RNR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RNR1
Entrez Gene ID	856801
Full Name	ribonucleotide-diphosphate reductase subunit RNR1
Synonyms	CRT7,RIR1,SDS12
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178961.1	NP_010993.1	ribonucleotide-diphosphate reductase subunit RNR1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RRM1	XP_534027.2
Mus musculus (house mouse)	Rrm1	NP_033129.2
Gallus gallus (chicken)	RRM1	NP_001026008.1
Saccharomyces cerevisiae (baker's yeast)	RNR3	NP_012198.3
Eremothecium gossypii	AGOS_ADL057W	NP_984039.2
Schizosaccharomyces pombe (fission yeast)	cdc22	NP_594491.1
Pan troglodytes (chimpanzee)	RRM1	XP_001160029.1
Bos taurus (cattle)	RRM1	NP_001106767.1
Rattus norvegicus (Norway rat)	Rrm1	NP_001013254.1
Caenorhabditis elegans (roundworm)	rnr-1	NP_499039.1
Saccharomyces cerevisiae (baker's yeast)	RNR1	NP_010993.1
Oryza sativa (rice)	Os06g0168600	NP_001056926.1
Danio rerio (zebrafish)	rrm1	NP_571530.1
Magnaporthe oryzae (rice blast fungus)	MGG_07000	XP_003709756.1
Neurospora crassa	NCU03539	XP_956818.1
Xenopus tropicalis (tropical clawed frog)	rrm1	NP_001120566.1
Homo sapiens (human)	RRM1	NP_001024.1
	RRM1	XP_001113010.1
Drosophila melanogaster (fruit fly)	RnrL	NP_477027.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010198	XP_554103.3
Kluyveromyces lactis	KLLA0C07887g	XP_452551.1
Arabidopsis thaliana (thale cress)	RNR1	NP_179770.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fhl1p regulates RNR1 gene transcription to maintain dNTP levels, thus modulating longevity by protection against replication stress
Title: The forkhead-like transcription factor (Fhl1p) maintains yeast replicative lifespan by regulating ribonucleotide reductase 1 (RNR1) gene transcription.

Multiple turnover kinetics show that Sml1 inhibition of dGTP/ADP- and ATP/CDP-bound ScRR follows a mixed inhibition mechanism. However, Sml1 cooperatively binds to the ES complex in the dGTP/ADP form, whereas with ATP/CDP, Sml1 binds weakly and noncooperatively
Title: Inhibition of yeast ribonucleotide reductase by Sml1 depends on the allosteric state of the enzyme.

there is a directed orientation of the RNR beta and beta' C-terminal tails relative to alpha within the holoenzyme consistent with a docking model of the two subunits and argue against RT across the beta beta' interface
Title: Investigation of in vivo roles of the C-terminal tails of the small subunit (??') of Saccharomyces cerevisiae ribonucleotide reductase: contribution to cofactor formation and intersubunit association within the active holoenzyme.

The data reveaedl that the arginine 293 and glutamine 288 residues of ribonucleotide reductase 1 are crucial in facilitating ADP and CDP substrate selection.
Title: Role of arginine 293 and glutamine 288 in communication between catalytic and allosteric sites in yeast ribonucleotide reductase.

defects in autophagy result in an increase in soluble Rnr1 protein levels and a DNA damage phenotype.
Title: Autophagy-dependent regulation of the DNA damage response protein ribonucleotide reductase 1.

The following RNR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05565	NM_001178961.1 Latest version!	Saccharomyces cerevisiae S288C ribonucleotide-diphosphate reductase subunit RNR1 (RNR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNR1 ( NM_001178961.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010993.1 Saccharomyces cerevisiae S288C ribonucleotide-diphosphate reductase subunit RNR1 (RNR1), partial mRNA.

Price & Availability↑

CloneID	OSi05565
Clone ID Related Accession (Same CDS sequence)	NM_001178961.1
Accession Version	NM_001178961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ribonucleotide-diphosphate reductase subunit RNR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTACGTTT ATAAAAGAGA CGGTCGTAAA GAACCTGTCC AATTCGATAA GATTACCGCT 
CGTATATCAC GCTTATGCTA TGGTTTAGAT CCAAAACATA TCGACGCCGT TAAGGTCACC 
CAACGTATCA TTTCTGGTGT CTATGAAGGT GTCACAACAA TCGAACTAGA CAACTTAGCC 
GCTGAAACAT GCGCTTATAT GACTACTGTT CATCCAGATT ACGCCACCCT AGCGGCCAGA 
ATTGCCATTT CTAATTTACA TAAACAAACC ACAAAACAAT TTTCTAAGGT TGTCGAAGAT 
CTTTATAGAT ACGTCAATGC TGCTACTGGT AAGCCCGCTC CCATGATCTC TGATGATGTC 
TACAATATTG TCATGGAAAA CAAGGATAAA TTGAACTCCG CAATTGTCTA TGACAGAGAT 
TTTCAGTACA GTTATTTTGG TTTTAAAACT TTGGAACGTT CTTATTTACT AAGAATCAAC 
GGTCAAGTGG CCGAACGTCC ACAACATTTA ATTATGAGAG TCGCACTAGG CATCCACGGT 
AGAGATATCG AGGCTGCTTT AGAAACGTAT AACTTGATGT CTCTAAAATA TTTTACTCAC 
GCCTCTCCAA CGTTGTTCAA TGCCGGTACT CCAAAACCTC AAATGTCCTC TTGTTTCTTG 
GTTGCCATGA AGGAGGACTC TATCGAGGGG ATTTACGACA CCTTGAAGGA ATGTGCTTTG 
ATTTCCAAAA CTGCTGGTGG TATTGGTCTA CATATCCATA ACATTCGTTC AACTGGTTCT 
TACATTGCTG GTACAAACGG TACTTCTAAC GGTTTAATTC CTATGATTCG TGTTTTCAAT 
AACACTGCCC GTTATGTTGA CCAGGGTGGT AATAAAAGAC CTGGTGCGTT TGCCCTTTAC 
CTGGAACCAT GGCATGCTGA TATATTTGAT TTTATTGATA TTAGGAAGAA CCACGGTAAA 
GAGGAAATTC GTGCAAGAGA TTTGTTCCCT GCTCTATGGA TTCCTGATCT TTTCATGAAG 
CGTGTCGAAG AAAATGGGAC CTGGACATTA TTCTCTCCAA CATCAGCTCC TGGTTTAAGC 
GATTGTTACG GTGACGAGTT TGAGGCTCTA TATACCCGCT ACGAGAAAGA AGGTCGTGGT 
AAGACTATCA AAGCCCAAAA ATTATGGTAT TCCATTTTGG AAGCTCAAAC TGAAACTGGT 
ACACCTTTCG TTGTTTACAA GGATGCTTGT AACAGAAAAT CTAATCAAAA AAACTTAGGT 
GTCATCAAGT CATCAAACTT ATGCTGTGAA ATTGTTGAAT ACTCAGCTCC AGATGAAACT 
GCTGTTTGTA ACTTGGCTTC CGTTGCCTTA CCAGCATTCA TTGAAACTTC TGAGGATGGT 
AAGACTTCCA CATACAACTT CAAAAAATTA CATGAAATTG CTAAAGTTGT TACTCGTAAT 
TTAAACAGAG TCATTGATCG TAATTACTAC CCTGTTGAAG AAGCGAGAAA ATCCAATATG 
AGACATAGAC CAATTGCTTT GGGTGTTCAA GGTCTCGCTG ACACTTTCAT GCTGTTACGT 
TTGCCATTTG ATTCTGAGGA AGCCCGTTTG CTAAATATCC AAATCTTTGA AACTATTTAT 
CATGCCTCCA TGGAAGCTTC TTGTGAACTA GCTCAGAAGG ACGGTCCATA CGAAACTTTC 
CAAGGATCTC CTGCTTCTCA AGGTATACTA CAGTTTGATA TGTGGGACCA AAAACCCTAC 
GGCATGTGGG ATTGGGACAC CTTAAGAAAA GATATCATGA AGCATGGTGT TAGAAATTCC 
TTGACCATGG CACCAATGCC TACTGCATCC ACATCCCAAA TATTGGGTTA TAATGAATGT 
TTCGAACCAG TCACTTCCAA TATGTACTCC CGTCGTGTCT TATCCGGTGA ATTCCAGGTT 
GTGAACCCTT ACTTACTGCG TGACTTGGTT GATTTAGGTA TTTGGGATGA GGGTATGAAA 
CAGTATCTGA TTACACAAAA TGGCTCCATT CAAGGCTTAC CAAACGTTCC ACAAGAATTG 
AAGGACTTAT ACAAGACTGT TTGGGAAATT TCACAAAAGA CTATCATTAA CATGGCAGCC 
GATCGTTCTG TCTATATTGA TCAATCTCAT TCTTTGAATT TGTTCTTACG TGCCCCAACT 
ATGGGTAAAC TAACAAGTAT GCATTTTTAC GGATGGAAGA AGGGATTGAA GACCGGTATG 
TACTATTTGA GAACCCAAGC TGCATCTGCT GCAATTCAAT TTACTATTGA TCAGAAGATT 
GCGGATCAAG CTACAGAAAA CGTTGCTGAT ATTTCCAACT TGAAGCGTCC ATCATATATG 
CCTTCCAGTG CAAGCTACGC TGCCAGCGAT TTCGTGCCCG CAGCTGTGAC TGCAAACGCA 
ACTATTCCAT CTCTAGATAG CTCCTCGGAA GCTTCAAGAG AGGCATCTCC AGCTCCAACA 
GGTAGCCACT CATTAACTAA AGGAATGGCA GAATTAAACG TTCAAGAGTC TAAGGTAGAA 
GTTCCTGAAG TACCTGCCCC AACTAAGAAT GAAGAAAAAG CTGCCCCCAT CGTTGATGAT 
GAGGAAACCG AGTTCGACAT TTACAACTCT AAGGTTATAG CATGTGCTAT TGATAACCCA 
GAAGCTTGTG AAATGTGTTC GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010993.1
CDS	1..2667
Translation	MYVYKRDGRKEPVQFDKITARISRLCYGLDPKHIDAVKVTQRIISGVYEGVTTIELDNLAAETCAYMTTVHPDYATLAARIAISNLHKQTTKQFSKVVEDLYRYVNAATGKPAPMISDDVYNIVMENKDKLNSAIVYDRDFQYSYFGFKTLERSYLLRINGQVAERPQHLIMRVALGIHGRDIEAALETYNLMSLKYFTHASPTLFNAGTPKPQMSSCFLVAMKEDSIEGIYDTLKECALISKTAGGIGLHIHNIRSTGSYIAGTNGTSNGLIPMIRVFNNTARYVDQGGNKRPGAFALYLEPWHADIFDFIDIRKNHGKEEIRARDLFPALWIPDLFMKRVEENGTWTLFSPTSAPGLSDCYGDEFEALYTRYEKEGRGKTIKAQKLWYSILEAQTETGTPFVVYKDACNRKSNQKNLGVIKSSNLCCEIVEYSAPDETAVCNLASVALPAFIETSEDGKTSTYNFKKLHEIAKVVTRNLNRVIDRNYYPVEEARKSNMRHRPIALGVQGLADTFMLLRLPFDSEEARLLNIQIFETIYHASMEASCELAQKDGPYETFQGSPASQGILQFDMWDQKPYGMWDWDTLRKDIMKHGVRNSLTMAPMPTASTSQILGYNECFEPVTSNMYSRRVLSGEFQVVNPYLLRDLVDLGIWDEGMKQYLITQNGSIQGLPNVPQELKDLYKTVWEISQKTIINMAADRSVYIDQSHSLNLFLRAPTMGKLTSMHFYGWKKGLKTGMYYLRTQAASAAIQFTIDQKIADQATENVADISNLKRPSYMPSSASYAASDFVPAAVTANATIPSLDSSSEASREASPAPTGSHSLTKGMAELNVQESKVEVPEVPAPTKNEEKAAPIVDDEETEFDIYNSKVIACAIDNPEACEMCSG

Target ORF information:

RefSeq Version	NM_001178961.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ribonucleotide-diphosphate reductase subunit RNR1 (RNR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178961.1

ORF Insert Sequence:

ATGTACGTTT ATAAAAGAGA CGGTCGTAAA GAACCTGTCC AATTCGATAA GATTACCGCT 
CGTATATCAC GCTTATGCTA TGGTTTAGAT CCAAAACATA TCGACGCCGT TAAGGTCACC 
CAACGTATCA TTTCTGGTGT CTATGAAGGT GTCACAACAA TCGAACTAGA CAACTTAGCC 
GCTGAAACAT GCGCTTATAT GACTACTGTT CATCCAGATT ACGCCACCCT AGCGGCCAGA 
ATTGCCATTT CTAATTTACA TAAACAAACC ACAAAACAAT TTTCTAAGGT TGTCGAAGAT 
CTTTATAGAT ACGTCAATGC TGCTACTGGT AAGCCCGCTC CCATGATCTC TGATGATGTC 
TACAATATTG TCATGGAAAA CAAGGATAAA TTGAACTCCG CAATTGTCTA TGACAGAGAT 
TTTCAGTACA GTTATTTTGG TTTTAAAACT TTGGAACGTT CTTATTTACT AAGAATCAAC 
GGTCAAGTGG CCGAACGTCC ACAACATTTA ATTATGAGAG TCGCACTAGG CATCCACGGT 
AGAGATATCG AGGCTGCTTT AGAAACGTAT AACTTGATGT CTCTAAAATA TTTTACTCAC 
GCCTCTCCAA CGTTGTTCAA TGCCGGTACT CCAAAACCTC AAATGTCCTC TTGTTTCTTG 
GTTGCCATGA AGGAGGACTC TATCGAGGGG ATTTACGACA CCTTGAAGGA ATGTGCTTTG 
ATTTCCAAAA CTGCTGGTGG TATTGGTCTA CATATCCATA ACATTCGTTC AACTGGTTCT 
TACATTGCTG GTACAAACGG TACTTCTAAC GGTTTAATTC CTATGATTCG TGTTTTCAAT 
AACACTGCCC GTTATGTTGA CCAGGGTGGT AATAAAAGAC CTGGTGCGTT TGCCCTTTAC 
CTGGAACCAT GGCATGCTGA TATATTTGAT TTTATTGATA TTAGGAAGAA CCACGGTAAA 
GAGGAAATTC GTGCAAGAGA TTTGTTCCCT GCTCTATGGA TTCCTGATCT TTTCATGAAG 
CGTGTCGAAG AAAATGGGAC CTGGACATTA TTCTCTCCAA CATCAGCTCC TGGTTTAAGC 
GATTGTTACG GTGACGAGTT TGAGGCTCTA TATACCCGCT ACGAGAAAGA AGGTCGTGGT 
AAGACTATCA AAGCCCAAAA ATTATGGTAT TCCATTTTGG AAGCTCAAAC TGAAACTGGT 
ACACCTTTCG TTGTTTACAA GGATGCTTGT AACAGAAAAT CTAATCAAAA AAACTTAGGT 
GTCATCAAGT CATCAAACTT ATGCTGTGAA ATTGTTGAAT ACTCAGCTCC AGATGAAACT 
GCTGTTTGTA ACTTGGCTTC CGTTGCCTTA CCAGCATTCA TTGAAACTTC TGAGGATGGT 
AAGACTTCCA CATACAACTT CAAAAAATTA CATGAAATTG CTAAAGTTGT TACTCGTAAT 
TTAAACAGAG TCATTGATCG TAATTACTAC CCTGTTGAAG AAGCGAGAAA ATCCAATATG 
AGACATAGAC CAATTGCTTT GGGTGTTCAA GGTCTCGCTG ACACTTTCAT GCTGTTACGT 
TTGCCATTTG ATTCTGAGGA AGCCCGTTTG CTAAATATCC AAATCTTTGA AACTATTTAT 
CATGCCTCCA TGGAAGCTTC TTGTGAACTA GCTCAGAAGG ACGGTCCATA CGAAACTTTC 
CAAGGATCTC CTGCTTCTCA AGGTATACTA CAGTTTGATA TGTGGGACCA AAAACCCTAC 
GGCATGTGGG ATTGGGACAC CTTAAGAAAA GATATCATGA AGCATGGTGT TAGAAATTCC 
TTGACCATGG CACCAATGCC TACTGCATCC ACATCCCAAA TATTGGGTTA TAATGAATGT 
TTCGAACCAG TCACTTCCAA TATGTACTCC CGTCGTGTCT TATCCGGTGA ATTCCAGGTT 
GTGAACCCTT ACTTACTGCG TGACTTGGTT GATTTAGGTA TTTGGGATGA GGGTATGAAA 
CAGTATCTGA TTACACAAAA TGGCTCCATT CAAGGCTTAC CAAACGTTCC ACAAGAATTG 
AAGGACTTAT ACAAGACTGT TTGGGAAATT TCACAAAAGA CTATCATTAA CATGGCAGCC 
GATCGTTCTG TCTATATTGA TCAATCTCAT TCTTTGAATT TGTTCTTACG TGCCCCAACT 
ATGGGTAAAC TAACAAGTAT GCATTTTTAC GGATGGAAGA AGGGATTGAA GACCGGTATG 
TACTATTTGA GAACCCAAGC TGCATCTGCT GCAATTCAAT TTACTATTGA TCAGAAGATT 
GCGGATCAAG CTACAGAAAA CGTTGCTGAT ATTTCCAACT TGAAGCGTCC ATCATATATG 
CCTTCCAGTG CAAGCTACGC TGCCAGCGAT TTCGTGCCCG CAGCTGTGAC TGCAAACGCA 
ACTATTCCAT CTCTAGATAG CTCCTCGGAA GCTTCAAGAG AGGCATCTCC AGCTCCAACA 
GGTAGCCACT CATTAACTAA AGGAATGGCA GAATTAAACG TTCAAGAGTC TAAGGTAGAA 
GTTCCTGAAG TACCTGCCCC AACTAAGAAT GAAGAAAAAG CTGCCCCCAT CGTTGATGAT 
GAGGAAACCG AGTTCGACAT TTACAACTCT AAGGTTATAG CATGTGCTAT TGATAACCCA 
GAAGCTTGTG AAATGTGTTC GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

RNR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報