TCB3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TCB3
Entrez Gene ID	854903
Full Name	Tcb3p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182431.1	NP_013639.1	Tcb3p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TCB3	NP_013639.1
Eremothecium gossypii	AGOS_AER148W	NP_985007.1
Arabidopsis thaliana (thale cress)	SYTC	NP_974729.1
Magnaporthe oryzae (rice blast fungus)	MGG_09947	XP_003710051.1
Neurospora crassa	NCU03263	XP_964416.2
Kluyveromyces lactis	KLLA0B04708g	XP_451743.1
Schizosaccharomyces pombe (fission yeast)	syn1	NP_593974.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The tricalbins function as multimers in membrane-trafficking events. tcb1, tcb3 double mutants have an altered vacuole morphology and are hypersensitive to cyclohexamide. RSP5 was identified as a single-copy suppressor of the cyclohexamide sensitivity.
Title: Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking.

The C-terminal C2 domains of tricalbins 1 and 3 are calcium-dependent lipid-binding units comparable to mammalian synaptotagmin C2 domains.
Title: The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family.

The following TCB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03906	NM_001182431.1 Latest version!	Saccharomyces cerevisiae S288C Tcb3p (TCB3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCB3 ( NM_001182431.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013639.1 Saccharomyces cerevisiae S288C Tcb3p (TCB3), partial mRNA.

Price & Availability↑

CloneID	OSi03906
Clone ID Related Accession (Same CDS sequence)	NM_001182431.1
Accession Version	NM_001182431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Tcb3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTGGCA TCAAAGCTCA AGTCCATCCC CCACCTGATA GTACCCTATT TCATGAGGAG 
GAGAAGAAGA AAGTAGGAGG CAATTTACCT CAAAAGGTCA TAAATCAACA AGAAAGGGGT 
TCTGATCACG CTCCATCGGG TCACCATCAA TACCACCAAC TGATTAACCA TGACGCAAAT 
GACACAAAGA CCTCAAATTC AGTTTCTGAT GTGTCTAAAG GTCAGAAAAC TGCTGACTCC 
AACCCGGAAG GTAAGAAACA GTCATCAAAA GACATATTTG TTGCCTCCAG CGCTCAAAAA 
ACCAATCAAT TGCCCGGTCC CAACCCACAG GGAAGCATAG GAGCCGTGCC ATTGGAAGGT 
TTACGTCCGA AGGAATTCAG ATCAGCACCA TCTAGGAAGC CAAATAAATT CGACACTTCG 
ATTACTAAGC CTGGCGTCTT AGACGACTTA GGCAAACTTG ATGAAAAGGA TATTAAGGAA 
AAATTTCACC TAGATTCCGA CGACAAGTTA TTTCCATGGC AAAATGTTGG TGAGTTCCAT 
GCTTCAGGAA AGGGTTCGCC AAATACAAAG ATGTCCAGGG TTATAAAAGC TTACATTCTG 
GAAAATTTTT ATAACGATTG GTACTGTAAT ATAGCCACCG TTCTTGGAAC TTGTTTCTTC 
TCATGGTTAT TTGCTTACAT TGGGTTTTCA TGGTGGTCTA TGATATTTAT CTTCTTGGGA 
ACTGCGACCG TTTACAACGC AGAATATACA AGATTCAACA GAAATATCAG AGATGACTTG 
AAAAGAGTTA CAGTCGAAGA AACCTTGTCG GATCGCGTTG AATCCACTAC GTGGTTGAAT 
TCATTTTTAT CAAAATTTTG GGTGATTTAC ATGCCAGTTT TATCTCAACA AGTCAAAGAT 
AACGTTAACC CTCAACTGGC AGGTGTTGCT CCAGGTTACG GTATCGATGC GTTAGCTATC 
GATGAATTCA CTCTGGGCTC TAAAGCTCCC ACCATAAAAG GTATTAAATC GTACACCAAG 
ACTGGTAAAA ACACTGTTGA AATGGATTGG TCATTTGCAT TCACCCCAAG CGATGTCTCG 
GATATGACAG CTACTGAAGC TAGGGAGAAG ATCAATCCAA AAATATCTCT GGGTGTCACG 
TTAGGAAAAA GTTTTGTCTC TAAAACAATG CCTATTTTGG TTGAAGACAT TAACGTTGCT 
GGTAAAATGC GTATTAAAGT TGAATTTGGT AAAGCTTTCC CAAATATCAA AATTGTTTCT 
TTACAACTTT TAGAACCACC TTTGATTGAT TTCGCACTGA AACCAATTGG TGGTGATACT 
TTAGGTCTTG ATGTTATGTC ATTCTTGCCT GGTTTGAAGA GTTTTGTTAA AAACATTATC 
AACTCCAATA TAGGGCCTAT GCTATTCCCT CCGAACCATT TGGATATTAA TGTTGAAGAC 
ATTATGGCTG CTCAATCAAA AGAAGCTATT GGTGTCCTTG CCGTAACCAT TGCTTCTGCC 
GACTCTTTGA AAGGCTCAGA TTTCATTACC AATACTGTCG ATCCTTATAT TGTTATGACT 
ACCGAAGATG CTGTGCCTGG TACAGATGAA GAAGTGCGTA CATCTATCAA ATCAAATGTT 
AAAAATCCAC GTTGGAACGA AACCAAATAT CTATTATTAA ACACCTTAGA GCAAAAGTTA 
AACTTAAAGT GCTTTGACTT CAATGATGTA AGAAAAGATA CCGTAATTGG TGATCTTCAA 
CTTGACTTGG CAGATTTACT ACAAAACCCT GTTTTGGATA ATCAAACTGC TGAATTAAGA 
TCCGGTACAA AATCAAAAGG TATTTTACAT TATTCCTTAC ACTGGTTCCC TGTGAAAGAA 
GATAAATCAG AGGAAAAAGC AGTTGAGCGT GCCGAAGCTA AGGCCAAGGG CAAGAAAGAA 
GATGAAAACG AGGACACTAC TGAAAAAGAA GAAGACGAGA ATGAAGAAAG TTCTCAAACT 
GATGTCGGGA TTGCCAAGAT CACTTTACAA AAGGTCAAAT ATCTGGATAC AACCAGTTCT 
ATGACCGGTA GCTTGAGCCC ATGTGCTGAA TTATTCATTG ATGGACAAAA AGTAAAGAGC 
TATAGAACTT TGAGACGTAT CAATGAGCCA TCTTGGAATG AGACCATCGA AGTTTTGGTT 
CCATCAAAAT CTAACTCTAA GTTTGTCCTA AAAATATTCG ATGACAGAAT GAATGGTAAG 
GCGCTGATCT GTGAGTATTC ATCTTCTTTA GATGATATAA TGACTACTTT AGACACTGCT 
CAAGAGTTTG TTAAAGGCTC ACCACAAGGT GACATTTATT TGGATGTTTC TTGGAAATCA 
ATTGAAATGA CCGGAGCTTT TGCCGCTGCA AACTCTGTAA GCGAACCTAT TGGTTGTATT 
AAGCTAGACG TTAAGGATGC CATTATCAAG GGTGACTTAT CCGGTGTAGG GGATGTTGAT 
CCATATTACA CCGTATCGTT GAATAGACGT GTTCTTTACA AGTCCATATA TCATTCTGAT 
ACGGATCATC CCATTTTTGA CAACAGCACC TACGTTCCTA TCTTCTCTCC AAATCAAATT 
TTGACTCTCG AATTTCATGA TTATCAAAAG ATCGGCAAAG ACCGTTTCAT TGGCTCTGTA 
CAAATTCCTA CATCAAATGT TTTCAAAAAA GATCCTAAAT CAGGAAAATA TGTTGGGAAT 
AATGGCAAAG AAGAAATTTC AAAACTAAAA TTAAAAGACC ACGAACACAA AGTTACCGAA 
AGCATTGTCA ATGTTTCAAC AACATTTATC CCAATCAATC TGGTGTATTC CCCTGAGGAG 
TTGGTGAATG TTGAGAAACT AGAAAAGGAG TTGAAGGAAA AGAAGAAAAA ATTCGAAGCT 
ACCCAAGAAG AAAACGAGCA AGAGATGGAA AAAAATCCAA AGGAATGGGA AGTTGCCGAG 
ATCGAAGACC CATTTGACAG CGATGAAAAA AAAATAAACA GGAAGGCCAA GTTATCTTTA 
AACGAGTTGA TCAAGCAAAA ATCTGGTATT TTGTCTATGC AAATATTGGA AGGGACTTTG 
AGCCCATCCT CTGCTTACCT AGAAATCTTA GCGGATGACA TTTCGTACCC TGTATTCATT 
TGCATGAAAC CATCTCAAGG TAAACTAAAC TCGGAGATGG CAAATATTTT CATTAGAGAT 
TTGAATTACA GTAAACTACA TTTTAGAGTA TCGAAGAAAC ATATTGCCAA AGATTCAGAT 
GATGTCATAT CCGAAACTTC CTATAGTACA TTGAAGCTAC TAAAGCAAGC TTACGAAGAG 
CCCATGTGGT TAAACTTCAA TGGGTCTAAA ATGAAGGTAA GATTTTTGTA CACGCCCACT 
AGCGTGAAAC TGCCTAGCAG TGAAAGTGTT GAAGACACTG GTTATTTGAA TATAAAGCTT 
ATTTCCGGAC ACGGTCTGAA GTCCGCAGAT AGGAATGGCT ATTCAGATCC ATTTGTTCAC 
ATCTTTGTCA ATGATAAAAA AGTTTTCAAA TCGAACATTA AAAAGAAAAC ATTGGATCCC 
GTATGGAACG AAGATGCTAA AATACCAATC CTTTCAAGAA GTAAGAATCA AGTCATATTT 
AATGTTCTTG ATTGGGATCG TGCAGGTGAT AATGACGACT TAGGCCAAGC TTCACTTGAC 
GTTTCCTCAT TAGAAGTTGG TAAAACTTAC AACTGGAATT TGAATTTAAA CACACAAGGA 
AGTATCAAAT TACAAGGTTC ATTCAACCCA GAATATATCA AGCCAAGTTT TGATATCGTG 
AAAGGCGGTA TCACTGATAA GCCGATGAAA ATAGCCAGTG GTGCAGCCCA TGCAACTGTT 
GGCATAGCTG GTACTGGTAT AGGAGCAGCA ACAGGAGTTG CCACTGGTGG TTTAAAGAAA 
GGTGGTCACC TTCTAAAATC TCTAGGTGGC AATCCAATGA AAAGAAGCAA GAGCAGCAAT 
GGAAATGAGT CCAACGGTGC AAAAAAATCA TCAGAGAAAA AATCTTTTGA TAGGAGATCC 
CCAAGTAATT TGAATAGCAC TAGTGTAACA CCAAGAGCTT CACTAGACTA TGATCCATCA 
GTACCTAACA CAAGTTACGC GCCCGTTCAA AGCGCATCTC CTGTAGTCAA GCCAACTGAC 
AACACTTCTA GCTCAAGCAA CAAAAAAGAT ACCCCTAGTA GCAACTCTAG AGGACATTCT 
CGTGCAAGCA GTTTTGCGCG TACTTTAGCT CCTCATGGCA CTTACAATGG TTTTATTACC 
GTGGTTGCTG CGGAAAACGT TGCCAAGCAT GTTCAAATTA AGATCTCTTT AACTCAAGGT 
GGTAGACTAA AACACATATA CAAAACGAAA AGCCAAAAAG CCAATAATGA TGGTGTTGCC 
GTATTTGATG AAGAGTGCTC GTTCAAGGCT TCTCCCGAAG CCAATTTGGT ACTGGGTGCA 
ATTTCCCATC AAAGACTATC GAGGGACAAA GATCTTGGTA TTGCTCAAAT CAACTTGGGT 
GACCCTCAAA TTCAACAAGA TGGCCAAATT TCTGTAAAAT TAGGAGACGG TCATCTGATT 
GTAAAGATTA ATTACGGTAA AGACAAGAAT GGTCAGGTAC CTCCCGTGCC AGAAGTTCCT 
CAAGAATACA CGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013639.1
CDS	1..4638
Translation	MTGIKAQVHPPPDSTLFHEEEKKKVGGNLPQKVINQQERGSDHAPSGHHQYHQLINHDANDTKTSNSVSDVSKGQKTADSNPEGKKQSSKDIFVASSAQKTNQLPGPNPQGSIGAVPLEGLRPKEFRSAPSRKPNKFDTSITKPGVLDDLGKLDEKDIKEKFHLDSDDKLFPWQNVGEFHASGKGSPNTKMSRVIKAYILENFYNDWYCNIATVLGTCFFSWLFAYIGFSWWSMIFIFLGTATVYNAEYTRFNRNIRDDLKRVTVEETLSDRVESTTWLNSFLSKFWVIYMPVLSQQVKDNVNPQLAGVAPGYGIDALAIDEFTLGSKAPTIKGIKSYTKTGKNTVEMDWSFAFTPSDVSDMTATEAREKINPKISLGVTLGKSFVSKTMPILVEDINVAGKMRIKVEFGKAFPNIKIVSLQLLEPPLIDFALKPIGGDTLGLDVMSFLPGLKSFVKNIINSNIGPMLFPPNHLDINVEDIMAAQSKEAIGVLAVTIASADSLKGSDFITNTVDPYIVMTTEDAVPGTDEEVRTSIKSNVKNPRWNETKYLLLNTLEQKLNLKCFDFNDVRKDTVIGDLQLDLADLLQNPVLDNQTAELRSGTKSKGILHYSLHWFPVKEDKSEEKAVERAEAKAKGKKEDENEDTTEKEEDENEESSQTDVGIAKITLQKVKYLDTTSSMTGSLSPCAELFIDGQKVKSYRTLRRINEPSWNETIEVLVPSKSNSKFVLKIFDDRMNGKALICEYSSSLDDIMTTLDTAQEFVKGSPQGDIYLDVSWKSIEMTGAFAAANSVSEPIGCIKLDVKDAIIKGDLSGVGDVDPYYTVSLNRRVLYKSIYHSDTDHPIFDNSTYVPIFSPNQILTLEFHDYQKIGKDRFIGSVQIPTSNVFKKDPKSGKYVGNNGKEEISKLKLKDHEHKVTESIVNVSTTFIPINLVYSPEELVNVEKLEKELKEKKKKFEATQEENEQEMEKNPKEWEVAEIEDPFDSDEKKINRKAKLSLNELIKQKSGILSMQILEGTLSPSSAYLEILADDISYPVFICMKPSQGKLNSEMANIFIRDLNYSKLHFRVSKKHIAKDSDDVISETSYSTLKLLKQAYEEPMWLNFNGSKMKVRFLYTPTSVKLPSSESVEDTGYLNIKLISGHGLKSADRNGYSDPFVHIFVNDKKVFKSNIKKKTLDPVWNEDAKIPILSRSKNQVIFNVLDWDRAGDNDDLGQASLDVSSLEVGKTYNWNLNLNTQGSIKLQGSFNPEYIKPSFDIVKGGITDKPMKIASGAAHATVGIAGTGIGAATGVATGGLKKGGHLLKSLGGNPMKRSKSSNGNESNGAKKSSEKKSFDRRSPSNLNSTSVTPRASLDYDPSVPNTSYAPVQSASPVVKPTDNTSSSSNKKDTPSSNSRGHSRASSFARTLAPHGTYNGFITVVAAENVAKHVQIKISLTQGGRLKHIYKTKSQKANNDGVAVFDEECSFKASPEANLVLGAISHQRLSRDKDLGIAQINLGDPQIQQDGQISVKLGDGHLIVKINYGKDKNGQVPPVPEVPQEYTQ

Target ORF information:

RefSeq Version	NM_001182431.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Tcb3p (TCB3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182431.1

ORF Insert Sequence:

ATGACTGGCA TCAAAGCTCA AGTCCATCCC CCACCTGATA GTACCCTATT TCATGAGGAG 
GAGAAGAAGA AAGTAGGAGG CAATTTACCT CAAAAGGTCA TAAATCAACA AGAAAGGGGT 
TCTGATCACG CTCCATCGGG TCACCATCAA TACCACCAAC TGATTAACCA TGACGCAAAT 
GACACAAAGA CCTCAAATTC AGTTTCTGAT GTGTCTAAAG GTCAGAAAAC TGCTGACTCC 
AACCCGGAAG GTAAGAAACA GTCATCAAAA GACATATTTG TTGCCTCCAG CGCTCAAAAA 
ACCAATCAAT TGCCCGGTCC CAACCCACAG GGAAGCATAG GAGCCGTGCC ATTGGAAGGT 
TTACGTCCGA AGGAATTCAG ATCAGCACCA TCTAGGAAGC CAAATAAATT CGACACTTCG 
ATTACTAAGC CTGGCGTCTT AGACGACTTA GGCAAACTTG ATGAAAAGGA TATTAAGGAA 
AAATTTCACC TAGATTCCGA CGACAAGTTA TTTCCATGGC AAAATGTTGG TGAGTTCCAT 
GCTTCAGGAA AGGGTTCGCC AAATACAAAG ATGTCCAGGG TTATAAAAGC TTACATTCTG 
GAAAATTTTT ATAACGATTG GTACTGTAAT ATAGCCACCG TTCTTGGAAC TTGTTTCTTC 
TCATGGTTAT TTGCTTACAT TGGGTTTTCA TGGTGGTCTA TGATATTTAT CTTCTTGGGA 
ACTGCGACCG TTTACAACGC AGAATATACA AGATTCAACA GAAATATCAG AGATGACTTG 
AAAAGAGTTA CAGTCGAAGA AACCTTGTCG GATCGCGTTG AATCCACTAC GTGGTTGAAT 
TCATTTTTAT CAAAATTTTG GGTGATTTAC ATGCCAGTTT TATCTCAACA AGTCAAAGAT 
AACGTTAACC CTCAACTGGC AGGTGTTGCT CCAGGTTACG GTATCGATGC GTTAGCTATC 
GATGAATTCA CTCTGGGCTC TAAAGCTCCC ACCATAAAAG GTATTAAATC GTACACCAAG 
ACTGGTAAAA ACACTGTTGA AATGGATTGG TCATTTGCAT TCACCCCAAG CGATGTCTCG 
GATATGACAG CTACTGAAGC TAGGGAGAAG ATCAATCCAA AAATATCTCT GGGTGTCACG 
TTAGGAAAAA GTTTTGTCTC TAAAACAATG CCTATTTTGG TTGAAGACAT TAACGTTGCT 
GGTAAAATGC GTATTAAAGT TGAATTTGGT AAAGCTTTCC CAAATATCAA AATTGTTTCT 
TTACAACTTT TAGAACCACC TTTGATTGAT TTCGCACTGA AACCAATTGG TGGTGATACT 
TTAGGTCTTG ATGTTATGTC ATTCTTGCCT GGTTTGAAGA GTTTTGTTAA AAACATTATC 
AACTCCAATA TAGGGCCTAT GCTATTCCCT CCGAACCATT TGGATATTAA TGTTGAAGAC 
ATTATGGCTG CTCAATCAAA AGAAGCTATT GGTGTCCTTG CCGTAACCAT TGCTTCTGCC 
GACTCTTTGA AAGGCTCAGA TTTCATTACC AATACTGTCG ATCCTTATAT TGTTATGACT 
ACCGAAGATG CTGTGCCTGG TACAGATGAA GAAGTGCGTA CATCTATCAA ATCAAATGTT 
AAAAATCCAC GTTGGAACGA AACCAAATAT CTATTATTAA ACACCTTAGA GCAAAAGTTA 
AACTTAAAGT GCTTTGACTT CAATGATGTA AGAAAAGATA CCGTAATTGG TGATCTTCAA 
CTTGACTTGG CAGATTTACT ACAAAACCCT GTTTTGGATA ATCAAACTGC TGAATTAAGA 
TCCGGTACAA AATCAAAAGG TATTTTACAT TATTCCTTAC ACTGGTTCCC TGTGAAAGAA 
GATAAATCAG AGGAAAAAGC AGTTGAGCGT GCCGAAGCTA AGGCCAAGGG CAAGAAAGAA 
GATGAAAACG AGGACACTAC TGAAAAAGAA GAAGACGAGA ATGAAGAAAG TTCTCAAACT 
GATGTCGGGA TTGCCAAGAT CACTTTACAA AAGGTCAAAT ATCTGGATAC AACCAGTTCT 
ATGACCGGTA GCTTGAGCCC ATGTGCTGAA TTATTCATTG ATGGACAAAA AGTAAAGAGC 
TATAGAACTT TGAGACGTAT CAATGAGCCA TCTTGGAATG AGACCATCGA AGTTTTGGTT 
CCATCAAAAT CTAACTCTAA GTTTGTCCTA AAAATATTCG ATGACAGAAT GAATGGTAAG 
GCGCTGATCT GTGAGTATTC ATCTTCTTTA GATGATATAA TGACTACTTT AGACACTGCT 
CAAGAGTTTG TTAAAGGCTC ACCACAAGGT GACATTTATT TGGATGTTTC TTGGAAATCA 
ATTGAAATGA CCGGAGCTTT TGCCGCTGCA AACTCTGTAA GCGAACCTAT TGGTTGTATT 
AAGCTAGACG TTAAGGATGC CATTATCAAG GGTGACTTAT CCGGTGTAGG GGATGTTGAT 
CCATATTACA CCGTATCGTT GAATAGACGT GTTCTTTACA AGTCCATATA TCATTCTGAT 
ACGGATCATC CCATTTTTGA CAACAGCACC TACGTTCCTA TCTTCTCTCC AAATCAAATT 
TTGACTCTCG AATTTCATGA TTATCAAAAG ATCGGCAAAG ACCGTTTCAT TGGCTCTGTA 
CAAATTCCTA CATCAAATGT TTTCAAAAAA GATCCTAAAT CAGGAAAATA TGTTGGGAAT 
AATGGCAAAG AAGAAATTTC AAAACTAAAA TTAAAAGACC ACGAACACAA AGTTACCGAA 
AGCATTGTCA ATGTTTCAAC AACATTTATC CCAATCAATC TGGTGTATTC CCCTGAGGAG 
TTGGTGAATG TTGAGAAACT AGAAAAGGAG TTGAAGGAAA AGAAGAAAAA ATTCGAAGCT 
ACCCAAGAAG AAAACGAGCA AGAGATGGAA AAAAATCCAA AGGAATGGGA AGTTGCCGAG 
ATCGAAGACC CATTTGACAG CGATGAAAAA AAAATAAACA GGAAGGCCAA GTTATCTTTA 
AACGAGTTGA TCAAGCAAAA ATCTGGTATT TTGTCTATGC AAATATTGGA AGGGACTTTG 
AGCCCATCCT CTGCTTACCT AGAAATCTTA GCGGATGACA TTTCGTACCC TGTATTCATT 
TGCATGAAAC CATCTCAAGG TAAACTAAAC TCGGAGATGG CAAATATTTT CATTAGAGAT 
TTGAATTACA GTAAACTACA TTTTAGAGTA TCGAAGAAAC ATATTGCCAA AGATTCAGAT 
GATGTCATAT CCGAAACTTC CTATAGTACA TTGAAGCTAC TAAAGCAAGC TTACGAAGAG 
CCCATGTGGT TAAACTTCAA TGGGTCTAAA ATGAAGGTAA GATTTTTGTA CACGCCCACT 
AGCGTGAAAC TGCCTAGCAG TGAAAGTGTT GAAGACACTG GTTATTTGAA TATAAAGCTT 
ATTTCCGGAC ACGGTCTGAA GTCCGCAGAT AGGAATGGCT ATTCAGATCC ATTTGTTCAC 
ATCTTTGTCA ATGATAAAAA AGTTTTCAAA TCGAACATTA AAAAGAAAAC ATTGGATCCC 
GTATGGAACG AAGATGCTAA AATACCAATC CTTTCAAGAA GTAAGAATCA AGTCATATTT 
AATGTTCTTG ATTGGGATCG TGCAGGTGAT AATGACGACT TAGGCCAAGC TTCACTTGAC 
GTTTCCTCAT TAGAAGTTGG TAAAACTTAC AACTGGAATT TGAATTTAAA CACACAAGGA 
AGTATCAAAT TACAAGGTTC ATTCAACCCA GAATATATCA AGCCAAGTTT TGATATCGTG 
AAAGGCGGTA TCACTGATAA GCCGATGAAA ATAGCCAGTG GTGCAGCCCA TGCAACTGTT 
GGCATAGCTG GTACTGGTAT AGGAGCAGCA ACAGGAGTTG CCACTGGTGG TTTAAAGAAA 
GGTGGTCACC TTCTAAAATC TCTAGGTGGC AATCCAATGA AAAGAAGCAA GAGCAGCAAT 
GGAAATGAGT CCAACGGTGC AAAAAAATCA TCAGAGAAAA AATCTTTTGA TAGGAGATCC 
CCAAGTAATT TGAATAGCAC TAGTGTAACA CCAAGAGCTT CACTAGACTA TGATCCATCA 
GTACCTAACA CAAGTTACGC GCCCGTTCAA AGCGCATCTC CTGTAGTCAA GCCAACTGAC 
AACACTTCTA GCTCAAGCAA CAAAAAAGAT ACCCCTAGTA GCAACTCTAG AGGACATTCT 
CGTGCAAGCA GTTTTGCGCG TACTTTAGCT CCTCATGGCA CTTACAATGG TTTTATTACC 
GTGGTTGCTG CGGAAAACGT TGCCAAGCAT GTTCAAATTA AGATCTCTTT AACTCAAGGT 
GGTAGACTAA AACACATATA CAAAACGAAA AGCCAAAAAG CCAATAATGA TGGTGTTGCC 
GTATTTGATG AAGAGTGCTC GTTCAAGGCT TCTCCCGAAG CCAATTTGGT ACTGGGTGCA 
ATTTCCCATC AAAGACTATC GAGGGACAAA GATCTTGGTA TTGCTCAAAT CAACTTGGGT 
GACCCTCAAA TTCAACAAGA TGGCCAAATT TCTGTAAAAT TAGGAGACGG TCATCTGATT 
GTAAAGATTA ATTACGGTAA AGACAAGAAT GGTCAGGTAC CTCCCGTGCC AGAAGTTCCT 
CAAGAATACA CGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TCB3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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