YTA12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YTA12
Entrez Gene ID	855114
Full Name	m-AAA protease subunit YTA12
Synonyms	RCA1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182589.1	NP_013807.1	m-AAA protease subunit YTA12

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC532875	XP_002694913.2
Saccharomyces cerevisiae (baker's yeast)	YTA12	NP_013807.1
Magnaporthe oryzae (rice blast fungus)	MGG_07441	XP_003711337.1
Mus musculus (house mouse)	Afg3l1	NP_473411.2
Kluyveromyces lactis	KLLA0F13706g	XP_455697.1
Schizosaccharomyces pombe (fission yeast)	yta12	NP_596797.1
Neurospora crassa	NCU01479	XP_956756.1
Danio rerio (zebrafish)	si:ch1073-174d20.2	XP_005163264.1
Rattus norvegicus (Norway rat)	Afg3l1	NP_001101926.3
Eremothecium gossypii	AGOS_ABL041W	NP_982906.2
Xenopus tropicalis (tropical clawed frog)	afg3l1p	NP_001015906.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that replacement of the Yta10 TM2 domain abolishes membrane dislocation for only a subset of substrates, whereas replacement of the Yta12 TM2 domain impairs membrane dislocation for all tested substrates, suggesting different roles of the TM domains in each m-AAA protease subunit
Title: Molecular insights into the

Rca1p appears to regulate cellular metabolism in response to CO(2) availability in environments as diverse as the phagosome, yeast communities or liquid culture.
Title: The bZIP transcription factor Rca1p is a central regulator of a novel CO? sensing pathway in yeast.

Inactivation of either of the mitochondrial AAA proteases, Yme1 or Yta10/12, allows fermentative growth of hsc82 or hsp82 strains at 37 degrees C.
Title: Hsp90 and mitochondrial proteases Yme1 and Yta10/12 participate in ATP synthase assembly in Saccharomyces cerevisiae.

mechanism regarding how proteins are recognized and degraded by m-AAA proteases.
Title: Electron cryomicroscopy structure of a membrane-anchored mitochondrial AAA protease.

Results describe the localizations of the m-AAA protease, its subunits Yta10 and Yta12, and its substrate cytochrome c peroxidase (Ccp1) within yeast mitochondria.
Title: The m-AAA protease processes cytochrome c peroxidase preferentially at the inner boundary membrane of mitochondria.

The following YTA12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YTA12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04086	NM_001182589.1 Latest version!	Saccharomyces cerevisiae S288C m-AAA protease subunit YTA12 (YTA12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YTA12 ( NM_001182589.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013807.1 Saccharomyces cerevisiae S288C m-AAA protease subunit YTA12 (YTA12), partial mRNA.

Price & Availability↑

CloneID	OSi04086
Clone ID Related Accession (Same CDS sequence)	NM_001182589.1
Accession Version	NM_001182589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	m-AAA protease subunit YTA12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTTGCTAC TTTCTTGGTC AAGAATTGCT ACTAAGGTAG TTAGAAGGCC CGTTCGCTTC 
CGGTCCTATT ACGGCTTGAC CCATATCAAG AGTTTACATA CTCAATATCG GCTACTCAAT 
AGGCTGCAAG AGAATAAGAG CGGTAACAAG AACGAGGACA ATAACGAAGA CGCTAAACTT 
AACAAAGAAA TTCCCACAGA CGAAGAGGTT GAAGCAATAA GAAAACAAGT AGAAAAATAT 
ATCGAACAAA CTAAAAATAA TACTATACCG GCTAACTGGA AAGAACAGAA ACGCAAAATT 
GATGAAAGTA TTAGGCGTTT AGAAGATGCT GTATTGAAAC AAGAATCTAA CAGAATTCAA 
GAAGAAAGAA AGGAAAAAGA GGAGGAAAAT GGTCCTTCAA AAGCCAAGTC AAACAGAACA 
AAAGAGCAGG GCTACTTTGA AGGTAACAAT AGCAGAAATA TTCCACCGCC ACCACCACCT 
CCTCCCCCAA AACCACCTCT AAATGATCCT AGTAATCCTG TATCGAAAAA TGTTAACTTA 
TTCCAGATAG GACTAACTTT TTTCCTCCTT TCGTTTTTAT TGGATCTCCT CAACAGTTTG 
GAAGAGCAAA GTGAGATAAC GTGGCAGGAT TTTAGAGAGA AACTATTGGC AAAAGGTTAT 
GTTGCGAAGT TGATTGTTGT CAACAAGTCC ATGGTGAAAG TGATGTTAAA TGACAACGGA 
AAAAACCAAG CCGACAATTA CGGTCGCAAC TTCTATTATT TCACAATTGG TTCAATTGAT 
AGCTTCGAAC ACAAACTTCA GAAAGCACAG GACGAACTGG ACATTGATAA GGATTTCAGA 
ATACCTGTAC TATATGTTCA AGAAGGCAAT TGGGCTAAAG CTATGTTCCA AATTTTGCCA 
ACTGTTTTAA TGATTGCAGG TATTATTTGG TTGACAAGAA GATCGGCACA AGCAGCGGGC 
GGTAGCCGTG GAGGCATATT TGGTCTGAGT CGTTCTAAGG CTAAAAAATT TAACACTGAA 
ACAGACGTTA AGATCAAGTT CAAGGACGTT GCCGGATGCG ATGAGGCTAA GGAAGAAATT 
ATGGAGTTTG TTAGCTTCTT GAAAGAACCT TCTCGTTATG AAAAAATGGG AGCTAAAATT 
CCTAGAGGTG CGATTCTATC TGGTCCACCA GGTACCGGTA AAACTTTGCT TGCAAAGGCA 
ACAGCAGGTG AAGCCGGTGT TCCGTTTTAT TTTGTTTCTG GTTCAGAATT TGTAGAAATG 
TTTGTTGGTG TTGGTGCAGC AAGAGTAAGA GATTTGTTCA AAACTGCAAG AGAAAATGCA 
CCCTCAATTG TGTTTATTGA CGAAATTGAT GCTATAGGAA AAGCAAGACA AAAGGGTAAT 
TTTTCAGGTG CCAATGATGA GAGAGAGAAC ACGCTAAATC AAATGTTAGT AGAAATGGAT 
GGTTTCACTC CAGCTGACCA CGTTGTTGTT TTAGCTGGTA CCAATAGACC CGATATTCTC 
GATAAAGCAT TATTAAGGCC TGGTAGATTT GACAGGCATA TCAATATCGA CAAGCCCGAA 
CTTGAGGGTA GAAAAGCCAT TTTTGCTGTT CATTTACATC ATTTGAAACT TGCCGGGGAA 
ATTTTTGATT TGAAAAATAG ATTAGCGGCT TTGACGCCAG GCTTTTCTGG TGCTGATATT 
GCCAACGTTT GTAACGAGGC CGCCTTGATT GCTGCTAGAA GTGATGAAGA TGCAGTAAAA 
TTGAATCATT TTGAACAAGC TATCGAAAGA GTCATCGGTG GAGTGGAACG CAAATCCAAG 
CTACTCTCTC CCGAAGAGAA GAAAGTAGTG GCTTACCACG AGGCTGGTCA TGCTGTATGT 
GGATGGTACT TAAAATACGC AGATCCTCTT CTGAAAGTCA GTATTATTCC ACGTGGCCAG 
GGCGCCCTTG GTTATGCACA GTATTTGCCA GGTGATATCT TCTTACTAAC TGAACAGCAA 
CTTAAGGACA GAATGACCAT GTCTCTGGGT GGAAGAGTTT CTGAAGAATT ACATTTCCCA 
TCAGTTACAA GTGGCGCATC TGATGACTTC AAAAAAGTTA CTAGCATGGC CACGGCAATG 
GTCACAGAAT TAGGCATGAG TGATAAAATT GGCTGGGTCA ATTACCAGAA GAGAGATGAT 
AGCGATTTGA CAAAACCATT TTCGGACGAG ACAGGTGACA TTATTGATTC CGAAGTATAC 
AGAATCGTCC AAGAATGCCA TGACAGATGT ACAAAGCTGC TTAAAGAAAA AGCAGAAGAT 
GTAGAGAAGA TTGCTCAAGT ACTACTGAAA AAAGAAGTTT TGACAAGAGA AGATATGATT 
GATTTACTTG GTAAGCGCCC TTTCCCAGAA AGGAATGATG CTTTTGATAA GTACTTGAAC 
GATTACGAAA CGGAAAAAAT CAGGAAGGAA GAAGAAAAAA ACGAAAAACG TAATGAGCCT 
AAGCCATCTA CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013807.1
CDS	1..2478
Translation	MLLLSWSRIATKVVRRPVRFRSYYGLTHIKSLHTQYRLLNRLQENKSGNKNEDNNEDAKLNKEIPTDEEVEAIRKQVEKYIEQTKNNTIPANWKEQKRKIDESIRRLEDAVLKQESNRIQEERKEKEEENGPSKAKSNRTKEQGYFEGNNSRNIPPPPPPPPPKPPLNDPSNPVSKNVNLFQIGLTFFLLSFLLDLLNSLEEQSEITWQDFREKLLAKGYVAKLIVVNKSMVKVMLNDNGKNQADNYGRNFYYFTIGSIDSFEHKLQKAQDELDIDKDFRIPVLYVQEGNWAKAMFQILPTVLMIAGIIWLTRRSAQAAGGSRGGIFGLSRSKAKKFNTETDVKIKFKDVAGCDEAKEEIMEFVSFLKEPSRYEKMGAKIPRGAILSGPPGTGKTLLAKATAGEAGVPFYFVSGSEFVEMFVGVGAARVRDLFKTARENAPSIVFIDEIDAIGKARQKGNFSGANDERENTLNQMLVEMDGFTPADHVVVLAGTNRPDILDKALLRPGRFDRHINIDKPELEGRKAIFAVHLHHLKLAGEIFDLKNRLAALTPGFSGADIANVCNEAALIAARSDEDAVKLNHFEQAIERVIGGVERKSKLLSPEEKKVVAYHEAGHAVCGWYLKYADPLLKVSIIPRGQGALGYAQYLPGDIFLLTEQQLKDRMTMSLGGRVSEELHFPSVTSGASDDFKKVTSMATAMVTELGMSDKIGWVNYQKRDDSDLTKPFSDETGDIIDSEVYRIVQECHDRCTKLLKEKAEDVEKIAQVLLKKEVLTREDMIDLLGKRPFPERNDAFDKYLNDYETEKIRKEEEKNEKRNEPKPSTN

Target ORF information:

RefSeq Version	NM_001182589.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C m-AAA protease subunit YTA12 (YTA12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182589.1

ORF Insert Sequence:

ATGTTGCTAC TTTCTTGGTC AAGAATTGCT ACTAAGGTAG TTAGAAGGCC CGTTCGCTTC 
CGGTCCTATT ACGGCTTGAC CCATATCAAG AGTTTACATA CTCAATATCG GCTACTCAAT 
AGGCTGCAAG AGAATAAGAG CGGTAACAAG AACGAGGACA ATAACGAAGA CGCTAAACTT 
AACAAAGAAA TTCCCACAGA CGAAGAGGTT GAAGCAATAA GAAAACAAGT AGAAAAATAT 
ATCGAACAAA CTAAAAATAA TACTATACCG GCTAACTGGA AAGAACAGAA ACGCAAAATT 
GATGAAAGTA TTAGGCGTTT AGAAGATGCT GTATTGAAAC AAGAATCTAA CAGAATTCAA 
GAAGAAAGAA AGGAAAAAGA GGAGGAAAAT GGTCCTTCAA AAGCCAAGTC AAACAGAACA 
AAAGAGCAGG GCTACTTTGA AGGTAACAAT AGCAGAAATA TTCCACCGCC ACCACCACCT 
CCTCCCCCAA AACCACCTCT AAATGATCCT AGTAATCCTG TATCGAAAAA TGTTAACTTA 
TTCCAGATAG GACTAACTTT TTTCCTCCTT TCGTTTTTAT TGGATCTCCT CAACAGTTTG 
GAAGAGCAAA GTGAGATAAC GTGGCAGGAT TTTAGAGAGA AACTATTGGC AAAAGGTTAT 
GTTGCGAAGT TGATTGTTGT CAACAAGTCC ATGGTGAAAG TGATGTTAAA TGACAACGGA 
AAAAACCAAG CCGACAATTA CGGTCGCAAC TTCTATTATT TCACAATTGG TTCAATTGAT 
AGCTTCGAAC ACAAACTTCA GAAAGCACAG GACGAACTGG ACATTGATAA GGATTTCAGA 
ATACCTGTAC TATATGTTCA AGAAGGCAAT TGGGCTAAAG CTATGTTCCA AATTTTGCCA 
ACTGTTTTAA TGATTGCAGG TATTATTTGG TTGACAAGAA GATCGGCACA AGCAGCGGGC 
GGTAGCCGTG GAGGCATATT TGGTCTGAGT CGTTCTAAGG CTAAAAAATT TAACACTGAA 
ACAGACGTTA AGATCAAGTT CAAGGACGTT GCCGGATGCG ATGAGGCTAA GGAAGAAATT 
ATGGAGTTTG TTAGCTTCTT GAAAGAACCT TCTCGTTATG AAAAAATGGG AGCTAAAATT 
CCTAGAGGTG CGATTCTATC TGGTCCACCA GGTACCGGTA AAACTTTGCT TGCAAAGGCA 
ACAGCAGGTG AAGCCGGTGT TCCGTTTTAT TTTGTTTCTG GTTCAGAATT TGTAGAAATG 
TTTGTTGGTG TTGGTGCAGC AAGAGTAAGA GATTTGTTCA AAACTGCAAG AGAAAATGCA 
CCCTCAATTG TGTTTATTGA CGAAATTGAT GCTATAGGAA AAGCAAGACA AAAGGGTAAT 
TTTTCAGGTG CCAATGATGA GAGAGAGAAC ACGCTAAATC AAATGTTAGT AGAAATGGAT 
GGTTTCACTC CAGCTGACCA CGTTGTTGTT TTAGCTGGTA CCAATAGACC CGATATTCTC 
GATAAAGCAT TATTAAGGCC TGGTAGATTT GACAGGCATA TCAATATCGA CAAGCCCGAA 
CTTGAGGGTA GAAAAGCCAT TTTTGCTGTT CATTTACATC ATTTGAAACT TGCCGGGGAA 
ATTTTTGATT TGAAAAATAG ATTAGCGGCT TTGACGCCAG GCTTTTCTGG TGCTGATATT 
GCCAACGTTT GTAACGAGGC CGCCTTGATT GCTGCTAGAA GTGATGAAGA TGCAGTAAAA 
TTGAATCATT TTGAACAAGC TATCGAAAGA GTCATCGGTG GAGTGGAACG CAAATCCAAG 
CTACTCTCTC CCGAAGAGAA GAAAGTAGTG GCTTACCACG AGGCTGGTCA TGCTGTATGT 
GGATGGTACT TAAAATACGC AGATCCTCTT CTGAAAGTCA GTATTATTCC ACGTGGCCAG 
GGCGCCCTTG GTTATGCACA GTATTTGCCA GGTGATATCT TCTTACTAAC TGAACAGCAA 
CTTAAGGACA GAATGACCAT GTCTCTGGGT GGAAGAGTTT CTGAAGAATT ACATTTCCCA 
TCAGTTACAA GTGGCGCATC TGATGACTTC AAAAAAGTTA CTAGCATGGC CACGGCAATG 
GTCACAGAAT TAGGCATGAG TGATAAAATT GGCTGGGTCA ATTACCAGAA GAGAGATGAT 
AGCGATTTGA CAAAACCATT TTCGGACGAG ACAGGTGACA TTATTGATTC CGAAGTATAC 
AGAATCGTCC AAGAATGCCA TGACAGATGT ACAAAGCTGC TTAAAGAAAA AGCAGAAGAT 
GTAGAGAAGA TTGCTCAAGT ACTACTGAAA AAAGAAGTTT TGACAAGAGA AGATATGATT 
GATTTACTTG GTAAGCGCCC TTTCCCAGAA AGGAATGATG CTTTTGATAA GTACTTGAAC 
GATTACGAAA CGGAAAAAAT CAGGAAGGAA GAAGAAAAAA ACGAAAAACG TAATGAGCCT 
AAGCCATCTA CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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YTA12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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