ORF » Species Summary » Saccharomyces cerevisiae S288C » BIG1 cDNA ORF clone

BIG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

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  • Clones

  • gRNAs

The following BIG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BIG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi05299 NM_001179231.1
Latest version!		 Saccharomyces cerevisiae S288C Big1p (BIG1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    BIG1 ( NM_001179231.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011969.1 Saccharomyces cerevisiae S288C Big1p (BIG1), partial mRNA.
    Price & Availability↑
    CloneID OSi05299
    Clone ID Related Accession (Same CDS sequence) NM_001179231.1
    Accession Version NM_001179231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Big1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAAACTG TGTTGAAATA TTTGTTGCTT ATTATGTGTG GAAGCTTTTG TGCTTCGGAG 
    GAATTGCAAA ACCAGACCAA TGTTCCTGCA ATATTCTTCT CTTACAAACT AACTCCCGGT 
    ATTTTAAAGT ATCAAGAGGA TTATGATAGG GCTGTGACTT TACCAAGAGA CACCTTTATT 
    GAAGCGGCTG AGAAATTCTT AGGTGTATGT AATGCTGACA CTTATGTGTT TATTAATCAA 
    CCAGGCCTGA GGAAATTGGA CTTCTTGGAA TTTGAAACAG AGTTTGTTTC GTTACAGAGA 
    TATATAAGAC GGAGTTCAAC CGCGATTAAG TTCGAAAAGG TAGACCTATT GCCTCAAGAT 
    TTATACTATG ATCTTGCAGA GTTTGTAAAG GAGTATTGTA ACGTAGACCA GGTGTTGAAC 
    TTACGAGGTA ATAATACAGA AGATTTCCAG CCCTTTATTG ATAGCGAAAA AAGAGTGATT 
    ATAATAGAGT ACCCTAAGTT ACCAGAGGAC ACTAATGAAC GTAAAGAAGC ATTCCGCCAT 
    TATGACAAGT ATTTGAGGAC TATACTGGCG CAGATTCCGT CGCCAGAACA AAATGTCATT 
    TACACCTCGT TAAACCCAGG AACTACGCTG GCTCACGAGT CGATCATCCC TATCCAAATA 
    TTTCCAGATA TCTTTGACAT AAAATCCAGA GTGGGTGAAG TGGAACAAAA TAATAGAGTT 
    TTGGATGTGC CTCGTCTGTC GTTTAATGAC TATACTCCAC GATTTTCAGA GCCACCTTCG 
    GAGTATGTCT CAATATTTGA TTCACAATTA ATTGAGAATA ACAGAGGTTT ATTACAGCTG 
    ATTTTCACAA TTTTGGTTGG GTATATTCTT ATTCAGTTTT TTTTCACAAA AAAAACTATT 
    GTAGATGAGA AGATTACTAA TAAAAAGGAC AACGTGAAAC AGACGTCACC ACAACTGCTC 
    AAAAAAGTGC AAGAGATCCA GAAAAAGCCA TCCCAGCAAG TATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011969.1
    CDS1..1008
    Translation

    Target ORF information:

    RefSeq Version NM_001179231.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Big1p (BIG1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001179231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAAACTG TGTTGAAATA TTTGTTGCTT ATTATGTGTG GAAGCTTTTG TGCTTCGGAG 
    GAATTGCAAA ACCAGACCAA TGTTCCTGCA ATATTCTTCT CTTACAAACT AACTCCCGGT 
    ATTTTAAAGT ATCAAGAGGA TTATGATAGG GCTGTGACTT TACCAAGAGA CACCTTTATT 
    GAAGCGGCTG AGAAATTCTT AGGTGTATGT AATGCTGACA CTTATGTGTT TATTAATCAA 
    CCAGGCCTGA GGAAATTGGA CTTCTTGGAA TTTGAAACAG AGTTTGTTTC GTTACAGAGA 
    TATATAAGAC GGAGTTCAAC CGCGATTAAG TTCGAAAAGG TAGACCTATT GCCTCAAGAT 
    TTATACTATG ATCTTGCAGA GTTTGTAAAG GAGTATTGTA ACGTAGACCA GGTGTTGAAC 
    TTACGAGGTA ATAATACAGA AGATTTCCAG CCCTTTATTG ATAGCGAAAA AAGAGTGATT 
    ATAATAGAGT ACCCTAAGTT ACCAGAGGAC ACTAATGAAC GTAAAGAAGC ATTCCGCCAT 
    TATGACAAGT ATTTGAGGAC TATACTGGCG CAGATTCCGT CGCCAGAACA AAATGTCATT 
    TACACCTCGT TAAACCCAGG AACTACGCTG GCTCACGAGT CGATCATCCC TATCCAAATA 
    TTTCCAGATA TCTTTGACAT AAAATCCAGA GTGGGTGAAG TGGAACAAAA TAATAGAGTT 
    TTGGATGTGC CTCGTCTGTC GTTTAATGAC TATACTCCAC GATTTTCAGA GCCACCTTCG 
    GAGTATGTCT CAATATTTGA TTCACAATTA ATTGAGAATA ACAGAGGTTT ATTACAGCTG 
    ATTTTCACAA TTTTGGTTGG GTATATTCTT ATTCAGTTTT TTTTCACAAA AAAAACTATT 
    GTAGATGAGA AGATTACTAA TAAAAAGGAC AACGTGAAAC AGACGTCACC ACAACTGCTC 
    AAAAAAGTGC AAGAGATCCA GAAAAAGCCA TCCCAGCAAG TATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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