCTACGG ATTCTTGTCG AAAATTATCA CTCCAAGCAT 
GCAAAGTATG AAAGCTCAAA AGCGGTTCTT GTTTTTGCCG TTAAGCATCT TTTTCTAATG 
CAACGTCCAT CCTTTGTTTC TCTCAACTAC ACTACTATAG TGGATATTAT TCTATTACAC 
CAATCACACT CTACTTCAAA AGAATGGTTG TCTTCGTTGG AGCAATATCA TTGTACATAC 
TTGTTTCGCG ATGTGATTGG AAGGCGATTT CTCGATCAAA GCTCTGAATT GCAGGGAATT 
GAAATACTTT TAAAGAGAGT GTTAGAGCCT TATTTAAAAG ATCAGTCGAA GATTTCGGAA 
ATTGCTGTTA AGACTGCGAT GGCTTCCACC GCTGATATAT TAAGCCGATC AAAGGGTTCG 
GCTACTGCTT TATATAGTCA GTTACTAGAT ACTACACTAC AGATTCTATA CTTGGGTAAA 
GATTCATTGA AGAATGAAGT AGCCTGGTTG CTGCGCTGTT TACTATCCGT TATGCCTTCT 
CCTTTAATGC CCACTTTGCT TGCTCTTTTT AATAGATTAG AGTTGGAATT TAAGCACCTT 
CGTTCTAAAC CTAAGGAAGT AAGTCCCAAA TTGAATGTTG GTATTTTCAA TAATCTTACT 
TTGTCGTTGA TGTCGATTTA TGTTGCCGCC GAAGAAAATC CTCTATATGT TGCTTCTTCT 
ATATTTTCAA AGTTATTTAA CTTCTCTTTG GATTTGATTA AGTCATCATC TAAATTGGAT 
TTATTAGCGG CACAAGCACA AATCAAGACT GGTTGGCGAC TATTAAGCGC TTACGTTTCT 
TTTGGTCCTT CTTTTACTAG AATGCAATTG TCCCAATTGC TAATTTTATG GAAGAATGCC 
CTTTCCAGAG TACCTTCAAA AATACATTCA CAAAATTTCA TGGAGACAAA TGCAGACATG 
TTTAATCGGT TTGAAGCGTT ACAGTGCTTG CTTTCGTTTT TGGAGTATAA CAAGATTTTA 
TTCACACCTG ATGTTAGGAA GCGAGTCATT ATTTTCCTAA ATAACTGCCT TAAATTCTAC 
AAAGCTTTTT CGTTAACCAA AAGGCTTGAA ATTACCATGC CAATTCCCGC TTCAGATTAT 
TCTCACTTGG AACTTCGCTC TCTCTTGCTT GCTCGGATTT TCCAATGTTT CTTCGAGTTG 
TCTCAGTTAA GTGGAATGAG TTTAGATGAT CAAGAATTAC TATCCACGAC TGTGTCTACT 
ATAGCCGAAC TTTCTTCTTT ACAAGGATAT GATACTGCGG ACGAAATAGT TCAAGCGAAA 
GCCTTTACTT CTCCAAGAGT TATACCTGGA ATTGGTTCAC GATTTTACGC TCAGCCAGAG 
GAAGAATTTC AAACAACCAT GGATGATATA CTTCCTTGTT ATATTTGCGA GTCATCTTCT 
GATTCCCTGT CAGTGATTTC GAGATCAAAC TCTGCAAAAC TTCCTTACGT ACCCCCGTCT 
TTAACTTCTT TAATTGATAA CGCGATCCAG TTATTTGCGT CTATGTTTTG TCATCAACCT 
CCGAAGGTTA TGGAGAGCAC TCTCGAAATT ATGGTAGCGT CTGTGACAAA CCTCAAGAGT 
GGGAGAGATA TTTTACGTTA CAAGGCAGTA ACAGCAAATA CTTTATTTTC CATTTATGGA 
TTTCTCTCTG CTTTCGATAA CAAAAAAGTT ACCTTGCCTG ACAAGGTTGT ATCCCTTATG 
ATAGAATTGC TCGAAAACTT GACTGCTGTT AACGATCCGA AGTTAATTCC TTACATTTCG 
AAATCTTATG CGTTTTTGGC ATATTTATAT CAAAGCAAAC CGACGTCCGT TTTGGATACA 
ATAATTAAAC GTGCTGCAGA GTCGGGAGAT CCAAATTGCA GGGCTGTCTT GATTCTAGCC 
GTTGGCCATA TATTAAAAAA GGTTGGTTCC GGAGTTCCCC AAACTACCCT CATCACCGCT 
TATCAGCTGC TCAAAGTTTT TAATACTGAT TCGAATGACC TTGTTAGAGA GTGTTCTATT 
CGTGCTTCTT CCGTTTCTCT TGGGTCTATC GCAAAGACCA TTCCGAGTGT TTTAACTGCC 
CAGACACGCA TTTTATTGAG CTATATGCTA TTGCCAGAAT GTGATATTGA TGTTCAGCTC 
TTAAAGAAGA GAAACAGTTT ATTTTTCCCG TCCACTCTTT TTGCCAATTA CTTGGATTGT 
TTGATAAATG AGCTTGGACC AAACTTGCGA TCCGATTCTT CTACTTCGCA TTTCGCATTC 
GATTTAGCTC ACCAATTGCT TTTACTTAGT AGAGATACGG GAGATAACAT TATACACGTT 
TACAAATGTT ATCAGCACCT ATATCTATTT ACACCATTAG AAACCAATTC TGATTTCTTC 
ATTAGGGAGC TTTGTGGTAA CATAATTGAC CTAAAGCAAC CTAAACGTAG GGAAATTTCT 
ATTGAAGTTT TATACCAAAT ATTGCTTCAA AACCATGAGT TCTCGGAATC ATGTTATAAG 
CGACATTTTG ATAGGCTTAT TTGGCAATCA CTCGACACCA CTCCTGACAA CAAGCTTTTG 
AAGAACATAC TGTATATCTG GCTAAACGAT TACAAAGATT CGGACATACA CTCATGGGTA 
AATATTTTAC TTTTTCTTGT CGGCATAAAA AATACTTCGA GTTCTGAAAG ATCCAACGAA 
CAAGATATAC AAAACAACCT AGATGAAGAT ACCGTATCTT TGGCTTATGG AACTTCCGAA 
AACGCAAGTA ATCCATTATT CCAGAAGAAT TACCGTTGGC AGACGCAGTT TTATGCAGCT 
ACATGCCTTA AAACATGTAT CTCTGAAGTG ATGGCCGAAA ATAAAGAGCC CGCAAAATAC 
TTGATTTCTA GGATATCTGA CCTAGTCCGT GCTGCTTTTG TTTTGTCTAC TTCAGACAAC 
TTTTACTTGA AGCAGGGGGG TTTTGAACTT TTGGATACTA TCATAACGGA CTTTTCGAAT 
GTTATGGATC CTGACTTTGA CGATGTTTCT CTTTTGGACC AATTTCAAGC TCAAATCAGC 
TCTGCTTTTA GTTCCGCGTT TGTAGATGAT TCATCTCCTG AAATAGTAGC CATGTCTCTT 
AATATAGCTT CCAATTTTAT TGGAACTGGA CTTCTAAAAA CCGAATCGCA AGCAGCTAAA 
ATCCTCAATT TATTGAAAGA AGCGTTGGAA TCTAGTTTAC CAGAGAGGGC TGGATTTGAG 
GAAAATCGTC ACTTTAAGTA TGACTCGAAA TATTTTCTTC GTATTGCTAC TTTATCAGCT 
TGGGCTTCTT TGCTGGTACA TTCAGCGAAT GTGTCATATT TGAAAGAATT TCTTTCCTCT 
AACATCCGAA GGCTATCTTC GCATTGGATT ATGGCTTTAA GAGGATATTC CAGATTGCAA 
TTTGGTCCTA GTTTAGTAAA AGATTTTGTA GCTGATACCT CTTTCACTCG GGTCCAAAGT 
ATAAACCCTA AAATACTTCT CAATATTTAT GAGAAATCTT GGCTGAACAT CGCAGAGTCA 
TTAACAATAT TACTTTCTAC TGATGCAAAT CTGATTTATG GAATATTAAG TGGAGATGAG 
CTGTCGCTTT CTGAGGAGGA CGTATTCTTC CAAGACAACA TAAATTATGC TTCCAACAGG 
TACTCTTTTA ACTTTTTTTT GTTTAGTGTC TGCTTCCAAT CTCTCTTAAT TCCAAGCACT 
TTGCAAGGCT TTAGAAATCC AGTTTTCAGA ATTATGCGAA TTATGACTGA AGTTTTAACT 
GAAGAATTAT GTACCAAGGT TCTTTATGAC CATAATGTGT TCCCCGAAGT TGTTGATCTT 
TTAGTTCGTT TGATTTTGAC GGAGGATTGG GAAACGAAGG CTAGTATTGT CATTTTTGTG 
AAAAAGTTAG CTCTTGCTAA CCCGGCACAC AATGACTTGA AGTTTTGTGA AACAGATTCT 
GTAACATCTA TGGAGCCTAC TGTATCAGAA AGCGTCGAAA TGCTTTTTCA ATTGGTCAAA 
ATATTGACAT TAGCTTTAAC GGTTAGAGTG CCGGGCCTAC GTTCTGATCC AGCTGAATCA 
GCTACCAATA AAAAAGCTAG TGAATTTGTT ATTTTGTGTT TTAATGCATT TCTTGATGTT 
TCCAACATTT TCCCCGCTAT TGTGCGTGTT GATTTATTTG CGACAGCAAT GCATGTTTAT 
GAAGTCATTA TGGACGATCA ACAACTATTA AAAAATTCTA AGCAAGAGTT GTTAGCTGCT 
CTTAGAAAGT TGTTGAGTAC AATGGTTGAG CAAAATGATC ACACATGTAC AACTTTGATA 
TACACTCTTT TTGAACACCT CCTTAACATC TACAAAGAAA CGATTAATGA TAGTGATAAA 
AAGGAGAAAA ACGAAACTGC TTTTATGAGC ATGGTCCTTG TACTTACTCT TGCAGCTCCT 
ATATTAGACA GTGAGCAGCT TGTGGTACAC GATTTTTTGG AAGAACTATT CTCTTCCTTG 
AAGAGCTCAG AAGAGGACTT TGCTTTAAGA ACTCGTTGTG TAACATCGCT GATGTTAGTT 
AACAGTAAAC ACCCAAGCAT GAGCGCTCTT TGCCGTTTCA TTATTTACAA ATCGGTTACG 
ATGCTACAGA ACGGTGAAGT TCTGAAAAGT GGTGATTATG TCACGGAGTT TATACCTGCA 
TTGCTTGATA TGCATGTCCA TATTCCGGAG GAGAAGAAAA AATCATTTTT AAGCATGTCT 
ATTCCGATAG CTGCACATTT TAGTATCCTC GTCGAGCACG CTGACGCACG AAAAAGAATC 
GGTGAAAAAC TGCTGAAGAT TATGGCTATT CAGCCAGATG AGGCCAAAGC AATCATTCAA 
CAACTTTCTC CTAGGCAAAA AAAATGCGTT GAAGAGATCT TAATACAAAA TGTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIP1 ( NM_001021451.2 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_595540.1 Schizosaccharomyces pombe 972h- Pof6 interacting protein Sip1, predicted AP-1 accessory protein (sip1), mRNA.

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CloneID	OSj01612
Clone ID Related Accession (Same CDS sequence)	NM_001021451.2
Accession Version	NM_001021451.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	Pof6 interacting protein Sip1, predicted AP-1 accessory protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021451.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGTCGTTAG CATCATTGCC GCTCGAAAAG TTGACGAAAG TAGCTGATGT AGATAAGAGG 
GAGACCATAT ATATTGATTT ACTCAATTAC GAAAAACAAT TGAGGAATCT GAACAAAAAT 
ACTCGCGAGG ATTCATTACA AACTAATTTA AATCCCCTAC TTTCGTTAAT CAAAAAATAT 
GGAAATTTTA TTGCTTATCC AACTAGAAAA CTTCTTTCTA ACTGTATAAT TGCACTCTTA 
AAAGGACTCG AGGGTTATCA TCAGCATCGA GTTTTACTTT ACTTCGTGGA TAGCATTCAT 
GAGAAAAAAG ACTATATAGA GATTATTAAC GTTTTATCCG TTCTGACTTC TTGCTTGGAA 
GAATATGGAA ATCAGATCAA TCAGCAAGAG ACTTTTATAA GATTTTTCTT CAAATATTCG 
AAAACCAATT TGTCATATGG AGTTCGCTCA ATCGCATTAG ATGGACTGTC GAAATGCATT 
GAAACATCTA TTCAGTCTAC GAATGAAGAT TTGATAAAAG AAATATGGAA ATGTATTAAA 
TCAAATTTAA ACACTTCATC AACGAATGTT CTTTTATCTG CACTTCGTTG CGCTTACTAC 
ATTCTGGGCT CTTCACGTCT TCGTAATTCA GAATTGGAAT GGTTCAAATC TTTTCTATTT 
AAAAAATCAT CAATTGATAA TTCTTTGCTC CATTCTTCTT TAGGAAGATG CTTTTCTGCA 
CTTGCAAGTG CTCAAGCTGA CAAATCGATT GAAGCCGCTG CAAACGCAGT ACCCTCAACT 
CCAGCGGATG ATGAGAACAA TGAGGAAGTA GAAAATCGGT CTACTACTCG CCGTTTTGAT 
GAAACTGAGA GTTTTCGTCA CGCCCTACGG ATTCTTGTCG AAAATTATCA CTCCAAGCAT 
GCAAAGTATG AAAGCTCAAA AGCGGTTCTT GTTTTTGCCG TTAAGCATCT TTTTCTAATG 
CAACGTCCAT CCTTTGTTTC TCTCAACTAC ACTACTATAG TGGATATTAT TCTATTACAC 
CAATCACACT CTACTTCAAA AGAATGGTTG TCTTCGTTGG AGCAATATCA TTGTACATAC 
TTGTTTCGCG ATGTGATTGG AAGGCGATTT CTCGATCAAA GCTCTGAATT GCAGGGAATT 
GAAATACTTT TAAAGAGAGT GTTAGAGCCT TATTTAAAAG ATCAGTCGAA GATTTCGGAA 
ATTGCTGTTA AGACTGCGAT GGCTTCCACC GCTGATATAT TAAGCCGATC AAAGGGTTCG 
GCTACTGCTT TATATAGTCA GTTACTAGAT ACTACACTAC AGATTCTATA CTTGGGTAAA 
GATTCATTGA AGAATGAAGT AGCCTGGTTG CTGCGCTGTT TACTATCCGT TATGCCTTCT 
CCTTTAATGC CCACTTTGCT TGCTCTTTTT AATAGATTAG AGTTGGAATT TAAGCACCTT 
CGTTCTAAAC CTAAGGAAGT AAGTCCCAAA TTGAATGTTG GTATTTTCAA TAATCTTACT 
TTGTCGTTGA TGTCGATTTA TGTTGCCGCC GAAGAAAATC CTCTATATGT TGCTTCTTCT 
ATATTTTCAA AGTTATTTAA CTTCTCTTTG GATTTGATTA AGTCATCATC TAAATTGGAT 
TTATTAGCGG CACAAGCACA AATCAAGACT GGTTGGCGAC TATTAAGCGC TTACGTTTCT 
TTTGGTCCTT CTTTTACTAG AATGCAATTG TCCCAATTGC TAATTTTATG GAAGAATGCC 
CTTTCCAGAG TACCTTCAAA AATACATTCA CAAAATTTCA TGGAGACAAA TGCAGACATG 
TTTAATCGGT TTGAAGCGTT ACAGTGCTTG CTTTCGTTTT TGGAGTATAA CAAGATTTTA 
TTCACACCTG ATGTTAGGAA GCGAGTCATT ATTTTCCTAA ATAACTGCCT TAAATTCTAC 
AAAGCTTTTT CGTTAACCAA AAGGCTTGAA ATTACCATGC CAATTCCCGC TTCAGATTAT 
TCTCACTTGG AACTTCGCTC TCTCTTGCTT GCTCGGATTT TCCAATGTTT CTTCGAGTTG 
TCTCAGTTAA GTGGAATGAG TTTAGATGAT CAAGAATTAC TATCCACGAC TGTGTCTACT 
ATAGCCGAAC TTTCTTCTTT ACAAGGATAT GATACTGCGG ACGAAATAGT TCAAGCGAAA 
GCCTTTACTT CTCCAAGAGT TATACCTGGA ATTGGTTCAC GATTTTACGC TCAGCCAGAG 
GAAGAATTTC AAACAACCAT GGATGATATA CTTCCTTGTT ATATTTGCGA GTCATCTTCT 
GATTCCCTGT CAGTGATTTC GAGATCAAAC TCTGCAAAAC TTCCTTACGT ACCCCCGTCT 
TTAACTTCTT TAATTGATAA CGCGATCCAG TTATTTGCGT CTATGTTTTG TCATCAACCT 
CCGAAGGTTA TGGAGAGCAC TCTCGAAATT ATGGTAGCGT CTGTGACAAA CCTCAAGAGT 
GGGAGAGATA TTTTACGTTA CAAGGCAGTA ACAGCAAATA CTTTATTTTC CATTTATGGA 
TTTCTCTCTG CTTTCGATAA CAAAAAAGTT ACCTTGCCTG ACAAGGTTGT ATCCCTTATG 
ATAGAATTGC TCGAAAACTT GACTGCTGTT AACGATCCGA AGTTAATTCC TTACATTTCG 
AAATCTTATG CGTTTTTGGC ATATTTATAT CAAAGCAAAC CGACGTCCGT TTTGGATACA 
ATAATTAAAC GTGCTGCAGA GTCGGGAGAT CCAAATTGCA GGGCTGTCTT GATTCTAGCC 
GTTGGCCATA TATTAAAAAA GGTTGGTTCC GGAGTTCCCC AAACTACCCT CATCACCGCT 
TATCAGCTGC TCAAAGTTTT TAATACTGAT TCGAATGACC TTGTTAGAGA GTGTTCTATT 
CGTGCTTCTT CCGTTTCTCT TGGGTCTATC GCAAAGACCA TTCCGAGTGT TTTAACTGCC 
CAGACACGCA TTTTATTGAG CTATATGCTA TTGCCAGAAT GTGATATTGA TGTTCAGCTC 
TTAAAGAAGA GAAACAGTTT ATTTTTCCCG TCCACTCTTT TTGCCAATTA CTTGGATTGT 
TTGATAAATG AGCTTGGACC AAACTTGCGA TCCGATTCTT CTACTTCGCA TTTCGCATTC 
GATTTAGCTC ACCAATTGCT TTTACTTAGT AGAGATACGG GAGATAACAT TATACACGTT 
TACAAATGTT ATCAGCACCT ATATCTATTT ACACCATTAG AAACCAATTC TGATTTCTTC 
ATTAGGGAGC TTTGTGGTAA CATAATTGAC CTAAAGCAAC CTAAACGTAG GGAAATTTCT 
ATTGAAGTTT TATACCAAAT ATTGCTTCAA AACCATGAGT TCTCGGAATC ATGTTATAAG 
CGACATTTTG ATAGGCTTAT TTGGCAATCA CTCGACACCA CTCCTGACAA CAAGCTTTTG 
AAGAACATAC TGTATATCTG GCTAAACGAT TACAAAGATT CGGACATACA CTCATGGGTA 
AATATTTTAC TTTTTCTTGT CGGCATAAAA AATACTTCGA GTTCTGAAAG ATCCAACGAA 
CAAGATATAC AAAACAACCT AGATGAAGAT ACCGTATCTT TGGCTTATGG AACTTCCGAA 
AACGCAAGTA ATCCATTATT CCAGAAGAAT TACCGTTGGC AGACGCAGTT TTATGCAGCT 
ACATGCCTTA AAACATGTAT CTCTGAAGTG ATGGCCGAAA ATAAAGAGCC CGCAAAATAC 
TTGATTTCTA GGATATCTGA CCTAGTCCGT GCTGCTTTTG TTTTGTCTAC TTCAGACAAC 
TTTTACTTGA AGCAGGGGGG TTTTGAACTT TTGGATACTA TCATAACGGA CTTTTCGAAT 
GTTATGGATC CTGACTTTGA CGATGTTTCT CTTTTGGACC AATTTCAAGC TCAAATCAGC 
TCTGCTTTTA GTTCCGCGTT TGTAGATGAT TCATCTCCTG AAATAGTAGC CATGTCTCTT 
AATATAGCTT CCAATTTTAT TGGAACTGGA CTTCTAAAAA CCGAATCGCA AGCAGCTAAA 
ATCCTCAATT TATTGAAAGA AGCGTTGGAA TCTAGTTTAC CAGAGAGGGC TGGATTTGAG 
GAAAATCGTC ACTTTAAGTA TGACTCGAAA TATTTTCTTC GTATTGCTAC TTTATCAGCT 
TGGGCTTCTT TGCTGGTACA TTCAGCGAAT GTGTCATATT TGAAAGAATT TCTTTCCTCT 
AACATCCGAA GGCTATCTTC GCATTGGATT ATGGCTTTAA GAGGATATTC CAGATTGCAA 
TTTGGTCCTA GTTTAGTAAA AGATTTTGTA GCTGATACCT CTTTCACTCG GGTCCAAAGT 
ATAAACCCTA AAATACTTCT CAATATTTAT GAGAAATCTT GGCTGAACAT CGCAGAGTCA 
TTAACAATAT TACTTTCTAC TGATGCAAAT CTGATTTATG GAATATTAAG TGGAGATGAG 
CTGTCGCTTT CTGAGGAGGA CGTATTCTTC CAAGACAACA TAAATTATGC TTCCAACAGG 
TACTCTTTTA ACTTTTTTTT GTTTAGTGTC TGCTTCCAAT CTCTCTTAAT TCCAAGCACT 
TTGCAAGGCT TTAGAAATCC AGTTTTCAGA ATTATGCGAA TTATGACTGA AGTTTTAACT 
GAAGAATTAT GTACCAAGGT TCTTTATGAC CATAATGTGT TCCCCGAAGT TGTTGATCTT 
TTAGTTCGTT TGATTTTGAC GGAGGATTGG GAAACGAAGG CTAGTATTGT CATTTTTGTG 
AAAAAGTTAG CTCTTGCTAA CCCGGCACAC AATGACTTGA AGTTTTGTGA AACAGATTCT 
GTAACATCTA TGGAGCCTAC TGTATCAGAA AGCGTCGAAA TGCTTTTTCA ATTGGTCAAA 
ATATTGACAT TAGCTTTAAC GGTTAGAGTG CCGGGCCTAC GTTCTGATCC AGCTGAATCA 
GCTACCAATA AAAAAGCTAG TGAATTTGTT ATTTTGTGTT TTAATGCATT TCTTGATGTT 
TCCAACATTT TCCCCGCTAT TGTGCGTGTT GATTTATTTG CGACAGCAAT GCATGTTTAT 
GAAGTCATTA TGGACGATCA ACAACTATTA AAAAATTCTA AGCAAGAGTT GTTAGCTGCT 
CTTAGAAAGT TGTTGAGTAC AATGGTTGAG CAAAATGATC ACACATGTAC AACTTTGATA 
TACACTCTTT TTGAACACCT CCTTAACATC TACAAAGAAA CGATTAATGA TAGTGATAAA 
AAGGAGAAAA ACGAAACTGC TTTTATGAGC ATGGTCCTTG TACTTACTCT TGCAGCTCCT 
ATATTAGACA GTGAGCAGCT TGTGGTACAC GATTTTTTGG AAGAACTATT CTCTTCCTTG 
AAGAGCTCAG AAGAGGACTT TGCTTTAAGA ACTCGTTGTG TAACATCGCT GATGTTAGTT 
AACAGTAAAC ACCCAAGCAT GAGCGCTCTT TGCCGTTTCA TTATTTACAA ATCGGTTACG 
ATGCTACAGA ACGGTGAAGT TCTGAAAAGT GGTGATTATG TCACGGAGTT TATACCTGCA 
TTGCTTGATA TGCATGTCCA TATTCCGGAG GAGAAGAAAA AATCATTTTT AAGCATGTCT 
ATTCCGATAG CTGCACATTT TAGTATCCTC GTCGAGCACG CTGACGCACG AAAAAGAATC 
GGTGAAAAAC TGCTGAAGAT TATGGCTATT CAGCCAGATG AGGCCAAAGC AATCATTCAA 
CAACTTTCTC CTAGGCAAAA AAAATGCGTT GAAGAGATCT TAATACAAAA TGTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595540.1
CDS	36..5795
Translation	MSLASLPLEKLTKVADVDKRETIYIDLLNYEKQLRNLNKNTREDSLQTNLNPLLSLIKKYGNFIAYPTRKLLSNCIIALLKGLEGYHQHRVLLYFVDSIHEKKDYIEIINVLSVLTSCLEEYGNQINQQETFIRFFFKYSKTNLSYGVRSIALDGLSKCIETSIQSTNEDLIKEIWKCIKSNLNTSSTNVLLSALRCAYYILGSSRLRNSELEWFKSFLFKKSSIDNSLLHSSLGRCFSALASAQADKSIEAAANAVPSTPADDENNEEVENRSTTRRFDETESFRHALRILVENYHSKHAKYESSKAVLVFAVKHLFLMQRPSFVSLNYTTIVDIILLHQSHSTSKEWLSSLEQYHCTYLFRDVIGRRFLDQSSELQGIEILLKRVLEPYLKDQSKISEIAVKTAMASTADILSRSKGSATALYSQLLDTTLQILYLGKDSLKNEVAWLLRCLLSVMPSPLMPTLLALFNRLELEFKHLRSKPKEVSPKLNVGIFNNLTLSLMSIYVAAEENPLYVASSIFSKLFNFSLDLIKSSSKLDLLAAQAQIKTGWRLLSAYVSFGPSFTRMQLSQLLILWKNALSRVPSKIHSQNFMETNADMFNRFEALQCLLSFLEYNKILFTPDVRKRVIIFLNNCLKFYKAFSLTKRLEITMPIPASDYSHLELRSLLLARIFQCFFELSQLSGMSLDDQELLSTTVSTIAELSSLQGYDTADEIVQAKAFTSPRVIPGIGSRFYAQPEEEFQTTMDDILPCYICESSSDSLSVISRSNSAKLPYVPPSLTSLIDNAIQLFASMFCHQPPKVMESTLEIMVASVTNLKSGRDILRYKAVTANTLFSIYGFLSAFDNKKVTLPDKVVSLMIELLENLTAVNDPKLIPYISKSYAFLAYLYQSKPTSVLDTIIKRAAESGDPNCRAVLILAVGHILKKVGSGVPQTTLITAYQLLKVFNTDSNDLVRECSIRASSVSLGSIAKTIPSVLTAQTRILLSYMLLPECDIDVQLLKKRNSLFFPSTLFANYLDCLINELGPNLRSDSSTSHFAFDLAHQLLLLSRDTGDNIIHVYKCYQHLYLFTPLETNSDFFIRELCGNIIDLKQPKRREISIEVLYQILLQNHEFSESCYKRHFDRLIWQSLDTTPDNKLLKNILYIWLNDYKDSDIHSWVNILLFLVGIKNTSSSERSNEQDIQNNLDEDTVSLAYGTSENASNPLFQKNYRWQTQFYAATCLKTCISEVMAENKEPAKYLISRISDLVRAAFVLSTSDNFYLKQGGFELLDTIITDFSNVMDPDFDDVSLLDQFQAQISSAFSSAFVDDSSPEIVAMSLNIASNFIGTGLLKTESQAAKILNLLKEALESSLPERAGFEENRHFKYDSKYFLRIATLSAWASLLVHSANVSYLKEFLSSNIRRLSSHWIMALRGYSRLQFGPSLVKDFVADTSFTRVQSINPKILLNIYEKSWLNIAESLTILLSTDANLIYGILSGDELSLSEEDVFFQDNINYASNRYSFNFFLFSVCFQSLLIPSTLQGFRNPVFRIMRIMTEVLTEELCTKVLYDHNVFPEVVDLLVRLILTEDWETKASIVIFVKKLALANPAHNDLKFCETDSVTSMEPTVSESVEMLFQLVKILTLALTVRVPGLRSDPAESATNKKASEFVILCFNAFLDVSNIFPAIVRVDLFATAMHVYEVIMDDQQLLKNSKQELLAALRKLLSTMVEQNDHTCTTLIYTLFEHLLNIYKETINDSDKKEKNETAFMSMVLVLTLAAPILDSEQLVVHDFLEELFSSLKSSEEDFALRTRCVTSLMLVNSKHPSMSALCRFIIYKSVTMLQNGEVLKSGDYVTEFIPALLDMHVHIPEEKKKSFLSMSIPIAAHFSILVEHADARKRIGEKLLKIMAIQPDEAKAIIQQLSPRQKKCVEEILIQNVE

Target ORF information:

RefSeq Version	NM_001021451.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- Pof6 interacting protein Sip1, predicted AP-1 accessory protein (sip1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021451.2

ORF Insert Sequence:

ATGTCGTTAG CATCATTGCC GCTCGAAAAG TTGACGAAAG TAGCTGATGT AGATAAGAGG 
GAGACCATAT ATATTGATTT ACTCAATTAC GAAAAACAAT TGAGGAATCT GAACAAAAAT 
ACTCGCGAGG ATTCATTACA AACTAATTTA AATCCCCTAC TTTCGTTAAT CAAAAAATAT 
GGAAATTTTA TTGCTTATCC AACTAGAAAA CTTCTTTCTA ACTGTATAAT TGCACTCTTA 
AAAGGACTCG AGGGTTATCA TCAGCATCGA GTTTTACTTT ACTTCGTGGA TAGCATTCAT 
GAGAAAAAAG ACTATATAGA GATTATTAAC GTTTTATCCG TTCTGACTTC TTGCTTGGAA 
GAATATGGAA ATCAGATCAA TCAGCAAGAG ACTTTTATAA GATTTTTCTT CAAATATTCG 
AAAACCAATT TGTCATATGG AGTTCGCTCA ATCGCATTAG ATGGACTGTC GAAATGCATT 
GAAACATCTA TTCAGTCTAC GAATGAAGAT TTGATAAAAG AAATATGGAA ATGTATTAAA 
TCAAATTTAA ACACTTCATC AACGAATGTT CTTTTATCTG CACTTCGTTG CGCTTACTAC 
ATTCTGGGCT CTTCACGTCT TCGTAATTCA GAATTGGAAT GGTTCAAATC TTTTCTATTT 
AAAAAATCAT CAATTGATAA TTCTTTGCTC CATTCTTCTT TAGGAAGATG CTTTTCTGCA 
CTTGCAAGTG CTCAAGCTGA CAAATCGATT GAAGCCGCTG CAAACGCAGT ACCCTCAACT 
CCAGCGGATG ATGAGAACAA TGAGGAAGTA GAAAATCGGT CTACTACTCG CCGTTTTGAT 
GAAACTGAGA GTTTTCGTCA CGCCCTACGG ATTCTTGTCG AAAATTATCA CTCCAAGCAT 
GCAAAGTATG AAAGCTCAAA AGCGGTTCTT GTTTTTGCCG TTAAGCATCT TTTTCTAATG 
CAACGTCCAT CCTTTGTTTC TCTCAACTAC ACTACTATAG TGGATATTAT TCTATTACAC 
CAATCACACT CTACTTCAAA AGAATGGTTG TCTTCGTTGG AGCAATATCA TTGTACATAC 
TTGTTTCGCG ATGTGATTGG AAGGCGATTT CTCGATCAAA GCTCTGAATT GCAGGGAATT 
GAAATACTTT TAAAGAGAGT GTTAGAGCCT TATTTAAAAG ATCAGTCGAA GATTTCGGAA 
ATTGCTGTTA AGACTGCGAT GGCTTCCACC GCTGATATAT TAAGCCGATC AAAGGGTTCG 
GCTACTGCTT TATATAGTCA GTTACTAGAT ACTACACTAC AGATTCTATA CTTGGGTAAA 
GATTCATTGA AGAATGAAGT AGCCTGGTTG CTGCGCTGTT TACTATCCGT TATGCCTTCT 
CCTTTAATGC CCACTTTGCT TGCTCTTTTT AATAGATTAG AGTTGGAATT TAAGCACCTT 
CGTTCTAAAC CTAAGGAAGT AAGTCCCAAA TTGAATGTTG GTATTTTCAA TAATCTTACT 
TTGTCGTTGA TGTCGATTTA TGTTGCCGCC GAAGAAAATC CTCTATATGT TGCTTCTTCT 
ATATTTTCAA AGTTATTTAA CTTCTCTTTG GATTTGATTA AGTCATCATC TAAATTGGAT 
TTATTAGCGG CACAAGCACA AATCAAGACT GGTTGGCGAC TATTAAGCGC TTACGTTTCT 
TTTGGTCCTT CTTTTACTAG AATGCAATTG TCCCAATTGC TAATTTTATG GAAGAATGCC 
CTTTCCAGAG TACCTTCAAA AATACATTCA CAAAATTTCA TGGAGACAAA TGCAGACATG 
TTTAATCGGT TTGAAGCGTT ACAGTGCTTG CTTTCGTTTT TGGAGTATAA CAAGATTTTA 
TTCACACCTG ATGTTAGGAA GCGAGTCATT ATTTTCCTAA ATAACTGCCT TAAATTCTAC 
AAAGCTTTTT CGTTAACCAA AAGGCTTGAA ATTACCATGC CAATTCCCGC TTCAGATTAT 
TCTCACTTGG AACTTCGCTC TCTCTTGCTT GCTCGGATTT TCCAATGTTT CTTCGAGTTG 
TCTCAGTTAA GTGGAATGAG TTTAGATGAT CAAGAATTAC TATCCACGAC TGTGTCTACT 
ATAGCCGAAC TTTCTTCTTT ACAAGGATAT GATACTGCGG ACGAAATAGT TCAAGCGAAA 
GCCTTTACTT CTCCAAGAGT TATACCTGGA ATTGGTTCAC GATTTTACGC TCAGCCAGAG 
GAAGAATTTC AAACAACCAT GGATGATATA CTTCCTTGTT ATATTTGCGA GTCATCTTCT 
GATTCCCTGT CAGTGATTTC GAGATCAAAC TCTGCAAAAC TTCCTTACGT ACCCCCGTCT 
TTAACTTCTT TAATTGATAA CGCGATCCAG TTATTTGCGT CTATGTTTTG TCATCAACCT 
CCGAAGGTTA TGGAGAGCAC TCTCGAAATT ATGGTAGCGT CTGTGACAAA CCTCAAGAGT 
GGGAGAGATA TTTTACGTTA CAAGGCAGTA ACAGCAAATA CTTTATTTTC CATTTATGGA 
TTTCTCTCTG CTTTCGATAA CAAAAAAGTT ACCTTGCCTG ACAAGGTTGT ATCCCTTATG 
ATAGAATTGC TCGAAAACTT GACTGCTGTT AACGATCCGA AGTTAATTCC TTACATTTCG 
AAATCTTATG CGTTTTTGGC ATATTTATAT CAAAGCAAAC CGACGTCCGT TTTGGATACA 
ATAATTAAAC GTGCTGCAGA GTCGGGAGAT CCAAATTGCA GGGCTGTCTT GATTCTAGCC 
GTTGGCCATA TATTAAAAAA GGTTGGTTCC GGAGTTCCCC AAACTACCCT CATCACCGCT 
TATCAGCTGC TCAAAGTTTT TAATACTGAT TCGAATGACC TTGTTAGAGA GTGTTCTATT 
CGTGCTTCTT CCGTTTCTCT TGGGTCTATC GCAAAGACCA TTCCGAGTGT TTTAACTGCC 
CAGACACGCA TTTTATTGAG CTATATGCTA TTGCCAGAAT GTGATATTGA TGTTCAGCTC 
TTAAAGAAGA GAAACAGTTT ATTTTTCCCG TCCACTCTTT TTGCCAATTA CTTGGATTGT 
TTGATAAATG AGCTTGGACC AAACTTGCGA TCCGATTCTT CTACTTCGCA TTTCGCATTC 
GATTTAGCTC ACCAATTGCT TTTACTTAGT AGAGATACGG GAGATAACAT TATACACGTT 
TACAAATGTT ATCAGCACCT ATATCTATTT ACACCATTAG AAACCAATTC TGATTTCTTC 
ATTAGGGAGC TTTGTGGTAA CATAATTGAC CTAAAGCAAC CTAAACGTAG GGAAATTTCT 
ATTGAAGTTT TATACCAAAT ATTGCTTCAA AACCATGAGT TCTCGGAATC ATGTTATAAG 
CGACATTTTG ATAGGCTTAT TTGGCAATCA CTCGACACCA CTCCTGACAA CAAGCTTTTG 
AAGAACATAC TGTATATCTG GCTAAACGAT TACAAAGATT CGGACATACA CTCATGGGTA 
AATATTTTAC TTTTTCTTGT CGGCATAAAA AATACTTCGA GTTCTGAAAG ATCCAACGAA 
CAAGATATAC AAAACAACCT AGATGAAGAT ACCGTATCTT TGGCTTATGG AACTTCCGAA 
AACGCAAGTA ATCCATTATT CCAGAAGAAT TACCGTTGGC AGACGCAGTT TTATGCAGCT 
ACATGCCTTA AAACATGTAT CTCTGAAGTG ATGGCCGAAA ATAAAGAGCC CGCAAAATAC 
TTGATTTCTA GGATATCTGA CCTAGTCCGT GCTGCTTTTG TTTTGTCTAC TTCAGACAAC 
TTTTACTTGA AGCAGGGGGG TTTTGAACTT TTGGATACTA TCATAACGGA CTTTTCGAAT 
GTTATGGATC CTGACTTTGA CGATGTTTCT CTTTTGGACC AATTTCAAGC TCAAATCAGC 
TCTGCTTTTA GTTCCGCGTT TGTAGATGAT TCATCTCCTG AAATAGTAGC CATGTCTCTT 
AATATAGCTT CCAATTTTAT TGGAACTGGA CTTCTAAAAA CCGAATCGCA AGCAGCTAAA 
ATCCTCAATT TATTGAAAGA AGCGTTGGAA TCTAGTTTAC CAGAGAGGGC TGGATTTGAG 
GAAAATCGTC ACTTTAAGTA TGACTCGAAA TATTTTCTTC GTATTGCTAC TTTATCAGCT 
TGGGCTTCTT TGCTGGTACA TTCAGCGAAT GTGTCATATT TGAAAGAATT TCTTTCCTCT 
AACATCCGAA GGCTATCTTC GCATTGGATT ATGGCTTTAA GAGGATATTC CAGATTGCAA 
TTTGGTCCTA GTTTAGTAAA AGATTTTGTA GCTGATACCT CTTTCACTCG GGTCCAAAGT 
ATAAACCCTA AAATACTTCT CAATATTTAT GAGAAATCTT GGCTGAACAT CGCAGAGTCA 
TTAACAATAT TACTTTCTAC TGATGCAAAT CTGATTTATG GAATATTAAG TGGAGATGAG 
CTGTCGCTTT CTGAGGAGGA CGTATTCTTC CAAGACAACA TAAATTATGC TTCCAACAGG 
TACTCTTTTA ACTTTTTTTT GTTTAGTGTC TGCTTCCAAT CTCTCTTAAT TCCAAGCACT 
TTGCAAGGCT TTAGAAATCC AGTTTTCAGA ATTATGCGAA TTATGACTGA AGTTTTAACT 
GAAGAATTAT GTACCAAGGT TCTTTATGAC CATAATGTGT TCCCCGAAGT TGTTGATCTT 
TTAGTTCGTT TGATTTTGAC GGAGGATTGG GAAACGAAGG CTAGTATTGT CATTTTTGTG 
AAAAAGTTAG CTCTTGCTAA CCCGGCACAC AATGACTTGA AGTTTTGTGA AACAGATTCT 
GTAACATCTA TGGAGCCTAC TGTATCAGAA AGCGTCGAAA TGCTTTTTCA ATTGGTCAAA 
ATATTGACAT TAGCTTTAAC GGTTAGAGTG CCGGGCCTAC GTTCTGATCC AGCTGAATCA 
GCTACCAATA AAAAAGCTAG TGAATTTGTT ATTTTGTGTT TTAATGCATT TCTTGATGTT 
TCCAACATTT TCCCCGCTAT TGTGCGTGTT GATTTATTTG CGACAGCAAT GCATGTTTAT 
GAAGTCATTA TGGACGATCA ACAACTATTA AAAAATTCTA AGCAAGAGTT GTTAGCTGCT 
CTTAGAAAGT TGTTGAGTAC AATGGTTGAG CAAAATGATC ACACATGTAC AACTTTGATA 
TACACTCTTT TTGAACACCT CCTTAACATC TACAAAGAAA CGATTAATGA TAGTGATAAA 
AAGGAGAAAA ACGAAACTGC TTTTATGAGC ATGGTCCTTG TACTTACTCT TGCAGCTCCT 
ATATTAGACA GTGAGCAGCT TGTGGTACAC GATTTTTTGG AAGAACTATT CTCTTCCTTG 
AAGAGCTCAG AAGAGGACTT TGCTTTAAGA ACTCGTTGTG TAACATCGCT GATGTTAGTT 
AACAGTAAAC ACCCAAGCAT GAGCGCTCTT TGCCGTTTCA TTATTTACAA ATCGGTTACG 
ATGCTACAGA ACGGTGAAGT TCTGAAAAGT GGTGATTATG TCACGGAGTT TATACCTGCA 
TTGCTTGATA TGCATGTCCA TATTCCGGAG GAGAAGAAAA AATCATTTTT AAGCATGTCT 
ATTCCGATAG CTGCACATTT TAGTATCCTC GTCGAGCACG CTGACGCACG AAAAAGAATC 
GGTGAAAAAC TGCTGAAGAT TATGGCTATT CAGCCAGATG AGGCCAAAGC AATCATTCAA 
CAACTTTCTC CTAGGCAAAA AAAATGCGTT GAAGAGATCT TAATACAAAA TGTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SIP1 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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