LOC532875 cDNA ORF clone, Bos taurus(cattle)

The following LOC532875 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC532875 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa09250 XM_002694867.5
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09250 XM_612083.7
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09251 XM_015467325.1
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09251 XM_015475792.2
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09251 XM_024978928.1
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09252 XM_005192682.1
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09252 XM_024978929.1
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09252 XM_005218788.2
Latest version!
Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa09251 XM_015475792.1 Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09250 XM_002694867.4 Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09252 XM_005218788.1 Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa09250
    Clone ID Related Accession (Same CDS sequence) XM_612083.7 , XM_002694867.4 , XM_002694867.5
    Accession Version XM_612083.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_612083.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCGCAGC GGCTGGTGGC GGCGGCCGGC AGCAGGGCGG TGGTGGGGCC GCTCGCCTTG 
    CTGTGGCGGT GCGGCCTTGG CCCACGAGGG GGCAGGACGT TCGCGGCAGG CGCTCGGGCG
    CTCGGAACCA CTCAGCAGTG TCTCCAGGGA ATCCGCTTTC AGCAGTCGGC TCTGCAGATA
    AGACTGGCGT CAGTTTCCCA TTGGCTGTGT TCAAAACCAC CCAAAGGAAC TAGTGAACCA
    AAGGATGCTG GTCCTGGAGC GGACGGAGGG AAGAGAGGAG GAAAGCGGGA TGACTTTGCC
    TGGTGGAAAC GAATGCAGAA GGGGAAGGTG CCCTGGGACG ACAAGGATCT CCGCAGTCTG
    GCCGTGCTGG GGGCAGGTGT CGTTGCAGGA TTTCTCTACT TCTATTTCCG AGATCCTGGG
    AAGGAAATCA CTTGGAAGCA CTTCGTGCAG TATTACCTGG CGAGAGGTCT GGTGGATCGG
    CTGGAAGTGG TGAATAAACA GTTCGTGCGT GTTATTCCTG CCCCTGGGAC ATCACTCGAG
    AAGTATGCGT GGTTCAGCAT TGGCAGTGTG GACACCTTCG AGCGCAACCT GGAGACCGCC
    CAATGGGAGC TAGGCATCGA GCCCCCCAAC CAGACGGCCG TGGTCTACAC CACCGAGAGC
    GATGGGACTT TCTTGCGAAG CCTGGTGCCC ACCCTTCTCC TGATTGGCAT CTTCCTCTAT
    GCCATGAGGA GGGGTCCAAT GGGGGCTGGG CGTGGTGGGC GAGGAGGGCG CCTCTTCGGC
    ATTGGTGAGA CAATAGCCAA GATCATCAAA GATGACATCG GTGTGCGGTT TGCAGACGTG
    GCTGGTTGCG AAGAAGCCAA GCTGGAGATC ATGGAGTTTG TGAATTTCCT GAAGAACCCC
    AAACAGTACC AGGACCTTGG GGCCAAAATT CCAAAGGGAG CCCTGCTCAC AGGTCCTCCC
    GGTACCGGCA AGACGCTTCT CGCCAAGGCA ACCGCAGGGG AGGCTGGTGT GCCCTTCATC
    ACCGTGAATG GGTCTGAGTT CCTGGAGATG TTTGTTGGTG TTGGCCCAGC TCGGGTTCGT
    GACATGTTTG CACTGGCCCG GAAAAACGCT CCCTGTATCT TGTTTGTGGA CGAGATTGAT
    GCAATTGGCC GGAAGCGTGG CCGTGGGCAC TTTGGAGGCC AGAGTGAGCA GGAGAACACC
    CTGAACCAGC TGCTCGTGGA GATGGACGGA TTCAACTCTA CCACGAACGT TGTCGTGCTG
    GCTGGAACCA ACCGCCCTGA CGTCCTTGAC CCAGCTCTGA TGCGGCCGGG CCGCTTCGAC
    CGTCAGATTT ACATTGGGCC CCCGGACATC AAAGGCAGGT CGTCTATCTT CAGGGTCCAC
    CTGCGTCCAC TCAAGTTGGA CGAGAGCCTC AGCAAGGATG CTCTGGCAAG GAAGCTTGCG
    GCCCTCACTC CAGGCTTCAC AGGTGCTGAT ATTTCTAATG TTTGCAATGA AGCCGCCCTG
    ATCGCCGCTC GCCACCTAAA CCCCTCTGTC GGGGAGAAGC ACTTCGAGCA GGCCATCGAG
    AGGGTCATTG GAGGCCTTGA GAAGAAGACA CAGGTCCTGC AGCCTGGCGA GAAGATGACA
    GTGGCCTACC ACGAGGCTGG CCATGCGGTG GTGGGCTGGT TCCTGGAACA CGCAGACCCC
    CTGCTCAAGG TGTCCATCGT CCCCCGGGGC AAGGGGCTCG GCTATGCCCA GTGTCTGCCC
    AGGGAGCAGT ACCTGTACAC ACGGGAGCAG CTCTTTGACC GCATGTGTGC CATGCTGGGC
    GGCCGCGTGG CTGAGCAGCT GTTCTTCGGG CGGGTCACCA CGGGGGCCCA GGATGACCTG
    AGGAAGGTCA CCCAGAGCGC CTATGCCCAG ATTGTGCAGT TCGGGATGAG CGAGAAGCTG
    GGCCAGGTGT CCTTCGATCT CCCGCGGCCA GGTGAGGCGC TGGTGGAGAA ACCGTTCAGC
    GAGGCCACGG CCCAGCTCAT AGACGAGGAG GTGCGGCGGC TCATTGGTTC TGCGCATGCG
    CGCACCCTGG ACCTGCTCAC GCGCTGCCGC GAGCAGGTGG ACAAGGTGGG CAGGCGGCTG
    CTGGAGAAGG AGGTGCTGGA GCGGGCGGAC ATGGTGGAGC TGCTCGGGCC GCGGCCGTTC
    GCCGAGAAGG TCACCTACGA GGAGCTCGTG GAGGGCACCG GCGGCCTGGA GGAGGACACG
    GCCCTTCCCG AGGGTCTGCA GGGCTGGCGT GGGGGGCCCA CTGCAGGAGA GCCCAGCCTA
    GCACCTCCGC CTGGAGAGCA GCCTCTGGGA CCCCAGGAAG CAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_612083.5
    CDS42..2369
    Translation

    Target ORF information:

    RefSeq Version XM_612083.7
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_612083.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCGCAGC GGCTGGTGGC GGCGGCCGGC AGCAGGGCGG TGGTGGGGCC GCTCGCCTTG 
    CTGTGGCGGT GCGGCCTTGG CCCACGAGGG GGCAGGACGT TCGCGGCAGG CGCTCGGGCG
    CTCGGAACCA CTCAGCAGTG TCTCCAGGGA ATCCGCTTTC AGCAGTCGGC TCTGCAGATA
    AGACTGGCGT CAGTTTCCCA TTGGCTGTGT TCAAAACCAC CCAAAGGAAC TAGTGAACCA
    AAGGATGCTG GTCCTGGAGC GGACGGAGGG AAGAGAGGAG GAAAGCGGGA TGACTTTGCC
    TGGTGGAAAC GAATGCAGAA GGGGAAGGTG CCCTGGGACG ACAAGGATCT CCGCAGTCTG
    GCCGTGCTGG GGGCAGGTGT CGTTGCAGGA TTTCTCTACT TCTATTTCCG AGATCCTGGG
    AAGGAAATCA CTTGGAAGCA CTTCGTGCAG TATTACCTGG CGAGAGGTCT GGTGGATCGG
    CTGGAAGTGG TGAATAAACA GTTCGTGCGT GTTATTCCTG CCCCTGGGAC ATCACTCGAG
    AAGTATGCGT GGTTCAGCAT TGGCAGTGTG GACACCTTCG AGCGCAACCT GGAGACCGCC
    CAATGGGAGC TAGGCATCGA GCCCCCCAAC CAGACGGCCG TGGTCTACAC CACCGAGAGC
    GATGGGACTT TCTTGCGAAG CCTGGTGCCC ACCCTTCTCC TGATTGGCAT CTTCCTCTAT
    GCCATGAGGA GGGGTCCAAT GGGGGCTGGG CGTGGTGGGC GAGGAGGGCG CCTCTTCGGC
    ATTGGTGAGA CAATAGCCAA GATCATCAAA GATGACATCG GTGTGCGGTT TGCAGACGTG
    GCTGGTTGCG AAGAAGCCAA GCTGGAGATC ATGGAGTTTG TGAATTTCCT GAAGAACCCC
    AAACAGTACC AGGACCTTGG GGCCAAAATT CCAAAGGGAG CCCTGCTCAC AGGTCCTCCC
    GGTACCGGCA AGACGCTTCT CGCCAAGGCA ACCGCAGGGG AGGCTGGTGT GCCCTTCATC
    ACCGTGAATG GGTCTGAGTT CCTGGAGATG TTTGTTGGTG TTGGCCCAGC TCGGGTTCGT
    GACATGTTTG CACTGGCCCG GAAAAACGCT CCCTGTATCT TGTTTGTGGA CGAGATTGAT
    GCAATTGGCC GGAAGCGTGG CCGTGGGCAC TTTGGAGGCC AGAGTGAGCA GGAGAACACC
    CTGAACCAGC TGCTCGTGGA GATGGACGGA TTCAACTCTA CCACGAACGT TGTCGTGCTG
    GCTGGAACCA ACCGCCCTGA CGTCCTTGAC CCAGCTCTGA TGCGGCCGGG CCGCTTCGAC
    CGTCAGATTT ACATTGGGCC CCCGGACATC AAAGGCAGGT CGTCTATCTT CAGGGTCCAC
    CTGCGTCCAC TCAAGTTGGA CGAGAGCCTC AGCAAGGATG CTCTGGCAAG GAAGCTTGCG
    GCCCTCACTC CAGGCTTCAC AGGTGCTGAT ATTTCTAATG TTTGCAATGA AGCCGCCCTG
    ATCGCCGCTC GCCACCTAAA CCCCTCTGTC GGGGAGAAGC ACTTCGAGCA GGCCATCGAG
    AGGGTCATTG GAGGCCTTGA GAAGAAGACA CAGGTCCTGC AGCCTGGCGA GAAGATGACA
    GTGGCCTACC ACGAGGCTGG CCATGCGGTG GTGGGCTGGT TCCTGGAACA CGCAGACCCC
    CTGCTCAAGG TGTCCATCGT CCCCCGGGGC AAGGGGCTCG GCTATGCCCA GTGTCTGCCC
    AGGGAGCAGT ACCTGTACAC ACGGGAGCAG CTCTTTGACC GCATGTGTGC CATGCTGGGC
    GGCCGCGTGG CTGAGCAGCT GTTCTTCGGG CGGGTCACCA CGGGGGCCCA GGATGACCTG
    AGGAAGGTCA CCCAGAGCGC CTATGCCCAG ATTGTGCAGT TCGGGATGAG CGAGAAGCTG
    GGCCAGGTGT CCTTCGATCT CCCGCGGCCA GGTGAGGCGC TGGTGGAGAA ACCGTTCAGC
    GAGGCCACGG CCCAGCTCAT AGACGAGGAG GTGCGGCGGC TCATTGGTTC TGCGCATGCG
    CGCACCCTGG ACCTGCTCAC GCGCTGCCGC GAGCAGGTGG ACAAGGTGGG CAGGCGGCTG
    CTGGAGAAGG AGGTGCTGGA GCGGGCGGAC ATGGTGGAGC TGCTCGGGCC GCGGCCGTTC
    GCCGAGAAGG TCACCTACGA GGAGCTCGTG GAGGGCACCG GCGGCCTGGA GGAGGACACG
    GCCCTTCCCG AGGGTCTGCA GGGCTGGCGT GGGGGGCCCA CTGCAGGAGA GCCCAGCCTA
    GCACCTCCGC CTGGAGAGCA GCCTCTGGGA CCCCAGGAAG CAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09250
    Clone ID Related Accession (Same CDS sequence) XM_612083.7 , XM_002694867.4 , XM_002694867.5
    Accession Version XM_002694867.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCGCAGC GGCTGGTGGC GGCGGCCGGC AGCAGGGCGG TGGTGGGGCC GCTCGCCTTG 
    CTGTGGCGGT GCGGCCTTGG CCCACGAGGG GGCAGGACGT TCGCGGCAGG CGCTCGGGCG
    CTCGGAACCA CTCAGCAGTG TCTCCAGGGA ATCCGCTTTC AGCAGTCGGC TCTGCAGATA
    AGACTGGCGT CAGTTTCCCA TTGGCTGTGT TCAAAACCAC CCAAAGGAAC TAGTGAACCA
    AAGGATGCTG GTCCTGGAGC GGACGGAGGG AAGAGAGGAG GAAAGCGGGA TGACTTTGCC
    TGGTGGAAAC GAATGCAGAA GGGGAAGGTG CCCTGGGACG ACAAGGATCT CCGCAGTCTG
    GCCGTGCTGG GGGCAGGTGT CGTTGCAGGA TTTCTCTACT TCTATTTCCG AGATCCTGGG
    AAGGAAATCA CTTGGAAGCA CTTCGTGCAG TATTACCTGG CGAGAGGTCT GGTGGATCGG
    CTGGAAGTGG TGAATAAACA GTTCGTGCGT GTTATTCCTG CCCCTGGGAC ATCACTCGAG
    AAGTATGCGT GGTTCAGCAT TGGCAGTGTG GACACCTTCG AGCGCAACCT GGAGACCGCC
    CAATGGGAGC TAGGCATCGA GCCCCCCAAC CAGACGGCCG TGGTCTACAC CACCGAGAGC
    GATGGGACTT TCTTGCGAAG CCTGGTGCCC ACCCTTCTCC TGATTGGCAT CTTCCTCTAT
    GCCATGAGGA GGGGTCCAAT GGGGGCTGGG CGTGGTGGGC GAGGAGGGCG CCTCTTCGGC
    ATTGGTGAGA CAATAGCCAA GATCATCAAA GATGACATCG GTGTGCGGTT TGCAGACGTG
    GCTGGTTGCG AAGAAGCCAA GCTGGAGATC ATGGAGTTTG TGAATTTCCT GAAGAACCCC
    AAACAGTACC AGGACCTTGG GGCCAAAATT CCAAAGGGAG CCCTGCTCAC AGGTCCTCCC
    GGTACCGGCA AGACGCTTCT CGCCAAGGCA ACCGCAGGGG AGGCTGGTGT GCCCTTCATC
    ACCGTGAATG GGTCTGAGTT CCTGGAGATG TTTGTTGGTG TTGGCCCAGC TCGGGTTCGT
    GACATGTTTG CACTGGCCCG GAAAAACGCT CCCTGTATCT TGTTTGTGGA CGAGATTGAT
    GCAATTGGCC GGAAGCGTGG CCGTGGGCAC TTTGGAGGCC AGAGTGAGCA GGAGAACACC
    CTGAACCAGC TGCTCGTGGA GATGGACGGA TTCAACTCTA CCACGAACGT TGTCGTGCTG
    GCTGGAACCA ACCGCCCTGA CGTCCTTGAC CCAGCTCTGA TGCGGCCGGG CCGCTTCGAC
    CGTCAGATTT ACATTGGGCC CCCGGACATC AAAGGCAGGT CGTCTATCTT CAGGGTCCAC
    CTGCGTCCAC TCAAGTTGGA CGAGAGCCTC AGCAAGGATG CTCTGGCAAG GAAGCTTGCG
    GCCCTCACTC CAGGCTTCAC AGGTGCTGAT ATTTCTAATG TTTGCAATGA AGCCGCCCTG
    ATCGCCGCTC GCCACCTAAA CCCCTCTGTC GGGGAGAAGC ACTTCGAGCA GGCCATCGAG
    AGGGTCATTG GAGGCCTTGA GAAGAAGACA CAGGTCCTGC AGCCTGGCGA GAAGATGACA
    GTGGCCTACC ACGAGGCTGG CCATGCGGTG GTGGGCTGGT TCCTGGAACA CGCAGACCCC
    CTGCTCAAGG TGTCCATCGT CCCCCGGGGC AAGGGGCTCG GCTATGCCCA GTGTCTGCCC
    AGGGAGCAGT ACCTGTACAC ACGGGAGCAG CTCTTTGACC GCATGTGTGC CATGCTGGGC
    GGCCGCGTGG CTGAGCAGCT GTTCTTCGGG CGGGTCACCA CGGGGGCCCA GGATGACCTG
    AGGAAGGTCA CCCAGAGCGC CTATGCCCAG ATTGTGCAGT TCGGGATGAG CGAGAAGCTG
    GGCCAGGTGT CCTTCGATCT CCCGCGGCCA GGTGAGGCGC TGGTGGAGAA ACCGTTCAGC
    GAGGCCACGG CCCAGCTCAT AGACGAGGAG GTGCGGCGGC TCATTGGTTC TGCGCATGCG
    CGCACCCTGG ACCTGCTCAC GCGCTGCCGC GAGCAGGTGG ACAAGGTGGG CAGGCGGCTG
    CTGGAGAAGG AGGTGCTGGA GCGGGCGGAC ATGGTGGAGC TGCTCGGGCC GCGGCCGTTC
    GCCGAGAAGG TCACCTACGA GGAGCTCGTG GAGGGCACCG GCGGCCTGGA GGAGGACACG
    GCCCTTCCCG AGGGTCTGCA GGGCTGGCGT GGGGGGCCCA CTGCAGGAGA GCCCAGCCTA
    GCACCTCCGC CTGGAGAGCA GCCTCTGGGA CCCCAGGAAG CAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694913.2
    CDS42..2369
    Translation

    Target ORF information:

    RefSeq Version XM_002694867.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694867.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCGCAGC GGCTGGTGGC GGCGGCCGGC AGCAGGGCGG TGGTGGGGCC GCTCGCCTTG 
    CTGTGGCGGT GCGGCCTTGG CCCACGAGGG GGCAGGACGT TCGCGGCAGG CGCTCGGGCG
    CTCGGAACCA CTCAGCAGTG TCTCCAGGGA ATCCGCTTTC AGCAGTCGGC TCTGCAGATA
    AGACTGGCGT CAGTTTCCCA TTGGCTGTGT TCAAAACCAC CCAAAGGAAC TAGTGAACCA
    AAGGATGCTG GTCCTGGAGC GGACGGAGGG AAGAGAGGAG GAAAGCGGGA TGACTTTGCC
    TGGTGGAAAC GAATGCAGAA GGGGAAGGTG CCCTGGGACG ACAAGGATCT CCGCAGTCTG
    GCCGTGCTGG GGGCAGGTGT CGTTGCAGGA TTTCTCTACT TCTATTTCCG AGATCCTGGG
    AAGGAAATCA CTTGGAAGCA CTTCGTGCAG TATTACCTGG CGAGAGGTCT GGTGGATCGG
    CTGGAAGTGG TGAATAAACA GTTCGTGCGT GTTATTCCTG CCCCTGGGAC ATCACTCGAG
    AAGTATGCGT GGTTCAGCAT TGGCAGTGTG GACACCTTCG AGCGCAACCT GGAGACCGCC
    CAATGGGAGC TAGGCATCGA GCCCCCCAAC CAGACGGCCG TGGTCTACAC CACCGAGAGC
    GATGGGACTT TCTTGCGAAG CCTGGTGCCC ACCCTTCTCC TGATTGGCAT CTTCCTCTAT
    GCCATGAGGA GGGGTCCAAT GGGGGCTGGG CGTGGTGGGC GAGGAGGGCG CCTCTTCGGC
    ATTGGTGAGA CAATAGCCAA GATCATCAAA GATGACATCG GTGTGCGGTT TGCAGACGTG
    GCTGGTTGCG AAGAAGCCAA GCTGGAGATC ATGGAGTTTG TGAATTTCCT GAAGAACCCC
    AAACAGTACC AGGACCTTGG GGCCAAAATT CCAAAGGGAG CCCTGCTCAC AGGTCCTCCC
    GGTACCGGCA AGACGCTTCT CGCCAAGGCA ACCGCAGGGG AGGCTGGTGT GCCCTTCATC
    ACCGTGAATG GGTCTGAGTT CCTGGAGATG TTTGTTGGTG TTGGCCCAGC TCGGGTTCGT
    GACATGTTTG CACTGGCCCG GAAAAACGCT CCCTGTATCT TGTTTGTGGA CGAGATTGAT
    GCAATTGGCC GGAAGCGTGG CCGTGGGCAC TTTGGAGGCC AGAGTGAGCA GGAGAACACC
    CTGAACCAGC TGCTCGTGGA GATGGACGGA TTCAACTCTA CCACGAACGT TGTCGTGCTG
    GCTGGAACCA ACCGCCCTGA CGTCCTTGAC CCAGCTCTGA TGCGGCCGGG CCGCTTCGAC
    CGTCAGATTT ACATTGGGCC CCCGGACATC AAAGGCAGGT CGTCTATCTT CAGGGTCCAC
    CTGCGTCCAC TCAAGTTGGA CGAGAGCCTC AGCAAGGATG CTCTGGCAAG GAAGCTTGCG
    GCCCTCACTC CAGGCTTCAC AGGTGCTGAT ATTTCTAATG TTTGCAATGA AGCCGCCCTG
    ATCGCCGCTC GCCACCTAAA CCCCTCTGTC GGGGAGAAGC ACTTCGAGCA GGCCATCGAG
    AGGGTCATTG GAGGCCTTGA GAAGAAGACA CAGGTCCTGC AGCCTGGCGA GAAGATGACA
    GTGGCCTACC ACGAGGCTGG CCATGCGGTG GTGGGCTGGT TCCTGGAACA CGCAGACCCC
    CTGCTCAAGG TGTCCATCGT CCCCCGGGGC AAGGGGCTCG GCTATGCCCA GTGTCTGCCC
    AGGGAGCAGT ACCTGTACAC ACGGGAGCAG CTCTTTGACC GCATGTGTGC CATGCTGGGC
    GGCCGCGTGG CTGAGCAGCT GTTCTTCGGG CGGGTCACCA CGGGGGCCCA GGATGACCTG
    AGGAAGGTCA CCCAGAGCGC CTATGCCCAG ATTGTGCAGT TCGGGATGAG CGAGAAGCTG
    GGCCAGGTGT CCTTCGATCT CCCGCGGCCA GGTGAGGCGC TGGTGGAGAA ACCGTTCAGC
    GAGGCCACGG CCCAGCTCAT AGACGAGGAG GTGCGGCGGC TCATTGGTTC TGCGCATGCG
    CGCACCCTGG ACCTGCTCAC GCGCTGCCGC GAGCAGGTGG ACAAGGTGGG CAGGCGGCTG
    CTGGAGAAGG AGGTGCTGGA GCGGGCGGAC ATGGTGGAGC TGCTCGGGCC GCGGCCGTTC
    GCCGAGAAGG TCACCTACGA GGAGCTCGTG GAGGGCACCG GCGGCCTGGA GGAGGACACG
    GCCCTTCCCG AGGGTCTGCA GGGCTGGCGT GGGGGGCCCA CTGCAGGAGA GCCCAGCCTA
    GCACCTCCGC CTGGAGAGCA GCCTCTGGGA CCCCAGGAAG CAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09250
    Clone ID Related Accession (Same CDS sequence) XM_612083.7 , XM_002694867.4 , XM_002694867.5
    Accession Version XM_002694867.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694867.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCGCAGC GGCTGGTGGC GGCGGCCGGC AGCAGGGCGG TGGTGGGGCC GCTCGCCTTG 
    CTGTGGCGGT GCGGCCTTGG CCCACGAGGG GGCAGGACGT TCGCGGCAGG CGCTCGGGCG
    CTCGGAACCA CTCAGCAGTG TCTCCAGGGA ATCCGCTTTC AGCAGTCGGC TCTGCAGATA
    AGACTGGCGT CAGTTTCCCA TTGGCTGTGT TCAAAACCAC CCAAAGGAAC TAGTGAACCA
    AAGGATGCTG GTCCTGGAGC GGACGGAGGG AAGAGAGGAG GAAAGCGGGA TGACTTTGCC
    TGGTGGAAAC GAATGCAGAA GGGGAAGGTG CCCTGGGACG ACAAGGATCT CCGCAGTCTG
    GCCGTGCTGG GGGCAGGTGT CGTTGCAGGA TTTCTCTACT TCTATTTCCG AGATCCTGGG
    AAGGAAATCA CTTGGAAGCA CTTCGTGCAG TATTACCTGG CGAGAGGTCT GGTGGATCGG
    CTGGAAGTGG TGAATAAACA GTTCGTGCGT GTTATTCCTG CCCCTGGGAC ATCACTCGAG
    AAGTATGCGT GGTTCAGCAT TGGCAGTGTG GACACCTTCG AGCGCAACCT GGAGACCGCC
    CAATGGGAGC TAGGCATCGA GCCCCCCAAC CAGACGGCCG TGGTCTACAC CACCGAGAGC
    GATGGGACTT TCTTGCGAAG CCTGGTGCCC ACCCTTCTCC TGATTGGCAT CTTCCTCTAT
    GCCATGAGGA GGGGTCCAAT GGGGGCTGGG CGTGGTGGGC GAGGAGGGCG CCTCTTCGGC
    ATTGGTGAGA CAATAGCCAA GATCATCAAA GATGACATCG GTGTGCGGTT TGCAGACGTG
    GCTGGTTGCG AAGAAGCCAA GCTGGAGATC ATGGAGTTTG TGAATTTCCT GAAGAACCCC
    AAACAGTACC AGGACCTTGG GGCCAAAATT CCAAAGGGAG CCCTGCTCAC AGGTCCTCCC
    GGTACCGGCA AGACGCTTCT CGCCAAGGCA ACCGCAGGGG AGGCTGGTGT GCCCTTCATC
    ACCGTGAATG GGTCTGAGTT CCTGGAGATG TTTGTTGGTG TTGGCCCAGC TCGGGTTCGT
    GACATGTTTG CACTGGCCCG GAAAAACGCT CCCTGTATCT TGTTTGTGGA CGAGATTGAT
    GCAATTGGCC GGAAGCGTGG CCGTGGGCAC TTTGGAGGCC AGAGTGAGCA GGAGAACACC
    CTGAACCAGC TGCTCGTGGA GATGGACGGA TTCAACTCTA CCACGAACGT TGTCGTGCTG
    GCTGGAACCA ACCGCCCTGA CGTCCTTGAC CCAGCTCTGA TGCGGCCGGG CCGCTTCGAC
    CGTCAGATTT ACATTGGGCC CCCGGACATC AAAGGCAGGT CGTCTATCTT CAGGGTCCAC
    CTGCGTCCAC TCAAGTTGGA CGAGAGCCTC AGCAAGGATG CTCTGGCAAG GAAGCTTGCG
    GCCCTCACTC CAGGCTTCAC AGGTGCTGAT ATTTCTAATG TTTGCAATGA AGCCGCCCTG
    ATCGCCGCTC GCCACCTAAA CCCCTCTGTC GGGGAGAAGC ACTTCGAGCA GGCCATCGAG
    AGGGTCATTG GAGGCCTTGA GAAGAAGACA CAGGTCCTGC AGCCTGGCGA GAAGATGACA
    GTGGCCTACC ACGAGGCTGG CCATGCGGTG GTGGGCTGGT TCCTGGAACA CGCAGACCCC
    CTGCTCAAGG TGTCCATCGT CCCCCGGGGC AAGGGGCTCG GCTATGCCCA GTGTCTGCCC
    AGGGAGCAGT ACCTGTACAC ACGGGAGCAG CTCTTTGACC GCATGTGTGC CATGCTGGGC
    GGCCGCGTGG CTGAGCAGCT GTTCTTCGGG CGGGTCACCA CGGGGGCCCA GGATGACCTG
    AGGAAGGTCA CCCAGAGCGC CTATGCCCAG ATTGTGCAGT TCGGGATGAG CGAGAAGCTG
    GGCCAGGTGT CCTTCGATCT CCCGCGGCCA GGTGAGGCGC TGGTGGAGAA ACCGTTCAGC
    GAGGCCACGG CCCAGCTCAT AGACGAGGAG GTGCGGCGGC TCATTGGTTC TGCGCATGCG
    CGCACCCTGG ACCTGCTCAC GCGCTGCCGC GAGCAGGTGG ACAAGGTGGG CAGGCGGCTG
    CTGGAGAAGG AGGTGCTGGA GCGGGCGGAC ATGGTGGAGC TGCTCGGGCC GCGGCCGTTC
    GCCGAGAAGG TCACCTACGA GGAGCTCGTG GAGGGCACCG GCGGCCTGGA GGAGGACACG
    GCCCTTCCCG AGGGTCTGCA GGGCTGGCGT GGGGGGCCCA CTGCAGGAGA GCCCAGCCTA
    GCACCTCCGC CTGGAGAGCA GCCTCTGGGA CCCCAGGAAG CAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694913.2
    CDS46..2373
    Translation

    Target ORF information:

    RefSeq Version XM_002694867.5
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694867.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCGCAGC GGCTGGTGGC GGCGGCCGGC AGCAGGGCGG TGGTGGGGCC GCTCGCCTTG 
    CTGTGGCGGT GCGGCCTTGG CCCACGAGGG GGCAGGACGT TCGCGGCAGG CGCTCGGGCG
    CTCGGAACCA CTCAGCAGTG TCTCCAGGGA ATCCGCTTTC AGCAGTCGGC TCTGCAGATA
    AGACTGGCGT CAGTTTCCCA TTGGCTGTGT TCAAAACCAC CCAAAGGAAC TAGTGAACCA
    AAGGATGCTG GTCCTGGAGC GGACGGAGGG AAGAGAGGAG GAAAGCGGGA TGACTTTGCC
    TGGTGGAAAC GAATGCAGAA GGGGAAGGTG CCCTGGGACG ACAAGGATCT CCGCAGTCTG
    GCCGTGCTGG GGGCAGGTGT CGTTGCAGGA TTTCTCTACT TCTATTTCCG AGATCCTGGG
    AAGGAAATCA CTTGGAAGCA CTTCGTGCAG TATTACCTGG CGAGAGGTCT GGTGGATCGG
    CTGGAAGTGG TGAATAAACA GTTCGTGCGT GTTATTCCTG CCCCTGGGAC ATCACTCGAG
    AAGTATGCGT GGTTCAGCAT TGGCAGTGTG GACACCTTCG AGCGCAACCT GGAGACCGCC
    CAATGGGAGC TAGGCATCGA GCCCCCCAAC CAGACGGCCG TGGTCTACAC CACCGAGAGC
    GATGGGACTT TCTTGCGAAG CCTGGTGCCC ACCCTTCTCC TGATTGGCAT CTTCCTCTAT
    GCCATGAGGA GGGGTCCAAT GGGGGCTGGG CGTGGTGGGC GAGGAGGGCG CCTCTTCGGC
    ATTGGTGAGA CAATAGCCAA GATCATCAAA GATGACATCG GTGTGCGGTT TGCAGACGTG
    GCTGGTTGCG AAGAAGCCAA GCTGGAGATC ATGGAGTTTG TGAATTTCCT GAAGAACCCC
    AAACAGTACC AGGACCTTGG GGCCAAAATT CCAAAGGGAG CCCTGCTCAC AGGTCCTCCC
    GGTACCGGCA AGACGCTTCT CGCCAAGGCA ACCGCAGGGG AGGCTGGTGT GCCCTTCATC
    ACCGTGAATG GGTCTGAGTT CCTGGAGATG TTTGTTGGTG TTGGCCCAGC TCGGGTTCGT
    GACATGTTTG CACTGGCCCG GAAAAACGCT CCCTGTATCT TGTTTGTGGA CGAGATTGAT
    GCAATTGGCC GGAAGCGTGG CCGTGGGCAC TTTGGAGGCC AGAGTGAGCA GGAGAACACC
    CTGAACCAGC TGCTCGTGGA GATGGACGGA TTCAACTCTA CCACGAACGT TGTCGTGCTG
    GCTGGAACCA ACCGCCCTGA CGTCCTTGAC CCAGCTCTGA TGCGGCCGGG CCGCTTCGAC
    CGTCAGATTT ACATTGGGCC CCCGGACATC AAAGGCAGGT CGTCTATCTT CAGGGTCCAC
    CTGCGTCCAC TCAAGTTGGA CGAGAGCCTC AGCAAGGATG CTCTGGCAAG GAAGCTTGCG
    GCCCTCACTC CAGGCTTCAC AGGTGCTGAT ATTTCTAATG TTTGCAATGA AGCCGCCCTG
    ATCGCCGCTC GCCACCTAAA CCCCTCTGTC GGGGAGAAGC ACTTCGAGCA GGCCATCGAG
    AGGGTCATTG GAGGCCTTGA GAAGAAGACA CAGGTCCTGC AGCCTGGCGA GAAGATGACA
    GTGGCCTACC ACGAGGCTGG CCATGCGGTG GTGGGCTGGT TCCTGGAACA CGCAGACCCC
    CTGCTCAAGG TGTCCATCGT CCCCCGGGGC AAGGGGCTCG GCTATGCCCA GTGTCTGCCC
    AGGGAGCAGT ACCTGTACAC ACGGGAGCAG CTCTTTGACC GCATGTGTGC CATGCTGGGC
    GGCCGCGTGG CTGAGCAGCT GTTCTTCGGG CGGGTCACCA CGGGGGCCCA GGATGACCTG
    AGGAAGGTCA CCCAGAGCGC CTATGCCCAG ATTGTGCAGT TCGGGATGAG CGAGAAGCTG
    GGCCAGGTGT CCTTCGATCT CCCGCGGCCA GGTGAGGCGC TGGTGGAGAA ACCGTTCAGC
    GAGGCCACGG CCCAGCTCAT AGACGAGGAG GTGCGGCGGC TCATTGGTTC TGCGCATGCG
    CGCACCCTGG ACCTGCTCAC GCGCTGCCGC GAGCAGGTGG ACAAGGTGGG CAGGCGGCTG
    CTGGAGAAGG AGGTGCTGGA GCGGGCGGAC ATGGTGGAGC TGCTCGGGCC GCGGCCGTTC
    GCCGAGAAGG TCACCTACGA GGAGCTCGTG GAGGGCACCG GCGGCCTGGA GGAGGACACG
    GCCCTTCCCG AGGGTCTGCA GGGCTGGCGT GGGGGGCCCA CTGCAGGAGA GCCCAGCCTA
    GCACCTCCGC CTGGAGAGCA GCCTCTGGGA CCCCAGGAAG CAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09251
    Clone ID Related Accession (Same CDS sequence) XM_015475792.1 , XM_024978928.1 , XM_015467325.1 , XM_015475792.2
    Accession Version XM_015475792.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331278.1
    CDS107..2185
    Translation

    Target ORF information:

    RefSeq Version XM_015475792.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09251
    Clone ID Related Accession (Same CDS sequence) XM_015475792.1 , XM_024978928.1 , XM_015467325.1 , XM_015475792.2
    Accession Version XM_024978928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834696.1
    CDS234..2312
    Translation

    Target ORF information:

    RefSeq Version XM_024978928.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09251
    Clone ID Related Accession (Same CDS sequence) XM_015475792.1 , XM_024978928.1 , XM_015467325.1 , XM_015475792.2
    Accession Version XM_015467325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322811.1
    CDS107..2185
    Translation

    Target ORF information:

    RefSeq Version XM_015467325.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09251
    Clone ID Related Accession (Same CDS sequence) XM_015475792.1 , XM_024978928.1 , XM_015467325.1 , XM_015475792.2
    Accession Version XM_015475792.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331278.1
    CDS158..2236
    Translation

    Target ORF information:

    RefSeq Version XM_015475792.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475792.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGGTCC TGGAGCGGAC GGAGGGAAGA GAGGAGGAAA GCGGGATGAC TTTGCCTGGT 
    GGAAACGAAT GCAGAAGGGG AAGGTGCCCT GGGACGACAA GGATCTCCGC AGTCTGGCCG
    TGCTGGGGGC AGGTGTCGTT GCAGGATTTC TCTACTTCTA TTTCCGAGAT CCTGGGAAGG
    AAATCACTTG GAAGCACTTC GTGCAGTATT ACCTGGCGAG AGGTGGATCG GCTGGAAGTG
    GTGAATAAAC AGTTCGTGCG TGTTATTCCT GCCCCTGGGA CATCACTCGA GAAGTATGCG
    TGGTTCAGCA TTGGCAGTGT GGACACCTTC GAGCGCAACC TGGAGACCGC CCAATGGGAG
    CTAGGCATCG AGCCCCCCAA CCAGACGGCC GTGGTCTACA CCACCGAGAG CGATGGGACT
    TTCTTGCGAA GCCTGGTGCC CACCCTTCTC CTGATTGGCA TCTTCCTCTA TGCCATGAGG
    AGGGGTCCAA TGGGGGCTGG GCGTGGTGGG CGAGGAGGGC GCCTCTTCGG CATTGGTGAG
    ACAATAGCCA AGATCATCAA AGATGACATC GGTGTGCGGT TTGCAGACGT GGCTGGTTGC
    GAAGAAGCCA AGCTGGAGAT CATGGAGTTT GTGAATTTCC TGAAGAACCC CAAACAGTAC
    CAGGACCTTG GGGCCAAAAT TCCAAAGGGA GCCCTGCTCA CAGGTCCTCC CGGTACCGGC
    AAGACGCTTC TCGCCAAGGC AACCGCAGGG GAGGCTGGTG TGCCCTTCAT CACCGTGAAT
    GGGTCTGAGT TCCTGGAGAT GTTTGTTGGT GTTGGCCCAG CTCGGGTTCG TGACATGTTT
    GCACTGGCCC GGAAAAACGC TCCCTGTATC TTGTTTGTGG ACGAGATTGA TGCAATTGGC
    CGGAAGCGTG GCCGTGGGCA CTTTGGAGGC CAGAGTGAGC AGGAGAACAC CCTGAACCAG
    CTGCTCGTGG AGATGGACGG ATTCAACTCT ACCACGAACG TTGTCGTGCT GGCTGGAACC
    AACCGCCCTG ACGTCCTTGA CCCAGCTCTG ATGCGGCCGG GCCGCTTCGA CCGTCAGATT
    TACATTGGGC CCCCGGACAT CAAAGGCAGG TCGTCTATCT TCAGGGTCCA CCTGCGTCCA
    CTCAAGTTGG ACGAGAGCCT CAGCAAGGAT GCTCTGGCAA GGAAGCTTGC GGCCCTCACT
    CCAGGCTTCA CAGGTGCTGA TATTTCTAAT GTTTGCAATG AAGCCGCCCT GATCGCCGCT
    CGCCACCTAA ACCCCTCTGT CGGGGAGAAG CACTTCGAGC AGGCCATCGA GAGGGTCATT
    GGAGGCCTTG AGAAGAAGAC ACAGGTCCTG CAGCCTGGCG AGAAGATGAC AGTGGCCTAC
    CACGAGGCTG GCCATGCGGT GGTGGGCTGG TTCCTGGAAC ACGCAGACCC CCTGCTCAAG
    GTGTCCATCG TCCCCCGGGG CAAGGGGCTC GGCTATGCCC AGTGTCTGCC CAGGGAGCAG
    TACCTGTACA CACGGGAGCA GCTCTTTGAC CGCATGTGTG CCATGCTGGG CGGCCGCGTG
    GCTGAGCAGC TGTTCTTCGG GCGGGTCACC ACGGGGGCCC AGGATGACCT GAGGAAGGTC
    ACCCAGAGCG CCTATGCCCA GATTGTGCAG TTCGGGATGA GCGAGAAGCT GGGCCAGGTG
    TCCTTCGATC TCCCGCGGCC AGGTGAGGCG CTGGTGGAGA AACCGTTCAG CGAGGCCACG
    GCCCAGCTCA TAGACGAGGA GGTGCGGCGG CTCATTGGTT CTGCGCATGC GCGCACCCTG
    GACCTGCTCA CGCGCTGCCG CGAGCAGGTG GACAAGGTGG GCAGGCGGCT GCTGGAGAAG
    GAGGTGCTGG AGCGGGCGGA CATGGTGGAG CTGCTCGGGC CGCGGCCGTT CGCCGAGAAG
    GTCACCTACG AGGAGCTCGT GGAGGGCACC GGCGGCCTGG AGGAGGACAC GGCCCTTCCC
    GAGGGTCTGC AGGGCTGGCG TGGGGGGCCC ACTGCAGGAG AGCCCAGCCT AGCACCTCCG
    CCTGGAGAGC AGCCTCTGGG ACCCCAGGAA GCAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09252
    Clone ID Related Accession (Same CDS sequence) XM_024978929.1 , XM_005218788.1 , XM_005192682.1 , XM_005218788.2
    Accession Version XM_024978929.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834697.1
    CDS309..2324
    Translation

    Target ORF information:

    RefSeq Version XM_024978929.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09252
    Clone ID Related Accession (Same CDS sequence) XM_024978929.1 , XM_005218788.1 , XM_005192682.1 , XM_005218788.2
    Accession Version XM_005218788.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218845.1
    CDS306..2321
    Translation

    Target ORF information:

    RefSeq Version XM_005218788.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218788.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09252
    Clone ID Related Accession (Same CDS sequence) XM_024978929.1 , XM_005218788.1 , XM_005192682.1 , XM_005218788.2
    Accession Version XM_005192682.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192739.1
    CDS306..2321
    Translation

    Target ORF information:

    RefSeq Version XM_005192682.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192682.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09252
    Clone ID Related Accession (Same CDS sequence) XM_024978929.1 , XM_005218788.1 , XM_005192682.1 , XM_005218788.2
    Accession Version XM_005218788.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AFG3-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218845.1
    CDS306..2321
    Translation

    Target ORF information:

    RefSeq Version XM_005218788.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AFG3-like protein 1 (LOC532875), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218788.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCAGAAGG GGAAGGTGCC CTGGGACGAC AAGGATCTCC GCAGTCTGGC CGTGCTGGGG 
    GCAGGTGTCG TTGCAGGATT TCTCTACTTC TATTTCCGAG ATCCTGGGAA GGAAATCACT
    TGGAAGCACT TCGTGCAGTA TTACCTGGCG AGAGGTCTGG TGGATCGGCT GGAAGTGGTG
    AATAAACAGT TCGTGCGTGT TATTCCTGCC CCTGGGACAT CACTCGAGAA GTATGCGTGG
    TTCAGCATTG GCAGTGTGGA CACCTTCGAG CGCAACCTGG AGACCGCCCA ATGGGAGCTA
    GGCATCGAGC CCCCCAACCA GACGGCCGTG GTCTACACCA CCGAGAGCGA TGGGACTTTC
    TTGCGAAGCC TGGTGCCCAC CCTTCTCCTG ATTGGCATCT TCCTCTATGC CATGAGGAGG
    GGTCCAATGG GGGCTGGGCG TGGTGGGCGA GGAGGGCGCC TCTTCGGCAT TGGTGAGACA
    ATAGCCAAGA TCATCAAAGA TGACATCGGT GTGCGGTTTG CAGACGTGGC TGGTTGCGAA
    GAAGCCAAGC TGGAGATCAT GGAGTTTGTG AATTTCCTGA AGAACCCCAA ACAGTACCAG
    GACCTTGGGG CCAAAATTCC AAAGGGAGCC CTGCTCACAG GTCCTCCCGG TACCGGCAAG
    ACGCTTCTCG CCAAGGCAAC CGCAGGGGAG GCTGGTGTGC CCTTCATCAC CGTGAATGGG
    TCTGAGTTCC TGGAGATGTT TGTTGGTGTT GGCCCAGCTC GGGTTCGTGA CATGTTTGCA
    CTGGCCCGGA AAAACGCTCC CTGTATCTTG TTTGTGGACG AGATTGATGC AATTGGCCGG
    AAGCGTGGCC GTGGGCACTT TGGAGGCCAG AGTGAGCAGG AGAACACCCT GAACCAGCTG
    CTCGTGGAGA TGGACGGATT CAACTCTACC ACGAACGTTG TCGTGCTGGC TGGAACCAAC
    CGCCCTGACG TCCTTGACCC AGCTCTGATG CGGCCGGGCC GCTTCGACCG TCAGATTTAC
    ATTGGGCCCC CGGACATCAA AGGCAGGTCG TCTATCTTCA GGGTCCACCT GCGTCCACTC
    AAGTTGGACG AGAGCCTCAG CAAGGATGCT CTGGCAAGGA AGCTTGCGGC CCTCACTCCA
    GGCTTCACAG GTGCTGATAT TTCTAATGTT TGCAATGAAG CCGCCCTGAT CGCCGCTCGC
    CACCTAAACC CCTCTGTCGG GGAGAAGCAC TTCGAGCAGG CCATCGAGAG GGTCATTGGA
    GGCCTTGAGA AGAAGACACA GGTCCTGCAG CCTGGCGAGA AGATGACAGT GGCCTACCAC
    GAGGCTGGCC ATGCGGTGGT GGGCTGGTTC CTGGAACACG CAGACCCCCT GCTCAAGGTG
    TCCATCGTCC CCCGGGGCAA GGGGCTCGGC TATGCCCAGT GTCTGCCCAG GGAGCAGTAC
    CTGTACACAC GGGAGCAGCT CTTTGACCGC ATGTGTGCCA TGCTGGGCGG CCGCGTGGCT
    GAGCAGCTGT TCTTCGGGCG GGTCACCACG GGGGCCCAGG ATGACCTGAG GAAGGTCACC
    CAGAGCGCCT ATGCCCAGAT TGTGCAGTTC GGGATGAGCG AGAAGCTGGG CCAGGTGTCC
    TTCGATCTCC CGCGGCCAGG TGAGGCGCTG GTGGAGAAAC CGTTCAGCGA GGCCACGGCC
    CAGCTCATAG ACGAGGAGGT GCGGCGGCTC ATTGGTTCTG CGCATGCGCG CACCCTGGAC
    CTGCTCACGC GCTGCCGCGA GCAGGTGGAC AAGGTGGGCA GGCGGCTGCT GGAGAAGGAG
    GTGCTGGAGC GGGCGGACAT GGTGGAGCTG CTCGGGCCGC GGCCGTTCGC CGAGAAGGTC
    ACCTACGAGG AGCTCGTGGA GGGCACCGGC GGCCTGGAGG AGGACACGGC CCTTCCCGAG
    GGTCTGCAGG GCTGGCGTGG GGGGCCCACT GCAGGAGAGC CCAGCCTAGC ACCTCCGCCT
    GGAGAGCAGC CTCTGGGACC CCAGGAAGCA AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.