Afg3l1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Afg3l1
Entrez Gene ID	114896
Full Name	AFG3-like AAA ATPase 1
Synonyms	1700047G05Rik,3110061K15Rik
General protein information	Preferred Names AFG3-like AAA ATPase 1 Names AFG3-like protein 1 AFG3(ATPase family gene 3)-like 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_054070.3	NP_473411.2	AFG3-like protein 1
XM_011248276.2	XP_011246578.1	AFG3-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC532875	XP_002694913.2
Saccharomyces cerevisiae (baker's yeast)	YTA12	NP_013807.1
Magnaporthe oryzae (rice blast fungus)	MGG_07441	XP_003711337.1
Mus musculus (house mouse)	Afg3l1	NP_473411.2
Kluyveromyces lactis	KLLA0F13706g	XP_455697.1
Schizosaccharomyces pombe (fission yeast)	yta12	NP_596797.1
Neurospora crassa	NCU01479	XP_956756.1
Danio rerio (zebrafish)	si:ch1073-174d20.2	XP_005163264.1
Rattus norvegicus (Norway rat)	Afg3l1	NP_001101926.3
Eremothecium gossypii	AGOS_ABL041W	NP_982906.2
Xenopus tropicalis (tropical clawed frog)	afg3l1p	NP_001015906.1

Gene Ontology
Bibliography

Related articles in PubMed

The Mitochondrial m-AAA Protease Prevents Demyelination and Hair Greying.
Wang S, Jacquemyn J, Murru S, Martinelli P, Barth E, Langer T, Niessen CM, Rugarli EI
PLoS genetics12(12)e1006463(2016 Dec)

Autocatalytic processing of m-AAA protease subunits in mitochondria.
Koppen M, Bonn F, Ehses S, Langer T
Molecular biology of the cell20(19)4216-24(2009 Oct)

Variable and tissue-specific subunit composition of mitochondrial m-AAA protease complexes linked to hereditary spastic paraplegia.
Koppen M, Metodiev MD, Casari G, Rugarli EI, Langer T
Molecular and cellular biology27(2)758-67(2007 Jan)

A collection of cDNAs enriched in upper cortical layers of the embryonic mouse brain.
Garc?a-Frigola C, Burgaya F, Calbet M, L?pez-Dom?nech G, de Lecea L, Soriano E
Brain research. Molecular brain research122(2)133-50(2004 Mar)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

total ablation of the m-AAA protease, by deleting both Afg3l2 and its paralogue Afg3l1, triggers progressive motor dysfunction and demyelination, owing to rapid oligodendrocyte cell death.
Title: The Mitochondrial m-AAA Protease Prevents Demyelination and Hair Greying.

Data show that Afg3l1 or Afg3l2 are required for maturation of newly imported paraplegin subunits after their cleavage by MPP.
Title: Autocatalytic processing of m-AAA protease subunits in mitochondria.

Afg3l1 encodes a protein that is targeted to the mitochondria and shows high homology to human paraplegin and other members of the ATP-dependent zinc metalloprotease family.
Title: Molecular and functional analyses of the human and mouse genes encoding AFG3L1, a mitochondrial metalloprotease homologous to the human spastic paraplegia protein.

The following Afg3l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afg3l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu72402	XM_011248276.2 Latest version!	Mus musculus AFG3-like AAA ATPase 1 (Afg3l1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu23382	NM_054070.3 Latest version!	Mus musculus AFG3-like AAA ATPase 1 (Afg3l1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Afg3l1 ( XM_011248276.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246578.1 Mus musculus AFG3-like AAA ATPase 1 (Afg3l1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72402
Clone ID Related Accession (Same CDS sequence)	XM_011248276.2
Accession Version	XM_011248276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AFG3-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAAGG GAGAATTTCC TTGGGACGAC AAGGACTTCC GGAGCCTGGC TGTTTTGGGG 
GCTGGTGTGG CTGCGGGATT TTTATATTTT TATTTCCGAG ATCCCGGAAA AGAGATCACC 
TGGAAACACT TCGTGCAGTA TTACCTGGCC AGAGGTCTGG TGGACCGGCT AGAGGTTGTG 
AACAAGCAGT TTGTGCGTGT TATTCCTGTT CCTGGGACGA CATCTGAGAG GTTCGTGTGG 
TTTAACATTG GCAGTGTTGA CACCTTTGAA CGGAACCTCG AGTCTGCTCA GTGGGAGCTG 
GGCATTGAGC CCACCAACCA GGCTGCGGTG GTCTACACTA CTGAGAGTGA TGGCTCTTTT 
CTTAGAAGTC TTGTGCCCAC TCTGGTCCTG GTTAGCATCC TCCTATATGC TATGAGGAGG 
GGTCCAATGG GGACTGGTCG CGGTGGGCGA GGAGGAGGCC TCTTCAGTGT TGGTGAGACA 
ACAGCCAAGA TCTTAAAGAA CAACATCGAT GTGCGGTTTG CAGATGTGGC TGGCTGTGAA 
GAAGCCAAAC TGGAAATTAT GGAGTTTGTG AATTTCCTGA AGAACCCAAA GCAATATCAG 
GACTTAGGAG CCAAAATTCC AAAGGGAGCG ATGCTCACTG GTCCACCTGG TACTGGCAAA 
ACACTTCTTG CAAAAGCAAC TGCTGGGGAG GCCAACGTGC CCTTCATCAC CGTGAATGGG 
TCGGAATTCC TGGAAATGTT TGTTGGTGTT GGGCCAGCAC GGGTCCGTGA CATGTTTGCA 
ATGGCCCGAA AACACGCTCC TTGTATTTTA TTCATTGATG AGATTGATGC AATTGGCAGA 
AAGCGAGGCC GTGGCCACCT GGGAGGCCAG AGTGAGCAGG AAAACACTTT AAACCAGATG 
CTTGTGGAGA TGGACGGGTT CAACTCTTCC ACTAATGTGG TAGTGTTAGC CGGCACCAAT 
CGCCCTGATA TCCTTGACCC AGCCCTGACA CGGCCTGGCC GGTTTGACCG TCAGATCTAC 
ATCGGTCCCC CTGATATCAA AGGCAGGTCC TCTATTTTCA AGGTCCACTT GCGTCCACTC 
AAGCTGGACG GAAGCCTCAG CAAGGACGCT CTTTCGAGGA AGCTGGCAGC TCTTACTCCA 
GGCTTCACTG GTGCTGATAT TTCCAATGTT TGCAATGAAG CAGCACTGAT TGCTGCCCGC 
CACCTCAGCC CTTCTGTCCA GGAGCGGCAC TTTGAGCAAG CCATCGAGAG GGTCATTGGA 
GGCCTTGAGA AGAAGACCCA GGTCCTACAA CCCAGTGAAA AGACAACTGT AGCCTACCAC 
GAGGCTGGGC ATGCAGTGGT GGGCTGGTTC TTGGAGCATG CAGACCCTCT GCTGAAGGTG 
TCCATCATAC CTCGAGGCAA GGGGCTTGGC TACGCCCAGT ACCTTCCCCG CGAGCAGTTC 
CTCTACACAC GAGAGCAGCT CTTCGACCGC ATGTGTATGA TGCTGGGGGG TAGGGTAGCT 
GAGCAGCTGT TCTTTGGTCA GATCACCACC GGAGCTCAGG ACGACCTGAG GAAGGTCACC 
CAGAGTGCCT ATGCCCAGAT TGTGCAGTTT GGGATGAGTG AGAAGCTGGG CCAGGTGTCC 
TTTGACTTCC CCAGACAAGG CGAAACCATG GTGGAGAAGC CATACAGTGA GGCTACTGCC 
CAGCTCATTG ATGAAGAGGT CCGGTGCCTC GTCAGGTCTG CCTATAATCG GACCCTGGAG 
CTGCTCACAC AGTGCCGGGA GCAGGTGGAG AAGGTTGGCA GGCGTCTCCT GGAGAAAGAA 
GTGCTGGAGA AAGCCGACAT GATAGAGCTC TTGGGCCCTC GGCCCTTTGC AGAGAAGTCC 
ACCTATGAGG AATTTGTAGA GGGCACCGGC AGCCTAGAGG AGGACACATC CCTTCCTGAG 
GGGCTGAAAG ACTGGAATAA GGGGCGGGAG GAAGGAGGCA CTGAGCGGGG CTTGCAGGAG 
AGCCCTGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246578.1
CDS	97..2088
Translation	MQKGEFPWDDKDFRSLAVLGAGVAAGFLYFYFRDPGKEITWKHFVQYYLARGLVDRLEVVNKQFVRVIPVPGTTSERFVWFNIGSVDTFERNLESAQWELGIEPTNQAAVVYTTESDGSFLRSLVPTLVLVSILLYAMRRGPMGTGRGGRGGGLFSVGETTAKILKNNIDVRFADVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAMLTGPPGTGKTLLAKATAGEANVPFITVNGSEFLEMFVGVGPARVRDMFAMARKHAPCILFIDEIDAIGRKRGRGHLGGQSEQENTLNQMLVEMDGFNSSTNVVVLAGTNRPDILDPALTRPGRFDRQIYIGPPDIKGRSSIFKVHLRPLKLDGSLSKDALSRKLAALTPGFTGADISNVCNEAALIAARHLSPSVQERHFEQAIERVIGGLEKKTQVLQPSEKTTVAYHEAGHAVVGWFLEHADPLLKVSIIPRGKGLGYAQYLPREQFLYTREQLFDRMCMMLGGRVAEQLFFGQITTGAQDDLRKVTQSAYAQIVQFGMSEKLGQVSFDFPRQGETMVEKPYSEATAQLIDEEVRCLVRSAYNRTLELLTQCREQVEKVGRRLLEKEVLEKADMIELLGPRPFAEKSTYEEFVEGTGSLEEDTSLPEGLKDWNKGREEGGTERGLQESPV

Target ORF information:

RefSeq Version	XM_011248276.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus AFG3-like AAA ATPase 1 (Afg3l1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248276.2

ORF Insert Sequence:

ATGCAAAAGG GAGAATTTCC TTGGGACGAC AAGGACTTCC GGAGCCTGGC TGTTTTGGGG 
GCTGGTGTGG CTGCGGGATT TTTATATTTT TATTTCCGAG ATCCCGGAAA AGAGATCACC 
TGGAAACACT TCGTGCAGTA TTACCTGGCC AGAGGTCTGG TGGACCGGCT AGAGGTTGTG 
AACAAGCAGT TTGTGCGTGT TATTCCTGTT CCTGGGACGA CATCTGAGAG GTTCGTGTGG 
TTTAACATTG GCAGTGTTGA CACCTTTGAA CGGAACCTCG AGTCTGCTCA GTGGGAGCTG 
GGCATTGAGC CCACCAACCA GGCTGCGGTG GTCTACACTA CTGAGAGTGA TGGCTCTTTT 
CTTAGAAGTC TTGTGCCCAC TCTGGTCCTG GTTAGCATCC TCCTATATGC TATGAGGAGG 
GGTCCAATGG GGACTGGTCG CGGTGGGCGA GGAGGAGGCC TCTTCAGTGT TGGTGAGACA 
ACAGCCAAGA TCTTAAAGAA CAACATCGAT GTGCGGTTTG CAGATGTGGC TGGCTGTGAA 
GAAGCCAAAC TGGAAATTAT GGAGTTTGTG AATTTCCTGA AGAACCCAAA GCAATATCAG 
GACTTAGGAG CCAAAATTCC AAAGGGAGCG ATGCTCACTG GTCCACCTGG TACTGGCAAA 
ACACTTCTTG CAAAAGCAAC TGCTGGGGAG GCCAACGTGC CCTTCATCAC CGTGAATGGG 
TCGGAATTCC TGGAAATGTT TGTTGGTGTT GGGCCAGCAC GGGTCCGTGA CATGTTTGCA 
ATGGCCCGAA AACACGCTCC TTGTATTTTA TTCATTGATG AGATTGATGC AATTGGCAGA 
AAGCGAGGCC GTGGCCACCT GGGAGGCCAG AGTGAGCAGG AAAACACTTT AAACCAGATG 
CTTGTGGAGA TGGACGGGTT CAACTCTTCC ACTAATGTGG TAGTGTTAGC CGGCACCAAT 
CGCCCTGATA TCCTTGACCC AGCCCTGACA CGGCCTGGCC GGTTTGACCG TCAGATCTAC 
ATCGGTCCCC CTGATATCAA AGGCAGGTCC TCTATTTTCA AGGTCCACTT GCGTCCACTC 
AAGCTGGACG GAAGCCTCAG CAAGGACGCT CTTTCGAGGA AGCTGGCAGC TCTTACTCCA 
GGCTTCACTG GTGCTGATAT TTCCAATGTT TGCAATGAAG CAGCACTGAT TGCTGCCCGC 
CACCTCAGCC CTTCTGTCCA GGAGCGGCAC TTTGAGCAAG CCATCGAGAG GGTCATTGGA 
GGCCTTGAGA AGAAGACCCA GGTCCTACAA CCCAGTGAAA AGACAACTGT AGCCTACCAC 
GAGGCTGGGC ATGCAGTGGT GGGCTGGTTC TTGGAGCATG CAGACCCTCT GCTGAAGGTG 
TCCATCATAC CTCGAGGCAA GGGGCTTGGC TACGCCCAGT ACCTTCCCCG CGAGCAGTTC 
CTCTACACAC GAGAGCAGCT CTTCGACCGC ATGTGTATGA TGCTGGGGGG TAGGGTAGCT 
GAGCAGCTGT TCTTTGGTCA GATCACCACC GGAGCTCAGG ACGACCTGAG GAAGGTCACC 
CAGAGTGCCT ATGCCCAGAT TGTGCAGTTT GGGATGAGTG AGAAGCTGGG CCAGGTGTCC 
TTTGACTTCC CCAGACAAGG CGAAACCATG GTGGAGAAGC CATACAGTGA GGCTACTGCC 
CAGCTCATTG ATGAAGAGGT CCGGTGCCTC GTCAGGTCTG CCTATAATCG GACCCTGGAG 
CTGCTCACAC AGTGCCGGGA GCAGGTGGAG AAGGTTGGCA GGCGTCTCCT GGAGAAAGAA 
GTGCTGGAGA AAGCCGACAT GATAGAGCTC TTGGGCCCTC GGCCCTTTGC AGAGAAGTCC 
ACCTATGAGG AATTTGTAGA GGGCACCGGC AGCCTAGAGG AGGACACATC CCTTCCTGAG 
GGGCTGAAAG ACTGGAATAA GGGGCGGGAG GAAGGAGGCA CTGAGCGGGG CTTGCAGGAG 
AGCCCTGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afg3l1 ( NM_054070.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_473411.2 Mus musculus AFG3-like AAA ATPase 1 (Afg3l1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23382
Clone ID Related Accession (Same CDS sequence)	NM_054070.3
Accession Version	NM_054070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	AFG3-like protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158362.5, AK159647.1 and BC056978.1. On Jul 31, 2009 this sequence version replaced NM_054070.2. Transcript Variant: This variant (1) represents the longer transcript. ##Evidence-Data-START## Transcript exon combination :: BC056978.1, AK159647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGTTACTGC GGCTGGTGGG GGCGGCGGGC AGTCGAGCCC TGGCCTGGCC TTTCTCCAAG 
CTGTGGCGAT GTGGCGGATG CGCAGGGAGC GGCGGGACGG TCTGGAGCAG CGTGAGGGCC 
TGTGGCATTG CTCTGCAGGG TCATCTGGGG AGATGCTCGC AGCAGCTGGC TCTGCAGGGA 
AAACTGACTT CATTTTCCCC GAGGCTGTAT TCAAAACCTC CCAGAGGGTT TGAGAAGTTT 
TTTAAGAATA AGAAGAACAG AAAAAGTGCA AGCCCAGGAA ATTCAGTACC TCCAAAAAAA 
GAACCAAAAA ATGCTGGCCC TGGAGGAGAT GGAGGCAACA GAGGAGGGAA AGGAGATGAT 
TTTCCCTGGT GGAAACGGAT GCAAAAGGGA GAATTTCCTT GGGACGACAA GGACTTCCGG 
AGCCTGGCTG TTTTGGGGGC TGGTGTGGCT GCGGGATTTT TATATTTTTA TTTCCGAGAT 
CCCGGAAAAG AGATCACCTG GAAACACTTC GTGCAGTATT ACCTGGCCAG AGGTCTGGTG 
GACCGGCTAG AGGTTGTGAA CAAGCAGTTT GTGCGTGTTA TTCCTGTTCC TGGGACGACA 
TCTGAGAGGT TCGTGTGGTT TAACATTGGC AGTGTTGACA CCTTTGAACG GAACCTCGAG 
TCTGCTCAGT GGGAGCTGGG CATTGAGCCC ACCAACCAGG CTGCGGTGGT CTACACTACT 
GAGAGTGATG GCTCTTTTCT TAGAAGTCTT GTGCCCACTC TGGTCCTGGT TAGCATCCTC 
CTATATGCTA TGAGGAGGGG TCCAATGGGG ACTGGTCGCG GTGGGCGAGG AGGAGGCCTC 
TTCAGTGTTG GTGAGACAAC AGCCAAGATC TTAAAGAACA ACATCGATGT GCGGTTTGCA 
GATGTGGCTG GCTGTGAAGA AGCCAAACTG GAAATTATGG AGTTTGTGAA TTTCCTGAAG 
AACCCAAAGC AATATCAGGA CTTAGGAGCC AAAATTCCAA AGGGAGCGAT GCTCACTGGT 
CCACCTGGTA CTGGCAAAAC ACTTCTTGCA AAAGCAACTG CTGGGGAGGC CAACGTGCCC 
TTCATCACCG TGAATGGGTC GGAATTCCTG GAAATGTTTG TTGGTGTTGG GCCAGCACGG 
GTCCGTGACA TGTTTGCAAT GGCCCGAAAA CACGCTCCTT GTATTTTATT CATTGATGAG 
ATTGATGCAA TTGGCAGAAA GCGAGGCCGT GGCCACCTGG GAGGCCAGAG TGAGCAGGAA 
AACACTTTAA ACCAGATGCT TGTGGAGATG GACGGGTTCA ACTCTTCCAC TAATGTGGTA 
GTGTTAGCCG GCACCAATCG CCCTGATATC CTTGACCCAG CCCTGACACG GCCTGGCCGG 
TTTGACCGTC AGATCTACAT CGGTCCCCCT GATATCAAAG GCAGGTCCTC TATTTTCAAG 
GTCCACTTGC GTCCACTCAA GCTGGACGGA AGCCTCAGCA AGGACGCTCT TTCGAGGAAG 
CTGGCAGCTC TTACTCCAGG CTTCACTGGT GCTGATATTT CCAATGTTTG CAATGAAGCA 
GCACTGATTG CTGCCCGCCA CCTCAGCCCT TCTGTCCAGG AGCGGCACTT TGAGCAAGCC 
ATCGAGAGGG TCATTGGAGG CCTTGAGAAG AAGACCCAGG TCCTACAACC CAGTGAAAAG 
ACAACTGTAG CCTACCACGA GGCTGGGCAT GCAGTGGTGG GCTGGTTCTT GGAGCATGCA 
GACCCTCTGC TGAAGGTGTC CATCATACCT CGAGGCAAGG GGCTTGGCTA CGCCCAGTAC 
CTTCCCCGCG AGCAGTTCCT CTACACACGA GAGCAGCTCT TCGACCGCAT GTGTATGATG 
CTGGGGGGTA GGGTAGCTGA GCAGCTGTTC TTTGGTCAGA TCACCACCGG AGCTCAGGAC 
GACCTGAGGA AGGTCACCCA GAGTGCCTAT GCCCAGATTG TGCAGTTTGG GATGAGTGAG 
AAGCTGGGCC AGGTGTCCTT TGACTTCCCC AGACAAGGCG AAACCATGGT GGAGAAGCCA 
TACAGTGAGG CTACTGCCCA GCTCATTGAT GAAGAGGTCC GGTGCCTCGT CAGGTCTGCC 
TATAATCGGA CCCTGGAGCT GCTCACACAG TGCCGGGAGC AGGTGGAGAA GGTTGGCAGG 
CGTCTCCTGG AGAAAGAAGT GCTGGAGAAA GCCGACATGA TAGAGCTCTT GGGCCCTCGG 
CCCTTTGCAG AGAAGTCCAC CTATGAGGAA TTTGTAGAGG GCACCGGCAG CCTAGAGGAG 
GACACATCCC TTCCTGAGGG GCTGAAAGAC TGGAATAAGG GGCGGGAGGA AGGAGGCACT 
GAGCGGGGCT TGCAGGAGAG CCCTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473411.2
CDS	49..2418
Translation	MLLRLVGAAGSRALAWPFSKLWRCGGCAGSGGTVWSSVRACGIALQGHLGRCSQQLALQGKLTSFSPRLYSKPPRGFEKFFKNKKNRKSASPGNSVPPKKEPKNAGPGGDGGNRGGKGDDFPWWKRMQKGEFPWDDKDFRSLAVLGAGVAAGFLYFYFRDPGKEITWKHFVQYYLARGLVDRLEVVNKQFVRVIPVPGTTSERFVWFNIGSVDTFERNLESAQWELGIEPTNQAAVVYTTESDGSFLRSLVPTLVLVSILLYAMRRGPMGTGRGGRGGGLFSVGETTAKILKNNIDVRFADVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAMLTGPPGTGKTLLAKATAGEANVPFITVNGSEFLEMFVGVGPARVRDMFAMARKHAPCILFIDEIDAIGRKRGRGHLGGQSEQENTLNQMLVEMDGFNSSTNVVVLAGTNRPDILDPALTRPGRFDRQIYIGPPDIKGRSSIFKVHLRPLKLDGSLSKDALSRKLAALTPGFTGADISNVCNEAALIAARHLSPSVQERHFEQAIERVIGGLEKKTQVLQPSEKTTVAYHEAGHAVVGWFLEHADPLLKVSIIPRGKGLGYAQYLPREQFLYTREQLFDRMCMMLGGRVAEQLFFGQITTGAQDDLRKVTQSAYAQIVQFGMSEKLGQVSFDFPRQGETMVEKPYSEATAQLIDEEVRCLVRSAYNRTLELLTQCREQVEKVGRRLLEKEVLEKADMIELLGPRPFAEKSTYEEFVEGTGSLEEDTSLPEGLKDWNKGREEGGTERGLQESPV

Target ORF information:

RefSeq Version	NM_054070.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus AFG3-like AAA ATPase 1 (Afg3l1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054070.3

ORF Insert Sequence:

ATGTTACTGC GGCTGGTGGG GGCGGCGGGC AGTCGAGCCC TGGCCTGGCC TTTCTCCAAG 
CTGTGGCGAT GTGGCGGATG CGCAGGGAGC GGCGGGACGG TCTGGAGCAG CGTGAGGGCC 
TGTGGCATTG CTCTGCAGGG TCATCTGGGG AGATGCTCGC AGCAGCTGGC TCTGCAGGGA 
AAACTGACTT CATTTTCCCC GAGGCTGTAT TCAAAACCTC CCAGAGGGTT TGAGAAGTTT 
TTTAAGAATA AGAAGAACAG AAAAAGTGCA AGCCCAGGAA ATTCAGTACC TCCAAAAAAA 
GAACCAAAAA ATGCTGGCCC TGGAGGAGAT GGAGGCAACA GAGGAGGGAA AGGAGATGAT 
TTTCCCTGGT GGAAACGGAT GCAAAAGGGA GAATTTCCTT GGGACGACAA GGACTTCCGG 
AGCCTGGCTG TTTTGGGGGC TGGTGTGGCT GCGGGATTTT TATATTTTTA TTTCCGAGAT 
CCCGGAAAAG AGATCACCTG GAAACACTTC GTGCAGTATT ACCTGGCCAG AGGTCTGGTG 
GACCGGCTAG AGGTTGTGAA CAAGCAGTTT GTGCGTGTTA TTCCTGTTCC TGGGACGACA 
TCTGAGAGGT TCGTGTGGTT TAACATTGGC AGTGTTGACA CCTTTGAACG GAACCTCGAG 
TCTGCTCAGT GGGAGCTGGG CATTGAGCCC ACCAACCAGG CTGCGGTGGT CTACACTACT 
GAGAGTGATG GCTCTTTTCT TAGAAGTCTT GTGCCCACTC TGGTCCTGGT TAGCATCCTC 
CTATATGCTA TGAGGAGGGG TCCAATGGGG ACTGGTCGCG GTGGGCGAGG AGGAGGCCTC 
TTCAGTGTTG GTGAGACAAC AGCCAAGATC TTAAAGAACA ACATCGATGT GCGGTTTGCA 
GATGTGGCTG GCTGTGAAGA AGCCAAACTG GAAATTATGG AGTTTGTGAA TTTCCTGAAG 
AACCCAAAGC AATATCAGGA CTTAGGAGCC AAAATTCCAA AGGGAGCGAT GCTCACTGGT 
CCACCTGGTA CTGGCAAAAC ACTTCTTGCA AAAGCAACTG CTGGGGAGGC CAACGTGCCC 
TTCATCACCG TGAATGGGTC GGAATTCCTG GAAATGTTTG TTGGTGTTGG GCCAGCACGG 
GTCCGTGACA TGTTTGCAAT GGCCCGAAAA CACGCTCCTT GTATTTTATT CATTGATGAG 
ATTGATGCAA TTGGCAGAAA GCGAGGCCGT GGCCACCTGG GAGGCCAGAG TGAGCAGGAA 
AACACTTTAA ACCAGATGCT TGTGGAGATG GACGGGTTCA ACTCTTCCAC TAATGTGGTA 
GTGTTAGCCG GCACCAATCG CCCTGATATC CTTGACCCAG CCCTGACACG GCCTGGCCGG 
TTTGACCGTC AGATCTACAT CGGTCCCCCT GATATCAAAG GCAGGTCCTC TATTTTCAAG 
GTCCACTTGC GTCCACTCAA GCTGGACGGA AGCCTCAGCA AGGACGCTCT TTCGAGGAAG 
CTGGCAGCTC TTACTCCAGG CTTCACTGGT GCTGATATTT CCAATGTTTG CAATGAAGCA 
GCACTGATTG CTGCCCGCCA CCTCAGCCCT TCTGTCCAGG AGCGGCACTT TGAGCAAGCC 
ATCGAGAGGG TCATTGGAGG CCTTGAGAAG AAGACCCAGG TCCTACAACC CAGTGAAAAG 
ACAACTGTAG CCTACCACGA GGCTGGGCAT GCAGTGGTGG GCTGGTTCTT GGAGCATGCA 
GACCCTCTGC TGAAGGTGTC CATCATACCT CGAGGCAAGG GGCTTGGCTA CGCCCAGTAC 
CTTCCCCGCG AGCAGTTCCT CTACACACGA GAGCAGCTCT TCGACCGCAT GTGTATGATG 
CTGGGGGGTA GGGTAGCTGA GCAGCTGTTC TTTGGTCAGA TCACCACCGG AGCTCAGGAC 
GACCTGAGGA AGGTCACCCA GAGTGCCTAT GCCCAGATTG TGCAGTTTGG GATGAGTGAG 
AAGCTGGGCC AGGTGTCCTT TGACTTCCCC AGACAAGGCG AAACCATGGT GGAGAAGCCA 
TACAGTGAGG CTACTGCCCA GCTCATTGAT GAAGAGGTCC GGTGCCTCGT CAGGTCTGCC 
TATAATCGGA CCCTGGAGCT GCTCACACAG TGCCGGGAGC AGGTGGAGAA GGTTGGCAGG 
CGTCTCCTGG AGAAAGAAGT GCTGGAGAAA GCCGACATGA TAGAGCTCTT GGGCCCTCGG 
CCCTTTGCAG AGAAGTCCAC CTATGAGGAA TTTGTAGAGG GCACCGGCAG CCTAGAGGAG 
GACACATCCC TTCCTGAGGG GCTGAAAGAC TGGAATAAGG GGCGGGAGGA AGGAGGCACT 
GAGCGGGGCT TGCAGGAGAG CCCTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Mitochondrial m-AAA Protease Prevents Demyelination and Hair Greying.
PLoS genetics12(12)e1006463(2016 Dec)
Wang S,Jacquemyn J,Murru S,Martinelli P,Barth E,Langer T,Niessen CM,Rugarli EI

Autocatalytic processing of m-AAA protease subunits in mitochondria.
Molecular biology of the cell20(19)4216-24(2009 Oct)
Koppen M,Bonn F,Ehses S,Langer T

Variable and tissue-specific subunit composition of mitochondrial m-AAA protease complexes linked to hereditary spastic paraplegia.
Molecular and cellular biology27(2)758-67(2007 Jan)
Koppen M,Metodiev MD,Casari G,Rugarli EI,Langer T

A collection of cDNAs enriched in upper cortical layers of the embryonic mouse brain.
Brain research. Molecular brain research122(2)133-50(2004 Mar)
Garc?a-Frigola C,Burgaya F,Calbet M,L?pez-Dom?nech G,de Lecea L,Soriano E

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Afg3l1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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