ORF » Species Summary » Saccharomyces cerevisiae S288C » HEL1 cDNA ORF clone

HEL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following HEL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03074 NM_001179807.3
Latest version!		 Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase HEL1 (HEL1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    HEL1 ( NM_001179807.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012942.3 Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase HEL1 (HEL1), partial mRNA.
    Price & Availability↑
    CloneID OSi03074
    Clone ID Related Accession (Same CDS sequence) NM_001179807.3
    Accession Version NM_001179807.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product E3 ubiquitin-protein ligase HEL1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179807.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGTAGTG GTACCGAAAA TGATCAATTT TATAGTTTTG ATGAAAGTGA TTCCAGCTCT 
    ATTGAACTTT ATGAATCTCA CAATACATCT GAATTTACTA TACATGGACT AGTTTTCCCG 
    AAGTTAATTA GTGTAACAAG CCAAGATTCA GAATTTGACA TCAATGAAGA TGAAGATGGA 
    GTCGATACCA TATATGAGGG GATGCTAGAT GCTCCTCTTA CGAAAAACAA CAAAAGAATA 
    CTGTGTGAAG GGAGTGTACC GAATCTCAGT TATGAATGCT TAACCACCAA GGGTATATTT 
    GAAAGAATGC TTCAAAGGGT TGACCATTTA CAACCTATTT TTGCAATTCC ATCTGCGGAT 
    ATATTGATAT TACTACAGCA TTATGATTGG AACGAGGAAC GTTTGCTAGA AGTTTGGACC 
    GAAAAAATGG ACGAATTGCT TGTGGAGCTT GGCTTAAGTA CCACAGCAAA TATCAAGAAA 
    GATAATGATT ATAATTCACA TTTCAGAGAA GTTGAATTCA AAAATGACTT TACATGTATT 
    ATTTGTTGTG ATAAAAAGGA TACGGAAACA TTTGCGTTGG AATGTGGCCA TGAATATTGT 
    ATCAATTGCT ACCGTCATTA CATCAAGGAT AAGTTGCATG AGGGGAACAT CATTACATGC 
    ATGGACTGTT CCCTGGCATT GAAAAATGAA GATATAGATA AAGTGATGGG ACATCCTTCG 
    AGTAGTAAGT TGATGGATTC TTCAATCAAA AGTTTTGTTC AAAAACATAA TCGTAACTAT 
    AAATGGTGCC CGTTTGCTGA CTGTAAATCC ATCGTGCACT TGCGAGATAC GTCATCATTA 
    CCAGAATATA CCCGTTTGCA TTATTCACCT TTCGTTAAGT GTAATTCATT TCATAGATTC 
    TGTTTCAATT GCGGATTCGA AGTTCATTCC CCAGCAGATT GTAAAATAAC CACTGCTTGG 
    GTTAAAAAAG CCAGAAAAGA ATCAGAAATT TTGAATTGGG TCTTGTCCCA CACTAAAGAA 
    TGTCCAAAAT GTTCTGTTAA TATTGAAAAA AATGGTGGCT GCAACCACAT GGTTTGCTCA 
    AGTTGTAAAT ACGAATTCTG TTGGATTTGT GAGGGCCCCT GGGCGCCCCA CGGTAAGAAC 
    TTTTTTCAAT GTACAATGTA TAAGAATAAT GAGGATAATA AAAGTAAGAA CCCTCAAGAC 
    GCTAATAAAA CCTTGAAGAA ATACACTTTC TACTATAGAT TGTTTAACGA ACATGAAGTT 
    TCAGCCAAGC TTGATTGGAA CCTGGGGCAA ACTTTGGGCA CGAAAGTTCA TGCACTACAA 
    GAAAGAATAG GTATCTCATG GATTGATGGA CAGTTCCTAT CAGAAAGTTT AAAAGTGTTG 
    AATGAGGGAC GAACCGTATT AAAATGGTCA TTTGCCGTGG CATATTACTC GGATGCATCT 
    CATAATTTGA CGAAGATTTT CGTTGATAAC CAAATGCTCT TAGCCAACGC AGTGGAATCG 
    CTATCGGAGC TATTGCAAAT AAAAACCCCC GAAGTGATCA TGAAACGAAG ACCAGAGTTT 
    TATAATAAGG CCGGATATGT GGAAAATAGA ACCACTGCAT TAATGGAATG CGGTAGAGAA 
    CTGCTATGTA AGGGAATTTG CAAAGCTGCA GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_012942.3
    CDS1..1656
    Translation

    Target ORF information:

    RefSeq Version NM_001179807.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase HEL1 (HEL1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001179807.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGTAGTG GTACCGAAAA TGATCAATTT TATAGTTTTG ATGAAAGTGA TTCCAGCTCT 
    ATTGAACTTT ATGAATCTCA CAATACATCT GAATTTACTA TACATGGACT AGTTTTCCCG 
    AAGTTAATTA GTGTAACAAG CCAAGATTCA GAATTTGACA TCAATGAAGA TGAAGATGGA 
    GTCGATACCA TATATGAGGG GATGCTAGAT GCTCCTCTTA CGAAAAACAA CAAAAGAATA 
    CTGTGTGAAG GGAGTGTACC GAATCTCAGT TATGAATGCT TAACCACCAA GGGTATATTT 
    GAAAGAATGC TTCAAAGGGT TGACCATTTA CAACCTATTT TTGCAATTCC ATCTGCGGAT 
    ATATTGATAT TACTACAGCA TTATGATTGG AACGAGGAAC GTTTGCTAGA AGTTTGGACC 
    GAAAAAATGG ACGAATTGCT TGTGGAGCTT GGCTTAAGTA CCACAGCAAA TATCAAGAAA 
    GATAATGATT ATAATTCACA TTTCAGAGAA GTTGAATTCA AAAATGACTT TACATGTATT 
    ATTTGTTGTG ATAAAAAGGA TACGGAAACA TTTGCGTTGG AATGTGGCCA TGAATATTGT 
    ATCAATTGCT ACCGTCATTA CATCAAGGAT AAGTTGCATG AGGGGAACAT CATTACATGC 
    ATGGACTGTT CCCTGGCATT GAAAAATGAA GATATAGATA AAGTGATGGG ACATCCTTCG 
    AGTAGTAAGT TGATGGATTC TTCAATCAAA AGTTTTGTTC AAAAACATAA TCGTAACTAT 
    AAATGGTGCC CGTTTGCTGA CTGTAAATCC ATCGTGCACT TGCGAGATAC GTCATCATTA 
    CCAGAATATA CCCGTTTGCA TTATTCACCT TTCGTTAAGT GTAATTCATT TCATAGATTC 
    TGTTTCAATT GCGGATTCGA AGTTCATTCC CCAGCAGATT GTAAAATAAC CACTGCTTGG 
    GTTAAAAAAG CCAGAAAAGA ATCAGAAATT TTGAATTGGG TCTTGTCCCA CACTAAAGAA 
    TGTCCAAAAT GTTCTGTTAA TATTGAAAAA AATGGTGGCT GCAACCACAT GGTTTGCTCA 
    AGTTGTAAAT ACGAATTCTG TTGGATTTGT GAGGGCCCCT GGGCGCCCCA CGGTAAGAAC 
    TTTTTTCAAT GTACAATGTA TAAGAATAAT GAGGATAATA AAAGTAAGAA CCCTCAAGAC 
    GCTAATAAAA CCTTGAAGAA ATACACTTTC TACTATAGAT TGTTTAACGA ACATGAAGTT 
    TCAGCCAAGC TTGATTGGAA CCTGGGGCAA ACTTTGGGCA CGAAAGTTCA TGCACTACAA 
    GAAAGAATAG GTATCTCATG GATTGATGGA CAGTTCCTAT CAGAAAGTTT AAAAGTGTTG 
    AATGAGGGAC GAACCGTATT AAAATGGTCA TTTGCCGTGG CATATTACTC GGATGCATCT 
    CATAATTTGA CGAAGATTTT CGTTGATAAC CAAATGCTCT TAGCCAACGC AGTGGAATCG 
    CTATCGGAGC TATTGCAAAT AAAAACCCCC GAAGTGATCA TGAAACGAAG ACCAGAGTTT 
    TATAATAAGG CCGGATATGT GGAAAATAGA ACCACTGCAT TAATGGAATG CGGTAGAGAA 
    CTGCTATGTA AGGGAATTTG CAAAGCTGCA GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome XI.
    Nature369(6479)371-8(1994 Jun)
    Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW


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