TOF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TOF1
Entrez Gene ID	855448
Full Name	Tof1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183111.1	NP_014126.1	Tof1p

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	swi1	NP_595358.1
Magnaporthe oryzae (rice blast fungus)	MGG_03991	XP_003719862.1
Neurospora crassa	NCU09552	XP_958771.1
Saccharomyces cerevisiae (baker's yeast)	TOF1	NP_014126.1
Kluyveromyces lactis	KLLA0C02255g	XP_452297.1
Eremothecium gossypii	AGOS_AFR672C	NP_986220.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphorylation regulates the recruitment and retention of Tof1-Csm3 by the replisome and that this complex antagonizes the Rrm3 helicase, thereby promoting replication fork arrest.
Title: Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest.

although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin
Title: Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability.

Authors show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1.
Title: The role of CSM3, MRC1, and TOF1 in minisatellite stability and large loop DNA repair during meiosis in yeast.

Data show that that ISC1 controls cell morphology in combination with checkpoint regulators Swe1, Cdk1(CDC28), MRC1, TOF1, CSM3, RAD9 and Rad53.
Title: Cellular morphogenesis under stress is influenced by the sphingolipid pathway gene ISC1 and DNA integrity checkpoint genes in Saccharomyces cerevisiae.

Functional Tof1 protein, a replication fork-stabilizing protein, precludes expansions of various unstable repeats.
Title: Expansions, contractions, and fragility of the spinocerebellar ataxia type 10 pentanucleotide repeat in yeast.

The following TOF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04377	NM_001183111.1 Latest version!	Saccharomyces cerevisiae S288C Tof1p (TOF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOF1 ( NM_001183111.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014126.1 Saccharomyces cerevisiae S288C Tof1p (TOF1), partial mRNA.

Price & Availability↑

CloneID	OSi04377
Clone ID Related Accession (Same CDS sequence)	NM_001183111.1
Accession Version	NM_001183111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Tof1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGCTG ATTTGCAACA AGGCACTACA AATGCGGCTG ATTTCTCTCT GACGGTACTT 
AGAGCAAGAA TTGCTTTATT GGCAACTGCC ATCGGTGGGC CGGATTATAC TTCCCAGATA 
GACCCACCCC CTTATAAGCT TGGCGATGAC TGTTTGGCGT GCTTAAAAGA TCTGAAAAGA 
TGGTTTAAAC TAGTGGATGA CCAGCAGAAA AGATGGGATG TGGCAATGGC AGTAGCTGAA 
TATCGCATCT TGACGGATGA TCTGTTACCT ATTCTGATAG ATTGGGAAAA CAAATGTTCT 
CTTGCAGCAA AGCTTGCAAA GAACAACCCA GACCATGAAG AGTTTAGAAA CAAAGCTTAT 
TACGATAAAA TTGCGCTAAA TTGTCTACAA CTATTGGTCC TTATGACTTG GCCTTTAATT 
GTAACTGAAC AGTCTTCATC AAATCAAATT ACTCTCTATG GTGAGCTAAA GAAGCATCAA 
TTGGTATACA AGAAAACGAT TCTGTCTATG GAAAGTGGTA AAGTGTTAAG AGCTGCCATT 
AGATTGGCTC TGGATGTTAT CAAAATCGAC CGATTATCAA GAACTCCTAG GGACAATATG 
GTTCTCAAAT TGGTTTTGAA TTTTTTCAGA AATGTCATTG CTATAGAACC CGGTGAATTT 
ACTATCAATA CTAAGAAAAG CATGCCTAAG AAAGGTATCA CATCTATCGA TACTTTGCCA 
CCAAACGTCT CTATGGATGA CATCTCTTTA AATACGGTTA TTTCCTCATT CCATAAAAAC 
AAGGTATTCG GTTTCCTCTT AACGCTGACC AGCTCCTTAT CAAAAGAGTT TGACCAGGAC 
TTTATCAATA TCCCACTGTT GGAGATTATG TTCTACTTCA CTAAAGATGT TAACCAAGAA 
CTGCTATTCC CTCGACAGTT CGAAACCGGG ACACATTCAA AGGTGGTAAA TAAAAATGAG 
AGCTCCTCTG CTAATAACAT TGTTACAAGT GCAGGTTTTG AACTATCGAA GTTATTACAA 
AAGGAACACC AAATGAGAAA AAACGTTATC AAGCATACAT CTGCAAGACA CTCCAGATTT 
GGTGGTCTTT TATCTATACA AACACCAGAT AAAACTAGGT TAACTGTTTC TGGTAGCCAA 
GCTCTCGTAG ATGAAAAAAT AGCGCTCCAA AAACTAGATG ACAGCAAAAA ATGGAATAAA 
AGAATAATTA AAAAGCATCA ATCTGTCGCA GCTGAGGGCT TACCGAATAG TCTCTTGAAT 
TCACAAACCG GTAAAGCTAT TTTTTTCACC GAATCAAATG GTAAACATTT CAAAGAATTC 
ATCAACAACT TTATAGATTC CGGCTTTAAC ATCTTGTTGC ATAGTGTGAC GAACTACTTC 
ACAACTGAGC AGGATAGGAT GGTAACTTTG GAGCAAGTCG AATATTTACT TTTCTTTGCA 
TGGTTTGTTA AATACCAGTT GTTGAGGTCC AAAATCGACA ATTCCGCAGA TATAAAGCAA 
GTTTCAGAAG CCTTGAAAGA GGTGACTTTC ATTCTAGTGT CTTCTCTTTT AAGAAGCGCA 
TATGACCTGA AAAATTGGAC AGTGACACAT GCTGGTATGA TTGCATTTAA TGAACTGTTG 
AACCTCGTTT CCCGTACAAA GGCCGCTCAA GAAGAGGATT CGACCGATAT AGAATTCATT 
GTAAGTAGGC TTTTCAGTGA CGAGAGAATA CAATTACTGT CAAATCTGCC GAAGATTGGT 
TCCAAATACT CCCTCCAATT CATGAAAAGT TGCATTGAAC TAACACACTC GGTATTGAAA 
GTATTAGAGC AATACTCTGA CGATAAAACA TTAGTTATTG AAGGCAAATC AAGACGGCAA 
AAAAAGTTTA ATATTTCAGA AGGTGATATT ACTAAATTGA TAGAAGAAGA AAATGTAGAT 
AGAGATGAAG CGCTTGATAT TCTAACCTCT TCTTTAAGAA GTATAGAAGT CAATTTTCAA 
AAGGTGCAAG CCAACTATAT GACAGAACCG GTAATAGAAA CGTACATTAA TTTCCTTGAA 
AGATTTCGTG AGCTTGAGGA CGATTCCATT AAAAAAGTAT TTTCCTTTTT CCATAGAGTA 
TTTGTTCAGG CAAAAGAACA GGCATTGTTA TTTAGATTTG ATTTGATAAT ATTATTAAGA 
GAAATGCTTT CACCTGACGG ATTAGATAGA ATGTCTCGTT CAAGGAAATA TGTGAGTCAA 
TTTTCAGACT ACTTCCTCGC CAGGTTGAAG AAAAGGTTAA AGAAATCACC TGCTTGGTTT 
GTTGGATTAC TTTTCCCGCC ATTACACAAT AGTGAAGTAG GATTTTACCA AAGATATGGT 
GAATATAATG TACTTAATAA TGAATCAATG TATGCCGCTC CTGCATCACA GTTCAAACCA 
ATTCCCGATG AAGAAGCCTT ACCACCTTCG ATTTTGTTGG ATATGAAATA TGGTGTTTTA 
GTTTCCACAT TACTTGATGA TGGAAAAACT GAACTATTGG ACCAACTGCT CAAGCATATA 
ACCCACACAC TAGATATTTT TAAGTCGTGG CTAACCGTAA ATGTTAATGC TGGGAAAGAA 
ACAGTTAATC CACCCAATGA ATATTTCACA TTGACAGGTG TACTCAACAA CGACCCTATT 
TTTAAAGATA AGGACTACAG GGCTTTATTG TTGCTGATAG GATACTCCAT TCCACGTAAA 
ATCAATGAAC CTTGTTTTTT ACCGGGGACT GTTGAAGTTT CTGACCTGAC TGTTTCTTGT 
GAACTAGTGA AAAAATACTT ATCAACACCA TTTGAAACTC CAAACGGACT GCCTTCTTCT 
TCCTATCTTC TACGAGTTCG TTCAGAAAAG GATAGTTTTT CTCACAATGA ACAAGATGGA 
TGGGAAGGAG ACGATGATTA TGATTACAAC GATCCCTACA TTGTTCCAGA TGATCAAATT 
TTATCGAAAA GCGACGCAGC ATACTTCAAG GATTTAGATA ACAATGCATC AGATAAACTT 
AAAGGTACCA AATTCAGTAA AGGGATTGCG AGATCCAAAA AAAAAGATAA GAGGAAGCGT 
AGGAAAGGAG AAGCCAAGAC TAATTTGCCA ATGTTTGGCG ACCAGGATGA TGAACGACCT 
CAGACCGTTA GAGAACGCCA TGGCGTATTC AGTAAAGAGT TTATTAGTGA TTCAGAAGAT 
GACGAGGATT TGATGAACCC TATATTTTTT GAAAACGAAA CATATATGAG ATGGTTACTA 
GATAAAAACA ATGGTCAGTT GACCGAGGAT AGATATATCC AGTTTGCCAA ATTTGCCGCG 
GAAAGGATGA ATAATGGGGG TGTTGTGACA GGAGATTATA CGAGCTTGTT TGGTGGATCC 
ATACCAAGTA TTGAAAGCAT AAGAGCTACA GAAAGTAGTT CCTTTGCGCC AGATAAAAGC 
CTCATTTCTT TAGCAAGCCA CGTGGCATCT GAAATGTCTA TTTTCGATGT AAATAATAAC 
AATAATAATC AACTCTCTGA TGACGATGTC AACTCGGAAT CAAGAAATAG TTTGGGCTCA 
TCACAGCCAT CAAATTCGCA AAATATGTTC CAATCTGAGG TGTATAGCAG AAAAGAATCT 
ACAAAGCGTT CTTTGGAAGC GAGTGCAGCC GATGAAAGCG ATGAAGATGA AGAGGCCATC 
CGCCTTTTTG GCAAAAAGTC TAGAGTTGTT TTGAGCCAAG GTGATAGTGA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014126.1
CDS	1..3717
Translation	MSADLQQGTTNAADFSLTVLRARIALLATAIGGPDYTSQIDPPPYKLGDDCLACLKDLKRWFKLVDDQQKRWDVAMAVAEYRILTDDLLPILIDWENKCSLAAKLAKNNPDHEEFRNKAYYDKIALNCLQLLVLMTWPLIVTEQSSSNQITLYGELKKHQLVYKKTILSMESGKVLRAAIRLALDVIKIDRLSRTPRDNMVLKLVLNFFRNVIAIEPGEFTINTKKSMPKKGITSIDTLPPNVSMDDISLNTVISSFHKNKVFGFLLTLTSSLSKEFDQDFINIPLLEIMFYFTKDVNQELLFPRQFETGTHSKVVNKNESSSANNIVTSAGFELSKLLQKEHQMRKNVIKHTSARHSRFGGLLSIQTPDKTRLTVSGSQALVDEKIALQKLDDSKKWNKRIIKKHQSVAAEGLPNSLLNSQTGKAIFFTESNGKHFKEFINNFIDSGFNILLHSVTNYFTTEQDRMVTLEQVEYLLFFAWFVKYQLLRSKIDNSADIKQVSEALKEVTFILVSSLLRSAYDLKNWTVTHAGMIAFNELLNLVSRTKAAQEEDSTDIEFIVSRLFSDERIQLLSNLPKIGSKYSLQFMKSCIELTHSVLKVLEQYSDDKTLVIEGKSRRQKKFNISEGDITKLIEEENVDRDEALDILTSSLRSIEVNFQKVQANYMTEPVIETYINFLERFRELEDDSIKKVFSFFHRVFVQAKEQALLFRFDLIILLREMLSPDGLDRMSRSRKYVSQFSDYFLARLKKRLKKSPAWFVGLLFPPLHNSEVGFYQRYGEYNVLNNESMYAAPASQFKPIPDEEALPPSILLDMKYGVLVSTLLDDGKTELLDQLLKHITHTLDIFKSWLTVNVNAGKETVNPPNEYFTLTGVLNNDPIFKDKDYRALLLLIGYSIPRKINEPCFLPGTVEVSDLTVSCELVKKYLSTPFETPNGLPSSSYLLRVRSEKDSFSHNEQDGWEGDDDYDYNDPYIVPDDQILSKSDAAYFKDLDNNASDKLKGTKFSKGIARSKKKDKRKRRKGEAKTNLPMFGDQDDERPQTVRERHGVFSKEFISDSEDDEDLMNPIFFENETYMRWLLDKNNGQLTEDRYIQFAKFAAERMNNGGVVTGDYTSLFGGSIPSIESIRATESSSFAPDKSLISLASHVASEMSIFDVNNNNNNQLSDDDVNSESRNSLGSSQPSNSQNMFQSEVYSRKESTKRSLEASAADESDEDEEAIRLFGKKSRVVLSQGDSDD

Target ORF information:

RefSeq Version	NM_001183111.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Tof1p (TOF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183111.1

ORF Insert Sequence:

ATGTCTGCTG ATTTGCAACA AGGCACTACA AATGCGGCTG ATTTCTCTCT GACGGTACTT 
AGAGCAAGAA TTGCTTTATT GGCAACTGCC ATCGGTGGGC CGGATTATAC TTCCCAGATA 
GACCCACCCC CTTATAAGCT TGGCGATGAC TGTTTGGCGT GCTTAAAAGA TCTGAAAAGA 
TGGTTTAAAC TAGTGGATGA CCAGCAGAAA AGATGGGATG TGGCAATGGC AGTAGCTGAA 
TATCGCATCT TGACGGATGA TCTGTTACCT ATTCTGATAG ATTGGGAAAA CAAATGTTCT 
CTTGCAGCAA AGCTTGCAAA GAACAACCCA GACCATGAAG AGTTTAGAAA CAAAGCTTAT 
TACGATAAAA TTGCGCTAAA TTGTCTACAA CTATTGGTCC TTATGACTTG GCCTTTAATT 
GTAACTGAAC AGTCTTCATC AAATCAAATT ACTCTCTATG GTGAGCTAAA GAAGCATCAA 
TTGGTATACA AGAAAACGAT TCTGTCTATG GAAAGTGGTA AAGTGTTAAG AGCTGCCATT 
AGATTGGCTC TGGATGTTAT CAAAATCGAC CGATTATCAA GAACTCCTAG GGACAATATG 
GTTCTCAAAT TGGTTTTGAA TTTTTTCAGA AATGTCATTG CTATAGAACC CGGTGAATTT 
ACTATCAATA CTAAGAAAAG CATGCCTAAG AAAGGTATCA CATCTATCGA TACTTTGCCA 
CCAAACGTCT CTATGGATGA CATCTCTTTA AATACGGTTA TTTCCTCATT CCATAAAAAC 
AAGGTATTCG GTTTCCTCTT AACGCTGACC AGCTCCTTAT CAAAAGAGTT TGACCAGGAC 
TTTATCAATA TCCCACTGTT GGAGATTATG TTCTACTTCA CTAAAGATGT TAACCAAGAA 
CTGCTATTCC CTCGACAGTT CGAAACCGGG ACACATTCAA AGGTGGTAAA TAAAAATGAG 
AGCTCCTCTG CTAATAACAT TGTTACAAGT GCAGGTTTTG AACTATCGAA GTTATTACAA 
AAGGAACACC AAATGAGAAA AAACGTTATC AAGCATACAT CTGCAAGACA CTCCAGATTT 
GGTGGTCTTT TATCTATACA AACACCAGAT AAAACTAGGT TAACTGTTTC TGGTAGCCAA 
GCTCTCGTAG ATGAAAAAAT AGCGCTCCAA AAACTAGATG ACAGCAAAAA ATGGAATAAA 
AGAATAATTA AAAAGCATCA ATCTGTCGCA GCTGAGGGCT TACCGAATAG TCTCTTGAAT 
TCACAAACCG GTAAAGCTAT TTTTTTCACC GAATCAAATG GTAAACATTT CAAAGAATTC 
ATCAACAACT TTATAGATTC CGGCTTTAAC ATCTTGTTGC ATAGTGTGAC GAACTACTTC 
ACAACTGAGC AGGATAGGAT GGTAACTTTG GAGCAAGTCG AATATTTACT TTTCTTTGCA 
TGGTTTGTTA AATACCAGTT GTTGAGGTCC AAAATCGACA ATTCCGCAGA TATAAAGCAA 
GTTTCAGAAG CCTTGAAAGA GGTGACTTTC ATTCTAGTGT CTTCTCTTTT AAGAAGCGCA 
TATGACCTGA AAAATTGGAC AGTGACACAT GCTGGTATGA TTGCATTTAA TGAACTGTTG 
AACCTCGTTT CCCGTACAAA GGCCGCTCAA GAAGAGGATT CGACCGATAT AGAATTCATT 
GTAAGTAGGC TTTTCAGTGA CGAGAGAATA CAATTACTGT CAAATCTGCC GAAGATTGGT 
TCCAAATACT CCCTCCAATT CATGAAAAGT TGCATTGAAC TAACACACTC GGTATTGAAA 
GTATTAGAGC AATACTCTGA CGATAAAACA TTAGTTATTG AAGGCAAATC AAGACGGCAA 
AAAAAGTTTA ATATTTCAGA AGGTGATATT ACTAAATTGA TAGAAGAAGA AAATGTAGAT 
AGAGATGAAG CGCTTGATAT TCTAACCTCT TCTTTAAGAA GTATAGAAGT CAATTTTCAA 
AAGGTGCAAG CCAACTATAT GACAGAACCG GTAATAGAAA CGTACATTAA TTTCCTTGAA 
AGATTTCGTG AGCTTGAGGA CGATTCCATT AAAAAAGTAT TTTCCTTTTT CCATAGAGTA 
TTTGTTCAGG CAAAAGAACA GGCATTGTTA TTTAGATTTG ATTTGATAAT ATTATTAAGA 
GAAATGCTTT CACCTGACGG ATTAGATAGA ATGTCTCGTT CAAGGAAATA TGTGAGTCAA 
TTTTCAGACT ACTTCCTCGC CAGGTTGAAG AAAAGGTTAA AGAAATCACC TGCTTGGTTT 
GTTGGATTAC TTTTCCCGCC ATTACACAAT AGTGAAGTAG GATTTTACCA AAGATATGGT 
GAATATAATG TACTTAATAA TGAATCAATG TATGCCGCTC CTGCATCACA GTTCAAACCA 
ATTCCCGATG AAGAAGCCTT ACCACCTTCG ATTTTGTTGG ATATGAAATA TGGTGTTTTA 
GTTTCCACAT TACTTGATGA TGGAAAAACT GAACTATTGG ACCAACTGCT CAAGCATATA 
ACCCACACAC TAGATATTTT TAAGTCGTGG CTAACCGTAA ATGTTAATGC TGGGAAAGAA 
ACAGTTAATC CACCCAATGA ATATTTCACA TTGACAGGTG TACTCAACAA CGACCCTATT 
TTTAAAGATA AGGACTACAG GGCTTTATTG TTGCTGATAG GATACTCCAT TCCACGTAAA 
ATCAATGAAC CTTGTTTTTT ACCGGGGACT GTTGAAGTTT CTGACCTGAC TGTTTCTTGT 
GAACTAGTGA AAAAATACTT ATCAACACCA TTTGAAACTC CAAACGGACT GCCTTCTTCT 
TCCTATCTTC TACGAGTTCG TTCAGAAAAG GATAGTTTTT CTCACAATGA ACAAGATGGA 
TGGGAAGGAG ACGATGATTA TGATTACAAC GATCCCTACA TTGTTCCAGA TGATCAAATT 
TTATCGAAAA GCGACGCAGC ATACTTCAAG GATTTAGATA ACAATGCATC AGATAAACTT 
AAAGGTACCA AATTCAGTAA AGGGATTGCG AGATCCAAAA AAAAAGATAA GAGGAAGCGT 
AGGAAAGGAG AAGCCAAGAC TAATTTGCCA ATGTTTGGCG ACCAGGATGA TGAACGACCT 
CAGACCGTTA GAGAACGCCA TGGCGTATTC AGTAAAGAGT TTATTAGTGA TTCAGAAGAT 
GACGAGGATT TGATGAACCC TATATTTTTT GAAAACGAAA CATATATGAG ATGGTTACTA 
GATAAAAACA ATGGTCAGTT GACCGAGGAT AGATATATCC AGTTTGCCAA ATTTGCCGCG 
GAAAGGATGA ATAATGGGGG TGTTGTGACA GGAGATTATA CGAGCTTGTT TGGTGGATCC 
ATACCAAGTA TTGAAAGCAT AAGAGCTACA GAAAGTAGTT CCTTTGCGCC AGATAAAAGC 
CTCATTTCTT TAGCAAGCCA CGTGGCATCT GAAATGTCTA TTTTCGATGT AAATAATAAC 
AATAATAATC AACTCTCTGA TGACGATGTC AACTCGGAAT CAAGAAATAG TTTGGGCTCA 
TCACAGCCAT CAAATTCGCA AAATATGTTC CAATCTGAGG TGTATAGCAG AAAAGAATCT 
ACAAAGCGTT CTTTGGAAGC GAGTGCAGCC GATGAAAGCG ATGAAGATGA AGAGGCCATC 
CGCCTTTTTG GCAAAAAGTC TAGAGTTGTT TTGAGCCAAG GTGATAGTGA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TOF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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