RIM15 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RIM15
Entrez Gene ID	850511
Full Name	protein kinase RIM15
Synonyms	TAK1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179933.1	NP_116620.1	protein kinase RIM15

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACR218W	NP_983620.2
Saccharomyces cerevisiae (baker's yeast)	RIM15	NP_116620.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rim15, a Greatwall-family protein kinase in Saccharomyces cerevisiae, is required for cellular nutrient responses, such as the entry into quiescence and the induction of meiosis and sporulation. (Review)
Title: Pleiotropic functions of the yeast Greatwall-family protein kinase Rim15p: a novel target for the control of alcoholic fermentation.

Validity of promoter engineering of the RIM15 gene that governs inhibitory control of alcoholic fermentation.
Title: Promoter engineering of the Saccharomyces cerevisiae RIM15 gene for improvement of alcoholic fermentation rates under stress conditions.

Rim15 and Sch9 kinases are involved in induction of autophagic degradation of ribosomes in budding yeast
Title: Rim15 and Sch9 kinases are involved in induction of autophagic degradation of ribosomes in budding yeast.

ur data support the hypothesis that metabolic reprogramming to accumulate energy stores and the activation of anti-oxidant defence systems are coordinated by Yak1, Rim15 and Mck1 kinases to ensure quiescence entry and lifespan extension in yeast.
Title: Chronological Lifespan in Yeast Is Dependent on the Accumulation of Storage Carbohydrates Mediated by Yak1, Mck1 and Rim15 Kinases.

G0/G1 transition, via Rim15 and CLN3, might govern the fermentation rate of bottom-fermenting brewer's yeast in high-gravity wort.
Title: Defective quiescence entry promotes the fermentation performance of bottom-fermenting brewer's yeast.

The following RIM15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIM15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00204	NM_001179933.1 Latest version!	Saccharomyces cerevisiae S288C protein kinase RIM15 (RIM15), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIM15 ( NM_001179933.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116620.1 Saccharomyces cerevisiae S288C protein kinase RIM15 (RIM15), partial mRNA.

Price & Availability↑

CloneID	OSi00204
Clone ID Related Accession (Same CDS sequence)	NM_001179933.1
Accession Version	NM_001179933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase RIM15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTTCAATA GAAGTAACAC CGCAGGCGGA TCTCAGGCTA TGAAAGAGGG ACTTGGCATA 
AACAAGCTCT CCCCGATATC ATCGAATTCG AACCCAAGCT CATTGACTTC CTCCAATTAT 
GAAAAATATC TGCAGCTGGC CACAGAGAAG AATCCGTGTA TGATCTTGGA GCTGGAACTG 
GACGGCAAGG TGCGATATGG CTCTCCACAG TGGAACACGA TCACAGGAGT CGCCGATGAT 
AGTGGCTCTT CTCCGACGTA CATTGCAGAC CTTATTCTCG GATCCGATCA AGATAAAGGT 
GTCTTTCAAA AGGCCACAGA CATGCTGCTC ATGAATGATG ACACCAGTTG CACTATAACG 
TTCAAGATAA AGGCAGCCGA CTATGAAGGT AGCGCAGGCT GTGACGATGA AAGTACGATA 
ACGACCTTGG AAGCACGTGG TATCTTAATC AGGGATGGCC ACACACAGTT GCCCTCTCAC 
ACGATGTGGA TAGTCAAGCC TCGCACAAAC GACTGGTCAG ACTTTTATGC CAACGAAGAC 
GCTCAAGACG ACATGGTCAT CCAGTTATCC GATAATTGCG ACGATATCGA TATCCAACTT 
CCCGAAGAGT TCGCCAAGAC GCTTGGGTTC GGCGCTAAGA TCTTCGTGCA GTACTTGAAG 
AGAATACGAC TGGAAATGAT AATAGACGAG TTCAATCTAC CTCTGCCAAA AATGGAACTA 
TGCCGGGTCT GTGAGAACTT TGTCCCTGTT TGGTGGTTGG AGACCCATTC GCAAAGTTGC 
GTTTGCGAGC ATAGAACGGA ATCGCTCATA CAATTACTAC ACGATAATCT TCTTGAGCAA 
CAGGCGATCT TGGCAAACTT CACGAAAGAT TCAGAGTATA AGGGCAGTCA GATACAGGTA 
CGTTCCAACA ACTTCCTTAA CCAAGTTTTA GACTCCTTAA GAGAGCTGTG TCAGGACGCC 
ATAGATATCA ACCCGAGTGA AATGGTTCCT GATCTTTACC ACAGTCTTTC AACATTTCCT 
CAAGATAATG GTAATAATAA CAATAATAAT AATAATAATA ATAATAATAA CAATGCTTTG 
TTAGATCAAT TCCCTATCCA AAAAGATACA GTTAGCTTGA ATTCATATTT TCAGTTTTCC 
CCAAGGACTA ACCACAACAT TCAAAACGTC ACGTCGTGGC AATCAAGATT TTTTCTCAAT 
GATGATCAGG ATCCTGGACT AGCTCTTTTG ATTCACGATA CTCTGGACTT GGCAAGGAAA 
AAAGTGGATG CCGTGTTGAG GTTGGATAAC GCAATGACCT ATTCTTTAAA GATTAAAAAC 
GAGGTCAACA ACTATGTGGT ACAACTGATC CGCGAGCAAA TTGAAATAAA TAAGCATGCA 
ATCCTAACTC ACCCAATGAA TTTAAGGTCT TCTTCCATAT TTCATTCCCC ACTGCCGCAA 
ATTCACTCTC AACAACCAGA AGCCGAGAAT CTCATATATT CCTCCTCTAC TCCCCTGCAA 
GTCCAACACG ACCAATGTGC GTCCTTTGAA GCACCCTCCA AGTCTCATCT GGAGCCTATT 
CCTTTCCCGG TTTCTTCCAT TGAAGAAACA CCAACTGCAA ATGATATCAG GCATCCTTCT 
CCTTTGCCCC GTAGTTGTAG CAACACCGTT ATGAAACTAC CGACACCTCG AAGGAAACTT 
GACTCAAACG GATTATTCTC TGATGCCTAT TTAAACGCTG ACATCATTCC GAACCCAAGT 
ATCGAATCCA CGATATCTAT TGATAGAGAT AATAACACTA ATAGTAGGGG TAGTAGTATG 
AAACAGTATG GTATTGGTGA AGCCACCGAC TCTCGGACTA GTAACTCGGA AAGACCTTCT 
TCCTCTTCGT CAAGGCTGGG GATAAGATCA AGATCCATAA CACCAAGACA AAAGATAGAA 
TACTCACATG TAGATAATGA TGACCGCACC AACGAAATGC TGTCTAGAGA TAAAGATTCT 
CTTCAACCTC AACCTTCCGT AGATACCACC ATAACATCCT CTACTCAGGC GACCACCACG 
GGTACCAAGA CTAATAGTAA CAATTCCACA AACTCAGTAT TACCAAAACT AATGACAAGT 
ATTTCCTTGA CCCCAAGGCG TGGTTCACCA TCATTTGGTA ATCTCGCAAG CCATTCTATG 
CAGCAGACAA ACAGTTTTAA ACTGATTCAT GATAAATCGC CGATATCTTC ACCTTTCACA 
TTCTCCAAGG ATTTTTTAAC CCCAGAGCAG CACCCTTCCA ATATTGCCAG AACAGATAGT 
ATCAATAATG CAATGTTAAC TTCACCGAAT ATGCCATTAT CACCCCTTTT ATTGGCCACA 
AACCAAACTG TTAAATCTCC AACGCCTAGC ATAAAAGATT ACGATATCTT GAAACCAATC 
AGCAAAGGTG CTTATGGTAG TGTTTATCTA GCACGGAAAA AACTCACAGG AGATTATTTT 
GCTATAAAGG TTCTAAGGAA ATCAGATATG ATTGCCAAAA ATCAAGTAAC AAATGTCAAA 
TCCGAGAGAG CAATCATGAT GGTTCAAAGT GATAAGCCCT ATGTTGCGAG ACTATTTGCT 
AGTTTCCAAA ATAAAGATAA CCTTTTCTTA GTGATGGAAT ATTTACCAGG TGGAGATTTG 
GCCACTTTAA TCAAGATGAT GGGGTATCTG CCCGATCAAT GGGCCAAGCA ATACCTAACC 
GAAATCGTTG TCGGTGTGAA TGATATGCAT CAAAATGGGA TCATTCATCA TGACTTAAAG 
CCTGAAAATC TACTAATTGA TAATGCAGGT CATGTGAAAT TAACAGATTT CGGTTTATCA 
AGAGCTGGTC TGATTCGCCG TCACAAGTTT GTCCCACATA AGTCGTCGCT AAGTATCAGT 
TCCACTTTAC CAATCGATAA CCCAGCAAAT AATTTTACCA TGAACAACAA CAATAGTAAT 
CATTCTCAAT TATCAACCCC AGATAGCTTC ACATCAGATC ATAAGCAGTA TAATAGAAGC 
AAGAAGTCAT CACTAGGTCA GCAATACGAA CACTCAGAAT ACTCAAGTAC TTCCAATTCC 
CACTCAATGA CGCCAACGCC CAGTACGAAC ACTGTTGTTT ATCCTTCATA TTACCGTGGG 
AAGGACAGAT CACACGGAAG TTCGAACATC GATCTCCCAG CGTCCCTTAG AAGAAGTGAA 
TCTCAATTAT CATTTTCCCT CCTTGATATT TCTCGTTCTA GTACTCCTCC TTTAGCAAAT 
CCCACAAATT CGAACGCTAA TAATATTATG AGAAGGAAAT CACTCACTGA GAATAAATCC 
TTTTCTAATG ACCTATTATC TTCAGATGCT ATCGCAGCTA CCAATACGAA TATTAACTCG 
AATAATAACA TTTCCCTTTC GCCAGCACCT TCGGATTTAG CTTTGTTTTA TCCTGATGAT 
AGCAAGCAAA ATAAGAAATT TTTTGGGACT CCCGATTATC TCGCTCCAGA AACTATTGAA 
GGAAAGGGTG AAGATAACAA GCAATGCGAC TGGTGGTCAG TTGGTTGTAT ATTTTTCGAA 
TTACTTTTAG GGTATCCTCC ATTCCATGCA GAAACACCAG ATGCTGTTTT TAAGAAAATT 
CTATCAGGAG TCATTCAATG GCCAGAGTTT AAAAATGAAG AAGAAGAGCG AGAATTCCTA 
ACACCAGAGG CAAAAGATTT GATAGAAAAA TTGTTGGTTG TGGATCCTGC GAAAAGACTG 
GGTGCGAAAG GAATTCAAGA AATTAAAGAT CACCCTTATT TCAAGAATGT GGATTGGGAT 
CATGTTTACG ATGAGGAAGC TTCTTTTGTC CCTACAATAG ACAATCCAGA AGATACTGAT 
TATTTTGACC TAAGGGGTGC AGAGCTCCAA GATTTTGGAG ACGATATCGA AAACGATAAT 
GCCAATATTT TGTTTGGTAA ACATGGCATT AACACCGATG TTTCTGAATT ATCTGCAGCT 
AATCTCTCTC CACCATTGAA TCATAAAAAT ATTTTATCCC GTAAACTATC GATGAGTAAC 
ACCACTAATA GGAGCTCAAA TAATTCCAAC AGTAGCGTGC ATGACTTTGG TGCACATACA 
CCGGTTAATA AATTAAGTAT TGCTTCTGTA TTAGAGTCAG TACCTCAAGA AACAGGATAT 
ATTACACCTA ACGGGACCGG TACAACTACT ACAAGTGCCA AAAACTCACC CAATCTGAAG 
AATTTGTCAC TGGCTATACC TCCACATATG AGGGATCGCA GATCAAGTAA ATTGAATGAT 
TCACAAACGG AATTTGGTTC TTTTAATTTC AGGAATTTAT CGGCTCTTGA TAAAGCTAAT 
AAAGATGCTA TAAATAGACT GAAAAGTGAA CATTTTTCTG AACAACCTGG GGTTCACAGA 
AGAACCTCTT CTGCGTCACT AATGGGGTCA TCCTCAGACG GATCAGTGTC AACTCCAGGG 
AGTAACGCTT CAAACACTAC ATCTGGTGGC AAGTTGAAAA TACATAAGCC TACCATATCC 
GGTTCTCCTT CAACATTTGG CACATTTCCC AAAACATTTT TGAGGTCTGA TTCATTCTCC 
ACAAGATCAT ATTCTCCTGA ACGAAGTATT AGTATCGACT CGTCAACATT ATCAAGGAAG 
GGTAGTATAA TCGGGGATAA CCAACAAACA ACAGCAAATA GCTCGGATTC ACCTACGATG 
ACTAAATTCA AGTCGCCACT ATCACCTGCT AATACCACCA CCGTGAGCTC ATATTTTTCA 
AGACAGAGGG TTCTATCAAA GAGTTTTTCG CAACGGACCA ATTCCAGTGA TCTCTCGGCA 
GAGGAAAGCG ACCGACTACA GGCTATATCA AGAGTTAACT CTTTAAGAAA CAGGAGGCGT 
AGTGGCCGAA AGAGCTCGAG CACTTCTGAG ATTGGATACC ACATGGATGT TCTTGTTTGT 
GAGCCTATAC CGATTCATAG ATATCGGGTT ACTAAAGACT TAGAAAATTT GGGCTGTACC 
GTCGTCAGTG TTGGTGCCGG TGATGAACTA GTTAGTAGAG CCACTAGTGG TGTAAGTTTT 
GACTTAATTA TGACAGCCTT GAAGCTTCCA AAACTTGGTG CTATTGACAT TGTTCAACTA 
CTAAAGCAAA CAAATGGTGC TAATTCGACA ACACCAATTG TGGCCATAAC AAATTATTTT 
CAGGAGGCGG CAACCAGTAG AGTCTTTGAC GATGTTTTAG AAAAACCGGT AAAACTTGAC 
GAGCTAAAAA AATTGGTGGC TAAGTACGCA CTGAAAAAGT CTCAAGAAGA TGAAGAGCAT 
ACTATATTGA GCGATTCTGA TGAAACGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116620.1
CDS	1..5313
Translation	MFNRSNTAGGSQAMKEGLGINKLSPISSNSNPSSLTSSNYEKYLQLATEKNPCMILELELDGKVRYGSPQWNTITGVADDSGSSPTYIADLILGSDQDKGVFQKATDMLLMNDDTSCTITFKIKAADYEGSAGCDDESTITTLEARGILIRDGHTQLPSHTMWIVKPRTNDWSDFYANEDAQDDMVIQLSDNCDDIDIQLPEEFAKTLGFGAKIFVQYLKRIRLEMIIDEFNLPLPKMELCRVCENFVPVWWLETHSQSCVCEHRTESLIQLLHDNLLEQQAILANFTKDSEYKGSQIQVRSNNFLNQVLDSLRELCQDAIDINPSEMVPDLYHSLSTFPQDNGNNNNNNNNNNNNNNALLDQFPIQKDTVSLNSYFQFSPRTNHNIQNVTSWQSRFFLNDDQDPGLALLIHDTLDLARKKVDAVLRLDNAMTYSLKIKNEVNNYVVQLIREQIEINKHAILTHPMNLRSSSIFHSPLPQIHSQQPEAENLIYSSSTPLQVQHDQCASFEAPSKSHLEPIPFPVSSIEETPTANDIRHPSPLPRSCSNTVMKLPTPRRKLDSNGLFSDAYLNADIIPNPSIESTISIDRDNNTNSRGSSMKQYGIGEATDSRTSNSERPSSSSSRLGIRSRSITPRQKIEYSHVDNDDRTNEMLSRDKDSLQPQPSVDTTITSSTQATTTGTKTNSNNSTNSVLPKLMTSISLTPRRGSPSFGNLASHSMQQTNSFKLIHDKSPISSPFTFSKDFLTPEQHPSNIARTDSINNAMLTSPNMPLSPLLLATNQTVKSPTPSIKDYDILKPISKGAYGSVYLARKKLTGDYFAIKVLRKSDMIAKNQVTNVKSERAIMMVQSDKPYVARLFASFQNKDNLFLVMEYLPGGDLATLIKMMGYLPDQWAKQYLTEIVVGVNDMHQNGIIHHDLKPENLLIDNAGHVKLTDFGLSRAGLIRRHKFVPHKSSLSISSTLPIDNPANNFTMNNNNSNHSQLSTPDSFTSDHKQYNRSKKSSLGQQYEHSEYSSTSNSHSMTPTPSTNTVVYPSYYRGKDRSHGSSNIDLPASLRRSESQLSFSLLDISRSSTPPLANPTNSNANNIMRRKSLTENKSFSNDLLSSDAIAATNTNINSNNNISLSPAPSDLALFYPDDSKQNKKFFGTPDYLAPETIEGKGEDNKQCDWWSVGCIFFELLLGYPPFHAETPDAVFKKILSGVIQWPEFKNEEEEREFLTPEAKDLIEKLLVVDPAKRLGAKGIQEIKDHPYFKNVDWDHVYDEEASFVPTIDNPEDTDYFDLRGAELQDFGDDIENDNANILFGKHGINTDVSELSAANLSPPLNHKNILSRKLSMSNTTNRSSNNSNSSVHDFGAHTPVNKLSIASVLESVPQETGYITPNGTGTTTTSAKNSPNLKNLSLAIPPHMRDRRSSKLNDSQTEFGSFNFRNLSALDKANKDAINRLKSEHFSEQPGVHRRTSSASLMGSSSDGSVSTPGSNASNTTSGGKLKIHKPTISGSPSTFGTFPKTFLRSDSFSTRSYSPERSISIDSSTLSRKGSIIGDNQQTTANSSDSPTMTKFKSPLSPANTTTVSSYFSRQRVLSKSFSQRTNSSDLSAEESDRLQAISRVNSLRNRRRSGRKSSSTSEIGYHMDVLVCEPIPIHRYRVTKDLENLGCTVVSVGAGDELVSRATSGVSFDLIMTALKLPKLGAIDIVQLLKQTNGANSTTPIVAITNYFQEAATSRVFDDVLEKPVKLDELKKLVAKYALKKSQEDEEHTILSDSDETH

Target ORF information:

RefSeq Version	NM_001179933.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase RIM15 (RIM15), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179933.1

ORF Insert Sequence:

ATGTTCAATA GAAGTAACAC CGCAGGCGGA TCTCAGGCTA TGAAAGAGGG ACTTGGCATA 
AACAAGCTCT CCCCGATATC ATCGAATTCG AACCCAAGCT CATTGACTTC CTCCAATTAT 
GAAAAATATC TGCAGCTGGC CACAGAGAAG AATCCGTGTA TGATCTTGGA GCTGGAACTG 
GACGGCAAGG TGCGATATGG CTCTCCACAG TGGAACACGA TCACAGGAGT CGCCGATGAT 
AGTGGCTCTT CTCCGACGTA CATTGCAGAC CTTATTCTCG GATCCGATCA AGATAAAGGT 
GTCTTTCAAA AGGCCACAGA CATGCTGCTC ATGAATGATG ACACCAGTTG CACTATAACG 
TTCAAGATAA AGGCAGCCGA CTATGAAGGT AGCGCAGGCT GTGACGATGA AAGTACGATA 
ACGACCTTGG AAGCACGTGG TATCTTAATC AGGGATGGCC ACACACAGTT GCCCTCTCAC 
ACGATGTGGA TAGTCAAGCC TCGCACAAAC GACTGGTCAG ACTTTTATGC CAACGAAGAC 
GCTCAAGACG ACATGGTCAT CCAGTTATCC GATAATTGCG ACGATATCGA TATCCAACTT 
CCCGAAGAGT TCGCCAAGAC GCTTGGGTTC GGCGCTAAGA TCTTCGTGCA GTACTTGAAG 
AGAATACGAC TGGAAATGAT AATAGACGAG TTCAATCTAC CTCTGCCAAA AATGGAACTA 
TGCCGGGTCT GTGAGAACTT TGTCCCTGTT TGGTGGTTGG AGACCCATTC GCAAAGTTGC 
GTTTGCGAGC ATAGAACGGA ATCGCTCATA CAATTACTAC ACGATAATCT TCTTGAGCAA 
CAGGCGATCT TGGCAAACTT CACGAAAGAT TCAGAGTATA AGGGCAGTCA GATACAGGTA 
CGTTCCAACA ACTTCCTTAA CCAAGTTTTA GACTCCTTAA GAGAGCTGTG TCAGGACGCC 
ATAGATATCA ACCCGAGTGA AATGGTTCCT GATCTTTACC ACAGTCTTTC AACATTTCCT 
CAAGATAATG GTAATAATAA CAATAATAAT AATAATAATA ATAATAATAA CAATGCTTTG 
TTAGATCAAT TCCCTATCCA AAAAGATACA GTTAGCTTGA ATTCATATTT TCAGTTTTCC 
CCAAGGACTA ACCACAACAT TCAAAACGTC ACGTCGTGGC AATCAAGATT TTTTCTCAAT 
GATGATCAGG ATCCTGGACT AGCTCTTTTG ATTCACGATA CTCTGGACTT GGCAAGGAAA 
AAAGTGGATG CCGTGTTGAG GTTGGATAAC GCAATGACCT ATTCTTTAAA GATTAAAAAC 
GAGGTCAACA ACTATGTGGT ACAACTGATC CGCGAGCAAA TTGAAATAAA TAAGCATGCA 
ATCCTAACTC ACCCAATGAA TTTAAGGTCT TCTTCCATAT TTCATTCCCC ACTGCCGCAA 
ATTCACTCTC AACAACCAGA AGCCGAGAAT CTCATATATT CCTCCTCTAC TCCCCTGCAA 
GTCCAACACG ACCAATGTGC GTCCTTTGAA GCACCCTCCA AGTCTCATCT GGAGCCTATT 
CCTTTCCCGG TTTCTTCCAT TGAAGAAACA CCAACTGCAA ATGATATCAG GCATCCTTCT 
CCTTTGCCCC GTAGTTGTAG CAACACCGTT ATGAAACTAC CGACACCTCG AAGGAAACTT 
GACTCAAACG GATTATTCTC TGATGCCTAT TTAAACGCTG ACATCATTCC GAACCCAAGT 
ATCGAATCCA CGATATCTAT TGATAGAGAT AATAACACTA ATAGTAGGGG TAGTAGTATG 
AAACAGTATG GTATTGGTGA AGCCACCGAC TCTCGGACTA GTAACTCGGA AAGACCTTCT 
TCCTCTTCGT CAAGGCTGGG GATAAGATCA AGATCCATAA CACCAAGACA AAAGATAGAA 
TACTCACATG TAGATAATGA TGACCGCACC AACGAAATGC TGTCTAGAGA TAAAGATTCT 
CTTCAACCTC AACCTTCCGT AGATACCACC ATAACATCCT CTACTCAGGC GACCACCACG 
GGTACCAAGA CTAATAGTAA CAATTCCACA AACTCAGTAT TACCAAAACT AATGACAAGT 
ATTTCCTTGA CCCCAAGGCG TGGTTCACCA TCATTTGGTA ATCTCGCAAG CCATTCTATG 
CAGCAGACAA ACAGTTTTAA ACTGATTCAT GATAAATCGC CGATATCTTC ACCTTTCACA 
TTCTCCAAGG ATTTTTTAAC CCCAGAGCAG CACCCTTCCA ATATTGCCAG AACAGATAGT 
ATCAATAATG CAATGTTAAC TTCACCGAAT ATGCCATTAT CACCCCTTTT ATTGGCCACA 
AACCAAACTG TTAAATCTCC AACGCCTAGC ATAAAAGATT ACGATATCTT GAAACCAATC 
AGCAAAGGTG CTTATGGTAG TGTTTATCTA GCACGGAAAA AACTCACAGG AGATTATTTT 
GCTATAAAGG TTCTAAGGAA ATCAGATATG ATTGCCAAAA ATCAAGTAAC AAATGTCAAA 
TCCGAGAGAG CAATCATGAT GGTTCAAAGT GATAAGCCCT ATGTTGCGAG ACTATTTGCT 
AGTTTCCAAA ATAAAGATAA CCTTTTCTTA GTGATGGAAT ATTTACCAGG TGGAGATTTG 
GCCACTTTAA TCAAGATGAT GGGGTATCTG CCCGATCAAT GGGCCAAGCA ATACCTAACC 
GAAATCGTTG TCGGTGTGAA TGATATGCAT CAAAATGGGA TCATTCATCA TGACTTAAAG 
CCTGAAAATC TACTAATTGA TAATGCAGGT CATGTGAAAT TAACAGATTT CGGTTTATCA 
AGAGCTGGTC TGATTCGCCG TCACAAGTTT GTCCCACATA AGTCGTCGCT AAGTATCAGT 
TCCACTTTAC CAATCGATAA CCCAGCAAAT AATTTTACCA TGAACAACAA CAATAGTAAT 
CATTCTCAAT TATCAACCCC AGATAGCTTC ACATCAGATC ATAAGCAGTA TAATAGAAGC 
AAGAAGTCAT CACTAGGTCA GCAATACGAA CACTCAGAAT ACTCAAGTAC TTCCAATTCC 
CACTCAATGA CGCCAACGCC CAGTACGAAC ACTGTTGTTT ATCCTTCATA TTACCGTGGG 
AAGGACAGAT CACACGGAAG TTCGAACATC GATCTCCCAG CGTCCCTTAG AAGAAGTGAA 
TCTCAATTAT CATTTTCCCT CCTTGATATT TCTCGTTCTA GTACTCCTCC TTTAGCAAAT 
CCCACAAATT CGAACGCTAA TAATATTATG AGAAGGAAAT CACTCACTGA GAATAAATCC 
TTTTCTAATG ACCTATTATC TTCAGATGCT ATCGCAGCTA CCAATACGAA TATTAACTCG 
AATAATAACA TTTCCCTTTC GCCAGCACCT TCGGATTTAG CTTTGTTTTA TCCTGATGAT 
AGCAAGCAAA ATAAGAAATT TTTTGGGACT CCCGATTATC TCGCTCCAGA AACTATTGAA 
GGAAAGGGTG AAGATAACAA GCAATGCGAC TGGTGGTCAG TTGGTTGTAT ATTTTTCGAA 
TTACTTTTAG GGTATCCTCC ATTCCATGCA GAAACACCAG ATGCTGTTTT TAAGAAAATT 
CTATCAGGAG TCATTCAATG GCCAGAGTTT AAAAATGAAG AAGAAGAGCG AGAATTCCTA 
ACACCAGAGG CAAAAGATTT GATAGAAAAA TTGTTGGTTG TGGATCCTGC GAAAAGACTG 
GGTGCGAAAG GAATTCAAGA AATTAAAGAT CACCCTTATT TCAAGAATGT GGATTGGGAT 
CATGTTTACG ATGAGGAAGC TTCTTTTGTC CCTACAATAG ACAATCCAGA AGATACTGAT 
TATTTTGACC TAAGGGGTGC AGAGCTCCAA GATTTTGGAG ACGATATCGA AAACGATAAT 
GCCAATATTT TGTTTGGTAA ACATGGCATT AACACCGATG TTTCTGAATT ATCTGCAGCT 
AATCTCTCTC CACCATTGAA TCATAAAAAT ATTTTATCCC GTAAACTATC GATGAGTAAC 
ACCACTAATA GGAGCTCAAA TAATTCCAAC AGTAGCGTGC ATGACTTTGG TGCACATACA 
CCGGTTAATA AATTAAGTAT TGCTTCTGTA TTAGAGTCAG TACCTCAAGA AACAGGATAT 
ATTACACCTA ACGGGACCGG TACAACTACT ACAAGTGCCA AAAACTCACC CAATCTGAAG 
AATTTGTCAC TGGCTATACC TCCACATATG AGGGATCGCA GATCAAGTAA ATTGAATGAT 
TCACAAACGG AATTTGGTTC TTTTAATTTC AGGAATTTAT CGGCTCTTGA TAAAGCTAAT 
AAAGATGCTA TAAATAGACT GAAAAGTGAA CATTTTTCTG AACAACCTGG GGTTCACAGA 
AGAACCTCTT CTGCGTCACT AATGGGGTCA TCCTCAGACG GATCAGTGTC AACTCCAGGG 
AGTAACGCTT CAAACACTAC ATCTGGTGGC AAGTTGAAAA TACATAAGCC TACCATATCC 
GGTTCTCCTT CAACATTTGG CACATTTCCC AAAACATTTT TGAGGTCTGA TTCATTCTCC 
ACAAGATCAT ATTCTCCTGA ACGAAGTATT AGTATCGACT CGTCAACATT ATCAAGGAAG 
GGTAGTATAA TCGGGGATAA CCAACAAACA ACAGCAAATA GCTCGGATTC ACCTACGATG 
ACTAAATTCA AGTCGCCACT ATCACCTGCT AATACCACCA CCGTGAGCTC ATATTTTTCA 
AGACAGAGGG TTCTATCAAA GAGTTTTTCG CAACGGACCA ATTCCAGTGA TCTCTCGGCA 
GAGGAAAGCG ACCGACTACA GGCTATATCA AGAGTTAACT CTTTAAGAAA CAGGAGGCGT 
AGTGGCCGAA AGAGCTCGAG CACTTCTGAG ATTGGATACC ACATGGATGT TCTTGTTTGT 
GAGCCTATAC CGATTCATAG ATATCGGGTT ACTAAAGACT TAGAAAATTT GGGCTGTACC 
GTCGTCAGTG TTGGTGCCGG TGATGAACTA GTTAGTAGAG CCACTAGTGG TGTAAGTTTT 
GACTTAATTA TGACAGCCTT GAAGCTTCCA AAACTTGGTG CTATTGACAT TGTTCAACTA 
CTAAAGCAAA CAAATGGTGC TAATTCGACA ACACCAATTG TGGCCATAAC AAATTATTTT 
CAGGAGGCGG CAACCAGTAG AGTCTTTGAC GATGTTTTAG AAAAACCGGT AAAACTTGAC 
GAGCTAAAAA AATTGGTGGC TAAGTACGCA CTGAAAAAGT CTCAAGAAGA TGAAGAGCAT 
ACTATATTGA GCGATTCTGA TGAAACGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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RIM15 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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