SET1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SET1
Entrez Gene ID	856519
Full Name	histone methyltransferase SET1
Synonyms	KMT2,YTX1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179249.1	NP_011987.1	histone methyltransferase SET1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	set1	NP_587812.1
Saccharomyces cerevisiae (baker's yeast)	SET1	NP_011987.1
Kluyveromyces lactis	KLLA0F24134g	XP_456155.1
Eremothecium gossypii	AGOS_ABR136W	NP_983083.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Set1 lacking RNA recognition motif 2 (RRM2) showed reduced in vivo cross-linking to RNA and reduced chromatin occupancy. In addition, levels of H3K4 trimethylation were decreased, whereas levels of dimethylation were increased
Title: RNA Binding by Histone Methyltransferases Set1 and Set2.

Set1 and Set5 promote gene silencing at telomeric regions. Double mutants Set1 and Set5 display defects in telomere stability.
Title: The histone methyltransferases Set5 and Set1 have overlapping functions in gene silencing and telomere maintenance.

Transcriptional memory of INO1 requires a remodeled form of the Set1/COMPASS methyltransferase lacking Spp1, which dimethylates histone H3 lysine 4 (H3K4me2).
Title: Set1/COMPASS and Mediator are repurposed to promote epigenetic transcriptional memory.

The study uncovers novel pathways in which the methyltransferase Set5 participates and, more importantly, reveals a partnership between Set1 and Set5 in transcriptional repression near repetitive DNA elements in budding yeast.
Title: Set5 and Set1 cooperate to repress gene expression at telomeres and retrotransposons.

Careful tuning of Set1 levels by regulated degradation is critical for the establishment and maintenance of proper H3K4 methylation patterns.
Title: Feedback control of Set1 protein levels is important for proper H3K4 methylation patterns.

The following SET1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SET1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05317	NM_001179249.1 Latest version!	Saccharomyces cerevisiae S288C histone methyltransferase SET1 (SET1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SET1 ( NM_001179249.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011987.1 Saccharomyces cerevisiae S288C histone methyltransferase SET1 (SET1), partial mRNA.

Price & Availability↑

CloneID	OSi05317
Clone ID Related Accession (Same CDS sequence)	NM_001179249.1
Accession Version	NM_001179249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	histone methyltransferase SET1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAAATT ACTATAGAAG AGCACACGCG TCTTCTGGTT CATACAGACA ACCCCAGGAA 
CAGCCTCAAT ATTCGCGTTC TGGTCACTAT CAGTATTCAA ACGGCCATTC TCACCAACAA 
TATTCTAGTC AATATAATCA ACGTCGACGT TATAACCATA ATGATGGTAC AAGGCGACGC 
TATAATGACG ATCGCCCACA TAGTTCAAAC AATGCAAGTA CGCGACAGTA CTATGCTACT 
AACAACAGCC AAAGCGGCCC ATATGTAAAT AAGAAATCTG ACATCAGTAG TCGGAGGGGC 
ATGTCTCAAT CACGGTATTC AAATAGCAAT GTTCACAATA CATTAGCGTC TTCGAGTGGA 
TCTCTTCCCA CAGAATCTGC TCTGCTTTTG CAACAAAGAC CACCTTCAGT TTTGAGATAC 
AACACAGATA ATTTGAAGTC TAAGTTTCAT TATTTTGATC CCATAAAAGG CGAGTTCTTC 
AATAAGGATA AGATGCTTTC GTGGAAGGCT ACAGATAAAG AATTTTCTGA AACAGGTTAT 
TACGTAGTCA AAGAGTTACA AGATGGACAG TTTAAGTTCA AAATAAAACA CAGACATCCG 
GAGATAAAAG CATCCGACCC ACGTAATGAA AACGGTATCA TGACTAGCGG AAAAGTGGCA 
ACCCACAGAA AATGCAGGAA CTCACTAATT CTATTGCCTC GCATATCTTA TGACAGGTAC 
TCCTTAGGGC CTCCCCCTTC ATGTGAAATA GTTGTCTATC CAGCGCAAGA TTCAACAACA 
ACCAATATCC AAGACATATC AATAAAAAAC TATTTTAAAA AGTATGGAGA AATTTCTCAT 
TTTGAAGCAT TTAATGATCC TAATAGCGCT TTACCTTTGC ATGTTTATCT TATAAAGTAT 
GCCAGTTCTG ATGGAAAAAT TAATGATGCA GCAAAAGCAG CCTTTAGTGC CGTTAGAAAG 
CACGAATCTT CGGGTTGCTT TATCATGGGC TTCAAGTTCG AAGTGATTTT AAACAAGCAT 
TCCATTTTGA ATAATATCAT TTCTAAATTT GTTGAAATAA ATGTCAAAAA GCTACAGAAG 
TTACAAGAGA ACCTGAAGAA GGCTAAAGAG AAAGAAGCAG AAAACGAAAA AGCAAAGGAA 
TTACAGGGCA AAGATATTAC CTTGCCCAAG GAACCTAAGG TAGACACATT ATCTCATTCG 
TCCGGAAGTG AAAAAAGAAT TCCATATGAT CTCTTGGGGG TAGTTAATAA CAGACCTGTT 
TTACATGTCT CCAAAATATT TGTTGCCAAA CATAGGTTCT GCGTTGAGGA CTTTAAATAC 
AAGTTAAGGG GATACAGATG TGCGAAATTT ATTGATCATC CAACTGGTAT CTATATTATT 
TTTAATGACA TTGCCCATGC GCAAACATGT TCGAATGCAG AGTCAGGAAA TTTAACAATA 
ATGTCTCGGA GCAGAAGAAT TCCTATTCTA ATAAAGTTTC ATCTCATTCT CCCTAGGTTC 
CAAAACAGAA CTAGATTCAA TAAATCTAGC TCATCTTCAA ATTCTACAAA TGTACCTATA 
AAATACGAGT CCAAAGAGGA GTTCATTGAA GCTACAGCAA AACAAATATT AAAAGATTTG 
GAAAAGACTT TACATGTTGA TATTAAGAAG AGATTGATTG GTCCTACGGT ATTTGATGCT 
TTGGACCATG CAAATTTTCC TGAATTGTTA GCTAAAAGAG AACTAAAGGA GAAAGAGAAG 
AGACAACAGA TTGCATCTAA AATTGCTGAA GATGAATTGA AACGTAAAGA AGAAGCCAAA 
AGAGATTTTG ATTTGTTTGG TTTATATGGT GGCTATGCAA AATCTAATAA AAGAAATTTA 
AAAAGGCATA ATTCACTCGC GTTGGATCAT ACTTCTTTAA AGAGGAAAAA GCTATCCAAT 
GGTATCAAAC CAATGGCACA TTTACTGAAC GAAGAAACCG ATTCCAAAGA AACTACCCCA 
TTGAACGATG AAGGGATCAC TCGCGTATCA AAAGAACATG ATGAAGAAGA CGAAAATATG 
ACATCTTCAT CTTCTGAAGA AGAGGAAGAA GAAGCTCCAG ATAAGAAATT CAAGAGTGAG 
TCTGAGCCAA CCACCCCCGA ATCTGATCAC CTTCATGGTA TTAAGCCGTT AGTACCCGAT 
CAAAATGGGT CGTCTGACGT ACTGGATGCT TCTTCGATGT ATAAACCTAC TGCTACCGAA 
ATTCCCGAAC CTGTATATCC ACCTGAGGAA TATGACTTGA AATATAGTCA GACTTTATCT 
TCTATGGATT TGCAGAATGC TATCAAAGAT GAGGAAGATA TGCTAATTTT AAAGCAGTTA 
TTGAGCACAT ATACTCCTAC CGTCACACCA GAAACAAGCG CAGCTCTGGA ATATAAAATT 
TGGCAATCTC GCCGAAAAGT TCTTGAAGAA GAGAAGGCTT CCGATTGGCA AATAGAGCTT 
AATGGAACTT TATTTGATAG TGAACTACAA CCAGGTAGCT CTTTTAAAGC TGAAGGGTTC 
AGGAAAATTG CGGATAAATT AAAAATTAAT TACCTACCCC ATCGTCGCAG AGTTCACCAA 
CCTTTAAATA CGGTGAATAT TCACAATGAA AGGAATGAGT ACACACCTGA ACTTTGTCAA 
AGAGAAGAAT CCTCGAATAA AGAACCTTCA GACTCAGTTC CTCAAGAAGT TTCATCCTCT 
AGAGATAATA GGGCATCAAA TAGAAGATTT CAGCAGGACA TAGAGGCACA GAAAGCCGCA 
ATTGGTACGG AATCTGAGCT GCTATCACTA AATCAATTAA ATAAAAGAAA AAAGCCAGTT 
ATGTTCGCTC GTTCAGCAAT TCACAACTGG GGTTTATATG CTCTAGACTC TATCGCAGCA 
AAGGAAATGA TTATCGAGTA CGTTGGTGAA AGGATCAGGC AACCTGTAGC AGAAATGAGA 
GAGAAAAGAT ATCTGAAAAA TGGGATTGGA TCCAGTTACC TTTTTAGGGT TGATGAAAAC 
ACGGTTATTG ATGCCACCAA GAAAGGTGGT ATAGCCCGTT TCATTAATCA TTGTTGTGAT 
CCAAATTGTA CGGCAAAGAT TATAAAGGTT GGCGGGAGAA GGAGAATTGT TATCTATGCA 
CTGCGTGATA TCGCGGCAAG CGAAGAGTTG ACATATGATT ACAAATTTGA GAGAGAAAAG 
GATGACGAGG AAAGACTTCC TTGTTTATGT GGAGCACCTA ATTGTAAAGG TTTCTTGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011987.1
CDS	1..3243
Translation	MSNYYRRAHASSGSYRQPQEQPQYSRSGHYQYSNGHSHQQYSSQYNQRRRYNHNDGTRRRYNDDRPHSSNNASTRQYYATNNSQSGPYVNKKSDISSRRGMSQSRYSNSNVHNTLASSSGSLPTESALLLQQRPPSVLRYNTDNLKSKFHYFDPIKGEFFNKDKMLSWKATDKEFSETGYYVVKELQDGQFKFKIKHRHPEIKASDPRNENGIMTSGKVATHRKCRNSLILLPRISYDRYSLGPPPSCEIVVYPAQDSTTTNIQDISIKNYFKKYGEISHFEAFNDPNSALPLHVYLIKYASSDGKINDAAKAAFSAVRKHESSGCFIMGFKFEVILNKHSILNNIISKFVEINVKKLQKLQENLKKAKEKEAENEKAKELQGKDITLPKEPKVDTLSHSSGSEKRIPYDLLGVVNNRPVLHVSKIFVAKHRFCVEDFKYKLRGYRCAKFIDHPTGIYIIFNDIAHAQTCSNAESGNLTIMSRSRRIPILIKFHLILPRFQNRTRFNKSSSSSNSTNVPIKYESKEEFIEATAKQILKDLEKTLHVDIKKRLIGPTVFDALDHANFPELLAKRELKEKEKRQQIASKIAEDELKRKEEAKRDFDLFGLYGGYAKSNKRNLKRHNSLALDHTSLKRKKLSNGIKPMAHLLNEETDSKETTPLNDEGITRVSKEHDEEDENMTSSSSEEEEEEAPDKKFKSESEPTTPESDHLHGIKPLVPDQNGSSDVLDASSMYKPTATEIPEPVYPPEEYDLKYSQTLSSMDLQNAIKDEEDMLILKQLLSTYTPTVTPETSAALEYKIWQSRRKVLEEEKASDWQIELNGTLFDSELQPGSSFKAEGFRKIADKLKINYLPHRRRVHQPLNTVNIHNERNEYTPELCQREESSNKEPSDSVPQEVSSSRDNRASNRRFQQDIEAQKAAIGTESELLSLNQLNKRKKPVMFARSAIHNWGLYALDSIAAKEMIIEYVGERIRQPVAEMREKRYLKNGIGSSYLFRVDENTVIDATKKGGIARFINHCCDPNCTAKIIKVGGRRRIVIYALRDIAASEELTYDYKFEREKDDEERLPCLCGAPNCKGFLN

Target ORF information:

RefSeq Version	NM_001179249.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C histone methyltransferase SET1 (SET1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179249.1

ORF Insert Sequence:

ATGTCAAATT ACTATAGAAG AGCACACGCG TCTTCTGGTT CATACAGACA ACCCCAGGAA 
CAGCCTCAAT ATTCGCGTTC TGGTCACTAT CAGTATTCAA ACGGCCATTC TCACCAACAA 
TATTCTAGTC AATATAATCA ACGTCGACGT TATAACCATA ATGATGGTAC AAGGCGACGC 
TATAATGACG ATCGCCCACA TAGTTCAAAC AATGCAAGTA CGCGACAGTA CTATGCTACT 
AACAACAGCC AAAGCGGCCC ATATGTAAAT AAGAAATCTG ACATCAGTAG TCGGAGGGGC 
ATGTCTCAAT CACGGTATTC AAATAGCAAT GTTCACAATA CATTAGCGTC TTCGAGTGGA 
TCTCTTCCCA CAGAATCTGC TCTGCTTTTG CAACAAAGAC CACCTTCAGT TTTGAGATAC 
AACACAGATA ATTTGAAGTC TAAGTTTCAT TATTTTGATC CCATAAAAGG CGAGTTCTTC 
AATAAGGATA AGATGCTTTC GTGGAAGGCT ACAGATAAAG AATTTTCTGA AACAGGTTAT 
TACGTAGTCA AAGAGTTACA AGATGGACAG TTTAAGTTCA AAATAAAACA CAGACATCCG 
GAGATAAAAG CATCCGACCC ACGTAATGAA AACGGTATCA TGACTAGCGG AAAAGTGGCA 
ACCCACAGAA AATGCAGGAA CTCACTAATT CTATTGCCTC GCATATCTTA TGACAGGTAC 
TCCTTAGGGC CTCCCCCTTC ATGTGAAATA GTTGTCTATC CAGCGCAAGA TTCAACAACA 
ACCAATATCC AAGACATATC AATAAAAAAC TATTTTAAAA AGTATGGAGA AATTTCTCAT 
TTTGAAGCAT TTAATGATCC TAATAGCGCT TTACCTTTGC ATGTTTATCT TATAAAGTAT 
GCCAGTTCTG ATGGAAAAAT TAATGATGCA GCAAAAGCAG CCTTTAGTGC CGTTAGAAAG 
CACGAATCTT CGGGTTGCTT TATCATGGGC TTCAAGTTCG AAGTGATTTT AAACAAGCAT 
TCCATTTTGA ATAATATCAT TTCTAAATTT GTTGAAATAA ATGTCAAAAA GCTACAGAAG 
TTACAAGAGA ACCTGAAGAA GGCTAAAGAG AAAGAAGCAG AAAACGAAAA AGCAAAGGAA 
TTACAGGGCA AAGATATTAC CTTGCCCAAG GAACCTAAGG TAGACACATT ATCTCATTCG 
TCCGGAAGTG AAAAAAGAAT TCCATATGAT CTCTTGGGGG TAGTTAATAA CAGACCTGTT 
TTACATGTCT CCAAAATATT TGTTGCCAAA CATAGGTTCT GCGTTGAGGA CTTTAAATAC 
AAGTTAAGGG GATACAGATG TGCGAAATTT ATTGATCATC CAACTGGTAT CTATATTATT 
TTTAATGACA TTGCCCATGC GCAAACATGT TCGAATGCAG AGTCAGGAAA TTTAACAATA 
ATGTCTCGGA GCAGAAGAAT TCCTATTCTA ATAAAGTTTC ATCTCATTCT CCCTAGGTTC 
CAAAACAGAA CTAGATTCAA TAAATCTAGC TCATCTTCAA ATTCTACAAA TGTACCTATA 
AAATACGAGT CCAAAGAGGA GTTCATTGAA GCTACAGCAA AACAAATATT AAAAGATTTG 
GAAAAGACTT TACATGTTGA TATTAAGAAG AGATTGATTG GTCCTACGGT ATTTGATGCT 
TTGGACCATG CAAATTTTCC TGAATTGTTA GCTAAAAGAG AACTAAAGGA GAAAGAGAAG 
AGACAACAGA TTGCATCTAA AATTGCTGAA GATGAATTGA AACGTAAAGA AGAAGCCAAA 
AGAGATTTTG ATTTGTTTGG TTTATATGGT GGCTATGCAA AATCTAATAA AAGAAATTTA 
AAAAGGCATA ATTCACTCGC GTTGGATCAT ACTTCTTTAA AGAGGAAAAA GCTATCCAAT 
GGTATCAAAC CAATGGCACA TTTACTGAAC GAAGAAACCG ATTCCAAAGA AACTACCCCA 
TTGAACGATG AAGGGATCAC TCGCGTATCA AAAGAACATG ATGAAGAAGA CGAAAATATG 
ACATCTTCAT CTTCTGAAGA AGAGGAAGAA GAAGCTCCAG ATAAGAAATT CAAGAGTGAG 
TCTGAGCCAA CCACCCCCGA ATCTGATCAC CTTCATGGTA TTAAGCCGTT AGTACCCGAT 
CAAAATGGGT CGTCTGACGT ACTGGATGCT TCTTCGATGT ATAAACCTAC TGCTACCGAA 
ATTCCCGAAC CTGTATATCC ACCTGAGGAA TATGACTTGA AATATAGTCA GACTTTATCT 
TCTATGGATT TGCAGAATGC TATCAAAGAT GAGGAAGATA TGCTAATTTT AAAGCAGTTA 
TTGAGCACAT ATACTCCTAC CGTCACACCA GAAACAAGCG CAGCTCTGGA ATATAAAATT 
TGGCAATCTC GCCGAAAAGT TCTTGAAGAA GAGAAGGCTT CCGATTGGCA AATAGAGCTT 
AATGGAACTT TATTTGATAG TGAACTACAA CCAGGTAGCT CTTTTAAAGC TGAAGGGTTC 
AGGAAAATTG CGGATAAATT AAAAATTAAT TACCTACCCC ATCGTCGCAG AGTTCACCAA 
CCTTTAAATA CGGTGAATAT TCACAATGAA AGGAATGAGT ACACACCTGA ACTTTGTCAA 
AGAGAAGAAT CCTCGAATAA AGAACCTTCA GACTCAGTTC CTCAAGAAGT TTCATCCTCT 
AGAGATAATA GGGCATCAAA TAGAAGATTT CAGCAGGACA TAGAGGCACA GAAAGCCGCA 
ATTGGTACGG AATCTGAGCT GCTATCACTA AATCAATTAA ATAAAAGAAA AAAGCCAGTT 
ATGTTCGCTC GTTCAGCAAT TCACAACTGG GGTTTATATG CTCTAGACTC TATCGCAGCA 
AAGGAAATGA TTATCGAGTA CGTTGGTGAA AGGATCAGGC AACCTGTAGC AGAAATGAGA 
GAGAAAAGAT ATCTGAAAAA TGGGATTGGA TCCAGTTACC TTTTTAGGGT TGATGAAAAC 
ACGGTTATTG ATGCCACCAA GAAAGGTGGT ATAGCCCGTT TCATTAATCA TTGTTGTGAT 
CCAAATTGTA CGGCAAAGAT TATAAAGGTT GGCGGGAGAA GGAGAATTGT TATCTATGCA 
CTGCGTGATA TCGCGGCAAG CGAAGAGTTG ACATATGATT ACAAATTTGA GAGAGAAAAG 
GATGACGAGG AAAGACTTCC TTGTTTATGT GGAGCACCTA ATTGTAAAGG TTTCTTGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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SET1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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