BEM3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BEM3
Entrez Gene ID	855988
Full Name	Bem3p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183929.1	NP_015210.1	Bem3p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	BEM3	NP_015210.1
Kluyveromyces lactis	KLLA0F00814g	XP_455116.1
Eremothecium gossypii	AGOS_AGR230W	NP_986896.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We find that Kss1 also localizes to the sites of polarized growth and Bem3 interacts with Kss1 at the septin-ring assembly site. TD2 localization is largely mediated by its interaction with the polarisome component Epo1 via heterotypic coiled-coil interaction. This finding reveals a novel role for the polarisome in linking Bem3 to its functional target,Cdc42.
Title: Roles of an N-terminal coiled-coil-containing domain in the localization and function of Bem3, a Rho GTPase-activating protein in budding yeast.

Bem2p and Bem3p may function as global inhibitors of Cdc42p activation during G1, and their inactivation by the Cdc28p/Cln kinase contributes to site-specific activation of Cdc42p at bud emergence
Title: Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence.

The following BEM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BEM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04857	NM_001183929.1 Latest version!	Saccharomyces cerevisiae S288C Bem3p (BEM3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BEM3 ( NM_001183929.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015210.1 Saccharomyces cerevisiae S288C Bem3p (BEM3), partial mRNA.

Price & Availability↑

CloneID	OSi04857
Clone ID Related Accession (Same CDS sequence)	NM_001183929.1
Accession Version	NM_001183929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bem3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACAGATA ATTTGACCAC AACTCATGGT GGTAGTACCA CCCTTGAATT GTTGGCGCAG 
TACAATGATC ATAGATCCAA AAAGGACAAA TCTATAGAAC ATATAGAAAA AGGAACATGT 
TCAGGAAAAG AAAGAAATCC GTCCTATGAT GAAATTTTTA CGGAAAACAT TAAGCTAAAG 
CTACAAGTCC AAGAATATGA AACTGAAATT GAGAGTTTGG AAAAGGTTAT TGATATGTTA 
CAAAAAAACA GAGAAGCTTC CTTAGAAGTT GTGCTTGAGC AAGTTCAAAA TGACTCAAGA 
GATTCATACG TTAACGATCA ATCATTTGTG TTACCCCCAA GATCTGCTGA AAGGAAAGCG 
CATATCAAGA GTCTTAATTT GCCAATACCG ACATTGTCCC CTCCATTGCA ACAAGGTTCA 
GACGTTGCAC TTGAAACAAG TGTAACGCCT ACAGTACCTC AAATAGGTGT TACATCAAAT 
ACTTCAATAA GTAGGAAGCA TTTACAGAAC ATGATACTAA ACGATGAAAT AGAAGCAAAT 
AGTAGCTTCT CATCTCCCAA AATTATCAAT AGATCTGTCT CAAGCCCTAC AAAAATACAT 
TCAGAACAGT TGGCTAGCCC AGCTGCCTCT GTTACCTATA CGACGTCTAG GATAACCATA 
AAATCACCTA ATAAAGGTTC GAAATCTCCA TTGCAAGAAC GTTTGCGTTC CCCTCAAAAT 
CCTAACAGAA TGACGGCTGT TATCAACAAC CATTTGCATT CTCCATTAAA GGCCAGTACC 
TCTAATAATT TAGATGAGCT GACTGAAAGT AAAAGTCAAC AACTAACTAA TGACGCGATA 
CAAAAAAATG ACCGCGTTTA TTCTTCTATA ACATCCTCTG CATACACTAC TGGAACTCCT 
ACTTCAGCAG CCAAAAGTCC ATCATCCCTT TTGGAAGTCA AGGAGGGTGA AAACAAGGCT 
TTGGGTTTCT CTCCAGCATC TAAAGAAAAA CTTGACGACT TCACCCAATT ACTTGACTCC 
TCATTTGGCG AAGAGGATCT AGTCAATACG GACAGTAAAG ATCCTCTGTC CATAAAATCC 
ACGATTAACG AATCTTTACC CCCACCTCCA GCGCCTCCAA CTTTTTTCTC ACCTACTTCT 
AGTGGAAATA TAAAAAACTC TACTCCTTTA TCAAGCCATT TGGCTTCTCC GGTTATCTTG 
AATAAAAAGG ATGATAATTT TGGTGCTCAA AGTGCAAAAA ATTTAAAGAA ACCTGTGTTA 
ACTTCATCAC TCCCCAATCT TTCTACAAAA TTGTCAACAA CAAGTCAAAA TGCTTCTCTT 
CCTCCAAATC CTCCCGTGGA AAGTTCATCA AAACAAAAAC AACTTGGAGA AACGGCATCC 
ATCCATTCAA CCAATACCTT GAATACATTT AGCTCAACAC CCCAAGGTTC GTTGAAGACA 
CTTAGAAGAC CTCATGCAAG TAGCGTGAGC ACCGTGAAGT CTGTGGCCCA AAGTCTTAAG 
TCGGATATTC CATTATTCGT TCAACCGGAG GATTTTGGCA CTATCCAGAT TGAAGTACTG 
AGCACATTGT ACAGGGATAA TGAAGATGAT TTATCCATTT TAATTGCAAT CATAGACAGG 
AAATCCGGTA AGGAGATGTT TAAATTTTCA AAATCTATTC ATAAAGTTCG CGAATTAGAT 
GTATATATGA AATCACATGT TCCCGATTTA CCATTGCCCA CGTTACCTGA TAGACAATTA 
TTTCAGACTT TATCACCTAC AAAAGTGGAT ACGCGAAAGA ACATATTGAA TCAGTATTAT 
ACAAGCATAT TCAGTGTCCC TGAATTTCCC AAAAATGTTG GTTTAAAAAT CGCCCAATTT 
ATTAGTACAG ATACAGTAAT GACACCTCCA ATGATGGATG ATAATGTGAA AGACGGATCA 
CTTTTGCTGA GAAGACCAAA GACTCTAACA GGTAATAGTA CATGGAGAGT TCGGTACGGT 
ATTTTGCGCG ATGATGTTTT ACAATTGTTT GACAAAAATC AGTTGACTGA AACTATTAAA 
TTGAGACAAT CTTCTATAGA ACTTATACCC AATCTCCCTG AAGATAGATT TGGAACGAGA 
AATGGGTTTT TGATCACTGA GCATAAGAAA AGCGGACTGT CAACCTCAAC AAAGTATTAT 
ATTTGTACTG AAACGTCGAA GGAACGTGAA TTATGGCTTT CTGCGTTTAG TGATTACATT 
GATCCTTCAC AAAGTTTATC GTTATCTAGC TCGAGGAATG CAAACGATAC TGATTCTGCA 
TCTCACTTGA GCGCAGGAAC ACACCATTCT AAATTTGGAA ACGCAACTAT CTCAGCAACC 
GATACTCCAT CATATGTCAC TGATTTGACC CAAGAATATA ATAATAATAA CAATATTAGT 
AATAGTAGCA ATAACATTGC TAATAGCGAT GGTATTGACT CAAACCCATC TTCTCACTCT 
AACTTCCTAG CAAGTTCAAG CGGGAATGCA GAGGAGGAGA AGGATAGTAG GAGAGCCAAA 
ATGAGAAGTT TATTCCCTTT TAAAAAATTA ACCGGCCCGG CGAGTGCAAT GAACCATATA 
GGTATTACAA TTTCAAATGA TTCGGATTCT CCAACATCTC CTGACTCTAT CATTAAGTCA 
CCAAGCAAAA AATTAATGGA AGTATCCTCT TCGTCAAATT CTTCTACGGG TCCACATGTG 
TCCACTGCAA TATTTGGCTC GTCACTAGAA ACGTGTTTAA GATTGAGTTC GCATAAGTAC 
CAAAACGTTT ACGATTTACC GAGTGTAGTT TATCGTTGCT TAGAGTATCT TTACAAAAAC 
CGTGGTATCC AGGAAGAAGG CATATTTAGA CTAAGCGGGT CCAGTACAGT TATCAAAACT 
TTACAAGAAA GGTTTGATAA AGAGTACGAT GTAGATTTAT GTCGGTACAA TGAAAGTATT 
GAAGCTAAAG ATGATGAGGC ATCTCCAAGT CTTTACATTG GTGTGAATAC TGTAAGTGGG 
TTACTAAAGT TGTACTTGAG AAAGTTGCCC CATTTATTAT TTGGTGACGA ACAATTTTTG 
TCGTTTAAAA GAGTAGTTGA TGAAAATCAT AATAATCCTG TTCAAATATC ATTGGGGTTT 
AAGGAACTTA TTGAATCTGG CCTAGTTCCT CACGCTAACC TATCGTTGAT GTATGCTCTT 
TTTGAACTGC TGGTAAGGAT CAATGAGAAT AGTAAGTTTA ATAAGATGAA TCTAAGAAAT 
TTATGCATCG TGTTTTCCCC AACTCTAAAT ATTCCAATTA GTATGCTTCA ACCATTCATC 
ACTGACTTTG CGTGTATATT TCAGGGTGGT GAACCAGTTA AAGAAGAGGA AAGGGAGAAA 
GTAGATATAC ATATTCCTCA GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015210.1
CDS	1..3387
Translation	MTDNLTTTHGGSTTLELLAQYNDHRSKKDKSIEHIEKGTCSGKERNPSYDEIFTENIKLKLQVQEYETEIESLEKVIDMLQKNREASLEVVLEQVQNDSRDSYVNDQSFVLPPRSAERKAHIKSLNLPIPTLSPPLQQGSDVALETSVTPTVPQIGVTSNTSISRKHLQNMILNDEIEANSSFSSPKIINRSVSSPTKIHSEQLASPAASVTYTTSRITIKSPNKGSKSPLQERLRSPQNPNRMTAVINNHLHSPLKASTSNNLDELTESKSQQLTNDAIQKNDRVYSSITSSAYTTGTPTSAAKSPSSLLEVKEGENKALGFSPASKEKLDDFTQLLDSSFGEEDLVNTDSKDPLSIKSTINESLPPPPAPPTFFSPTSSGNIKNSTPLSSHLASPVILNKKDDNFGAQSAKNLKKPVLTSSLPNLSTKLSTTSQNASLPPNPPVESSSKQKQLGETASIHSTNTLNTFSSTPQGSLKTLRRPHASSVSTVKSVAQSLKSDIPLFVQPEDFGTIQIEVLSTLYRDNEDDLSILIAIIDRKSGKEMFKFSKSIHKVRELDVYMKSHVPDLPLPTLPDRQLFQTLSPTKVDTRKNILNQYYTSIFSVPEFPKNVGLKIAQFISTDTVMTPPMMDDNVKDGSLLLRRPKTLTGNSTWRVRYGILRDDVLQLFDKNQLTETIKLRQSSIELIPNLPEDRFGTRNGFLITEHKKSGLSTSTKYYICTETSKERELWLSAFSDYIDPSQSLSLSSSRNANDTDSASHLSAGTHHSKFGNATISATDTPSYVTDLTQEYNNNNNISNSSNNIANSDGIDSNPSSHSNFLASSSGNAEEEKDSRRAKMRSLFPFKKLTGPASAMNHIGITISNDSDSPTSPDSIIKSPSKKLMEVSSSSNSSTGPHVSTAIFGSSLETCLRLSSHKYQNVYDLPSVVYRCLEYLYKNRGIQEEGIFRLSGSSTVIKTLQERFDKEYDVDLCRYNESIEAKDDEASPSLYIGVNTVSGLLKLYLRKLPHLLFGDEQFLSFKRVVDENHNNPVQISLGFKELIESGLVPHANLSLMYALFELLVRINENSKFNKMNLRNLCIVFSPTLNIPISMLQPFITDFACIFQGGEPVKEEEREKVDIHIPQV

Target ORF information:

RefSeq Version	NM_001183929.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bem3p (BEM3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183929.1

ORF Insert Sequence:

ATGACAGATA ATTTGACCAC AACTCATGGT GGTAGTACCA CCCTTGAATT GTTGGCGCAG 
TACAATGATC ATAGATCCAA AAAGGACAAA TCTATAGAAC ATATAGAAAA AGGAACATGT 
TCAGGAAAAG AAAGAAATCC GTCCTATGAT GAAATTTTTA CGGAAAACAT TAAGCTAAAG 
CTACAAGTCC AAGAATATGA AACTGAAATT GAGAGTTTGG AAAAGGTTAT TGATATGTTA 
CAAAAAAACA GAGAAGCTTC CTTAGAAGTT GTGCTTGAGC AAGTTCAAAA TGACTCAAGA 
GATTCATACG TTAACGATCA ATCATTTGTG TTACCCCCAA GATCTGCTGA AAGGAAAGCG 
CATATCAAGA GTCTTAATTT GCCAATACCG ACATTGTCCC CTCCATTGCA ACAAGGTTCA 
GACGTTGCAC TTGAAACAAG TGTAACGCCT ACAGTACCTC AAATAGGTGT TACATCAAAT 
ACTTCAATAA GTAGGAAGCA TTTACAGAAC ATGATACTAA ACGATGAAAT AGAAGCAAAT 
AGTAGCTTCT CATCTCCCAA AATTATCAAT AGATCTGTCT CAAGCCCTAC AAAAATACAT 
TCAGAACAGT TGGCTAGCCC AGCTGCCTCT GTTACCTATA CGACGTCTAG GATAACCATA 
AAATCACCTA ATAAAGGTTC GAAATCTCCA TTGCAAGAAC GTTTGCGTTC CCCTCAAAAT 
CCTAACAGAA TGACGGCTGT TATCAACAAC CATTTGCATT CTCCATTAAA GGCCAGTACC 
TCTAATAATT TAGATGAGCT GACTGAAAGT AAAAGTCAAC AACTAACTAA TGACGCGATA 
CAAAAAAATG ACCGCGTTTA TTCTTCTATA ACATCCTCTG CATACACTAC TGGAACTCCT 
ACTTCAGCAG CCAAAAGTCC ATCATCCCTT TTGGAAGTCA AGGAGGGTGA AAACAAGGCT 
TTGGGTTTCT CTCCAGCATC TAAAGAAAAA CTTGACGACT TCACCCAATT ACTTGACTCC 
TCATTTGGCG AAGAGGATCT AGTCAATACG GACAGTAAAG ATCCTCTGTC CATAAAATCC 
ACGATTAACG AATCTTTACC CCCACCTCCA GCGCCTCCAA CTTTTTTCTC ACCTACTTCT 
AGTGGAAATA TAAAAAACTC TACTCCTTTA TCAAGCCATT TGGCTTCTCC GGTTATCTTG 
AATAAAAAGG ATGATAATTT TGGTGCTCAA AGTGCAAAAA ATTTAAAGAA ACCTGTGTTA 
ACTTCATCAC TCCCCAATCT TTCTACAAAA TTGTCAACAA CAAGTCAAAA TGCTTCTCTT 
CCTCCAAATC CTCCCGTGGA AAGTTCATCA AAACAAAAAC AACTTGGAGA AACGGCATCC 
ATCCATTCAA CCAATACCTT GAATACATTT AGCTCAACAC CCCAAGGTTC GTTGAAGACA 
CTTAGAAGAC CTCATGCAAG TAGCGTGAGC ACCGTGAAGT CTGTGGCCCA AAGTCTTAAG 
TCGGATATTC CATTATTCGT TCAACCGGAG GATTTTGGCA CTATCCAGAT TGAAGTACTG 
AGCACATTGT ACAGGGATAA TGAAGATGAT TTATCCATTT TAATTGCAAT CATAGACAGG 
AAATCCGGTA AGGAGATGTT TAAATTTTCA AAATCTATTC ATAAAGTTCG CGAATTAGAT 
GTATATATGA AATCACATGT TCCCGATTTA CCATTGCCCA CGTTACCTGA TAGACAATTA 
TTTCAGACTT TATCACCTAC AAAAGTGGAT ACGCGAAAGA ACATATTGAA TCAGTATTAT 
ACAAGCATAT TCAGTGTCCC TGAATTTCCC AAAAATGTTG GTTTAAAAAT CGCCCAATTT 
ATTAGTACAG ATACAGTAAT GACACCTCCA ATGATGGATG ATAATGTGAA AGACGGATCA 
CTTTTGCTGA GAAGACCAAA GACTCTAACA GGTAATAGTA CATGGAGAGT TCGGTACGGT 
ATTTTGCGCG ATGATGTTTT ACAATTGTTT GACAAAAATC AGTTGACTGA AACTATTAAA 
TTGAGACAAT CTTCTATAGA ACTTATACCC AATCTCCCTG AAGATAGATT TGGAACGAGA 
AATGGGTTTT TGATCACTGA GCATAAGAAA AGCGGACTGT CAACCTCAAC AAAGTATTAT 
ATTTGTACTG AAACGTCGAA GGAACGTGAA TTATGGCTTT CTGCGTTTAG TGATTACATT 
GATCCTTCAC AAAGTTTATC GTTATCTAGC TCGAGGAATG CAAACGATAC TGATTCTGCA 
TCTCACTTGA GCGCAGGAAC ACACCATTCT AAATTTGGAA ACGCAACTAT CTCAGCAACC 
GATACTCCAT CATATGTCAC TGATTTGACC CAAGAATATA ATAATAATAA CAATATTAGT 
AATAGTAGCA ATAACATTGC TAATAGCGAT GGTATTGACT CAAACCCATC TTCTCACTCT 
AACTTCCTAG CAAGTTCAAG CGGGAATGCA GAGGAGGAGA AGGATAGTAG GAGAGCCAAA 
ATGAGAAGTT TATTCCCTTT TAAAAAATTA ACCGGCCCGG CGAGTGCAAT GAACCATATA 
GGTATTACAA TTTCAAATGA TTCGGATTCT CCAACATCTC CTGACTCTAT CATTAAGTCA 
CCAAGCAAAA AATTAATGGA AGTATCCTCT TCGTCAAATT CTTCTACGGG TCCACATGTG 
TCCACTGCAA TATTTGGCTC GTCACTAGAA ACGTGTTTAA GATTGAGTTC GCATAAGTAC 
CAAAACGTTT ACGATTTACC GAGTGTAGTT TATCGTTGCT TAGAGTATCT TTACAAAAAC 
CGTGGTATCC AGGAAGAAGG CATATTTAGA CTAAGCGGGT CCAGTACAGT TATCAAAACT 
TTACAAGAAA GGTTTGATAA AGAGTACGAT GTAGATTTAT GTCGGTACAA TGAAAGTATT 
GAAGCTAAAG ATGATGAGGC ATCTCCAAGT CTTTACATTG GTGTGAATAC TGTAAGTGGG 
TTACTAAAGT TGTACTTGAG AAAGTTGCCC CATTTATTAT TTGGTGACGA ACAATTTTTG 
TCGTTTAAAA GAGTAGTTGA TGAAAATCAT AATAATCCTG TTCAAATATC ATTGGGGTTT 
AAGGAACTTA TTGAATCTGG CCTAGTTCCT CACGCTAACC TATCGTTGAT GTATGCTCTT 
TTTGAACTGC TGGTAAGGAT CAATGAGAAT AGTAAGTTTA ATAAGATGAA TCTAAGAAAT 
TTATGCATCG TGTTTTCCCC AACTCTAAAT ATTCCAATTA GTATGCTTCA ACCATTCATC 
ACTGACTTTG CGTGTATATT TCAGGGTGGT GAACCAGTTA AAGAAGAGGA AAGGGAGAAA 
GTAGATATAC ATATTCCTCA GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BEM3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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