MLP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MLP1
Entrez Gene ID	853970
Full Name	Mlp1p
Synonyms	MPL1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179885.1	NP_013021.1	Mlp1p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR286W	NP_985833.1
Saccharomyces cerevisiae (baker's yeast)	MLP1	NP_013021.1
Kluyveromyces lactis	KLLA0A00594g	XP_451024.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mlp1p and Mlp2p are necessary components of the nuclear basket and they embed the nuclear pore complex within a dynamic protein network
Title: The nuclear basket proteins Mlp1p and Mlp2p are part of a dynamic interactome including Esc1p and the proteasome.

Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export
Title: Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export.

The N-terminal domain of Nab2 (residues 1-97) mediates interactions with both the C-terminal globular domain of the nuclear pore-associated protein, myosin-like protein 1 (Mlp1), and the mRNA export factor, Gfd1.
Title: Structure of the N-terminal Mlp1-binding domain of the Saccharomyces cerevisiae mRNA-binding protein, Nab2.

Mlps link mRNA transcription and export
Title: Perinuclear Mlp proteins downregulate gene expression in response to a defect in mRNA export.

Mlps play important roles in regulating Ulp1 and subsequently affect sumoylation stasis, growth, and DNA repair
Title: Mlp-dependent anchorage and stabilization of a desumoylating enzyme is required to prevent clonal lethality.

The following MLP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03147	NM_001179885.1 Latest version!	Saccharomyces cerevisiae S288C Mlp1p (MLP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MLP1 ( NM_001179885.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013021.1 Saccharomyces cerevisiae S288C Mlp1p (MLP1), partial mRNA.

Price & Availability↑

CloneID	OSi03147
Clone ID Related Accession (Same CDS sequence)	NM_001179885.1
Accession Version	NM_001179885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mlp1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGGATC ATGATACTCC AATGGAAAGC ATACAAAATG GTGAAAATTC AGACGAAAGA 
TTGAACGCCA TTGCGTCTTT TTTCGGTTGC TCTTTAGAGC AGGTTAAATC ATTTGACGGT 
GATGTGGTAA AACACCTTAA CGATAAACTT TTACAGTTTA ATGAACTTAA ATCGGAGAAT 
CTAAAGGTTA CCGTCTCATT CGATGAATTG AAGGCTAGTT CTTTAAAGAA AATTGATGGT 
TTGAAGACAG AAATGGAAAA CGTTATAAGA GAAAATGATA AAATCCGAAA AGAGAGGAAT 
GATACTTTTG TTAAGTTCGA ATCTGTAGAA AATGAAAAGA TGAAATTATC AAGTGAGCTA 
GAGTTTGTGA AAAGGAAGCT CGATGATTTA ACTGAGGAGA AAAAGGAAAC CCAGAGTAAT 
CAACAGCGAA CCCTGAAAAT ACTGGATGAA AGACTAAAAG AAATAGAATT GGTCAGGGTT 
GAGAATAATC GCTCCAATAG TGAATGTAAG AAACTACGCT CTACAATAAT GGATTTAGAA 
ACAAAACAAC AGGGCTATAT TACTAATGAC CTGAATTCTA GAACTGAACT GGAAAGAAAA 
ACACAAGAAT TGACTTTATT GCAGTCAAAT AATGATTGGC TAGAAAAGGA GCTACGTTCA 
AAAAATGAAC AGTATCTCTC CTACAGACAA AAAACCGACA AAGTAATTCT AGATATCAGG 
AATGAATTAA ATCGTTTAAG GAATGATTTT CAAATGGAAA GAACGAATAA TGATGTTTTG 
AAGCAAAAAA ATAACGAATT GTCAAAATCT TTACAAGAAA AACTACTGGA AATCAAGGGT 
CTATCCGACT CCCTAAATTC CGAAAAGCAA GAATTTTCTG CAGAAATGTC CCTAAAGCAA 
CGTTTAGTGG ACCTTTTAGA ATCACAATTG AACGCTGTAA AAGAAGAATT GAACAGTATA 
AGAGAGTTGA ACACTGCAAA GGTAATAGCA GATGATTCAA AAAAACAAAC TCCCGAAAAT 
GAAGACTTAC TTAAGGAATT GCAGTTAACG AAAGAAAAAT TAGCACAATG CGAAAAAGAA 
TGTCTACGTT TATCCTCTAT AACTGACGAA GCAGATGAAG ATAATGAAAA TTTATCTGCA 
AAATCTAGTT CTGATTTTAT ATTCCTGAAG AAACAATTAA TTAAAGAAAG GCGTACCAAG 
GAACATCTTC AAAATCAAAT TGAAACATTC ATCGTAGAGT TGGAACATAA AGTGCCCATT 
ATAAACTCTT TCAAAGAAAG AACTGACATG TTGGAAAACG AATTGAATAA CGCTGCATTG 
TTACTAGAGC ATACATCGAA CGAGAAGAAT GCAAAGGTTA AGGAATTAAA TGCCAAAAAC 
CAAAAGCTAG TGGAATGTGA AAATGATCTT CAAACTTTAA CTAAACAACG TCTCGATCTA 
TGCCGTCAAA TACAATACCT TTTAATTACC AATTCTGTTT CTAATGACTC GAAGGGACCC 
TTACGTAAGG AAGAAATTCA ATTTATTCAA AACATTATGC AGGAAGACGA TAGTACTATC 
ACAGAATCTG ACTCTCAAAA AGTCGTAACT GAAAGACTAG TTGAATTCAA AAACATTATT 
CAATTACAAG AAAAAAATGC AGAACTTTTG AAAGTAGTAA GAAACTTAGC CGATAAGTTG 
GAATCGAAAG AAAAGAAATC TAAACAAAGT CTTCAGAAAA TCGAAAGTGA AACAGTAAAT 
GAGGCTAAAG AGGCTATAAT AACTTTAAAG AGTGAAAAAA TGGATCTAGA ATCAAGAATT 
GAGGAACTAC AGAAAGAGCT TGAAGAATTG AAAACTTCTG TTCCCAACGA AGATGCGTCA 
TACAGCAATG TAACTATAAA ACAGTTAACC GAAACTAAGA GAGACCTCGA ATCTCAAGTA 
CAAGACTTGC AAACTCGTAT CTCGCAAATT ACTAGGGAGT CTACTGAAAA TATGTCACTT 
TTAAACAAGG AGATACAGGA CCTGTATGAC AGCAAGAGCG ACATATCCAT TAAGCTTGGA 
AAGGAAAAAT CATCGAGAAT ATTGGCAGAG GAACGATTTA AACTACTTTC GAATACGTTA 
GATCTAACTA AAGCTGAGAA CGACCAACTG CGCAAAAGGT TTGATTATTT ACAGAATACT 
ATTTTAAAAC AAGATTCCAA AACACACGAG ACACTTAATG AATACGTTTC CTGTAAATCT 
AAGTTAAGCA TTGTTGAAAC AGAATTATTG AACCTGAAAG AAGAACAGAA ATTAAGAGTT 
CATTTAGAAA AGAACTTGAA ACAAGAACTG AATAAACTCT CCCCTGAAAA GGACAGTTTA 
CGCATCATGG TAACTCAATT ACAAACTTTA CAAAAGGAGC GTGAAGATCT ATTGGAAGAG 
ACTAGGAAAT CATGTCAAAA GAAAATAGAT GAACTTGAAG ATGCTCTCAG CGAACTTAAA 
AAGGAAACTT CTCAAAAAGA CCATCATATC AAACAGCTGG AAGAAGACAA CAATTCAAAT 
ATAGAATGGT ACCAAAATAA AATCGAAGCT TTGAAGAAAG ATTATGAATC AGTAATAACT 
TCTGTAGATA GTAAGCAAAC TGACATTGAG AAATTACAAT ATAAAGTCAA ATCACTAGAA 
AAGGAAATCG AGGAGGACAA GATTCGTTTA CATACTTATA ATGTTATGGA TGAAACAATT 
AACGATGATT CCCTACGCAA GGAGTTGGAA AAATCCAAGA TTAACTTAAC TGATGCTTAT 
TCACAAATCA AAGAATACAA GGATCTCTAC GAGACTACCT CTCAGTCTTT GCAGCAAACG 
AATTCTAAAT TGGATGAATC TTTCAAGGAC TTTACTAACC AGATTAAAAA CCTAACTGAT 
GAAAAAACTA GTTTGGAGGA TAAGATTTCG CTTCTAAAAG AGCAAATGTT TAATTTGAAT 
AATGAGCTAG ATTTGCAGAA AAAAGGGATG GAAAAAGAAA AAGCTGACTT TAAGAAAAGG 
ATATCAATTT TACAGAACAA TAATAAAGAA GTCGAAGCTG TTAAGTCCGA ATATGAATCG 
AAGTTATCAA AAATCCAAAA CGACCTTGAT CAACAAACTA TATATGCTAA TACTGCGCAA 
AACAACTATG AACAAGAACT ACAGAAACAT GCAGATGTTT CTAAGACGAT TAGTGAATTA 
AGAGAGCAAT TACATACGTA CAAAGGTCAA GTTAAGACCC TGAACTTATC GCGTGATCAA 
CTGGAGAATG CTCTGAAAGA AAACGAAAAG AGTTGGTCCT CCCAGAAGGA ATCTTTATTA 
GAACAGCTAG ATTTATCGAA TTCTCGTATT GAGGATTTAT CCTCCCAAAA TAAACTATTG 
TATGATCAAA TACAAATCTA CACAGCTGCG GACAAAGAAG TCAATAATTC GACAAACGGA 
CCTGGTTTGA ATAATATTTT AATTACACTA CGTCGCGAAA GGGATATTCT TGATACAAAA 
GTGACGGTGG CTGAAAGAGA TGCAAAAATG TTGAGACAAA AAATTTCTTT GATGGATGTT 
GAATTACAAG ATGCTCGTAC TAAGCTAGAT AATTCAAGAG TTGAAAAGGA AAATCATTCT 
TCCATTATTC AACAGCATGA CGACATTATG GAGAAATTAA ATCAATTAAA TCTATTAAGA 
GAAAGTAACA TAACATTGCG GAATGAGCTG GAAAACAACA ATAACAAGAA GAAGGAACTG 
CAATCTGAAT TAGATAAATT GAAGCAAAAT GTTGCGCCTA TCGAGTCCGA ATTGACAGCC 
TTGAAATATT CTATGCAAGA AAAAGAGCAA GAGCTCAAAT TAGCTAAAGA AGAGGTTCAT 
CGTTGGAAAA AGCGCTCACA AGACATATTG GAGAAACATG AACAATTGAG CTCAAGCGAT 
TATGAGAAGC TAGAAAGCGA GATAGAAAAT TTGAAGGAGG AACTAGAAAA TAAGGAGCGT 
CAAGGAGCGG AAGCCGAGGA AAAATTTAAC AGGCTGAGAA GACAAGCGCA AGAGAGATTA 
AAAACATCAA AACTCTCACA GGACTCATTG ACTGAACAAG TAAATAGTCT AAGGGATGCA 
AAGAACGTGT TGGAAAATTC CTTGAGTGAG GCAAACGCGA GAATCGAAGA GTTACAAAAT 
GCAAAAGTAG CACAAGGTAA CAACCAGTTA GAAGCAATAA GAAAATTACA AGAAGACGCA 
GAAAAGGCTT CAAGAGAGCT TCAAGCCAAG TTAGAAGAAA GTACGACTTC TTACGAATCT 
ACGATAAACG GCTTAAATGA AGAGATTACA ACATTAAAAG AAGAAATTGA AAAACAAAGG 
CAAATCCAGC AACAGTTACA AGCTACATCT GCAAATGAAC AAAATGACTT ATCTAACATA 
GTTGAGTCTA TGAAAAAGTC TTTTGAAGAA GATAAAATCA AATTCATCAA AGAAAAAACC 
CAAGAAGTTA ATGAAAAAAT ACTCGAGGCC CAAGAAAGGC TAAATCAACC TTCCAATATC 
AATATGGAGG AGATTAAAAA AAAATGGGAA TCTGAGCACG AACAGGAAGT ATCTCAAAAG 
ATTCGCGAAG CTGAGGAAGC CCTCAAAAAG CGAATCAGAT TACCCACTGA GGAGAAAATT 
AATAAGATAA TCGAACGAAA GAAGGAGGAA TTGGAAAAAG AGTTTGAAGA AAAGGTTGAG 
GAGAGAATAA AATCAATGGA ACAATCTGGA GAAATAGACG TGGTGCTTCG AAAACAGCTA 
GAAGCTAAGG TTCAAGAGAA ACAAAAGGAA TTGGAAAACG AGTATAACAA AAAATTACAA 
GAAGAACTCA AAGATGTACC ACACTCAAGT CATATCTCAG ATGATGAAAG GGACAAATTA 
CGAGCAGAAA TCGAAAGCAG GTTGAGGGAG GAGTTCAACA ATGAACTGCA AGCCATAAAG 
AAGAAATCCT TCGACGAAGG AAAGCAACAA GCAATGATGA AAACTACCCT TTTGGAAAGA 
AAACTTGCCA AGATGGAATC TCAATTGTCA GAAACAAAAC AAAGTGCCGA GAGTCCTCCG 
AAATCTGTTA ACAATGTACA AAATCCATTA CTAGGATTAC CTAGGAAAAT CGAAGAGAAT 
TCAAATTCAC CATTCAATCC GTTACTTTCC GGTGAAAAAC TCTTAAAGCT AAATTCTAAG 
TCTTCATCAG GTGGATTTAA CCCTTTTACC TCGCCATCCC CAAATAAGCA CTTACAAAAT 
GATAATGACA AAAGGGAGTC GTTGGCTAAC AAGACAGATC CACCAACTCA TTTGGAACCC 
AGCTTCAACA TTCCCGCCTC AAGGGGTCTA ATATCTTCAT CTTCCACTTT GTCAACTGAT 
ACAAATGATG AAGAACTTAC TAGCAACAAT CCTGCCCAAA AGGATTCATC GAACAGAAAT 
GTTCAATCGG AAGAGGATAC AGAAAAAAAG AAAGAGGGAG AACCTGTTAA AAGAGGAGAG 
GCAATAGAAG AGCAGACGAA ATCCAACAAG CGACCTATTG ATGAGGTAGG AGAGCTGAAA 
AATGATGAAG ACGACACTAC AGAAAACATT AATGAGTCAA AAAAGATCAA GACTGAAGAT 
GAGGAAGAAA AAGAAACCGA TAAGGTGAAT GACGAGAACA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013021.1
CDS	1..5628
Translation	MSDHDTPMESIQNGENSDERLNAIASFFGCSLEQVKSFDGDVVKHLNDKLLQFNELKSENLKVTVSFDELKASSLKKIDGLKTEMENVIRENDKIRKERNDTFVKFESVENEKMKLSSELEFVKRKLDDLTEEKKETQSNQQRTLKILDERLKEIELVRVENNRSNSECKKLRSTIMDLETKQQGYITNDLNSRTELERKTQELTLLQSNNDWLEKELRSKNEQYLSYRQKTDKVILDIRNELNRLRNDFQMERTNNDVLKQKNNELSKSLQEKLLEIKGLSDSLNSEKQEFSAEMSLKQRLVDLLESQLNAVKEELNSIRELNTAKVIADDSKKQTPENEDLLKELQLTKEKLAQCEKECLRLSSITDEADEDNENLSAKSSSDFIFLKKQLIKERRTKEHLQNQIETFIVELEHKVPIINSFKERTDMLENELNNAALLLEHTSNEKNAKVKELNAKNQKLVECENDLQTLTKQRLDLCRQIQYLLITNSVSNDSKGPLRKEEIQFIQNIMQEDDSTITESDSQKVVTERLVEFKNIIQLQEKNAELLKVVRNLADKLESKEKKSKQSLQKIESETVNEAKEAIITLKSEKMDLESRIEELQKELEELKTSVPNEDASYSNVTIKQLTETKRDLESQVQDLQTRISQITRESTENMSLLNKEIQDLYDSKSDISIKLGKEKSSRILAEERFKLLSNTLDLTKAENDQLRKRFDYLQNTILKQDSKTHETLNEYVSCKSKLSIVETELLNLKEEQKLRVHLEKNLKQELNKLSPEKDSLRIMVTQLQTLQKEREDLLEETRKSCQKKIDELEDALSELKKETSQKDHHIKQLEEDNNSNIEWYQNKIEALKKDYESVITSVDSKQTDIEKLQYKVKSLEKEIEEDKIRLHTYNVMDETINDDSLRKELEKSKINLTDAYSQIKEYKDLYETTSQSLQQTNSKLDESFKDFTNQIKNLTDEKTSLEDKISLLKEQMFNLNNELDLQKKGMEKEKADFKKRISILQNNNKEVEAVKSEYESKLSKIQNDLDQQTIYANTAQNNYEQELQKHADVSKTISELREQLHTYKGQVKTLNLSRDQLENALKENEKSWSSQKESLLEQLDLSNSRIEDLSSQNKLLYDQIQIYTAADKEVNNSTNGPGLNNILITLRRERDILDTKVTVAERDAKMLRQKISLMDVELQDARTKLDNSRVEKENHSSIIQQHDDIMEKLNQLNLLRESNITLRNELENNNNKKKELQSELDKLKQNVAPIESELTALKYSMQEKEQELKLAKEEVHRWKKRSQDILEKHEQLSSSDYEKLESEIENLKEELENKERQGAEAEEKFNRLRRQAQERLKTSKLSQDSLTEQVNSLRDAKNVLENSLSEANARIEELQNAKVAQGNNQLEAIRKLQEDAEKASRELQAKLEESTTSYESTINGLNEEITTLKEEIEKQRQIQQQLQATSANEQNDLSNIVESMKKSFEEDKIKFIKEKTQEVNEKILEAQERLNQPSNINMEEIKKKWESEHEQEVSQKIREAEEALKKRIRLPTEEKINKIIERKKEELEKEFEEKVEERIKSMEQSGEIDVVLRKQLEAKVQEKQKELENEYNKKLQEELKDVPHSSHISDDERDKLRAEIESRLREEFNNELQAIKKKSFDEGKQQAMMKTTLLERKLAKMESQLSETKQSAESPPKSVNNVQNPLLGLPRKIEENSNSPFNPLLSGEKLLKLNSKSSSGGFNPFTSPSPNKHLQNDNDKRESLANKTDPPTHLEPSFNIPASRGLISSSSTLSTDTNDEELTSNNPAQKDSSNRNVQSEEDTEKKKEGEPVKRGEAIEEQTKSNKRPIDEVGELKNDEDDTTENINESKKIKTEDEEEKETDKVNDENSI

Target ORF information:

RefSeq Version	NM_001179885.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mlp1p (MLP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179885.1

ORF Insert Sequence:

ATGTCGGATC ATGATACTCC AATGGAAAGC ATACAAAATG GTGAAAATTC AGACGAAAGA 
TTGAACGCCA TTGCGTCTTT TTTCGGTTGC TCTTTAGAGC AGGTTAAATC ATTTGACGGT 
GATGTGGTAA AACACCTTAA CGATAAACTT TTACAGTTTA ATGAACTTAA ATCGGAGAAT 
CTAAAGGTTA CCGTCTCATT CGATGAATTG AAGGCTAGTT CTTTAAAGAA AATTGATGGT 
TTGAAGACAG AAATGGAAAA CGTTATAAGA GAAAATGATA AAATCCGAAA AGAGAGGAAT 
GATACTTTTG TTAAGTTCGA ATCTGTAGAA AATGAAAAGA TGAAATTATC AAGTGAGCTA 
GAGTTTGTGA AAAGGAAGCT CGATGATTTA ACTGAGGAGA AAAAGGAAAC CCAGAGTAAT 
CAACAGCGAA CCCTGAAAAT ACTGGATGAA AGACTAAAAG AAATAGAATT GGTCAGGGTT 
GAGAATAATC GCTCCAATAG TGAATGTAAG AAACTACGCT CTACAATAAT GGATTTAGAA 
ACAAAACAAC AGGGCTATAT TACTAATGAC CTGAATTCTA GAACTGAACT GGAAAGAAAA 
ACACAAGAAT TGACTTTATT GCAGTCAAAT AATGATTGGC TAGAAAAGGA GCTACGTTCA 
AAAAATGAAC AGTATCTCTC CTACAGACAA AAAACCGACA AAGTAATTCT AGATATCAGG 
AATGAATTAA ATCGTTTAAG GAATGATTTT CAAATGGAAA GAACGAATAA TGATGTTTTG 
AAGCAAAAAA ATAACGAATT GTCAAAATCT TTACAAGAAA AACTACTGGA AATCAAGGGT 
CTATCCGACT CCCTAAATTC CGAAAAGCAA GAATTTTCTG CAGAAATGTC CCTAAAGCAA 
CGTTTAGTGG ACCTTTTAGA ATCACAATTG AACGCTGTAA AAGAAGAATT GAACAGTATA 
AGAGAGTTGA ACACTGCAAA GGTAATAGCA GATGATTCAA AAAAACAAAC TCCCGAAAAT 
GAAGACTTAC TTAAGGAATT GCAGTTAACG AAAGAAAAAT TAGCACAATG CGAAAAAGAA 
TGTCTACGTT TATCCTCTAT AACTGACGAA GCAGATGAAG ATAATGAAAA TTTATCTGCA 
AAATCTAGTT CTGATTTTAT ATTCCTGAAG AAACAATTAA TTAAAGAAAG GCGTACCAAG 
GAACATCTTC AAAATCAAAT TGAAACATTC ATCGTAGAGT TGGAACATAA AGTGCCCATT 
ATAAACTCTT TCAAAGAAAG AACTGACATG TTGGAAAACG AATTGAATAA CGCTGCATTG 
TTACTAGAGC ATACATCGAA CGAGAAGAAT GCAAAGGTTA AGGAATTAAA TGCCAAAAAC 
CAAAAGCTAG TGGAATGTGA AAATGATCTT CAAACTTTAA CTAAACAACG TCTCGATCTA 
TGCCGTCAAA TACAATACCT TTTAATTACC AATTCTGTTT CTAATGACTC GAAGGGACCC 
TTACGTAAGG AAGAAATTCA ATTTATTCAA AACATTATGC AGGAAGACGA TAGTACTATC 
ACAGAATCTG ACTCTCAAAA AGTCGTAACT GAAAGACTAG TTGAATTCAA AAACATTATT 
CAATTACAAG AAAAAAATGC AGAACTTTTG AAAGTAGTAA GAAACTTAGC CGATAAGTTG 
GAATCGAAAG AAAAGAAATC TAAACAAAGT CTTCAGAAAA TCGAAAGTGA AACAGTAAAT 
GAGGCTAAAG AGGCTATAAT AACTTTAAAG AGTGAAAAAA TGGATCTAGA ATCAAGAATT 
GAGGAACTAC AGAAAGAGCT TGAAGAATTG AAAACTTCTG TTCCCAACGA AGATGCGTCA 
TACAGCAATG TAACTATAAA ACAGTTAACC GAAACTAAGA GAGACCTCGA ATCTCAAGTA 
CAAGACTTGC AAACTCGTAT CTCGCAAATT ACTAGGGAGT CTACTGAAAA TATGTCACTT 
TTAAACAAGG AGATACAGGA CCTGTATGAC AGCAAGAGCG ACATATCCAT TAAGCTTGGA 
AAGGAAAAAT CATCGAGAAT ATTGGCAGAG GAACGATTTA AACTACTTTC GAATACGTTA 
GATCTAACTA AAGCTGAGAA CGACCAACTG CGCAAAAGGT TTGATTATTT ACAGAATACT 
ATTTTAAAAC AAGATTCCAA AACACACGAG ACACTTAATG AATACGTTTC CTGTAAATCT 
AAGTTAAGCA TTGTTGAAAC AGAATTATTG AACCTGAAAG AAGAACAGAA ATTAAGAGTT 
CATTTAGAAA AGAACTTGAA ACAAGAACTG AATAAACTCT CCCCTGAAAA GGACAGTTTA 
CGCATCATGG TAACTCAATT ACAAACTTTA CAAAAGGAGC GTGAAGATCT ATTGGAAGAG 
ACTAGGAAAT CATGTCAAAA GAAAATAGAT GAACTTGAAG ATGCTCTCAG CGAACTTAAA 
AAGGAAACTT CTCAAAAAGA CCATCATATC AAACAGCTGG AAGAAGACAA CAATTCAAAT 
ATAGAATGGT ACCAAAATAA AATCGAAGCT TTGAAGAAAG ATTATGAATC AGTAATAACT 
TCTGTAGATA GTAAGCAAAC TGACATTGAG AAATTACAAT ATAAAGTCAA ATCACTAGAA 
AAGGAAATCG AGGAGGACAA GATTCGTTTA CATACTTATA ATGTTATGGA TGAAACAATT 
AACGATGATT CCCTACGCAA GGAGTTGGAA AAATCCAAGA TTAACTTAAC TGATGCTTAT 
TCACAAATCA AAGAATACAA GGATCTCTAC GAGACTACCT CTCAGTCTTT GCAGCAAACG 
AATTCTAAAT TGGATGAATC TTTCAAGGAC TTTACTAACC AGATTAAAAA CCTAACTGAT 
GAAAAAACTA GTTTGGAGGA TAAGATTTCG CTTCTAAAAG AGCAAATGTT TAATTTGAAT 
AATGAGCTAG ATTTGCAGAA AAAAGGGATG GAAAAAGAAA AAGCTGACTT TAAGAAAAGG 
ATATCAATTT TACAGAACAA TAATAAAGAA GTCGAAGCTG TTAAGTCCGA ATATGAATCG 
AAGTTATCAA AAATCCAAAA CGACCTTGAT CAACAAACTA TATATGCTAA TACTGCGCAA 
AACAACTATG AACAAGAACT ACAGAAACAT GCAGATGTTT CTAAGACGAT TAGTGAATTA 
AGAGAGCAAT TACATACGTA CAAAGGTCAA GTTAAGACCC TGAACTTATC GCGTGATCAA 
CTGGAGAATG CTCTGAAAGA AAACGAAAAG AGTTGGTCCT CCCAGAAGGA ATCTTTATTA 
GAACAGCTAG ATTTATCGAA TTCTCGTATT GAGGATTTAT CCTCCCAAAA TAAACTATTG 
TATGATCAAA TACAAATCTA CACAGCTGCG GACAAAGAAG TCAATAATTC GACAAACGGA 
CCTGGTTTGA ATAATATTTT AATTACACTA CGTCGCGAAA GGGATATTCT TGATACAAAA 
GTGACGGTGG CTGAAAGAGA TGCAAAAATG TTGAGACAAA AAATTTCTTT GATGGATGTT 
GAATTACAAG ATGCTCGTAC TAAGCTAGAT AATTCAAGAG TTGAAAAGGA AAATCATTCT 
TCCATTATTC AACAGCATGA CGACATTATG GAGAAATTAA ATCAATTAAA TCTATTAAGA 
GAAAGTAACA TAACATTGCG GAATGAGCTG GAAAACAACA ATAACAAGAA GAAGGAACTG 
CAATCTGAAT TAGATAAATT GAAGCAAAAT GTTGCGCCTA TCGAGTCCGA ATTGACAGCC 
TTGAAATATT CTATGCAAGA AAAAGAGCAA GAGCTCAAAT TAGCTAAAGA AGAGGTTCAT 
CGTTGGAAAA AGCGCTCACA AGACATATTG GAGAAACATG AACAATTGAG CTCAAGCGAT 
TATGAGAAGC TAGAAAGCGA GATAGAAAAT TTGAAGGAGG AACTAGAAAA TAAGGAGCGT 
CAAGGAGCGG AAGCCGAGGA AAAATTTAAC AGGCTGAGAA GACAAGCGCA AGAGAGATTA 
AAAACATCAA AACTCTCACA GGACTCATTG ACTGAACAAG TAAATAGTCT AAGGGATGCA 
AAGAACGTGT TGGAAAATTC CTTGAGTGAG GCAAACGCGA GAATCGAAGA GTTACAAAAT 
GCAAAAGTAG CACAAGGTAA CAACCAGTTA GAAGCAATAA GAAAATTACA AGAAGACGCA 
GAAAAGGCTT CAAGAGAGCT TCAAGCCAAG TTAGAAGAAA GTACGACTTC TTACGAATCT 
ACGATAAACG GCTTAAATGA AGAGATTACA ACATTAAAAG AAGAAATTGA AAAACAAAGG 
CAAATCCAGC AACAGTTACA AGCTACATCT GCAAATGAAC AAAATGACTT ATCTAACATA 
GTTGAGTCTA TGAAAAAGTC TTTTGAAGAA GATAAAATCA AATTCATCAA AGAAAAAACC 
CAAGAAGTTA ATGAAAAAAT ACTCGAGGCC CAAGAAAGGC TAAATCAACC TTCCAATATC 
AATATGGAGG AGATTAAAAA AAAATGGGAA TCTGAGCACG AACAGGAAGT ATCTCAAAAG 
ATTCGCGAAG CTGAGGAAGC CCTCAAAAAG CGAATCAGAT TACCCACTGA GGAGAAAATT 
AATAAGATAA TCGAACGAAA GAAGGAGGAA TTGGAAAAAG AGTTTGAAGA AAAGGTTGAG 
GAGAGAATAA AATCAATGGA ACAATCTGGA GAAATAGACG TGGTGCTTCG AAAACAGCTA 
GAAGCTAAGG TTCAAGAGAA ACAAAAGGAA TTGGAAAACG AGTATAACAA AAAATTACAA 
GAAGAACTCA AAGATGTACC ACACTCAAGT CATATCTCAG ATGATGAAAG GGACAAATTA 
CGAGCAGAAA TCGAAAGCAG GTTGAGGGAG GAGTTCAACA ATGAACTGCA AGCCATAAAG 
AAGAAATCCT TCGACGAAGG AAAGCAACAA GCAATGATGA AAACTACCCT TTTGGAAAGA 
AAACTTGCCA AGATGGAATC TCAATTGTCA GAAACAAAAC AAAGTGCCGA GAGTCCTCCG 
AAATCTGTTA ACAATGTACA AAATCCATTA CTAGGATTAC CTAGGAAAAT CGAAGAGAAT 
TCAAATTCAC CATTCAATCC GTTACTTTCC GGTGAAAAAC TCTTAAAGCT AAATTCTAAG 
TCTTCATCAG GTGGATTTAA CCCTTTTACC TCGCCATCCC CAAATAAGCA CTTACAAAAT 
GATAATGACA AAAGGGAGTC GTTGGCTAAC AAGACAGATC CACCAACTCA TTTGGAACCC 
AGCTTCAACA TTCCCGCCTC AAGGGGTCTA ATATCTTCAT CTTCCACTTT GTCAACTGAT 
ACAAATGATG AAGAACTTAC TAGCAACAAT CCTGCCCAAA AGGATTCATC GAACAGAAAT 
GTTCAATCGG AAGAGGATAC AGAAAAAAAG AAAGAGGGAG AACCTGTTAA AAGAGGAGAG 
GCAATAGAAG AGCAGACGAA ATCCAACAAG CGACCTATTG ATGAGGTAGG AGAGCTGAAA 
AATGATGAAG ACGACACTAC AGAAAACATT AATGAGTCAA AAAAGATCAA GACTGAAGAT 
GAGGAAGAAA AAGAAACCGA TAAGGTGAAT GACGAGAACA GTATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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MLP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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