ORF » Species Summary » Saccharomyces cerevisiae S288C » PUS4 cDNA ORF clone

PUS4 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following PUS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04358 NM_001183130.1
Latest version!		 Saccharomyces cerevisiae S288C pseudouridine synthase PUS4 (PUS4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PUS4 ( NM_001183130.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014107.1 Saccharomyces cerevisiae S288C pseudouridine synthase PUS4 (PUS4), partial mRNA.
    Price & Availability↑
    CloneID OSi04358
    Clone ID Related Accession (Same CDS sequence) NM_001183130.1
    Accession Version NM_001183130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product pseudouridine synthase PUS4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGGAA TATTTGCTAT TGAGAAGCCT AGCGGTATTA CATCCAATCA GTTTATGCTA 
    AAGTTGCAAC ATGCTTTAAC TAAAAGTCAA GTATTCTCGA AGGAAATTCA AAGGGCAACA 
    GCGGAAAGAA AGCAGCAATA TGAAAAACAG ACAGGTAAGA AGGCCAGCAA GAGGAAACTT 
    CGTAAAGTCT CAAAGGTAAA GATGGGACAC GGAGGCACTT TGGATCCGTT GGCCTCTGGT 
    GTCCTGGTAA TTGGGATTGG AGCGGGCACC AAAAAACTTG CAAATTATCT TTCAGGCACC 
    GTTAAAGTCT ATGAAAGTGA AGCCCTGTTT GGTGTTTCCA CTACTTCAGG AGATGTAGAA 
    GGTGAGATTT TGTCCCAGAA CTCCGTTAAA CATTTAAATT TCGATGATTT AAAGACTGTG 
    GAGGAAAAAT TTGTTGGACA ATTAAAGCAG ACGCCGCCAA TATACGCAGC TTTGAAGATG 
    GATGGCAAAC CCCTGCATGA ATATGCTCGA GAAGGAAAGC CTTTACCTAG AGCCATTGAG 
    CCCAGACAAG TTACTATTTA TGACTTGAAG GTGTTTTCAG ACTCTCTTAA ACGTGATCAT 
    GACTATCCCT TATTGAGGCC CACTACAGAA GAAGCTGTTG ATACCGTGAA AAATTTGAAT 
    GCAAACATGC TAAACGATGT TTTATACTTT TCAAAAGAAT ATACAGAGAA GCACGGCCTT 
    GATTCTGAGG TGGCAAAGGT AGAAGAACCA TTTCCGCTGA GTGAACAAGA AGAGCAAGAA 
    ATCCAAAAAG AAGGAGATTC TTATAGGGCT CCAAAATTGC ACTTCAAAGC TAATGTGTCT 
    TCTGGAACAT ACATAAGGTC CTTAGTGAGC GACATCGGTA AATCTATGAG GAGCTCATGT 
    TATATGGTGA AATTGATACG TTTGCAACAG CAGGACTGGT CTCTTGAAAA AAATAATGTG 
    TTCCAATTAA CGGATTTTAC TGAAAGAGAC GAGAAGGTGT GGAGTAAGGT ACTGGAGAAG 
    GTTCTAGACG AGGGAGCAAC TGTTGATGTT ATAGAAGAGC TAAAGAAAGC AGAAAAGGAA 
    ATACCAGCGG ACGTGAAGGA ATGTATAGTT TCGAGTGATC AACCTGGTGA TGAGGCTACG 
    GCTGAAACAA TTGAAACTGC TAACGCCGAA GAACATTCTA ATACACTAAA AAGAAAAATC 
    GAACAGGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014107.1
    CDS1..1212
    Translation

    Target ORF information:

    RefSeq Version NM_001183130.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C pseudouridine synthase PUS4 (PUS4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001183130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGGAA TATTTGCTAT TGAGAAGCCT AGCGGTATTA CATCCAATCA GTTTATGCTA 
    AAGTTGCAAC ATGCTTTAAC TAAAAGTCAA GTATTCTCGA AGGAAATTCA AAGGGCAACA 
    GCGGAAAGAA AGCAGCAATA TGAAAAACAG ACAGGTAAGA AGGCCAGCAA GAGGAAACTT 
    CGTAAAGTCT CAAAGGTAAA GATGGGACAC GGAGGCACTT TGGATCCGTT GGCCTCTGGT 
    GTCCTGGTAA TTGGGATTGG AGCGGGCACC AAAAAACTTG CAAATTATCT TTCAGGCACC 
    GTTAAAGTCT ATGAAAGTGA AGCCCTGTTT GGTGTTTCCA CTACTTCAGG AGATGTAGAA 
    GGTGAGATTT TGTCCCAGAA CTCCGTTAAA CATTTAAATT TCGATGATTT AAAGACTGTG 
    GAGGAAAAAT TTGTTGGACA ATTAAAGCAG ACGCCGCCAA TATACGCAGC TTTGAAGATG 
    GATGGCAAAC CCCTGCATGA ATATGCTCGA GAAGGAAAGC CTTTACCTAG AGCCATTGAG 
    CCCAGACAAG TTACTATTTA TGACTTGAAG GTGTTTTCAG ACTCTCTTAA ACGTGATCAT 
    GACTATCCCT TATTGAGGCC CACTACAGAA GAAGCTGTTG ATACCGTGAA AAATTTGAAT 
    GCAAACATGC TAAACGATGT TTTATACTTT TCAAAAGAAT ATACAGAGAA GCACGGCCTT 
    GATTCTGAGG TGGCAAAGGT AGAAGAACCA TTTCCGCTGA GTGAACAAGA AGAGCAAGAA 
    ATCCAAAAAG AAGGAGATTC TTATAGGGCT CCAAAATTGC ACTTCAAAGC TAATGTGTCT 
    TCTGGAACAT ACATAAGGTC CTTAGTGAGC GACATCGGTA AATCTATGAG GAGCTCATGT 
    TATATGGTGA AATTGATACG TTTGCAACAG CAGGACTGGT CTCTTGAAAA AAATAATGTG 
    TTCCAATTAA CGGATTTTAC TGAAAGAGAC GAGAAGGTGT GGAGTAAGGT ACTGGAGAAG 
    GTTCTAGACG AGGGAGCAAC TGTTGATGTT ATAGAAGAGC TAAAGAAAGC AGAAAAGGAA 
    ATACCAGCGG ACGTGAAGGA ATGTATAGTT TCGAGTGATC AACCTGGTGA TGAGGCTACG 
    GCTGAAACAA TTGAAACTGC TAACGCCGAA GAACATTCTA ATACACTAAA AAGAAAAATC 
    GAACAGGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
    Nature387(6632 Suppl)93-8(1997 May)
    Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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