SRO7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SRO7
Entrez Gene ID	856144
Full Name	Rab GTPase-binding protein SRO7
Synonyms	SNI1,SOP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184129.1	NP_015357.1	Rab GTPase-binding protein SRO7

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SRO77	NP_009444.2
Saccharomyces cerevisiae (baker's yeast)	SRO7	NP_015357.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors describe the identification of a Sec4 binding site on the surface of Sro7 that is contained within a cleft created by the junction of two adjacent beta-propellers that form the core structure of Sro7.
Title: Structural basis for recognition of the Sec4 Rab GTPase by its effector, the Lgl/tomosyn homologue, Sro7.

Intracellular traffic in yeast between the Golgi and the cell surface is mediated by vesicular carriers and highly dependent on both Sro7 and Rab GTPase, Sec4.
Title: In vitro reconstitution of Rab GTPase-dependent vesicle clustering by the yeast lethal giant larvae/tomosyn homolog, Sro7.

These results suggested that the tumour suppressor homologue SRO7/SRO77 regulated cell proliferation and yeast colony development via the Rho1-Tor1 pathway.
Title: Sro7 and Sro77, the yeast homologues of the Drosophila lethal giant larvae (Lgl), regulate cell proliferation via the Rho1-Tor1 pathway.

Data show that vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins.
Title: Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion.

Results characterize the interaction between Sro7 and the type V myosin Myo2 and show that this interaction is important for two distinct aspects of Sro7 function.
Title: Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis.

The following SRO7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRO7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04993	NM_001184129.1 Latest version!	Saccharomyces cerevisiae S288C Rab GTPase-binding protein SRO7 (SRO7), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SRO7 ( NM_001184129.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015357.1 Saccharomyces cerevisiae S288C Rab GTPase-binding protein SRO7 (SRO7), partial mRNA.

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CloneID	OSi04993
Clone ID Related Accession (Same CDS sequence)	NM_001184129.1
Accession Version	NM_001184129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rab GTPase-binding protein SRO7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTTCGGTA GCAAACGTCT GAAAAATGTT AAGGAAGCAT TTAAGTCCCT AAAAGGCCAA 
AATTCAGAGA CACCCATTGA GAACTCAAAG GCGTCTTTTA AATCCAAAAA TTCAAAGACA 
TCTACTATTT CCAAAGATGC GAAATCTTCC TCTTCTTTGA AGATTCCTAT AAGTTCAAAT 
AACAAAAATA AAATTTTTTC ACTAGCAGAA ACCAACAAAT ATGGGATGAG CAGTAAACCG 
ATCGCAGCCG CTTTCGATTT CACCCAAAAT TTATTGGCAA TTGCTACAGT CACAGGGGAA 
GTCCATATTT ATGGCCAGCA ACAAGTGGAA GTGGTTATAA AACTGGAGGA TAGATCTGCT 
ATAAAAGAAA TGAGATTTGT GAAAGGTATC TATTTGGTGG TCATTAACGC AAAAGATACT 
GTGTATGTTT TATCGTTATA TTCTCAAAAA GTACTTACAA CAGTGTTTGT TCCGGGGAAA 
ATAACATCAA TTGACACAGA TGCATCCTTA GACTGGATGC TAATCGGCCT TCAAAATGGT 
TCAATGATAG TTTACGACAT AGATAGGGAT CAACTTTCCT CCTTCAAATT AGACAACCTA 
CAAAAAAGCT CATTTTTTCC GGCTGCAAGA CTATCACCTA TAGTATCCAT TCAGTGGAAC 
CCTAGAGATA TTGGAACTGT TTTAATATCA TATGAGTATG TAACTCTAAC ATACTCATTG 
GTTGAGAACG AAATCAAACA ATCCTTCATA TACGAATTAC CACCTTTTGC TCCAGGAGGC 
GACTTCTCAG AAAAAACCAA TGAAAAGAGA ACTCCCAAAG TTATTCAGTC CTTATATCAC 
CCTAATTCAC TGCACATTAT CACTATTCAT GAAGACAATT CCTTGGTTTT CTGGGATGCT 
AATTCAGGCC ATATGATCAT GGCAAGGACA GTGTTTGAGA CAGAAATCAA TGTTCCACAA 
CCTGATTACA TACGCGATTC TAGTACTAAT GCTGCCAAAA TTTCGAAAGT GTATTGGATG 
TGTGAAAACA ATCCAGAATA TACTTCACTA CTCATCTCTC ATAAGTCAAT CAGCAGAGGA 
GATAATCAAA GTCTCACTAT GATCGACCTC GGTTACACAC CTAGATATTC AATAACCTCT 
TATGAAGGTA TGAAGAACTA CTATGCAAAT CCGAAACAAA TGAAGATATT TCCCTTACCA 
ACCAATGTGC CAATAGTGAA TATACTACCA ATTCCAAGAC AGTCCCCGTA TTTTGCAGGA 
TGTCATAATC CGGGGTTAAT TTTGTTAATC CTAGGTAACG GTGAGATAGA AACAATGCTA 
TATCCTTCTG GAATATTTAC AGATAAAGCC TCTTTATTCC CTCAAAATCT TTCCTGGCTG 
AGACCATTAG CCACGACTTC AATGGCCGCT TCTGTACCAA ACAAATTATG GCTTGGTGCG 
CTTTCTGCAG CTCAAAACAA GGATTATTTG TTGAAGGGAG GGGTTAGAAC AAAAAGGCAA 
AAACTTCCCG CTGAGTACGG TACAGCATTC ATTACCGGCC ATTCTAATGG CTCAGTTAGG 
ATATACGATG CTTCCCATGG GGATATACAA GATAATGCTA GCTTTGAAGT GAATCTATCA 
AGAACGTTGA ACAAGGCCAA AGAACTAGCA GTTGATAAGA TATCTTTTGC CGCAGAGACT 
CTCGAGTTGG CGGTGTCAAT TGAAACAGGA GACGTTGTTT TATTCAAATA TGAAGTAAAC 
CAGTTTTACA GTGTTGAGAA CAGACCAGAA AGCGGTGACC TGGAGATGAA TTTCAGGCGG 
TTTTCTTTGA ATAATACGAA TGGTGTTCTC GTCGATGTGA GAGACAGGGC ACCTACCGGT 
GTTAGGCAGG GATTTATGCC ATCAACTGCC GTGCATGCCA ACAAAGGGAA AACATCTGCA 
ATCAACAACA GTAATATTGG ATTTGTTGGA ATCGCATATG CTGCCGGAAG CCTTATGTTG 
ATCGATAGAA GAGGTCCTGC AATCATTTAC ATGGAAAACA TTAGAGAAAT CTCGGGAGCT 
CAAAGCGCAT GTGTTACCTG CATCGAATTC GTAATTATGG AGTATGGAGA CGATGGTTAC 
TCTAGTATAT TGATGGTCTG TGGTACTGAT ATGGGCGAGG TAATCACATA CAAAATTCTT 
CCTGCATCAG GTGGAAAGTT CGATGTGCAA CTAATGGATA TTACTAATGT TACCAGTAAG 
GGTCCGATAC ACAAAATTGA TGCATTTTCA AAGGAAACAA AATCTAGCTG CTTGGCAACT 
ATTCCAAAAA TGCAGAATTT AAGTAAAGGC CTTTGCATTC CTGGTATTGT ATTAATAACT 
GGATTCGATG ATATCAGATT AATAACACTG GGCAAATCTA AAAGTACACA TAAAGGTTTC 
AAATACCCGC TTGCCGCGAC AGGTTTATCT TACATCTCAA CCGTTGAGAA AAATAATGAT 
AGGAAGAACC TAACCGTAAT AATTACATTA GAAATCAATG GCCATCTAAG AGTTTTTACT 
ATTCCAGATT TCAAAGAACA AATGTCTGAA CATATTCCAT TTCCAATTGC CGCAAAATAC 
ATTACAGAAT CATCTGTTTT AAGAAATGGT GATATAGCGA TTCGAGTGAG TGAATTTCAG 
GCGTCACTAT TTTCTACTGT CAAGGAACAG GATACGCTAG CGCCAGTTTC TGACACACTG 
TACATCAATG GAATCAGGAT TCCCTATAGG CCACAAGTAA ATTCATTACA GTGGGCAAGA 
GGCACTGTAT ATTGTACTCC CGCTCAATTA AATGAATTAT TGGGTGGCGT GAATAGACCT 
GCATCCAAAT ACAAAGAGAG CATCATTGCT GAGGGAAGTT TCTCGGAGCG TTCTTCAGAC 
GATAATAATG CGAACCATCC GGAACACCAG TATACCAAGC CCACCCGTAA GGGCAGGAAC 
AGTAGTTACG GGGTACTAAG AAATGTTTCT CGAGCTGTAG AAACTAGATG GGACGCCGTT 
GAAGATAGAT TCAACGACTA TGCTACCGCC ATGGGAGAAA CAATGAACGA GGCTGTTGAG 
CAGACTGGAA AAGATGTAAT GAAAGGTGCC CTTGGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015357.1
CDS	1..3102
Translation	MFGSKRLKNVKEAFKSLKGQNSETPIENSKASFKSKNSKTSTISKDAKSSSSLKIPISSNNKNKIFSLAETNKYGMSSKPIAAAFDFTQNLLAIATVTGEVHIYGQQQVEVVIKLEDRSAIKEMRFVKGIYLVVINAKDTVYVLSLYSQKVLTTVFVPGKITSIDTDASLDWMLIGLQNGSMIVYDIDRDQLSSFKLDNLQKSSFFPAARLSPIVSIQWNPRDIGTVLISYEYVTLTYSLVENEIKQSFIYELPPFAPGGDFSEKTNEKRTPKVIQSLYHPNSLHIITIHEDNSLVFWDANSGHMIMARTVFETEINVPQPDYIRDSSTNAAKISKVYWMCENNPEYTSLLISHKSISRGDNQSLTMIDLGYTPRYSITSYEGMKNYYANPKQMKIFPLPTNVPIVNILPIPRQSPYFAGCHNPGLILLILGNGEIETMLYPSGIFTDKASLFPQNLSWLRPLATTSMAASVPNKLWLGALSAAQNKDYLLKGGVRTKRQKLPAEYGTAFITGHSNGSVRIYDASHGDIQDNASFEVNLSRTLNKAKELAVDKISFAAETLELAVSIETGDVVLFKYEVNQFYSVENRPESGDLEMNFRRFSLNNTNGVLVDVRDRAPTGVRQGFMPSTAVHANKGKTSAINNSNIGFVGIAYAAGSLMLIDRRGPAIIYMENIREISGAQSACVTCIEFVIMEYGDDGYSSILMVCGTDMGEVITYKILPASGGKFDVQLMDITNVTSKGPIHKIDAFSKETKSSCLATIPKMQNLSKGLCIPGIVLITGFDDIRLITLGKSKSTHKGFKYPLAATGLSYISTVEKNNDRKNLTVIITLEINGHLRVFTIPDFKEQMSEHIPFPIAAKYITESSVLRNGDIAIRVSEFQASLFSTVKEQDTLAPVSDTLYINGIRIPYRPQVNSLQWARGTVYCTPAQLNELLGGVNRPASKYKESIIAEGSFSERSSDDNNANHPEHQYTKPTRKGRNSSYGVLRNVSRAVETRWDAVEDRFNDYATAMGETMNEAVEQTGKDVMKGALGF

Target ORF information:

RefSeq Version	NM_001184129.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rab GTPase-binding protein SRO7 (SRO7), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184129.1

ORF Insert Sequence:

ATGTTCGGTA GCAAACGTCT GAAAAATGTT AAGGAAGCAT TTAAGTCCCT AAAAGGCCAA 
AATTCAGAGA CACCCATTGA GAACTCAAAG GCGTCTTTTA AATCCAAAAA TTCAAAGACA 
TCTACTATTT CCAAAGATGC GAAATCTTCC TCTTCTTTGA AGATTCCTAT AAGTTCAAAT 
AACAAAAATA AAATTTTTTC ACTAGCAGAA ACCAACAAAT ATGGGATGAG CAGTAAACCG 
ATCGCAGCCG CTTTCGATTT CACCCAAAAT TTATTGGCAA TTGCTACAGT CACAGGGGAA 
GTCCATATTT ATGGCCAGCA ACAAGTGGAA GTGGTTATAA AACTGGAGGA TAGATCTGCT 
ATAAAAGAAA TGAGATTTGT GAAAGGTATC TATTTGGTGG TCATTAACGC AAAAGATACT 
GTGTATGTTT TATCGTTATA TTCTCAAAAA GTACTTACAA CAGTGTTTGT TCCGGGGAAA 
ATAACATCAA TTGACACAGA TGCATCCTTA GACTGGATGC TAATCGGCCT TCAAAATGGT 
TCAATGATAG TTTACGACAT AGATAGGGAT CAACTTTCCT CCTTCAAATT AGACAACCTA 
CAAAAAAGCT CATTTTTTCC GGCTGCAAGA CTATCACCTA TAGTATCCAT TCAGTGGAAC 
CCTAGAGATA TTGGAACTGT TTTAATATCA TATGAGTATG TAACTCTAAC ATACTCATTG 
GTTGAGAACG AAATCAAACA ATCCTTCATA TACGAATTAC CACCTTTTGC TCCAGGAGGC 
GACTTCTCAG AAAAAACCAA TGAAAAGAGA ACTCCCAAAG TTATTCAGTC CTTATATCAC 
CCTAATTCAC TGCACATTAT CACTATTCAT GAAGACAATT CCTTGGTTTT CTGGGATGCT 
AATTCAGGCC ATATGATCAT GGCAAGGACA GTGTTTGAGA CAGAAATCAA TGTTCCACAA 
CCTGATTACA TACGCGATTC TAGTACTAAT GCTGCCAAAA TTTCGAAAGT GTATTGGATG 
TGTGAAAACA ATCCAGAATA TACTTCACTA CTCATCTCTC ATAAGTCAAT CAGCAGAGGA 
GATAATCAAA GTCTCACTAT GATCGACCTC GGTTACACAC CTAGATATTC AATAACCTCT 
TATGAAGGTA TGAAGAACTA CTATGCAAAT CCGAAACAAA TGAAGATATT TCCCTTACCA 
ACCAATGTGC CAATAGTGAA TATACTACCA ATTCCAAGAC AGTCCCCGTA TTTTGCAGGA 
TGTCATAATC CGGGGTTAAT TTTGTTAATC CTAGGTAACG GTGAGATAGA AACAATGCTA 
TATCCTTCTG GAATATTTAC AGATAAAGCC TCTTTATTCC CTCAAAATCT TTCCTGGCTG 
AGACCATTAG CCACGACTTC AATGGCCGCT TCTGTACCAA ACAAATTATG GCTTGGTGCG 
CTTTCTGCAG CTCAAAACAA GGATTATTTG TTGAAGGGAG GGGTTAGAAC AAAAAGGCAA 
AAACTTCCCG CTGAGTACGG TACAGCATTC ATTACCGGCC ATTCTAATGG CTCAGTTAGG 
ATATACGATG CTTCCCATGG GGATATACAA GATAATGCTA GCTTTGAAGT GAATCTATCA 
AGAACGTTGA ACAAGGCCAA AGAACTAGCA GTTGATAAGA TATCTTTTGC CGCAGAGACT 
CTCGAGTTGG CGGTGTCAAT TGAAACAGGA GACGTTGTTT TATTCAAATA TGAAGTAAAC 
CAGTTTTACA GTGTTGAGAA CAGACCAGAA AGCGGTGACC TGGAGATGAA TTTCAGGCGG 
TTTTCTTTGA ATAATACGAA TGGTGTTCTC GTCGATGTGA GAGACAGGGC ACCTACCGGT 
GTTAGGCAGG GATTTATGCC ATCAACTGCC GTGCATGCCA ACAAAGGGAA AACATCTGCA 
ATCAACAACA GTAATATTGG ATTTGTTGGA ATCGCATATG CTGCCGGAAG CCTTATGTTG 
ATCGATAGAA GAGGTCCTGC AATCATTTAC ATGGAAAACA TTAGAGAAAT CTCGGGAGCT 
CAAAGCGCAT GTGTTACCTG CATCGAATTC GTAATTATGG AGTATGGAGA CGATGGTTAC 
TCTAGTATAT TGATGGTCTG TGGTACTGAT ATGGGCGAGG TAATCACATA CAAAATTCTT 
CCTGCATCAG GTGGAAAGTT CGATGTGCAA CTAATGGATA TTACTAATGT TACCAGTAAG 
GGTCCGATAC ACAAAATTGA TGCATTTTCA AAGGAAACAA AATCTAGCTG CTTGGCAACT 
ATTCCAAAAA TGCAGAATTT AAGTAAAGGC CTTTGCATTC CTGGTATTGT ATTAATAACT 
GGATTCGATG ATATCAGATT AATAACACTG GGCAAATCTA AAAGTACACA TAAAGGTTTC 
AAATACCCGC TTGCCGCGAC AGGTTTATCT TACATCTCAA CCGTTGAGAA AAATAATGAT 
AGGAAGAACC TAACCGTAAT AATTACATTA GAAATCAATG GCCATCTAAG AGTTTTTACT 
ATTCCAGATT TCAAAGAACA AATGTCTGAA CATATTCCAT TTCCAATTGC CGCAAAATAC 
ATTACAGAAT CATCTGTTTT AAGAAATGGT GATATAGCGA TTCGAGTGAG TGAATTTCAG 
GCGTCACTAT TTTCTACTGT CAAGGAACAG GATACGCTAG CGCCAGTTTC TGACACACTG 
TACATCAATG GAATCAGGAT TCCCTATAGG CCACAAGTAA ATTCATTACA GTGGGCAAGA 
GGCACTGTAT ATTGTACTCC CGCTCAATTA AATGAATTAT TGGGTGGCGT GAATAGACCT 
GCATCCAAAT ACAAAGAGAG CATCATTGCT GAGGGAAGTT TCTCGGAGCG TTCTTCAGAC 
GATAATAATG CGAACCATCC GGAACACCAG TATACCAAGC CCACCCGTAA GGGCAGGAAC 
AGTAGTTACG GGGTACTAAG AAATGTTTCT CGAGCTGTAG AAACTAGATG GGACGCCGTT 
GAAGATAGAT TCAACGACTA TGCTACCGCC ATGGGAGAAA CAATGAACGA GGCTGTTGAG 
CAGACTGGAA AAGATGTAAT GAAAGGTGCC CTTGGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SRO7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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