RAD3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD3
Entrez Gene ID	856918
Full Name	TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3
Synonyms	REM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179061.3	NP_011098.3	TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ercc2	NP_031975.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002988	XP_311900.4
Bos taurus (cattle)	ERCC2	NP_001096787.1
Magnaporthe oryzae (rice blast fungus)	MGG_03132	XP_003716866.1
Pan troglodytes (chimpanzee)	ERCC2	NP_001233519.1
Danio rerio (zebrafish)	ercc2	NP_957220.1
Drosophila melanogaster (fruit fly)	Xpd	NP_726036.2
Schizosaccharomyces pombe (fission yeast)	rad15	NP_593025.1
Oryza sativa (rice)	Os05g0144800	NP_001054627.1
Xenopus tropicalis (tropical clawed frog)	ercc2	NP_001008131.1
Homo sapiens (human)	ERCC2	NP_000391.1
Canis lupus familiaris (dog)	ERCC2	XP_541562.3
Kluyveromyces lactis	KLLA0C17776g	XP_452994.1
Neurospora crassa	NCU01625	XP_956536.2
Arabidopsis thaliana (thale cress)	UVH6	NP_171818.1
	LOC715485	XP_002808245.1
Rattus norvegicus (Norway rat)	Ercc2	NP_001166280.1
Caenorhabditis elegans (roundworm)	Y50D7A.2	NP_497182.2
Saccharomyces cerevisiae (baker's yeast)	RAD3	NP_011098.3
Eremothecium gossypii	AGOS_AGR114C	NP_986780.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both rad3-101 and rad3-102 elevate the frequency of reciprocal crossovers about 100-fold. Mapping of these events shows that three-quarters of these crossovers reflect DSBs formed at the same positions in both sister chromatids
Title: High-Resolution Mapping of Homologous Recombination Events in rad3 Hyper-Recombination Mutants in Yeast.

When the Rad3 ATP-binding groove is mutated, TFIIH gains a higher affinity for DNA.
Title: The rem mutations in the ATP-binding groove of the Rad3/XPD helicase lead to Xeroderma pigmentosum-Cockayne syndrome-like phenotypes.

In mms19 mutant cells, protein levels of the core TFIIH component Rad3 and Ssl2 were significantly reduced
Title: Mms19 protein functions in nucleotide excision repair by sustaining an adequate cellular concentration of the TFIIH component Rad3.

rad3-102, a mutant allele of the gene RAD3, may increase lesions that elicit a response by the recombination machinery or, alternatively, stabilize recombinagenic lesions generated by DNA replication failure
Title: A mutant allele of the transcription factor IIH helicase gene, RAD3, promotes loss of heterozygosity in response to a DNA replication defect in Saccharomyces cerevisiae.

The following RAD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05666	NM_001179061.3 Latest version!	Saccharomyces cerevisiae S288C TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3 (RAD3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD3 ( NM_001179061.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011098.3 Saccharomyces cerevisiae S288C TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3 (RAD3), partial mRNA.

Price & Availability↑

CloneID	OSi05666
Clone ID Related Accession (Same CDS sequence)	NM_001179061.3
Accession Version	NM_001179061.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001179061.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGTTTT ATATAGATGA TTTACCAGTG CTTTTTCCAT ACCCCAAGAT ATATCCAGAG 
CAGTATAATT ATATGTGCGA TATTAAAAAG ACTCTGGATG TAGGTGGAAA TAGTATCTTG 
GAGATGCCTT CAGGAACAGG TAAAACGGTC TCATTACTAT CCCTCACAAT TGCCTACCAG 
ATGCACTACC CAGAACATAG AAAGATCATA TATTGTTCTC GTACTATGTC TGAAATTGAA 
AAAGCTTTAG TAGAGTTAGA GAACCTTATG GATTACAGAA CTAAAGAACT AGGCTATCAA 
GAGGATTTTC GAGGTCTTGG CTTGACATCA AGAAAAAATT TGTGTTTGCA TCCCGAAGTG 
AGTAAGGAAC GAAAAGGTAC AGTAGTCGAT GAAAAGTGCC GTAGAATGAC AAATGGGCAG 
GCGAAGAGAA AATTAGAAGA GGATCCAGAG GCAAATGTAG AATTGTGTGA ATACCATGAG 
AATTTGTACA ATATTGAAGT AGAGGATTAT CTTCCAAAAG GCGTATTTTC TTTTGAAAAA 
CTTTTGAAAT ACTGCGAAGA AAAAACACTT TGTCCATATT TTATTGTTCG TCGTATGATT 
TCTCTTTGTA ACATTATTAT TTATTCTTAC CATTATCTAT TAGATCCTAA AATTGCTGAA 
AGAGTTTCCA ACGAGGTTTC TAAAGATAGC ATTGTCATTT TTGATGAAGC GCACAATATT 
GATAATGTGT GTATCGAATC GCTGTCATTA GACTTGACAA CGGATGCATT GAGAAGAGCC 
ACACGAGGTG CTAATGCGTT AGATGAACGT ATTTCTGAGG TCAGAAAGGT TGACTCACAG 
AAATTACAGG ATGAATACGA AAAACTAGTT CAAGGTCTCC ATTCTGCAGA TATTCTTACC 
GACCAGGAAG AGCCATTTGT GGAAACACCT GTATTGCCCC AAGATCTTCT AACAGAAGCA 
ATCCCGGGAA ATATACGAAG AGCCGAGCAT TTTGTTTCAT TTTTGAAAAG ATTGATAGAA 
TATCTGAAGA CCAGAATGAA AGTTCTTCAC GTTATTTCAG AAACGCCAAA ATCATTTCTA 
CAGCATTTAA AACAGTTAAC TTTCATAGAA AGGAAACCTC TTCGGTTTTG CTCAGAAAGG 
CTATCATTAC TTGTAAGAAC TTTAGAAGTT ACAGAGGTAG AAGATTTTAC TGCATTGAAA 
GACATAGCAA CTTTTGCTAC TCTTATATCA ACATATGAGG AAGGGTTTTT ACTAATTATT 
GAACCGTATG AAATCGAAAA TGCTGCAGTT CCGAATCCGA TTATGAGATT TACTTGCTTA 
GATGCATCGA TTGCCATCAA ACCAGTCTTT GAGAGATTTT CTTCCGTTAT TATCACTTCA 
GGGACCATAT CACCATTAGA CATGTATCCA AGAATGTTAA ACTTTAAAAC TGTTTTACAA 
AAATCATATG CCATGACCTT AGCCAAAAAA TCATTTCTAC CAATGATTAT TACCAAGGGT 
TCTGATCAAG TTGCAATTTC TTCAAGATTT GAAATCAGAA ACGATCCTAG TATTGTTCGT 
AATTACGGTT CTATGCTAGT AGAGTTTGCC AAGATCACAC CTGATGGAAT GGTTGTTTTT 
TTCCCCTCAT ATCTATATAT GGAAAGTATT GTTTCAATGT GGCAAACAAT GGGTATTCTT 
GACGAAGTTT GGAAACATAA ATTAATTTTA GTTGAGACTC CTGATGCTCA AGAAACTTCT 
TTAGCCTTAG AAACCTATAG AAAGGCTTGC TCAAATGGGC GTGGGGCAAT TTTGCTTTCT 
GTTGCTAGAG GAAAGGTATC TGAAGGTATC GATTTTGATC ATCAATATGG CAGAACTGTG 
CTGATGATAG GTATCCCGTT TCAATACACA GAATCGCGTA TTTTGAAAGC TCGCCTAGAA 
TTCATGAGGG AGAACTATCG CATCAGAGAA AACGACTTCT TATCTTTCGA TGCGATGAGA 
CATGCAGCTC AATGTCTGGG GAGAGTACTG AGAGGGAAGG ACGACTATGG TGTAATGGTA 
CTAGCAGACC GTAGGTTTTC AAGAAAAAGA AGCCAGTTAC CAAAATGGAT TGCTCAAGGT 
TTGTCTGACG CCGATTTGAA CCTTTCGACT GACATGGCCA TATCCAATAC CAAACAATTC 
TTGAGAACAA TGGCACAACC CACAGACCCT AAAGACCAAG AGGGTGTATC TGTTTGGAGT 
TATGAAGATT TAATAAAGCA CCAGAATAGC AGAAAAGATC AAGGTGGATT TATTGAAAAC 
GAAAACAAAG AAGGAGAACA GGATGAAGAT GAAGATGAAG ATATAGAAAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011098.3
CDS	1..2337
Translation	MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGNSILEMPSGTGKTVSLLSLTIAYQMHYPEHRKIIYCSRTMSEIEKALVELENLMDYRTKELGYQEDFRGLGLTSRKNLCLHPEVSKERKGTVVDEKCRRMTNGQAKRKLEEDPEANVELCEYHENLYNIEVEDYLPKGVFSFEKLLKYCEEKTLCPYFIVRRMISLCNIIIYSYHYLLDPKIAERVSNEVSKDSIVIFDEAHNIDNVCIESLSLDLTTDALRRATRGANALDERISEVRKVDSQKLQDEYEKLVQGLHSADILTDQEEPFVETPVLPQDLLTEAIPGNIRRAEHFVSFLKRLIEYLKTRMKVLHVISETPKSFLQHLKQLTFIERKPLRFCSERLSLLVRTLEVTEVEDFTALKDIATFATLISTYEEGFLLIIEPYEIENAAVPNPIMRFTCLDASIAIKPVFERFSSVIITSGTISPLDMYPRMLNFKTVLQKSYAMTLAKKSFLPMIITKGSDQVAISSRFEIRNDPSIVRNYGSMLVEFAKITPDGMVVFFPSYLYMESIVSMWQTMGILDEVWKHKLILVETPDAQETSLALETYRKACSNGRGAILLSVARGKVSEGIDFDHQYGRTVLMIGIPFQYTESRILKARLEFMRENYRIRENDFLSFDAMRHAAQCLGRVLRGKDDYGVMVLADRRFSRKRSQLPKWIAQGLSDADLNLSTDMAISNTKQFLRTMAQPTDPKDQEGVSVWSYEDLIKHQNSRKDQGGFIENENKEGEQDEDEDEDIEMQ

Target ORF information:

RefSeq Version	NM_001179061.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3 (RAD3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179061.3

ORF Insert Sequence:

ATGAAGTTTT ATATAGATGA TTTACCAGTG CTTTTTCCAT ACCCCAAGAT ATATCCAGAG 
CAGTATAATT ATATGTGCGA TATTAAAAAG ACTCTGGATG TAGGTGGAAA TAGTATCTTG 
GAGATGCCTT CAGGAACAGG TAAAACGGTC TCATTACTAT CCCTCACAAT TGCCTACCAG 
ATGCACTACC CAGAACATAG AAAGATCATA TATTGTTCTC GTACTATGTC TGAAATTGAA 
AAAGCTTTAG TAGAGTTAGA GAACCTTATG GATTACAGAA CTAAAGAACT AGGCTATCAA 
GAGGATTTTC GAGGTCTTGG CTTGACATCA AGAAAAAATT TGTGTTTGCA TCCCGAAGTG 
AGTAAGGAAC GAAAAGGTAC AGTAGTCGAT GAAAAGTGCC GTAGAATGAC AAATGGGCAG 
GCGAAGAGAA AATTAGAAGA GGATCCAGAG GCAAATGTAG AATTGTGTGA ATACCATGAG 
AATTTGTACA ATATTGAAGT AGAGGATTAT CTTCCAAAAG GCGTATTTTC TTTTGAAAAA 
CTTTTGAAAT ACTGCGAAGA AAAAACACTT TGTCCATATT TTATTGTTCG TCGTATGATT 
TCTCTTTGTA ACATTATTAT TTATTCTTAC CATTATCTAT TAGATCCTAA AATTGCTGAA 
AGAGTTTCCA ACGAGGTTTC TAAAGATAGC ATTGTCATTT TTGATGAAGC GCACAATATT 
GATAATGTGT GTATCGAATC GCTGTCATTA GACTTGACAA CGGATGCATT GAGAAGAGCC 
ACACGAGGTG CTAATGCGTT AGATGAACGT ATTTCTGAGG TCAGAAAGGT TGACTCACAG 
AAATTACAGG ATGAATACGA AAAACTAGTT CAAGGTCTCC ATTCTGCAGA TATTCTTACC 
GACCAGGAAG AGCCATTTGT GGAAACACCT GTATTGCCCC AAGATCTTCT AACAGAAGCA 
ATCCCGGGAA ATATACGAAG AGCCGAGCAT TTTGTTTCAT TTTTGAAAAG ATTGATAGAA 
TATCTGAAGA CCAGAATGAA AGTTCTTCAC GTTATTTCAG AAACGCCAAA ATCATTTCTA 
CAGCATTTAA AACAGTTAAC TTTCATAGAA AGGAAACCTC TTCGGTTTTG CTCAGAAAGG 
CTATCATTAC TTGTAAGAAC TTTAGAAGTT ACAGAGGTAG AAGATTTTAC TGCATTGAAA 
GACATAGCAA CTTTTGCTAC TCTTATATCA ACATATGAGG AAGGGTTTTT ACTAATTATT 
GAACCGTATG AAATCGAAAA TGCTGCAGTT CCGAATCCGA TTATGAGATT TACTTGCTTA 
GATGCATCGA TTGCCATCAA ACCAGTCTTT GAGAGATTTT CTTCCGTTAT TATCACTTCA 
GGGACCATAT CACCATTAGA CATGTATCCA AGAATGTTAA ACTTTAAAAC TGTTTTACAA 
AAATCATATG CCATGACCTT AGCCAAAAAA TCATTTCTAC CAATGATTAT TACCAAGGGT 
TCTGATCAAG TTGCAATTTC TTCAAGATTT GAAATCAGAA ACGATCCTAG TATTGTTCGT 
AATTACGGTT CTATGCTAGT AGAGTTTGCC AAGATCACAC CTGATGGAAT GGTTGTTTTT 
TTCCCCTCAT ATCTATATAT GGAAAGTATT GTTTCAATGT GGCAAACAAT GGGTATTCTT 
GACGAAGTTT GGAAACATAA ATTAATTTTA GTTGAGACTC CTGATGCTCA AGAAACTTCT 
TTAGCCTTAG AAACCTATAG AAAGGCTTGC TCAAATGGGC GTGGGGCAAT TTTGCTTTCT 
GTTGCTAGAG GAAAGGTATC TGAAGGTATC GATTTTGATC ATCAATATGG CAGAACTGTG 
CTGATGATAG GTATCCCGTT TCAATACACA GAATCGCGTA TTTTGAAAGC TCGCCTAGAA 
TTCATGAGGG AGAACTATCG CATCAGAGAA AACGACTTCT TATCTTTCGA TGCGATGAGA 
CATGCAGCTC AATGTCTGGG GAGAGTACTG AGAGGGAAGG ACGACTATGG TGTAATGGTA 
CTAGCAGACC GTAGGTTTTC AAGAAAAAGA AGCCAGTTAC CAAAATGGAT TGCTCAAGGT 
TTGTCTGACG CCGATTTGAA CCTTTCGACT GACATGGCCA TATCCAATAC CAAACAATTC 
TTGAGAACAA TGGCACAACC CACAGACCCT AAAGACCAAG AGGGTGTATC TGTTTGGAGT 
TATGAAGATT TAATAAAGCA CCAGAATAGC AGAAAAGATC AAGGTGGATT TATTGAAAAC 
GAAAACAAAG AAGGAGAACA GGATGAAGAT GAAGATGAAG ATATAGAAAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RAD3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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