MID1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MID1
Entrez Gene ID	855425
Full Name	Mid1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183129.1	NP_014108.1	Mid1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E13179g	XP_454544.1
Eremothecium gossypii	AGOS_AEL197C	NP_984664.2
Saccharomyces cerevisiae (baker's yeast)	MID1	NP_014108.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Therefore, H(+) extrusion by Pma1 may be important for Ca(2+) influx through Cch1/Mid1. These results suggest that Pma1 interacts physically with Cch1/Mid1 Ca(2+) channels to enhance their activity via its H(+) -pumping activity
Title: Coupling of a voltage-gated Ca

Cch1 and Mid1 mediate the ethanol-induced Ca2+ flux.
Title: Ethanol induces calcium influx via the Cch1-Mid1 transporter in Saccharomyces cerevisiae.

Growth of mutants in the Cch1pMid1p channel is markedly hypersensitive to low temperature and to high iron concentration in the medium.
Title: The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity.

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04359	NM_001183129.1 Latest version!	Saccharomyces cerevisiae S288C Mid1p (MID1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID1 ( NM_001183129.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014108.1 Saccharomyces cerevisiae S288C Mid1p (MID1), partial mRNA.

Price & Availability↑

CloneID	OSi04359
Clone ID Related Accession (Same CDS sequence)	NM_001183129.1
Accession Version	NM_001183129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mid1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATAGTGT GGCAAGCACT ATTCGTGGTT TACTGCCTAT TTACCACTTC TATTCACGGT 
TTATTCCAAG ACTTCAATCC TTTCGCAAAT AAGAATATTT CCTTAAAGTT TCCCAGCCTA 
AATAGATGGG AGAAAAACGT TATGGCTACT GGTCAACAAA CAATCATCAA TTCGGATAGC 
ATTTATGAAT GGACCCCGAT CTTATCTAAC ATAACAGCGG GCAAAAAAGA CAGTTTTGTA 
TTTACCATTG ACGCAGAAGC CTCCGGTTAT GGGTTTGCTC CCACGTATGA AGTGTTAATG 
TTTATTAGCG GCAATATATG CCAAATGCCC ATGAATAGAT CAGATGTGGA TTTAACAATA 
TACTACTCTT TTAATGAAAC GGTTTTAGAG AACCCAAATA TAGGTCAAAG TGCAGTTTTT 
CAGGATGGCT ACATCCAAGC ATTAGCCATC AGTCCAGTGC AGTCTTCTAG CTCGAACGCT 
ACCTCCACGT ATTCAAATCT TTATGTGGTC GCTGAGCTAG TGAATTCCAC TACGGAGCAA 
CCTCTGTCCA GTTCCGACGC CTCTGAAAAT TGGGAATATA GACTGAGTAT CTCTGAGAAC 
GATTTGGTTT TTCAGTGGGA TGTAAGGCCT TGGGTAGAGG TCCTGGACAC TGATATGAAT 
TCTGCATTAC TATCAACGGG GAATGTCACC GCAGACGCCA AAGTTTATCA CAATTATTCA 
ATATATGATC CATCCTTGTA CGATCTGTAT GTTTACTCGT ACGAAGATTC TGTTCAACTA 
AACCAAAACT ATAATTTATC TCTTTGTGCT GTGAAAAATG GACCATATTT AGTGTCTTCC 
CAAAATACAT CAAATGCAAC AGTTACGTCA AATAGTACAA ATCCTTTAGA GCGTACTGAC 
TTGGCGATTC AAAAAAAAAT TACAGAATAT GGTGGTAGCG TGACAGAAAT GTTTTACGTG 
ACAGGACTGA ATGCCTCAAC AACTTATGTA GCGTACCTGA CAAAGAAAAT CAGTAATGGT 
GATGGGTTGT CAAGTGTGGG CGGCATTTTA TTTTCACATG TTTATTTTAC CACAAGAAGT 
ACCGATGTCT GCTCTTTGAT CTTTGGTTTG GACTTCTGCA GTGACGTCGC TTACTCTGTT 
CCTACATCCT CATTTTCTGT GGGTAATAAA ACTCTCATGG CGCAGACATA TGACCACATT 
GCAGAGGCAC TATATGCAAA TTTTAGCAAA GCTCTACAGT TAATATCCTG TGACGCAGAC 
AAAGACGCAA GATATTCTCC GGTCATGACT TGTGACGATT GTGCCGAAGC TTATCGTGAT 
TGGGTATGTG CAGTATCAAT TCCGAGATGC ACCACGACGT CTTCCCAATA CTACATCCAC 
AGAGATAAAA GTCACAACCG TAATGATTAT TTGAATAAAT TTATTAAACC TTTGGATGAC 
TATTACGAAA TTCTACCTTG TATTGACATG TGCTATACAT TAGTACGAAA TTGCCCAAGC 
GATTTTCAAT TTTCCTGTCC GAATGATCTT ACTACGGAAG ATCTTCTTTA TCAAAGTTAC 
AATTTCTATA TGGACACTGA CTACTCAACC TGTAATTATA TAGGTAACTC ATCCTTGATG 
GTAATTCATC CATTGGACGA TACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014108.1
CDS	1..1647
Translation	MIVWQALFVVYCLFTTSIHGLFQDFNPFANKNISLKFPSLNRWEKNVMATGQQTIINSDSIYEWTPILSNITAGKKDSFVFTIDAEASGYGFAPTYEVLMFISGNICQMPMNRSDVDLTIYYSFNETVLENPNIGQSAVFQDGYIQALAISPVQSSSSNATSTYSNLYVVAELVNSTTEQPLSSSDASENWEYRLSISENDLVFQWDVRPWVEVLDTDMNSALLSTGNVTADAKVYHNYSIYDPSLYDLYVYSYEDSVQLNQNYNLSLCAVKNGPYLVSSQNTSNATVTSNSTNPLERTDLAIQKKITEYGGSVTEMFYVTGLNASTTYVAYLTKKISNGDGLSSVGGILFSHVYFTTRSTDVCSLIFGLDFCSDVAYSVPTSSFSVGNKTLMAQTYDHIAEALYANFSKALQLISCDADKDARYSPVMTCDDCAEAYRDWVCAVSIPRCTTTSSQYYIHRDKSHNRNDYLNKFIKPLDDYYEILPCIDMCYTLVRNCPSDFQFSCPNDLTTEDLLYQSYNFYMDTDYSTCNYIGNSSLMVIHPLDDT

Target ORF information:

RefSeq Version	NM_001183129.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mid1p (MID1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183129.1

ORF Insert Sequence:

ATGATAGTGT GGCAAGCACT ATTCGTGGTT TACTGCCTAT TTACCACTTC TATTCACGGT 
TTATTCCAAG ACTTCAATCC TTTCGCAAAT AAGAATATTT CCTTAAAGTT TCCCAGCCTA 
AATAGATGGG AGAAAAACGT TATGGCTACT GGTCAACAAA CAATCATCAA TTCGGATAGC 
ATTTATGAAT GGACCCCGAT CTTATCTAAC ATAACAGCGG GCAAAAAAGA CAGTTTTGTA 
TTTACCATTG ACGCAGAAGC CTCCGGTTAT GGGTTTGCTC CCACGTATGA AGTGTTAATG 
TTTATTAGCG GCAATATATG CCAAATGCCC ATGAATAGAT CAGATGTGGA TTTAACAATA 
TACTACTCTT TTAATGAAAC GGTTTTAGAG AACCCAAATA TAGGTCAAAG TGCAGTTTTT 
CAGGATGGCT ACATCCAAGC ATTAGCCATC AGTCCAGTGC AGTCTTCTAG CTCGAACGCT 
ACCTCCACGT ATTCAAATCT TTATGTGGTC GCTGAGCTAG TGAATTCCAC TACGGAGCAA 
CCTCTGTCCA GTTCCGACGC CTCTGAAAAT TGGGAATATA GACTGAGTAT CTCTGAGAAC 
GATTTGGTTT TTCAGTGGGA TGTAAGGCCT TGGGTAGAGG TCCTGGACAC TGATATGAAT 
TCTGCATTAC TATCAACGGG GAATGTCACC GCAGACGCCA AAGTTTATCA CAATTATTCA 
ATATATGATC CATCCTTGTA CGATCTGTAT GTTTACTCGT ACGAAGATTC TGTTCAACTA 
AACCAAAACT ATAATTTATC TCTTTGTGCT GTGAAAAATG GACCATATTT AGTGTCTTCC 
CAAAATACAT CAAATGCAAC AGTTACGTCA AATAGTACAA ATCCTTTAGA GCGTACTGAC 
TTGGCGATTC AAAAAAAAAT TACAGAATAT GGTGGTAGCG TGACAGAAAT GTTTTACGTG 
ACAGGACTGA ATGCCTCAAC AACTTATGTA GCGTACCTGA CAAAGAAAAT CAGTAATGGT 
GATGGGTTGT CAAGTGTGGG CGGCATTTTA TTTTCACATG TTTATTTTAC CACAAGAAGT 
ACCGATGTCT GCTCTTTGAT CTTTGGTTTG GACTTCTGCA GTGACGTCGC TTACTCTGTT 
CCTACATCCT CATTTTCTGT GGGTAATAAA ACTCTCATGG CGCAGACATA TGACCACATT 
GCAGAGGCAC TATATGCAAA TTTTAGCAAA GCTCTACAGT TAATATCCTG TGACGCAGAC 
AAAGACGCAA GATATTCTCC GGTCATGACT TGTGACGATT GTGCCGAAGC TTATCGTGAT 
TGGGTATGTG CAGTATCAAT TCCGAGATGC ACCACGACGT CTTCCCAATA CTACATCCAC 
AGAGATAAAA GTCACAACCG TAATGATTAT TTGAATAAAT TTATTAAACC TTTGGATGAC 
TATTACGAAA TTCTACCTTG TATTGACATG TGCTATACAT TAGTACGAAA TTGCCCAAGC 
GATTTTCAAT TTTCCTGTCC GAATGATCTT ACTACGGAAG ATCTTCTTTA TCAAAGTTAC 
AATTTCTATA TGGACACTGA CTACTCAACC TGTAATTATA TAGGTAACTC ATCCTTGATG 
GTAATTCATC CATTGGACGA TACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MID1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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