RAP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RAP1
Entrez Gene ID	855505
Full Name	DNA-binding transcription factor RAP1
Synonyms	GRF1,TBA1,TUF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183054.1	NP_014183.1	DNA-binding transcription factor RAP1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RAP1	NP_014183.1
Kluyveromyces lactis	KLLA0D19294g	XP_453918.1
Eremothecium gossypii	AGOS_ABL180W	NP_982767.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that Rap1 contains an AD required for Rap1-dependent gene transcription. The Rap1(AS) variant will likely also be useful for studies of the functions of Rap1 in other biological pathways.
Title: Identification of a transcriptional activation domain in yeast repressor activator protein 1 (Rap1) using an altered DNA-binding specificity variant.

the yKu-distal portion of telomeres is bound by Rap1, which in turn reduces the potential for yKu(Yku80 and Yku70 )to mediate NHEJ. These results thus propose a solution to a long-standing conundrum, namely how to accommodate the apparently conflicting functions of Ku on telomeres.
Title: Ku Binding on Telomeres Occurs at Sites Distal from the Physical Chromosome Ends.

Rap1 (ScRap1) can either activate or repress transcription. This bimodal transcriptional activity has led to the widespread study of the mode of action of ScRap1. This review summarizes current knowledge about ScRap1, including its structure, mechanisms of transcription regulation, and biological functions, and the future directions in the field. Review.
Title: The multifunctional transcription factor Rap1: a regulator of yeast physiology.

Pif1, a 5'-3' helicase, not only stimulates the strand displacement activity of Pol delta but it also allows efficient replication through the Rap1 block, by removing bound Rap1 in front of the polymerase.
Title: Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase ?.

Rap1 contributes to telomere homeostasis by promoting chromosome breakage.
Title: Binding of Multiple Rap1 Proteins Stimulates Chromosome Breakage Induction during DNA Replication.

The following RAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04430	NM_001183054.1 Latest version!	Saccharomyces cerevisiae S288C DNA-binding transcription factor RAP1 (RAP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAP1 ( NM_001183054.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014183.1 Saccharomyces cerevisiae S288C DNA-binding transcription factor RAP1 (RAP1), partial mRNA.

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CloneID	OSi04430
Clone ID Related Accession (Same CDS sequence)	NM_001183054.1
Accession Version	NM_001183054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-binding transcription factor RAP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTAGTC CAGATGATTT TGAAACTGCA CCAGCAGAAT ATGTTGATGC ATTGGATCCG 
AGTATGGTCG TTGTTGACAG TGGTTCTGCT GCAGTCACAG CGCCATCGGA TTCAGCCGCT 
GAGGTAAAAG CTAATCAAAA CGAAGAAAAC ACTGGTGCTA CTGCTGCTGA AACTAGTGAG 
AAAGTGGATC AGACGGAGGT CGAAAAGAAA GATGATGATG ATACCACTGA AGTCGGTGTA 
ACAACTACCA CGCCTTCCAT TGCTGATACT GCTGCTACCG CTAACATTGC TTCGACATCA 
GGTGCATCTG TTACTGAACC TACTACTGAT GACACCGCTG CTGATGAGAA AAAGGAACAA 
GTAAGTGGTC CTCCTCTGTC AAATATGAAA TTCTATCTTA ATCGCGACGC GGATGCGCAT 
GACTCTTTAA ATGATATTGA TCAATTAGCG CGTCTGATTA GAGCAAACGG TGGTGAAGTG 
CTAGACTCCA AGCCAAGAGA ATCAAAAGAG AATGTTTTCA TCGTGTCACC CTATAATCAT 
ACCAATTTAC CAACGGTAAC TCCGACTTAT ATTAAGGCAT GTTGTCAAAG CAACTCGCTA 
CTGAACATGG AAAATTATTT AGTACCATAC GATAACTTTA GAGAAGTTGT CGATAGTAGG 
TTGCAGGAGG AATCGCATTC TAATGGTGTA GATAATAGCA ATAGCAATAG CGATAACAAG 
GATTCTATCA GGCCCAAAAC AGAAATAATC TCTACGAATA CTAATGGCGC TACGGAAGAC 
TCAACCAGCG AAAAAGTTAT GGTAGACGCT GAACAACAAG CTAGATTACA AGAGCAAGCT 
CAGCTCCTTC GTCAACATGT AAGCAGCACC GCATCAATCA CAAGCGGAGG GCACAATGAC 
TTGGTCCAGA TCGAACAACC TCAAAAAGAT ACCTCCAATA ATAATAATAG CAACGTCAAC 
GACGAAGACA ATGATCTTTT GACACAGGAC AACAACCCTC AAACAGCAGA TGAGGGGAAT 
GCATCTTTTC AAGCACAAAG GTCCATGATT TCGAGAGGCG CTTTGCCCTC CCACAATAAA 
GCTTCTTTTA CAGATGAGGA AGATGAGTTT ATTTTGGATG TTGTGAGAAA AAATCCAACC 
AGGCGTACAA CACATACTCT TTACGATGAA ATATCCCATT ATGTGCCTAA CCACACGGGT 
AATTCTATTA GGCACCGATT TAGAGTCTAT CTTTCCAAAA GACTAGAGTA CGTTTATGAG 
GTTGACAAGT TTGGTAAATT GGTTAGAGAC GACGATGGAA ATCTGATAAA GACTAAAGTT 
TTGCCACCAT CAATTAAAAG GAAATTTTCA GCAGATGAAG ATTACACTTT GGCAATCGCG 
GTCAAGAAGC AGTTTTATCG TGATTTGTTT CAAATCGACC CTGATACTGG AAGATCACTT 
ATCACAGATG AGGATACACC CACTGCTATA GCGAGGAGGA ATATGACAAT GGATCCAAAT 
CATGTGCCAG GGAGTGAACC CAACTTTGCA GCTTACAGAA CCCAAAGCCG TAGGGGACCG 
ATTGCACGAG AATTTTTCAA GCATTTTGCC GAAGAGCATG CAGCACATAC TGAGAATGCT 
TGGAGAGATA GGTTTAGGAA GTTTCTTCTC GCTTACGGCA TTGATGATTA TATTAGTTAT 
TATGAAGCTG AAAAAGCTCA GAATAGAGAG CCGGAACCGA TGAAGAACCT CACCAATAGA 
CCCAAGAGGC CTGGCGTTCC CACTCCTGGC AATTATAACT CTGCCGCCAA GAGGGCAAGA 
AATTATAGTT CTCAAAGAAA TGTTCAGCCT ACTGCCAATG CAGCGTCCGC TAATGCTGCT 
GCCGCTGCTG CTGCCGCTGC TTCCAACTCT TACGCTATAC CAGAAAACGA ATTACTGGAC 
GAAGATACCA TGAATTTCAT TTCCAGTTTA AAGAATGATC TATCCAATAT TTCAAATAGT 
TTGCCCTTTG AGTATCCACA CGAGATTGCG GAAGCTATAA GAAGCGATTT TTCGAATGAA 
GATATATATG ATAATATTGA TCCTGATACC ATAAGCTTTC CACCAAAAAT TGCAACAACA 
GATCTTTTCC TGCCATTGTT CTTTCATTTT GGCAGTACGA GACAATTTAT GGATAAACTT 
CATGAAGTTA TTTCAGGAGA TTATGAGCCA TCACAGGCTG AAAAACTGGT ACAGGATCTT 
TGCGATGAAA CTGGTATACG TAAAAATTTC AGTACGAGCA TATTGACGTG TTTATCCGGG 
GATCTGATGG TCTTTCCTCG CTATTTCTTG AACATGTTTA AGGACAATGT TAATCCTCCT 
CCCAACGTTC CTGGTATTTG GACACATGAT GACGACGAAT CGCTAAAAAG CAATGATCAA 
GAACAAATAA GGAAACTGGT TAAAAAGCAT GGAACTGGTA GAATGGAAAT GAGGAAAAGA 
TTTTTTGAGA AGGACCTGTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014183.1
CDS	1..2484
Translation	MSSPDDFETAPAEYVDALDPSMVVVDSGSAAVTAPSDSAAEVKANQNEENTGATAAETSEKVDQTEVEKKDDDDTTEVGVTTTTPSIADTAATANIASTSGASVTEPTTDDTAADEKKEQVSGPPLSNMKFYLNRDADAHDSLNDIDQLARLIRANGGEVLDSKPRESKENVFIVSPYNHTNLPTVTPTYIKACCQSNSLLNMENYLVPYDNFREVVDSRLQEESHSNGVDNSNSNSDNKDSIRPKTEIISTNTNGATEDSTSEKVMVDAEQQARLQEQAQLLRQHVSSTASITSGGHNDLVQIEQPQKDTSNNNNSNVNDEDNDLLTQDNNPQTADEGNASFQAQRSMISRGALPSHNKASFTDEEDEFILDVVRKNPTRRTTHTLYDEISHYVPNHTGNSIRHRFRVYLSKRLEYVYEVDKFGKLVRDDDGNLIKTKVLPPSIKRKFSADEDYTLAIAVKKQFYRDLFQIDPDTGRSLITDEDTPTAIARRNMTMDPNHVPGSEPNFAAYRTQSRRGPIAREFFKHFAEEHAAHTENAWRDRFRKFLLAYGIDDYISYYEAEKAQNREPEPMKNLTNRPKRPGVPTPGNYNSAAKRARNYSSQRNVQPTANAASANAAAAAAAAASNSYAIPENELLDEDTMNFISSLKNDLSNISNSLPFEYPHEIAEAIRSDFSNEDIYDNIDPDTISFPPKIATTDLFLPLFFHFGSTRQFMDKLHEVISGDYEPSQAEKLVQDLCDETGIRKNFSTSILTCLSGDLMVFPRYFLNMFKDNVNPPPNVPGIWTHDDDESLKSNDQEQIRKLVKKHGTGRMEMRKRFFEKDLL

Target ORF information:

RefSeq Version	NM_001183054.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-binding transcription factor RAP1 (RAP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183054.1

ORF Insert Sequence:

ATGTCTAGTC CAGATGATTT TGAAACTGCA CCAGCAGAAT ATGTTGATGC ATTGGATCCG 
AGTATGGTCG TTGTTGACAG TGGTTCTGCT GCAGTCACAG CGCCATCGGA TTCAGCCGCT 
GAGGTAAAAG CTAATCAAAA CGAAGAAAAC ACTGGTGCTA CTGCTGCTGA AACTAGTGAG 
AAAGTGGATC AGACGGAGGT CGAAAAGAAA GATGATGATG ATACCACTGA AGTCGGTGTA 
ACAACTACCA CGCCTTCCAT TGCTGATACT GCTGCTACCG CTAACATTGC TTCGACATCA 
GGTGCATCTG TTACTGAACC TACTACTGAT GACACCGCTG CTGATGAGAA AAAGGAACAA 
GTAAGTGGTC CTCCTCTGTC AAATATGAAA TTCTATCTTA ATCGCGACGC GGATGCGCAT 
GACTCTTTAA ATGATATTGA TCAATTAGCG CGTCTGATTA GAGCAAACGG TGGTGAAGTG 
CTAGACTCCA AGCCAAGAGA ATCAAAAGAG AATGTTTTCA TCGTGTCACC CTATAATCAT 
ACCAATTTAC CAACGGTAAC TCCGACTTAT ATTAAGGCAT GTTGTCAAAG CAACTCGCTA 
CTGAACATGG AAAATTATTT AGTACCATAC GATAACTTTA GAGAAGTTGT CGATAGTAGG 
TTGCAGGAGG AATCGCATTC TAATGGTGTA GATAATAGCA ATAGCAATAG CGATAACAAG 
GATTCTATCA GGCCCAAAAC AGAAATAATC TCTACGAATA CTAATGGCGC TACGGAAGAC 
TCAACCAGCG AAAAAGTTAT GGTAGACGCT GAACAACAAG CTAGATTACA AGAGCAAGCT 
CAGCTCCTTC GTCAACATGT AAGCAGCACC GCATCAATCA CAAGCGGAGG GCACAATGAC 
TTGGTCCAGA TCGAACAACC TCAAAAAGAT ACCTCCAATA ATAATAATAG CAACGTCAAC 
GACGAAGACA ATGATCTTTT GACACAGGAC AACAACCCTC AAACAGCAGA TGAGGGGAAT 
GCATCTTTTC AAGCACAAAG GTCCATGATT TCGAGAGGCG CTTTGCCCTC CCACAATAAA 
GCTTCTTTTA CAGATGAGGA AGATGAGTTT ATTTTGGATG TTGTGAGAAA AAATCCAACC 
AGGCGTACAA CACATACTCT TTACGATGAA ATATCCCATT ATGTGCCTAA CCACACGGGT 
AATTCTATTA GGCACCGATT TAGAGTCTAT CTTTCCAAAA GACTAGAGTA CGTTTATGAG 
GTTGACAAGT TTGGTAAATT GGTTAGAGAC GACGATGGAA ATCTGATAAA GACTAAAGTT 
TTGCCACCAT CAATTAAAAG GAAATTTTCA GCAGATGAAG ATTACACTTT GGCAATCGCG 
GTCAAGAAGC AGTTTTATCG TGATTTGTTT CAAATCGACC CTGATACTGG AAGATCACTT 
ATCACAGATG AGGATACACC CACTGCTATA GCGAGGAGGA ATATGACAAT GGATCCAAAT 
CATGTGCCAG GGAGTGAACC CAACTTTGCA GCTTACAGAA CCCAAAGCCG TAGGGGACCG 
ATTGCACGAG AATTTTTCAA GCATTTTGCC GAAGAGCATG CAGCACATAC TGAGAATGCT 
TGGAGAGATA GGTTTAGGAA GTTTCTTCTC GCTTACGGCA TTGATGATTA TATTAGTTAT 
TATGAAGCTG AAAAAGCTCA GAATAGAGAG CCGGAACCGA TGAAGAACCT CACCAATAGA 
CCCAAGAGGC CTGGCGTTCC CACTCCTGGC AATTATAACT CTGCCGCCAA GAGGGCAAGA 
AATTATAGTT CTCAAAGAAA TGTTCAGCCT ACTGCCAATG CAGCGTCCGC TAATGCTGCT 
GCCGCTGCTG CTGCCGCTGC TTCCAACTCT TACGCTATAC CAGAAAACGA ATTACTGGAC 
GAAGATACCA TGAATTTCAT TTCCAGTTTA AAGAATGATC TATCCAATAT TTCAAATAGT 
TTGCCCTTTG AGTATCCACA CGAGATTGCG GAAGCTATAA GAAGCGATTT TTCGAATGAA 
GATATATATG ATAATATTGA TCCTGATACC ATAAGCTTTC CACCAAAAAT TGCAACAACA 
GATCTTTTCC TGCCATTGTT CTTTCATTTT GGCAGTACGA GACAATTTAT GGATAAACTT 
CATGAAGTTA TTTCAGGAGA TTATGAGCCA TCACAGGCTG AAAAACTGGT ACAGGATCTT 
TGCGATGAAA CTGGTATACG TAAAAATTTC AGTACGAGCA TATTGACGTG TTTATCCGGG 
GATCTGATGG TCTTTCCTCG CTATTTCTTG AACATGTTTA AGGACAATGT TAATCCTCCT 
CCCAACGTTC CTGGTATTTG GACACATGAT GACGACGAAT CGCTAAAAAG CAATGATCAA 
GAACAAATAA GGAAACTGGT TAAAAAGCAT GGAACTGGTA GAATGGAAAT GAGGAAAAGA 
TTTTTTGAGA AGGACCTGTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RAP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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