HSP12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HSP12
Entrez Gene ID	850532
Full Name	lipid-binding protein HSP12
Synonyms	GLP1,HOR5
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179952.1	NP_116640.1	lipid-binding protein HSP12

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	HSP12	NP_116640.1
Kluyveromyces lactis	KLLA0D07634g	XP_453403.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results demonstrate that the combined action of Hsp12p, Pau5p and a killer toxin is sufficient to steer a yeast community.
Title: Hsp12p and PAU genes are involved in ecological interactions between natural yeast strains.

Hsp12 expression contributes to lifespan extension, possibly via membrane alterations
Title: NMR structure of Hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast.

NMR spectra collected in presence of paramagnetic spin relaxation agents (5DSA, 16DSA, and Gd(DTPA-BMA)) indicated that the amphipathic alpha-helices of Hsp12 in SDS micelles lie on the membrane surface.
Title: Structural characterization of Hsp12, the heat shock protein from Saccharomyces cerevisiae, in aqueous solution where it is intrinsically disordered and in detergent micelles where it is locally ?-helical.

Hsp12 is completely unfolded; however, in the presence of certain lipids, it adopts a helical structure. The presence of Hsp12 does not alter the overall lipid composition of the plasma membrane but increases membrane stability.
Title: Hsp12 is an intrinsically unstructured stress protein that folds upon membrane association and modulates membrane function.

The decrease of the turgor pressure in the case of WT strain upon addition of Congo red is shown to be consistent with an upregulation of Hsp12p in the close vicinity of the plasma membrane.
Title: The role of the heat shock protein Hsp12p in the dynamic response of Saccharomyces cerevisiae to the addition of Congo red.

The following HSP12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSP12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00221	NM_001179952.1 Latest version!	Saccharomyces cerevisiae S288C lipid-binding protein HSP12 (HSP12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSP12 ( NM_001179952.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116640.1 Saccharomyces cerevisiae S288C lipid-binding protein HSP12 (HSP12), partial mRNA.

Price & Availability↑

CloneID	OSi00221
Clone ID Related Accession (Same CDS sequence)	NM_001179952.1
Accession Version	NM_001179952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	lipid-binding protein HSP12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGACG CAGGTAGAAA AGGATTCGGT GAAAAAGCTT CTGAAGCTTT GAAGCCAGAC 
TCTCAAAAGT CATACGCTGA ACAAGGTAAG GAATACATCA CTGACAAGGC CGACAAGGTC 
GCTGGTAAGG TTCAACCAGA AGACAACAAG GGTGTCTTCC AAGGTGTCCA CGACTCTGCC 
GAAAAAGGCA AGGATAACGC TGAAGGTCAA GGTGAATCTT TGGCAGACCA AGCTAGAGAT 
TACATGGGAG CCGCCAAGTC CAAGTTGAAC GATGCCGTCG AATATGTTTC CGGTCGTGTC 
CACGGTGAAG AAGACCCAAC CAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116640.1
CDS	1..330
Translation	MSDAGRKGFGEKASEALKPDSQKSYAEQGKEYITDKADKVAGKVQPEDNKGVFQGVHDSAEKGKDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK

Target ORF information:

RefSeq Version	NM_001179952.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C lipid-binding protein HSP12 (HSP12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179952.1

ORF Insert Sequence:

ATGTCTGACG CAGGTAGAAA AGGATTCGGT GAAAAAGCTT CTGAAGCTTT GAAGCCAGAC 
TCTCAAAAGT CATACGCTGA ACAAGGTAAG GAATACATCA CTGACAAGGC CGACAAGGTC 
GCTGGTAAGG TTCAACCAGA AGACAACAAG GGTGTCTTCC AAGGTGTCCA CGACTCTGCC 
GAAAAAGGCA AGGATAACGC TGAAGGTCAA GGTGAATCTT TGGCAGACCA AGCTAGAGAT 
TACATGGGAG CCGCCAAGTC CAAGTTGAAC GATGCCGTCG AATATGTTTC CGGTCGTGTC 
CACGGTGAAG AAGACCCAAC CAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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HSP12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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