ERCC2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERCC2
Entrez Gene ID	100125238
Full Name	ERCC excision repair 2, TFIIH core complex helicase subunit
General protein information	Preferred Names ERCC excision repair 2, TFIIH core complex helicase subunit Names TFIIH basal transcription factor complex helicase XPD subunit CXPD DNA excision repair protein ERCC-2 DNA repair protein complementing XP-D cells TFIIH basal transcription factor complex helicase subunit excision repair cross-complementing rodent repair deficiency, complementation group 2 (xeroderma pigmentosum D) excision repair cross-complementing rodent repair deficiency, complementation group 6-like group 2 xeroderma pigmentosum group D-complementing protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024977904.1	XP_024833672.1	general transcription and DNA repair factor IIH helicase subunit XPD isoform X2
XM_015458136.2	XP_015313622.1	general transcription and DNA repair factor IIH helicase subunit XPD isoform X1
NM_001103317.1	NP_001096787.1	general transcription and DNA repair factor IIH helicase subunit XPD
XM_015458136.1	XP_015313622.1	TFIIH basal transcription factor complex helicase XPD subunit isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ercc2	NP_031975.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002988	XP_311900.4
Bos taurus (cattle)	ERCC2	NP_001096787.1
Magnaporthe oryzae (rice blast fungus)	MGG_03132	XP_003716866.1
Pan troglodytes (chimpanzee)	ERCC2	NP_001233519.1
Danio rerio (zebrafish)	ercc2	NP_957220.1
Drosophila melanogaster (fruit fly)	Xpd	NP_726036.2
Schizosaccharomyces pombe (fission yeast)	rad15	NP_593025.1
Oryza sativa (rice)	Os05g0144800	NP_001054627.1
Xenopus tropicalis (tropical clawed frog)	ercc2	NP_001008131.1
Homo sapiens (human)	ERCC2	NP_000391.1
Canis lupus familiaris (dog)	ERCC2	XP_541562.3
Kluyveromyces lactis	KLLA0C17776g	XP_452994.1
Neurospora crassa	NCU01625	XP_956536.2
Arabidopsis thaliana (thale cress)	UVH6	NP_171818.1
	LOC715485	XP_002808245.1
Rattus norvegicus (Norway rat)	Ercc2	NP_001166280.1
Caenorhabditis elegans (roundworm)	Y50D7A.2	NP_497182.2
Saccharomyces cerevisiae (baker's yeast)	RAD3	NP_011098.3
Eremothecium gossypii	AGOS_AGR114C	NP_986780.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ERCC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERCC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44136	NM_001103317.1 Latest version!	Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa224763	XM_024977904.1 Latest version!	Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa93477	XM_015458136.2 Latest version!	Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa93477	XM_015458136.1	Bos taurus excision repair cross-complementation group 2 (ERCC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERCC2 ( NM_001103317.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096787.1 Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), mRNA.

Price & Availability↑

CloneID	OBa44136
Clone ID Related Accession (Same CDS sequence)	NM_001103317.1
Accession Version	NM_001103317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	general transcription and DNA repair factor IIH helicase subunit XPD
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC147982.1. ##Evidence-Data-START## Transcript exon combination :: BC147982.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGGAA 
TTTGATGTCC ACGGGCGCCA GGTGCCCCTC CCCACGGGCA TCTACAACCT AGATGACCTG 
AAGGCTGTGG GACGGCGCCA GGGCTGGTGC CCGTACTTCC TGGCCCGATA CTCAATCCTG 
CACGCCAACG TGGTGGTCTA CAGCTACCAC TACCTGCTGG ACCCCAAGAT TGCTGACCTG 
GTGTCCAAGG AGCTGGCCCG CAAGGCCGTG GTCGTCTTTG ATGAGGCCCA CAACATCGAC 
AACGTCTGCA TCGACTCCAT GAGCGTCAAC CTCACCCGCC GCACCCTGGA TCGGTGCCAG 
GCCAACCTGG AGACCCTGCA GAAGACGGTG CTCAGAATCA AGGAGACGGA CGAGCAGCGA 
CTGCGAGAGG AGTACCGCCG CCTGGTGGAG GGGCTGCGGG AGGCCAGCGC TGCCCGGGAG 
ACCGATGCCC ACCTGGCCAA CCCCGTGCTG CCTGACGAAG TGCTAAAGGA GGCGGTGCCT 
GGCTCCATCC GCACGGCCGA GCACTTCCTG GGCTTCCTGC GCCGCCTGTT GGAGTACGTC 
AAGTGGCGCC TGCGGGTGCA GCATGTGGTC CAGGAGAGCC CGCCCGCCTT CCTCAGTGGC 
CTGGCCCAGC GCGTCTGCAT CCAGCGCAAG CCCCTCAGGT TCTGCGCCGA GCGCCTCCGC 
TCCCTCCTGT ACACCTTGGA GATCTCGGAC CTCACTGACT TCTCCCCACT CACCCTCCTC 
GCTAACTTCG CCACGCTCGT CAGCACCTAT GCCAAGGGTT TCACCATCAT CATCGAGCCG 
TTCGATGACA GGACCCCGAC CATTGCCAAC CCCATCCTGC ACTTCAGCTG CATGGACGCC 
TCACTGGCCA TCAAACCTGT GTTTGAGCGC TTCCAGTCCG TCATCATCAC ATCTGGGACA 
CTGTCCCCAC TGGACATCTA CCCCAAGATC CTGGACTTCC ACCCTGTCAC CATGGCAACC 
TTCACCATGA CGTTGGCCCG GGTCTGCCTC TGCCCCATGA TCATCGGCCG TGGCAATGAC 
CAGGTGGCCA TCAGCTCCAA GTTTGAGACC CGGGAAGATA TTGCGGTGAT CCGGAACTAT 
GGAAATCTCC TGCTGGAGAT GTCTGCCGTG GTCCCTGACG GCATCGTGGC CTTCTTCACC 
AGCTACCAAT ACATGGAGAG CACTGTGGCC TCCTGGTACG AGCAGGGCAT CCTGGAGAAC 
ATCCAGAGGA ACAAGCTGCT CTTCATCGAG ACCCAGGATG GGGCTGAGAC CAGCGTCGCC 
CTAGAGAAGT ACCAGGAGGC CTGCGAGAAC GGCCGCGGGG CCATCCTGCT CTCGGTGGCC 
CGAGGCAAGG TGTCCGAGGG AATCGACTTT GTGCACCACT ACGGGCGGGC CGTCATCATG 
TTCGGGGTGC CCTACGTGTA CACCCAGAGC CGCATTCTCA AGGCACGGCT GGAATACCTT 
CGGGACCAGT TCCAGATTCG TGAGAATGAC TTTCTCACCT TTGATGCCAT GCGCCATGCT 
GCCCAGTGTG TGGGTCGGGC CATCAGGGGC AAGACGGACT ATGGCCTGAT GGTCTTTGCC 
GACAAGCGGT TTGCTCGGGC TGACAAGCGG GGGAAGCTAC CCCGGTGGAT CCAGGAGCAC 
CTCACGGACG CCAACCTCAA CCTGACCGTC GACGAGGGGG TCCAAGTCGC CAAGTACTTC 
CTGAGGCAGA TGGCACAGCC CTTCCACCGG GAGGACCAGC TGGGCCTGTC CCTGCTCAGC 
CTGGAGCAGC TGGAGTCAGA GGAGACGCTG CGGAGGATCG AGCAGATTGC CCAGCAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096787.1
CDS	9..2291
Translation	MKLNVDGLLVYFPYDYIYPEQFSYMLELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLSFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQRDSSLPHCRFYEEFDVHGRQVPLPTGIYNLDDLKAVGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQANLETLQKTVLRIKETDEQRLREEYRRLVEGLREASAARETDAHLANPVLPDEVLKEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESPPAFLSGLAQRVCIQRKPLRFCAERLRSLLYTLEISDLTDFSPLTLLANFATLVSTYAKGFTIIIEPFDDRTPTIANPILHFSCMDASLAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMATFTMTLARVCLCPMIIGRGNDQVAISSKFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFTSYQYMESTVASWYEQGILENIQRNKLLFIETQDGAETSVALEKYQEACENGRGAILLSVARGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARADKRGKLPRWIQEHLTDANLNLTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLSLEQLESEETLRRIEQIAQQL

Target ORF information:

RefSeq Version	NM_001103317.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103317.1

ORF Insert Sequence:

ATGAAGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGGAA 
TTTGATGTCC ACGGGCGCCA GGTGCCCCTC CCCACGGGCA TCTACAACCT AGATGACCTG 
AAGGCTGTGG GACGGCGCCA GGGCTGGTGC CCGTACTTCC TGGCCCGATA CTCAATCCTG 
CACGCCAACG TGGTGGTCTA CAGCTACCAC TACCTGCTGG ACCCCAAGAT TGCTGACCTG 
GTGTCCAAGG AGCTGGCCCG CAAGGCCGTG GTCGTCTTTG ATGAGGCCCA CAACATCGAC 
AACGTCTGCA TCGACTCCAT GAGCGTCAAC CTCACCCGCC GCACCCTGGA TCGGTGCCAG 
GCCAACCTGG AGACCCTGCA GAAGACGGTG CTCAGAATCA AGGAGACGGA CGAGCAGCGA 
CTGCGAGAGG AGTACCGCCG CCTGGTGGAG GGGCTGCGGG AGGCCAGCGC TGCCCGGGAG 
ACCGATGCCC ACCTGGCCAA CCCCGTGCTG CCTGACGAAG TGCTAAAGGA GGCGGTGCCT 
GGCTCCATCC GCACGGCCGA GCACTTCCTG GGCTTCCTGC GCCGCCTGTT GGAGTACGTC 
AAGTGGCGCC TGCGGGTGCA GCATGTGGTC CAGGAGAGCC CGCCCGCCTT CCTCAGTGGC 
CTGGCCCAGC GCGTCTGCAT CCAGCGCAAG CCCCTCAGGT TCTGCGCCGA GCGCCTCCGC 
TCCCTCCTGT ACACCTTGGA GATCTCGGAC CTCACTGACT TCTCCCCACT CACCCTCCTC 
GCTAACTTCG CCACGCTCGT CAGCACCTAT GCCAAGGGTT TCACCATCAT CATCGAGCCG 
TTCGATGACA GGACCCCGAC CATTGCCAAC CCCATCCTGC ACTTCAGCTG CATGGACGCC 
TCACTGGCCA TCAAACCTGT GTTTGAGCGC TTCCAGTCCG TCATCATCAC ATCTGGGACA 
CTGTCCCCAC TGGACATCTA CCCCAAGATC CTGGACTTCC ACCCTGTCAC CATGGCAACC 
TTCACCATGA CGTTGGCCCG GGTCTGCCTC TGCCCCATGA TCATCGGCCG TGGCAATGAC 
CAGGTGGCCA TCAGCTCCAA GTTTGAGACC CGGGAAGATA TTGCGGTGAT CCGGAACTAT 
GGAAATCTCC TGCTGGAGAT GTCTGCCGTG GTCCCTGACG GCATCGTGGC CTTCTTCACC 
AGCTACCAAT ACATGGAGAG CACTGTGGCC TCCTGGTACG AGCAGGGCAT CCTGGAGAAC 
ATCCAGAGGA ACAAGCTGCT CTTCATCGAG ACCCAGGATG GGGCTGAGAC CAGCGTCGCC 
CTAGAGAAGT ACCAGGAGGC CTGCGAGAAC GGCCGCGGGG CCATCCTGCT CTCGGTGGCC 
CGAGGCAAGG TGTCCGAGGG AATCGACTTT GTGCACCACT ACGGGCGGGC CGTCATCATG 
TTCGGGGTGC CCTACGTGTA CACCCAGAGC CGCATTCTCA AGGCACGGCT GGAATACCTT 
CGGGACCAGT TCCAGATTCG TGAGAATGAC TTTCTCACCT TTGATGCCAT GCGCCATGCT 
GCCCAGTGTG TGGGTCGGGC CATCAGGGGC AAGACGGACT ATGGCCTGAT GGTCTTTGCC 
GACAAGCGGT TTGCTCGGGC TGACAAGCGG GGGAAGCTAC CCCGGTGGAT CCAGGAGCAC 
CTCACGGACG CCAACCTCAA CCTGACCGTC GACGAGGGGG TCCAAGTCGC CAAGTACTTC 
CTGAGGCAGA TGGCACAGCC CTTCCACCGG GAGGACCAGC TGGGCCTGTC CCTGCTCAGC 
CTGGAGCAGC TGGAGTCAGA GGAGACGCTG CGGAGGATCG AGCAGATTGC CCAGCAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERCC2 ( XM_024977904.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833672.1 Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa224763
Clone ID Related Accession (Same CDS sequence)	XM_024977904.1
Accession Version	XM_024977904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	general transcription and DNA repair factor IIH helicase subunit XPD isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGGGA CGGACCAGCC GGCCGGTGCT CGGCGGCAAC CGCTCTCGGC CCGGCGGCAG 
GGGAGTGTGG GGGCTGGAGA GGGGCTGAGG GGACGGAGGA CTGATCGGGC AGCCCCCGCC 
TCCAGGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGATC 
CTGCACGCCA ACGTGGTGGT CTACAGCTAC CACTACCTGC TGGACCCCAA GATTGCTGAC 
CTGGTGTCCA AGGAGCTGGC CCGCAAGGCC GTGGTCGTCT TTGATGAGGC CCACAACATC 
GACAACGTCT GCATCGACTC CATGAGCGTC AACCTCACCC GCCGCACCCT GGATCGGTGC 
CAGGCCAACC TGGAGACCCT GCAGAAGACG GTGCTCAGAA TCAAGGAGAC GGACGAGCAG 
CGACTGCGAG AGGAGTACCG CCGCCTGGTG GAGGGGCTGC GGGAGGCCAG CGCTGCCCGG 
GAGACCGATG CCCACCTGGC CAACCCCGTG CTGCCTGACG AAGTGCTAAA GGAGGCGGTG 
CCTGGCTCCA TCCGCACGGC CGAGCACTTC CTGGGCTTCC TGCGCCGCCT GTTGGAGTAC 
GTCAAGTGGC GCCTGCGGGT GCAGCATGTG GTCCAGGAGA GCCCGCCCGC CTTCCTCAGT 
GGCCTGGCCC AGCGCGTCTG CATCCAGCGC AAGCCCCTCA GGTTCTGCGC CGAGCGCCTC 
CGCTCCCTCC TGTACACCTT GGAGATCTCG GACCTCACTG ACTTCTCCCC ACTCACCCTC 
CTCGCTAACT TCGCCACGCT CGTCAGCACC TATGCCAAGG GTTTCACCAT CATCATCGAG 
CCGTTCGATG ACAGGACCCC GACCATTGCC AACCCCATCC TGCACTTCAG CTGCATGGAC 
GCCTCACTGG CCATCAAACC TGTGTTTGAG CGCTTCCAGT CCGTCATCAT CACATCTGGG 
ACACTGTCCC CACTGGACAT CTACCCCAAG ATCCTGGACT TCCACCCTGT CACCATGGCA 
ACCTTCACCA TGACGTTGGC CCGGGTCTGC CTCTGCCCCA TGATCATCGG CCGTGGCAAT 
GACCAGGTGG CCATCAGCTC CAAGTTTGAG ACCCGGGAAG ATATTGCGGT GATCCGGAAC 
TATGGAAATC TCCTGCTGGA GATGTCTGCC GTGGTCCCTG ACGGCATCGT GGCCTTCTTC 
ACCAGCTACC AATACATGGA GAGCACTGTG GCCTCCTGGT ACGAGCAGGG CATCCTGGAG 
AACATCCAGA GGAACAAGCT GCTCTTCATC GAGACCCAGG ATGGGGCTGA GACCAGCGTC 
GCCCTAGAGA AGTACCAGGA GGCCTGCGAG AACGGCCGCG GGGCCATCCT GCTCTCGGTG 
GCCCGAGGCA AGGTGTCCGA GGGAATCGAC TTTGTGCACC ACTACGGGCG GGCCGTCATC 
ATGTTCGGGG TGCCCTACGT GTACACCCAG AGCCGCATTC TCAAGGCACG GCTGGAATAC 
CTTCGGGACC AGTTCCAGAT TCGTGAGAAT GACTTTCTCA CCTTTGATGC CATGCGCCAT 
GCTGCCCAGT GTGTGGGTCG GGCCATCAGG GGCAAGACGG ACTATGGCCT GATGGTCTTT 
GCCGACAAGC GGTTTGCTCG GGCTGACAAG CGGGGGAAGC TACCCCGGTG GATCCAGGAG 
CACCTCACGG ACGCCAACCT CAACCTGACC GTCGACGAGG GGGTCCAAGT CGCCAAGTAC 
TTCCTGAGGC AGATGGCACA GCCCTTCCAC CGGGAGGACC AGCTGGGCCT GTCCCTGCTC 
AGCCTGGAGC AGCTGGAGTC AGAGGAGACG CTGCGGAGGA TCGAGCAGAT TGCCCAGCAG 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833672.1
CDS	36..2321
Translation	MCGTDQPAGARRQPLSARRQGSVGAGEGLRGRRTDRAAPASRLNVDGLLVYFPYDYIYPEQFSYMLELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLSFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQRDSSLPHCRFYEILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQANLETLQKTVLRIKETDEQRLREEYRRLVEGLREASAARETDAHLANPVLPDEVLKEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESPPAFLSGLAQRVCIQRKPLRFCAERLRSLLYTLEISDLTDFSPLTLLANFATLVSTYAKGFTIIIEPFDDRTPTIANPILHFSCMDASLAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMATFTMTLARVCLCPMIIGRGNDQVAISSKFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFTSYQYMESTVASWYEQGILENIQRNKLLFIETQDGAETSVALEKYQEACENGRGAILLSVARGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARADKRGKLPRWIQEHLTDANLNLTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLSLEQLESEETLRRIEQIAQQL

Target ORF information:

RefSeq Version	XM_024977904.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977904.1

ORF Insert Sequence:

ATGTGCGGGA CGGACCAGCC GGCCGGTGCT CGGCGGCAAC CGCTCTCGGC CCGGCGGCAG 
GGGAGTGTGG GGGCTGGAGA GGGGCTGAGG GGACGGAGGA CTGATCGGGC AGCCCCCGCC 
TCCAGGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGATC 
CTGCACGCCA ACGTGGTGGT CTACAGCTAC CACTACCTGC TGGACCCCAA GATTGCTGAC 
CTGGTGTCCA AGGAGCTGGC CCGCAAGGCC GTGGTCGTCT TTGATGAGGC CCACAACATC 
GACAACGTCT GCATCGACTC CATGAGCGTC AACCTCACCC GCCGCACCCT GGATCGGTGC 
CAGGCCAACC TGGAGACCCT GCAGAAGACG GTGCTCAGAA TCAAGGAGAC GGACGAGCAG 
CGACTGCGAG AGGAGTACCG CCGCCTGGTG GAGGGGCTGC GGGAGGCCAG CGCTGCCCGG 
GAGACCGATG CCCACCTGGC CAACCCCGTG CTGCCTGACG AAGTGCTAAA GGAGGCGGTG 
CCTGGCTCCA TCCGCACGGC CGAGCACTTC CTGGGCTTCC TGCGCCGCCT GTTGGAGTAC 
GTCAAGTGGC GCCTGCGGGT GCAGCATGTG GTCCAGGAGA GCCCGCCCGC CTTCCTCAGT 
GGCCTGGCCC AGCGCGTCTG CATCCAGCGC AAGCCCCTCA GGTTCTGCGC CGAGCGCCTC 
CGCTCCCTCC TGTACACCTT GGAGATCTCG GACCTCACTG ACTTCTCCCC ACTCACCCTC 
CTCGCTAACT TCGCCACGCT CGTCAGCACC TATGCCAAGG GTTTCACCAT CATCATCGAG 
CCGTTCGATG ACAGGACCCC GACCATTGCC AACCCCATCC TGCACTTCAG CTGCATGGAC 
GCCTCACTGG CCATCAAACC TGTGTTTGAG CGCTTCCAGT CCGTCATCAT CACATCTGGG 
ACACTGTCCC CACTGGACAT CTACCCCAAG ATCCTGGACT TCCACCCTGT CACCATGGCA 
ACCTTCACCA TGACGTTGGC CCGGGTCTGC CTCTGCCCCA TGATCATCGG CCGTGGCAAT 
GACCAGGTGG CCATCAGCTC CAAGTTTGAG ACCCGGGAAG ATATTGCGGT GATCCGGAAC 
TATGGAAATC TCCTGCTGGA GATGTCTGCC GTGGTCCCTG ACGGCATCGT GGCCTTCTTC 
ACCAGCTACC AATACATGGA GAGCACTGTG GCCTCCTGGT ACGAGCAGGG CATCCTGGAG 
AACATCCAGA GGAACAAGCT GCTCTTCATC GAGACCCAGG ATGGGGCTGA GACCAGCGTC 
GCCCTAGAGA AGTACCAGGA GGCCTGCGAG AACGGCCGCG GGGCCATCCT GCTCTCGGTG 
GCCCGAGGCA AGGTGTCCGA GGGAATCGAC TTTGTGCACC ACTACGGGCG GGCCGTCATC 
ATGTTCGGGG TGCCCTACGT GTACACCCAG AGCCGCATTC TCAAGGCACG GCTGGAATAC 
CTTCGGGACC AGTTCCAGAT TCGTGAGAAT GACTTTCTCA CCTTTGATGC CATGCGCCAT 
GCTGCCCAGT GTGTGGGTCG GGCCATCAGG GGCAAGACGG ACTATGGCCT GATGGTCTTT 
GCCGACAAGC GGTTTGCTCG GGCTGACAAG CGGGGGAAGC TACCCCGGTG GATCCAGGAG 
CACCTCACGG ACGCCAACCT CAACCTGACC GTCGACGAGG GGGTCCAAGT CGCCAAGTAC 
TTCCTGAGGC AGATGGCACA GCCCTTCCAC CGGGAGGACC AGCTGGGCCT GTCCCTGCTC 
AGCCTGGAGC AGCTGGAGTC AGAGGAGACG CTGCGGAGGA TCGAGCAGAT TGCCCAGCAG 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERCC2 ( XM_015458136.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313622.1 Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa93477
Clone ID Related Accession (Same CDS sequence)	XM_015458136.2 , XM_015458136.1
Accession Version	XM_015458136.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	general transcription and DNA repair factor IIH helicase subunit XPD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGGGA CGGACCAGCC GGCCGGTGCT CGGCGGCAAC CGCTCTCGGC CCGGCGGCAG 
GGGAGTGTGG GGGCTGGAGA GGGGCTGAGG GGACGGAGGA CTGATCGGGC AGCCCCCGCC 
TCCAGGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGGAA 
TTTGATGTCC ACGGGCGCCA GGTGCCCCTC CCCACGGGCA TCTACAACCT AGATGACCTG 
AAGGCTGTGG GACGGCGCCA GGGCTGGTGC CCGTACTTCC TGGCCCGATA CTCAATCCTG 
CACGCCAACG TGGTGGTCTA CAGCTACCAC TACCTGCTGG ACCCCAAGAT TGCTGACCTG 
GTGTCCAAGG AGCTGGCCCG CAAGGCCGTG GTCGTCTTTG ATGAGGCCCA CAACATCGAC 
AACGTCTGCA TCGACTCCAT GAGCGTCAAC CTCACCCGCC GCACCCTGGA TCGGTGCCAG 
GCCAACCTGG AGACCCTGCA GAAGACGGTG CTCAGAATCA AGGAGACGGA CGAGCAGCGA 
CTGCGAGAGG AGTACCGCCG CCTGGTGGAG GGGCTGCGGG AGGCCAGCGC TGCCCGGGAG 
ACCGATGCCC ACCTGGCCAA CCCCGTGCTG CCTGACGAAG TGCTAAAGGA GGCGGTGCCT 
GGCTCCATCC GCACGGCCGA GCACTTCCTG GGCTTCCTGC GCCGCCTGTT GGAGTACGTC 
AAGTGGCGCC TGCGGGTGCA GCATGTGGTC CAGGAGAGCC CGCCCGCCTT CCTCAGTGGC 
CTGGCCCAGC GCGTCTGCAT CCAGCGCAAG CCCCTCAGGT TCTGCGCCGA GCGCCTCCGC 
TCCCTCCTGT ACACCTTGGA GATCTCGGAC CTCACTGACT TCTCCCCACT CACCCTCCTC 
GCTAACTTCG CCACGCTCGT CAGCACCTAT GCCAAGGGTT TCACCATCAT CATCGAGCCG 
TTCGATGACA GGACCCCGAC CATTGCCAAC CCCATCCTGC ACTTCAGCTG CATGGACGCC 
TCACTGGCCA TCAAACCTGT GTTTGAGCGC TTCCAGTCCG TCATCATCAC ATCTGGGACA 
CTGTCCCCAC TGGACATCTA CCCCAAGATC CTGGACTTCC ACCCTGTCAC CATGGCAACC 
TTCACCATGA CGTTGGCCCG GGTCTGCCTC TGCCCCATGA TCATCGGCCG TGGCAATGAC 
CAGGTGGCCA TCAGCTCCAA GTTTGAGACC CGGGAAGATA TTGCGGTGAT CCGGAACTAT 
GGAAATCTCC TGCTGGAGAT GTCTGCCGTG GTCCCTGACG GCATCGTGGC CTTCTTCACC 
AGCTACCAAT ACATGGAGAG CACTGTGGCC TCCTGGTACG AGCAGGGCAT CCTGGAGAAC 
ATCCAGAGGA ACAAGCTGCT CTTCATCGAG ACCCAGGATG GGGCTGAGAC CAGCGTCGCC 
CTAGAGAAGT ACCAGGAGGC CTGCGAGAAC GGCCGCGGGG CCATCCTGCT CTCGGTGGCC 
CGAGGCAAGG TGTCCGAGGG AATCGACTTT GTGCACCACT ACGGGCGGGC CGTCATCATG 
TTCGGGGTGC CCTACGTGTA CACCCAGAGC CGCATTCTCA AGGCACGGCT GGAATACCTT 
CGGGACCAGT TCCAGATTCG TGAGAATGAC TTTCTCACCT TTGATGCCAT GCGCCATGCT 
GCCCAGTGTG TGGGTCGGGC CATCAGGGGC AAGACGGACT ATGGCCTGAT GGTCTTTGCC 
GACAAGCGGT TTGCTCGGGC TGACAAGCGG GGGAAGCTAC CCCGGTGGAT CCAGGAGCAC 
CTCACGGACG CCAACCTCAA CCTGACCGTC GACGAGGGGG TCCAAGTCGC CAAGTACTTC 
CTGAGGCAGA TGGCACAGCC CTTCCACCGG GAGGACCAGC TGGGCCTGTC CCTGCTCAGC 
CTGGAGCAGC TGGAGTCAGA GGAGACGCTG CGGAGGATCG AGCAGATTGC CCAGCAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313622.1
CDS	34..2436
Translation	MCGTDQPAGARRQPLSARRQGSVGAGEGLRGRRTDRAAPASRLNVDGLLVYFPYDYIYPEQFSYMLELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLSFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQRDSSLPHCRFYEEFDVHGRQVPLPTGIYNLDDLKAVGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQANLETLQKTVLRIKETDEQRLREEYRRLVEGLREASAARETDAHLANPVLPDEVLKEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESPPAFLSGLAQRVCIQRKPLRFCAERLRSLLYTLEISDLTDFSPLTLLANFATLVSTYAKGFTIIIEPFDDRTPTIANPILHFSCMDASLAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMATFTMTLARVCLCPMIIGRGNDQVAISSKFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFTSYQYMESTVASWYEQGILENIQRNKLLFIETQDGAETSVALEKYQEACENGRGAILLSVARGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARADKRGKLPRWIQEHLTDANLNLTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLSLEQLESEETLRRIEQIAQQL

Target ORF information:

RefSeq Version	XM_015458136.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458136.2

ORF Insert Sequence:

ATGTGCGGGA CGGACCAGCC GGCCGGTGCT CGGCGGCAAC CGCTCTCGGC CCGGCGGCAG 
GGGAGTGTGG GGGCTGGAGA GGGGCTGAGG GGACGGAGGA CTGATCGGGC AGCCCCCGCC 
TCCAGGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGGAA 
TTTGATGTCC ACGGGCGCCA GGTGCCCCTC CCCACGGGCA TCTACAACCT AGATGACCTG 
AAGGCTGTGG GACGGCGCCA GGGCTGGTGC CCGTACTTCC TGGCCCGATA CTCAATCCTG 
CACGCCAACG TGGTGGTCTA CAGCTACCAC TACCTGCTGG ACCCCAAGAT TGCTGACCTG 
GTGTCCAAGG AGCTGGCCCG CAAGGCCGTG GTCGTCTTTG ATGAGGCCCA CAACATCGAC 
AACGTCTGCA TCGACTCCAT GAGCGTCAAC CTCACCCGCC GCACCCTGGA TCGGTGCCAG 
GCCAACCTGG AGACCCTGCA GAAGACGGTG CTCAGAATCA AGGAGACGGA CGAGCAGCGA 
CTGCGAGAGG AGTACCGCCG CCTGGTGGAG GGGCTGCGGG AGGCCAGCGC TGCCCGGGAG 
ACCGATGCCC ACCTGGCCAA CCCCGTGCTG CCTGACGAAG TGCTAAAGGA GGCGGTGCCT 
GGCTCCATCC GCACGGCCGA GCACTTCCTG GGCTTCCTGC GCCGCCTGTT GGAGTACGTC 
AAGTGGCGCC TGCGGGTGCA GCATGTGGTC CAGGAGAGCC CGCCCGCCTT CCTCAGTGGC 
CTGGCCCAGC GCGTCTGCAT CCAGCGCAAG CCCCTCAGGT TCTGCGCCGA GCGCCTCCGC 
TCCCTCCTGT ACACCTTGGA GATCTCGGAC CTCACTGACT TCTCCCCACT CACCCTCCTC 
GCTAACTTCG CCACGCTCGT CAGCACCTAT GCCAAGGGTT TCACCATCAT CATCGAGCCG 
TTCGATGACA GGACCCCGAC CATTGCCAAC CCCATCCTGC ACTTCAGCTG CATGGACGCC 
TCACTGGCCA TCAAACCTGT GTTTGAGCGC TTCCAGTCCG TCATCATCAC ATCTGGGACA 
CTGTCCCCAC TGGACATCTA CCCCAAGATC CTGGACTTCC ACCCTGTCAC CATGGCAACC 
TTCACCATGA CGTTGGCCCG GGTCTGCCTC TGCCCCATGA TCATCGGCCG TGGCAATGAC 
CAGGTGGCCA TCAGCTCCAA GTTTGAGACC CGGGAAGATA TTGCGGTGAT CCGGAACTAT 
GGAAATCTCC TGCTGGAGAT GTCTGCCGTG GTCCCTGACG GCATCGTGGC CTTCTTCACC 
AGCTACCAAT ACATGGAGAG CACTGTGGCC TCCTGGTACG AGCAGGGCAT CCTGGAGAAC 
ATCCAGAGGA ACAAGCTGCT CTTCATCGAG ACCCAGGATG GGGCTGAGAC CAGCGTCGCC 
CTAGAGAAGT ACCAGGAGGC CTGCGAGAAC GGCCGCGGGG CCATCCTGCT CTCGGTGGCC 
CGAGGCAAGG TGTCCGAGGG AATCGACTTT GTGCACCACT ACGGGCGGGC CGTCATCATG 
TTCGGGGTGC CCTACGTGTA CACCCAGAGC CGCATTCTCA AGGCACGGCT GGAATACCTT 
CGGGACCAGT TCCAGATTCG TGAGAATGAC TTTCTCACCT TTGATGCCAT GCGCCATGCT 
GCCCAGTGTG TGGGTCGGGC CATCAGGGGC AAGACGGACT ATGGCCTGAT GGTCTTTGCC 
GACAAGCGGT TTGCTCGGGC TGACAAGCGG GGGAAGCTAC CCCGGTGGAT CCAGGAGCAC 
CTCACGGACG CCAACCTCAA CCTGACCGTC GACGAGGGGG TCCAAGTCGC CAAGTACTTC 
CTGAGGCAGA TGGCACAGCC CTTCCACCGG GAGGACCAGC TGGGCCTGTC CCTGCTCAGC 
CTGGAGCAGC TGGAGTCAGA GGAGACGCTG CGGAGGATCG AGCAGATTGC CCAGCAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERCC2 ( XM_015458136.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313622.1 Bos taurus excision repair cross-complementation group 2 (ERCC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa93477
Clone ID Related Accession (Same CDS sequence)	XM_015458136.2 , XM_015458136.1
Accession Version	XM_015458136.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TFIIH basal transcription factor complex helicase XPD subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGGGA CGGACCAGCC GGCCGGTGCT CGGCGGCAAC CGCTCTCGGC CCGGCGGCAG 
GGGAGTGTGG GGGCTGGAGA GGGGCTGAGG GGACGGAGGA CTGATCGGGC AGCCCCCGCC 
TCCAGGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGGAA 
TTTGATGTCC ACGGGCGCCA GGTGCCCCTC CCCACGGGCA TCTACAACCT AGATGACCTG 
AAGGCTGTGG GACGGCGCCA GGGCTGGTGC CCGTACTTCC TGGCCCGATA CTCAATCCTG 
CACGCCAACG TGGTGGTCTA CAGCTACCAC TACCTGCTGG ACCCCAAGAT TGCTGACCTG 
GTGTCCAAGG AGCTGGCCCG CAAGGCCGTG GTCGTCTTTG ATGAGGCCCA CAACATCGAC 
AACGTCTGCA TCGACTCCAT GAGCGTCAAC CTCACCCGCC GCACCCTGGA TCGGTGCCAG 
GCCAACCTGG AGACCCTGCA GAAGACGGTG CTCAGAATCA AGGAGACGGA CGAGCAGCGA 
CTGCGAGAGG AGTACCGCCG CCTGGTGGAG GGGCTGCGGG AGGCCAGCGC TGCCCGGGAG 
ACCGATGCCC ACCTGGCCAA CCCCGTGCTG CCTGACGAAG TGCTAAAGGA GGCGGTGCCT 
GGCTCCATCC GCACGGCCGA GCACTTCCTG GGCTTCCTGC GCCGCCTGTT GGAGTACGTC 
AAGTGGCGCC TGCGGGTGCA GCATGTGGTC CAGGAGAGCC CGCCCGCCTT CCTCAGTGGC 
CTGGCCCAGC GCGTCTGCAT CCAGCGCAAG CCCCTCAGGT TCTGCGCCGA GCGCCTCCGC 
TCCCTCCTGT ACACCTTGGA GATCTCGGAC CTCACTGACT TCTCCCCACT CACCCTCCTC 
GCTAACTTCG CCACGCTCGT CAGCACCTAT GCCAAGGGTT TCACCATCAT CATCGAGCCG 
TTCGATGACA GGACCCCGAC CATTGCCAAC CCCATCCTGC ACTTCAGCTG CATGGACGCC 
TCACTGGCCA TCAAACCTGT GTTTGAGCGC TTCCAGTCCG TCATCATCAC ATCTGGGACA 
CTGTCCCCAC TGGACATCTA CCCCAAGATC CTGGACTTCC ACCCTGTCAC CATGGCAACC 
TTCACCATGA CGTTGGCCCG GGTCTGCCTC TGCCCCATGA TCATCGGCCG TGGCAATGAC 
CAGGTGGCCA TCAGCTCCAA GTTTGAGACC CGGGAAGATA TTGCGGTGAT CCGGAACTAT 
GGAAATCTCC TGCTGGAGAT GTCTGCCGTG GTCCCTGACG GCATCGTGGC CTTCTTCACC 
AGCTACCAAT ACATGGAGAG CACTGTGGCC TCCTGGTACG AGCAGGGCAT CCTGGAGAAC 
ATCCAGAGGA ACAAGCTGCT CTTCATCGAG ACCCAGGATG GGGCTGAGAC CAGCGTCGCC 
CTAGAGAAGT ACCAGGAGGC CTGCGAGAAC GGCCGCGGGG CCATCCTGCT CTCGGTGGCC 
CGAGGCAAGG TGTCCGAGGG AATCGACTTT GTGCACCACT ACGGGCGGGC CGTCATCATG 
TTCGGGGTGC CCTACGTGTA CACCCAGAGC CGCATTCTCA AGGCACGGCT GGAATACCTT 
CGGGACCAGT TCCAGATTCG TGAGAATGAC TTTCTCACCT TTGATGCCAT GCGCCATGCT 
GCCCAGTGTG TGGGTCGGGC CATCAGGGGC AAGACGGACT ATGGCCTGAT GGTCTTTGCC 
GACAAGCGGT TTGCTCGGGC TGACAAGCGG GGGAAGCTAC CCCGGTGGAT CCAGGAGCAC 
CTCACGGACG CCAACCTCAA CCTGACCGTC GACGAGGGGG TCCAAGTCGC CAAGTACTTC 
CTGAGGCAGA TGGCACAGCC CTTCCACCGG GAGGACCAGC TGGGCCTGTC CCTGCTCAGC 
CTGGAGCAGC TGGAGTCAGA GGAGACGCTG CGGAGGATCG AGCAGATTGC CCAGCAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313622.1
CDS	141..2543
Translation	MCGTDQPAGARRQPLSARRQGSVGAGEGLRGRRTDRAAPASRLNVDGLLVYFPYDYIYPEQFSYMLELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLSFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQRDSSLPHCRFYEEFDVHGRQVPLPTGIYNLDDLKAVGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQANLETLQKTVLRIKETDEQRLREEYRRLVEGLREASAARETDAHLANPVLPDEVLKEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESPPAFLSGLAQRVCIQRKPLRFCAERLRSLLYTLEISDLTDFSPLTLLANFATLVSTYAKGFTIIIEPFDDRTPTIANPILHFSCMDASLAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMATFTMTLARVCLCPMIIGRGNDQVAISSKFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFTSYQYMESTVASWYEQGILENIQRNKLLFIETQDGAETSVALEKYQEACENGRGAILLSVARGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARADKRGKLPRWIQEHLTDANLNLTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLSLEQLESEETLRRIEQIAQQL

Target ORF information:

RefSeq Version	XM_015458136.1
Organism	Bos taurus(cattle)
Definition	Bos taurus excision repair cross-complementation group 2 (ERCC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458136.1

ORF Insert Sequence:

ATGTGCGGGA CGGACCAGCC GGCCGGTGCT CGGCGGCAAC CGCTCTCGGC CCGGCGGCAG 
GGGAGTGTGG GGGCTGGAGA GGGGCTGAGG GGACGGAGGA CTGATCGGGC AGCCCCCGCC 
TCCAGGCTCA ACGTGGACGG GCTGCTGGTC TACTTCCCCT ACGACTACAT CTACCCGGAG 
CAGTTCTCGT ACATGCTGGA GCTCAAACGC ACGCTGGATG CCAAGGGTCA TGGAGTCCTG 
GAGATGCCCT CAGGCACTGG GAAGACAGTG TCCCTGTTGG CCCTGATCAT GGCGTACCAG 
CGGGCGTATC CCCTGGAGGT GACTAAACTC ATCTACTGCT CGAGGACTGT GCCTGAGATT 
GAAAAGGTGA TTGAAGAACT TCGAAAGTTA CTCAGCTTCT ATGAGAAACA GGAGGGCGAG 
AAGCTGCCAT TTTTGGGGCT GGCTCTGAGC TCCCGGAAGA ACCTGTGTAT TCATCCCGAG 
GTGACGCCCC TGCGCTTTGG GAAGGACGTG GACGGGAAAT GCCACAGCCT CACGGCATCG 
TACGTGCGGG CACAGTACCA GCGGGACTCC AGCCTGCCCC ACTGCCGCTT CTACGAGGAA 
TTTGATGTCC ACGGGCGCCA GGTGCCCCTC CCCACGGGCA TCTACAACCT AGATGACCTG 
AAGGCTGTGG GACGGCGCCA GGGCTGGTGC CCGTACTTCC TGGCCCGATA CTCAATCCTG 
CACGCCAACG TGGTGGTCTA CAGCTACCAC TACCTGCTGG ACCCCAAGAT TGCTGACCTG 
GTGTCCAAGG AGCTGGCCCG CAAGGCCGTG GTCGTCTTTG ATGAGGCCCA CAACATCGAC 
AACGTCTGCA TCGACTCCAT GAGCGTCAAC CTCACCCGCC GCACCCTGGA TCGGTGCCAG 
GCCAACCTGG AGACCCTGCA GAAGACGGTG CTCAGAATCA AGGAGACGGA CGAGCAGCGA 
CTGCGAGAGG AGTACCGCCG CCTGGTGGAG GGGCTGCGGG AGGCCAGCGC TGCCCGGGAG 
ACCGATGCCC ACCTGGCCAA CCCCGTGCTG CCTGACGAAG TGCTAAAGGA GGCGGTGCCT 
GGCTCCATCC GCACGGCCGA GCACTTCCTG GGCTTCCTGC GCCGCCTGTT GGAGTACGTC 
AAGTGGCGCC TGCGGGTGCA GCATGTGGTC CAGGAGAGCC CGCCCGCCTT CCTCAGTGGC 
CTGGCCCAGC GCGTCTGCAT CCAGCGCAAG CCCCTCAGGT TCTGCGCCGA GCGCCTCCGC 
TCCCTCCTGT ACACCTTGGA GATCTCGGAC CTCACTGACT TCTCCCCACT CACCCTCCTC 
GCTAACTTCG CCACGCTCGT CAGCACCTAT GCCAAGGGTT TCACCATCAT CATCGAGCCG 
TTCGATGACA GGACCCCGAC CATTGCCAAC CCCATCCTGC ACTTCAGCTG CATGGACGCC 
TCACTGGCCA TCAAACCTGT GTTTGAGCGC TTCCAGTCCG TCATCATCAC ATCTGGGACA 
CTGTCCCCAC TGGACATCTA CCCCAAGATC CTGGACTTCC ACCCTGTCAC CATGGCAACC 
TTCACCATGA CGTTGGCCCG GGTCTGCCTC TGCCCCATGA TCATCGGCCG TGGCAATGAC 
CAGGTGGCCA TCAGCTCCAA GTTTGAGACC CGGGAAGATA TTGCGGTGAT CCGGAACTAT 
GGAAATCTCC TGCTGGAGAT GTCTGCCGTG GTCCCTGACG GCATCGTGGC CTTCTTCACC 
AGCTACCAAT ACATGGAGAG CACTGTGGCC TCCTGGTACG AGCAGGGCAT CCTGGAGAAC 
ATCCAGAGGA ACAAGCTGCT CTTCATCGAG ACCCAGGATG GGGCTGAGAC CAGCGTCGCC 
CTAGAGAAGT ACCAGGAGGC CTGCGAGAAC GGCCGCGGGG CCATCCTGCT CTCGGTGGCC 
CGAGGCAAGG TGTCCGAGGG AATCGACTTT GTGCACCACT ACGGGCGGGC CGTCATCATG 
TTCGGGGTGC CCTACGTGTA CACCCAGAGC CGCATTCTCA AGGCACGGCT GGAATACCTT 
CGGGACCAGT TCCAGATTCG TGAGAATGAC TTTCTCACCT TTGATGCCAT GCGCCATGCT 
GCCCAGTGTG TGGGTCGGGC CATCAGGGGC AAGACGGACT ATGGCCTGAT GGTCTTTGCC 
GACAAGCGGT TTGCTCGGGC TGACAAGCGG GGGAAGCTAC CCCGGTGGAT CCAGGAGCAC 
CTCACGGACG CCAACCTCAA CCTGACCGTC GACGAGGGGG TCCAAGTCGC CAAGTACTTC 
CTGAGGCAGA TGGCACAGCC CTTCCACCGG GAGGACCAGC TGGGCCTGTC CCTGCTCAGC 
CTGGAGCAGC TGGAGTCAGA GGAGACGCTG CGGAGGATCG AGCAGATTGC CCAGCAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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ERCC2 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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